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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Preliminary results of Thymosin-a1 versus interferon-α treatment in patients with HBeAg negative and serum HBV DNA positive chronic hepatitis B显示文摘INTRODUCTIONIn China ,the incidence and mortality of gastric cancer rank the second among all cancers. Recent development of cancer [1-20].The aim of this study was investigat the insight of apoptosis and bcl-2, p53 and C-myc protein expression in the development of gastric cancer . | Lin Zhuang Jing You Bao Zhang Tang Su Ying Ding Kui Hua Yan Dan Peng Yan Mei Zhang Lu Zhang ~1Department of Hepatology,Kunming Third Municipal Peoples Hospital,Kunming 650041,Yunnan Province,China ~2Department of Infectious Diseases,The First Affiliated Hospital of Kunming Medical College,Kunming 650032,Yunnan Province,China | 2001 | World Journal of Gastroenterology2001，7，3： | 22 |
| 3 | Review of the characteristics and graded utilisation of coal gasification slag显示文摘The characteristics of the energy structure of rich coal,less oil and less gas,coupling with a high external dependence on oil and natural gas and the emphasis on the efficient and clean utilisation of coal,have brought opportunities for coal chemical industry.However,with the large-scale popularisation of coal gasification technology,the production and resulting storage of coal gasification slag continue to increase,which not only result in serious environmental pollution and a waste of terrestrial resources,but also seriously affect the sustainable development of coal chemical enterprises.Hence,the treatment of coal gasification slag is extremely important.In this paper,the production,composition,morphology,particle size structure and water holding characteristics of coal gasification slag are introduced,and the methods of carbon ash separation of gasification slag,both domestically and abroad,are summarised.In addition,the paper also summarises the research progress on gasification slag in building materials,ecological restoration,residual carbon utilisation and other high-value utilisation,and ultimately puts forward the idea of the comprehensive utilisation of gasification slag.For large-scale consumption to solve the environmental problems of enterprises and achieve high-value utilisation to increase the economic benefits of enterprises,it is urgent to zealously design a reasonable and comprehensive utilisation technologies with simple operational processes,strong adaptability and economic benefits. | Xiaodong Liu Zhengwei Jin Yunhuan Jing Panpan Fan Zhili Qi Weiren Bao Jiancheng Wang Xiaohui Yan Peng Lv Lianping Dong | 2021 | Chinese Journal of Chemical Engineering2021，34，7： | 21 |
| 4 | Achieving partial nitrification by inhibiting the activity of Nitrospira-like bacteria under high-DO conditions in an intermittent aeration reactor显示文摘It is generally accepted that a low dissolved oxygen(DO) concentration is more beneficial for achieving partial nitrification than high-DO. In this study, partial nitrification was not established under low-DO conditions in an intermittent aeration reactor for treating domestic wastewater. During the operational period of low-DO conditions(DO: 0.3 ±0.14 mg/L), stable complete nitrification was observed. The abundance of Nitrospira-like bacteria, which were the major nitrite-oxidizing bacteria, increased from 1.03 × 10~6to2.64 × 10~6cells/m L. At the end of the low-DO period, the batch tests showed that high-DO concentration(1.5, 2.0 mg/L) could inhibit nitrite oxidation, and enhance ammonia oxidation. After switching to the high-DO period(1.8 ± 0.32 mg/L), partial nitrification was gradually achieved. Nitrospira decreased from 2.64 × 10~6 to 8.85 × 10~5cells/m L. It was found that suddenly switching to a high-DO condition could inhibit the activity and abundance of Nitrospira-like bacteria, resulting in partial nitrification. | Peng Bao Shuying Wang Bin Ma Qiong Zhang Yongzhen Peng | 2017 | Journal of Environmental Sciences2017，29，6： | 20 |
| 5 | OsVPS9A Functions Cooperatively with OsRAB5A to Regulate Post-Golgi Dense Vesicle-Mediated Storage Protein Trafficking to the Protein Storage Vacuole in Rice Endosperm Cells显示文摘在米饭内乳房间, glutelins 作为 proglutelins 在不平的 endoplasmic 蜂窝胃上被综合并且被排序到称为蛋白质身体 II (PBII ) 的 theprotein 存储液泡(PSV ) ,在他们被变换成成熟形式的地方。稠密的泡(DV ) 调停了在大米种子的 proglutelins 的 trafficking 被建议了,但是这个过程的 post-Golgi 控制是 largelyunknown。DV 是否能与 PSV 直接熔化,是争论的另一件事。在这研究,我们建议规章的 mechanismunderlying 调停 DV, post-Golgi proglutelin trafficking 到 PBII (PSV ) 。gpa2, OsVPS9A 的 loss-of-function 异种,编码 OsRAB5A 的 GEF,积累了 uncleaved proglutelins。Proglutelins 对到沿着在 DV 形式的房间墙的 apoplast 的 paramural bodiesand 指向 mis,它在 PBII 尺寸导致了伴随物减小。Previouslyreported gpa1,在 OsRab5a 变异,有类似的显型,当时两倍异种加重了的 gpa1gpa2 条件。另外, OsVPS9A 在 vitro 并且在 vivo 与 OsRAB5A 交往了。我们断定 OsVPS9A 和 OsRAB5A 可以一起工作并且在调停 DV 的 post-Golgi proglutelintrafficking 起一个规章的作用到 PBII (PSV ) 。DV 可能直接熔化到 PBII (PSV ) 交付货物的证据也是 presented.Key 词: | Feng Liu Yulong Ren Yihua Wang Cheng Peng Kunneng Zhou Jia Lv Xiuping Guo Xin Zhang Jianmin wan Yiqun Bao | 2013 | Molecular Plant2013，6，6： | 16 |
| 6 | Recurrent gain-of-function USP8 mutations in Cushing's disease显示文摘库欣的疾病,引起过量皮质醇生产的也已知的同样促肾上腺皮质的荷尔蒙(ACTH )-secreting 垂体腺瘤(舞步) ,占多达 85%corticotrophin 依赖的库欣的症候群盒子。然而,在这疾病的基因改变是不清楚的。这里,我们执行了从 12 秘密 ACTH 舞步导出并且匹配血样品的 DNA 定序的 whole-exome,它在候选人基因揭示了体的变化的三种类型, USP8 (编码 ubiquitin 特定的朊酶 8 ) ,专门在 exon 14 在 12 秘密 ACTH 舞步中的 8 个。我们进一步评估了体的 USP8 变化在另外由定序的 Sanger 的 258 舞步。进一步定序指向在 108 秘密 ACTH 舞步(62.04%) 中的 67 个识别了 USP8 变体的 17 种类型的一个总数。然而,任何一个都没在舞步的另外的类型这些变化被检测(n = 150 ) 。这些变化在 USP8 的 14-3-3 绑定主题以内聚集并且破坏在 USP8 和 14-3-3 蛋白质之间的相互作用,导致保护 EGFR 免受 lysosomal 降级的伤害的一个提高的能力。因此,有 EGFR 表示,提高的 EGFR 蛋白质丰富和 mRNA 表示的更高的发生 POMC 铺平的变异的 USP8 显示的舞步,它编码 ACTH 的先锋。有变异的 USP8 的舞步在尺寸是显著地更小的并且比野类型的舞步有更高的 ACTH 生产。在里面通过手术 resected 主要变异 USP8 的肿瘤房间, USP8 击倒或堵住的 EGFR 有效地稀释 ACTH 分泌物。一起拿,体的 gain-of-function USP8 变化是普通的并且在库欣的疾病贡献 ACTH 生产过剩。USP8 或 EGFR 的抑制为对待变异 USP8 的 corticotrophin 腺瘤是有希望的。我们的学习在库欣的疾病加亮潜在地功能的变异的基因并且提供卓见进这疾病的治疗学。 | Zeng-Yi Ma Zhi-Jian Song Jian-Hua Chen Yong-Fei Wang Shi-Qi Li Liang-Fu Zhou Ying Mao Yi-Ming Li Rong-Gui Hu Zhao-Yun Zhang Hong-Ying Ye Ming Shen Xue-Fei Shou Zhi-Qiang Li Hong Peng Qing-Zhong Wang Dai-Zhan Zhou Xiao-Lan Qin Jue Ji Jie Zheng Hong Chen Yin Wang Dao-Ying Geng Wei-Jun Tang Chao-Wei Fu Zhi-Feng Shi Yi-Chao Zhang Zhao Ye Wen-Qiang He Qi-Lin Zhang Qi-Sheng Tang Rong Xie Jia-Wei Shen Zu-Jia Wen Juan Zhou Tao Wang Shan Huang Hui-Jia Qiu Ni-Dan Qiao Yi Zhang Li Pan Wei-Min Bao Ying-Chao Liu Chuan-Xin Huang Yong-Yong Shi Yao Zhao | 2015 | Cell Research2015，25，3： | 16 |
| 7 | Integrated method of guidance,control and morphing for hypersonic morphing vehicle in glide phase显示文摘The morphing technology of hypersonic vehicle improved the flight performance by changing aerodynamic characteristics with shape deformations,but the design of guidance and control system with morphing laws remained to be explored.An Integrated of Guidance,Control and Morphing(IGCM)method for Hypersonic Morphing Vehicle(HMV)was developed in this paper.The IGCM method contributed to an effective solution of morphing characteristic to improve flight performance and reject the disturbance for guidance and control system caused by the morphing system for HMV in gliding phase.The IGCM models were established based on the motion models and aerodynamic models of the variable span vehicle.Then the IGCM method was designed by adaptive block dynamic surface back-stepping method with stability proof.The parallel controlled simulations’results showed the effectiveness in accomplishing the flight mission of IGCM method in glide phase with smaller terminal errors.The velocity loss of HMV was reduced by 32.8%which inferred less flight time and larger terminal flight velocity than invariable span vehicle.Under the condition of large deviations of aerodynamic parameters and atmospheric density,the robustness of IGCM method with variable span was verified. | Cunyu BAO Peng WANG Guojian | 2021 | Chinese Journal of Aeronautics2021，34，5： | 16 |
| 8 | A new triterpene glycoside from sea cucumber Holothuria leucospilota显示文摘A new triterpene glycoside, leucospilotaside A, along with a known saponin, isolated from sea cucumber Holothuria leucospilota, and its structure was elucidated as 3β-O-[4-O-sodiumsulfate-β-D-quinovopyranosyl-(1→2)-β-D-xylopyranosyl]-holosta-22-ketone-9-en-17α,25α-diol (1) by extensive spectroscopic analysis and chemical methods. Leucospilotaside A (1) has a ketone carbonyl group (22) in the aglycon side chain. | Hua Han Yang Hua Yi Ling Li Xiao Hua Wang Bao Shu Liu Peng Sun Min Xiang Pan | 2007 | Chinese Chemical Letters2007，18，2： | 13 |
| 9 | Serum uric acid levels are associated with polymorphisms in the SLC2A9, SF1, and GCKR genes in a Chinese population显示文摘 | Xue SUN Feng JIANG Rong ZHANG Shan-shan TANG Miao CHEN Dan-feng PENG Jing YAN Tao WANG Shi-yun WANG Yu-qian BAO Cheng HU Wei-ping JIA | 2014 | Acta Pharmacologica Sinica2014，35，11： | 12 |
| 10 | Harpin_(xoo) and Its Functional Domains Activate Pathogen-inducible Plant Promoters in Arabidopsis显示文摘Harpins are bacterial proteins that can enhance plant growth and defense against pathogens and insects. To elaborate whether harpins perform the diverse functions in coordination with the activation of specific promoters that contain particular elements, we cloned pathogen-inducible plant promoters PPP1, PPP2, and PPP3 from tobacco and investigated their responses to harpinxoo or its truncated fragments DEG, DIR, and DPR (domains for enhancing plant growth, insect resistance and pathogen resistance). PPP1 contains an internal repeat composed of two tandem 111bp fragments; 111bp in the repeat was deleted in PPP2. PPP3 contains a bacteria-inducible element; PPP1 and PPP2 additionally contain TAC-1 and Eli boxes inducible correspondingly by salicylic acid (SA) and elicitors. Function of cloned PPPs was confirmed based on their activation in transgenic Arabidopsis plants by Ralstonia solanacearum (Ralston) or SA. Harpinxoo, DEG, DIR, or DPR activated PPP1 and PPP2 but not PPP3, consistent with the presence of Eli boxes in promoters. PPP1 was ca. 3-fold more active than PPP2, suggesting that the internal repeat affects levels of the promoter activation. | PENG Jian-Ling BAO Zhi-Long LI Ping CHEN Guang-Yong WANG Jin-Sheng DONG Han-Song | 2004 | Acta Botanica Sinica2004，46，9： | 12 |
| 11 | Orthogonal Test Design for Optimization of the Extraction of Flavonid from the Fructus Gardeniae显示文摘Objective It is imperative to provide some consistent experimental results for the extraction of flavonid from Fructus Gardeniae.Methods The key extraction parameters that influenced the yield of flavonid from Fructus Gardeniae were optimized by employing an orthogonal experiment [L9(3)4],including the ratio of buffer solution (Na2B4O7·10H2O) to raw material,concentration of Fructus Gardeniae in extracting solution,extraction time and pH of buffer solution.An UV/Vis detector was used to perform the qualitative and quantitative analyses of the extracted flavonid with the using of the standard sample.Results The maximum extraction yield of the crude extract was 5.0533 (mg/g) after 20 min when the mass ratio of Na2B4O7·10H2O to raw material was 0.4%,the concentration of Fructus Gardeniae in the extraction solution was 1/12 (g/mL),and pH of buffer solution was 4.5.The positive reactions to the Molish and HCl‐Mg tests suggested that the extracted compound was flavonoid,and FTIR measurements also identified the presence of flavonoid in the extracts.Conclusion This work is expected to provide a basis for further research,development,and utilization of Fructus gardenia in flavonid extraction. | CAI Ding Jian SHU Qing XU Bao Quan PENG Li Mei HE Yan | 2011 | Biomedical and Environmental Sciences2011，24，6： | 12 |
| 12 | Effect of main inclusions on crack initiation in bearing steel in the very high cycle fatigue regime显示文摘This work aims to investigate the effect of main inclusions on crack initiation in bearing steel in the very high cycle fatigue(VHCF) regime. The size and type of inclusions in the steel were quantitatively analyzed, and VHCF tests were performed. Some fatigue cracks were found to be initiated in the gaps between inclusions(Al2 O3, Mg O-Al2 O3) and the matrix, while other cracks originated from the interior of inclusions(Ti N, Mn S). To explain the related mechanism, the tessellated stresses between inclusions and the matrix were calculated and compared with the yield stress of the matrix. Results revealed that the inclusions could be classified into two types under VHCF; of these two, only one type could be regarded as holes. Findings in this research provide a better understanding of how inclusions affect the high cycle fatigue properties of bearing steel. | Chao Gu Yan-ping Bao Peng Gan Min Wang Jin-shan He | 2018 | International Journal of Minerals,Metallurgy and Materials2018，25，6： | 11 |
| 13 | Re–Os dating of molybdenite and in-situ Pb isotopes of sulfides from the Lamo Zn–Cu deposit in the Dachang tin-polymetallic ore field, Guangxi, China显示文摘The Dachang tin-polymetallic district, Guangxi,China, is one of the largest tin ore fields in the world. Both cassiterite-sulfide and Zn–Cu skarn mineralization are hosted in the Mid-Upper Devonian carbonate-rich sediments adjacent to the underlying Cretaceous Longxianggai granite(91–97 Ma). The Lamo Zn–Cu deposit is a typical skarn deposit in the district and occurs at the contact zone between the Upper Devonian limestone and the granite.The ore minerals mainly consist of sphalerite, arsenopyrite,pyrrhotite, galena, chalcopyrite, and minor molybdenite.However, the age of mineralization and source of the metals are not well constrained. In this study, we use the molybdenite Re–Os dating method and in-situ Pb isotopes of sulfides from the Lamo deposit for the first time in order to directly determine the age of mineralization and the tracing source of metals. Six molybdenite samples yielded a more accurate Re–Os isochron age of 90.0 ± 1.1 Ma(MSWD = 0.72), which is much younger than the reported garnet Sm–Nd isochron age of 95 ± 11 Ma and quartz fluid inclusions Rb–Sr isochron age of 99 ± 6 Ma. This age is also interpreted as the age of Zn–Cu skarn mineralization in the Dachang district. Further, in this study we found that in-situ Pb isotopes of sulfides from the Lamo deposit and feldspars in the district's biotite granite and granitic porphyry dikes have a narrow range and an overlap of Pb isotopic compositions(^(206) Pb/^(204) Pb =18.417–18.594,^(207) Pb/^(204) Pb = 15.641–15.746, and^(208) Pb/^(204) Pb = 38.791–39.073), suggesting that the metals were mainly sourced from Cretaceous granitic magma. | Hai Zhao Wenchao Su Peng Xie Nengping Shen Jiali Cai Ming Luo Jie Li Zhian Bao | 2018 | Acta Geochimica2018，37，3： | 11 |
| 14 | Total mesopancreas excision for pancreatic head cancer: analysis of 120 cases显示文摘Objective: To evaluate the feasibility and safety of total mesopancreas excision(TMpE) in the treatment of pancreatic head cancer. Methods: The clinical and pathological data of 120 patients with pancreatic head cancer who had undergone TMpE in our center from May 2010 to January 2014 were retrospectively analyzed. Results: The mean operative time was(275.0±50.2) min and the average intra-operative blood loss was(390.0±160.5) mL. Post-operative complications were reported in 45 patients, while no peri-operative death was noted. The specimen margins were measured in three dimensions, and 86 patients(71.6%) achieved R0 resection. Conclusions: TMpE is safe and feasible for pancreatic head cancer and is particularly helpful to increase the R0 resection rate. | Wenguang Wu Xu'an Wang Xiangsong Wu Maolan Li Hao Weng Yang Cao Ruifa Bao Sijun Su Jianhua Lu Wei Gong Weibin Shi Jun Gu Xuefeng Wang Yingbin Liu Zhiwei Quan Shuyou Peng | 2016 | Chinese Journal of Cancer Research2016，28，4： | 10 |
| 15 | Weighted Markov chains for forecasting and analysis in Incidence of infectious diseases in jiangsu Province,China显示文摘This paper first applies the sequential cluster method to set up the classification standard of infectious disease incidence state based on the fact that there are many uncertainty characteristics in the incidence course.Then the paper presents a weighted Markov chain,a method which is used to predict the future incidence state.This method assumes the standardized self-coefficients as weights based on the special characteristics of infectious disease incidence being a dependent stochastic variable.It also analyzes the characteristics of infectious diseases incidence via the Markov chain Monte Carlo method to make the long-term benefit of decision optimal.Our method is successfully validated using existing incidents data of infectious diseases in Jiangsu Province.In summation,this paper proposes ways to improve the accuracy of the weighted Markov chain,specifically in the field of infection epidemiology. | Zhihang Peng Changjun Bao Yang Zhao Honggang Yi Letian Xia Hao Yu Hongbing Shen Feng Chen | 2010 | The Journal of Biomedical Research2010，24，3： | 10 |
| 16 | Analysis of crack propagation in concrete structures with structural information entropy显示文摘The propagation of cracks in concrete structures causes energy dissipation and release, and also causes energy redistribution in the structures. Entropy can characterize the energy redistribution. To investigate the relation between the propagation of cracks and the entropy in concrete structures, cracked concrete structures are treated as dissipative structures. Structural information entropy is defined for concrete structures. A compact tension test is conducted. Meanwhile, numerical simulations are also carried out. Both the test and numerical simulation results show that the structural information entropy in the structures can characterize the propagation of cracks in concrete structures. | BAO TengFei1’2, PENG Yan1’2, CONG PeiJiang1’2 & WANG JiaLin1’2 1 State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Hohai University, Nanjing 210024, China 2 National Engineering Research Center ofWater Resources Efficient Utilization and Engineering Safety, Hohai University, Nanjing 210024, China | 2010 | Science China(Technological Sciences)2010，53，7： | 9 |
| 17 | Serum soluble ST2 is a promising prognostic biomarker in HBV-related acute-on-chronic liver failure显示文摘BACKGROUND: The IL-33/ST2 axis is involved in the pathogenesis of many diseases such as autoimmune diseases, cancer,and heart failure. However, studies of the IL-33/ST2 pathway in HBV-related acute-on-chronic liver failure(HBV-ACLF) are lacking. The present study aimed to determine the prognostic role of serum IL-33/soluble ST2(s ST2) in HBV-ACLF.METHODS: Serum levels of IL-33 and sS T2 in healthy controls(HC, n=18), chronic hepatitis B(CHB, n=27) and HBV-ACLF(n=51) patients at the 1st and 4th week after enrollment were detected using ELISA, and clinical data were collected. The follow-up of HBV-ACLF patients lasted for 6 months at least.RESULTS: There was no significant difference of serum IL-33 level among HC, CHB and HBV-ACLF patients at week 1.However, serum s ST2 level differed significantly among the three groups: highest in the HBV-ACLF group, moderate in the CHB group and lowest in the HC group. There was a reverse correlation between serum s ST2 level and the survival of HBV-ACLF patients. The level of serum s ST2 in HBV-ACLF survivors was significantly declined from week 1 to week 4 following the treatment, whereas that in HBV-ACLF nonsurvivors remained at a high level during the same period. Furthermore, serum sS T2 level was significantly correlated with laboratory parameters and the most updated prognostic scores(CLIF-C OF score, CLIF-C ACLF score and ACLF grades). Thereceiver operating characteristics curves demonstrated that serum sS T2 level was a good diagnostic marker for predicting the 6-month mortality in HBV-ACLF patients, comparable to the most updated prognostic scores. Serum sS T2 cut-off points for predicting prognosis in HBV-ACLF patients were 76 ng/mL at week 1 or 53 ng/mL at week 4, respectively. HBV-ACLF patients with serum sS T2 level above the cut-off point often had a worse prognosis than those below the cut-off point.CONCLUSION: Serum s ST2 may act as a promising biomarker to assess severity and predict prognosis of patients with HBV-ACLF and help for the early identification and optimal treatment of HBV-ACLF patients at high risk of mortality. | Shao-Wen Jiang Peng Wang Xiao-Gang Xiang Rui-Dong Mo Lan-Yi Lin Shi-San Bao Jie Lu Qing Xie | 2017 | Hepatobiliary & Pancreatic Diseases International2017，16，2： | 9 |
| 18 | Osteogenic differentiation of mesenchymal stem cells promoted by overexpression of connective tissue growth factor显示文摘Objective:Large segmental bone defect repair remains a clinical and scientific challenge with increasing interest focusing on combining gene transfection with tissue engineering techniques.The aim of this study is to investigate the effect of connective tissue growth factor(CTGF) on the proliferation and osteogenic differentiation of the bone marrow mesenchymal stem cells(MSCs).Methods:A CTGF-expressing plasmid(pCTGF) was constructed and transfected into MSCs.Then expressions of bone morphogenesis-related genes,proliferation rate,alkaline phosphatase activity,and mineralization were examined to evaluate the osteogenic potential of the CTGF gene-modified MSCs.Results:Overexpression of CTGF was confirmed in pCTGF-MSCs.pCTGF transfection significantly enhanced the proliferation rates of pCTGF-MSCs(P<0.05).CTGF induced a 7.5-fold increase in cell migration over control(P<0.05).pCTGF transfection enhanced the expression of bone matrix proteins,such as bone sialo-protein,osteocalcin,and collagen type I in MSCs.The levels of alkaline phosphatase(ALP) activities of pCTGF-MSCs at the 1st and 2nd weeks were 4.0-and 3.0-fold higher than those of MSCs cultured in OS-medium,significantly higher than those of mock-MSCs and normal control MSCs(P<0.05).Overexpression of CTGF in MSCs enhanced the capability to form mineralized nodules.Conclusion:Overexpression of CTGF could improve the osteogenic differentiation ability of MSCs,and the CTGF gene-modified MSCs are potential as novel cell resources of bone tissue engineering. | Jin-jing WANG Feng YE Li-jia CHENG Yu-jun SHI Ji BAO Huai-qiang SUN Wei WANG Peng ZHANG Hong BU | 2009 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2009，10，5： | 8 |
| 19 | Cross-correlation matrix Root-MUSIC algorithm for bistatic multiple-input multiple-output radar显示文摘A novel Root-MUSIC algorithm based on cross-correlation matrix is proposed to estimate the direction of arrival(DOA) and direction of departure(DOD) for the bistatic multiple-input multiple-output(MIMO)radar. In the algorithm, the cross-correlation matrix is first constructed by using the received data which are arranged according to the transmit array and the receive array, respectively. And the DOAs and DODs are estimated independently employing cross-correlation Root-MUSIC algorithms without eigendecomposition,where the null spaces are constructed with a linear operation of the cross-correlation matrices. Secondly the pair-matching of DOAs and DODs is accomplished by solving an angle estimate optimization. The effectiveness of the proposed method is verified through numerical simulations, and it is shown that the proposed method can provide more accurate angle estimation with small computational complexity than conventional eigendecomposition algorithms. | QIN GuoDong BAO Dan LIU GaoGao LI Peng | 2015 | Science China(Information Sciences)2015，58，2： | 8 |
| 20 | Association between Plasma Metal Levels and Diabetes Risk: a Case-control Study in China显示文摘Objective Many metals, some of which have been classified as environmental endocrine disruptors, are used extensively in everyday consumer products and are ubiquitous in our living environment. In the present study, we aimed to explore the associations between the prevalence risk of type 2 diabetes and plasma levels of 20 trace elements as well as those of heavy metals in a Han Chinese population. Methods We conducted a case-control study to investigate the associations between plasma concentrations of 20 metals and diabetes in Jiangsu province. A total of 122 newly diagnosed cases of type 2 diabetes and 429 matched controls were recruited from community physical examinations in Suzhou City of Jiangsu Province. Plasma metal levels were measured by inductively-coupled plasma mass spectrometry. Results After adjusting for confounders, plasma vanadium, chromium, manganese, copper, zinc, arsenic, selenium, strontium, palladium, cadmium, cesium, and barium were associated with diabetes risk(P < 0.05). The adjusted OR increased with increasing concentration of vanadium, manganese, copper, zinc, and cesium. Conclusion Many metals, including manganese, copper, zinc, arsenic, selenium, and cadmium in plasma, are associated with the morbidity of diabetes. Monitoring of environmental metal levels and further studies are urgently needed. | LI Xiu Ting YU Peng Fei GAO Yan GUO Wen Hui WANG Jun LIU Xin GU Ai Hua JI Gui Xiang DONG Qiu WANG Bo Shen CAO Ying ZHU Bao Li XIAO Hang | 2017 | Biomedical and Environmental Sciences2017，30，7： | 8 |
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