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| 1 | GSDMB promotes non-canonical pyroptosis by enhancing caspase-4 activity显示文摘Gasdermin B (GSDMB) has been reported to be associated with immune diseases in humans, but the detailed molecular mechanisms remain unsolved. The N-terminus of GSDMB by itself, unlike other gasdermin family proteins, does not induce cell death. Here, we show that GSDMB is highly expressed in the leukocytes of septic shock patients, which is associated with increased release of the gasdermin D (GSDMD) N-terminus. GSDMB expression and the accumulation of the N-terminal fragment of GSDMD are induced by the activation of the non-canonical pyroptosis pathway in a human monocyte cell line. The downregulation of GSDMB alleviates the cleavage of GSDMD and cell death. Consistently, the overexpression of GSDMB promotes GSDMD cleavage, accompanied by increased LDH release. We further found that GSDMB promotes caspase-4 activity, which is required for the cleavage of GSDMD in non-canonical pyroptosis, by directly binding to the CARD domain of caspase-4. Our study reveals a GSDMB-mediated novel regulatory mechanism for non-canonical pyroptosis and suggests a potential new strategy for the treatment of inflammatory diseases. | Qin Chen Peiliang Shi Yufang Wang Dayuan Zou Xiuwen Wu Dingyu Wang Qiongyuan Hu Yujie Zou Zan Huang Jianan Ren Zhaoyu Lin Xiang Gao | 2019 | Journal of Molecular Cell Biology2019，11，6： | 22 |
| 2 | Mitochondrial ROS promote macrophage pyroptosis by inducing GSDMD oxidation显示文摘Disrupted mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) generation are often associated with macrophage pyroptosis. It remains unclear how these forms of mitochondrial dysfunction relate to inflammasome activation and gasdermin-D (Gsdmd) cleavage, two central steps of the pyroptotic process. Here, we also found MMP collapse and ROS generation induced by Nlrp3 inflammasome activation as previous studies reported. The elimination of ROS alleviated the cleavage of Gsdmd, suggesting that Gsdmd cleavage occurs downstream of ROS release. Consistent with this result, hydrogen peroxide treatment augmented the cleavage of Gsdmd by caspase-1. Indeed, four amino acid residues of Gsdmd were oxidized under oxidative stress in macrophages. The efficiency of Gsdmd cleavage by inflammatory caspase-1 was dramatically reduced when oxidative modification was blocked by mutation of these amino acid residues. These results demonstrate that Gsdmd oxidation serves as a de novo mechanism by which mitochondrial ROS promote Nlrp3 inflammasome-dependent pyroptotic cell death. | Yufang Wang Peiliang Shi Qin Chen Zan Huang Dayuan Zou Jingzi Zhang Xiang Gao Zhaoyu Lin | 2019 | Journal of Molecular Cell Biology2019，11，12： | 23 |
| 3 | A Fuzzy PID Algorithm for a Novel Miniature Spherical Robots with Three-dimensional Underwater Motion Control显示文摘We proposed and developed a small bionic amphibious spherical robot system for tasks such as coastal environment monitoring and offshore autonomous search and rescue.Our third-generation bionic small amphibious spherical robots have many disadvantages,such as the lack of maneuverability and a small operating range.It is difficult to accomplish underwater autonomous motion control with these robots.Therefore,we proposed a fourth-generation amphibious spherical robot.However,the amphibious spherical robot developed in this project has a small and compact design,with limited sensors and external sensing options.This means that the robot has weak external information collection capabilities.We need to make the real time operation of the robot's underwater motion control system more reliable.In this paper,we mainly used a fuzzy Proportional-Integral-Derivative(PID)control algorithm to design an underwater motion control system for a novel robot.Moreover,we compared PID with fuzzy PID control methods by carrying out experiments on heading and turning bow motions to verify that the fuzzy PID is more robust and exhibits good dynamic performance.We also carried out experiments on the three-dimensional(3D)motion control to validate the design of the underwater motion control system. | Liwei Shi Yao Hu Shuxiang Su Shuxiang Guo Huiming Xing Xihuan Hou Yu Liu Zhan Chen Zan Li Debin Xia | 2020 | Journal of Bionic Engineering2020，17，5： | 4 |
| 4 | An injectable and self-healing hydrogel with controlled release of curcumin to repair spinal cord injury显示文摘The harsh local micro-environment following spinal cord injury(SCI)remains a great challenge for neural regeneration.Local reconstitution of a favorable micro-environment by biocompatible scaffolds with desirable functions has thus been an area of concern.Herein,a hybrid hydrogel was developed using Fmoc-grafted chitosan(FC)and Fmoc peptide(FI).Dynamic reversibleπ-πstacking interactions of the fluorenyl rings enabled the FC/FI hybrid hydrogel to exhibit excellent injectable and self-healing properties,as characterized by visual appearances and rheological tests.Furthermore,the FC/FI hybrid hydrogel showed a slow and persistent release of curcumin(Cur),which was named as FC/FI-Cur hydrogel.In vitro studies confirmed that with the support of FC/FI-Cur hydrogel,neurite outgrowth was promoted,and Schwann cell(SC)migration away from dorsal root ganglia(DRG)spheres with enhanced myelination was substantiated.The FC/FI-Cur hydrogel well reassembled extracellular matrix at the lesion site of rat spinal cord and exerted outstanding effects in modulating local inflammatory reaction by regulating the phenotypes of infiltrated inflammatory cells.In addition,endogenous SCs were recruited in the FC/FI-Cur graft and participated in the remyelination process of the regenerated nerves.These outcomes favored functional recovery,as evidenced by improved hind limbs movement and enhanced electrophysiological properties.Thus,our study not only advanced the development of multifunctional hydrogels but also provided insights into comprehensive approaches for SCI repair. | Jinghua Luo Xueshuang Shi Liming Li Zan Tan Feng Feng Jun Li Mao Pang Xiaoying Wang Liumin He | 2021 | Bioactive Materials2021，6，12： | 4 |
| 5 | Oil production rate predictions for steam assisted gravity drainage based on high-pressure experiments显示文摘Dual-well steam assisted gravity drainage(SAGD) has significant potential for extra-heavy oil recovery.China is conducting two dual-well SAGD pilot projects in the Fengcheng extra-heavy oil reservoir.Quick,direct predictions of the oil production rate by algebraic models rather than complex numerical models are of great importance for designing and adjusting the SAGD operations.A low-pressure scaled physical simulation was previously used to develop two separate theoretical models corresponding to the two different growth stages observed in the SAGD steam chambers,which are the steam chamber rising stage and the steam chamber spreading stage.A high-pressure scaled model experiment is presented here for one dual-well SAGD pattern to further improve the prediction models to reasonably predict oil production rates for full production.Parameters that significantly affect the oil recovery during SAGD were scaled for the model size based on the reservoir characteristics of the Fengcheng reservoir in China.Experimental results show the relationship between the evolution of the steam chamber and the oil production rate during the entire production stage.High-pressure scaled model test was used to improve the gravity drainage models by modifying empirical factors for the rising model and the depletion model.A new division of the SAGD production regime was developed based on the relationship between the oil production rate and the evolution of steam chamber.A method was developed to couple the rising and depletion models to predict oil production rates during the SAGD production,especially during the transition period.The method was validated with experiment data and field data from the literature.The model was then used to predict the oil production rate in the Fengcheng reservoir in China and the Athabasca reservoir in Canada. | GUO Jia ZAN Cheng MA DeSheng SHI Lin | 2013 | Science China(Technological Sciences)2013，56，2： | 2 |
| 6 | CD4+T cells memorize obesity and promote weight regain显示文摘Body weight regain often causes failure of obesity therapies while the underlying mechanism remains largely unknown.In this study,we report that immune cells,especially CD4+T cells,mediate the‘memory’of previous obese status.In a weight gain-loss-regain model,we found that C57BL/6J mice with an obesity history showed a much faster rate of body weight regain.This obesity memory could last for at least 2 months after previously obese mice were kept at the same body weight as non-obese mice.Surprisingly,such obesity memory was abrogated by dexamethasone treatment,whereas immunodeficient Rag1−/−and H2A−/−mice failed to establish such memory.Rag1−/−mice repossessed the obesity memory when immune cells or CD4+T cells isolated from previously obese mice were transferred.Furthermore,depletion of CD4+T cells led to obesity memory ablation.Taken together,we conclude that CD4+T cells mediate obesity memory and promote weight regain. | Jianghuan Zou Beibei Lai Mingzhu Zheng Qin Chen Shujun Jiang Anying Song Zan Huang Peiliang Shi Xin Tu Di Wang Linrong Lu Zhaoyu Lin Xiang Gao | 2018 | Cellular & Molecular Immunology2018，15，6： | 2 |
| 7 | Compound Gaoziban tablet(复方高滋斑片) alleviates depression via toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-kappa B pathway显示文摘OBJECTIVE: To evaluate the effect of compound Gaoziban tablet(复方高滋斑片, CGZBT) on depression, and to investigate the underlying mechanism. METHODS: The components of CGZBT were analysed by high-performance liquid chromatography. Then, we assessed the effects of varying doses of CGZBT on an established chronic unpredictable mild stress(CUMS) model in rats. Whether animals were depressed was evaluated by sucrose preference test, open field test and forced swimming test. Neurotransmitters of hippocampus were detected by liquid chromatography-mass spectrometry. Serum levels of tumor necrosis factor-alpha(TNF-α), interleukin(IL)-1β, IL-6, IL-4, and IL-10 were measured by enzyme-linked immunosorbent assay. Expressions of toll-like receptor 4(TLR4), myeloid differentiation factor 88(My D88), phospho-nuclear factorkappa B(p-NF-κB), cyclooxygenase-2(COX-2), ionized calcium binding adapter molecule-1(IBA-1) were assessed by immunohistochemical staining and western blotting. RESULTS: Eight compounds were identified from CGZBT, moreover, our results showed that CGZBT effectively reversed the CUMS-induced decrease in sucrose preference, shortened the movement distance and prolonged immobility time. CGZBT significantly increased levels of 5-hydroxytryptamine, dopamine, norepinephrine, 5-hydroxyindoleacetic acid levels, and reduced the expression of TNF-α, IL-1β, IL-6, yet increased IL-4 and IL-10. Furthermore, the expressions of TLR4, My D88, COX-2, p-NF-κB and IBA-1 in hippocampus were effectively reversed after treatment with CGZBT. CONCLUSIONS: These results indicated that CGZBT could, at least in part, alleviate depression induced by CUMS via the TLR4/My D88/NF-κB pathway, suggesting its potential as an antidepressant drug. | ZOU Xinshuang SHI Lei YIN Hailong LI Haiping WANG Mengheng SONG Wanci LUO Laichun WU Hezhen YANG Yanfang ZAN Junfeng LIU Yanwen DAN Hanxiong YIN Qiang YOU Pengtao | 2022 | Journal of Traditional Chinese Medicine2022，42，6： | 2 |
| 8 | Research methods and performance analysis for the moderately high temperature refrigerant显示文摘In China, directly dropping high temperature refrigerant into conventional com- pressor is considered as a dominant technical route for development of moderately high temperature heat pump. Based on this route, selection criteria for high tem- perature refrigerant were presented with consideration of several influencing fac- tors. Moreover, a set of research methods were built including theoretical calcula- tions and experimental tests. Four high temperature refrigerants from HTR01 to HTR04 were compared and analyzed. In results, firstly, HTR03 and HTR04 belonging to non-ozone depleting refrigerants could bring outstanding environmental benefits; secondly, the condenser outlet water efficiently generated by heat pump using high temperature refrigerants could cover 60℃―90℃; finally, the feasibility of the tech- nical route and the research methods were proved. | SHI Lin ZAN Cheng | 2008 | Science China(Technological Sciences)2008，51，8： | 2 |
| 9 | Simultaneous yuantilication of multiple active components from Xiexin decoction in rat plasma by LC-ESI-MS/MS:application in pharmacokinetics显示文摘 | Zan B Shi R Wang TM | 2011 | Biomed Chromatogr2011，25，7： | 1 |
| 10 | Performance limitations of a Y-branch directional coupler-based polymeric high-speed electro-optical modulator 显示文摘 | ZHOU Qing-Jun YANG Jian-Yi SHI Zan | 2004 | Opt Eng2004，43，4： | 1 |
| 11 | Numerical simulation of material flow behavior of friction stir welding influenced by rotational tool geometry显示文摘 | S.D. Ji Q.Y. Shi L.G. Zhang A.L. Zou S.S. Gao L.V. Zan | 2012 | Computational Materials Science2012，，： | 1 |
| 12 | Highly dispersed Mn_2O_3 microspheres:Facile solvothermal synthesis and their application as Li-ion battery anodes显示文摘Nanostructured transition metal oxides are promising alternative anodes for lithium ion batteries.Li-ion storage performance is expected to improve if high packing density energy particles are available.Herein,Mn_2O_3 microspheres with a ca.18 μm diameter and a tapped density of 1.33 g/cm^3 were synthesized by a facile solvothermal-thermal coversion route.Spherical MnCO_3 precursors were obtained through solvothermal treatment and they decomposed and converted into Mn_2O_3 microspheres at an annealing temperature of 700 C.The Mn_2O_3 microspheres consisted of Mn_2O_3 nanoparticles with an average 40 nm diameter.These porous Mn_2O_3 microspheres allow good electrolyte penetration and provide an ion buffer reservoir to ensure a constant electrolyte supply.The Mn_2O_3 microspheres have reversible capacities of590 and 320 mAh/g at 50 and 400 mA/g,respectively.We thus report an efficient route for the fabrication of energy particles for advanced energy storage. | Jianfei Yu Lin Zhu Cheng Fan Cheng Zan Ling Hu Shuhui Yang Qiang Zhang Wancheng Zhu Lin Shi Fei Wei | 2015 | Particuology2015，13，5： | 1 |
| 13 | Effect of adding method of Cr on microstructure and properties of WC–9Ni–2Cr cemented carbides显示文摘 | Kai-hua Shi Ke-chao Zhou Zhi-you Li Xiu-qi Zan Shang-zhi Xu Zhao-yu Min | 2012 | International Journal of Refractory Metals and Hard Materials2012，，： | 1 |
| 14 | Large-Scale Application of Municipal Reclaimed Water Source Heat Pump to a DHC System in the Beijing Olympic Village显示文摘 | Shi L Zan C | 2008 | 9th IEA Heat Pump Conference2008，，： | 1 |
| 15 | Evolution of composite fouling on a vertical stainless steel surface caused by treated sewage显示文摘 | Cheng Zan Lin Shi Wenyan Yang Xiujuan Ma | 2010 | Research article Front Energy Power Eng China 20102010，4，2： | 1 |
| 16 | A new polystyrene-TiO2 nano composite film and its photocatalytic degradation显示文摘 | LING Zan LI Hongtian ZHONG Shi liu | | 0，，： | 1 |
| 17 | Secrecy Transmission of NOMA-OTFS Based Multicast-Unicast Streaming显示文摘This paper investigates the security performance of a cooperative multicast-unicast system,where the users present the feature of high mobility.Specifically,we develop the non-orthogonal multiple access(NOMA)based orthogonal time frequency space(OTFS)transmission scheme,namely NOMAOTFS,in order to combat Doppler effect as well as to improve the spectral efficiency.Further,we propose a power allocation method addressing the trade-off between the reliability of multicast streaming and the confidentiality of unicast streaming.Based on that,we utilize the relay selection strategy,to improve the security of unicast streaming.In the context of multicastunicast streaming,our simulation findings validate the effectiveness of the NOMA-OTFS based cooperative transmission,which can significantly outperform the existing NOMA-OFDM in terms of both reliability and security. | Junfan Hu Jia Shi Xianyu Wang Xiaoju Lu Zan Li Zhuangzhuang Tie Quanxue Gao | 2023 | China Communications2023，20，1： | 1 |
| 18 | Quinolone alkaloids with antibacterial and cytotoxic activities from the fruits of Evodia rutaecarpa显示文摘 | Wang X X Zan K Shi S P | 2013 | Fitoterapia2013，89，7： | 1 |
| 19 | Ex- perimental research of critical condensate saturation and flow characteristics of gas condensate reservoir显示文摘 | Li Qian Li Xiangfang Zan Ke Shi Juntai Song Zhaojie | 2013 | Petroleum Sci- ence and Technology2013，31，13： | 1 |
| 20 | Evolution of Composite Fouling on A Vertical Stainless Steel Surface Caused by Treated Sewage 显示文摘 | ZAN Cheng SHI Lin YANG Wenyan | 2010 | Front Energy2010，4，2： | 1 |
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