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| 1 | 中药与天然药物2015~2020年研究亮点评述显示文摘中药与天然药物在2015~2020年取得多项突破性进展,屠呦呦青蒿素研究获得诺贝尔奖促使国内外掀起研究中药与天然药物的热潮,“甘露寡糖二酸”、“桑枝总生物碱片”等原创药物获得新药证书;多项研究成果入选年度“中国十大医学进展”,在Nature、Science、New England Journal of Medicine、Lancet等国际顶级期刊发表了高水平的研究论文,本文梳理总结了这五年期间国内外科学家在国际著名期刊发表中药与天然药物相关的亮点学术成果,并对其在化学、药物资源、药理、制剂、新药开发等相关领域取得的重要进展进行了评述,以期追踪和报道中药与天然药物领域发展的前沿和热点,并通过对其分析得出学科发展的启示和展望。 | 陈士林 孙奕 万会花 张晗 赵庆贺 | 2020 | 药学学报2020，55，12： | 24 |
| 2 | The Reference Genome Sequence of Scutellaria baicalensis Provides Insights into the Evolution of Wogonin Biosynthesis显示文摘Scutellaria baicalensis Georgi is important in Chinese traditional medicine where preparations of dried roots,'Huang Qin,' are used for liver and lung complaints and as complementary cancer treatments. We report a high-quality reference genome sequence for S. baicalensis where 93% of the 408.14-Mb genome has been assembled into nine pseudochromosomes with a super-N50 of 33.2 Mb. Comparison of this sequence with those of closely related species in the order Lamiales, sesamum indicum and Salvia splendens,revealed that a specialized metabolic pathway for the synthesis of 4'-deoxyflavone bioactives evolved in the genus Scu-tellaria. We found that the gene encoding a specific cinnamate coenzyme A ligase likely obtained its newfunc- tion following recent mutations, and that four genes encoding enzymes in the 4'-deoxyflavone pathway are present as tandem repeats in the genome of S. baicalensis. Further analyses revealed that gene duplications, segmental duplication, gene amplification, and point mutations coupled to gene neo- and subfunctionaliza-tions were involved in the evolution of 4'-deoxyflavone synthesis in the genus Scutellaria. Our study not only provides significant insight into the evolution of specific flavone biosynthetic pathways in the mint family, Lamiaceae, but also will facilitate the development of tools for enhancing bioactive productivity by metabolic engineering in microbes or by molecular breeding in plants. The reference genome of S. baicalensis is also useful for improving the genome assemblies for other members of the mint family and offers an important foundation for decoding the synthetic pathways of bioactive compounds in medicinal plants. | Qing Zhao Jun Yang Meng-Ying Cui Jie Liu Yumin Fang Mengxiao Yan Wenqing Qiu Huiwen Shang Zhicheng Xu Reheman Yidiresi Jing-Ke Weng Tomas Pluskal Marielle Vigouroux Burkhard Steuernagel Yukun Wei Lei Yang Yonghong Hu Xiao-Ya Chen Cathie Martin | 2019 | Molecular Plant2019，12，7： | 23 |
| 3 | 博落回化学成分及其药理活性研究进展显示文摘博落回是罂粟科博落回属多年生直立草本植物,味苦,辛,性寒,温,大毒,广泛分布于我国长江以南、南岭以北的大部分省区,文献记载其具有悠久的药用历史。文献记载其具有祛风镇痛、解毒消肿等功用,主要治疗毒脓肿、恶疮溃疡、疥癣、滴虫性阴道炎等;亦有杀虫、止痒之功效。博落回的主要化学成分为异喹啉生物碱,其中血根碱、白屈菜红碱、原阿片碱和别隐品碱含量较高。此外,还含有苯丙素类、甾体类、有机酸类、酚类和挥发油等。博落回的药理作用主要为具抗菌、抗炎、抗肿瘤和改善肝功能等。在农业上可作为植物源杀虫剂和抑菌剂,也可当作饲料添加剂用于畜牧业。近年来,有大量关于博落回的文献报道,尤其是单体化合物的分离鉴定和活性研究。该文通过查阅国内外文献,对博落回的化学成分及其药理作用进行了系统总结,为博落回提取物及其单体化合物的开发利用提供理论依据。 | 陈伟 张锐 雷思敏 左怀龙 陈施吉 常耀东 | 2020 | 中国实验方剂学杂志2020，26，3： | 19 |
| 4 | Herbgenomics: A stepping stone for research into herbal medicine显示文摘From the prehistoric era until the publishing of the Compendium of Materia Medica and the first scientific Nobel Prize in the Chinese mainland for Tu's discovery on anti-malarial tablets,each milestone and stepping stone in the developmental history of herbal medicine involved intrepid exploration,bold hypothesis formulation,and cautious verification.After thousands of years of discovery and development,herbal research has entered a new era—the era of herbgenomics.Herbgenomics combines herbal and genomic research,bridging the gap between traditional herbal medicine and cutting-edge omics studies.Therefore,it provides a general picture of the genetic background of traditional herbs,enabling researchers to investigate the mechanisms underlying the prevention and treatment of human diseases from an omics perspective. | Haoyu Hu Xiaofeng Shen Baosheng Liao Lu Luo Jiang Xu Shilin Chen | 2019 | Science China(Life Sciences)2019，62，7： | 13 |
| 5 | Trends in herbgenomics显示文摘From Shen Nong's Herbal Classic(Shennong Bencao Jing) to the Compendium of Materia Medica(Bencao Gangmu) and the first scientific Nobel Prize for the mainland of China, each milestone in the historical process of the development of traditional Chinese medicine(TCM) involves screening, testing and integrating. After thousands of years of inheritance and development,herbgenomics(bencaogenomics) has bridged the gap between TCM and international advanced omics studies, promoting the application of frontier technologies in TCM. It is a discipline that uncovers the genetic information and regulatory networks of herbs to clarify their molecular mechanism in the prevention and treatment of human diseases. The main theoretical system includes genomics, functional genomics, proteomics, transcriptomics, metabolomics, epigenomics, metagenomics, synthetic biology, pharmacogenomics of TCM, and bioinformatics, among other fields. Herbgenomics is mainly applicable to the study of medicinal model plants, genomic-assisted breeding, herbal synthetic biology, protection and utilization of gene resources, TCM quality evaluation and control, and TCM drug development. Such studies will accelerate the application of cutting-edge technologies, revitalize herbal research, and strongly promote the development and modernization of TCM. | Tianyi Xin Yu Zhang Xiangdong Pu Ranran Gao Zhichao Xu Jingyuan Song | 2019 | Science China(Life Sciences)2019，62，3： | 10 |
| 6 | 博落回资源为示范的中药材全产业链研究与综合利用显示文摘博落回为新发掘的农用产品之重要中药资源,是异喹啉类生物碱尤其是血根碱重要的来源植物,在欧洲已作为饲用抗生素重要的替代产品来使用(如Sangrovit),本文介绍湖南农业大学'兽用中药资源与中兽药创制'国家农业科研杰出人才研究团队从资源生物学、野生变家种、资源化学、品质评价与代谢分析、综合利用开发等方面进行的博落回全产业链研究。 | 曾建国 | 2017 | 中国现代中药2017，19，10： | 9 |
| 7 | Abnormal expression of bHLH3 disrupts a flavonoid homeostasis network,causing differences in pigment composition among mulberry fruits显示文摘Mulberry fruits with high concentrations of anthocyanins are favored by consumers because of their good taste,bright color,and high nutritional value.However,neither the regulatory mechanism controlling flavonoid biosynthesis in mulberry nor the molecular basis of different mulberry fruit colors is fully understood.Here,we report that a flavonoid homeostasis network comprising activation and feedback regulation mechanisms determines mulberry fruit color.In vitro and in vivo assays showed that MYBA-bHLH3-TTG1 regulates the biosynthesis of anthocyanins,while TT2L1 and TT2L2 work with bHLH3 or GL3 and form a MYB-bHLH-WD40(MBW)complex with TTG1 to regulate proanthocyanidin(PA)synthesis.Functional and expression analyses showed that bHLH3 is a key regulator of the regulatory network controlling mulberry fruit coloration and that MYB4 is activated by MBW complexes and participates in negative feedback control of the regulatory network to balance the accumulation of anthocyanins and proanthocyanidins.Our research demonstrates that the interaction between bHLH3 and MYB4 in the homeostasis regulatory network ensures that the fruits accumulate desirable flavonoids and that this network is stable in pigmentrich mulberry fruits.However,the abnormal expression of bHLH3 disrupts the balance of the network and redirects flavonoid metabolic flux in pale-colored fruits,resulting in differences in the levels and proportions of anthocyanins,flavones,and flavonols among differently colored mulberry fruits(red,yellow,and white).The results of our study reveal the molecular basis of the diversity of mulberry fruit colors. | Han Li Zhen Yang Qiwei Zeng Shibo Wang Yiwei Luo Yan Huang Youchao Xin Ningjia He | 2020 | Horticulture Research2020，7，1： | 8 |
| 8 | 葫芦素B通过抑制Hippo-YAP通路抑制结肠癌细胞转移的机制研究显示文摘目的:本文旨在探讨葫芦素B (cucurbitacin B,CuB)通过作用于Hippo-YAP信号通路抑制结肠癌(colorectal carcinoma,CRC)细胞增殖、侵袭和迁移的作用及具体分子机制。方法:通过MTT法及克隆形成实验检测CuB对细胞增殖能力的影响;通过Transwell以及划痕实验检测CuB对细胞的侵袭和迁移能力的影响;通过Western blot检测CuB处理后转移相关蛋白及Hippo-YAP信号通路相关蛋白的表达变化。结果:CuB可抑制CRC细胞的增殖和转移及促进细胞凋亡;CuB是通过抑制YAP及其下游靶分子Cyr61和c-Myc,上调CRC细胞中LATS1的表达来发挥作用的。结论:CuB可以抑制结肠癌细胞生长及转移且CuB可能通过抑制Hippo-YAP信号通路来发挥作用。CuB可能作为新型的YAP抑制剂用于结肠癌的治疗。 | 周彤 杨书胜 罗明洲 向可 刘莹 陈森 | 2020 | 湖北医药学院学报2020，39，1： | 6 |
| 9 | Insights into triterpene synthesis and unsaturated fatty-acid accumulation provided by chromosomallevel genome analysis of Akebia trifoliata subsp . australis显示文摘Akebia trifoliata subsp.australis is a well-known medicinal and potential woody oil plant in China.The limited genetic information available for A.trifoliata subsp.australis has hindered its exploitation.Here,a high-quality chromosomelevel genome sequence of A.trifoliata subsp.australis is reported.The de novo genome assembly of 682.14 Mb was generated with a scaffold N50 of 43.11 Mb.The genome includes 25,598 protein-coding genes,and 71.18%(485.55 Mb)of the assembled sequences were identi fi ed as repetitive sequences.An ongoing massive burst of long terminal repeat(LTR)insertions,which occurred~1.0 million years ago,has contributed a large proportion of LTRs in the genome of A.trifoliata subsp.australis.Phylogenetic analysis shows that A.trifoliata subsp.australis is closely related to Aquilegia coerulea and forms a clade with Papaver somniferum and Nelumbo nucifera,which supports the well-established hypothesis of a close relationship between basal eudicot species.The expansion of UDP-glucoronosyl and UDP-glucosyl transferase gene families and fi-amyrin synthase-like genes and the exclusive contraction of terpene synthase gene families may be responsible for the abundant oleanane-type triterpenoids in A.trifoliata subsp.australis.Furthermore,the acyl-ACP desaturase gene family,including 12 stearoyl-acyl-carrier protein desaturase(SAD)genes,has expanded exclusively.A combined transcriptome and fatty-acid analysis of seeds at fi ve developmental stages revealed that homologs of SADs,acyl-lipid desaturase omega fatty acid desaturases(FADs),and oleosins were highly expressed,consistent with the rapid increase in the content of fatty acids,especially unsaturated fatty acids.The genomic sequences of A.trifoliata subsp.australis will be a valuable resource for comparative genomic analyses and molecular breeding. | Hui Huang Juan Liang Qi Tan Linfeng Ou Xiaolin Li Caihong Zhong Huilin Huang Ian Max Moller Xianjin Wu Songquan Song | 2021 | Horticulture Research2021，8，1： | 5 |
| 10 | 药用植物基因组测序及功能基因组学研究进展显示文摘由于野生资源缺乏,药用植物主要通过人工种植培育获得.而高通量测序的到来大大加快了该领域研究的进展.通过组学技术研究药用植物的遗传信息及其调控网络,能够完善药用植物繁殖和栽培的技术体系,并更好地阐明药用植物预防、治疗人类疾病的分子机制.综述了药用植物基因组测序以及功能基因挖掘等研究进展,内容涉及基因组结构、非生物胁迫、活性成分、生长调控等方面,最后对当前药用植物分子生物学研究进行了讨论.旨在为设计更完备的药用植物基因组测序实验提供参考. | 李杰 蔡嘉慧 王慧中 孟一君 | 2021 | 杭州师范大学学报(自然科学版)2021，20，4： | 4 |
| 11 | A high-quality genome assembly of Morinda officinalis,a famous native southern herb in the Lingnan region of southern China显示文摘Morinda officinalis is a well-known medicinal and edible plant that is widely cultivated in the Lingnan region of southern China.Its dried roots(called bajitian in traditional Chinese medicine)are broadly used to treat various diseases,such as impotence and rheumatism.Here,we report a high-quality chromosome-scale genome assembly of M.officinalis using Nanopore single-molecule sequencing and Hi-C technology.The assembled genome size was 484.85Mb with a scaffold N50 of 40.97 Mb,and 90.77%of the assembled sequences were anchored on eleven pseudochromosomes.The genome includes 27,698 protein-coding genes,and most of the assemblies are repetitive sequences.Genome evolution analysis revealed that M.officinalis underwent core eudicotγgenome triplication events but no recent whole-genome duplication(WGD).Likewise,comparative genomic analysis showed no large-scale structural variation after species divergence between M.officinalis and Coffea canephora.Moreover,gene family analysis indicated that gene families associated with plant–pathogen interactions and sugar metabolism were significantly expanded in M.officinalis.Furthermore,we identified many candidate genes involved in the biosynthesis of major active components such as anthraquinones,iridoids and polysaccharides.In addition,we also found that the DHQS,GGPPS,TPS-Clin,TPS04,sacA,and UGDH gene families—which include the critical genes for active component biosynthesis—were expanded in M.officinalis.This study provides a valuable resource for understanding M.officinalis genome evolution and active component biosynthesis.This work will facilitate genetic improvement and molecular breeding of this commercially important plant. | Jihua Wang Shiqiang Xu Yu Mei Shike Cai Yan Gu Minyang Sun Zhan Liang Yong Xiao Muqing Zhang Shaohai Yang | 2021 | Horticulture Research2021，8，1： | 4 |
| 12 | Genome and transcriptome of Papaver somniferum Chinese landrace CHM indicates that massive genome expansion contributes to high benzylisoquinoline alkaloid biosynthesis显示文摘Opium poppy(Papaver somniferum)is a source of morphine,codeine,and semisynthetic derivatives,including oxycodone and naltrexone.Here,we report the de novo assembly and genomic analysis of P.somniferum traditional landrace‘Chinese Herbal Medicine’.Variations between the 2.62 Gb CHM genome and that of the previously sequenced high noscapine 1(HN1)variety were also explored.Among 79,668 protein-coding genes,we functionally annotated 88.9%,compared to 68.8%reported in the HN1 genome.Gene family and 4DTv comparative analyses with three other Papaveraceae species revealed that opium poppy underwent two whole-genome duplication(WGD)events.The first of these,in ancestral Ranunculales,expanded gene families related to characteristic secondary metabolite production and disease resistance.The more recent species-specific WGD mediated by transposable elements resulted in massive genome expansion.Genes carrying structural variations and large-effect variants associated with agronomically different phenotypes between CHM and HN1 that were identified through our transcriptomic comparison of multiple organs and developmental stages can enable the development of new varieties.These genomic and transcriptomic analyses will provide a valuable resource that informs future basic and agricultural studies of the opium poppy. | Li Pei Baishi Wang Jian Ye Xiaodi Hu Lihong Fu Kui Li Zhiyu Ni Zhenlong Wang Yujie Wei Luye Shi Ying Zhang Xue Bai Mengwan Jiang Shuhui Wang Chunling Ma Shujin Li Kaihui Liu Wanshui Li Bin Cong | 2021 | Horticulture Research2021，8，1： | 4 |
| 13 | Over 100 Million Years of Enzyme Evolution Underpinning the Production of Morphine in the Papaveraceae Family of Flowering Plants显示文摘Phylogenomic analysis of whole genome sequences of five benzylisoquinoline alkaloid(BIA)-producing species from the Ranunculales and Proteales orders of flowering plants revealed the sequence and timing of evolutionary events leading to the diversification of these compounds.(S)-Reticuline is a pivotal intermediate in the synthesis of many BIAs and our analyses revealed parallel evolution between the two orders,which diverged122 million years ago(MYA).Berberine is present in species across the entire Ranunculales,and we found co-evolution of genes essential for production of the protoberberine class.The benzophenanthridine class,which includes the antimicrobial compound sanguinarine,is specific to the Papaveraceae family of Ranunculales,and biosynthetic genes emerged after the split with the Ranunculaceae family110 MYA but before the split of the three Papaveraceae species used in this study at77 MYA.The phthalideisoquinoline noscapine and morphinan class of BIAs are exclusive to the opium poppy lineage.Ks estimation of paralogous pairs indicates that morphine biosynthesis evolved more recently than 18 MYA in the Papaver genus.In the preceding 100 million years gene duplication,neofunctionalization and recruitment of additional enzyme classes,combined with gene clustering,gene fusion,and gene amplification,resulted in emergence of medicinally valuable BIAs including morphine and noscapine. | Yi Li Thilo Winzer Zhesi He Ian AGraham | 2020 | Plant Communications2020，1，2： | 3 |
| 14 | The chromosome-level reference genome of Coptis chinensis provides insights into genomic evolution and berberine biosynthesis显示文摘Coptis chinensis Franch, a perennial herb, is mainly distributed in southeastern China. The rhizome of C. chinensis hasbeen used as a traditional medicine for more than 2000 years in China and many other Asian countries. Thepharmacological activities of C. chinensis have been validated by research. Here, we present a de novo high-qualitygenome of C. chinensis with a chromosome-level genome of ~958.20 Mb, a contig N50 of 1.58 Mb, and a scaffold N50of 4.53 Mb. We found that the relatively large genome size of C. chinensis was caused by the amplification of longterminal repeat (LTR) retrotransposons. In addition, a whole-genome duplication event in ancestral Ranunculales wasdiscovered. Comparative genomic analysis revealed that the tyrosine decarboxylase (TYDC) and (S)-norcoclaurinesynthase (NCS) genes were expanded and that the aspartate aminotransferase gene (ASP5) was positively selected inthe berberine metabolic pathway. Expression level and HPLC analyses showed that the berberine content was highestin the roots of C. chinensis in the third and fourth years. The chromosome-level reference genome of C. chinensisprovides important genomic data for molecular-assisted breeding and active ingredient biosynthesis. | Da-xia Chen Yuan Pan Yu Wang Yan-Ze Cui Ying-Jun Zhang Rang-yu Mo Xiao-li Wu Jun Tan Jian Zhang Lian-an Guo Xiao Zhao Wenkai Jiang Tian-lin Sun Xiao-Di Hu Long-yun Li | 2021 | Horticulture Research2021，8，1： | 2 |
| 15 | 毛状根研究进展及其在博落回资源创新中的应用前景显示文摘发根农杆菌作为一种天然菌株可用于诱导多种植物产生毛状根性状。毛状根组织能够稳定高效地生产次生代谢产物,该研究领域正受到越来越多的关注。本文对发根农杆菌及毛状根的特性、遗传机制和应用进行了综述。同时结合本课题组前期研究基础,对毛状根在博落回资源创新与新药源途径开发上的应用前景进行展望。 | 夏丽琼 黄鹏 唐昭山 曾建国 | 2017 | 中国现代中药2017，19，10： | 2 |
| 16 | 基于B环构建的氯化两面针碱及其衍生物的合成研究进展显示文摘两面针为广西道地药材,近年来,广西野生两面针分布面积正逐年减小,资源短缺明显,且由于其水溶性差,导致从植物中提取分离的收率低。两面针含多种苯并[c]菲啶类生物碱,其主要活性成分氯化两面针碱抗肿瘤活性显著,开发前景广阔,科学家们一直致力于氯化两面针碱及其衍生物的全合成研究。其中苯并[c]菲啶骨架B环的构建是最有效的方法。将就其基于骨架B环构建的全合成方法及其衍生物的研究进行综述。 | 黄嘉咏 袁彩英 霍丽妮 陈睿 卢澄生 | 2018 | 广州化工2018，46，23： | 0 |
| 17 | 莲NnOMT和NnNMT基因家族的鉴定与分析显示文摘目的:莲苄基异喹啉生物碱(BIAs)的生物合成途径的解析具有重要的理论和经济价值,为了更好地促进莲BIAs生物合成途径的解析,本研究对莲氧甲基转移酶(NnOMT)、莲氮甲基转移酶(NnNMT)基因家族进行鉴定和生物信息学分析。方法:在莲全基因组的基础上,利用UniPort、美国国家生物技术信息中心(NCBI)数据库对莲NnOMT、NnNMT基因家族进行鉴定,分析其理化性质及亚细胞定位。利用TBtools、MEME、MEGA 11.0、FigTree 1.4.4等工具对前期鉴定出的NnOMT、NnNMT基因的系统发育、序列特征、基因结构、功能注释、顺式作用元件等进行分析。结果:共鉴定出61个莲NnOMT、NnNMT基因,编码的氨基酸数目介于168~580 aa,等电点范围为4.76~9.16,相对分子质量为18699.52~64934.53 Da,大部分表现为酸性且多为亲水蛋白,含有10个保守基序;京都基因与基因组百科全书(KEGG)分析共富集到12个通路,包括新陈代谢、异喹啉生物碱生物合成、苯丙烷的生物合成等;基因本体(GO)富集结果可视化显示,61个莲NnOMT、NnNMT基因共注释到32个类别,其中包括16项分子功能,如谷氨酸合成酶还原型烟酰胺腺嘌呤二核苷酸(NADH)活性、外肽酶活性,以及16项生物过程,如四氯化碳代谢过程、厌氧四氯化碳代谢过程、对外源生物刺激的反应。在莲NnOMT、NnNMT基因的启动子区存在多种顺式作用元件,主要为启动子和增强子区响应元件、光响应和茉莉酸甲酯响应元件。结论:该研究基于莲的基因组数据对61个莲NnOMT、NnNMT基因进行了全面的生物信息学分析,为莲NnOMT、NnNMT基因家族的基因结构和功能研究奠定了基础,并为莲BIAs的生物合成途径解析提供了参考。 | 陈竹荫 俞越童 郝晨阳 刘鸣茹 陈莎 | 2024 | 中国实验方剂学杂志2024，30，3： | 0 |
| 18 | 博落回叶提取物对小鼠溃疡性结肠炎的影响显示文摘【目的】研究博落回叶提取物(Macleaya cordata leaf extract,MCLE)对葡聚糖硫酸钠(DSS)诱导的溃疡性结肠炎(UC)小鼠的保护作用,为博落回叶的资源利用和药物开发提供理论依据。【方法】选择60只C57BL/6小鼠,随机分成6组,即对照组(CON)组、DSS模型组、美沙拉嗪(MES)阳性对照组(200 mg/kg)及MCLE高(MCLE-H,450 mg/kg)、中(MCLE-M,150 mg/kg)、低(MCLE-L,50 mg/kg)剂量组,试验期14 d。通过小鼠的一般症状、体重变化、疾病活动指数(DAI)、脾脏指数、结肠长度及组织病理学等指标评价MCLE对DSS诱导的小鼠结肠炎模型的治疗效果。使用ELISA和实时荧光定量PCR分别检测血清和结肠组织中白细胞介素-1β(IL-1β)、IL-6、肿瘤坏死因子α(TNF-α)的表达水平;比色法测定结肠组织中髓过氧化物酶(MPO)和一氧化氮(NO)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量;通过分析16S rRNA基因序列确定MCLE对肠道菌群的影响;采用气相色谱法测定结肠内容物中短链脂肪酸(SCFAs)的含量。【结果】MCLE处理组均极显著抑制了UC小鼠的体重降低和结肠长度的缩短(P<0.01),并显著降低了小鼠DAI、脾脏指数及结肠组织病理学评分(P<0.05);MCLE显著下调了结肠和血清中IL-1β、IL-6和TNF-α的表达(P<0.05),降低了结肠组织中MPO、NO和MDA的含量(P<0.05),增加了GSH-Px、SOD活性及CAT含量(P<0.05);与DSS组相比,拟杆菌属、另枝菌属、阿克曼氏菌属等有益菌属在MCLE给药组中丰度占比增加,病原菌如脱硫弧菌属等占比减少。此外,MCLE治疗还显著提高了UC小鼠肠内容物中SCFAs(乙酸、丙酸、丁酸)的水平(P<0.05)。【结论】MCLE可能是通过调节炎症因子水平、减轻氧化应激影响、维持肠道菌群稳态和恢复短链脂肪酸的产生发挥抗结肠炎作用。 | 马晓兰 董朕 岳美杉 常冠宇 曾建国 | 2023 | 中国畜牧兽医2023，50，11： | 0 |
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