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| 1 | The Reference Genome Sequence of Scutellaria baicalensis Provides Insights into the Evolution of Wogonin Biosynthesis显示文摘Scutellaria baicalensis Georgi is important in Chinese traditional medicine where preparations of dried roots,'Huang Qin,' are used for liver and lung complaints and as complementary cancer treatments. We report a high-quality reference genome sequence for S. baicalensis where 93% of the 408.14-Mb genome has been assembled into nine pseudochromosomes with a super-N50 of 33.2 Mb. Comparison of this sequence with those of closely related species in the order Lamiales, sesamum indicum and Salvia splendens,revealed that a specialized metabolic pathway for the synthesis of 4'-deoxyflavone bioactives evolved in the genus Scu-tellaria. We found that the gene encoding a specific cinnamate coenzyme A ligase likely obtained its newfunc- tion following recent mutations, and that four genes encoding enzymes in the 4'-deoxyflavone pathway are present as tandem repeats in the genome of S. baicalensis. Further analyses revealed that gene duplications, segmental duplication, gene amplification, and point mutations coupled to gene neo- and subfunctionaliza-tions were involved in the evolution of 4'-deoxyflavone synthesis in the genus Scutellaria. Our study not only provides significant insight into the evolution of specific flavone biosynthetic pathways in the mint family, Lamiaceae, but also will facilitate the development of tools for enhancing bioactive productivity by metabolic engineering in microbes or by molecular breeding in plants. The reference genome of S. baicalensis is also useful for improving the genome assemblies for other members of the mint family and offers an important foundation for decoding the synthetic pathways of bioactive compounds in medicinal plants. | Qing Zhao Jun Yang Meng-Ying Cui Jie Liu Yumin Fang Mengxiao Yan Wenqing Qiu Huiwen Shang Zhicheng Xu Reheman Yidiresi Jing-Ke Weng Tomas Pluskal Marielle Vigouroux Burkhard Steuernagel Yukun Wei Lei Yang Yonghong Hu Xiao-Ya Chen Cathie Martin | 2019 | Molecular Plant2019，12，7： | 23 |
| 2 | Surgical Management of Retinal Detachment Resulting from Macular Hole in a Setting of High Myopia显示文摘Purpose:To evaluate the anatomical and visual outcomes of pars plana vitrectomy (PPV) with internal limiting membrane (ILM).peeling and use of retinal tamponade for retinal detachments resulting from macular hole (MHRD) in highly myopic eyes. Methods:.Twenty-nine highly myopic patients.(29 eyes) underwent PPV with ILM peeling and retinal tamponade for MHRD were enrolled. Demographics and best-corrected visual acuity.(BCVA).were measured preoperatively and at final follow-up. Anatomical success and macular hole closure were analyzed. Results:.Patients' mean age of patients was 58.7±10.6 years, mean follow-up was 11.7±7.4 months. Twenty three eyes (23/26,88.5%)undergoing primary PPV combined with ILM peeling had successful initial retinal reattachment, including 19 eyes (19/19, 100%) with silicone oil tamponade and in 4 eyes (4/7,57.1%).with sulfur hexafluoride.(C3F8).tamponade. Overall anatomical success was achieved in 27 eyes (27/29, 93.1%)..The macular hole closure was observed in 17 eyes (17/26, 65.4%) with final anatomical success. Compared to preoperative BCVA,.the mean postoperative BCVA in the eyes with anatomical success was significantly improved (P = 0.007, Wilcoxon signed rank test). Conclusion:.As a primary or secondary procedure,.PPV combined with ILM peeling and usage of retinal tamponade serves as an effective method for MHRD in highly myopic eyes. | Liguo Feng Xiaohong Jin Jiuke Li Jing Zhai Wei Fang Jianfeng Mo Qirong Feng Yumin Li | 2012 | Eye Science2012，27，2： | 7 |
| 3 | Data mining-based study on sub-mentally healthy state among residents in eight provinces and cities in China显示文摘OBJECTIVE: To apply data mining methods to research on the state of sub-mental health among residents in eight provinces and cities in China and to mine latent knowledge about many conditions through data mining and analysis of data on 3970 sub-mentally healthy individuals selected from 13385 relevant question naires.METHODS: The strategic tree algorithm was used to identify the main mani festations of the state of sub-mental health. The backpropogation artificial neural network was used to analyze the main mani festations of sub-healthy mental states of three different degrees. A sub-mental health evaluation model was then established to achieve predictive evaluationresults.RESULTS: Using classifications from the Scale of Chinese Sub-healthy State, the main manifestations of sub-mental health selected using the strate gictree were F1101(Do you lack peace of mind?),F1102(Are you easily nervous when something comes up?), and F1002(Do you often sigh?). The relative intensity of manifestations of sub-mental health was highest for F1101, followed by F1102,and then F1002. Through study of the neural network, better differentiation could be made between moderate and severe and between mild and severe states of sub-mental health. The differentiation between mild and moderate sub-mental health states was less apparent. Additionally, the sub-mental health state evaluation model, which could be used to predict states of sub-mental health of different individuals, was established using F1101, F1102, F1002, and the mental self-assessment totals core.CONCLUSION: The main manifestations of the state of sub-mental health can be discovered using data mining methods to research and analyze the latent laws and knowledge hidden in research evidence on the state of sub-mental health. The state of sub-mental health of different individuals can be rapidly predicted using the model established here.This can provide a basis for assessment and intervention for sub-mental health. It can also replace the relatively outdated approaches to research on sub-health in the technical era of information and digitization by combining the study of states of sub-mental health with information techniques and by further quantifying the relevant information. | Hongmei Ni Xuming Yang Chengquan Fang Yingying Guo Mingyue Xu Yumin He | 2014 | Journal of Traditional Chinese Medicine2014，34，4： | 3 |
| 4 | SbMYB3 transcription factor promotes root-specific f lavone biosynthesis in Scutellaria baicalensis显示文摘Scutellaria baicalensis Georgi produces abundant root-specific f lavones(RSFs),which provide various benefits to human health.We have elucidated the complete biosynthetic pathways of baicalein and wogonin.However,the transcriptional regulation of f lavone biosynthesis in S.baicalensis remains unclear.We show that the SbMYB3 transcription factor functions as a transcriptional activator involved in the biosynthesis of RSFs in S.baicalensis.Yeast one-hybrid and transcriptional activation assays showed that SbMYB3 binds to the promoter of flavone synthase II-2(SbFNSII-2)and enhances its transcription.In S.baicalensis hairy roots,RNAi of SbMYB3 reduced the accumulation of baicalin and wogonoside,and SbMYB3 knockout decreased the biosynthesis of baicalein,baicalin,wogonin,and wogonoside,whereas SbMYB3 overexpression enhanced the contents of baicalein,baicalin,wogonin,and wogonoside.Transcript profiling by qRT–PCR demonstrated that SbMYB3 activates SbFNSII-2 expression directly,thus leading to more abundant accumulation of RSFs.This study provides a potential target for metabolic engineering of RSFs. | Yumin Fang Jie Liu Minmin Zheng Sanming Zhu Tianlin Pei Mengying Cui Lijing Chang Hanwen Xiao Jun Yang Cathie Martin Qing Zhao | 2023 | Horticulture Research2023，10，2： | 2 |
| 5 | Synthesis of pyridine and picolines over Co - modified HZSM - 5 catalyst 显示文摘 | Yumin Heqin Yang Fang Jin Yue Zhang Yongdan Li | 2008 | Chemical Engineering Journal2008，136，： | 1 |
| 6 | MicroRNA-124–Mediated Regulation of Inhibitory Member of Apoptosis-Stimulating Protein of p53 Family in Experimental Stroke显示文摘 | Xiangrong Liu Fang Li Shangfeng Zhao Yumin Luo Jun Kang Haiping Zhao Feng Yan Sijie Li Xunming Ji | 2013 | Stroke2013，，7： | 1 |
| 7 | How to secure multi-domain wireless mesh显示文摘 | ZHU Xiaoyan FANG Yuguang WANG Yumin | 2010 | Wireless Networks2010，16，5： | 1 |
| 8 | Carbon monoxide releasing molecule?2 attenuated ischemia/reperfusion?inducedapoptosis in cardiomyocytes via a mitochondrial pathway显示文摘 | Shen Zhao Qingming Lin Heng Li Yumin He Xiangshao Fang Feng Chen Changwei Chen Zitong Huang | 2014 | Molecular Medicine Reports2014，，2： | 1 |
| 9 | Effect of Different Drying Processes on the Protein Degradation and Sensory Quality of Layú: A Chinese Dry-Curing Grass Carp显示文摘 | Jinjie Zhang Zhongxiang Fang Yumin Cao Yuting Xu Yaqin Hu Xingqian Ye Wenge Yang | 2013 | 2013 (13-1)2013，，13： | 1 |
| 10 | Genetic Architecture and Functional Implications of the CSF‑Contacting Nucleus显示文摘We previously identified a unique nucleus,the cerebrospinal fluid(CSF)-contacting nucleus.This study aims to understand its gene architecture and preliminarily suggest its functions.The results showed that there were about 19,666 genes in this nucleus,of which 913 were distinct from the dorsal raphe nucleus(non-CSF contacting).The top 40 highly-expressed genes are mainly related to energy metabolism,protein synthesis,transport,secretion,and hydrolysis.The main neurotransmitter is 5-HT.The receptors of 5-HT and GABA are abundant.The channels for Cl–,Na+,K+,and Ca2+are routinely expressed.The signaling molecules associated with the CaMK,JAK,and MAPK pathways were identified accurately.In particular,the channels of transient receptor potential associated with nociceptors and the solute carrier superfamily members associated with cell membrane transport were significantly expressed.The relationship between the main genes of the nucleus and life activities is preliminarily verified. | Siyuan Song Yumin Yuan Lingling Xu Jun Jiang Ying Li Yao Yan Qing Li Fang Zhou Junli Cao Licai Zhang | 2023 | Neuroscience Bulletin2023，39，11： | 1 |
| 11 | A Fast Element-Free Galerkin Method for 3D Elasticity Problems显示文摘In this paper,a fast element-free Galerkin(FEFG)method for three-dimensional(3D)elasticity problems is established.The FEFG method is a combination of the improved element-free Galerkin(IEFG)method and the dimension splitting method(DSM).By using the DSM,a 3D problem is converted to a series of 2D ones,and the IEFG method with a weighted orthogonal function as the basis function and the cubic spline function as the weight function is applied to simulate these 2D problems.The essential boundary conditions are treated by the penalty method.The splitting direction uses the finite difference method(FDM),which can combine these 2D problems into a discrete system.Finally,the system equation of the 3D elasticity problem is obtained.Some specific numerical problems are provided to illustrate the effectiveness and advantages of the FEFG method for 3D elasticity by comparing the results of the FEFG method with those of the IEFG method.The convergence and relative error norm of the FEFG method for elasticity are also studied. | Zhijuan Meng Yanan Fang Yumin Cheng | 2022 | Computer Modeling in Engineering & Sciences2022，，7： | 0 |
| 12 | Gap-free genome assembly and CYP450 gene family analysis reveal the biosynthesis of anthocyanins in Scutellaria baicalensis显示文摘Scutellaria baicalensis Georgi,a member of the Lamiaceae family,is a widely utilized medicinal plant.The flavones extracted from S.baicalensis contribute to numerous health benefits,including anti-inflammatory,antiviral,and anti-tumor activities.However,the incomplete genome assembly hinders biological studies on S.baicalensis.This study presents the first telomere-to-telomere(T2T)gap-free genome assembly of S.baicalensis through the integration of Pacbio HiFi,Nanopore ultra-long and Hi-C technologies.A total of 384.59 Mb of genome size with a contig N50 of 42.44 Mb was obtained,and all sequences were anchored into nine pseudochromosomes without any gap or mismatch.In addition,we analysed the major cyanidin-and delphinidin-based anthocyanins involved in the determination of blue-purple flower using a widely-targeted metabolome approach.Based on the genome-wide identification of Cytochrome P450(CYP450)gene family,three genes(SbFBH1,2,and 5)encoding flavonoid 3′-hydroxylases(F3′Hs)and one gene(SbFBH7)encoding flavonoid 3′5′-hydroxylase(F3′5′H)were found to hydroxylate the B-ring of flavonoids.Our studies enrich the genomic information available for the Lamiaceae family and provide a toolkit for discovering CYP450 genes involved in the flavonoid decoration. | Tianlin Pei Sanming Zhu Weizhi Liao Yumin Fang Jie Liu Yu Kong Mengxiao Yan Mengying Cui Qing Zhao | 2023 | Horticulture Research2023，10，12： | 0 |
| 13 | A landslide extraction method of channel attention mechanismU-Net network based on Sentinel-2A remote sensing images显示文摘Accurate landslide extraction is significant for landslide disaster prevention and control.Remote sensing images have been widely used in landslide investigation,and landslide extraction methods based on deep learning combined with remote sensing images(such as U-Net)have received a lot of attention.However,because of the variable shape and texture features of landslides in remote sensing images,the rich spectral features,and the complexity of their surrounding features,landslide extraction using U-Net can lead to problems such as false detection and missed detection.Therefore,this study introduces the channel attention mechanism called the squeeze-and-excitation network(SENet)in the feature fusion part of U-Net;the study also constructs an attention U-Net landside extraction model combining SENet and U-Net,and uses Sentinel-2A remote sensing images for model training and validation.The extraction results are evaluated through different evaluation metrics and compared with those of two models:U-Net and U-Net Backbone(U-Net Without Skip Connection).The results show that proposed the model can effectively extract landslides based on Sentinel-2A remote sensing images with an F1 value of 87.94%,which is about 2%and 3%higher than U-Net and U-Net Backbone,respectively,with less false detection and more accurate extraction results. | Hesheng Chen Yi He Lifeng Zhang Sheng Yao Wang Yang Yumin Fang Yaoxiang Liu Binghai Gao | 2023 | International Journal of Digital Earth2023，16，1： | 0 |
| 14 | Legionella pneumonia complicated with rhabdomyolysis and acute kidney injury diagnosed by metagenomic nextgeneration sequencing: a case report显示文摘The triad of Legionella pneumonia,rhabdomyolysis,and acute kidney injury(AKI)is uncommon but can be devastating.[1]It is vital to recognize Legionella species early as a potential cause of pneumonia.Legionella infection remains underdiagnosed because of the nonspecific nature of clinical features and the shortcomings of routinely available diagnostic tests.[2]Metagenomic next-generation sequencing(mNGS)is a culture-free and hypothesis-free diagnostic method that can rapidly identify almost all known pathogens in a sample.[3]Here,we present a successfully cured case of rhabdomyolysis and AKI caused by Legionella pneumophila with etiologic diagnosis aided by the use of mNGS. | Bin Deng Junjie Hua Yufei Zhou Duoxian Zhan Lijuan Zhu Yixing Zhan Yumin Fang Yun Ji Libin Li | 2023 | World Journal of Emergency Medicine2023，14，4： | 0 |
| 15 | Breeding and application of Jiafuzhan,a new elite early indica rice cultivar in China显示文摘After 20 years of dedicated research,Jiafuzhan has been successfully developed under the new technologies in breeding high-quality early indica rice cultivars.Its rice quality has almost reached the A-level Editable Rice of Agriculture Department of China,and its average production reaches 400-500 kg/(666.7 m^(2)).This new cultivar also has other characteristics such as enhanced resistance of blast and fallen,steady productivity,and strong adaptability.Jiafuzhan has been put into production of over 11.4×10^(4)hm^(2)in Fujian Province and has been introduced and extended in other Provinces like Jiangxi,Guangdong,and Guangxi,China.The successes of breeding Jiafuzhan is a solution to the existing perennial problems in the rice industry,such as poor grain quality of big-grain rice and early indica rice,low productivity,and poor blast resistance of elite rice. | WANG Houcong HUANG Huakang QIU Simi ZHANG Shi FANG Yashun CAI Jinlei ZHENG Xuan HUANG Yumin CHEN Ruming SUN Chuanzhi CHEN Shuanglong CHI Xiaowen LIAO Xinying ZHANG Weiqing ZHONG Xinbin | 2007 | Frontiers in Biology2007，2，2： | 0 |
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