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| 1 | DSDecode: A Web-Based Tool for Decoding of Sequencing Chromatograms for Genotyping of Targeted Mutations显示文摘Dear Editor, The transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 genome editing systems have greatly improved the efficiency for generating targeted mutations in various organisms including plants (Li et al.,2012;Cong et al.,2013;Li et al.,2013;Feng et al.,2014;Ma et al.,2015b;Zhang et al.,2014,2015). | Weizhi Liu Xianrong Xie Xingliang Ma Jun Li Jiehu Chen Yao-Guang Liu | 2015 | Molecular Plant2015，8，9： | 62 |
| 2 | One-step generation of p53 gene biallelic mutant Cynomolgus monkey via the CRISPR/Cas system显示文摘 | Haifeng Wan Chunjing Feng Fei Teng Shihua Yang Baoyang Hu Yuyu Niu Andy Peng Xiang Weizhen Fang Weizhi Ji Wei Li Xiaoyang Zhao Qi Zhou | 2015 | Cell Research2015，25，2： | 39 |
| 3 | A Novel 3D Non-Stationary UAV-MIMO Channel Model and Its Statistical Properties显示文摘The wireless communication systems based on Unmanned Aerial Vehicles(UAVs) have found a wide range of applications recently. In this paper, we propose a new three-dimensional(3 D) non-stationary multiple-input multiple-output(MIMO) channel model for the communication links between the UAV and mobile terminal(MT). The new model originates the traditional geometry-based stochastic models(GBSMs) but considers the non-stationary propagation environment due to the rapid movements of the UAV, MT, and clusters. Meanwhile, the upgrade time evolving algorithms of time-variant channel parameters, i.e., the path number based on birth-death processes of clusters, path delays, path powers, and angles of arrival and departure, are developed and optimized. In addition, the statistical properties of proposed GBSM including autocorrelation function(ACF), cross-correlation function(CCF), and Doppler power spectrum density(DPSD) are investigated and analyzed. Simulation results demonstrate that our proposed model provides a good agreement on the statistical properties with the corresponding derived theoretical ones, which indicates its usefulness for the performance evaluation and validation of the UAV based communication systems. | Qiuming Zhu Kaili Jiang Xiaomin Chen Weizhi Zhong Ying Yang | 2018 | China Communications2018，15，12： | 18 |
| 4 | Generation of a precise Oct4-hrGFP knockin cynomolgus monkey model via CRISPR/Cas9-assisted homologous recombination显示文摘 | Yiqiang Cui Yuyu Niu Jiankui Zhou Yongchang Chen Yiwei Cheng Shangang Li Zongyong Ai Chu Chu Hong Wang Bo Zheng Xuejin Chen Jiahao Sha Xuejiang Guo Xingxu Huang Weizhi Ji | 2018 | Cell Research2018，28，3： | 13 |
| 5 | Polybrominated diphenyl ethers in atmosphere and soil of a production area in China:Levels and partitioning显示文摘Polybrominated diphenyl ethers (PBDEs) were measured in atmosphere and soil samples taken in winter and summer at a PBDE production area of Laizhou Bay in China.The concentrations of 11 PBDE were 0.017-1.17 ng/m 3 in gaseous phase,0.5-161.1 ng/m 3 in particulate phase,and 73-2629 ng/g dry weight in soil samples.The PBDE congener pattern in the gaseous phase differed from that in the particulate phase,and the PBDE congener pattern in the particulate phase was similar with that in soil.This demonstrated that there was little difference with atmospheric particle-soil transfer efficiency among PBDE congeners.In addition,there were seasonal variations in percentages on particle for lower brominated congeners.The BDE-28 was mostly in the gaseous phase in summer (88.3%),whereas the average proportion of BDE-28 in gaseous phase in winter was 38.9%.Higher brominated congeners (i.e.,BDE-206,BDE207,BDE-208,and BDE-209) were bound to the atmospheric particulate phase,and their potentials for long-range migration were mainly affected by the environmental behavior of atmospheric particles.Results indicated that PBDE congeners in summer were closer to gas-particle partition equilibrium than in winter.Temperature should be considered the main factor causing nonequilibrium in winter. | Jun Jin Ying Wang Weizhi Liu Congqiao Yang Jicheng Hu Jian Cui | 2011 | Journal of Environmental Sciences2011，23，3： | 12 |
| 6 | Transgenic rhesus monkeys carrying the human MCPH1 gene copies show human-like neoteny of brain development显示文摘Brain size and cognitive skills are the most dramatically changed traits in humans during evolution and yet the genetic mechanisms underlying these human-specific changes remain elusive.Here,we successfully generated 11 transgenic rhesus monkeys(8 first-generation and 3 second-generation)carrying human copies of MCPH1,an important gene for brain development and brain evolution.Brain-image and tissue-section analyses indicated an altered pattern of neural-cell differentiation,resulting in a delayed neuronal maturation and neural-fiber myelination of the transgenic monkeys,similar to the known evolutionary change of developmental delay(neoteny)in humans.Further brain-transcriptome and tissue-section analyses of major developmental stages showed a marked human-like expression delay of neuron differentiation and synaptic-signaling genes,providing a molecular explanation for the observed brain-developmental delay of the transgenic monkeys.More importantly,the transgenic monkeys exhibited better short-term memory and shorter reaction time compared with the wild-type controls in the delayed-matching-to-sample task.The presented data represent the first attempt to experimentally interrogate the genetic basis of human brain origin using a transgenic monkey model and it values the use of non-human primates in understanding unique human traits. | Lei Shi Xin Luo Jin Jiang Yongchang chen Cirong Liu Ting Hu Min Li Qiang Lin Yanjiao Li Jun Huang Hong Wang Yuyu Niu Yundi Shi Martin Styner Jianhong Wang Yi Lu Xuejin Sun Hualin Yu Weizhi Ji Bing Su | 2019 | National Science Review2019，6，3： | 12 |
| 7 | Generation of a Hutchinson-Gilford progeria syndrome monkey model by base editing显示文摘Many human genetic diseases,including Hutchinson-Gilford progeria syndrome(HGPS),are caused by single point mutations.HGPS is a rare disorder that causes premature aging and is usually caused by a de novo point mutation in the LMNA gene.Base editors(BEs)composed of a cytidine deaminase fused to CRISPR/Cas9 nickase are highly efficient at inducing C to T base conversions in a programmable manner and can be used to generate animal disease models with single amino-acid substitutions.Here,we generated the first HGPS monkey model by delivering a BE mRNA and guide RNA(gRNA)targeting the LMNA gene via microinjection into monkey zygotes.Five out of six newborn monkeys carried the mutation specifically at the target site.HGPS monkeys expressed the toxic form of lamin A,progerin,and recapitulated the typical HGPS phenotypes including growth retardation,bone alterations,and vascular abnormalities.Thus,this monkey model genetically and clinically mimics HGPS in humans,demonstrating that the BE system can efficiently and accurately generate patient-specific disease models in non-human primates. | Fang Wang Weiqi Zhang Qiaoyan Yang Yu Kang Yanling Fan Jingkuan Wei Zunpeng Liu Shaoxing Dai Hao Li Zifan Li Lizhu Xu Chu Chu Jing Qu Chenyang Si Weizhi Ji Guang-Hui Liu Chengzu Long Yuyu Niu | 2020 | Protein & Cell2020，11，11： | 11 |
| 8 | Identification and characterization of an ancient class of small RNAs enriched in serum associating with active infection显示文摘 | Yunfang Zhang Ying Zhang Junchao Shi He Zhang Zhonghong Cao Xuan Gao Wanhua Ren Yunna Ning Lina Ning Yujing Cao Yongchang Chen Weizhi Ji Zi-jiang Chen Qi Chen Enkui Duan | 2014 | Journal of Molecular Cell Biology2014，8，2： | 10 |
| 9 | Genome editing in large animals: current status and future prospects显示文摘Large animals(non-human primates,livestock and dogs)are playing important roles in biomedical research,and large livestock animals serve as important sources of meat and milk.The recently developed programmable DNA nucleases have revolutionized the generation of gene-modified large animals that are used for biological and biomedical research.In this review,we briefly introduce the recent advances in nuclease-meditated gene editing tools,and we outline these editing tools’applications in human disease modeling,regenerative medicine and agriculture.Additionally,we provide perspectives regarding the challenges and prospects of the new genome editing technology. | Jianguo Zhao Liangxue Lai Weizhi Ji Qi Zhou | 2019 | National Science Review2019，6，3： | 10 |
| 10 | Germline acquisition of Cas9/RNA-mediated gene modifications in monkeys显示文摘 | Yongchang Chen Yiqiang Cui Bin Shen Yuyu Niu Xiaoyang Zhao Lei Wang Jianying Wang Wei Li Qi Zhou Weizhi Ji Jiahao Sha Xingxu Huang | 2015 | Cell Research2015，25，2： | 8 |
| 11 | Channel Modeling and Performance Analysis for UAV Relay Systems显示文摘In this paper, a multi-hop relay channel model based on unmanned aerial vehicles(UAVs) is established by taking into account of the propagation loss, shadowing, and multi-path fading. Based on the proposed channel model, the cascaded propagation loss of relay link and the cascaded probability density function(PDF) of channel fading are derived. Moreover, the theoretical performance of the UAV-based relay system, i.e., the outage probability, bit error rate(BER), and channel capacity, is also analysed and derived. Simulation results show agreement with theoretical results for the hill, mountain, and sea scenarios, indicating the accuracy of both the simulations and derivations. | Xiaomin Chen Xujun Hu Qiuming Zhu Weizhi Zhong Bin Chen | 2018 | China Communications2018，15，12： | 8 |
| 12 | Global profiling of crotonylation on non-histone proteins显示文摘 | Weizhi Xu Junhu Wan Jun Zhan Xueying Li Huiying He Zhaomei Shi Hongquan Zhang | 2017 | Cell Research2017，27，7： | 7 |
| 13 | MmWave Beamforming for UAV Communications with Unstable Beam Pointing显示文摘Millimeter wave(mmWave) communications of unmanned aerial vehicles(UAVs) have drawn dramatic attentions for its flexibility on a variety of applications.Recently,channel tracking base on the spatial features has been proposed to solve the problem of beam misalignments due to the UAV navigation.However,unstable beam pointing caused by the non-ideal beam tracking environment may impact the performance of mmWave systems significantly.In this paper,an improved beamforming method is presented to overcome this shortcoming.Firstly,the effect of the beam deviation is analyzed through the establishment of the equivalent data rate.Then,combining the quantification of spatial angle and the improved orthogonal matching pursuit(OMP) algorithm,an optimized beam corresponding to the beam deviation is obtained.Simulation results show that the optimized beam of the proposed approach can effectively improve the spectral efficiency without improving the complexity when the beam pointing is unstable. | Weizhi Zhong Lei Xu Qiuming Zhu Xiaomin Chen Jianjiang Zhou | 2019 | China Communications2019，16，1： | 7 |
| 14 | Micro-droplet Digital Polymerase Chain Reaction and Real-Time Quantitative Polymerase Chain Reaction Technologies Provide Highly Sensitive and Accurate Detection of Zika Virus显示文摘The establishment of highly sensitive diagnostic methods is critical in the early diagnosis and control of Zika virus(ZIKV)and in preventing serious neurological complications of ZIKV infection. In this study, we established micro-droplet digital polymerase chain reaction(ddPCR) and real-time quantitative PCR(RT-qPCR) protocols for the detection of ZIKV based on the amplification of the NS5 gene. For the ZIKV standard plasmid, the RT-qPCR results showed that the cycle threshold(Ct) value was linear from 10~1 to 10~8 copy/l L, with a standard curve R^2 of 0.999 and amplification efficiency of 92.203%;however, a concentration as low as 1 copy/l L could not be detected. In comparison with RT-qPCR, the dd PCR method resulted in a linear range of 10~1–10~4 copy/l L and was able to detect concentrations as low as 1 copy/l L. Thus, for detecting ZIKV from clinical samples, RT-qPCR is a better choice for high-concentration samples(above 10~1 copy/l L),while ddPCR has excellent accuracy and sensitivity for low-concentration samples. These results indicate that the ddPCR method should be of considerable use in the early diagnosis, laboratory study, and monitoring of ZIKV. | Yuan Hui Zhiming Wu Zhiran Qin Li Zhu Junhe Liang Xujuan Li Hanmin Fu Shiyu Feng Jianhai Yu Xiaoen He Weizhi Lu Weiwei Xiao Qinghua Wu Bao Zhang Wei Zhao | 2018 | Virologica Sinica2018，33，3： | 6 |
| 15 | A nontrivial factor in determining current distribution in an ac HTS cable-proximity effect显示文摘A superconductor has zero resistance at the superconducting state. This unique property creates many exceptional phenomena, of which some are known and the others are not. Our experiments with multilayer high temperature superconductor (HTS) cable samples revealed a new phenomenon that alternating current had a tendency to flow in the inner and outer layers of the cables. We attribute the cause of this phenomenon to the electromagnetic interaction in an infinite electrical conductivity medium and term it 'super-proximity-effect'. This effect will greatly affect the performance of a multilayer superconducting cable and other superconducting devices which are involved with alternating current transportation. | XIN Ying,GONG WeiZhi & ZHANG JingYin Innopower Superconductor Cable Co.,Ltd.,7 E.Rongchang Rd.,Longsheng Industrial Park,Beijing Economic & Technological Development Area,Beijing 100176,China | 2010 | Science China(Technological Sciences)2010，53，4： | 6 |
| 16 | Transgenic Nonhuman Primate Models for Human Diseases:Approaches and Contributing Factors显示文摘非人类的首领(NHP ) 提供有力的试验性的模型学习人的开发,认知功能和骚乱以及复杂行为,因为他们到人的基因、生理的类似。因此, NHP 是为人的疾病的学习的适当模型,例如包括 Parkinson 的, Alzheimer 的并且亨廷顿的疾病的 neurodegenerative 疾病,它由于基因变化发生。然而,折磨人的如此的疾病不在 NHP 自然地发生。那么转基因的 NHP 需要被证实理解疾病病理和致病的病原学。把基因模型,比作啮齿类动物为人的疾病的转基因的 NHP 的产生是低效的,并且为亨廷顿的疾病的一个仅仅转基因的猴子模型被报导了。这评论为产生转基因的 NHP 学习人的疾病集中于潜在的途径和作出贡献的因素。 | Yongchang Chen | 2012 | Journal of Genetics and Genomics2012，39，6： | 5 |
| 17 | Pharmacokinetics and Biodegradation Performance of a Hydroxypropyl Chitosan Derivative显示文摘Hydroxypropyl chitosan(HP-chitosan) has been shown to have promising applications in a wide range of areas due to its biocompatibility, biodegradability and various biological activities, especially in the biomedical and pharmaceutical fields. However, it is not yet known about its pharmacokinetics and biodegradation performance, which are crucial for its clinical applications. In order to lay a foundation for its further applications and exploitations, here we carried out fluorescence intensity and GPC analyses to determine the pharmacokinetics mode of fluorescein isothiocyanate-labeled HP-chitosan(FITC-HP-chitosan) and its biodegradability. The results showed that after intraperitoneal administration at a dose of 10 mg per rat, FITC-HP-chitosan could be absorbed rapidly and distributed to liver, kidney and spleen through blood. It was indicated that FITC-HP-chitosan could be utilized effectively, and 88.47% of the FITC-HP-chitosan could be excreted by urine within 11 days with a molecular weight less than 10 k Da. Moreover, our data indicated that there was an obvious degradation process occurred in liver(< 10 k Da at 24 h). In summary, HP-chitosan has excellent bioavailability and biodegradability, suggesting the potential applications of hydroxypropyl-modified chitosan as materials in drug delivery, tissue engineering and biomedical area. | SHAO Kai HAN Baoqin DONG Wen SONG Fulai LIU Weizhi LIU Wanshun | 2015 | Journal of Ocean University of China2015，14，5： | 5 |
| 18 | Cu(I)-Catalyzed Three-Component Coupling of Trifluoromethyl Ketone N-Tosylhydrazones, Alkynes and Azides: Synthesis of Difluoromethylene Substituted 1,2,3-Triazoles显示文摘一 trifluoromethyl 酉同类 N-tosylhydrazones,炔属羟和叠氮化物的催化 CuI 的三部件的联合被开发了。reactionrepresents 存取 difluoromethylene 的一个直接方法代替了 1,2,3-triazoles。机械学地,反应跟随包含 Cu (I) triazolide 中介, Cu (I) carbene 形成,迁移的插入,和氟化物消除的形成的一条小径,这被建议了。Thetransformation 被温和条件,宽底层范围和高效率展示。 | Zhikun Zhang Qi Zhou Weizhi Yu Tianjiao Li Yan Zhang Jianbo Wang | 2017 | Chinese Journal of Chemistry2017，35，4： | 4 |
| 19 | A theranostic agent for cancer therapy and imaging in the second nearinfrared window显示文摘Thera no stic nano particles are integrated systems useful for simulta neous diag nosis and imaging guided delivery of therapeutic drugs, with wide ranging pote ntial applicati ons in the clinic. Here we developed a thera no stic nan oparticle (~24 nm size by dynamic light scatteri ng) p-FE-PTX-FA based on polymeric micelle encapsulating an organic dye (FE) fluorescing in the 1,000-1,700 nm second near-infrared (NIR-Ⅱ) window and an an ti-ca ncer drug paclitaxel. Folic acid (FA) was conjugated to the nan oparticles to afford specific binding to molecular folate receptors on muri ne breast can cer 4T1 tumor cells. In vivo, the nan oparticles accumulated in 4T1 tumor through both passive and active targeting effect. Under an 808 nm laser excitation, fluorescence detection above 1,300 nm afforded a large Stokes shift, allowing targeted molecular imaging tumor with high signal to background ratios, reaching a high tumor to normal tissue signal ratio (T/NT) of (20.0±2.3). Further, 4T1 tumors on mice were completed eradicated by paclitaxel released from p-FE-PTA-FA within 20 days of the first injection. Pharmacokinetics and histology studies indicated p-FE-PTX-FA had no obvious toxic side effects to major organs. This represented the first NIR-Ⅱ theranostic age nt developed. | Zhuoran Ma Hao Wan Weizhi Wang Xiaodong Zhang Takaaki Uno Qianglai Yang Jingying Yue Hongpeng Gao Yeteng Zhong Ye Tian Qinchao Sun Yongye Liang Hongjie Dai | 2019 | Nano Research2019，12，2： | 4 |
| 20 | Piperlongumine Inhibits Zika Virus Replication In vitro and Promotes Up-Regulation of HO-1 Expression, Suggesting An Implication of Oxidative Stress显示文摘Owing to the widespread distribution of mosquitoes capable of transmitting Zika virus, lack of clinical vaccines and treatments, and poor immunity of populations to new infectious diseases, Zika virus has become a global public health concern. Recent studies have found that Zika virus can continuously infect human brain microvascular endothelial cells.These cells are the primary components of the blood–brain barrier of the cerebral cortex, and further infection of brain tissue may cause severe damage such as encephalitis and fetal pituitary disease. The present study found that a biologically active base, piperlongumine(PL), inhibited Zika virus replication in human brain microvascular endothelial cells, Vero cells, and human umbilical vein endothelial cells. PL also significantly increased heme oxygenase-1(HO-1) gene expression, while silencing HO-1 expression and using the reactive oxygen species scavenger, N-acetylcysteine, attenuated the inhibitory effect of PL on Zika virus replication. These results suggest that PL induces oxidative stress in cells by increasing reactive oxygen species. This, in turn, induces an increase in HO-1 expression, thereby inhibiting Zika virus replication. These findings provide novel clues for drug research on the prevention and treatment of Zika virus. | Weizhi Lu Linjuan Shi Jing Gao Huimin Zhu Ying Hua Jintai Cai Xianbo Wu Chengsong Wan Wei Zhao Bao Zhang | 2021 | Virologica Sinica2021，36，3： | 4 |
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