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| 1 | Heterologous expression of human cytochrome P450 2E1 in HepG2 cell line显示文摘AIM: Human cytochrome P-450 2E1 (CYP2E1) takes part in the biotransformation of ethanol, acetone, many smallmolecule substrates and volatile anesthetics. CYP2E1 is involved in chemical activation of many carcinogens,procarcinogens, and toxicants. To assess the metabolic and toxicological characteristics of CYP2E1, we cloned CYP2E1 cDNA and established a HepG2 cell line stably expressing recombinant CYP 2E1.METHODS: Human CYP2E1 cDNA was amplified with reverse transcription-polymerase chain reaction (RT-PCR)from total RNAs extracted from human liver and cloned into pGEM-T vector. The cDNA segment was identified by DNA sequencing and subcloned into a mammalian expression vector pREP9. A transgenic cell line was established by transfecting the recombinant plasmid of pREP9-CYP2E1 to HepG2 cells. The expression of CYP2E1 mRNA was validated by RT-PCR. The enzyme activity of CYP2E1 catalyzing oxidation of 4-nitrophenol in postmitochondrial supernate (S9) fraction of the cells was determined by spectrophotometry. The metabolic activation of HepG2-CYP2E1 cells was assayed by N-nitrosodiethylamine (NDEA)cytotoxicity and micronucleus test.RESULTS: The cloned CYP2E1 cDNA segment was identical to that reported by Umeno et al(GenBank access No.J02843). HepG2-CYP2E1 cells expressed CYP2E1 mRNA and had 4-nitrophenol hydroxylase activity (0.162±0.025nmol.min-1.mg-1 S9 protein), which were undetectable in parent HepG2 cells. HepG2-CYP2E1 cells increased the cytotoxicity and micronucleus rate of NDEA in comparison with those of HepG2 cells.CONCLUSION: The cDNA of human CYP2E1 can be successfully cloned, and a cell line, HepG2-CYP2E1, which can efficiently express mRNA and has CYP2E1 activity, is established. The cell line is useful for testing the cytotoxicity,mutagenicity and metabolism of xenobiotics, which may possibly be activated or metabolized by CYP2E1. | Jian Zhuge Ye Luo Ying-Nian Yu Department of Pathophysiology,School of Medicine,Zhejiang University,Hangzhou 310031,Zhejiang Province,China | 2003 | World Journal of Gastroenterology2003，9，12： | 7 |
| 2 | Validation of the Nanjing Criteria for Diagnosing Pyrrolizidine Alkaloids-induced Hepatic Sinusoidal Obstruction Syndrome显示文摘Background and Aims:Hepatic sinusoidal obstruction syndrome(HSOS)is caused by toxic injury to sinusoidal endothelial cells in the liver.The intake of pyrrolizidine alkaloids(PAs)in some Chinese herbal remedies/plants remains the major etiology for HSOS in China.Recently,new diagnostic criteria for PA-induced HSOS(i.e.PA-HSOS)have been developed;however,the efficacy has not been clinically validated.This study aimed to assess the performance of the Nanjing criteria for PA-HSOS.Methods:Data obtained from consecutive patients in multiple hospitals,which included 86 PA-HSOS patients and 327 patients with other liver diseases,were retrospectively analyzed.Then,the diagnostic performance of the Nanjing criteria and simplified Nanjing criteria were evaluated and validated.The study is registered in www.chictr.org.cn(ID:ChiCTR1900020784).Results:The Nanjing criteria have a sensitivity and specificity of 95.35%and 100%,respectively,while the simplified Nanjing criteria have a sensitivity and specificity of 96.51%and 96.33%,respectively,for the diagnosis of PA-HSOS.Notably,a proportion of patients with Budd-Chiari syndrome(11/49)was misdiagnosed as PA-HSOS on the basis of the simplified Nanjing criteria,and this was mainly due to the overlapping features in the enhanced computed tomography/magnetic resonance imaging examinations.Furthermore,most of these patients(10/11)had occlusion or thrombosis of the hepatic vein,and communicating vessels in the liver were found in 8/11 patients,which were absent in PA-HSOS patients.Conclusions:The Nanjing criteria and simplified Nanjing criteria exhibit excellent performance in diagnosing PA-HSOS.Thus,both could be valuable diagnostic tools in clinical practice. | Wei Zhang Lu Liu Ming Zhang Feng Zhang Chunyan Peng Bin Zhang Jun Chen Lin Li Jian He Jiangqiang Xiao Yanhong Feng Xunjiang Wang Aizhen Xiong Li Yang Xiaoping Zou Yuecheng Yu Yuzheng Zhuge | 2021 | Journal of Clinical and Translational Hepatology2021，9，3： | 4 |
| 3 | Inhibition of the mitoehondrial permeability transition by eyeclosporin Aprevents pyrazole plus lipopolysaeeharide-indueed liver injury in miee显示文摘 | Jian Zhuge Arthur l Cederbaum | 2009 | Free Radical Biology & Medicine2009，46，3： | 1 |
| 4 | Biochemical properties of a thermostable phytase from Neurospora crassa显示文摘 | Zhou Xiao-ling Wei Shen Zhuge Jian Wang Zheng-xiang | 2006 | FEMS Microbiol Lett2006，258，： | 1 |
| 5 | Glycerol production by a novel osmotolerant yeast Candida glycerinogenes显示文摘 | Zhuge Jian FANG Hui-ying WANG Zheng-xiang | 2001 | Applied and Microbiol Biotechnology2001，55，： | 1 |
| 6 | Glycerol production by microbial fermentation:a review显示文摘 | Wang Zheng-xiang Zhuge Jian Prior BA | 2001 | Biotechnology Advances2001，19，： | 1 |
| 7 | Cloning and over-expression of an alkaline protease from Bacillus licheniformis显示文摘 | Xue-Ming Tang Wei Shen F.M. Lakay Wei-Lan Shao Zheng-Xiang Wang B.A. Prior Jian Zhuge | 2004 | Biotechnology Letters2004，，12： | 1 |
| 8 | Expression of a Bacillus subtilis pectate lyase gene in Pichia pastoris显示文摘 | Bin Zhuge Guo-Cheng Du Wei Shen Jian Zhuge Jian Chen | 2007 | Biochemical Engineering Journal2007，，1： | 1 |
| 9 | Isolation and sequence analysis of the gene Ura3 encoding the orotidione-5'-phosphate decarboxylase from candida glucerinogenes WL2002-5 an industrial glycerol producer显示文摘 | Li Yanli Shen Wei Zhuge Jian | 2005 | Yeast2005，22，6： | 1 |
| 10 | Glycerol production by a novel osmotolerant yeast Candida glyc- erinogenes显示文摘 | ZHUGE Jian FANG Huiying WANG Zhenxiang | 2001 | Appl Microbiol Biotechnol2001，55，： | 1 |
| 11 | Glycerol production by microbial fermentation: A review显示文摘 | Wang Zheng-Xiang Zhuge Jian Fang Huiying | 2001 | Biotechnology Advances2001，19，： | 1 |
| 12 | Glycerol production by a novel osmotolerant yeast Candida glycerolgenesis显示文摘 | ZHUGE Jian FANG Hui-ying WANG Zeng xiang | 2001 | Applied Microbiology and Biotechnology2001，55，6： | 1 |
| 13 | By-product formation by a novel glycerol-producing yeast, Candida glyccrinogencs, with different O2 supplies显示文摘 | Hairu Jin Huiying Fang Jian Zhuge | 2003 | Biotechnology Letters2003，，25： | 1 |
| 14 | Glycerol production by microbial fermentation: a review显示文摘 | WANG Zhengxiang ZHUGE Jian FANG Huiying | 2001 | Biotechnology Advances2001，19，： | 1 |
| 15 | Glycerol production by a novel osmotolerant yeast Candida glycerinogenes 显示文摘 | Zhuge Jian Fang Huiying Wang Zhengxiang | 2001 | Appllied Microbiology and Biotechnology2001，55，： | 1 |
| 16 | By-product formation by a novel glycerol-producing Yeast, Candida glycerinogenes, with different O2 supplies 显示文摘 | Jin Hairong Fang Huiying Zhuge Jian | 2003 | Biotechnology Letters2003，25，： | 1 |
| 17 | Glycerol producing by microbial fermentation : a review 显示文摘 | Wang Zhengxiang Zhuge Jian Prior BA | 2001 | Biotechnology Advance2001，19，： | 1 |
| 18 | Amphiphilic ABA Triblock Coploymer as Surfactant in Syntheses of Microlatexes Bearing Cationic Groups显示文摘 | Pei- Hong Ni Ming- Zu Zhang Lan- Jian Zhuge Shou - Kuan Fu | 2002 | Journal of Polymer Science : Part A: Polymer Chemistry2002，，40： | 1 |
| 19 | Expert Consensus on Assessment of Rupture Factors in Unruptured Intracranial Aneurysms显示文摘Intracranial aneurysms have a high prevalence in the population and are associated with high rates of disability and mortality after rupture.It is essential to assess the rupture risk of unruptured intracranial aneurysms by selecting appropriate morphological parameters.However,there is still a need for consistent evidence-based medicine to provide a clinical reference.Therefore,the expert group has jointly written this consensus based on literature review and clinical experience,aiming to provide a standardized reference for predicting the development of intracranial aneurysms and evaluating their rupture risk factors. | Ren-Ya Zhan Jian-Wei Pan Jian Shen Yu Zhu Jie Shen Fan Wu Hong-Wei He Hua Yang Jing Dai Xiang-Yu Wang Jun Ren Ying Xia Wei Wang Li-Qun Jiao Chuan-Lu Jiang Qing Lan Yi-Rong Wang Qi-Chuan Zhuge Guo-Hua Mao Hua Lu Xin-Gen Zhu Wen-Hua Yu Yong Jing Xiang-Dong Zhu | 2022 | Journal of Cerebrovascular Disease2022，5，3： | 0 |
| 20 | Bioinformatics Analysis and Identification of Key Candidate Genes Associated with Nephrotoxicity Induced by Cobalt显示文摘Cobalt(Co)is a silver-gray,high-intensity,widely distributed metal element that exists in cobalt compounds,and its common valences are bivalence(Co2+)and trivalence(Co3+)[1].The main routes of Co-exposure are occupational and environmental exposures.The human body can be exposed to high concentrations of Co2+through inhalation of contaminated air,consumption of contaminated food and water,or ingestion of Co-containing supplements[2]. | WANG Rui ZHANG Ding ZHUGE Rui Jian XUE Qian HE Chang Hao MA Wen Xuan SHEN Zhu Bin GUO Li | 2023 | Biomedical and Environmental Sciences2023，36，2： | 0 |
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