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| 1 | Expression of ipt gene driven by tomato fruit specific promoter and its effects on fruit development of tomato显示文摘Fruit specific promoter (2A12) from Lycopersi-com esculentum and cDNA of isopentenyl-transferase (ipt) from Ti plasmid of Agrobacterium tumerfaciens C58 were cloned by PCR procedure respectively. Two plant expression vectors with 2A12/gus or 2A12/ipt were respectively constructed. These two chimeric genes were transferred into tomato by Agrobacterium mediated procedure. The results of Southern hybridization showed that the fusion genes had been integrated into tomatoes. The result of gus histochemi-cal staining showed that 2A12 had high fruit specific expressive capability in transgenic tomato. The ipt expression resulted in accumulation of high level of cytokinins (CTKs) in fruit lead to developmental changes in fruits and seeds. The fruit of ipt transformed tomato had the hyperplastic placenta with very few seeds or even seedless. The shelf life of transgenic fruits elongated for 1-2 weeks. The ratio of fruit set, the dry weight of fruit and the crude protein content in fruit were increased, while | Zichao Mao Qiuju Yu Wei Zhen Junyi Guo Yuanlei Hu Yin Gao Zhongping Lin | 2002 | Chinese Science Bulletin2002，47，11： | 6 |
| 2 | Cloning and expression pattern of a dehydrin-like BDNI gene from drought-tolerant Boea crassifolia Hemsl.显示文摘A 500-bp cDNA fragment was amplified via RT-PCR from drought-induced total RNA of the drought-tolerant B. crassifolia Hemsl. using primers based on the sequence of published dehydrin conserved region. By using 5’ RACE, full-length coding region (1 148 bp) of BDN1 gene was produced. It is a new member of the dehydrin gene family. Southern analysis indicated that BDN1 is present in the 6. crassifolia genome as a single-copy gene. Northern analysis revealed that its expression is inducible by drought and cold stresses as well as ABA application. | ZHAO Huiwu, LIU Han, YU Haiyuan, HU Yuanlei, GAO Yin, LI Zhenyu & LIN ZhongpingNational Laboratory of Protein Engineering and Plant Genetic Engineering , Peking University, Beijing 100871, China Botanical Institute, Chinese Academy of Sciences, Beijing 100093, China Correspondence should be addressed to Lin Zhongping | 2000 | Chinese Science Bulletin2000，45，22： | 3 |
| 3 | Overview on acute-on-chronic liver failure显示文摘肝失败(LF ) 在各种各样的病原学导致的肝的合成, detoxification,和新陈代谢被定义为严重机能障碍。LF 的临床的表示典型地包括严重黄疸,凝结混乱,肝的 encephalopathy,和腹水。LF 能被分类进尖锐 LF, acute-on-chronic LF (ACLF ) ,和长期的 LF。ACLF 与唯一的临床的表示和结果作为不同症候群被表明了。ACLF 的严厉,治愈可能性,和可逆性吸引了可观的注意。在 ACLF 相关的概念,诊断标准,致病,和治疗的显著开发被完成了。然而,这疾病,特别它的诊断标准,仍然保持争论。在这份报纸,我们全身地由彻底地比较在东方和西方之间的重要调查结果从它的定义,病原学, pathophysiology,病理,和临床的表示考察了 ACLF 的当前的理解到管理从另外的区域的国家,以及那些。我们也讨论了争吵,挑战,和对为 ACLF 支持诊断和治疗的标准化和优化的未来研究的需要。 | Jing Zhang Shan Gao Zhongping Duan Ke-Qin Hu | 2016 | Frontiers of Medicine2016，10，1： | 3 |
| 4 | Enhanced late blight resistance of transgenic potato expressing glucose oxidase under the control of pathogen-inducible promoter显示文摘To engineer crop disease resistance by utilizing natural defense mechanism that was expressed in the incompatible host-pathogen interactions is expected to result in a durable and broad-spectrum resistance. In order to prove this viewpoint, we amplified the coding region of the glucose oxidase (GO) gene from Aspergillus nigervia PCR and fused it to the pathogen-inducible promoter, Prp1-1. The chimeric gene was cloned into a plant expression vector and conjugated into Agrobacterium. Twenty-three transgenic potato plants were obtained by Agrobacterium-mediated transformation. The integration of GO gene was confirmed by Southern hybridization and the GO gene expression was identified with Kl-starch color reaction. Phytophthora infestans inoculation revealed that the expression of the chimeric transgene was induced by pathogen infection. Most of the transgenic plants exhibited various degrees of enhanced disease resistance. Four of them had lesion sizes reduced to less than half of the non-transgenic | Wei Zhen Xi Chen Haobo Liang Yuanlei Hu Yin Gao Zhongping Lin | 2000 | Chinese Science Bulletin2000，45，21： | 2 |
| 5 | An Effective Digital Watermarking Algorithm for Binary Text Image显示文摘Aiming at the binary text image’s characteristics of simple pixel, complex texture and bad immunity of information concealment, a digital watermarking embedment location choosing method has been put forward based upon compatible roughness set. The method divides binary text image into different equivalent classes. Equivalent classes are further divided into different subclasses according to each pixel’s degree and texture changes between blocks. Through properties’ combination, the embedment block and location which are fit for watermarking are found out. At last, different binary text images are chosen for emulation experiment. After being embedded, the image is compressed in JPIG-2. Gaussian noise, salt & pepper noise are added and cutting is employed to imitate the actual environment in which images may suffer from various attacks and interferences. The result shows that the detector has a sound testing effect under various conditions. | HU Zhihua QIN Zhongping | 2006 | Wuhan University Journal of Natural Sciences2006，11，6： | 2 |
| 6 | Expression profile of a PAL gene from Astragalus membranaceus var. Mongholicus and its crucial role in flux into flavonoid biosynthesis显示文摘 | Rongrong Liu Shaohua Xu Jialin Li Yuanlei Hu Zhongping Lin | 2006 | Plant Cell Reports2006，，7： | 1 |
| 7 | Wide-angle seismic imaging of the high velocity subbasalt显示文摘 | Hu Zhongping Wu Dali Guan Luping | 2003 | Expanded Abstracts of 73th Annual Internat SEG Mtg2003，11，421： | 1 |
| 8 | Inducible Excision of Selectable Marker Gene from Transgenic Plants by the Cre/lox Site-specific Recombination System显示文摘 | Yong Wang Bojun Chen Yuanlei Hu Jingfu Li Zhongping Lin | 2005 | Transgenic Research2005，，5： | 1 |
| 9 | Single-wavelength-excited fluorogenic nanoprobe for accurate realtime ratiometric analysis of broad pH fluctuations in mitophagy显示文摘Mitophagy has a critical role in maintaining cellular homeostasis through acidic lysosomes engulfing excess or impaired mitochondria,thereby pH fluctuation is one of the most significant indicators for tracking mitophagy.Then such precise pH tracking demands the fluorogenic probe that has tailored contemporaneous features,including mitochondrial-specificity,excellent biocompatibility,wide pH-sensitive range of 8.0–4.0,and especially quantitative ability.However,available molecular probes cannot simultaneously meet all the requirements since it is extremely difficult to integrate multiple functionalities into a single molecule.To fully address this issue,we herein integrate two fluorogenic pH sensitive units,a mitochondria-specific block,cellpenetrating facilitator,and biocompatible segments into an elegant silica nano scaffold,which greatly ensures the applicability for real-time tracking of pH fluctuations in mitophagy.Most significantly,at a single wavelength excitation,the integrated pHsensitive units have spectra-distinguishable fluorescence towards alkaline and acidic pH in a broad range that covers mitochondrial and lysosomal pH,thus enabling a ratiometric analysis of pH variations during the whole mitophagy.This work also provides constructive insights into the fabrication of advanced fluorescent nanoprobes for diverse biomedical applications. | Xin Zhang Juan Chen Jiwen Hu Anna du Rietz Xiongyu Wu Ruilong Zhang Zhongping Zhang Kajsa Uvdal Zhangjun Hu | 2022 | Nano Research2022，15，7： | 1 |
| 10 | Efficient transformation of the medicinal mushroomGanoderma lucidum显示文摘 | Li Sun Huaqing Cai Weihong Xu Yuanlei Hu Yin Gao Zhongping Lin | 2001 | Plant Molecular Biology Reporter2001，，： | 1 |
| 11 | A penalty function method for solving inverse optimal value prob- lem显示文摘 | Yibing Lv Tiesong Hu Zhongping Wan | 2008 | Journal of Computational and Applied Mathe- matics2008，220，1： | 1 |
| 12 | Evaluation of GOCI sensitivity for At-Sensor radiance and GDPS-Retrieved chlorophyll- a products显示文摘 | Chuanmin Hu Lian Feng ZhongPing Lee | 2012 | Ocean Science Journal2012，，3： | 1 |
| 13 | A phytocyanin-related early nodulin-like gene, BcBCP1 , cloned from Boea crassifolia enhances osmotic tolerance in transgenic tobacco显示文摘 | Hanying Wu Ye Shen Yuanlei Hu Sijun Tan Zhongping Lin | 2011 | Journal of Plant Physiology2011，，9： | 1 |
| 14 | New perspective on the natural course of chronic HBV infection显示文摘长期的肝炎 B 病毒(HBV ) 感染在社会上是对公共健康和庞大的负担的重要威胁。为长期的 HBV 感染负责的机制仍然保持糟糕理解。长期的 HBV 感染的自然功课的更好的理解可以在在设计新抗病毒的策略位于这疾病和帮助下面的机制上打开新灯。当通常是的无人干扰的进程由 HBV e 标记抗原(HBeAg ) seroconversion 或由与对 HBV 感染的假定主机回答联系的不同阶段描绘了,长期的 HBV 感染的自然路线通常被看。然而,任何一个都不这些描述捕获或加亮决定长期的 HBV 感染的自然功课的核心事件。在这评论,我们简短在这个题目上介绍当前的知识并且解释发生在感染期间的事件的意义和含意。核心前异种病毒长期地在鸭肝炎 B 在野类型的病毒的病毒的人口追随者消除变得占优势感染的动物。在的联合事件第一有病毒的清理,清除野类型的病毒然后有有变异的病毒的清除的肝是的野类型的病毒的再感染与长期的 HBV 感染在下面的自然功课的理解高度相关对待并且未经治疗的条件。在我们的新观点,长期的 HBV 感染的一堂一般自然功课包括病毒的清理和再感染的周期,并且这骑车延长长期的 HBV 感染功课。在长期的 HBV 感染的自然功课上考察出版数据能在起始的数据解释减少失踪的重要的点的可能性。 | Yong-Yuan Zhang Ke-Qin Hu Zhongping Duan | 2014 | Frontiers of Medicine2014，8，2： | 1 |
| 15 | Calculation of distribution of effective reflection area for space-borne laser retro-reflector array显示文摘The effective area of space-borne laser retro-reflector directly influences the intensity of the return signals for satellite laser ranging. The configuration of the retro-reflector array determines the effective area. The formula for the effective area of the space-borne retro-reflector array with different inclination angles at arbitrary celestial zone was deduced in this paper. As an example, the calculation result about effective area for the retro-reflector array of the Shenzhou-IV orbital module was given. | HU Jingfu LI Xin YANG Fumin CHEN Wanzhen ZHANG Zhongping | 2004 | Science China(Physics,Mechanics & Astronomy)2004，47，3： | 1 |
| 16 | Application of an optimization to satellite ocean color imageray:a case study in Southwest Florida coastal water显示文摘 | Zhongping Lee Frank E Muller-Karger | 2003 | Ocean Remote Sensing and Applications Proceedings of SPIE2003，4892，： | 1 |
| 17 | Wide-angle seismic imaging of the high-velocity sub-basalt 显示文摘 | Zhongping Hu Dali Wu Luping Guan | 2003 | Expanded Abstracts of 73rd Annual Internat SEG Mtg2003，11，421： | 1 |
| 18 | Satellite laser ranging experiment with sub-centimeter single-shot ranging precision at Shanghai Observatory显示文摘 | Fumin Yang Wanzhen Chen Zhongping Zhang Juping Chen Jingfu Hu Xin Li I. Prochazka K. Hamal | 2003 | Science in China Series G: Physics Mechanics and Astronomy2003，，1： | 1 |
| 19 | cAMP-PGC1α途径诱导的抗Warburg效应驱动胶质母细胞瘤细胞分化为星形胶质细胞显示文摘文章简介多形性胶质母细胞瘤(GBM)是人类最具侵袭性的肿瘤之一。虽然分化治疗已被提出作为治疗GBM的潜在方法,但诱导分化的机制仍然很不清楚。在此,课题组利用c AMP激活剂建立了GBM诱导分化模型,该模型特异性地指导GBM分化为星形胶质细胞。 | Fan Xing Yizhao Luan Jing Cai Sihan Wu Jialuo Mai Jiayu Gu Haipeng Zhang Kai Li Yuan Lin Xiao Xiao Jiankai Liang Yuan Li Wenli Chen Yaqian Tan Longxiang Sheng Bingzheng Lu Wanjun Lu Mingshi Gao Pengxin Qiu Xingwen Su Wei Yin Jun Hu Zhongping Chen Ke Sai Jing Wang Furong Chen Yinsheng Chen Shida Zhu Dongbing Liu Shiyuan Cheng 谢志 朱文博 颜光美 | 2018 | 科学新闻2018，0，4： | 1 |
| 20 | A penalty function method for solving weak pricecontrol problem显示文摘 | Lü Yibing Hu Tiesong Wan Zhongping | 2007 | Applied Mathematics and Computation2007，186，： | 1 |
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