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| 1 | Tobacco RING E3 Ligase NtRFP1 Mediates Ubiquitination and Proteasomal Degradation of a Geminivirus-Encoded βC1显示文摘西红柿黄叶卷屈中国联系病毒的 betasatellite 编码的 C1 蛋白质作为一个致病力决定因素工作。更好理解 C1 在致病力工作的分子的基础,酵母一个烟草 cDNA 图书馆的二混血儿的屏幕作为诱饵用 C1 被执行。屏幕表明 C1 与烟草戒指手指蛋白质交往指明了 NtRFP1,它被双分子的荧光互补和 co-immunoprecipitation 试金进一步在 Nicotiana benthamiana 房间证实。NtRFP1 的表示被 C1 导致,并且在 vitro ubiquitination,试金证明 NtRFP1 是调停的功能的 E3 ubiquitin ligase C1 ubiquitination。另外, C1 被显示是在 vivo 的 ubiquitinated 并且由植物 26S proteasome 降级。在病毒的感染以后,植物 overexpressing NtRFP1 开发了稀释症状,而有 NtRFP1 的 silenced 表示的植物显示出严重症状。证据的另外的线证明 NtRFP1 通过由 26S proteasome 支持它的降级稀释导致 C1 的症状。一起拿,我们的结果建议烟草戒指 E3 ligase NtRFP1 由与 C1 交往调停稀释疾病症状它经由 ubiquitin/26S proteasome 系统的 ubiquitination 和降级。 | Qingtang Shen Tao Hu Min Bao Linge Cao Huawei Zhang Fengmin Song Qi Xie Xueping Zhou | 2016 | Molecular Plant2016，9，6： | 8 |
| 2 | Active dry yeast supplementation improves the growth performance,rumen fermentation, and immune response of weaned beef calves显示文摘The objective of this experiment was to investigate the potential benefits of active dry yeast(ADY)on the growth performance,rumen fermentation,nutrient digestibility,and serum parameters of weaned beef calves.Thirty Simmental crossbred male calves(body weight=86.47±4.41 kg and 70±4 d of age)were randomly divided into 2 groups:control(CON)(fed basal ration)and ADY(fed basal ration and 5 g/d ADY per calf).The dietary concentrate-to-roughage ratio was 35:65.All the calves were regularly provided rations 3 times a day at 07:00,13:00,and 19:00 and had free access to wate r.The experiment lasted for 60 d.The average daily gain of ADY group was higher(P=0.007)than that of the CON group,and the ratio of feed intake to average daily gain in the ADY group was reduced(P=0.022)as compared to the CON group.The concentration of ruminal ammonia-N was higher(P=0.023)in the CON group than that in the ADY group,but an opposite trend of microbial protein was found between the 2 groups.Also,the ruminal concentrations of propionate and butyrate were higher(P<0.05)in the ADY group than those in the CON group.Calves fed ADY exhibited higher(P<0.05)crude protein and neutral detergent fiber digestibility.Supplementation of ADY increased(P<0.05)the contents of glucose,glutathione peroxidase,superoxide dismutase,immunoglobulin A,immunoglobulin M,and interleukin 10 in the serum of calves,but an opposite trend was observed in malondialdehyde,interleukin 1 beta,and tumor necrosis factor alpha contents between the 2 groups.In conclusion,dietary supplementation with ADY could improve the growth performance,rumen fermentation,nutrient digestibility,antioxidant ability,and immune response of weaned beef calves. | Jian Ma Cheng Wang Zhisheng Wang Guang Cao Rui Hu Xueying Wang Huawei Zou Kun Kang Quanhui Peng Bai Xue Lizhi Wang Yueming Zhu Xiaopeng Zhu | 2021 | Animal Nutrition2021，，4： | 7 |
| 3 | Simultaneous gene editing of three homoeoalleles in self-incompatible allohexaploid grasses显示文摘Clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)system has been widely used for precise gene editing in plants.However,simultaneous gene editing of multiple homoeoalleles remains challenging,especially in self-incompatible polyploid plants.Here,we simultaneously introduced targeted mutations in all three homoeoalleles of two genes in the self-incompatible allohexaploid tall fescue,using both CRISPR/Cas9 and LbCas12a(LbCpf1)systems.Loss-of-function mutants of FaPDS exhibited albino leaves,while knockout of FaHSP17.9 resulted in impaired heat resistance in T0 generation of tall fescue.Moreover,these mutations were inheritable.Our findings demonstrate the feasibility of generating loss-of-function mutants in T0 generation polyploid perennial grasses using CRISPR/Cas systems. | Liang Zhang Tao Wang Guangyang Wang Aoyue Bi Misganaw Wassie Yan Xie Liwen Cao Huawei Xu Jinmin Fu Liang Chen Yang Zhao Tao Hu | 2021 | Journal of Integrative Plant Biology2021，63，8： | 3 |
| 4 | Robust Watermarking of Mobile Video Resistant against Barrel Distortion显示文摘In head mounted display(HMD),in order to cancel pincushion distortion,the images displayed on the mobile should be prewarped with barrel distortion.The copyright of the mobile video should be verified on both the original view and the pre-warped virtual view.A robust watermarking resistant against barrel distortion for HMDs is proposed in this paper.Watermark mask is embedded into image in consideration of imperceptibility and robustness of watermarking.In order to detect watermark from the pre-warped image with barrel distortion,an estimation method of the barrel distortion is proposed for HMDs.Then,the same warp is enforced on the embedded watermark mask with the estimated parameters of barrel distortion.The correlation between the warped watermark and the pre-warped image is computed to predicate the existence of watermark.As shown in experimental results,watermark of mobile video can be detected not only from the original views,but also from the pre-warped virtual view.It also shows that the proposed scheme is resistant against combined barrel distortion and common post-processing,such as JPEG compression. | Huawei Tian Yanhui Xiao Gang Cao Jianwei Ding Bo Ou | 2016 | China Communications2016，13，9： | 2 |
| 5 | The detection of micrometastases in the peripheral blood of patients with breast cancer for hSBEM mRNA and CD44V6 mRNA显示文摘Objective: Successful treatment of breast cancer greatly depends on the early detection of its metastasis, there- fore a sensitive and specific biomarker for detecting dissemination of the cancer cells will help to achieve this goal. This study was to evaluate the prognostic significance of human small breast epithelial mucin (hSBEM) and CD44V6 in breast cancer. Methods: The expressions of hSBEM mRNA and CD44V6 mRNA were detected with nested reverse transcription polymerase chain reaction (nested RT-PCR) in 67 samples of breast cancer and adjacent normal breast tissue, 16 samples of breast benign lesions tissue, and 67 specimens of peripheral blood from patients with breast cancer, 16 specimens of benign breast lesions, 20 specimens of healthy volunteers, and 25 (each five cases) other carcinomas tissue samples, including those of gastric carcinoma, colorectal carcinoma, esophageal carcinoma, lung carcinoma, and ovary carcinoma, were ana- lyzed for hSBEM mRNA expression by nested RT-PCR. Results: hSBEM mRNA expression was observed in 62/67 (92.54%) of breast cancer, 14/16 (87.50%) of breast benign lesions and 59/67 (88.05%) of normal breast tissue, with no significant differences between them (P > 0.05). None of the samples from other cancer tissues were positive. In peripheral blood the expression of hSBEM mRNA was detected in 34/67 (50.75%) from patients with breast cancer, with significant increasing (P < 0.05) in the cases of metastatic disease (stage IV) and those with lymph node metastasis compared with localized disease (stage I, II and III) and without lymph node metastasis, but its expression was not found in peripheral blood of patients with benign breast lesions or healthy volunteers. Although CD44V6 mRNA was significantly higher in breast cancer than in benign breast lesions tissue and normal breast tissue, its expression in peripheral blood show no significant difference (P > 0.05) in the patients with breast cancer (82.09%), benign breast lesion (75.00%), or healthy volunteers (70.00%). The expressions of hSBEM mRNA and CD44V6 mRNA had no correlation with the age of the patients, size of primary tumor, histological type and estrogen or progestin receptor status (P > 0.05). Conclusion: hSBEM mRNA, as assessed by nested RT-PCR, shows a mammary-specific and mammary-sensitive expression, and is a sensitive indicator of hematogeneous spread of breast cancer cell, while CD44V6 shows low sensitivity and specificity in detecting dissemination of breast cancer cell in peripheral blood. hSBEM mRNA is a promising molecular biomarker for detecting breast cancer micrometastases. | Jianlun Liu Huawei Yang Ji Cao Nanwu Yang | 2008 | The Chinese-German Journal of Clinical Oncology2008，7，1： | 2 |
| 6 | Attacking contrast enhancement forensics in digital images显示文摘Currently,plenty of digital image forensic techniques have been proposed and used as diagnostic tools.It is urgent and significant to assess the reliability of such techniques applied in practical scenarios.In this paper,we investigate the security of existing digital image contrast enhancement(CE)forensic algorithms.From the standpoint of attackers,we propose two types of attacks,CE trace hiding attack and CE trace forging attack,which could invalidate the forensic detector and fabricate two types of forensic errors,respectively.The CE trace hiding attack is implemented by integrating local random dithering into the design of pixel value mapping.The CE trace forging attack is proposed by modifying the gray level histogram of a target pixel region to counterfeit peak/gap artifacts.Such trace forging attack is typically applied to create sophisticated composite images which could deceive the prior CE-based composition detectors.Extensive experimental results demonstrate the efficacy of our proposed CE anti-forensic schemes. | CAO Gang ZHAO Yao NI RongRong TIAN HuaWei YU LiFang | 2014 | Science China(Information Sciences)2014，57，5： | 1 |
| 7 | Influence of a TiAlN Coating on the Mechanical Properties of a Heat Resistant Steel at Room Temperature and 650℃显示文摘A TiAlN coating was deposited on a heat resistant steel X12CrMoWVNbN10-1-1 by vacuum arc ion plating.The tensile and fatigue properties of the coated steel were investigated at room temperature(RT) and 650 ℃.The results reveal that the TiAlN coating is compact,on which a small number of large particle and pits are present.The Ti/Al atomic ratio in the coating is about 0.94.The average hardness of the coating is 1 868 HV0.1 and the interface bonding force between TiAlN coating and the substrate is about 31 N.The elastic modulus and the strength of the steel are improved by the deposition of TiAlN coating.The influence of the TiAlN coating on the tensile properties of the steel can be ignored at both RT and 650 ℃.Moreover,there is no obvious decrease of the fatigue limit of substrate when the steel is coated by the coating at the investigated temperature. | 张程煜 CAO Huawei HAN Dong QIAO Shengru 郭永 | 2013 | Journal of Wuhan University of Technology(Materials Science)2013，28，5： | 1 |
| 8 | Reconfigurable,graphene-coated,chalcogenide nanowires with a sub-10-nm enantioselective sorting capability显示文摘Chiral surface plasmon polaritons(SPPs)produced by plasmonic nanowires can be used to enhance molecular spectroscopy for biosensing applications.Nevertheless,the switchable stereoselectivity and detection of various analytes are limited by a lack of switchable,chiral SPPs.Using both finite-element method simulations and analytic calculations,we present a graphene-coated chalcogenide(GCC)nanowire that produces mid-infrared,chiral SPPs.The chiral SPPs can be reversibly switched between“on”(transparent)and“off”(opaque)by non-volatile structural state transitions in the dielectric constants of the chalcogenide glass Ge2Sb2Te5.Furthermore,by controlling the Fermi energy of the graphene-coating layer,the nanowire can output either non-chiral or chiral SPPs.A thermal-electric model was built to illustrate the possibility of ultrafast on/off switching of the SPPs at the terminus of the nanowire.Finally,we show that a selective,lateral sorting of sub-10-nm enantiomers can be achieved via the GCC nanowire.Chiral nanoparticles with opposite handedness experience transverse forces that differ in both their sign and magnitude.Our design may pave the way for plasmonic nanowire networks and tunable nanophotonic devices,which require the ultrafast switching of SPPs,and provide a possible approach for a compact,enantiopure synthesis. | Tun Cao Long Tian Huawei Liang Kai-Rong Qin | 2018 | Microsystems & Nanoengineering2018，4，1： | 1 |
| 9 | Effects of Supplemental Carbohydrate Sources on Lactation Performance, Nitrogen Balance,and Ruminal Fermentation in Dairy Cows Fed Energy-Deficient Total Mixed Rations显示文摘The effects of supplemental carbohydrate (CHO) sources on milk yield and composition, nitrogen (N) balance,and ruminal fermentation were evaluated in lactating dairy cows fed energy-deficient total mixed rations (TMR) . Fifty-six lactating Holstein cows (36. 8 ± 3. 4 kg / d of milk yield; 152 ± 26 days in milk [DIM]) were randomly assigned to one of four diets: (1) basal TMR (control) ; (2) basal TMR + 1. 25 kg / d sucrose (SUC) ; (3) basal TMR + 2. 12 kg /d steam-flaked corn (SFC) ; (4) basal TMR + 2. 23 kg / d wheat (WHE) . The trial lasted 70 days,including the first 10 days of adaptation. Simultaneously, a 4 × 4 Latin square trial was conducted with four ruminally cannulated cows of similar DMI (Dry Matter Intake) fed on the above four diets. Each testing period lasted 18 days: 15 days for adaptation and 3 days for sampling. Cows fed SUC-,SFC-,and WHE-supplemented diets produced 1. 6,2. 9,and 0. 8 kg / d,respectively,more milk than those on the basal TMR control diet,but the difference was not significant (P = 0. 160) . However,the production of energy-corrected milk of cows fed CHO-supplemented diets improved significantly (P = 0. 020) ,and the yield of 4% fatcorrected milk tended to be higher (P = 0. 063) than in control animals. Percentages of milk protein,lactoseand solids-not-fat (SNF) increased significantly in cows fed CHO-supplemented diets (P < 0. 05) ,and yields of milk protein and SNF were significantly higher (P <0. 05) ; yields of milk fat and lactose were somewhat higher (P < 0. 10) . Cows receiving supplementary CHO diets consumed more DM (Dry Matter) (P = 0. 023) ; however,there was no significant difference in N retention,digestibility,or utilization efficiency among the treatments (P > 0. 10) . The average ruminal pH was lowest in cows supplemented with SUC,while that in cows supplemented with SFC and WHE was only slightly reduced (P = 0. 025) . Ruminal NH 3 -N concentration was highest with no supplementary CHO (P = 0. 017) ,and changes in postfeeding time were similar across the diets. Total volatile fatty acid concentration was significantly lower in control animals than in those on SUCand WHE-supplemented diets (P = 0. 001) ; significant dietary responses were observed in the concentrations of acetate,butyrate,and branchedchain fatty acids and in the acetate-to-propionate ratio (P <0. 001) . These results indicate that supplementation with different CHO sources in energy-deficient diets substantially improved the balance of energy and N in the rumen,altered ruminal fermentation,and improved lactation performance in dairy cows. | Huawei Su Zaijun Yang Shengli Li Zhijun Cao | 2011 | Journal of Animal Science and Biotechnology2011，2，3： | 0 |
| 10 | A high-performance multi-wavelength optical switch based on multiple Fano resonances in an all-dielectric metastructure显示文摘The multi-wavelength optical switch based on an all-dielectric metastructure consisting of four asymmetric semi-circular rings was designed and analyzed in this paper.Four Fano resonance modes,which can be explained by bound states in the continuum(BIC)theory,are excited in our structure with a maximum Q-factor of about 2450 and a modulation depth close to 100%.By changing the polarization direction of the incident light,the transmission amplitude of Fano resonances can get effectively modulated.Based on this tuning property,the metastructure can achieve a multi-wavelength optical switch in the near-infrared region(900—980 nm)and the maximum extinction ratio can reach 38.3 d B.In addition,the results indicate that the Fano resonances are sensitive to the changes in the refractive index.The sensitivity(S)and the figure of merit(FOM)are 197 nm/RIU and 492 RIU-1.The proposed metastructure has promising potential in applications such as optical switches,sensors,modulators and lasers. | CAO Shuangshuang FAN Xinye FANG Wenjing CHEN Huawei BAI Chenglin TONG Cunzhu | 2024 | Optoelectronics Letters2024，20，4： | 0 |
| 11 | User Profiling for CSDN:Keyphrase Extraction,User Tagging and User Growth Value Prediction显示文摘The Chinese Software Developer Network(CSDN)is one of the largest information technology communities and service platforms in China.This paper describes the user profiling for CSDN,an evaluation track of SMP Cup 2017.It contains three tasks:(1)user document keyphrase extraction,(2)user tagging and(3)user growth value prediction.In the first task,we treat keyphrase extraction as a classification problem and train a Gradient-Boosting-Decision-Tree model with comprehensive features.In the second task,to deal with class imbalance and capture the interdependency between classes,we propose a two-stage framework:(1)for each class,we train a binary classifier to model each class against all of the other classes independently;(2)we feed the output of the trained classifiers into a softmax classifier,tagging each user with multiple labels.In the third task,we propose a comprehensive architecture to predict user growth value.Our contributions in this paper are summarized as follows:(1)we extract various types of features to identify the key factors in user value growth;(2)we use the semi-supervised method and the stacking technique to extend labeled data sets and increase the generality of the trained model,resulting in an impressive performance in our experiments.In the competition,we achieved the first place out of 329 teams. | Guoliang Xing Hao Gao Qi Cao Xinyu Yue Bingbing Xu Keting Cen Huawei Shen | 2019 | Data Intelligence2019，1，2： | 0 |
| 12 | Integration of chemical and biological methods:A case study of polycyclic aromatic hydrocarbons pollution monitoring in Shandong Peninsula,China显示文摘Polycyclic aromatic hydrocarbons(PAHs),as persistent toxic substances(PTS),have been widely monitored in coastal environment,including seawater and sediment.However,scientific monitoring methods,like ecological risk assessment and integrated biomarker response,still need massive researches to verify their availabilities.This study was performed in March,May,August and October of 2018 at eight sites,Yellow River estuary(S1),Guangli Port(S2),Xiaying(S3),Laizhou(S4),Inner Bay(S5),Outer Bay(S6),Hongdao(S7)and Hongshiya(S8)of Shandong Peninsula,China.The contents of 16 priority PAHs in local seawater and sediment were determined,by which ecological risk assessment risk quotient(RQ)for seawater and sediment quality guidelines(SQGs)were calculated to characterize the PAHs pollution.Meanwhile,multiple biomarkers in the digestive gland of clam Ruditapes philippinarum were measured to represent different biological endpoints,including ethoxyresorufin-O-deethylase(EROD),glutathione S-transferase(GST),sulfotransferase(SULT),superoxide dismutase(SOD)and lipid peroxidation(LPO),by which integrated biomarker response(IBR)was calculated to provide a comprehensive assessment of environmental quality.Taken together,these results revealed the heaviest pollution at S2 as both PAHs concentrations and biomarkers responses reflected,and supported the integrated biomarker response as a useful tool for marine environmental monitoring,through its integration with SQGs. | Zeyuan Li Yunhao Cao Huawei Qin Yuanqing Ma Luqing Pan Jiawei Sun | 2022 | Journal of Environmental Sciences2022，34，1： | 0 |
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