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| 1 | A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants显示文摘CRISPR/Cas9 染色体指向系统被用于许多种类。然而,为植物报导的 mostCRISPR/Cas9 系统能仅仅修改一个或一些指向地点。这里,我们报导一个柔韧的 CRISPR/Cas9 向量系统,利用植物 codon 优化了 Cas9 基因,为方便、高效率的复合染色体在 monocot 和 dicot 植物编辑。我们设计了基于 PCR 的过程很快产生多重 sgRNA 表达式盒子,它能在一个轮由金门结扎或吉布森汇编克隆被装配进二进制 CRISPR/Cas9 向量。与这个系统,我们与 85.4% 变化评估的一般水准在米饭编辑了 46 个目标地点,主要在 biallelic 和同型结合的地位。我们推想在米饭的大约 16% 同型结合的变化通过相应基于再结合的修理跟随的非相应的加入结束的机制被产生。我们也获得了一致 biallelic,在 Arabidopsis T 1 植物的异质接合、同型结合、妄想的变化。在米饭和 Arabidopsis 的指向的变化是可继承的。我们在 T 0 米饭和 T 1 由同时的指向的 Arabidopsis 植物多重(多达八) 一个基因家庭,在一条 biosynthetic 小径的多重基因,或在单个基因的多重地点的成员。这个系统为为基本研究和基因改进在植物学习多重基因和基因家庭的功能提供了一个万用的工具箱。 | Xingliang Ma Qunyu Zhang Qinlong Zhu Wei Liu Yan Chen Rong Qiu Bin Wang Zhongfang Yang Heying Li Yuru Lin Yongyao Xie Rongxin Shen Shuifu Chen Zhi Wang Yuanling Chen Jingxin Guo Letian Chen Xiucai Zhao Zhicheng Dong Yao-Guang Liu | 2015 | Molecular Plant2015，8，8： | 272 |
| 2 | Spatiotemporal variations of PM_(2.5) and PM_(10) concentrations between31 Chinese cities and their relationships with SO_2,NO_2,CO and O_3显示文摘The variations of mass concentrations of PM_(2.5),PM_(10),SO_2,NO_2,CO,and O_3 in 31 Chinese provincial capital cities were analyzed based on data from 286 monitoring sites obtained between March 22,2013 and March 31,2014.By comparing the pollutant concentrations over this length of time,the characteristics of the monthly variations of mass concentrations of air pollutants were determined.We used the Pearson correlation coefficient to establish the relationship between PM_(2.5),PM_(10),and the gas pollutants.The results revealed significant differences in the concentration levels of air pollutants and in the variations between the different cities.The Pearson correlation coefficients between PMs and NO_2 and SO_2 were either high or moderate(PM_(2.5) with NO_2:r=0.256-0.688,mean r=0.498;PM_(10) with NO_2:r = 0.169-0.713,mean r = 0.493;PM2.5 with SO_2:r= 0.232-0.693,mean r=0.449;PM_(10) with SO_2:r = 0.131-0.669,mean r=0.403).The correlation between PMs and CO was diverse(PM_(2.5):r = 0.156-0.721,mean r=0.437;PM_(10):r=0.06-0.67,mean r= 0.380).The correlation between PMs and O_3 was either weak or uncorrelated(PM_(2.5):r--0.35 to 0.089,mean r=-0.164;PM_(10):r=-0.279 to 0.078,mean r=-0.127),except in Haikou(PM2.5:r=0.500;PM_(10):r-0.509). | Yangyang Xie Bin Zhao Lin Zhang Rong Luo | 2015 | Particuology2015，13，3： | 56 |
| 3 | Comparison of microRNA profiles of human periodontal diseased and healthy gingival tissues显示文摘MicroRNAs (miRNAs) have been demonstrated to play an important role in regulation of the immuno- inflammatory response; however, the function of miRNAs in periodontal inflammation has not been investigated. The objective of this study was to explore the properties of miRNAs in periodontal inflammation by comparing miRNA profiles of inflamed and healthy gingival tissues. Gingival tissues were obtained from 10 periodontitis patients and 10 healthy subjects. After RNA extraction, miRNA profiles were analyzed by microarray, and expression levels of selected miRNAs were confirmed by real-time quantitative reverse transcription polymerase chain reaction (RT-PCR). Analyses using two computational methods, Targetscan and MicroRNA.org, were combined to identify common targets of these miRNAs. Finally, the individual miRNA expression levels of three toll-like receptor (TLR)-related miRNAs from inflamed and healthy gingival tissues were evaluated by RT-PCR. Ninety-one miRNAs were found to be upregulated and thirty-four downregulated over two-fold in inflamed gingival tissue compared with those in healthy gingival tissue. Twelve selected inflammatory-related miRNAs, hsa-miR-126*, hsa-miR-20a, hsa-miR-142-3p, hsa-miR-19a, hsa-let-7f, hsa-miR-203, hsa-miR-17, hsa-miR-223, hsa-miR-146b, hsa-miR-146a, hsa-miR-155, and hsa-miR-205 showed comparable expression levels by microarray and real-time quantitative RT-PCR analyses. In addition, the putative inflammation targets of these miRNAs were predicted, and three that were tested (hsa-miRNA-146a, hsa-miRNA-146b, and hsa-miRNA-155), showed significant differences between inflamed and healthy gingiva. This remarkable difference in miRNA profiles between periodontal diseased and healthy gingiva implicates a probable close relationship between miRNAs and periodontal inflammation. The data also suggest that the regulation of TLRs in periodontal inflammation may involve miRNA pathways. | Yu-feng Xie Rong Shu Shao-yun Jiang Da-li Liu Xiu-li Zhang | 2011 | International Journal of Oral Science2011，3，3： | 36 |
| 4 | Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis显示文摘 | Yao Chen Yuxuan Zheng Yun Gao Zhen Lin Suming Yang Tongtong Wang Qiu Wang Nannan Xie Rong Hua Mingxi Liu Jiahao Sha Michael D. Griswold Jinsong Li Fuchou Tang Ming-Han Tong | 2018 | Cell Research2018，28，9： | 26 |
| 5 | Exploration of the formation mechanism and source attribution of ambient ozone in Chongqing with an observation-based model显示文摘An intensive field campaign was conducted in Chongqing during the summer of 2015 to explore the formation mechanisms of ozone pollution. The sources of ozone, the local production rates, and the controlling factors, as well as key species of volatile organic compounds(VOCs), were quantified by integrating a local ozone budget analysis, calculations of the relative incremental reactivity, and an empirical kinetic model approach. It was found that the potential for rapid local ozone formation exists in Chongqing. During ozone pollution episodes, the ozone production rates were found to be high at the upwind station Nan Quan, the urban station Chao Zhan, and the downwind station Jin-Yun Shan. The average local ozone production rate was 30×10^(-9) V/V h^(-1) and the daily integration of the produced ozone was greater than 180×10^(-9) V/V. High ozone concentrations were associated with urban and downwind air masses. At most sites, the local ozone production was VOC-limited and the key species were aromatics and alkene, which originated mainly from vehicles and solvent usage. In addition, the air masses at the northwestern rural sites were NO_x-limited and the local ozone production rates were significantly higher during the pollution episodes due to the increased NOx concentrations. In summary, the ozone abatement strategies of Chongqing should be focused on the mitigation of VOCs. Nevertheless, a reduction in NO_x is also beneficial for reducing the regional ozone peak values in Chongqing and the surrounding areas. | SU Rong LU KeDing YU JiaYan TAN ZhaoFeng JIANG MeiQing LI Jing XIE ShaoDong WU YuSheng ZENG LiMin ZHAI ChongZhi ZHANG YuanHang | 2018 | Science China Earth Sciences2018，61，1： | 24 |
| 6 | Motion trajectory prediction based on a CNN-LSTM sequential model显示文摘Accurate monitoring the surrounding environment is an important research direction in the field of unmanned systems such as bio-robotics,and has attracted much research attention in recent years.The trajectories of surrounding vehicles should be predicted accurately in space and time to realize active defense and running safety of an unmanned system.However,there is uncertainty and uncontrollability in the process of trajectory prediction of surrounding obstacles.In this study,we propose a trajectory prediction method based on a sequential model,that fuses two neural networks of a convolutional neural network(CNN)and a long short-term memory network(LSTM).First,a box plot is used to detect and eliminate abnormal values of vehicle trajectories,and valid trajectory data are obtained.Second,the trajectories of surrounding vehicles are predicted by merging the characteristics of CNN space expansion and LSTM time expansion;the hyper-parameters of the model are optimized according to a grid search algorithm,which satisfies the double-precision prediction requirement in space and time.Finally,data from next generation simulation(NGSIM)and Creteil roundabout in France are taken as test cases;the correctness and rationality of the method are verified by prediction error indicators.Experimental results demonstrate that the proposed CNNLSTM method is more accurate and features a shorter time cost,which meets the prediction requirements and provides an effective method for the safe operation of unmanned systems. | Guo XIE Anqi SHANGGUAN Rong FEI Wenjiang JI Weigang MA Xinhong HEI | 2020 | Science China(Information Sciences)2020，63，11： | 24 |
| 7 | Spinal surgeons' learning curve for lumbar microendoscopic discectomy: a prospective study of our first 50 and latest 10 cases显示文摘背景 Microendoscopic discectomy ( MED )是为腰部的圆盘形成疝从外科允许快速的恢复的最低限度地侵略的操作,但是因为大多数外科医生避免它 curve.We 在针的 surgeons'learning curve.Methods 的阶段评估了腰部的 MED 的有效性和安全的长学习,在很少医院里代替了传统的开的外科从2002年6月接待 MED 到2003年2月的五十个病人各被划分成十的按年代先后的组: A-E.The 控制组 F 是十 | RONG Li-min XIE Pei-gen SHI De-hai DONG Jian-wen LIU Bin FENG Feng CAI Dao-zhang | 2008 | Chinese Medical Journal2008，，21： | 23 |
| 8 | Nuclear factor kappa B: A marker of chemotherapy for human stage Ⅳ gastric carcinoma显示文摘AIM: To detect the nuclear factor kappa B (NF-κB) condition in human stage Ⅳ gastric carcinoma patients and to explore the correlation between NF- κB activation and survival of these patients after chemotherapy. METHODS: Expression of NF-κB-p65 was determined by immunohistochemical analysis. Activity of NF- κB DNA-binding in carcinoma tissue was detected by electrophoretic mobility shift assay. Kaplan-Meier survival analysis was performed to show the relation between NF-κB and progression-free survival (PFS) or overall survival (OS) of the patients. RESULTS: The positive expression rate of NF-κB-p65 in 60 gastric cancer tissue samples was 76.7% (46/60). The expression of NF-κB-p65 was reduced in adjacent carcinoma and normal tissue samples. Electrophoretic mobility shift assay (EMSA) analysis showed a strong activation of NF-κB in cancer tissue samples. A survival difference was found in NF- κB-p65 positive and negative patients. NF-κB-p65 expression was negative in cancer tissue samples (n = 14). PFS was 191.40 ± 59.88 d and 152.93 ± 16.99 d, respectively, in patients with positive NF- κB-p65 expression (n = 46) (P = 0.4028). Thesurvival time of patients with negative and positive NF-κB-p65 expression was 425.16 ± 61.61 d and 418.85 ± 42.98 d, respectively (P = 0.7303). Kaplan-Meier analysis showed no significant difference in PFS or OS. The 46 patient tissue which positive NF- κB-p65 expression was found in the tissue samples from the 46 patients whose PFS and OS were 564.89 ± 75.94 d and s 352.37 ± 41.32 d, respectively (P = 0.0165). CONCLUSION: NF-κB is activated in gastric carcinoma tissue, which is related to the OS after chemotherapy. | Sheng Ye You-Ming Long Jian Rong Wen-Rui Xie | 2008 | World Journal of Gastroenterology2008，14，30： | 18 |
| 9 | Recurrent gain-of-function USP8 mutations in Cushing's disease显示文摘库欣的疾病,引起过量皮质醇生产的也已知的同样促肾上腺皮质的荷尔蒙(ACTH )-secreting 垂体腺瘤(舞步) ,占多达 85%corticotrophin 依赖的库欣的症候群盒子。然而,在这疾病的基因改变是不清楚的。这里,我们执行了从 12 秘密 ACTH 舞步导出并且匹配血样品的 DNA 定序的 whole-exome,它在候选人基因揭示了体的变化的三种类型, USP8 (编码 ubiquitin 特定的朊酶 8 ) ,专门在 exon 14 在 12 秘密 ACTH 舞步中的 8 个。我们进一步评估了体的 USP8 变化在另外由定序的 Sanger 的 258 舞步。进一步定序指向在 108 秘密 ACTH 舞步(62.04%) 中的 67 个识别了 USP8 变体的 17 种类型的一个总数。然而,任何一个都没在舞步的另外的类型这些变化被检测(n = 150 ) 。这些变化在 USP8 的 14-3-3 绑定主题以内聚集并且破坏在 USP8 和 14-3-3 蛋白质之间的相互作用,导致保护 EGFR 免受 lysosomal 降级的伤害的一个提高的能力。因此,有 EGFR 表示,提高的 EGFR 蛋白质丰富和 mRNA 表示的更高的发生 POMC 铺平的变异的 USP8 显示的舞步,它编码 ACTH 的先锋。有变异的 USP8 的舞步在尺寸是显著地更小的并且比野类型的舞步有更高的 ACTH 生产。在里面通过手术 resected 主要变异 USP8 的肿瘤房间, USP8 击倒或堵住的 EGFR 有效地稀释 ACTH 分泌物。一起拿,体的 gain-of-function USP8 变化是普通的并且在库欣的疾病贡献 ACTH 生产过剩。USP8 或 EGFR 的抑制为对待变异 USP8 的 corticotrophin 腺瘤是有希望的。我们的学习在库欣的疾病加亮潜在地功能的变异的基因并且提供卓见进这疾病的治疗学。 | Zeng-Yi Ma Zhi-Jian Song Jian-Hua Chen Yong-Fei Wang Shi-Qi Li Liang-Fu Zhou Ying Mao Yi-Ming Li Rong-Gui Hu Zhao-Yun Zhang Hong-Ying Ye Ming Shen Xue-Fei Shou Zhi-Qiang Li Hong Peng Qing-Zhong Wang Dai-Zhan Zhou Xiao-Lan Qin Jue Ji Jie Zheng Hong Chen Yin Wang Dao-Ying Geng Wei-Jun Tang Chao-Wei Fu Zhi-Feng Shi Yi-Chao Zhang Zhao Ye Wen-Qiang He Qi-Lin Zhang Qi-Sheng Tang Rong Xie Jia-Wei Shen Zu-Jia Wen Juan Zhou Tao Wang Shan Huang Hui-Jia Qiu Ni-Dan Qiao Yi Zhang Li Pan Wei-Min Bao Ying-Chao Liu Chuan-Xin Huang Yong-Yong Shi Yao Zhao | 2015 | Cell Research2015，25，3： | 16 |
| 10 | Prevalence, awareness, treatment, and control of hypertension in the non-dialysis chronic kidney disease patients显示文摘 | ZHENG Ying CAI Guang-yan CHEN Xiang-mei FU Ping CHEN Jiang-hua DING Xiao-qiang YU Xue-qing LIN Hong-li LIU Jian XIE Ru-juan WANG Li-ning NI Zhao-hui LIU Fu-you YIN Ai-ping XING Chang-ying WANG Li SHI Wei LIU Jian-she HE Ya-ni DING Guo-hua LI Wen-ge WU Guang-li MIAO Li-ning CHEN Nan SU Zhen MEI Chang-lin ZHAO Jiu-yang GU Yong BAI Yun-kai LUO Hui-min LIN Shan CHEN Meng-hua GONG Li YANG Yi-bin YANG Xiao-ping LI Ying WAN Jian-xin WANG Nian-song LI Hai-ying XI Chun-sheng HAO Li XU Yan FANG Jing-ai LIU Bi-cheng LI Rong-shan WANG Rong ZHANG Jing-hong WANG Jian-qin LOU Tan-qi SHAO Feng-min MEI Feng LIU Zhi-hong YUAN Wei-jie SUN Shi-ren ZHANG Ling ZHOU Chun-hua CHEN Qin-kai JIA Shun-lian GONG Zhi-feng GUAN Guang-ju XIA Tian ZHONG Liang-bao | 2013 | Chinese Medical Journal2013，，12： | 16 |
| 11 | Developmental Genetics Analysis for Plant Height in indica Hybrid Rice Across Environments显示文摘The developmental genetics of plant height was analyzed from two groups of three-line indica hybrid rice at two environmental conditions based on the NCII design,using the additive-dominant developmental genetics models and the statistic methods.The results showed that the rice genotypes and environmental conditions could both affect plant height,and the effects of environment on plant height decreased gradually with plant development.Additive and dominant effects both governed the performance of plant height at all developmental stages.However,the degrees of effect varied among the rice genotypes.Moreover,the interaction between environments and genotypes also affected plant height.The genetic effects differed at most developmental stages.Furthermore,the expression of additive effect was more active than that of dominant effect.Conditional interaction effects with environment also influenced plant height during genetic development,especially at the early stage.Mid-parent heterosis(HMP) increased gradually with the developmental stage of plant height,and maximized at the latest stage,whereas the heterosis over the better parent(HBP) showed small differences among the genotypes,and kept stable at the later stage,with positive numeric value.At most developmental stages,conditional HMP was positively significant,while conditional HBP was negatively significant.All above results suggest that HMP and HBP have some new expressions in all developmental periods and the levels and directions are quite different. | PENG Tao ZHONG Bing-qiang LING Ying-hua YANG Zheng-lin ZHA Ren-ming XIE Rong LIU Cheng-yuan HE Guang-hua | 2009 | Rice science2009，16，2： | 14 |
| 12 | IRF5 regulates lung macrophages M2 polarization during severe acute pancreatitis in vitro显示文摘AIM To investigate the role of interferon regulatory factor 5(IRF5) in reversing polarization of lung macrophages during severe acute pancreatitis(SAP) in vitro.METHODS A mouse SAP model was established by intraperitoneal(ip) injections of 20 μg/kg body weight caerulein. Pathological changes in the lung were observed by hematoxylin and eosin staining. Lung macrophages were isolated from bronchoalveolar lavage fluid. The quantity and purity of lung macrophages were detectedby fluorescence-activated cell sorting and evaluated by real-time polymerase chain reaction(RT-PCR). They were treated with IL-4/IRF5 specific siR NA(IRF5 siR NA) to reverse their polarization and were evaluated by detecting markers expression of M1/M2 using RTPCR.RESULTS SAP associated acute lung injury(ALI) was induced successfully by ip injections of caerulein, which was confirmed by histopathology. Lung macrophages expressed high levels of IRF5 as M1 phenotype during the early acute pancreatitis stages. Reduction of IRF5 expression by IRF5 siR NA reversed the action of macrophages from M1 to M2 phenotype in vitro. The expressions of M1 markers, including IRF5(S + IRF5 siR NA vs S + PBS, 0.013 ± 0.01 vs 0.054 ± 0.047, P < 0.01), TNF-α(S + IRF5 siR NA vs S + PBS, 0.0003 ± 0.0002 vs 0.019 ± 0.018, P < 0.001), iN OS(S + IRF5 siR NA vs S + PBS, 0.0003 ± 0.0002 vs 0.026 ± 0.018, P < 0.001) and IL-12(S + IRF5 si RNA vs S + PBS, 0.000005 ± 0.00004 vs 0.024 ± 0.016, P < 0.001), were decreased. In contrast, the expressions of M2 markers, including IL-10(S + IRF5 siR NA vs S + PBS, 0.060 ± 0.055 vs 0.0230 ± 0.018, P < 0.01) and Arg-1(S + IRF5 siR NA vs S + PBS, 0.910 ± 0.788 vs 0.0036 ± 0.0025, P < 0.001), were increased. IRF5 si RNA could reverse the lung macrophage polarization more effectively than IL-4.CONCLUSION Treatment with IRF5 siR NA can reverse the pancreatitisinduced activation of lung macrophages from M1 phenotype to M2 phenotype in SAP associated with ALI. | Kang Sun Song-Bing He Jian-Guo Qu Sheng-Chun Dang Ji-Xiang Chen Ai-Hua Gong Rong Xie Jian-Xin Zhang | 2016 | World Journal of Gastroenterology2016，22，42： | 13 |
| 13 | Equivalent thermal resistance minimization for a circular disc heat sink with reverting microchannels based on constructal theory and entransy theory显示文摘Thermal designs for microchannel heat sinks with laminar flow are conducted numerically by combining constructal theory and entransy theory. Three types of 3-D circular disc heat sink models, i.e. without collection microchannels, with center collection microchannels, and with edge collection microchannels, are established respectively. Compared with the entransy equivalent thermal resistances of circular disc heat sink without collection microchannels and circular disc heat sink with edge collection microchannels, that of circular disc heat sink with center collection microchannels is the minimum, so the overall heat transfer performance of circular disc heat sink with center collection microchannels has obvious advantages. Furthermore, the effects of microchannel branch number on maximum thermal resistance and entransy equivalent thermal resistance of circular disc heat sink with center collection microchannels are investigated under different mass flow rates and heat fluxes. With the mass flow rate increasing, both the maximum thermal resistances and the entransy equivalent thermal resistances of heat sinks with respective fixed microchannel branch number all gradually decrease. With the heat flux increasing, the maximum thermal resistances and the entransy equivalent thermal resistances of heat sinks with respective fixed microchannel branch number remain almost unchanged. With the same mass flow rate and heat flux, the larger the microchannel branch number, the smaller the maximum thermal resistance. While the optimal microchannel branch number corresponding to minimum entransy equivalent thermal resistance is 6. | WANG Liang XIE ZhiHui CHEN LinGen WANG Rong FENG HuiJun | 2021 | Science China(Technological Sciences)2021，64，1： | 11 |
| 14 | Clinical Characteristics of Children with Coronavirus Disease 2019 in Hubei, China显示文摘Since December 2019,COVID-19 has occurred unexpectedly and emerged as a health problem worldw ide.Despite the rapidly increasing number of cases in subsequent weeks,the clinical characteristics of pediatric cases are rarely described.A cross-sectional multicenter study was carried out in 10 hospitals across Hubei province.A total of 25 confirmed pediatric cases of COVID-19 were collected.The demographic data,epidemiological history,underlying discascs,clinical mani festations,laboratory and radiological data,treatments,and outcomes were analyzcd.Of 25 hospitalized patients with COVID-19,the boy to girl ratio was 1.27:1.The median age was 3 years.COVID-19 cases in children aged<3 years,3-6 years,and≥6-years patients were 10(40%),6(24%),and 9(36%),respectively.The most common symptoms at onset of ilness were fever(13[52%]),and dry cough(11[44%]).Chest CT images showed essential normal in 8 cases(33.3%),unilateral involvement of lungs in 5 cases(20.8%),and bilateral involvement in 11 cases(45.8%).Clinical diagnoses included upper respiratory tract infection(n=8),mild pneumonia(n=15),and critical cases(n=2).Two critical cases(8%)were given invasive mechanical ventilation,corticosteroids,and immunoglobulin.The symptoms in 24(96%)of 25 patients were alleviated and one patient had been discharged.It was concluded that children were susceptible to COVID-19 like adults,while the clinical presentations and outcomes were more favorable in children.However,children less than 3 years old accounted for majority cases and critical cases lied in this age group,which demanded extra attentions during home caring and hospitalization treatment. | Fang ZHENG Chun LIAO Qi-hong FAN Hong-bo CHEN Xue-gong ZHAO Zhong-guo XIE Xi-lin LI Chun-xi CHEN Xiao-xia LU Zhi-sheng LIU Wei LU Chun-bao CHEN Rong JIAO Ai-ming ZHANG Jin-tang WANG Xi-wei DING Yao-guang ZENG Li-ping CHENG Qing-feng HUANG Jiang WU Xi-chang LUO Zhu-jun WANG Yan-yan ZHONG Yan BAI Xiao-yan WU Run-ming JIN | 2020 | Current Medical Science2020，40，2： | 10 |
| 15 | Application of Two-Dimensional Electrophoresis in the Research of Retinal Proteins of Diabetic Rat显示文摘Diabetes mellitus (DM) is a chronic disease which is associated with numerous serious health complications such as diabetic retinopathy, and is the leading cause of new cases of blindness in adults at the age of 20-74 years old. The aim of the study was to establish and optimize a two-dimensional polyacrylamide gel electrophoresis (2-DE) technique for retina proteomics to improve the resolution and reproducibility, and to observe the proteomic changes of retinal tissues in diabetic and normal rats. Proteins were extracted from retinal tissues of normal and 8 weeks diabetic SD rats and used in two-dimensional electrophoresis. Various conditions of retina proteomic 2-DE were adjusted, optimized and protein spots of differential expression were obtained through analysis of 2-DE images with PDQuest software. By choosing appropriate sample amount, using pre-cast IPG dry strips (pH 5-8) and casting 12% equal gel, satisfactory 2-DE images of retina were obtained and a steady 2-DE technique was established. In this way, we found 36 spots in 2-DE gel of diabetic retinas that exhibited statistically significant variations, including up-regulation of 5 proteins in diabetic rat retinas, down-regulation of 23, and disappearance of 8, in comparison with normal tissues. The differences of protein expression were observed in retinas between diabetic and normal rats. Our established 2-DE technique of retina proteins could be effectively applied in proteomics of retina diseases. | Shangqing Liu Yanyan Zhang Xianyong Xie Weiming Hu Rong Cai Jian Kang Huijun Yang | 2007 | Cellular & Molecular Immunology2007，4，1： | 9 |
| 16 | Developmental Changes of the LPL mRNA Expression and Its Effect on IMF Content in Sheep Muscle显示文摘This study investigates the developmental changes of the lipoprotein lipase (LPL) mRNA level in sheep muscle and its effect on intramuscular fat (IMF) accumulation. Male Kazak and Xinjiang Merino sheep at 2-120 days old were selected. Six animals of each breed were slaughtered at 2,30,60,90,and 120 days (only the Xinjiang Merino sheep at 120-day old were available) to collect samples from longissimus dorsi muscle for the purpose of determining the IMF content and extracting total RNA that was used to investigate the developmental changes of the LPL mRNA expression by real-time PCR. The results showed that in male Kazak sheep,the IMF content increased with the progress of development and there were significant differences (P < 0.05) between the age groups. However,there was no difference (P > 0.05) between age groups in Xinjiang Merino sheep. Furthermore,the IMF content of the male Kazak sheep was significantly higher (P < 0.01) than that of the Xinjiang Merino sheep aged from 30 to 90 days. The highest LPL mRNA expression appeared at day 2 and it was significantly higher than that of all other age groups (P < 0.01),while animals at 60-day old had the lowest LPL mRNA expression in the male Kazak sheep. In Xinjiang Merino sheep,the highest one occurred at 30-day old (P < 0.01),followed by a continuous decrease to the lowest level at 90-day old,and then it started to increase slightly. At 2 to 60-day old,the LPL mRNA expression was negatively correlated to the IMF content (r = -0.625,P < 0.05) in male Kazak sheep,but no such relationship was detected in the male Xinjiang Merino sheep. | QIAO Yong HUANG Zhi-guo LI Qi-fa LIU Zhen-shan DAI Rong PAN Zeng-xiang XIE Zhuang LIU Hong-lin | 2008 | Agricultural Sciences in China2008，7，1： | 9 |
| 17 | Therapeutic Potential of Naja Naja Atra Venom in A Rat Model of Diabetic Nephropathy显示文摘Objective To study the protective effects of naja naja atra venom (NNAV) in a rat model of diabetic nephropathy (DN). Methods The rat diabetes model was induced by intraperitoneal injection of streptozotocin (STZ). Thirty-two model rats were randomly divided into one DN group (n=8) and three treatment groups (n=8 each) that received NNAV at doses of 30, 90, or 270 μg/(kg day) via oral gavage, another eight rats as normal controls. After 12 weeks, all rats were sacrificed and the changes in serum and urine biological index levels were determined by colorimetric assay. Microalbumin (mALB), N-acetyl-β- glucosaminidase (NAG) and cystatin C (CysC) concentrations were measured by ELISA. Renal tissues were sliced for pathological and immunohistochemical observations. Results Comparied with the DN group, serum glucose was decreased by 31.04%, total cholesterol 21.96%, triglyceride 23.78%, serum creatinine 19.83%, blood urea nitrogen 31.28%, urinary protein excretion 45.42%, mALB 10.42%, NAG 20.65%, CysC 19.57%, whereas albumin increased by 5.55%, high-density lipoprotein–cholesterol 59.09%, creatinine clearance 19.05% in the treatment group by NNAV administration at dose of 90 μg/(kg day). NNAV also reduced the levels of malondialdehyde in serum (22.56%) and kidney tissue (9.79%), and increased superoxide dismutase concentration in serum (15%) and decreased it in renal tissue (8.85%). In addition, under light microscopy kidney structure was improved and glomerular hypertrophy decreased by 8.29%. As shown by immunohistochemistry, NNAV inhibited transforming growth factor-β1 by 6.70% and nuclear actor-κB by 5.15%. Conclusion NNAV improves biological indexes in DN, and it may exert renoprotective effects in rats with STZ-induced diabetes. | DAI Gui Li HE Jing Kang XIE Yan HAN Rong QIN Zheng Hong ZHU Lu Jia | 2012 | Biomedical and Environmental Sciences2012，25，6： | 9 |
| 18 | Ubiquitylation of p62/sequestosome1 activates its autophagy receptor function and controls selective autophagy upon ubiquitin stress显示文摘在细胞的 ubiquitin (Ub ) 的改变动态平衡,作为 Ub 知道强调,展示并且影响处于多重条件的细胞的回答,然而,内在的机制不完全地被理解。这里,我们报导 autophagy 受体 p62/sequestosome-1 与结合酶, UBE2D2 和 UBE2D3 的 E2 Ub 交往。内长的 p62 在 Ub 动态平衡的 upregulation 期间经历 E2 依赖的 ubiquitylation,一个条件作为 Ub + 应力称为,那对由 bortezomib 的 Ub overexpression,热吃惊或延长 proteasomal 抑制内在,化学疗法的药。p62 的 Ubiquitylation 破坏 p62 的 UBA 领域的 dimerization,解放它的能力认出为选择 autophagy 的 polyubiquitylated 货物。我们进一步证明这机制可能为在 Ub + 压力条件之上的 autophagy 激活是批评的。机制的描述和在察觉到 Ub 应力并且控制选择 autophagy 的 p62 的规章的角色能帮助理解并且调制细胞的回答到许多内长、环境的挑战,潜在地对 autophagy 相关的病为治疗学的策略的发展打开一条新大街。 | Hong Peng Jiao Yang Guangyi Li Qing You Wen Han Tianrang Li Darning Gao Xiaoduo Xie Byung-Hoon Lee Juan Du Jian Hou Tao Zhang Hai Rao Ying Huang Qinrun Li Rong Zeng Lijian Hui Hongyan Wang Qin Xia Xuemin Zhang Yongning He Masaaki Komatsu Ivan Dikic Daniel Finley Ronggui Hu | 2017 | Cell Research2017，27，5： | 9 |
| 19 | Fourth C hinese National Consensus Report on the management of H elicobacter pylori infection显示文摘 | Wen Zhong Liu Yong Xie Hong Cheng Nong Hua Lu Fu Lian Hu Wan Dai Zhang Li Ya Zhou Ye Chen Zhi Rong Zeng Chong Wen Wang Shu Dong Xiao Guo Zong Pan Pin Jin Hu | 2013 | Journal of Digestive Diseases2013，，5： | 9 |
| 20 | Nasopharyngeal adenoid cystic carcinoma: magnetic resonance imaging features in ten cases显示文摘Nasopharyngeal adenoid cystic carcinoma (NACC) is a rare malignancy with high local invasiveness. To date, there is no consensus on the imaging characteristics of NACC. To address this, we retrospectively reviewed 10 cases of NACC and summarized the magnetic resonance imaging (MRI) features. MR images of 10 patients with histologically validated NACC were reviewed by two experienced radiologists. The location, shape, margin, signal intensity, lesion texture, contrast enhancement patterns, local invasion, and cervical lymphadenopathy of all tumors were evaluated. Clinical and pathologic records were also reviewed. No patients were positive for antibodies against Epstein-Barr virus (EBV). The imaging patterns of primary tumors were classified into two types as determined by location, shape, and margin. Of all patients, 7 had tumors with a type 1 imaging pattern and 3 had tumors with a type 2 imaging pattern. The 4 tubular NACCs were all homogeneous tumors, whereas 3 (60%) of 5 cribriform NACCs and the sole solid NACC were heterogeneous tumors with separations or central necrosis on MR images. Five patients had perineural infiltration and intracranial involvement, and only 2 had cervical lymphadenopathy. Based on these results, we conclude that NACC is a local, aggressive neoplasm that is often negative for EBV infection and associated with a low incidence of cervical lymphadenopathy. Furthermore, MRI features of NACC vary in locations and histological subtypes. | Xue-Wen Liu Chuan-Miao Xie Hui Li Rong Zhang Zhi-Jun Geng Yun-Xian Mo Jing Zhao Mu-Yan Cai Yan-Chun Lv Pei-Hong Wu | 2012 | Chinese Journal of Cancer2012，31，1： | 8 |
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