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| 1 | 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents显示文摘Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department visits.Recurrent syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up. | Cheng Wang Yaqi Li Ying Liao Hong Tian Min Huang Xiangyu Dong Lin Shi Jinghui Sun Hongfang Jin Junbao Du Jindou An Jie Chen Mingwu Chen Qi Chen Sun Chen Yonghong Chen Zhi Chen Adolphus Kai-tung Chau Junbao Du Zhongdong Du Junkai Duan Hongyu Duan Xiangyu Dong Lin Feng Lijun Fu Fangqi Gong Yonghao Gui Ling Han Zhenhui Han Bing He Zhixu He Xiufen Hu Yimin Hua Guoying Huang Min Huang Ping Huang Yujuan Huang Hongfang Jin Mei Jin Bo Li Fen Li Tao Li Xiaohui Li Xiaoyan Liu Yan Li Haitao Lv Tiewei Lv Zipu Li Luyi Ma Silin Pan Yusheng Pang Hua Peng Yuming Qin Jie Shen Lin Shi Kun Sun Jinghui Sun Hong Tian Jie Tian Cheng Wang Hong Wang Lei Wang Jinju Wang Wendi Wang Yuli Wang Rongzhou Wu Tianhe Xia Yanyan Xiao Chunhong Xie Yanlin Xing Zhenyu Xiong Baoyuan Xu Yi Xu Hui Yan Shiwei Yang Qijian Yi Xia Yu Xianyi Yu Yue Yuan Hongyan Zhang Huili Zhang Li Zhang Qingyou Zhang Xi Zhang Yanmin Zhang Zhiwei Zhang Cuifen Zhao Bin Zhou Hua Zhu | 2018 | Science Bulletin2018，63，23： | 54 |
| 2 | Resveratrol Induces Apoptosis and Autophagy in T-cell Acute Lymphoblastic Leukemia Cells by Inhibiting Akt/mTOR and Activating p38-MAPK显示文摘Objective To explore the effects of resveratrol-induced apoptosis and autophagy in T-cell acute lymphoblastic leukemia(T-ALL) cells and potential molecular mechanisms. Methods The anti-proliferation effect of resveratrol-induced, apoptosis and autophagy on T-ALL cells were detected by using MTT test, immunofluorescence, electronic microscope, and flow cytometry, respectively. Western blotting was performed for detecting changes of apoptosis-associated proteins, cell cycle regulatory proteins and state of activation of Akt, mTOR, p70S6K, 4E-BP1, and p38-MAPK. Results Resveratrol inhibited the proliferation and induced apoptosis and autophagy in T-ALL cells in a dose and time-dependent manner. It also induced cell cycle arrest at G0/G1 phase via up regulating cyclin-dependent kinase(CDK) inhibitors p21 and p27 and down regulating cyclin A and cyclin D1. Western blotting revealed that resveratrol significantly decreased the expression of antiapoptotic proteins(Mcl-1 and Bcl-2) and increased the expression of proapoptotic proteins(Bax, Bim, and Bad), and induced cleaved-caspase-3 in a time-dependent manner. Significant increase in ratio of LC3-II/LC3-I and Beclin 1 was also detected. Furthermore, resveratrol induced significant dephosphorylation of Akt, mTOR, p70S6K, and 4E-BP1, but enhanced specific phosphorylation of p38-MAPK which could be blocked by SB203580. When autophagy was suppressed by 3-MA, apoptosis in T-ALL cells induced by resveratrol was enhanced.Conclusion Our findings have suggested that resveratrol induces cell cycle arrest,apoptosis,and autophagy in T-ALL cells through inhibiting Akt/mTOR/p70S6K/4E-BP1 and activating p38-MAPK signaling pathways.Autophagy might play a role as a self-defense mechanism in T-ALL cells treated by resveratrol.Therefore,the reasonable inhibition of autophagy in T-ALL cells may serve as a promising strategy for resveratrol induced apoptosis and can be used as adjuvant chemotherapy for T-ALL. | GE Jiao LIU Yan LI Qiang GUO Xia GU Ling MA Zhi Gui ZHU Yi Ping | 2013 | Biomedical and Environmental Sciences2013，26，11： | 38 |
| 3 | OsGLU3, a Putative Membrane-Bound Endo-1, 4-Beta-Glucanase, Is Required for Root Cell Elongation and Division in Rice (Oryza sativa L.)显示文摘植物根由延伸通过土壤移动。这对他们抛锚的能力重要植物并且从土壤获得水和矿物质。为了识别新基因,在米饭在根延伸包含了,我们屏蔽了乙醇甲烷 sulfonate (他们)-mutagenized 米饭图书馆,并且孤立短根异种, Osglu3-1。基于地图的克隆结果证明异种由于在 OsGLU3 的一个点变化,它编码通常认为的膜界限 endo-1,4 -- glucanase。Osglu3-1 在它的根房间墙,更短的根房间长度,和由 OsCYCB1,1 的限制表示设想了的一个稍微更小的根分裂组织中显示了不太水晶的纤维素内容: GUS。葡萄糖的外长的申请能两个都压制更低的根房间墙纤维素内容和 Osglu3-1 的短根显型。一致地, OsGLU3 无所不在地在根尖端,侧面的根,和王冠根 primodia 与强壮的表示在各种各样的纸巾被表示。充分功能的 OsGLU3GFP 在根分裂组织和延伸地区在血浆膜,和 FM4-64-labeled 分隔空间被检测。我们也发现了那磷酸盐饥饿,一个环境压力,以一个 OsGLU3 家属方法调制根延伸的改变的房间墙纤维素内容。 | Jin-Wei Zhang Lei XU Yun-Rong Wu Xin-Ai Chen Yu Liu Shi-Hua Zhu Wo-Na Ding Ping Wu Ke-Ke Yi | 2012 | Molecular Plant2012，5，1： | 13 |
| 4 | Gene-Gene Interaction of GJB2, SOD2, and CAT on Occupational Noise-induced Hearing Loss in Chinese Han Population显示文摘The effects of genetic factors on the noise-induced hearing loss(NIHL)are still unclear.In the present study,eight single-nucleotide polymorphisms(SNPs)included rs1227049 and rs3802711(CDH23),rs1695(GSTP1),rs137852540(GJB2),rs2289274(PMCA2),rs4880(SOD2),rs7943316,and rs769214 within CAT that might associated with NIHL were further validated | WANG Sheng Li YU Lu Gang LIU Ren Ping ZHU Wan Zhan GAO Wei Min XUE Li Ping JIANG Xu ZHANG Ya Han YI Ding CHEN Dong ZHANG Yong Hong | 2014 | Biomedical and Environmental Sciences2014，27，12： | 12 |
| 5 | A cross-sectional study on HGV infection in a rural population显示文摘AbstractAIMTodeterminetheepidemiologicalcharacteristicsandclinicalsignificanceofHGVinfection,andtocomparewithHBVandHCVinfecti... | LING Bin Hua 1, ZHUANG Hui 1, CUI Yi Hui 1, AN Wen Feng 1, LI Zhi Jie 1, WANG Shu Ping 2 and ZHU Wan Fu 2 | 1998 | World Journal of Gastroenterology1998，4，6： | 10 |
| 6 | Triboelectric–Electromagnetic Hybrid Generator for Harvesting Blue Energy显示文摘Progress has been developed in harvesting lowfrequency and irregular blue energy using a triboelectric–electromagnetic hybrid generator in recent years. However,the design of the high-efficiency, mechanically durable hybrid structure is still challenging. In this study, we report a fully packaged triboelectric–electromagnetic hybrid generator(TEHG), in which magnets were utilized as the trigger to drive contact–separation-mode triboelectric nanogenerators(CS-TENGs) and coupled with copper coils to operate rotary freestanding-mode electromagnetic generators(RF-EMGs). The magnet pairs that produce attraction were used to transfer the external mechanical energy to the CS-TENGs, and packaging of the CS-TENGpart was achieved to protect it from the ambient environment. Under a rotatory speed of 100 rpm, the CS-TENGs enabled the TEHG to deliver an output voltage, current,and average power of 315.8 V, 44.6 μA, and ~ 90.7 μW,and the output of the RF-EMGs was 0.59 V, 1.78 m A, and 79.6 μW, respectively. The cylinder-like structure made the TEHG more easily driven by water flow and demonstrated to work as a practical power source to charge commercial capacitors. It can charge a 33μF capacitor from 0 to 2.1 V in 84 s, and the stored energy in the capacitor can drive an electronic thermometer and form a self-powered water-temperature sensing system. | Huiyun Shao Ping Cheng Ruixuan Chen Lingjie Xie Na Sun Qingqing Shen Xiaoping Chen Qianqian Zhu Yi Zhang Yina Liu Zhen Wen Xuhui Sun | 2018 | Nano-Micro Letters2018，10，3： | 9 |
| 7 | Synthesis of the novel PARP-1 inhibitor AG- 690/11026014 and its protective effects on angiotensin II-induced mouse cardiac remodeling显示文摘我们以前 poly 作为一篇小说识别了 AG-690/11026014 (6014 )( 自动数据处理核糖) 有效地阻止了血管收缩素 II (Ang II ) 的 polymerase-1 (PARP-1 ) 禁止者导致了 cardiomyocyte 肥大。在现在的学习,我们为 6014 报导了一条新合成线路,并且在 Ang 上调查了它的保护的效果在老鼠的导致 II 的心脏的改变和心脏的机能障碍和内在的机制。我们设计了一条新合成线路在 vivo 学习为这获得 6014 的足够的数量。C57BL/6J 老鼠与 Ang II 被灌输并且与 6014 对待(10, 30, 90 mg 日吗?? | Guo-shuai FENG Cui-ge ZHU Zhuo-ming LI Pan-xia WANG Yi HUANG Min LIU Ping HE Lan-lan LOU Shao-rui CHEN Pei-qing LIU | 2017 | Acta Pharmacologica Sinica2017，38，5： | 8 |
| 8 | Relationship between Maternal PBMC HBV cccDNA and HBV Serological Markers and its Effect on HBV Intrauterine Transmission显示文摘Objective To investigate the relationship between maternal peripheral blood mononuclear cells (PBMC) hepatitis B virus(HBV) covalenty closed circular deoxyribonucleic acid(cccDNA) and other HBV serological markers and its effects on HBV intrauterine transmission. Methods We enrolled 290 newborns and their hepatitis B surface antigen(HBsAg) positive mothers. HBV cccDNA in PBMC and HBV DNA in serum were detected by a real‐time PCR‐TaqM an probe while HBV serological markers were detected with an electrochemiluminescence immunoassay. Results There was a positive correlation between the levels of PBMC HBV cccD NA and serum HBV DNA and HBeA g(r = 0.436 and 0.403, P < 0.001). The detection rate of pattern A [‘HBsA g(+), HBeA g(+), and anti‐HBc(+)’] was significantly higher in the PBMC HBV cccD NA positive group than in the control group(χ^2 = 48.48, P < 0.001). There was a significant association between HBV intrauterine transmission and PBMC HBV cccD NA(χ^2 = 9.28, P = 0.002). In the presence of serum HBV DNA, HBeA g, and PBMC HBV cccD NA, the risk of HBV intrauterine transmission was three times higher(OR = 3.69, 95% CI: 1.30‐10.42) than that observed in their absence. The risk of HBV intrauterine transmission was the greatest(OR = 5.89, 95% CI: 2.35‐14.72) when both PBMC HBV cccD NA and pattern A were present. A Bayesian network model showed that maternal PBMC HBV cccD NA was directly related to HBV intrauterine transmission. Conclusion PBMC HBV cccDNA may be a direct risk factor for HBV intrauterine transmission. Our study suggests that serological markers could be combined with PBMC‐related markers in prenatal testing. | WANG Dan Dan YI Lin Zhu WU Li Na YANG Zhi Qing HAO Hai Yun SHI Xiao Hong WANG Bo FENG Shu Ying FENG Yong Liang WANG Su Ping | 2019 | Biomedical and Environmental Sciences2019，32，5： | 8 |
| 9 | Association of Overlapped and Un-overlapped Comorbidities with COVID-19 Severity and Treatment Outcomes: A Retrospective Cohort Study from Nine Provinces in China显示文摘Objective Several COVID-19 patients have overlapping comorbidities. The independent role of each component contributing to the risk of COVID-19 is unknown, and how some non-cardiometabolic comorbidities affect the risk of COVID-19 remains unclear.Methods A retrospective follow-up design was adopted. A total of 1,160 laboratory-confirmed patients were enrolled from nine provinces in China. Data on comorbidities were obtained from the patients’ medical records. Multivariable logistic regression models were used to estimate the odds ratio(OR) and 95% confidence interval(95% CI) of the associations between comorbidities(cardiometabolic or non-cardiometabolic diseases), clinical severity, and treatment outcomes of COVID-19.Results Overall, 158(13.6%) patients were diagnosed with severe illness and 32(2.7%) had unfavorable outcomes. Hypertension(2.87, 1.30–6.32), type 2 diabetes(T2 DM)(3.57, 2.32–5.49),cardiovascular disease(CVD)(3.78, 1.81–7.89), fatty liver disease(7.53, 1.96–28.96), hyperlipidemia(2.15, 1.26–3.67), other lung diseases(6.00, 3.01–11.96), and electrolyte imbalance(10.40, 3.00–26.10)were independently linked to increased odds of being severely ill. T2 DM(6.07, 2.89–12.75), CVD(8.47,6.03–11.89), and electrolyte imbalance(19.44, 11.47–32.96) were also strong predictors of unfavorable outcomes. Women with comorbidities were more likely to have severe disease on admission(5.46,3.25–9.19), while men with comorbidities were more likely to have unfavorable treatment outcomes(6.58, 1.46–29.64) within two weeks.Conclusion Besides hypertension, diabetes, and CVD, fatty liver disease, hyperlipidemia, other lung diseases, and electrolyte imbalance were independent risk factors for COVID-19 severity and poor treatment outcome. Women with comorbidities were more likely to have severe disease, while men with comorbidities were more likely to have unfavorable treatment outcomes. | MA Yan ZHU Dong Shan CHEN Ren Bo SHI Nan Nan LIU Si Hong FAN Yi Pin WU Gui Hui YANG Pu Ye BAI Jiang Feng CHEN Hong CHEN Li Ying FENG Qiao GUO Tuan Mao HOU Yong HU Gui Fen HU Xiao Mei HU Yun Hong HUANG Jin HUANG Qiu Hua HUANG Shao Zhen JI Liang JIN Hai Hao LEI Xiao LI Chun Yan LI Min Qing LI Qun Tang LI Xian Yong LIU Hong De LIU Jin Ping LIU Zhang MA Yu Ting MAO Ya MO Liu Fen NA Hui WANG Jing Wei SONG Fang Li SUN Sheng WANG Dong Ting WANG Ming Xuan WANG Xiao Yan WANG Yin Zhen WANG Yu Dong WU Wei WU Lan Ping XIAO Yan Hua XIE Hai Jun XU Hong Ming XU Shou Fang XUE Rui Xia YANG Chun YANG Kai Jun YUAN Sheng Li ZHANG Gong Qi ZHANG Jin Bo ZHANG Lin Song ZHAO Shu Sen ZHAO Wan Ying ZHENG Kai ZHOU Ying Chun ZHU Jun Teng ZHU Tian Qing ZHANG Hua Min WANG Yan Ping WANG Yong Yan 无 | 2020 | Biomedical and Environmental Sciences2020，33，12： | 8 |
| 10 | Cation-mixing stabilized layered oxide cathodes for sodium-ion batteries显示文摘Sodium-ion batteries are promising for large-scale energy storage due to sodium's low cost and infinite abundance. The most popular cathodes for sodium-ion batteries, i.e., the layered sodium-containing oxides, usually exhibit reversible host rearrangement between P-type and O-type stacking upon charge/discharge. Herein we demonstrate that such host rearrangement is unfavorable and can be suppressed by introducing transition-metal ions into sodium layers. The electrode with stabilized P3-type stacking delivers superior rate capability, high energy efficiency, and excellent cycling performance. Owing to the cation-mixing nature, it performs the lowest lattice strain among all reported cathodes for sodium-ion batteries. Our findings highlight the significance of a stable host for sodium-ion storage and moreover underline the fundamental distinction in material design strategy between lithium-and sodium-ion batteries. | Shaohua Guo Yang Sun Pan Liu Jin Yi Ping He Xiaoyu Zhang Yanbei Zhu Ryosuke Senga Kazu Suenaga Mingwei Chen Haoshen Zhou | 2018 | Science Bulletin2018，63，6： | 5 |
| 11 | A modified posterior wedge osteotomy with interbody fusion for the treatment of thoracolumbar kyphosis with Andersson lesions in ankylosing spondylitis:a 5-year follow-up study显示文摘Background:Andersson lesions(ALs),also known as spondylodiscities,destructive vertebral lesions and spinal pseudarthrosis,usually occur in patients with ankylosing spondylitis(AS).Inflammatory and traumatic causes have been proposed to define this lesion.Different surgical approaches including anterior,posterior,and combined anterior and posterior procedure have been used to address the complications,consisting of mechanical pain,kyphotic deformity,and neurologic deficits.However,the preferred surgical procedure remains controversial.The aim of this study was to illustrate the safety,efficacy,and feasibility of a modified posterior wedge osteotomy for the ALs with kyphotic deformity in AS.Methods:From June 2008 to January 2013,23 patients(18 males,5 females)at an average age of 44.8 years(range 25–69 years)were surgically treated for thoracolumbar kyphosis with ALs in AS via a modified posterior wedge osteotomy in our department.All sagittal balance parameters were assessed by standing lateral radiography of the whole spine before surgery and during the followup period.Assessment of radiologic fusion at follow-up was based on the Bridwell interbody fusion grading system.Ankylosing spondylitis quality of life(ASQoL)and visual analog scale(VAS)scores were performed to evaluate improvements in daily life function and back pain pre-operatively and post-operatively.Paired t tests were used to compare clinical data change in parametric values before and after surgery and the Mann-Whitney U test was employed for non-parametric comparisons.The radiographic data change was evaluated by repeated measure analysis of variance.Results:The mean operative duration was 205.4 min(range 115–375 min),with an average blood loss of 488.5 mL(range 215–880 mL).Radiographical and clinical outcomes were assessed after a mean of 61.4 months of follow-up.The VAS back pain and ASQoL scores improved significantly in all patients(7.52±1.31 vs.1.70±0.70,t=18.30,P<0.001;13.87±1.89 vs.7.22±1.24,t=18.53,P<0.001,respectively).The thoracolumbar kyphosis(TLK)changed from 40.03±17.61°pre-operatively to 13.86±6.65°post-operatively,and 28.45±6.63°at final follow-up(F=57.54,P<0.001),the thoracic kyphosis(TK)changed from 52.30±17.62°pre-operatively to 27.76±6.50°post-operatively,and 28.45±6.63°at final follow-up(F=57.29,P<0.001),and lumbar lordosis(LL)changed from29.56±9.73°pre-operatively to20.58±9.71°post-operatively,and20.73±10.27°at final follow-up(F=42.50,P<0.001).Mean sagittal vertical axis(SVA)was improved from 11.82±4.55 cm pre-operatively to 5.12±2.42 cm post-operatively,and 5.03±2.29 cm at final follow-up(F=79.36,P<0.001).No obvious loss of correction occurred,according to the lack of significant differences in the sagittal balance parameters between post-operatively and the final follow-up in all patients(TK:27.76±6.50°vs.28.45±6.63°,TLK:13.86±6.65°vs.14.42±6.7°,LL:20.58±9.71°vs.20.73±10.27°,and SVA:5.12±2.42 cm vs.5.03±2.29 cm,all P>0.05,respectively).Conclusions:The modified posterior wedge osteotomy is an accepted surgical procedure for treating thoracolumbar kyphosis with ALs in AS and results in satisfactory local kyphosis correction,solid fusion,and good clinical outcomes. | Hong-Yu Wei Chun-Ke Dong Yu-Ting Zhu Jun Zhou Ping Yi Feng Yang Ming-Sheng Tan | 2020 | Chinese Medical Journal2020，，2： | 5 |
| 12 | Back-n white neutron source at CSNS and its applications显示文摘Back-streaming neutrons from the spallation target of the China Spallation Neutron Source(CSNS)that emit through the incoming proton channel were exploited to build a white neutron beam facility(the so-called Back-n white neutron source),which was completed in March 2018.The Back-n neutron beam is very intense,at approximately 29107 n/cm2/s at 55 m from the target,and has a nominal proton beam with a power of 100 kW in the CSNS-I phase and a kinetic energy of 1.6 GeV and a thick tungsten target in multiple slices with modest moderation from the cooling water through the slices.In addition,the excellent energy spectrum spanning from 0.5 eV to 200 MeV,and a good time resolution related tothe time-of-flight measurements make it a typical white neutron source for nuclear data measurements;its overall performance is among that of the best white neutron sources in the world.Equipped with advanced spectrometers,detectors,and application utilities,the Back-n facility can serve wide applications,with a focus on neutron-induced cross-sectional measurements.This article presents an overview of the neutron beam characteristics,the experimental setups,and the ongoing applications at Backn. | Jing-Yu Tang Qi An Jiang-Bo Bai Jie Bao Yu Bao Ping Cao Hao-Lei Chen Qi-Ping Chen Yong-Hao Chen Zhen Chen Zeng-Qi Cui Rui-Rui Fan Chang-Qing Feng Ke-Qing Gao Xiao-Long Gao Min-Hao Gu Chang-Cai Han Zi-Jie Han Guo-Zhu He Yong-Cheng He Yang Hong Yi-Wei Hu Han-Xiong Huang Xi-Ru Huang Hao-Yu Jiang Wei Jiang Zhi-Jie Jiang Han-Tao Jing Ling Kang Bo Li Chao Li Jia-Wen Li Qiang Li Xiao Li Yang Li Jie Liu Rong Liu Shu-Bin Liu Xing-Yan Liu Ze Long Guang-Yuan Luan Chang-Jun Ning Meng-Chen Niu Bin-Bin Qi Jie Ren Zhi-Zhou Ren Xi-Chao Ruan Zhao-Hui Song Kang Sun Zhi-Jia Sun Zhi-Xin Tan Xin-Yi Tang Bin-Bin Tian Li-Jiao Wang Peng-Cheng Wang Zhao-Hui Wang Zhong-Wei Wen Xiao-Guang Wu Xuan Wu Li-Kun Xie Xiao-Yun Yang Yi-Wei Yang Han Yi Li Yu Tao Yu Yong-Ji Yu Guo-Hui Zhang Lin-Hao Zhang Qi-Wei Zhang Xian-Peng Zhang Yu-Liang Zhang Zhi-Yong Zhang Lu-Ping Zhou Zhi-Hao Zhou Ke-Jun Zhu 无 | 2021 | Nuclear Science and Techniques2021，32，1： | 4 |
| 13 | Rapamycin Sensitizes Glucocorticoid Resistant Acute Lymphoblastic Leukemia CEM-C1 Cells to Dexamethasone Induced Apoptosis through both mTOR Suppression and Up-Regulation and Activation of Glucocorticoid Receptor显示文摘Objective To explore the role of glucocorticoid (GC) receptor (GR) in rapamycin's reversion of GC resistance in humanGC-resistant T-acute lymphoblastic leukemia (ALL) CEM-C1 cells. Methods CEM-C1 cells were cultured in vitro and treated with rapamycin at different concentrations with or without 1 μmol/L dexamethasone (Dex). 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) test was performed to assess cell proliferation. The cell cycle and cell apoptosis were analyzed by flow cytometry. The expression of GRα mRNA was determined by real-time quantitative RT-PCR. The expression of GR, p-70S6K, Mcl-1, and Bim proteins was detected by Western blot. Results When incubated with rapamycin at different concentrations, CEM-C1 cells showed significant growth inhibition in a time- and concentration-dependent manner. The growth inhibition was synergistically increased when CEM-C1 cells were treated with rapamycin plus 1 μmol/L Dex. CEM-C1 cells treated with rapamycin alone showed no apparent apoptosis, and were arrested at G0/G1 phase. After the treatment with Dex plus rapamycin, CEM-C1 cells demonstrated apparent apoptosis and increased the cell cycle arrested at G0/G1 phase. Rapamycin combined with Dex up-regulated GRα, phosphorylated GR(p-GR), and pro-apoptotic protein Bim-EL in CEM-C1 cells, but inhibited the expression of p-p70S6K, a downstream target protein ofmTOR (mammalian target of rapamycin). Conclusion After the treatment with rapamycin plus Dex, Dex resistant CEM-C1 cells induce growth inhibition and apoptosis. The underlying mechanism may involve inhibition of the mTOR signaling pathway and also be associated with up-regulation of GR expression and activation of GC-GR signaling pathway. | GUO Xia ZHOU Chen Yan LI Qiang GAO Ju ZHU Yi Ping GU Ling MA Zhi Gui | 2013 | Biomedical and Environmental Sciences2013，26，5： | 4 |
| 14 | Novel Association of Killer Cell Immunoglobulin-like Receptor Genes with EBV-infectious Diseases in Children显示文摘Killer cell immunoglobulin-like receptors(KIRs)which are mainly expressed on natural killer(NK)cells are implicated in many virus infections.However,it is unclear whether or not KIRs are associated with susceptibility to Epstein-Barr virus(EBV)infection related diseases.Therefore,the purpose of our study was to investigate possible correlation between polymorphisms of KIR genes and infectious mononucleosis(IM)/EBV-associated hemophagocytic lymphohistiocytosis(EBV-HLH). | HUO Li JIANG Ming Yan LI Qiang ZHU Yi Ping | 2015 | Biomedical and Environmental Sciences2015，28，4： | 4 |
| 15 | Hepatitis B Immunoprophylactic Failure and Characteristics of the Hepatitis B Virus Gene in Mother-Infant Pairs in Parts of China显示文摘Objective To determine the hepatitis B immunoprophylactic failure rate in infants born to hepatitis B virus(HBV) infected mothers and to characterize HBV genes. Methods HBV-serological testing was conducted for pregnant women and infants. The complete genomes of 30 HBV isolates were sequenced, and genetic characteristics were analyzed using MEGA 5 software. Results The immunoprophylactic failure rate for infants who had completed the scheduled hepatitis B vaccination program was 5.76%(32/556). High sequence homology(99.8%-100%) was observed in 8 of the 10 mother-infant pairs. We identified 19 subgenotype C2 strains, 9 subgenotype B2 strains, and 2 subgenotype C1 strains. Three serotypes were detected: adr(19/30), adw(9/30), and ayw(2/30). The frequency of amino acid mutation of the ‘a' determinant region was 16.67%(5/30), including that of Q129 H, F134 Y, S136 Y, and G145 E. We detected 67 amino acid mutations in the basal core promoter, precore, and core regions of the genome. Conclusion The immunoprophylactic failure rate in infants born to HBV-infected mothers is low in the regions of China examined during this study. Moreover, HBV mutation in the ‘a' determinant region could not account for immunoprophylactic failure for all infants. | YIN Wen Jiao SHEN Li Ping WANG Fu Zhen ZHANG Guo Min ZHENG Hui WANG Feng LIU Tie Zhu MENG Qing Ling YI Yao CUI Fu Qiang BI Sheng Li | 2016 | Biomedical and Environmental Sciences2016，29，11： | 3 |
| 16 | Combined detection and subclass characteristics analysis of CTCs and CTECs by SE-iFISH in ovarian cancer显示文摘Objective:Hematogenous metastasis is essential for the progression of ovarian cancer(OC),and circulating tumor cells(CTCs)are part of the metastatic cascade.However,the detection rate of CTC is low due to the use of less sensitive detection methods.Therefore,this study aimed to detect CTCs and circulating tumorigenic endothelial cells(CTECs)in patients with OC using subtraction enrichment and immunostaining and fluorescence in situ hybridization(SE-iFISH).Methods:We enrolled a total of 56 subjects,including 20 OC patients and 36 ovarian benign tumor patients.CTCs and CTECs were captured by subtraction enrichment(SE)and counted and classified according to immunofluorescence staining of tumor markers(TMs)carbohydrate antigen 125(CA125)and human epididymis protein 4(HE4)combined with fluorescence in situ hybridization(iFISH)of chromosome 8(Chr8)aneuploidy.The diagnostic value and subtype characteristics of CTCs and CTECs were investigated.Results:The detection rate of CTCs by SE-iFISH was high.Compared with CA125 and HE4,Chr8 aneuploidy was the major identification feature of CTC.CTC counts in OC were statistically higher than those in benign groups.CTC and CTEC with≥pentaploidy were detected in both groups,illustrating the poor diagnostic value of CTC or CTEC.Distributions of triploid and tetraploid CTC subtypes were significantly different,and combined detection of triploid and tetraploid CTCs showed the best diagnostic value.In contrast,the distribution of CTECs in the OC and benign groups had no statistically significant difference.Small CTCs accounted for over 1/3 of the total CTC count.We also found that small CTCs and CTECs primarily comprised triploid cells,while large CTCs and CTECs mainly comprised pentaploidy and beyond.Conclusions:The application of SE-iFISH offered a more comprehensive understanding of heterogeneous CTCs and CTECs in OC.Analysis of subclass characteristics of the CTCs and CTECs according to Chr8 aneuploidy and cell size may broaden their potential clinical utility and deepen mechanistic studies in OC. | Hongyan Cheng Shang Wang Wenqing Luan Xue Ye Sha Dou Zhijian Tang Honglan Zhu Peter Ping Lin Yi Li Heng Cui Xiaohong Chang | 2021 | Chinese Journal of Cancer Research2021，33，2： | 3 |
| 17 | Three-dimensional visualization of the functional fascicular groups of a long-segment peripheral nerve显示文摘The three-dimensional(3D) visualization of the functional bundles in the peripheral nerve provides direct and detailed intraneural spatial information. It is useful for selecting suitable surgical methods to repair nerve defects and in optimizing the construction of tissue-engineered nerve grafts. However, there remain major technical hurdles in obtaining, registering and interpreting 2D images, as well as in establishing 3D models. Moreover, the 3D models are plagued by poor accuracy and lack of detail and cannot completely reflect the stereoscopic microstructure inside the nerve. To explore and help resolve these key technical problems of 3D reconstruction, in the present study, we designed a novel method based on re-imaging techniques and computer image layer processing technology. A 20-cm ulnar nerve segment from the upper arm of a fresh adult cadaver was used for acetylcholinesterase(ACh E) staining. Then, 2D panoramic images were obtained before and after ACh E staining under the stereomicroscope. Using layer processing techniques in Photoshop, a space transformation method was used to fulfill automatic registration. The contours were outlined, and the 3D rendering of functional fascicular groups in the long-segment ulnar nerve was performed with Amira 4.1 software. The re-imaging technique based on layer processing in Photoshop produced an image that was detailed and accurate. The merging of images was accurate, and the whole procedure was simple and fast. The least square support vector machine was accurate, with an error rate of only 8.25%. The 3D reconstruction directly revealed changes in the fusion of different nerve functional fascicular groups. In conclusion. The technique is fast with satisfactory visual reconstruction. | Jian Qi Wei-Ya Wang Ying-Chun Zhong Jia-Ming Zhou Peng Luo Ping Tang Cai-Feng He Shuang Zhu Xiao-Lin Liu Yi Zhang | 2018 | Neural Regeneration Research2018，13，8： | 3 |
| 18 | Cholesterol homeostasis regulated by ABCA1 is critical for retinal ganglion cell survival显示文摘Genome-wide association studies have suggested a link between primary open-angle glaucoma and the function of ABCA1.ABCA1 is a key regulator of cholesterol efflux and the biogenesis of high-density lipoprotein(HDL) particles. Here, we showed that the POAG risk allele near ABCA1 attenuated ABCA1 expression in cultured cells. Consistently, POAG patients exhibited lower ABCA1 expression, reduced HDL, and higher cholesterol in white blood cells. Ablation of Abca1 in mice failed to form HDL, leading to elevated cholesterol levels in the retina. Counting retinal ganglion cells(RGCs) by using an artificial intelligence(AI) program revealed that Abca1-deficient mice progressively lost RGCs with age. Single-cell RNA sequencing(scRNA-seq) revealed aberrant oxidative phosphorylation in the Abca1-/-retina, as well as activation of the mTORC1 signaling pathway and suppression of autophagy. Treatment of Abca1-/-mice using atorvastatin reduced the cholesterol level in the retina,thereby improving metabolism and protecting RGCs from death. Collectively, we show that lower ABCA1 expression and lower HDL are risk factors for POAG. Accumulated cholesterol in the Abca1-/-retina causes profound aberrant metabolism, leading to a POAG-like phenotype that can be prevented by atorvastatin. Our findings establish statin use as a preventive treatment for POAG associated with lower ABCA1 expression. | Jialiang Yang Yuhong Chen Tongdan Zou Bai Xue Fang Yang Xiangzhou Wang Yibo Huo Boyun Yan Yuxia Xu Shiyu He Yi Yin Jing Wang Xiong Zhu Lin Zhang Yu Zhou Zhengfu Tai Ping Shuai Man Yu Qian Luo Yilian Cheng Bo Gong Xianjun Zhu Jing Zhang Xinghuai Sun Ying Lin Houbin Zhang Zhenglin Yang | 2023 | Science China(Life Sciences)2023，66，2： | 2 |
| 19 | Identification and comparison of micro RNA s from skeletal muscle and adipose tissues from two porcine breeds显示文摘 | Hong‐Yi Li Qian‐Yun Xi Yuan‐Yan Xiong Xiao‐Long Liu Xiao Cheng Gang Shu Song‐Bo Wang Li‐Na Wang Ping Gao Xiao‐Tong Zhu Qing‐Yan Jiang Li Yuan Yong‐Liang Zhang | 2012 | Anim Genet2012，，6： | 2 |
| 20 | Construction and prokaryotic expression of the fusion protein Stx2B-IntiminC300 of EHEC O157: H7 and its immunoprophylactic potential显示文摘To construct and express the fusion protein Stx2B-IntiminC300 of EHEC 0157 :H7, and to further investigate its immunoprophyiactic potential, the gene of Stx2B (stx2b) from EHEC 0157:H7 chromosome was cloned into pMD18-T vector. Thereafter, the amplified gene was cloned into prokaryotic expression plasmid pET-28a (+)-eaeC300, which was constructed previously. The recombinant pasmid pET-28a(+)-stx2b-eaeC300 was transformed into E. coli BL21(DE3). After inducement, the protein Stx2B-IntiminC300 was successfully expressed and analyzed with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting and N-terminal amino acid residual sequencing. To evaluate its immunoprophyiactic potential, it was primarily purified by ion-exchange chromatography and injected into 30 BALB/c mice with Al(OH)3 in the subscapular region. Ten days after the last booster vaccination, 20 mice were attacked with EHEC 0157: H7 lysate and the protective efficacy was observed. In the present study, the gene of Stx2B-IntiminC300 was successfully cloned into pET-28a (+) vector. The results of SDS-PAGE and Western blotting assay showed that the fusion protein was successfully expressed in the inclusion body form, accounting for 25% of total expression products, and its molecular weight was about 43 kDa. The result of the N-terminal amino acid residual sequencing showed that it was identical to that of the molecular designed. The purity was about 75 % after primary purification. Animal tests revealed that the fusion protein Stx2B-IntiminC300 has elicited high titer of protective antibody relatively. These results demonstrate that the fusion protein Stx2B-IntiminC300 is successfully expressed in prokaryotic expression system and shows certain immunoprophyiactic potential. | YONG YI Xu Hu MAO WEN DE TONG YING MA MING ZEN YONG HONG ZHU QUAN MING ZOU XIA AI JIAN PING CHENG WEI JUN ZHANG JIANG GU PING LUO | 2006 | Journal of Microbiology and Immunology2006，4，2： | 2 |
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