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| 1 | Establishing eutrophication assessment standards for four lake regions, China显示文摘The trophic status assessment of lakes in diferent lake regions may provide important and fundamental information for lake trophic state classification and eutrophication control. In this study, a region-specific lake eutrophication assessment standard was established through a frequency distribution method based on chlorophyll-a concentration. The assessment standards under the oligotrophic state for lakes in the Eastern plain, Yungui Plateau, Northeast Plain and Mountain Mongolia-Xinjiang regions are total phosphorus of 0.068,0.005, 0.011, 0.005 mg/L; total nitrogen of 1.00, 0.16, 0.37, 0.60 mg/L; Secchi depth of 0.60, 8.00, 1.55, 3.00 m; and CODMn of2.24, 1.00, 5.11, 4.00 mg/L, respectively. Moreover, a region-specific comprehensive trophic level index was developed to provide an understandable assessment method for the public. The results indicated that the frequency distribution analysis based on chlorophyll-a combined with trophic level index provided a useful metric for the assessment of the lake trophic status. In addition, the diference of eutrophication assessment standards in diferent lake regions was analyzed, which suggested that the sensitivities of algae to nutrients and the assessment standard of trophic status possessed significant regional diferences for the four lake ecoregions. Lake eutrophication assessment standards would contribute to maximizing the efectiveness of future management strategies, to control and minimize lake eutrophication problems. | Shouliang Huo Chunzi Ma Beidou Xi Jing Su Fengyu Zan Danfeng Ji Zhuoshi He | 2013 | Journal of Environmental Sciences2013，25，10： | 18 |
| 2 | Bufalin suppresses the migration and invasion of prostate cancer cells through HOTAIR, the sponge of miR-520b显示文摘Bufalin, the major active component of the traditional Chinese medicine ChanSu obtained from the skin and parotid venom glands of toads, has long been known as an anticancer agent. Recent studies show that microRNAs (miRs) are involved in the anticancer activities of bufalin, while long non-coding RNAs (lncRNAs) are known to interact with miRNAs to regulate various biological functions. In this paper, we investigated the possible network related to the antimetastatic effect of bufalin in prostate cancer (PCa) cells. We demonstrated that bufalin (0.05?10 μM) dose-dependently suppressed the proliferation of prostate cancer DU145 and PC3 cells with IC50 values of 0.89 and 1.28 μM, respectively. Furthermore, bufalin treatment significantly suppressed the cell migration and invasion. To explore the role of lncRNAs in the antimetastatic activity of bufalin, we used an lncRNA microarray and found that HOX transcript antisense RNA (HOTAIR) was the most markedly downregulated lncRNA in bufalin-treated PCa cells. Overexpression of HOTAIR counteracted the suppressing effects of bufalin on DU145 and PC3 cells. We then predicted and verified that HOTAIR upregulated FGFR1 expression by sponging miR-520b in PCa cells. In 40 patients with PCa bone metastasis, we used in situ hybridization or immunohistochemical assay to assess the HOTAIR and FGFR1 expression, which revealed that both HOTAIR and FGFR1 expression were significantly higher in bone metastasis tissues than in the primary PCa tissues. In addition, the level of serum HOTAIR was positively associated with the levels of serum bone metabolic markers (CTx, OST, B-ALP and PINP) and may serve as a reasonable biomarker for PCa bone metastasis. Taken together, this is the first study revealing that HOTAIR promotes PCa bone metastasis, and bufalin may be a promising candidate for the treatment of this disease. | Jian-jun Zhang Xiao-hui Zhou Yan Zhou Yong-gang Wang Bin-zhi Qian Ai-na He Zan Shen Hai-yan Hu Yang Yao | 2019 | Acta Pharmacologica Sinica2019，40，9： | 13 |
| 3 | Potato/Maize intercropping reduces infestation of potato tuber moth, Phthorimaea operculella(Zeller) by the enhancement of natural enemies显示文摘The potato tuber moth(PTM),Phthorimaea operculella(Zeller),is one of the most economically significant insect pests for potato in both field and storage worldwide.To evaluate the infestation,reduction of potato yield and the control efficacy for PTM,field tests were conducted in two seasons by intercropping of potato as the host plant with maize as a non-host plant of PTM.Three intercropping patterns were tested,which were 2 rows of potatoes with either 2,3,or 4 rows of maize(abbreviated 2 P:2 M,2 P:3 M,and 2 P:4 M),and the monocropped potato as the control,2 rows of potatoes,without maize,(abbreviated 2 P:0 M).Results showed that the population and infestation of PTM in the 2 P:3 M intercropping pattern was significantly lower than those in 2 P:2 M,2 P:4 M and the monocropping pattern of 2 P:0 M,due to the enhancement of natural enemies.Cumulative mines and tunneling in potato leaves in 2 P:3 M intercropping were significantly lower than those in 2 P:2 M and 2 P:4 M patterns.The population of parasitoids and the parasitism rate of PTM in intercropping pattern of 2 P:3 M were significantly higher than that in intercropping pattern of 2 P:2 M,2 P:4 M and monocropping pattern of 2 P:0 M.We conclude that the potato intercropped with maize reduced the adult and larva populations,and reduced the damage from PTM by enhancing the number of parasitoids and the level of parasitism.The greatest population density of parasitoids and parasitism rate were in the intercropping pattern of 2 rows of potatoes with 3 rows of maize.These data indicate that the host/non-host intercropping patterns can be used as a biological control tactic against PTM by enhancing the density of natural enemies in the agro-ecosystems. | ZHENG Ya-qiang ZHANG Li-min CHEN Bin YAN Nai-sheng GUI Fu-rong ZAN Qing-an DU Guang-zu HE Shu-qi LI Zheng-yue GAO Yu-lin XIAO Guan-li | 2020 | Journal of Integrative Agriculture2020，19，2： | 8 |
| 4 | Existence of Solutions of Boundary Value Problem Differential a Nonlinear Three-Point for Third-Order Ordinary Equations显示文摘In this paper,existence of solutions of third-order differential equation ■with nonlinear three-point boundary condition ■is obtained by embedding Leray-Schauder degree theory in upper and lower solutions method,where a,b,c ∈ R,a < b < c;f :[a,c]×R3 → R,g :R3 → R,h :R2 → R and I :R3 → R are continuous functions.The existence result is obtained by defining the suitable upper and lower solutions and introducing an appropriate auxiliary boundary value problem.As an application,an example with an explicit solution is given to demonstrate the validity of the results in this paper. | SHEN Jian He ZHOU Zhe Yan YU Zan Pin | 2009 | Journal of Mathematical Research and Exposition2009，29，1： | 6 |
| 5 | Progress in green and efficient enrichment of rare earth from leaching liquor of ion adsorption type rare earth ores显示文摘Ion adsorption type rare earth ores(IATREOs)are a valuable strategic mineral resource in China,which feature a complete composition of fifteen rare earth elements and are rich in medium and heavy rare earth(RE)elements.In the leaching process for recovering rare earth elements from IATREOs,many impurities will be leached together with rare earth elements and enter the leaching liquor.An impurity removal-precipitation enrichment technique is currently applied to selectively recovery rare earth elements from the leaching liquor with the high content of impurities and low concentration of rare earth elements by using ammonium bicarbonate in the industry.However,a high loss of rare earth elements and severe ammonia nitrogen pollution are caused by this process.Therefore,more beneficial impurities removal technologies,mainly for aluminum,and green enrichment technologies with lower pollution are now urgently needed.For this purpose,this paper analyzed two aspects of research progress in recent decades:the green separation of rare earth elements and aluminum from leaching liquor and the green and efficient enrichment of rare earth elements.Finally,an approach for the high-efficiency and green enrichment of rare earth elements from leaching liquor of the IATREOs is proposed in several aspects,including impurity inhibition leaching,neutralization and impurity removal,alkaline calcium and magnesium salt precipitation enrichment,and centrifugal extraction enrichment. | Qiang He Jiang Qiu Jinfa Chen Miaomiao Zan Yanfei Xiao | 2022 | Journal of Rare Earths2022，40，3： | 5 |
| 6 | An injectable and self-healing hydrogel with controlled release of curcumin to repair spinal cord injury显示文摘The harsh local micro-environment following spinal cord injury(SCI)remains a great challenge for neural regeneration.Local reconstitution of a favorable micro-environment by biocompatible scaffolds with desirable functions has thus been an area of concern.Herein,a hybrid hydrogel was developed using Fmoc-grafted chitosan(FC)and Fmoc peptide(FI).Dynamic reversibleπ-πstacking interactions of the fluorenyl rings enabled the FC/FI hybrid hydrogel to exhibit excellent injectable and self-healing properties,as characterized by visual appearances and rheological tests.Furthermore,the FC/FI hybrid hydrogel showed a slow and persistent release of curcumin(Cur),which was named as FC/FI-Cur hydrogel.In vitro studies confirmed that with the support of FC/FI-Cur hydrogel,neurite outgrowth was promoted,and Schwann cell(SC)migration away from dorsal root ganglia(DRG)spheres with enhanced myelination was substantiated.The FC/FI-Cur hydrogel well reassembled extracellular matrix at the lesion site of rat spinal cord and exerted outstanding effects in modulating local inflammatory reaction by regulating the phenotypes of infiltrated inflammatory cells.In addition,endogenous SCs were recruited in the FC/FI-Cur graft and participated in the remyelination process of the regenerated nerves.These outcomes favored functional recovery,as evidenced by improved hind limbs movement and enhanced electrophysiological properties.Thus,our study not only advanced the development of multifunctional hydrogels but also provided insights into comprehensive approaches for SCI repair. | Jinghua Luo Xueshuang Shi Liming Li Zan Tan Feng Feng Jun Li Mao Pang Xiaoying Wang Liumin He | 2021 | Bioactive Materials2021，6，12： | 4 |
| 7 | Viral kinetics of Enterovirus 71 in human habdomyosarcoma cells显示文摘AIM:To characterise the viral kinetics of enterovirus 71 (EV71).METHODS:In this study,human rhabdomyosarcoma (RD) cells were infected with EV71 at different multiplicity of infection (MOI).After infection,the cytopathic effect (CPE) was monitored and recorded using a phase contrast microscope associated with a CCD camera at different time points post viral infection (0,6,12,24 h post infection).Cell growth and viability were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in both EV71 infected and mock infected cells at each time point.EV71 replication kinet-ics in RD cells was determined by measuring the total intracellular viral RNA with real-time reverse-transcription polymerase chain reaction (qRT-PCR).Also,the intracellular and extracellular virion RNA was isolated and quantified at different time points to analyze the viral package and secretion.The expression of viral protein was determined by analyze the levels of viral structure protein VP1 with Western blotting.RESULTS:EV71 infection induced a significant CPE as early as 6 h post infection (p.i.) in both RD cells infected with high ratio of virus (MOI 10) and low ratio of virus (MOI 1).In EV71 infected cells,the cell growth was inhibited and the number of viable cells was rapidly decreased in the later phase of infection.EV71 virions were uncoated immediately after entry.The intracellular viral RNA began to increase at as early as 3 h p.i.and the exponential increase was found between 3 h to 6 h p.i.in both infected groups.For viral structure protein synthesis,results from western-blot showed that intracellular viral protein VP1 could not be detected until 6 h p.i.in the cells infected at either MOI 1 or MOI 10;and reached the peak at 9 h p.i.in the cells infected with EV71 at both MOI 1 and MOI 10.Simultaneously,the viral package and secretion were also actively processed as the virus underwent rapid replication.The viral package kinetics was comparable for both MOI 1 and MOI 10 infected groups.It was observed that at 3 h p.i,the intracellular virions obviously decreased,thereafter,the intracellular virions began to increase and enter into the exponential phase until 12 h p.i.The total amounts of intracellular virons were decreased from 12 to 24 h p.i.Consistent with this result,the increase of virus secretion occurred during 6 to 12 h p.i.CONCLUSION:The viral kinetics of EV71 were established by analyzing viral replication,package and secretion in RD cells. | Jing Lu Li-Na Yi Hsiang-Fu Kung Ming-Liang He Ya-Qing He Hong Zan | 2011 | World Journal of Gastroenterology2011，17，36： | 3 |
| 8 | Spinel-layered integrate structured nanorods with both high capacity and superior high-rate capability as cathode material for lithium-ion batteries显示文摘尖晶石阶段描述 2 O 4 成功地被嵌进单斜晶的阶段 layeredstructured 李 2 MnO 3 nanorods,和这些尖晶石分层集成结构化的 nanorods 为锂离子电池(解放) 作为阴极材料显示出两个高能力和优异高率的能力。太古的李 2 MnO 3 nanorods 被一个简单 rheological 阶段方法作为先锋用 -MnO2 nanowires 综合。尖晶石分层集成结构化的 nanorods 被灵巧的部分减小反应作为 reductant 用硬脂的酸制作。结构的描述和 integrate 的电气化学的性质组织了验证的 nanorods 描述 2 O 4 nanodomains 在太古的李 2 MnO 3 nanorods。当为解放用作阴极材料时,尖晶石分层集成结构化的李 2 MnO 3 nanorods (SL-Li 2 MnO 3) 比太古的分层结构的李 2 MnO 3 nanorods (L-Li2MnO3 ) 。当解除费用时在 20 mAommonly 存在的 i?? | Huibing He Hengjiang Cong Ya Sun Ling Zan Youxiang Zhang | 2017 | Nano Research2017，10，2： | 3 |
| 9 | Utilization of aluminum hydroxide waste generated in fluoride adsorption and coagulation processes for adsorptive removal of cadmium ion显示文摘尽管基于 Al 的凝结和吸附过程为氟化物(F) 移动被证明高度有效,二个过程两个都产生大量艾尔(哦)3 固体废物包含 F (艾尔(哦)3-F) 。这研究试图调查利用艾尔的可行性(哦) 在艾尔产生的 3-F (哦)3 吸附(艾尔(哦)3-F 广告 ) 和凝结(艾尔(哦)3-F 为镉离子(Cd (II )) 的吸附的 coag ) 。艾尔的吸附能力(哦)3-F 广告 和艾尔(哦)3-F 为 Cd (II ) 的 coag 作为太古的铝氢氧化物的类似(艾尔(哦) 具有 24.39 和 19.90 mg 映慬Р的 3), | Jiawei JU Ruiping LIU Zan HE Huijuan LIU Xiwang ZHANG Jiuhui QU | 2016 | Frontiers of Environmental Science & Engineering2016，10，3： | 2 |
| 10 | Validation of Reference Genes for Quantitative Real-Time PCR in Laodelphax striatellus显示文摘The normalization of quantitative real-time PCR(qPCR) is important to obtain accurate gene expression data, and the most common method for qPCR normalization is to use reference genes. However, reference genes can be regulated under different conditions. qPCR has recently been used for gene expression study in Laodelphax striatellus, but there is no study on validation of the reference genes. In this study, five new housekeeping genes(LstrTUB1, LstrTUB2, LstrTUB3, LstrARF and LstrRPL9) in L. striatellus were cloned and deposited in the GenBank with accession numbers of JF728809, JF728810, JF728811, JF728807 and JF728806, respectively. Furthermore, mRNA expressions of the five genes and β-actin were measured by qPCR with insect samples of different instar at nymph stage, and the expression stabilities were determined by the software geNorm and NormFinder. As a result, ARF and RPL9 were consistently more stable than β-actin, while three TUB genes were less stable than β-actin. To determine the optimal number of reference genes used in qPCR, a pairwise variations analysis by geNorm indicated that two references ARF and RPL9 were required to obtain the accurate quantification. These results were further confirmed by the validation qPCR experiment with chitinase gene as the target gene, in which the standard error of the mRNA quantification by using binary reference ARF-RPL9 was much lower than those by ARF, RPL9 or β-actin alone. Taken together, our study suggested that the combination of ARF-RPL9 could replace β-actin as the reference genes for qPCR in L. striatellus. | HE Xiu-ting LIU Cheng-cheng LI Zhao-qun ZHANG Zan LI Guo-qing LI Fei DONG Shuang-lin | 2014 | Journal of Integrative Agriculture2014，13，4： | 2 |
| 11 | Design and preliminary experimental research on a new biogas fermentation system by solar heat pipe heating显示文摘Biogas fermentation requires appropriate temperature,while the biogas fermentation can be affected by the low ambient temperature in winter.In order to overcome the negative effects of low temperature fermentation,a new type of solar heat pipe biogas fermentation heating system was designed and a preliminary experiment research on this system was conducted using cow manure as the raw material at 6%concentration and total fermentation volume of 175 L.The experimental results showed that when the system was in normal operation,the fermentation temperature rose every day by gradient.This gradient will gradually become smaller with the increase of fermentation liquid temperature,and the temperature can reach 38°C after stability.Using this solar heat pipe heating system,the fermentation liquid temperature can be increased by 5°C every sunny day.This solar heat pipe heating system plays a significant role in biogas fermentation.The results of economic analysis show that the system can realize the fermentation at constant temperatures of 25°C and 35°C respectively,and it can also save standard coal equivalent of 40 kg and 80 kg in winter and spring,respectively. | Jiao Youzhou Li Pengfei Li Gang Zhang Quanguo Ding Pan Wang Shaopeng Gao Zan Du Shuangli He Chao | 2016 | International Journal of Agricultural and Biological Engineering2016，9，2： | 2 |
| 12 | Dynamic behavior and fracture mode of TiAI intermetallics with different microstructures at elevated temperatures显示文摘 | Zan X He Y H Xia Y M | | 0，，1： | 1 |
| 13 | Preparation and evaluation of anti-SARS coronavirus IgY from yolks of immunized SPF chickens显示文摘 | Chao-Yang Fu He Huang Xiao-Mei Wang Yong-Gang Liu Zhi-Guo Wang Shang-Jin Cui Hong-Lei Gao Zan Li Jing-Peng Li Xian-Gang Kong | 2006 | Journal of Virological Methods2006，133，： | 1 |
| 14 | Dynamic behavior and fracture mode of TiA1 intermetallics with different microstructures at elevated temperatures 显示文摘 | ZAN X HE Y H WANG Y | 2011 | Trans Nonferrous Met Soc China2011，，21： | 1 |
| 15 | Tensile impact behavior and deformation mechanism of duplex TiA1 intermetallics at elevated temperatures 显示文摘 | ZAN X HE Y WANG Y LU Z | 2010 | Journal of Materials Science2010，45，23： | 1 |
| 16 | Med19 Promotes Osteosarcoma Growth and Metastasis and Associates with Prognosis显示文摘 | Wenxi Yu Zhang Zhichang Min Daliu Yang Qingcheng Du Xuefei Tang Lina Lin Feng Sun Yuanjue Zhao Hui Zheng Shuier He Aina Li Hongtao Yao Yang Zan Shen | 2014 | European Journal of Cancer2014，，： | 1 |
| 17 | Preparation and evaluation of a macroporous molecularly imprinted hybrid silica monolithic column for recognition of proteins by high performance liquid chromatography 显示文摘 | Lin Zan Yang Fan He Xiwen | 2009 | Journal of Chromatography A2009，16，12： | 1 |
| 18 | Modified graphite filled natural rubber composites with good thermal conductivity显示文摘The rubber composites with good thermal conductivity contribute to heat dissipation of tires. Graphite filled natural rubber composites were developed in this study to provide good thermal conductivity. Graphite was coated with polyacrylate polymerized by monomers including methyl methacrylate, n-butyl acrylate and acrylic acid. The ratios between a filler and acrylate polymerization emulsion and those between monomers were varied. Eight types of surface modification formulas were experimentally investigated. Modification formula can affect coating results and composite properties greatly. The best coating type was achieved by a ratio of1:1 between methyl methacrylate and n-butyl acrylate. The coating of graphite was thermally stable in a running tire. Filled with modified graphite, the tire thermal conductivity reached up to 0.517–0.569 W·m-1·K-1. In addition, the mechanical performance was improved with increased crosslink density, extended scorch time and short vulcanization time. | Junping Song Lianxiang Ma Yan He Haiquan Yan Zan Wu Wei Li | 2015 | Chinese Journal of Chemical Engineering2015，23，5： | 1 |
| 19 | RNA-seq analysis reveals the critical role of the novel lnc RNA BIANCR in intramuscular adipogenesis through the ERK1/2 signaling pathway显示文摘Background Long non-coding RNAs(lncRNAs)regulate numerous biological processes,including adipogenesis.Research on adipogenesis will assist in the treatment of human metabolic diseases and improve meat quality in livestock,such as the content of intramuscular fat(IMF).However,the significance of lncRNAs in intramuscular adipogenesis remains unclear.This research aimed to reveal the lncRNAs transcriptomic profiles in the process of bovine intramuscular adipogenesis and to identify the lncRNAs involved in the adipogenesis of bovine intramuscular adipocytes.Results In this research,a landscape of lncRNAs was identified with RNA-seq in bovine intramuscular adipocytes at four adipogenesis stages(0 d,3 d,6 d,and 9 d after differentiation).A total of 7035 lncRNAs were detected,including 3396 novel lncRNAs.Based on the results of differential analysis,co-expression analysis,and functional prediction,we focused on the bovine intramuscular adipogenesis-associated long non-coding RNA(BIANCR),a novel lncRNA that may have an important regulatory function.The knockdown of BIANCR inhibited proliferation and promoted apoptosis of intramuscular preadipocytes.Moreover,BIANCR knockdown inhibited intramuscular adipogenesis by regulating the ERK1/2 signaling pathway.Conclusion This study obtained the landscape of lncRNAs during adipogenesis in bovine intramuscular adipocytes.BIANCR plays a crucial role in adipogenesis through the ERK1/2 signaling pathway.The results are noteworthy for improving beef meat quality,molecular breeding,and metabolic disease research. | Xinhao Ma Xinran Yang Dianqi Zhang Wenzhen Zhang Xiaoyu Wang Kuncheng Xie Jie He Chugang Mei Linsen Zan | 2023 | Journal of Animal Science and Biotechnology2023，14，3： | 1 |
| 20 | Effects of Diet on the Volatile Flavor and Nutritional Ingredients of Common Octopus(Octopus vulgaris)显示文摘Cephalopods are important economic shellfish that have been developed extensively in the coastal water of various countries. Octopus vulgaris is a large-scaled economic cephalopod that is mainly cultured in South China. This study explored the effect of different diets on the volatile flavor and nutritional ingredients of O. vulgaris. Four diets were tested in four groups: Group A(fish(Scomberomorus niphonius)), Group B(crab(Helice tridens tientsinensis Rathbun)), Group C(clam(Mactra veneriformis)), and Group D(squid(Loligo japonica)). Octopus muscles were sampled after 36 days of feeding, and volatile flavor substances(VFSs), fatty acids(FAs), and amino acids(AAs) were detected. Results showed that the VFSs, FAs, and AAs of octopus in the four groups were obviously different. The sum of volatile ketones and aldehydes was higher in Group B than in the other groups, which could present much more flavors. All groups were abundant in unsaturated FAs, including eicosapentaenoic acid(EPA) and docosahexaenoic acid(DHA). In terms of content and variety, the FAs in Group B were more beneficial to human health than those in the other groups. The content of each AA in Group B was basically higher than those in the other groups and was significantly higher than that in Group D(P < 0.05). Comparing the VFSs, FAs, and AAs in samples fed with four kinds of diets, the results indicate that using crab to feed O. vulgaris can achieve better effects on volatile flavor and nutritional ingredients. | LUO Qihao WANG Weijun LI Zan ZHU Xinghua WANG Xue ZHANG Tonghua XU He YANG Jianmin | 2021 | Journal of Ocean University of China2021，20，2： | 1 |
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