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| 1 | CRISPR-Cas9-mediated multiplex gene editing in CAR-T cells显示文摘 | Xiaojuan Liu Yongping Zhang Chen Cheng Albert W Cheng Xingying Zhang Na Li Changqing Xia Xiaofei Wei Xiang Liu Haoyi Wang | 2017 | Cell Research2017，27，1： | 40 |
| 2 | Structure and function of aggrecan显示文摘Aggrecan is the major proteoglycan in the articular cartilage. This molecule is important in the proper functioning of articular cartilage because it provides a hydrated gel structure (via its interaction with hyaluronan and link protein) that endows the cartilage with load-bearing properties. It is also crucial in chondroskeletal morphogenesis during development. Aggrecan is a multimodular molecule expressed by chondrocytes. Its core protein is composed of three globular domains (G1, G2, and G3) and a large extended region (CS) between G2 and G3 for glycosaminoglycan chain attachment. G1 comprises the amino terminus of the core protein. This domain has the same structural motif as link protein. Functionally, the G1 domain interacts with hyaluronan acid and link protein, forming stable ternary complexes in the extracellular matrix. G2 is homologous to the tandem repeats of G1 and of link protein and is involved in product processing. G3 makes up the carboxyl terminus of the core protein. It enhances glycosaminoglycan modification and product secretion. Aggrecan plays an important role in mediating chondrocyte-chondrocyte and chondrocyte-matrix interactions through its ability to bind hyaluronan. | CHRIS KIANI, LIWEN CHEN, YAO JIONG WU, ALBERT J YEE, BURTON B YANG, Sunnybrook and Women’s College Health Sciences Centre and Department of Laboratory Medicine and Patobiology, 2 Department of Surgeny, Faculty of Medicine, University of Toronto, Canada | 2002 | Cell Research2002，12，1： | 27 |
| 3 | Histone tails regulate DNA methylation by allosterically activating de novo methyltransferase显示文摘genomic DNA 的 Cytosine methylation 控制基因表示并且维持染色体稳定性。一个特定的 DNA 序列怎么被 methyltransferase 为 methylation 指向,大部分是未知的。这里,我们证明 histone H3 跟踪缺乏离氨酸 4 (K4 ) methylation 功能作为为由到它的植物 homeodomain (哲学博士) 的绑定的 methyltransferase Dnmt3a 的 allosteric 使活跃之物。在 vitro, histone H3 肽相对地刺激了 Dnmt3a 的 methylation 活动直到 8 褶层,以一种方式与 K4 methylation 的水平相关。allosteric 规定的生物意义被在 PHD 和其变化由 H3 肽然而并非 H3 肽的绑定损害了 methylation 活动的刺激的 Dnmt3a 的催化领域的分子的建模和关键残余的鉴定表明。显著地,这些变异的 Dnmt3a 蛋白质在 DNA methylation 是几乎不活跃的当他们到 genomic 的招募指向时,什么时候在老鼠表示了胚胎的干细胞,是未改变的。我们因此为 de novo DNA methylation 建议二拍子的圆舞机制 - 染色质绑定或 DNA 有约束力的因素可能帮助的 methyltransferase 的第一招募,然后取决于在 Dnmt3a 和 histone 之间的相互作用的 allosteric 激活跟踪 - 后者可能为 methylation 活动用作一个检查点。 | Bin-Zhong Li Zheng Huang Qing-Yan Cui Xue-Hui Song Lin Du Albert Jeltsch Ping Chen Guohong Li En Li Guo-Liang Xu | 2011 | Cell Research2011，21，8： | 17 |
| 4 | Analysis of inborn errors of metabolism: disease spectrum for expanded newborn screening in Hong Kong显示文摘氨基酸,器官的酸和丰满的酸氧化的新陈代谢(IEM ) 的古典先天的错误的背景数据大部分正在缺乏香港,在集体基于 spectrometry 的扩展新生儿为 IEM 屏蔽没被开始的地方。瞄准在香港评估近似发生,光谱和古典 IEM 的另外的特征的当前的学习,将在开发为实验室血浆氨基酸记录的本地 area.Methods 屏蔽节目的一个扩展新生儿重要,血浆 acylcarnitines 和尿从年 2005~2009 的器官的酸分析在为 IEM 提供生物化学、基因的诊断服务的三个地区性的化学病理实验室包括回顾地 reviewed.Resu 在香港的古典 IEM 的发生粗略地被估计每 1000live 出生每 4122 生活出生,或 0.243 个盒子是至少 1 个盒子。这发生类似于全球报导的那些,包括中国的大陆。hyperphenylalaninemia 的估计的发生在 29 542 实时 births.Conclusions 是 1 我们的数据显示它为在香港的扩展新生的屏蔽节目的介绍是无可争辩的。因为香港是一个大城市的城市,一个全面扩展新生的屏蔽程序和工作分派系统应该是可得到的服务在区域出生的出生不满一月的婴儿。 | Han-Chih Hencher Lee Chloe Miu Mak Ching-Wan Lam Yuet-Ping Yuen, Angel On-Kei Chan Chi-Chung Shek Tak-Shing Siu Chi-Kong Lai Chor-Kwan Ching Wai-Kwan Siu Sammy Pak-Lam Chen Chun-Yiu Law Morris Hok-Leung Tai Sidney Tam Albert Yan-Wo Chan | 2011 | Chinese Medical Journal2011，，7： | 12 |
| 5 | Thermal-responsive genetic and epigenetic regulation of DAM cluster controlling dormancy and chilling requirement in peach floral buds显示文摘The Dormancy-associated MADS-box(DAM)gene cluster in peach serves as a key regulatory hub on which the seasonal temperatures act and orchestrate dormancy onset and exit,chilling response and floral bud developmental pace.Yet,how different temperature regimes interact with and regulate the six linked DAM genes remains unclear.Here,we demonstrate that chilling downregulates DAM1 and DAM3–6 in dormant floral buds with distinct patterns and identify DAM4 as the most abundantly expressed one.We reveal multiple epigenetic events,with tri-methyl histone H3 lysine 27(H3K27me3)induced by chilling specifically in DAM1 and DAM5,a 21-nt sRNA in DAM3 and a ncRNA induced in DAM4.Such induction is inversely correlated with downregulation of their cognate DAMs.We also show that the six DAMs were hypermethylated,associating with the production of 24-nt sRNAs.Hence,the chilling-responsive dynamic of the different epigenetic elements and their interactions likely define distinct expression abundance and downregulation pattern of each DAM.We further show that the expression of the five DAMs remains steadily unchanged or continuously downregulated at the ensuing warm temperature after chilling,and this state of regulation correlates with robust increase of sRNA expression,H3K27me3 and CHH methylation,which is particularly pronounced in DAM4.Such robust increase of repressive epigenetic marks may irreversibly reinforce the chillingimposed repression of DAMs to ensure flower-developmental programming free from any residual DAM inhibition.Taken together,we reveal novel information about genetic and epigenetic regulation of the DAM cluster in peach,which will be of fundamental significance in understanding of the regulatory mechanisms underlying chilling requirement and dormancy release,and of practical application for improvement of plasticity of flower time and bud break in fruit trees to adapt changing climates. | Hong Zhu Pao-Yang Chen Silin Zhong Chris Dardick Ann Callahan Yong-Qiang An Steve van Knocker Yingzhen Yang Gan-Yuan Zhong Albert Abbott Zongrang Liu | 2020 | Horticulture Research2020，7，1： | 9 |
| 6 | 巴曲酶对大鼠颞叶梗塞后学习障碍的改善作用显示文摘目的研究巴曲酶对大鼠左侧颞叶梗塞后空间学习障碍的改善作用。方法采用立体定向光化学诱导脑梗塞技术选择性地导致大鼠左侧颞叶皮层梗塞,术前30分钟及术后第3天分别给巴曲酶组大鼠腹腔注射巴曲酶8BU/kg,行为实验装置由Morris水迷宫及图像自动监视系统组成。结果(1)巴曲酶可以显著地缩短梗塞大鼠在Morris迷宫中搜索目标的平均反应时和行程;(2)巴曲酶组大鼠较多、较早地使用了正常的认知策略。结论巴曲酶对左侧颞叶梗塞大鼠空间认知功能障碍具有明显的改善作用。它除了溶栓作用外,还参与了神经损伤的修复过程。 | 杨炯炯 隋南 匡培梓 吴卫平 姜树军 匡培根 ALBERT CHEN | 1999 | 临床神经病学杂志1999，12，4： | 7 |
| 7 | Prognosis of lymphotropic invasive micropapillary breast carcinoma analyzed by using data from the National Cancer Database显示文摘Background:Invasive micropapillary carcinoma(IMPC)is an uncommon subtype of breast cancer.Previous studies of this subtype demonstrated a higher propensity for lymph node metastases as compared with invasive ductal carci-noma(IDC).The purpose of the present study was to determine the clinical characteristics,outcomes,and propensity for lymph node metastasis of patients with IMPC of the breast recorded in the National Cancer Database(NCDB).Methods:Records of patients with IMPC diagnosed between 2004 and 2014 were retrieved from the NCDB.Log-rank test was performed to evaluate associations of clinical characteristics with overall survival(OS).Cox proportional hazards model was used to determine variables associated with OS.Results:Overall,2660 patients with IMPC met the selection criteria;the 5-year OS rate was 87.5%and 24.9%of patients had nodal involvement at presentation.Patients with≥4 positive lymph nodes had shorter OS than node-negative patients,whereas patients with 1-3 positive nodes had similar OS to node-negative patients.Age<65 years,receipt of radiotherapy,and estrogen receptor positivity were also associated with prolonged OS.The benefit of radiotherapy was limited to IMPC patients undergoing lumpectomy;there was no benefit for the patients undergoing mastectomy(regardless of nodal positivity/negativity).Conclusions:Favorable prognostic factors of IMPC patients included age<65 years,<4 positive lymph nodes,receipt of radiotherapy,and estrogen receptor positivity.The results presented herein suggest a survival benefit asso-ciated with radiotherapy in IMPC treatment,though this may be limited to the patients treated with lumpectomy. | Gary D.Lewis Yan Xing Waqar Haque Tejal Patel Mary Schwartz Albert Chen Andrew Farach Sandra S.Hatch E.Brian Butler Jenny Chang Bin S.Teh | 2019 | Cancer Communications2019，39，1： | 7 |
| 8 | Silicon carbide resonant tuning fork for microsensing applications in high-temperature and high G-shock environments显示文摘We present the fabrication and testing of a silicon carbide(SiC)balanced mass double-ended tuning fork that survives harsh environments without compromising the device strain sensitivity and resolution bandwidth.The device features a material stack that survives corrosive environments and enables high-temperature operation.To perform high-temperature testing,a specialized setup was constructed that allows the tuning fork to be characterized using traditional silicon electronics.The tuning fork has been operated at 600 ℃ in the presence of dry steam for short durations.This tuning fork has also been tested to 64 000 G using a hard-launch,soft-catch shock implemented with a light gas gun.However,the device still has a strain sensitivity of 66 Hz/με and strain resolution of 0.045 με in a 10 kHz bandwidth.As such,this balanced-mass double-ended tuning fork can be used to create a variety of different sensors including strain gauges,accelerometers,gyroscopes,and pressure transducers.Given the adaptable fabrication process flow,this device could be useful to micro-electro-mechanical systems(MEMS) designers creating sensors for a variety of different applications. | David R Myers Kan Bun Cheng Babak Jamshidi Robert G Azevedo Debbie G Senesky Li Chen Mehran Mehregany Muthu B J Wijesundara Albert P Pisano | 2012 | Engineering Sciences2012，10，5： | 6 |
| 9 | Identification of ExpIdentification of Expressed Resistance Gene Analogs from Peanut (Arachis hypogaea L.) Expressed Sequence Tags显示文摘Low genetic diversity makes peanut (Arachis hypogaea L.) very vulnerable to plant pathogens, causing severe yield loss and reduced seed quality. Several hundred partial genomic DNA sequences as nucleotide-binding-site leucine-rich repeat (NBS-LRR) resistance genes (R) have been identified, but a small portion with expressed transcripts has been found. We aimed to identify resistance gene analogs (RGAs) from peanut expressed sequence tags (ESTs) and to develop polymorphic markers. The protein sequences of 54 known R genes were used to identify homologs from peanut ESTs from public databases. A total of 1,053 ESTs corresponding to six different classes of known R genes were recovered, and assembled 156 contigs and 229 singletons as peanut-expressed RGAs. There were 69 that encoded for NBS-LRR proteins, 191 that encoded for protein kinases, 82 that encoded for LRR-PK/transmembrane proteins, 28 that encoded for Toxin reductases, 11 that encoded for LRR-domain containing proteins and four that encoded for TM-domain containing proteins. Twenty-eight simple sequence repeats (SSRs) were identified from 25 peanut expressed RGAs. One SSR polymorphic marker (RGA121) was identified. Two polymerase chain reaction-based markers (Ahsw-1 and Ahsw-2) developed from RGA013 were homologous to the Tomato Spotted Wilt Virus (TSWV) resistance gene. All three markers were mapped on the same linkage group AhIV. These expressed RGAs are the source for RGA-tagged marker development and identification of peanut resistance genes. | Zhanji Liu Suping Feng Manish K. Pandey Xiaoping Chen Albert K. Culbreath Rajeev K. Varshney Baozhu Guo | 2013 | Journal of Integrative Plant Biology2013，55，5： | 4 |
| 10 | The Hemodynamic and Adrenergic Effects of Perioperative Dexmedetomidine Infusion after Vascular Surgery显示文摘 | Pekka Talke Richard Chen Brian Thomas Anil Aggarwall Alexandru Gottlieb Per Thorborg Stephen Heard Albert Cheung Stanley Lee Son Antero Kallio | 2000 | Anesthesia & Analgesia2000，，4： | 3 |
| 11 | Effects of Batroxobin on Spatial Learning and Memory Disorder of Rats with Temporal Ischemia and the Expression of HSP32 and HSP70显示文摘 The effect of Batroxobin on spatial memory disorder of left temporal ischemic rats and the expression of HSP32 and HSP70 were investigated with Morri`s water maze and immunohistochemistry methods. The results showed that the mean reaction time and distance of temporal ischemic rats in searching a goal were significantly longer than those of the sham-operated rats and at the same time HSP32 and HSP70 expression of left temporal ischemic region in rats was significantly increased as compared with the sham-operated rats. However, the mean reaction time and distance of the Batroxobin-treated rats were shorter and they used normal strategies more often and earlier than those of ischemic rats. The number of HSP32 and HSP70 immune reactive cells of Batroxobin-treated rats was also less than that of the ischemic group. In conclusion, Batroxobin can improve spatial memory disorder of temporal ischemic rats; and the down-regulation of the expression of HSP32 and HSP70 is probably related to the attenuation of ischemic injury. | 吴卫平 匡培根 姜树军 张小澍 杨炯炯 隋南 Albert Chen 匡培梓 | 2000 | Journal of Traditional Chinese Medicine2000，20，4： | 3 |
| 12 | 采用美国国家癌症数据库数据分析淋巴浸润性微乳头状乳腺癌的预后显示文摘背景与目的浸润性微乳头状癌(invasive micropapillary carcinoma,IMPC)是乳腺癌中一种罕见的亚型。已有研究表明,与浸润性导管癌(invasive ductal carcinoma,IDC)相比,该亚型更易发生淋巴结转移。本研究旨在明确美国国家癌症数据库(National Cancer Database,NCDB)中记录的乳腺IMPC患者的临床特征、结局和淋巴结转移倾向性。方法从NCDB中检索2004–2014年之间诊断为IMPC患者的病历,并对其进行log-rank检验以评估临床特征与总生存期(overall survival,OS)的相关性。采用Cox比例风险模型确定OS相关因素。结果本研究共纳入2660例IMPC患者。5年OS率为87.5%,24.9%患者出现淋巴结转移。阳性淋巴结数≥4的患者的OS短于阴性淋巴结的患者,而阳性淋巴结数为1–3的患者的OS与阴性淋巴结患者相似。年龄<65岁、接受放疗和雌激素受体阳性也与OS延长有关。放疗仅对接受肿块切除术的IMPC患者有效,对进行乳房切除术的患者无效(无论淋巴结阳性/阴性)。结论IMPC患者的有利预后因素包括年龄<65岁、阳性淋巴结数<4、接受放疗和雌激素受体阳性。本研究结果表明,IMPC患者的生存获益与放疗相关,但可能仅限于经肿块切除术治疗的患者。 | Gary DLewis Yan Xing Waqar Haque Tejal Patel Mary Schwartz Albert Chen Andrew Farach Sandra SHatch EBrian Butler Jenny Chang Bin S.Teh | 2020 | 癌症2020，39，2： | 2 |
| 13 | Cep57, a NEDDl-binding pericentriolar material component, is essential for spindle pole integrity显示文摘 | Qixi Wu Runsheng He Haining Zhou Albert CH Yu Bo Zhang Junlin Teng Jianguo Chen | 2012 | Cell Research2012，22，9： | 2 |
| 14 | Phenylketonuria in Hong Kong Chinese: a call for hyperphenylalaninemia newborn screening in the Special Administrative Region, China显示文摘Hyperphenylalaninemia 是在 15 000 实时出生影响约 1 的新陈代谢的最普通的先天的错误之一。在汉语之中, BH4 缺乏导致 hyperphenylalaninemia 比在白种人普通得多。准确诊断为治疗并且基因建议是重要的。在 2000,为苯酮尿的新生的屏蔽由在在整个整个国家的中国的法律是强制的。然而,它还没在香港特别行政区的新生的屏蔽节目被包括,中国。在香港汉语之中的 hyperphenylalaninemia 上的出版数据大部分正在缺乏。我们与严重 6-pyruvoyl-tetrahydropterin synthase (PTPS ) 报导一个 1 岁的香港中国女孩缺乏。病人与婴儿的 hypotonia 介绍了并且作为服的麻痹被误诊。她有的很温和的 hyperphenylalaninemia (95 mol/L ) ,显著地高的 phenylalnine-to-tyrosine 比率(3.1 ) ,和 1109 mlU/L 的提高的催乳激素。基因分析证实了同型结合的已知的引起疾病的变化磅 NM_000317.1:c.259CT;NP_000308.1:p。在 proband 的 P87S。根据我们的本地经验,当 PTPS 缺乏的 hyperphenylalaninemia 线索的估计的流行被报导在 29 542 实时出生,不是本氨基丙酸 hydroxylase 的一个单个案例是 1 时,缺乏被报导了。而且,有在社区以及在医药专业人员之中的继承新陈代谢的疾病的了解的一般缺乏。很经常,临床的怀疑的一个低索引将在诊断,多重不必要、昂贵的调查,延长病态和焦虑导致延期到影响的家庭。我们强烈建议为 hyperphenylalaninemia 屏蔽的那个扩展新生儿应该为在香港特别行政区出生的每个婴儿被实现,中国。 | Chloe Miu Mak Chun-hung Ko Ching-wan Lam Wai-ling Lau Wai-kwan Siu Sammy Pak-lam Chen Chun-yiu Law Chi-kong Lai Chak-man Yu Albert Yan-wo Chan | 2011 | Chinese Medical Journal2011，，16： | 2 |
| 15 | Conformal Finite-Difference time-domain (FDTD) with overlapping grids显示文摘 | K S Yee Jei Shuan Chen and Albert H Chang | 1992 | IEEE Transactions on Antennas and Propagation1992，40，9： | 1 |
| 16 | Microsomal glutathione S-transferase gene polymorphisms and colorectal cancer risk in a Han Chinese population显示文摘 | Hao Zhang Ling-Hong Liao Shuk-Ming Liu Kwok-Wai Lau Albert Kai-Cheong Lai Jin-Hui Zhang Qi Wang Xiao-Qian Chen Wei Wei Hua Liu Jian-Hua Cai Maria Li Lung Susan S. W. Tai Madeline Wu | 2007 | International Journal of Colorectal Disease2007，，10： | 1 |
| 17 | Identification of Expressed Resistance Gene Analogs from Peanut ( Arachis hypogaea L.) Expressed Sequence Tags显示文摘 | Zhanji Liu Suping Feng Manish K. Pandey Xiaoping Chen Albert K. Culbreath Rajeev K. Varshney Baozhu Guo | 2013 | J Integr Plant Biol2013，，5： | 1 |
| 18 | Effect of renal dysfunction in dogs on the disposition and marrow toxicity of melphalan 显示文摘 | Alberts DS Chen HG Benz D | 1981 | BrJ Cancer1981，43，： | 1 |
| 19 | Mevinolin:a highly potent competitive inhibitor of hydroxymethylglutaryl-coenzyme a reductase and a cholesterol-lowering agent显示文摘 | ALBERTS A W CHEN J KURON G | 1980 | Proc Natl Acad Sci USA1980，77，7： | 1 |
| 20 | Cytoglobin Up-regulated by Hydrogen Peroxide Plays a Protective Role in Oxidative Stress显示文摘 | Dan Li Xiao Qian Chen Wu-Jian Li Yu-Hui Yang Jian-Zhi Wang Albert Cheung Hoi Yu | 2007 | Neurochemical Research2007，，8： | 1 |
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