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| 1 | Antimicrobial activity of Sapindus mukorossi and Rheum emodi extracts against H pylori: In vitro and in vivo studies显示文摘AIM: To evaluate the antibacterial activity of Sapindus mukorossi (S. mukorossi) and Rheum emodi (R. emodi). METHODS: Powders of S. mukorossi and R. emodi were extracted successively with petroleum ether, benzene, chloroform and ethanol and were concentrated in vacuum. The disk diffusion method was used for in vitro studies and in vivo studies were performed on male Wister rats. Thirty resistant clinical isolates of H pylori, as determined by their antibiotic sensitivity patterns by E-test, along with two Gram +ve (S. aureus, B. subtilis) and two Gram -ve (E. coli, P. vugaris) organisms were screened for their susceptibility patterns against these extracts. RESULTS: In our screening, all 30 resistant isolates and the other four organisms (two Gram +ve S. aureus, B. subtilis and two Gram -ve, E. coli, P. vugaris) were sensitive to the test compounds. It was found that ethanol and chloroform extracts of S. mukorossi and ethanol and benzene extracts of R. emodi inhibited H pylori at very low concentrations. In the in vitro study, the isolates showed a considerable zone of inhibition at very low concentrations (10 μg/mL) for both the extracts. In the in vivo study, the H pylori infection was cleared with minimal doses of extracts of S. mukorossi (2.5 mg/mL) and R. emodi (3.0 mg/mL) given orally for seven days. CONCLUSION: We can conclude from this study that the extracts of S. mukorossi and R. emodi inhibited the growth of pylori in vitro and, in in vivo studies, the H pylori infection cleared within seven days at very low concentrations. We also found that H pylori did not acquire resistance against these herbal extracts even after 10 consecutive passages. | Mohammed Ibrahim Aleem A Khan Santosh K Tiwari Mohammed Aejaz Habeeb MN Khaja CM Habibullah | 2006 | World Journal of Gastroenterology2006，12，44： | 46 |
| 2 | Hepatoprotective activity of Sapindus mukorossi and Rheum emodi extracts: In vitro and in vivo studies显示文摘AIM: To study the hepatoprotective capacity of Sapindus mukorossi (S. mukorossi) and Rheum emodi (R. emodi) extracts in CCl4 treated male rats. METHODS: The dried powder of S. mukorossi and R. emodi was extracted successively with petroleum ether, benzene, chloroform, and ethanol and concentrated in vacuum. Primary rat hepatocyte monolayer cultures were used for in vitro studies. In vivo, the hepatoprotective capacity of the extract of the fruit pericarp of S. mukorossi and the rhizomes of R. emodi was analyzed in liver injured CCl4-treated male rats. RESULTS: In vitro: primary hepatocytes monolayer cultures were treated with CCl4 and extracts of S. mukorossi & R. emodi. A protective activity could be demonstrated in the CCl4 damaged primary monolayer culture. In vivo : extracts of the fruit pericarp of S. mukorossi (2.5 mg/mL) and rhizomes of R. emodi (3.0 mg/mL) were found to have protective properties in rats with CCl4 induced liver damage as judged from serum marker enzyme activities. CONCLUSION: The extracts of S. mukorossi and R. emodi do have a protective capacity both in vitro on primary hepatocytes cultures and in in vivo in a rat model of CCl4 mediated liver injury. | Mohammed Ibrahim Mohammed Nane Khaja Anjum Aara Aleem Ahmed Khan Mohammed Aejaz Habeeb Yalavarthy Prameela Devi Mangamoori Lakshmi Narasu Chitoor Mohammed Habibullah | 2008 | World Journal of Gastroenterology2008，14，16： | 24 |
| 3 | Characterization of hepatic progenitors from human fetal liver during second trimester显示文摘AIM: To enrich hepatic progenitors using epithelial cell adhesion molecule (EpCAM) as a marker from human fetal liver and investigate the expression of human leukocyte antigen (HLA) and their markers associated with hepatic progenitor cells. METHODS: EpCAM +ve cells were isolated using magnetic cell sorting (MACS) from human fetuses (n = 10) at 15-25 wk gestation. Expression of markers for hepatic progenitors such as albumin, alpha-fetoprotein (AFP), CD29 (integrin β1), CD49f (integrin α6) and CD90 (Thy 1) was studied by using flow cytometry, immunocytochemistry and RT-PCR; HLA class Ⅰ (A, B, C) and class Ⅱ (DR) expression was studied by flow cytometry only. RESULTS: FACS analysis indicated that EpCAM +ve cells were positive for CD29, CD49f, CD90, CD34, HLA class Ⅰ, albumin and AFP but negative for HLA class Ⅱ (DR) and CD45. RT PCR showed that EpCAM +ve cells expressed liver epithelial markers (CK18), biliary specific marker (CK19) and hepatic markers (albumin, AFP). On immunocytochemical staining, EpCAM +ve cells were shown positive signals for CK18 and albumin. CONCLUSION: Our study suggests that these EpCAM +ve cells can be used as hepatic progenitors for cell transplantation with a minimum risk of alloreactivity and these cells may serve as a potential source for enrichment of hepatic progenitor. | Mekala Subba Rao Aleem Ahmed Khan Nyamath Parveen Mohammed Aejaz Habeeb Chittoor Mohammed Habibullah Gopal Pande | 2008 | World Journal of Gastroenterology2008，14，37： | 16 |
| 4 | Characterization of hepatic progenitors from human fetal liver using CD34 as a hepatic progenitor marker显示文摘AIM: To enrich putative hepatic progenitors from the developing human fetal liver using CD34 as a marker. METHODS: Aborted fetuses of 13-20 wk were used for the isolation of liver cells. The cells were labeled with anti CD34; a marker used for isolating progenitor population and the cells were sorted using magnetic cell sorting. The positive fractions of cells were assessed for specific hepatic markers. Further, these cells were cultured in vitro for long term investigation. RESULTS: Flow cytometric and immunocytochemical analysis for alphafetoprotein (AFP) showed that the majority of the enriched CD34 positive cells were positive for AFP. Furthermore, these enriched cells proliferated in the long term and maintained hepatic characteristics in in vitro culture. CONCLUSION: The study shows that aborted human fetal liver is a potential source for isolation of hepatic progenitors for clinical applications. The study also demonstrates that CD34 can be a good marker for the enrichment of progenitor populations. | Parveen Nyamath Ayesha Alvi Aejaz Habeeb Sanjeev Khosla Aleem A Khan CM Habibullah | 2007 | World Journal of Gastroenterology2007，13，16： | 9 |
| 5 | Hepatic stem cells: A viable approach for the treatment of liver cirrhosis显示文摘Liver cirrhosis is characterized by distortion of liver architecture, necrosis of hepatocytes and regenerative nodules formation leading to cirrhosis. Various types of cell sources have been used for the management and treatment of decompensated liver cirrhosis.Knowledge of stem cells has offered a new dimension for regenerative therapy and has been considered as one of the potential adjuvant treatment modality in patients with end stage liver diseases(ESLD). Human fetal hepatic progenitor cells are less immunogenic than adult ones. They are highly propagative and challenging to cryopreservation. In our earlier studies we have demonstrated that fetuses at 10-18 wk of gestation age contain a large number of actively dividing hepatic stem and progenitor cells which possess bipotent nature having potential to differentiate into bile duct cells and mature hepatocytes. Hepatic stem cell therapy for the treatment of ESLD is in their early stage of the translation. The emerging technology of decellularization and recellularization might offer a significant platform for developing bioengineered personalized livers to come over the scarcity of desired number of donor organs for the treatment of ESLD. Despite these significant advancements long-term tracking of stem cells in human is the most important subject nowadays in order to answer several unsettles issues regarding the route of delivery, the choice of stem cell type(s), the cell number and the timepoint of cell delivery for the treatment in a chronic setting. Answering to these questions will further contribute to the development of safer, noninvasive, and repeatable imaging modalities that could discover better cell therapeutic approaches from bench to bedside. Combinatorial approach of decellularization and nanotechnology could pave a way towards the better understanding in determination of cell fate posttransplantation. | Md Aejaz Habeeb Sandeep Kumar Vishwakarma Avinash Bardia Aleem Ahmed Khan | 2015 | World Journal of Stem Cells2015，7，5： | 5 |
| 6 | A review of upper limb injuries in bear maul victims: Consistent pattern and inverse relation in severity with facial and scalp injuries显示文摘PurposeBear 虐待损害最普通的野生的动物被造成在印度的损害。超过 300 熊每在 1 年的经期上的连续病人为熊的整形外科的管理向我们的部门汇报了的 year.MethodsTwenty 一个虐待损害报告到我们的医院虐待损害。所有病人也从外部医院被提交或从我们的 hospital.ResultsAll 的另外的外科的部门,病人有可变严厉的美容 / 头皮的损害。在所有病人,手足和面部损伤的严厉是互成反比的。在大多数病人的上面的手足损伤的模式是类似的。有的任何一个远侧的肱骨或中间的 shaft/proximal 前臂骨头骨折折断的十五个病人。二有的远侧的前臂骨头破裂, 2 有的 carpal/metacarpal 破裂和 1 块有的锁骨断裂。仅仅, 1 有更低的手足骨折。13 从 21 个病人联系了深奥手足的神经与血管的损害。典型特征是病人们通常有的在熊的损害虐待的影响 limb.ConclusionUpper 手足的广泛的软织物参与类似的模式。上面的手足和美容 / 头皮的损伤的严厉是互成反比的。多级式的整形外科的手术为如此的复杂手足损害被需要。 | Towseef Ahmad Bhat Amara Gulzar Aejaz Ahmad Bhat Tariq Ahmad Bhat Zameer Ali | 2018 | Chinese Journal of Traumatology2018，21，1： | 3 |
| 7 | Diffusion-weighted MRI in cervical cancer显示文摘 | Patrick Z. McVeigh Aejaz M. Syed Michael Milosevic Anthony Fyles Masoom A. Haider | 2008 | European Radiology2008，，5： | 3 |
| 8 | Helicobacter pylori and other Helicobacter species DNA in human bile samples from patients with various hepato-biliary diseases显示文摘瞄准:为了由 16S rRNA 基因的嵌套的 PCR 调查 Helicobacter 种类的存在,由 Helicobacter pylori (H pylori ) 的存在跟随了 16S rRNA,脲,在获得在的胆汁的 cagA 基因内视镜后退从 60 印第安人的 cholangio-pancreatography (ERCP ) 使遭到。方法:六十件胆汁样品从在 ERCP 与各种各样的 hepato 胆汁的疾病和控制题目诊断的病人被获得。PCR 分析为 Helicobacter 类 16S rRNA 基因和 H pylori (16S rRNA,脲和 cagA ) 用教材被执行基因。胃的 H pylori 地位也从与各种各样的 hepato 胆汁的疾病和控制从病人在内视镜检查法获得的活体检视被估计。控制组主要与胃的混乱由题目组成了。定序分析被执行证实有 16S rRNA 和 cagA 教材的 PCR 产品从 H pylori 被导出。结果没有 Helicobacters 从胆汁样品在文化被种。Helicobacter DNA 在 96.7% 组和 6.6% 的胆汁被检测我和 II 分别地。十来自组,我为 16S rRNA 和脲是积极的, 9 为 cagA 是积极的基因。在从控制的 2 的对比, 1 与 16S 放大了教材使用了的 rRNA,脲和 cagA。16S rRNA 基因和 cagA 的序列是 99% 类似于 Helicobacter pylori。结论:Helicobacters 与各种各样的 hepato 胆汁的混乱的致病被联系。 | Santosh K Tiwari Aleem A Khan Mohd Ibrahim Mohd Aejaz Habeeb C M Habibullah | 2006 | World Journal of Gastroenterology2006，12，14： | 2 |
| 9 | 一例罕见的子宫原始神经外胚层瘤病例报道(英文)显示文摘Primitive neuroectodermal tumor (PNET) of uterus is the rare of the rarest among the tumors of female genital tract. We herein reported a 32 years old female operated for lower abdominal lump,which was diagnosed,as PNET later confirmed by immunohistochemistry (IHC). She was lost to follow-up and presented 10 months later with recurrence. She was treated with cyclical combination chemotherapy followed by definitive surgery and is in complete remission at present. | Syed Nisar Ahmad Shiekh Aejaz Aziz Rangreze Imran Manzoor Ahmad Banday Gul Mohammad A.R.Lone Parveen Reshi Nisar Ahmad Wani Shaheena Parveen | 2010 | The Chinese-German Journal of Clinical Oncology2010，9，6： | 2 |
| 10 | Overview of purple blotch disease and understanding its management through chemical, biological and genetic approaches显示文摘Purple blotch disease of Allium spp. crops caused by Alternaria porri has remained a major concern in agriculture for both farmers and research fraternity as it severely damages the crops and drastically reduces the yield. The symptoms appear after 1–4 days of infection and bulb rot begin, and eventually turn into dark reddish-purple and then brownish/black lesions. Many factors like season, time of sowing, humidity and temperature, stage of crop, and plant architecture have a huge impact on the progression of purple blotch disease. Many genic markers based on amplification of an Alta1 gene sequence have been designed for identification and differentiation of different Alternaria species groups. Among the most commonly used fungicides, mancozeb, tebuconazole, difenaconazole and azoxystrobin were found to be the ideal for the management of purple blotch disease and increased garlic yield. Many biological approaches such as plant extracts and bio-control agents were found partially effective for controlling the disease. A report on QTL mapping for purple blotch resistance discovered that purple blotch resistance is controlled by a single dominant gene ApR1. To completely understand the purple blotch disease resistance for crop improvement, a study is required at transcriptome level for hunting purple blotch resistant genes by gene annotation and mining. Genetic engineering and genome editing are other approaches that can be done for engineering disease resistance in Allium crops for genetic improvement. | Aejaz Ahmad DAR Susheel SHARMA Reetika MAHAJAN Muntazir MUSHTAQ Ankila SALATHIA Shahid AHAMAD Jag Paul SHARMA | 2020 | Journal of Integrative Agriculture2020，19，12： | 2 |
| 11 | Macrophage migration inhibitory factor, Toll-like receptor 4, and CD14 polymorphisms with altered expression levels in patients with ulcerative colitis显示文摘 | Gunisetty Sivaram Santosh K. Tiwari Avinash Bardia Farha Anjum Satti Vishnupriya Aejaz Habeeb Aleem A. Khan | 2012 | Human Immunology2012，，2： | 1 |
| 12 | Functional polymorphisms in XRCC - 1 and APE - 1 contribute to increased apoptosis and risk of ulcerative colitis显示文摘 | Avinash Bardia Santosh K. Tiwari Sivaram Gunisetty Farha Anjum Pratibha Nallari Md. Aejaz Habeeb Aleem A. Khan | 2012 | Inflammation Research2012，，4： | 1 |
| 13 | Estrogen receptor Ainhibits p53 - mediated transcriptional repression: Implications forthe regulation of apoptosis显示文摘 | Aejaz Sayeed Santhi DK Wensheng L | 2007 | Cancer Res2007，67，16： | 1 |
| 14 | Diffusion-weighted MRI in cervical cancer显示文摘 | Patrick Z. McVeigh Aejaz M. Syed Michael Milosevic Anthony Fyles Masoom A. Haider | 2008 | European Radiology2008，，5： | 1 |
| 15 | Bioengineered functional humanized livers: An emerging supportive modality to bridge the gap of organ transplantation for management of end-stage liver diseases显示文摘End stage liver diseases(ESLD) represent a major, neglected global public health crisis which requires an urgent action towards finding a proper cure. Orthotropic liver transplantation has been the only definitive treatment modality for ESLD. However, shortage of donor organs, timely unavailability, post-surgery related complications and financial burden on the patients limits the number of patients receiving the transplants. Since last two decades cell-based therapies have revolutionized the field of organ/tissue regeneration. However providing an alternative organ source to address the donor liver shortage still poses potential challenges. The developments made in this direction provide useful futuristic approaches, which could be translated into preclinical and clinical settings targeting appropriate app-lications in specific disease conditions. Earlier studieshave demonstrated the applicability of this particularapproach to generate functional organ in rodent systemby connecting them with portal and hepatic circulatorynetworks. However, such strategy requires very highlevel of surgical expertise and also poses the technicaland financial questions towards its future applicability.Hence, alternative sites for generating secondary organsare being tested in several types of disease conditions.Among different sites, omentum has been proved tobe more appropriate site for implanting several kindsof functional tissue constructs without eliciting muchimmunological response. Hence, omentum may be considered as better site for transplanting humanized bioengineered ex vivo generated livers, thereby creatinga secondary organ at intra-omental site. However, theexpertise for generating such bioengineered organs arelimited and only very few centres are involved for investigating the potential use of such implants in clinicalpractice due to gap between the clinical transplantsurgeons and basic scientists working on the conceptevolution. Herein we discuss the recent advances andchallenges to create functional secondary organs through intraomental transplantation of ex vivo generated bioengineered humanized livers and their furtherapplication in the management of ESLD as a supportivebridge for organ transplantation. | Sandeep Kumar Vishwakarma Chandrakala Lakkireddy Avinash Bardia Syed Ameer Basha Paspala Chaturvedula Tripura Md Aejaz Habeeb Aleem Ahmed Khan | 2018 | World Journal of Hepatology2018，10，11： | 1 |
| 16 | Diffusion-weighted MRI in cervical cancer显示文摘 | Patrick Z. McVeigh Aejaz M. Syed Michael Milosevic Anthony Fyles Masoom A. Haider | 2008 | European Radiology2008，，5： | 1 |
| 17 | Relevance of Helicobacter pylori genotypes in gastric pathology and its association with plasma malondialdehyde and nitric oxide levels显示文摘 | Santosh K. Tiwari G. Manoj Vishwas Sharma G. Sivaram R. Saikant Avinash Bardia Varun K. Sharma Zakia Abid Aleem A. Khan M. Aejaz Habeeb C. M. Habibullah B. Santhosh Kumar Amrita Nandan | 2010 | Inflammopharmacology2010，，2： | 1 |
| 18 | Intestinal ascariasis: the commonest cause of bowel obstruction in children at a tertiary care center in Kashmir显示文摘 | Aejaz Ahsan Baba Syed Mudasir Ahmad Khursheed Ahmad Sheikh | 2009 | Pediatric Surgery International2009，，12： | 1 |
| 19 | Stem cell therapy- present status显示文摘 | Aejaz HM Aleem AK Parveen N | 2007 | Transplant Proc2007，39，3： | 1 |
| 20 | Diffusion-weighted MRI in cervical cancer显示文摘 | Patrick Z. McVeigh Aejaz M. Syed Michael Milosevic Anthony Fyles Masoom A. Haider | 2008 | European Radiology2008，，5： | 1 |
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