|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Derivation of Germline Competent Rat Embryonic Stem Cells from DA Rats显示文摘The laboratory rat was one of the earliest mammalian species for scientific research and used as animal disease models in physiology,toxicology,behavior,immunology,and tumor-biology for over 150 years(Jacob,1999).However,rat lags far behind mouse in generating human disease models and functional genomic studies because of the lack of authentic rat embryonic stem(ES) cells(Voigt and Serikawa, 2009),whereas the first mouse ES cell line was established in 1981(Evans and Kaufman,1981).By combining two or three kinase inhibitors which target GSK3,MEK and FGF signaling pathways in serum-free N2B27 medium,germline competent rat ES cells were first derived in 2008(Buehr et al.,2008; | Tianda Li Xiao-Yang Zhao Fei Teng Xin Li Minggui Jiang Wei Li Xuepeng Wang Jialiang Wang Lei Liu Zhonghua Liu Liu Wang Qi Zhou | 2012 | Journal of Genetics and Genomics2012，39，11： | 3 |
| 2 | Generation of Tripotent Neural Progenitor Cells from Rat Embryonic Stem Cells显示文摘老鼠是为药理学和生理的研究的一个珍贵模型。胚胎的茎(物件) 房间衬里的 Germline 能干的老鼠成功地被建立了,维持物件细胞的自我更新的、无差别的状态的分子的网络也好揭开。然而,很少对区别策略和这些真老鼠 pluripotent 干细胞怎么产生特定的房间类型的内在的机制被知道。这研究的目的是调查物件房间的神经区别能力。借助于一个修改过程基于 激活mitogen 的蛋白质 kinase ( MAPK )和肝糖 synthase kinase 的以前的出版物联合 3 ( GSK3 )禁止者(二个禁止者, 2i )与喂调节中等,我们成功地从物件房间获得了高质量的老鼠胚胎植物或动物身体(美国南北战争时南军士兵)然后区分了他们到 tripotent 神经祖先。这些物件导出房间的神经祖先房间(rNPCs ) 能够自我更新并且产生所有三个神经的系,包括的星形细胞, oligodendrocytes,和神经原。而且,这些导出房间的神经原染色了的物件为 -aminobutyric 酸(伽马氨基丁酸) 和酷氨酸 hydroxylase (TH ) 积极。在摘要,我们为区分物件房间到 tripotent 开发一个试验性的系统神经祖先,它可以为学习象 Parkinson 的疾病和老鼠神经系统的开发那样的许多 neurodegenerative 混乱的致病为药理学测试和一个珍贵平台提供一个强大的工具。 | Zhenkun Wang Chao Sheng Tianda Li Fei Teng Lisi Sang Fenglin Cao Ziwei Wang Wanwan Zhu Wei Li Xiaoyang Zhao Zhonghua Liu Liu Wang Qi Zhou | 2012 | Journal of Genetics and Genomics2012，39，12： | 2 |
| 3 | 24-h residual urine volume at hemodialysis initiation: A possible predictor for acute ischemic stroke incurrence in hemodialyis patients显示文摘 | Yuemei Chen Hong Liu Jianzhou Zou Yuxing Ge Jie Teng ShaoWei Xu WenLv Lv Zhonghua Liu Yan Xu XueSen Cao Bo Shen XiaoQiang Ding | 2012 | Clinical Neurology and Neurosurgery2012，，： | 1 |
| 4 | HPLC Fingerprint Chromatogram of Tetracera asiatica Produced in Guangxi显示文摘[Objectives] To establish fingerprint chromatogram of Tetracera asiatica and provide basis for controlling and assessing the quality of Tetracera asiatica.[Methods] The high-performance liquid chromatography( HPLC) method was used. Phenomenex C18( 5 μm,4. 6 ×250 mm),acetonitrile-0. 1% phosphoric acid water,gradient elution,flow rate 1 m L/min,detection wavelength 214 nm,and column temperature 25 ℃. The fingerprint chromatogram of 10 batches of samples of Tetracera asiatica was determined for similarity comparison. [Results] It established HPLC fingerprint chromatogram of Tetracera asiatica produced in Guangxi,determined a total of 13 common fingerprint peaks. The similarity of 10 batches of Tetracera asiatica was > 0. 9.[Conclusions] The method is rapid and accurate and can provide references for assessing the quality of Tetracera asiatica. | Fangfang DA Zhonghua DAI Hua ZHU Jianbei TENG | 2017 | Medicinal Plant2017，8，4： | 1 |
| 5 | An interval finite element method for electromagnetic problems with spatially uncertain parameters显示文摘During the manufacturing process of dielectric materials used in electromagnetic engineering, the electromagnetic parameters are often spatially uncertain due to the processing technology, environmental temperature, personal operations, etc. Traditionally,the random field model can be used to measure the spatial uncertainties, but its construction requires a large number of samples.On the contrary, the interval field model only needs the upper and lower bounds of the spatially uncertain parameters, which requires much less samples and furthermore is easy to understand and use for engineers. Therefore, in this paper, the interval field model is introduced to describe the spatial uncertainties of dielectric materials, and then an interval finite element method(IFEM) is proposed to calculate the upper and lower bounds of electromagnetic responses. Firstly, the interval field of the dielectric material is represented by the interval K-L expansion and inserted into the scalar Helmholtz wave equations, and thus the interval equilibrium equations are constructed according to the node-based finite element method. Secondly, a perturbation interval finite element method is developed for calculating the upper and lower bounds of electromagnetic responses such as the electric strength and magnetic strength. Finally, the effectiveness of the proposed method is verified by three numerical examples. | WANG ZhongHua JIANG Chao NI BingYu WANG CongSi ZHONG JianFeng FANG Teng | 2020 | Science China(Technological Sciences)2020，63，1： | 1 |
| 6 | Water extract of the fungi from Fuzhuan brick tea improves the beneficial function on inhibiting fat deposition显示文摘 | Yuxuan Peng Zhe Xiong Juan Li Jian-an Huang Cuiqin Teng Yushun Gong Zhonghua Liu | 2014 | International Journal of Food Sciences and Nutrition2014，，5： | 1 |
| 7 | Genetic Modification and Screening in Rat Using Haploid Embryonic Stem Cells显示文摘 | Wei Li Xin Li Tianda Li Ming-Gui Jiang Haifeng Wan Guan-Zheng Luo Chunjing Feng Xiaolong Cui Fei Teng Yan Yuan Quan Zhou Qi Gu Ling Shuai Jiahao Sha Yamei Xiao Liu Wang Zhonghua Liu Xiu-Jie Wang Xiao-Yang Zhao Qi Zhou | 2013 | Cell Stem Cell2013，，： | 1 |
| 8 | Correlation Analysis of Quantitative Microscopic Characteristics and Polysaccharide Content of Dendrobium officinale from Guangxi显示文摘[Objectives]The paper was to investigate the correlation between the microscopic characteristic constants and the index component polysaccharide content of Dendrobium officinale from Guangxi.[Methods]The paraffin sections of D.officinale were made,and the microscopic characteristic constants of D.officinale stem,including cross section area,vascular bundle area and phloem area,were determined by NIS-Elements D microscopic image processing software.The contents of polysaccharides in D.officinale were determined by UV-Vis spectrophotometry,and the quantitative values of microscopic characteristics of D.officinale with different growth years were analyzed by SPSS 23.0(IBM SPSS Statistics 23.0)data statistical software.The correlation between quantitative microscopic characteristics of D.officinale and its polysaccharide content was analyzed.[Results]According to the correlation between the index component of D.officinale and its quantitative microscopic characteristics,the measurement indexes,the percentage of vascular bundle in the cross section area and the percentage of hard structure in the cross section area,were strongly negatively correlated with polysaccharide content.[Conclusions]The microscopic characteristic constant values of vascular bundles of D.officinale can be used as a method to verify polysaccharide content. | Liuyuan FAN Hua ZHU Jianbei TENG Zhonghua DAI Piaoling HUANG | 2022 | Medicinal Plant2022，13，4： | 0 |
| 9 | Research Situation of Polysaccharides of Dendrobii Officinalis Caulis显示文摘This study was conducted to investigate the changes of polysaccharides content in Dendrobii Officinalis Caulis and its pharmacological action. The results showed that different growth years,different periods,different habitats and different external factors had influences on the change of polysaccharide content in Dendrobii Officinalis Caulis. Dendrobii Officinalis Caulis has certain effects of enhancing immunity,resisting fatigue and oxidation,promoting digestion and salivation secretion,reducing blood sugar and blood pressure,resisting tumor and liver injury,and relaxing bowels. Dendrobii Officinalis Caulis polysaccharides are the main effective components in Dendrobii Officinalis Caulis. The control of Dendrobii Officinalis Caulis polysaccharide content is of great significance to the assurance of the yield and quality of Dendrobii Officinalis Caulis material. | Hua ZHU Liuyuan FAN Zhonghua DAI Jianbei TENG Fangfang DA Peng LI Pingfeng LI | 2017 | Agricultural Biotechnology2017，6，6： | 0 |
| 10 | Overexpression of Stella improves the efficiency of nuclear transfer reprogramming显示文摘In mammalians,the state of a somatic cell can be reversed from the terminal state to the totipotent state by means of somatic cell nuclear transfer(SCNT)(Gurdon,1962)or induced pluripotent stem cells(iPSCs)(Takahashi and Yamanaka,2006).The DNA methylation and transcriptome profiles of embryonic stem cells(ESCs)derived from SCNT embryos(NT-ESCs)correspond | Leyun Wang Fei Teng Xuewei Yuan Chao Liu qiang Wang Yufei Li Tongtong Cui Tianda Li Zhonghua Liu Qi Zhou | 2017 | Journal of Genetics and Genomics2017，44，7： | 0 |
| 11 | Genetic mapping and identification of Lg^(f) loci controlling green fuzz in Upland cotton(Gossypium hirsutum L.)显示文摘Naturally colored cotton fiber is environment-friendly but has monotonous color and poor fiber quality.Identification of green fiber or fuzz genes would aid in investigating the biosynthesis of green pigments in cotton fibers. In this study, we established a mapping population and found that the Lg^(f) trait(white lint and green fuzz) from Gossypium hirsutum race latifolium is controlled by an incompletely dominant gene.The Lg^(f) locus was mapped to a 71-kb interval on chromosome 21 containing seven genes, including a transcription factor with similarity to Arabidopsis MYB9. Harboring 13 SNPs and a 4-bp insertion/deletion in its promoter, GhMYB9 was highly up-regulated in the critical period for green pigment development in fuzz. Virus-induced gene silencing of GhMYB9 in a green-fuzz accession of G. hirsutum race latifolium TX-41 conferred white or light green fuzz. These results suggest that GhMYB9 is an important contributor to green pigments in cotton fiber and shed light on the regulatory mechanism controlling green pigmentation. | Dexin Liu Xueying Liu Yao Su Xiao Zhang Kai Guo Zhonghua Teng Jian Zhang Dajun Liu Zhengsheng Zhang | 2021 | The Crop Journal2021，9，4： | 0 |
| 12 | Pharmacognosy Identification of Xiaohuangcao (Dendrobium loddigesi Rolfe)显示文摘[Objectives]Pharmacognosy identification was performed on Xiaohuangcao ( Dendrobium loddigesi Rolfe).[Methods]The medicinal materials were identified through original plants,characters,microscopic structure and thin-layer identification characteristics.[Results]D.loddigesi has obvious plant morphology,characters,microscopic structure and thin-layer identification characteristics.The stem of Xiaohuangcao is slender and cylindrical,and the surface is golden yellow;and the fiber bundles outside the vascular bundles are crescent-shaped or semi-circular in the stem transection.For the powder,crystal fiber can be observed;the vascular bundles are embedded with siliceous block cells;and there are more starch grains.In the thin layer chromatography,petroleum ether-ethyl acetate-butanone-glacial acetic acid (8.5∶ 3.5∶ 1.5∶ 5 d) was used as a developing solvent,and 10% sulfuric acid ethanol solution was used as a color developing agent.[Conclusions]The research results provide reference for the application of the medicinal material and the formulation of its related quality standards. | Miao ZHANG Li LI Jianbei TENG Hua ZHU Zhonghua DAI Long CHEN | 2019 | Agricultural Biotechnology2019，8，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员