|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Changes of chlorogenic acid content and its synthesis-associated genes expression in Xuehua pear fruit during development显示文摘According to synthetic pathway of plant chlorogenic acid(CGA),the expression patterns of genes encoding enzymes that are associated with CGA synthesis were studied in normally developed Xuehua pear fruit. The study demonstrated that CGA content in peel and flesh of Xuehua pear decreased as fruit development progressed,with a higher level in peel. The expression levels of PbPAL1,PbPAL2,PbC3H,PbC 4H,Pb4CL1,Pb4CL2,Pb4CL6,PbHCT1 and PbHCT3 genes decreased in fruit,which was consistent with the pattern of variation in CGA content. That indicated that these genes might be key genes for influencing fruit CGA synthesis in Xuehua pear. However,Pb4CL7 gene expression profile is not consistent with variation of CGA content,hence,it may not be a key gene involved in CGA synthesis. | HE Jin-gang CHENG Yu-dou GUAN Jun-feng GE Wen-ya ZHAO Zhe | 2017 | Journal of Integrative Agriculture2017，16，2： | 13 |
| 2 | Homocysteine, hypertension, and risks of cardiovascular events and all-cause death in the Chinese elderly population: a prospective study显示文摘BACKGROUND Increased homocysteine levels are associated with the risk of cardiovascular disease(CVD)and death.However,their prevention has not been effective in decreasing CVD risk.This study investigated the individual and combined associations of hyperhomocysteinemia and hypertension with incident CVD events and all-cause death in the Chinese elderly population without a history of CVD.METHODS This prospective study was conducted among 1,257 elderly participants(mean age:69 years).A questionnaire survey,physical examinations,and laboratory tests were conducted to collect baseline data.Hyperhomocysteinemia was defined as homocysteine level≥15μmol/L.H-type hypertension was defined as concomitant hypertension and hyperhomocysteinemia.Multivariate Cox regression analysis was used to evaluate individual and combined associations of hyperhomocysteinemia and hypertension with the risks of incident CVD events and all-cause death.RESULTS Over a median of 4.84-year follow-up,hyperhomocysteinemia was independently associated with incident CVD events and all-cause death.The hazard ratios(HRs)were 1.45(95%CI:1.01−2.08)for incident CVD events and 1.55(95%CI:1.04−2.30)for all-cause death.After adjustment for confounding factors,H-type hypertension had the highest HRs for incident CVD events and all-cause death.The fully adjusted HRs were 2.44 for incident CVD events(95%CI:1.28−4.65),2.07 for stroke events(95%CI:1.01−4.29),8.33 for coronary events(95%CI:1.10−63.11),and 2.31 for all-cause death(95%CI:1.15−4.62).CONCLUSIONS Hyperhomocysteinemia was an independent risk factor,and when accompanied by hypertension,it contrib-uted to incident CVD events and all-cause death in the Chinese elderly population without a history of CVD. | Zhong-Ying ZHANG Xiang GU Zhe TANG Shao-Chen GUAN Hong-Jun LIU Xiao-Guang WU Yan ZHAO Xiang-Hua FANG | 2021 | Journal of Geriatric Cardiology2021，18，10： | 10 |
| 3 | Biodegradation,hemocompatibility and covalent bonding mechanism of electrografting polyethylacrylate coating on Mg alloy for cardiovascular stent显示文摘Organic coatings are the most widely employed approach for the promotion of corrosion resistance of magnesium(Mg)alloys.Unfortunately,traditional organic coatings are weakly bonded to Mg substrates due to physical adsorption.Herein,a polyethylacrylate(PEA)coating was fabricated on Mg-Zn-YNd alloy via electro-grafting.The surface structure and chemical composition were characterized by means of scanning electron microscope(SEM),energy dispersive X-ray spectroscopy(EDS),atomic force microscope(AFM)and Fourier transform infrared(FTIR)as well as time of flight-secondary ion mass spectrometer(To F-SIMS).The results showed that the surface roughness of PEA coating was dominated by scan rate;while the coverage and integrity of PEA coating were mainly affected by the monomer concentration and sweep circles.To F-SIMS results indicated that PEA coating was wholly covered on Mg alloy,and the presence of C2H3Mg-fragment confirmed the covalent bond between PEA coating and Mg alloy.In addition,DFT calculation results of the adsorption of EA molecules with Mg substrate agree well with the experimental phenomena and observation,suggesting that the electrons in 3s orbit of Mg atoms and 2pz orbit of C1 atom participated in the formation of covalent bond between PEA coating and Mg substrate.Potentiodynamic polarization curves and immersion test demonstrated that the PEA coatings could effectively enhance the corrosion resistance of Mg alloy.The platelet adhesion results designated that platelets were barely visible on PEA coating,which implied that PEA coating could effectively prevent the thrombosis and coagulation of platelets.PEA coating might be a promising candidate coating of Mg alloy for cardiovascular stent. | Yong-Xin Yang Zhe Fang Yi-Hao Liu Ya-Chen Hou Li-Guo Wang Yi-Fan Zhou Shi-Jie Zhu Rong-Chang Zeng Yu-Feng Zheng Shao-Kang Guan | 2020 | Journal of Materials Science & Technology2020，42，11： | 9 |
| 4 | Sensitivity-enhanced surface plasmon resonance sensor utilizing a tungsten disulfide (WS_2) nanosheets overlayer显示文摘Tungsten disulfide(WS_2), as a representative layered transition metal dichalcogenide(TMDC) material, possesses important potential for applications in highly sensitive sensors. Here, a sensitivity-enhanced surface plasmon resonance(SPR) sensor with a metal film modified by an overlayer of WS_2 nanosheets is proposed and demonstrated. The SPR sensitivity is related to the thickness of the WS_2 overlayer, which can be tailored by coating a WS_2 ethanol suspension with different concentrations or by the number of times of repeated post-coating.Benefitting from its large surface area, high refractive index, and unique optoelectronic properties, the WS_2 nanosheet overlayer coated on the gold film significantly improves the sensing sensitivity. The highest sensitivity(up to 2459.3 nm∕RIU) in the experiment is achieved by coating the WS_2 suspension once. Compared to the case without a WS_2 overlayer, this result shows a sensitivity enhancement of 26.6%. The influence of the WS_2 nanosheet overlayer on the sensing performance improvement is analyzed and discussed. Moreover, the proposed WS_2 SPR sensor has a linear correlation coefficient of 99.76% in refractive index range of 1.333 to 1.360. Besides sensitivity enhancement, the WS_2 nanosheet overlayer is able to show additional advantages, such as protection of metal film from oxidation, tunability of the resonance wavelength region, biocompatibility, capability of vapor,and gas sensing. | HAO WANG HUI ZHANG JIANGLI DONG SHIQI HU WENGUO ZHU WENTAO QIU HUIHUI LU JIANHUI YU HEYUAN GUAN SHECHENG GAO ZHAOHUI LI WEIPING LIU MIAO HE JUN ZHANG ZHE CHEN YUNHAN LUO | 2018 | Photonics Research2018，6，6： | 8 |
| 5 | Carbon Monoxide Promotes Lateral Root Formation in Rapeseed显示文摘一氧化碳(公司) ,无臬、无味、无色的气体,最近证明了在哺乳动物的房间是活跃的一份重要简历或信号分子,主要由氮的氧化物与它的效果调停了(没有) 。在现在的报告,我们证明外长的公司导致侧根(LR ) 形成,一个没有依赖的过程。到油菜籽的 CO 施主血色素的管理(Brassicanapus L。Yangyou 6 ) 为 3 天的幼苗, dose-dependently 支持了全部的长度和 numberofLRs。这些回答也被看见跟随不同浸透的集中的气体的公司水溶液的应用程序。而且,幼苗上的公司的行动充分被颠倒 CO scavenger 血红素(Hb ) 或公司特定的合成禁止者锌 protoporphyrin-IX (ZnPPIX ) 什么时候被增加。有趣地,它的特定的scavenger2-( 4-carboxyphenyl ) -4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide 钾腌的内长的 NO using ( cPTIO )的弄空或氮的氧化物 synthase ( NOS )禁止者 N^G-nitro-L-arginine 甲基酉旨(L名字),导致了完全的废除 ofLR 发展,说明一个重要角色为内长不在 LR 上的公司的行动形成。然而,由 200 亩 mol/L 钠 nitroprusside (SNP ) 的 LR 开发的正式就职,一没有施主,没被 ZnPPIX 的存在或缺席影响。而且,使用一条解剖途径与与没有特定的 fluorophore4,5-diaminofluorescein diacetate 扫描共焦的显微镜学的激光结合了,我们观察到血色素和 SNP 与控制样品增加了 NOreleasecompared 并且 NO 信号主要在 LR primordia (LRP ) 被散布,特别在 36 h 处理以后。LRP 被发现在控制有类似的形态学,对待 SNP 、对待血色素的幼苗。同样, NO 的改进由在 36 h 的公司的信号是 cPTIO, L 名字, ZnPPIX 和 Hb 的增加熄灭的差别。相反,正式就职不由 SNP 引起了没被 ZnPPIX 的申请影响。因此,我们进一步推断公司导致 LR 形成可能调停了由没有 / 没有小径并且可以不 COsignaling 下游地行动,它也被显示了发生在动物。 | Ze-Yu Cao Wei Xuan Zhao-Yang Liu Xin-Na Li Nan Zhao Peng Xu Zhe Wang Rong-Zhan Guan Wen-Biao Shen | 2007 | Journal of Integrative Plant Biology2007，49，7： | 7 |
| 6 | Seasonal variations in the concentration and removal of nonylphenol ethoxylates from the wastewater of a sewage treatment plant显示文摘In this study, we investigated the occurrence and fate of nonylphenol(NP), nonylphenol monoethoxylate(NP1EO) and nonylphenol diethoxylate(NP2EO) in a full scale sewage treatment plant, which applied an Anaerobic/Oxic process. Concentrations of NP, NP1 EO and NP2 EO in the wastewater were measured during the period spanning a whole year.The results showed remarkable seasonal variation in the concentrations of the compounds.The NPn EO compounds were most abundant in winter, with the total concentrations of influent NP, NP1 EO and NP2 EO ranging from 3900 to 7000 ng/L, 4000 to 4800 ng/L and 5200 to 7200 ng/L, respectively. Regarding the total removal efficiencies of the three types of short-chain NPn EO compounds, different trends were exhibited according to different seasons. The average removal efficiency of NP for the different seasons ranked as follows:winter > summer > autumn > spring; NP2 EO concentrations decreased as follows: summer >autumn > winter > spring, while NP1 EO concentrations reduced according to: spring >summer > autumn > winter. We also investigated the contribution ratio of individual treatment units in the A/O process, with the findings suggesting that the anaerobic treatment unit plays an important role in the elimination of short-chain NPn EOs from the wastewater. | Dawen Gao Zhe Li Junxue Guan Hong Liang | 2017 | Journal of Environmental Sciences2017，29，4： | 6 |
| 7 | Genetic analysis and preimplantation genetic diagnosis of Chinese Marfan syndrome patients显示文摘Marfan syndrome (MFS)(OMIM 154700) is a relatively common autosomal dominant genetic disease that causes skeletal, ocular, and cardiovascular defects and was first described by a French pediatrician in 1896 (Bitterman and Sponseller, 2017). Its prevalence rate is 1/3000—1/5000, and more than 25% of cases are sporadic (Chiu et al., 2014). Studies have shown that about 90% of MFS is caused by variants in the fibrillin-1 gene (FBN1, OMIM 134797). FBN1, located on chromosome 15q21.1, encodes a macromolecular glycoprotein-fibrin 1, which aggregates to form microfibers in the extracellular matrix and distributes in various human connective tissues, such as periosteum, vessel wall, and crystal suspensor ligament. Variants in FNB1 have been reported in 65 exons, but the relationship between genotype and phenotype remains rather unclear (Sakai et al., 2016). Studies have also shown that patients with MFS and similar diseases may have variants in other related genes such as members of the transforming growth factor beta receptor (TGFBR) family (Mizuguchi et al., 2004;Sakai et al., 2006;Bolar et al., 2012;De Cario et al., 2018). For better prevention and treatment of MFS as well as for suspected MFS patients, there is a strong need for efficient genetic testing for early diagnosis and differential diagnoses of patients with related phenotypes (Aubart et al., 2018). | Meng Qin Xiaohui Zhu Zhe Zhang Xuemin Li Zhiqiang Yan Yuqian Wang Shuo Guan Yihua He Wenxin Zhang Liying Yan Jie Qiao Xu Zhi | 2019 | Journal of Genetics and Genomics2019，46，6： | 6 |
| 8 | BPI_(700)-Fc∴1_(700) chimeric gene expression and its protective effect in a mice model of the lethal E. coli infection显示文摘Background Infections caused by gram-negative bacteria (GNB) often lead to high mortality in common clinical settings. The effect of traditional antibiotic therapy is hindered by drug-resistant bacteria and unneutralizable endotoxin. Few effective methods can protect high risk patients from bacterial infection. This study explored the protection of adeno-associated virus 2 (AAV2)-bacteriacidal permeability increasing protein 700 (BPI700) -fragment crystallizable gamma one 700 (Fc(1700) chimeric gene transferred mice against the minimal lethal dose (MLD) of E.coli and application of gene therapy for bacterial infection . Methods After AAV2-BPI700-Fc(1700 virus transfection,dot blotting and Western blotting were used to detect the target gene products in Chinese hamster ovary-K1 cells (CHO-K1cells). Reverse transcription-polymerase chain reaction and immunohistochemical assay were carried out to show the target gene expression in mice. Modified BPI-enzyme linked immunosorbent assay was used to identify the target gene products in murine serum. The protection of BPI700-Fc(1700 gene transferred mice was examined by survival rate after MLD E. coli challenge. Colony forming unit (CFU) count, limulus amebocyte lysate kit and cytokine kit were used to quantify the bacteria, the level of endotoxin, and proinflammatory cytokine. Results BPI1-199-Fc(1 protein was identified in the CHO-K1 cell culture supernatant, injected muscles and serum of the gene transferred mice. After MLD E. coli challenge, the survival rate of AAV2-BPI700-Fc(1700 gene transferred mice (36.7%) was significantly higher than that of AAV2-enhanced green fluorescent protein (AAV2- EGFP) gene transferred mice (3.3%) and PBS control mice (5.6%). The survival rate of AAV2-BPI700-Fc(1700 gene transferred mice treated with cefuroxime sodium was 65.0%. The bacterium number in main viscera, the levels of endotoxin and proinflammatory cytokine (tumor necrcsis factor-α and interleukin-1β) in serum of the AAV2-BPI700-Fc(1700 gene transferred mice were markedly lower than that of PBS control mice (P<0.01). Conclusions AAV2-BPI700-Fc(1700 gene transferred mice can resist MLD E. coli infection through expressing BPI1-199-Fc(1 protein. Our findings suggested that AAV2 mediated BPI700-Fc(1700 gene delivery could be used for protection and treatment of clinical GNB infection in high-risk individuals. | KONG Qing-li GUAN Yuan-zhi JING Xue-fang LI Chen GUO Xiang-hua LU Zhe AN Yun-qing | 2006 | Chinese Medical Journal2006，，6： | 5 |
| 9 | Rational development of a human antibody cocktail that deploys multiple functions to confer Pan-SARS-CoVs protection显示文摘Structural principles underlying the composition and synergistic mechanisms of protective monoclonal antibody cocktails are poorly defined.Here,we exploited antibody cooperativity to develop a therapeutic antibody cocktail against SARS-CoV-2.On the basis of our previously identified humanized cross-neutralizing antibody H014,we systematically an a lyzed a fully human n aive antibody library and rationally identified a potent neutralizing antibody partner,P17,which confers effective protection in animal model.Cryo-EM studies dissected the nature of the P17 epitope,which is SARS-CoV-2 specific and distinctly different from that of HOI4.High-resolution structure of the SARS-CoV-2 spike in complex with HOM and P17,together with functional investigations revealed that in a two-antibody cocktail,synergistic neutralization was achieved by S1 shielding and conformational locking,thereby blocking receptor attachment and viral membrane fusion,conferring high potency as well as robustness against viral mutation escape.Furthermore,cluster analysis identified a hypothetical 3rd antibody partner for further reinforcing the cocktail as pan-SARS-CoVs therapeutics. | Hangping Yao Yao Sun Yong-Qiang Deng Nan Wang Yongcong Tan Na-Na Zhang Xiao-Feng Li Chao Kong Yan-Peng Xuc Qi Chen Tian-Shu Cao Hui Zhao Xintian Yan Lei Cao Zhe Lv Dandan Zhu Rui Feng Nanping Wu Wenhai Zhang Yuhao Hu Keda Chen Rong-Rong Zhang Qingyu Lv Shihui Sun Yunhua Zhou Run Yan Guan Yang Xinglu Chanjuan Liu Xiangyun Lu Linfang Cheng Hongying Qiu Xing-Yao Huang Tianhao Weng Danrong Shi Weidong Jiang Junbin Shao Lei Wang Jie Zhang Tao Jiang Guojun Lang Cheng-Feng Qinc Lanjuan Li Xiangxi Wang | 2021 | Cell Research2021，31，1： | 5 |
| 10 | The Lantian biota:A new window onto the origin and early evolution of multicellular organisms显示文摘The Lantian biota at the Lantian Town of Xiuning County,Anhui Province,is preserved in black shales of the Ediacaran Lantian Formation.It yields some of the oldest known complex macroorganisms,including fan-shaped seaweeds and possible animal fossils with tentacles and intestinal-like structures reminiscent of modern coelenterates and bilaterians.The Lantian Lagerst tte sheds new light on the origin and early evolution of multicellular organisms in relatively quiet and deep environments soon after the Neoproterozoic Marinoan glaciation.The morphological complexity and diversity of early multicellular organisms may be closely related to sexual reproduction and alternation of generations.The fluctuation of oceanic redox conditions during this period may have played a role in the ecology and preservation of the Lantian biota. | YUAN XunLai CHEN Zhe XIAO ShuHai WAN Bin GUAN ChengGuo WANG Wei ZHOU ChuanMing HUA Hong | 2013 | Science China(Technological Sciences)2013，56，3： | 5 |
| 11 | Expression of pyruvate dehydrogenase is an independent prognostic marker in gastric cancer显示文摘AIM:To investigate the expression and prognostic role of pyruvate dehydrogenase(PDH) in gastric cancer(GC).METHODS:This study included 265 patients(194 male,71 female,mean age 59 years(range,29-81 years) with GC who underwent curative surgery at the First Affiliated Hospital of China Medical University from January 2006 to May 2007.All patients were followed up for more than 5 years.Patient-derived paraffin embedded GC specimens were collected for tissue microarrays(TMAs).We examined PDH expression by immunohistochemistry in TMAs containing tumor tissue and matched nonneoplastic mucosa.Immunoreactivity was evaluated independently by two researchers.Overall survival(OS) rates were determined using the Kaplan-Meier estimator.Correlations with other clinicopathologic factors were evaluated by two-tailed χ2 tests or a two-tailed t-test.The Cox proportional-hazard model was used in univariate analysis and multivariate analysis to identify factors significantly correlated with prognosis.RESULTS:Immunohistochemistry showed that 35.47% of total cancer tissue specimens had cytoplasmic PDH staining.PDH expression was much higher in normal mucosa specimens(75.09%;P = 0.001).PDH expression was correlated with Lauren grade(70.77% in intestinal type vs 40.0% in diffuse type;P = 0.001),lymph node metastasis(65.43% with no metastasis vs 51.09% with metastasis;P = 0.033),lymphatic invasion(61.62% with no invasion vs 38.81% with invasion;P = 0.002),histologic subtypes(70.77% in intestinal type vs 40.0% in diffuse type;P = 0.001) and tumor-node-metastasis(TNM) stage(39% in poorly differentiated vs 65.91% in well differentiated and 67.11% in moderately differentiated;P = 0.001) in GC.PDH expression in cancer tissue was significantly associated with higher OS(P < 0.001).The multivariate analysis adjusted for age,Lauren classification,TNM stage,lymph node metastasis,histological type,tumor size,depth of invasion and lymphatic invasion showed that the PDH expression in GC was an independent prognostic factor for higher OS(HR = 0.608,95%CI:0.504-0.734,P < 0.001).CONCLUSION:Our study indicated that PDH expression is an independent prognostic factor in GC patients and that positive expression of PDH may be predictive of favorable outcomes. | Xu-Ren Sun Zhe Sun Zhi Zhu Hai-Xia Guan Chen-Yan Li Jun-Yan Zhang Yi-Ning Zhang Huan Zhou Hui-Jing Zhang Hui-Mian Xu Ming-Jun Sun | 2015 | World Journal of Gastroenterology2015，21，17： | 4 |
| 12 | A preliminary analysis and discussion of the condominium building collapse in surfside,Florida,US,June 24,2021显示文摘On June 24,2021,a 40-year-old reinforced concrete flat plate structure building in Miami suffered a sudden partial collapse.This study analyzed the overall performance and key components of the collapsed building based on the building design codes(ACI-318 and GB 50010).Punching shear and post-punching performances of typical slabcolumn joints are also studied through the refined finite element analysis.The collapse process was simulated and visualized using a physics engine.By way of these analyses,weak design points of the collapsed building are highlighted.The differences between the reinforcement detailing of the collapsed building and the requirements of the current Chinese code are discussed,together with a comparison of the punching shear and post-punching performances.The simulated collapse procedure and debris distribution are compared with the actual collapse scenes. | Xinzheng LU Hong GUAN Hailin SUN Yi LI Zhe ZHENG Yifan FEI Zhi YANG Lingxiao ZUO | 2021 | Frontiers of Structural and Civil Engineering2021，15，5： | 4 |
| 13 | Effects of Ginkgo biloba extract EGb761 on neural differentiation of stem cells offer new hope for neurological disease treatment显示文摘Stem cell transplantation has brought new hope for the treatment of neurological diseases.The key to stem cell therapy lies in inducing the specific differentiation of stem cells into nerve cells.Because the differentiation of stem cells in vitro and in vivo is affected by multiple factors,the final differentiation outcome is strongly associated with the microenvironment in which the stem cells are located.Accordingly,the optimal microenvironment for inducing stem cell differentiation is a hot topic.EGb761 is extracted from the leaves of the Ginkgo biloba tree.It is used worldwide and is becoming one of the focuses of stem cell research.Studies have shown that EGb761 can antagonize oxygen free radicals,stabilize cell membranes,promote neurogenesis and synaptogenesis,increase the level of brain-derived neurotrophic factors,and replicate the environment required during the differentiation of stem cells into nerve cells.This offers the possibility of using EGb761 to induce the differentiation of stem cells,facilitating stem cell transplantation.To provide a comprehensive reference for the future application of EGb761 in stem cell therapy,we reviewed studies investigating the influence of EGb761 on stem cells.These started with the composition and neuropharmacology of EGb761,and eventually led to the finding that EGb761 and some of its important components play important roles in the differentiation of stem cells and the protection of a beneficial microenvironment for stem cell transplantation. | Chao Ren Yong-Qiang Ji Hong Liu Zhe Wang Jia-Hui Wang Cai-Yi Zhang Li-Na Guan Pei-Yuan Yin | 2019 | Neural Regeneration Research2019，14，7： | 4 |
| 14 | Emerging role of cystic fibrosis transmembrane conductance regulator- an epithelial chloride channel in gastrointestinal cancers显示文摘Cystic fibrosis transmembrane conductance regulator(CFTR), a glycoprotein with 1480 amino acids, has been well established as a chloride channel mainly expressed in the epithelial cells of various tissues and organs such as lungs, sweat glands, gastrointestinal system, and reproductive organs. Although defective CFTR leads to cystic fibrosis, a common genetic disorder in the Caucasian population, there is accumulating evidence that suggests a novel role of CFTR in various cancers, especially in gastroenterological cancers, such as pancreatic cancer and colon cancer. In this review, we summarize the emerging findings that link CFTR with various cancers, with focus on the association between CFTR defects and gastrointestinal cancers as well as the underlying mechanisms. Further study of CFTR in cancer biology may help pave a new way for the diagnosis and treatment of gastrointestinal cancers. | Yuning Hou Xiaoqing Guan Zhe Yang Chunying Li | 2016 | World Journal of Gastrointestinal Oncology2016，8，3： | 3 |
| 15 | Protection of Immuno-Compromised Mice from Lethal Infection of Klebsiella pneumonia by rAAV2-BPI23-Fcγ1 Gene Transfer显示文摘In previous research, chimerical BPI23-Fcγ1 gene which consisted of human bactericidal/permeability increasing protein (BPI) gene of encoding the functional N terminus (amino acid residues 1 to 199) of human BPI and Fcγ1 gene of encoding the Fc segment of human immunoglobulin G1 was successfully reconstructed within a recombinant adeno-associated virus serotype 2 (rAAV2) vector as rAAV2-BPI23-Fcγ1. Here, to evaluate the potentiality of applying gene therapy to gram negative bacterial (GNB) infection in high-risk patients, we investigated protection of immuno-compromised mice and immunocompetent mice from challenge with minimal lethal dose (MLD) Klebsiella pneumonia infection after rAAV2-BPI23-Fcγ1 gene transferred. The results showed that the survival rate of rAAV2-BPI23-Fcγ1 transferred immunocompetent mice as well as immuno-compromised mice (40.0% and 44.4%, respectively) were significantly higher than that of corresponding control mice (6.7% and 4.4%, respectively); the bacteria counting, level of endotoxin and proinflammatory cytokines in the rAAV2-BPI23- Fcγ1 transferred immuno-compromised mice were markedly lower than that of rAAV2-EGFP and rAAV2-Null transferred immuno-compromised mice. Our data suggest that rAAV2-BPI23-Fcγ1 gene transferring offered immuno-compromised mice with resistance against GNB infection, so it is quite potential in preventing GNB infection of clinical high-risk patients. | Jing Li Qingli Kong Zhe Lv Yuanzhi Guan Yong Qiu Chen Li Mingjie Sun Zhenlong Liu Yunqing An | 2008 | Cellular & Molecular Immunology2008，5，6： | 2 |
| 16 | Progress of Fengyun Meteorological Satellites Since 2020显示文摘China’s efforts to develop Fengyun meteorological satellites have made major strides over the past 50 years,with the polar and geostationary meteorological satellite series achieving continuously stable operation to persistently provide data and product services globally.By the end of 2021,19 Chinese self-developed Fengyun meteorological satellites have been launched successfully.Seven of them are in operation at present,the data and products are widely applied to weather analysis,numerical weather forecasting and climate prediction,as well as environment and disaster monitoring.Since the last COSPAR report,FY-4B,the first new-generation operational geostationary satellite,and FY-3E,the first early-morning orbit satellite in China’s polar-orbiting meteorological satellite family have been launched in 2021.The characteristics of the two latest satellites and the instruments onboard are addressed in this report.The status of current Fengyun Satellites,product and data service and international cooperation and supporting activities has been introduced as well. | ZHANG Peng XU Zhe GUAN Min XIE Lizi XIAN Di LIU Chang | 2022 | 空间科学学报2022，42，4： | 2 |
| 17 | Mis-estimation of coronary lesions and rectification by SYNTAX score feedback for coronary revascularization appropriateness显示文摘Background::Imprecise interpretation of coronary angiograms was reported and resulted in inappropriate revascularization.Synergy Between Percutaneous Coronary Intervention with Taxus and Cardiac Surgery(SYNTAX)score is a comprehensive system to evaluate the complexity of the overall lesions.We hypothesized that a real-time SYNTAX score feedback from image analysts may rectify the mis-estimation and improve revascularization appropriateness in patients with stable coronary artery disease(CAD).Methods::In this single-center,historical control study,patients with stable CAD with coronary lesion stenosis≥50%were consecutively recruited.During the control period,SYNTAX scores were calculated by treating cardiologists.During the intervention period,SYNTAX scores were calculated by image analysts immediately after coronary angiography and were provided to cardiologists in real-time to aid decision-making.The primary outcome was revascularization deemed inappropriate by Chinese appropriate use criteria for coronary revascularization.Results::A total of 3245 patients were enrolled and assigned to the control group(08/2016-03/2017,n=1525)or the intervention group(03/2017-09/2017,n=1720).For SYNTAX score tertiles,17.9%patients were overestimated and 4.3%were underestimated by cardiologists in the control group.After adjustment,inappropriate revascularization significantly decreased in the intervention group compared with the control group(adjusted odds ratio[OR]:0.83;95%confidence interval[CI]:0.73-0.95;P=0.007).Both inappropriate percutaneous coronary intervention(adjusted OR:0.82;95%CI:0.74-0.92;P<0.001)and percutaneous coronary intervention utilization(adjusted OR:0.88;95%CI:0.79-0.98;P=0.016)decreased significantly in the intervention group.There was no significant difference in 1-year adverse cardiac events between the control group and the intervention group.Conclusions::Real-time SYNTAX score feedback significantly reduced inappropriate coronary revascularization in stable patients with CAD.Clinical trial registration::Nos.NCT03068858 and NCT02880605;http://gffzz1caf53c915c04334s5b9f6k9nv56x6u96.ffgz.tsg.suse.edu.cn. | Shen Lin Heng Zhang Si-Peng Chen Chen-Fei Rao Fan Wu Fa-Jun Zhou Yun Wang Hong-Bing Yan Ke-Fei Dou Yong-Jian Wu Yi-Da Tang Li-Hua Xie Chang-Dong Guan Bo Xu Zhe Zheng | 2020 | Chinese Medical Journal2020，，11： | 2 |
| 18 | Influence of Soy Isoflavone on Lindane Cumulant in Sprague-Dawley Rats显示文摘Female Sprague-Dawley rats weighing 60-80 g were given different dosages of soy isoflavones and/or lindane for four weeks.Soy isoflavones was added in feed and lindane was given by oral gavage.We found that soy isoflavones could reduce the level of lindane in rat’s serum and brain,but might cause the uterus hyperplasia.Lindane could inhibit the effect of soy isoflavones on uterus and | YANG Yang ZHANG Yun Bo SHENG Wei FU Zheng Hai WANG Zhe WANG Xiao GUAN Si Yuan NA Xiao Lin | 2014 | Biomedical and Environmental Sciences2014，27，8： | 2 |
| 19 | On the technologies of Hα imaging spectrograph for the CHASE mission显示文摘The Hα imaging spectrograph(HIS) is the scientific payload of the first solar space mission, the Chinese Hα solar explorer(CHASE), supported by the China National Space Administration(CNSA). The CHASE/HIS achieves, for the first time in space, Hα spectroscopic observations with high spectral and temporal resolutions. Separate channels for the raster scanning mode(RSM) and continuum imaging mode(CIM) are integrated into one, and a highly integrated design is achieved through multiple folding of the optical path and ultra-light miniaturized components. The design of HIS implements a number of key technologies such as high-precision scanning of the optical field of view(FOV), high-precision integrated manufacturing inspection, a large-tolerance pre-filter window, and full-link solar radiation calibration. The HIS instrument has a pixel spectral resolution of 0.024 ? and can complete a full-Sun scanning within 46 s. | Qiang Liu Hongjiang Tao Changzheng Chen Chengshan Han Zhe Chen Gui Mei Liang Yang Qinglong Hu Hongwei Xin Xiansheng Li Hongyu Guan Donglin Xue Mingchao Zhu Changhong Hu Qinghua Ha Yukun He Cheng Fang Chuan Li Zhen Li | 2022 | Science China(Physics,Mechanics & Astronomy)2022，65，8： | 2 |
| 20 | Genetic diversity center of cultivated soybean(Glycine max) in China——New insight and evidence for the diversity center of Chinese cultivated soybean显示文摘Information on the center of genetic diversity of soybean(Glycine max) will be helpful not only for designing efficient strategies for breeding programs, but also for understanding the domestication and origin of this species. Here, we describe an analysis of genetic diversity based on simple-sequence repeat(SSR) variations within a core collection of 2 111 accessions of Chinese soybean landraces. Prior to the diversity assessment, the geographic origin of each accession was mapped. The map was then divided into grids each 2.5° in latitude and 5° in longitude. We found two regions that had higher number of alleles(NA) and greater polymorphic information content(PIC) values than the others. These regions are adjacently located within grid position of 30°–35°N×105°–110°E, which includes the valley of the middle and lower reaches of the Wei River, and the valley of the upper reaches of the Hanjiang River. It was also observed that in many regions, genetic diversity decreased with the increase in distance from the center. Another region, in northern Hebei Province(115°–120°E×40°–42.5°N), was observed having higher diversity than any surrounding regions, indicating that this is a sub-center of soybean diversity. Based on the presented results, the domestication and origin of soybean are also discussed. | WANG Li-xia LIN Fan-yun LI Lin-hai LI Wei YAN Zhe LUAN Wei-jiang PIAO Ri-hua GUAN Yuan NING Xue-cheng ZHU Li MA Yan-song DONG Zhi-min ZHANG Hai-yan ZHANG Yue-qiang GUAN Rong-xia LI Ying-hui LIU Zhang-xiong CHANG Ru-zhen QIU Li-juan | 2016 | Journal of Integrative Agriculture2016，15，11： | 2 |
您所在的机构暂未开通此部分的功能，请联系管理员