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| 1 | Gastric cancer: Epidemiology, risk factors and prevention strategies显示文摘Gastric cancer(GC) is a global health problem, with more than 1 million people newly diagnosed with GC worldwide each year. GC is more prevalent in less developed countries than in more developed countries. About half of all GC cases worldwide occur in East Asia, notably China. Globally, overall incidence rates of GC are declining, which is potentially attributed to a decrease in Helicobacter pylori(H. pylori) infection and the use of refrigeration to preserve foods rather than salt. GC is a multifactorial disease, and its occurrence and development were impacted by environmental and genetic factors. H. pylori infection is the primary risk factor for GC, especially for non-cardia. The prognosis of GC is poor due to stages at the first diagnosis. The 5-year survival rate is less than 10% when patients are diagnosed at an advanced stage, but the rate is as high as 85% if patients are detected at an earlier stage. Endoscopic screening can potentially prevent GC by early diagnosis and early treatment and has been widely adopted in screening programs in East Asian countries, such as Japan and Korea. This review summarizes updated epidemiological aspects, risk factors, and prevention strategies of GC in recent years to help researchers determine the most effective intervention strategies for reducing risk of GC. | Lei Yang Xiangji Ying Shuo Liu Guoqing Lyu Zekuan Xu Xi Zhang Huichao Li Qingyu Li Ning Wang Jiafu Ji | 2020 | Chinese Journal of Cancer Research2020，32，6： | 52 |
| 2 | Aspirin suppresses growth of human gastric carcinoma cell by inhibiting survivin expression显示文摘Regular use of aspirin (ASA) could reduce the risk of gastric cancer although the precise mechanism remains unclear.Down-regulation of survivin may be one of the cyclooxygenase-independent mechanisms whereby ASA induces apoptosis of gastric cancer cell.In this study,we investigated the effect of ASA on the growth,apoptosis and survivin expression of gastric cancer cell line SGC7901.The survival of cells treated with 3.0 and 10.0 mmol/L ASA for 24 h was decreased by 44.6% and 88.5%,respectively.ASA at 3.0 mmol/L inhibited the viability of SGC7901 cells in a time-dependent manner.Apoptosis analysis showed similar results with MTT assay.ASA at 3.0 and 10.0 mmol/L decreased the mRNA transcript levels of survivin and reduced survivin protein levels in SGC7901 cells also in a time-dependent manner.Our findings indicated that ASA inhibited the proliferation of SGC7901 by suppressing survivin at both the transcriptional and translational level. | Li Yang Huaijun Zhu Dongxiao Liu Song Liang Hao Xu Jie Chen Xuerong Wang Zekuan Xu | 2011 | The Journal of Biomedical Research2011，25，4： | 9 |
| 3 | A machine learning-based algorithm used to estimate the physiological elongation of ocular axial length in myopic children显示文摘Background:Axial myopia is the most common type of myopia.However,due to the high incidence of myopia in Chinese children,few studies estimating the physiological elongation of the ocular axial length(AL),which does not cause myopia progression and differs from the non-physiological elongation of AL,have been conducted.The purpose of our study was to construct a machine learning(ML)-based model for estimating the physiological elongation of AL in a sample of Chinese school-aged myopic children.Methods:In total,1011 myopic children aged 6 to 18 years participated in this study.Cross-sectional datasets were used to optimize the ML algorithms.The input variables included age,sex,central corneal thickness(CCT),spherical equivalent refractive error(SER),mean K reading(K-mean),and white-to-white corneal diameter(WTW).The output variable was AL.A 5-fold cross-validation scheme was used to randomly divide all data into 5 groups,including 4 groups used as training data and one group used as validation data.Six types of ML algorithms were implemented in our models.The best-performing algorithm was applied to predict AL,and estimates of the physiological elongation of AL were obtained as the partial derivatives of AL_(predicted)-age curves based on an unchanged SER value with increasing age.Results:Among the six algorithms,the robust linear regression model was the best model for predicting AL,with a R^(2) value of 0.87 and relatively minimal averaged errors between the predicted AL and true AL.Based on the partial derivatives of the AL_(predicted)-age curves,the estimated physiological AL elongation varied from 0.010 to 0.116 mm/year in male subjects and 0.003 to 0.110 mm/year in female subjects and was influenced by age,SER and K-mean.According to the model,the physiological elongation of AL linearly decreased with increasing age and was negatively correlated with the SER and the K-mean.Conclusions:The physiological elongation of the AL is rarely recorded in clinical data in China.In cases of unavailable clinical dat,an ML algorithm could provide practitioners a reasonable model that can be used to estimate the physiological elongation of AL,which is espedally useful when monitoring myopia progression in orthokeratology lens wearers. | Tao Tang Zekuan Yu Qiong Xu Zisu Peng Yuzhuo Fan Kai Wang Qiushi Ren Jia Qu Mingwei Zhao | 2020 | Eye and Vision2020，7，1： | 4 |
| 4 | Laparoscopic versus open total gastrectomy with D2 dissection for gastric cancer: a meta-analysis显示文摘 | Weizhi Wang Zheng Li Jie Tang Meilin Wang Baolin Wang Zekuan Xu | 2013 | Journal of Cancer Research and Clinical Oncology2013，，10： | 4 |
| 5 | Upregulation of the eIF4E signaling pathway contributes to the progressionof gastric cancer, and targeting eIF4E by perifosine inhibits cell growth显示文摘 | Song Liang Renhua Guo Zhihong Zhang Dongxiao Liu Hao Xu Zekuan Xu Xuerong Wang Li Yang | 2013 | Oncology Reports2013，，6： | 2 |
| 6 | Cytosolic TGM2 promotes malignant progression in gastric cancer by suppressing the TRIM21-mediated ubiquitination/degradation of STAT1 in a GTP binding-dependent modality显示文摘Background:Previous studies have revealed the critical role of transglutaminase 2(TGM2)as a potential therapeutic target in cancers,but the oncogenic roles and underlying mechanisms of TGM2 in gastric cancer(GC)are not fully understood.In this study,we examined the role and potential mechanism of TGM2 in GC.Methods:Western blotting,immunohistochemistry,CCK8,colony formation and transwell assays were used to measure TGM2 expression in the GC cells and tissues and to examine the in vitro role of TGM2 in GC.Xenograft and in vivo metastasis experiments were performed to examine the in vivo role of TGM2 in GC.Gene set enrichment analysis,quantitative PCR and western blotting were conducted to screen for potential TGM2 targets involved in GC.Gain/loss-offunction and rescue experiments were conducted to detect the biological roles of STAT1 in GC cells in the context of TGM2.Co-immunoprecipitation,mass spectrometry,quantitative PCR and western blotting were conducted to identify STAT1-interacting proteins and elucidate their regulatory mechanisms.Mutations in TGM2 and two molecules(ZM39923 and A23187)were used to identify the enzymatic activity of TGM2 involved in the malignant progression of GC and elucidate the underlying mechanism.Results:In this study,we demonstrated elevated TGM2 expression in the GC tissues,which closely related to pathological grade,and predicted poor survival in patients with GC.TGM2 overexpression or knockdown promoted(and inhibited)cell proliferation,migration,and invasion,which were reversed by STAT1 knockdown or overexpression.Further studies showed that TGM2 promoted GC progression by inhibiting STAT1 ubiquitination/degradation.Then,tripartite motif-containing protein 21(TRIM21)was identified as a ubiquitin E3 ligase of STAT1 in GC.TGM2 maintained STAT1 stability by facilitating the dissociation of TRIM21 and STAT1 with GTP-binding enzymatic activity.A23187 abolished the role of TGM2 in STAT1 and reversed the pro-tumor role of TGM2 in vitro and in vivo.Conclusions:This study revealed a critical role and regulatory mechanism of TGM2 on STAT1 in GC and highlighted the potential of TGM2 as a therapeutic target,which elucidates the development of medicine or strategies by regulating the GTP-binding activity of TGM2 in GC. | Lu Zhang Qingya Li Jing Yang Penghui Xu Zhe Xuan Jianghao Xu Zekuan Xu | 2023 | Cancer Communications2023，43，1： | 1 |
| 7 | CEACAM6 induces epithelial-mesenchymal transition and mediates invasionand metastasis in pancreatic cancer显示文摘 | Jianmin Chen Qiang Li Yong An Nan Lv Xiaofeng Xue Jishu Wei Kuirong Jiang Junli Wu Wentao Gao Zhuyin Qian Cuncai Dai Zekuan Xu Yi Miao | 2013 | International Journal of Oncology2013，，3： | 1 |
| 8 | Laparoscopic versus open total gastrectomy with D2 dissection for gastric cancer: a meta-analysis显示文摘 | Weizhi Wang Zheng Li Jie Tang Meilin Wang Baolin Wang Zekuan Xu | 2013 | Journal of Cancer Research and Clinical Oncology2013，，10： | 1 |
| 9 | miR-874 Inhibits cell proliferation, migration and invasion through targeting aquaporin-3 in gastric cancer显示文摘 | Baofei Jiang Zengliang Li Wenjie Zhang Haixiao Wang Xiaofei Zhi Jin Feng Zheng Chen Yi Zhu Li Yang Hao Xu Zekuan Xu | 2014 | Journal of Gastroenterology2014，，6： | 1 |
| 10 | Studies of CTLA4Ig in acute rejection of pancreas transplantation in rats显示文摘Objective: To investigate the protective effect of CTLA4Ig in rejection of pancreaticoduodenal transplantation model of rat. Methods: Pancreaticoduodenal transplantion models were established from the donor F344 rats to the Lewis recipients. The models were divided into 2 groups: Group A and B with 12 rats in each group.2 days after transplantation, reciepients in group A were treated with i.p. injection of sailine, and those in group B CTLA4I were injected(200g). On day 1,4,7,10 after transplantation, the grafts were harvested for histopathological examination. On day 4 after transplantation, the CD4+CD25+ T cells in the grafts were detected by Flow Cytometry. Results: Compared with group A: the degree of the rejection of grafts in group B was lower. The number of CD4+CD25+ T cells of graft was (7.91 ± 1.26)% in group A and (13.81 ± 1.71)% in group B, which had significant difference(P < 0.01). Conclusion: CTLA4Ig could inhibit T cell costimulatory pathway, prevent acute rejection, which might be mediated by increasing the number of CD4+CD25+ regulatory T cells. | Junbo Yu Zekuan Xu Shuguang Han Yi Miao | 2006 | Journal of Nanjing Medical University2006，20，5： | 0 |
| 11 | N6-methyladenosine modification of CENPF mRNA facilitates gastric cancer metastasis via regulating FAK nuclear export显示文摘Background:N6-methyladenosine(m^(6)A)modification is the most common modification that occurs in eukaryotes.Although substantial effort has been made in the prevention and treatment of gastric cancer(GC)in recent years,the prognosis of GC patients remains unsatisfactory.The regulatory mechanism between m^(6)A modification and GC development needs to be elucidated.In this study,we examined m^(6)A modification and the downstream mechanism in GC.Methods:Dot blotting assays,The Cancer Genome Atlas analysis,and quantitative real‑time PCR(qRT-PCR)were used to measure the m^(6)A levels in GC tissues.Methylated RNA-immunoprecipitation sequencing and RNA sequencingwere performed to identify the targets ofm^(6)Amodification.Western blotting,Transwell,wound healing,and angiogenesis assays were conducted to examine the role of centromere protein F(CENPF)in GC in vitro.Xenograft,immunohistochemistry,and in vivo metastasis experiments were conducted to examine the role of CENPF in GC in vivo.Methylated RNA-immunoprecipitation-qPCR,RNA immunoprecipitation-qPCR and RNA pulldown assays were used to verify the m^(6)A modification sites of CENPF.Gain/loss-of-function and rescue experiments were conducted to determine the relationship between CENPF and the mitogen-activated protein kinase(MAPK)signaling pathway in GC cells.Coimmunoprecipitation,mass spectrometry,qRT-PCR,and immunofluorescence assays were performed to explore the proteins that interact with CENPF and elucidate the regulatory mechanisms between them.Results:CENPF was upregulated in GC and facilitated the metastasis of GC both in vitro and in vivo.Mechanistically,increasedm^(6)A modification of CENPF was mediated by methyltransferase 3,and this modified molecule could be recognized by heterogeneous nuclear ribonucleoprotein A2/B1(HNRNPA2B1),thereby promoting its mRNA stability.In addition,the metastatic phenotype of CENPF was dependent on the MAPK signaling pathway.Furthermore,CENPF could bind to FAK and promote its localization in the cytoplasm.Moreover,we discovered that high expression of CENPF was related to lymphatic invasion and overall survival in GC patients.Conclusions:Our findings revealed that increased m^(6)A modification of CENPF facilitates the metastasis and angiogenesis of GC through the CENPF/FAK/MAPK and epithelial-mesenchymal transition axis.CENPF expression was correlated with the clinical features of GC patients;therefore,CENPF may serve as a prognostic marker of GC. | Penghui Xu Jing Yang Zetian Chen Xing Zhang Yiwen Xia Sen Wang Weizhi Wang Zekuan Xu | 2023 | Cancer Communications2023，43，6： | 0 |
| 12 | Growth hormone receptor expression in human primary gastric adenocarcinoma显示文摘The aim of this study was to determine the expression of growth hormone receptor(GHR) in patients with pri-mary gastric adenocarcinoma.We investigated 48 specimens of primary gastric adenocarcinoma and their cor-responding normal gastric mucosa.Immunohistochemistry and reverse transcription-polymerase chain reaction(RT-PCR) were used to detect the expression of GHR.Immunohistochemical analyses revealed that GHR was expressed in human primary gastric adenocarcinoma(36/48,75.0%) and appeared to be upregulated,compared to the normal mucosa(28/48,58.3%,P < 0.001).A significant correlation was found between GHR expression and tumor stage(P < 0.001) and tumor differentiation(P < 0.001).The average positive rate of ki-67 in GHR-positive tumors was 16.06%,while the positive rate in GHR-negative tumors was 6.17%(P < 0.01).The average apoptosis index(AI) of GHR-positive tumors was 3.36%,which was significantly lower than that(7.33%) of GHR-negative tumors.In addition,27 of 48 cases of tumors had GHR mRNA expression,while only 17 of all 48 cases of normal mucosa did so.Our results indicate that the frequency of GHR was significantly higher in primary gastric adeno-carcinoma than that in normal gastric mucosa.GHR expression was significantly correlated with tumor differen-tiation and tumor grade.This finding supported a possible role of growth hormone in primary gastric adenocarci-noma pathophysiology. | Xiaodong Yang Ping Huanga Feng Wang Zekuan Xu Xiaonin Wang | 2012 | The Journal of Biomedical Research2012，26，5： | 0 |
| 13 | The Investigation and Analysis of the Situation of the Outflowing Peasants in 24 Villages | Wu Huailian Xia Zekuan | 1990 | China City Planning Review1990，7，4： | 0 |
| 14 | Establishment of simultaneous pancreas and kidney transplantation (SPK) model with cuff technique and portal venous drainage in rats显示文摘Objective: To establish a simultaneous pancreas and kidney transplantation (SPK) model in the rat. Methods: SD rats served as donors and recipients. The donor portal vein and the recipient superior mesenteric vein were anastomosed and the donor renal veins and recipient renal veins were anastomosed by cuff method. Arterial reconstruction was carried out by end to side anastomosis of the donor abdominal aorta to the recipient abdominal aorta. Enteric drainage was performed by side to side anastomosis between donors’ duodenum and recipients’ jejunum. The donor ureter -bladder valve was anastomosed to the bladder of recipients. Results: Out of 30 cases of SPK transplantation, 24 had normal serum glucose and serum creatinine after operation. The successful rate was 80 %. Conclusion: This model of SPK in rats is stable and reliable, which could be applied for further scientific research. | Shuguang Han Zekuan Xu Xuan Zhang Yi Miao | 2007 | Journal of Nanjing Medical University2007，21，1： | 0 |
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