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| 1 | Modified hyaluronic acid hydrogels with chemical groups that facilitate adhesion to host tissues enhance cartilage regeneration显示文摘Stable integration of hydrogel implants with host tissues is of critical importance to cartilage tissue engineering.Designing and fabricating hydrogels with high adhesive strength,stability and regeneration potential are major challenges to be overcome.This study fabricated injectable adhesive hyaluronic acid(HA)hydrogel modified by aldehyde groups and methacrylate(AHAMA)on the polysaccharide backbone with multiple anchoring mechanisms(amide bond through the dynamic Schiff base reaction,hydrogen bond and physical interpenetration).AHAMA hydrogel exhibited significantly improved durability and stability within a humid environment(at least 7 days),together with higher adhesive strength(43 KPa to skin and 52 KPa to glass),as compared to commercial fibrin glue(nearly 10 KPa)and HAMA hydrogel(nearly 20 KPa).The results showed that AHAMA hydrogel was biocompatible and could be easily and rapidly prepared in situ.In vitro cell culture experiments showed that AHAMA hydrogel could enhance proliferation(1.2-folds after 3 days)and migration(1.5-folds after 12 h)of bone marrow stem cells(BMSCs),as compared to cells cultured in a culture dish.Furthermore,in a rat osteochondral defect model,implanted AHAMA hydrogel significantly promoted integration between neo-cartilage and host tissues,and significantly improved cartilage regeneration(modified O’Driscoll histological scores of 16.0±4.1 and 18.3±4.6 after 4 and 12-weeks of post-implantation in AHAMA groups respectively,12.0±2.7 and 12.2±2.8 respectively in HAMA groups,9.8±2.4 and 11.5±2.1 respectively in untreated groups).Hence,AHAMA hydrogel is a promising adhesive biomaterial for clinical cartilage regeneration and other biomedical applications. | Jiaqing Chen Jiabei Yang Li Wang Xuewei Zhang Boon Chin Heng Dong-An Wang Zigang Ge | 2021 | Bioactive Materials2021，6，6： | 6 |
| 2 | Biological effect and molecular mechanism study of biomaterials based on proteomic research显示文摘Along with the role transformation of biomaterials from bioinert substitute to regenerative inducer, the biological effect and mechanism of material-organism interaction become more important. Since most of animal tests and cellular experiments stay on the phenomenon description instead of mechanism interpretation, the development of proteomics technologies provides a golden opportunity to uncover the molecular interaction mechanism between biomaterial-organism on whole scale. This review summarizes current application of proteomics in biological effect and mechanism study of biomaterials, and discusses the development and challenges for future studies. | Zhen Zhen Yufeng Zheng Zigang Ge Chen Lai Tingfei Xi | 2017 | Journal of Materials Science & Technology2017，33，7： | 1 |
| 3 | Architecture design of GPS software receiver and implementation of its acquisition algorithm with fine frequency estimation 显示文摘 | Zhu Xuefen Chen Xiyuan Li Zigang | 2008 | Journal of Southeast University2008，24，1： | 1 |
| 4 | EBV encoded miR-BHRF1-1 potentiates viral lytic replication by downregulating host p53 in nasopharyngeal carcinoma显示文摘 | Zijian Li Xue Chen Lili Li Sufang Liu Lifang Yang Xiaoqian Ma Min Tang Ann M. Bode Zigang Dong Lunquan Sun Ya Cao | 2011 | International Journal of Biochemistry and Cell Biology2011，，2： | 1 |
| 5 | Novel dual inhibitor for targeting PIM1 and FGFR1 kinases inhibits colorectal cancer growth in vitro and patient-derived xenografts in vivo显示文摘Colorectal cancer(CRC) is the second most common cause of cancer-related death in the world. The pro-viral integration site for Moloney murine leukemia virus 1(PIM1) is a proto-oncogene and belongs to the serine/threonine kinase family, which are involved in cell proliferation, migration,and apoptosis. Fibroblast growth factor receptor 1(FGFR1) is a tyrosine kinase that has been implicated in cell proliferation, differentiation and migration. Small molecule HCI-48 is a derivative of chalcone, a class of compounds known to possess anti-tumor, anti-inflammatory and antibacterial effects. However,the underlying mechanism of chalcones against colorectal cancer remains unclear. This study reports that HCI-48 mainly targets PIM1 and FGFR1 kinases, thereby eliciting antitumor effects on colorectal cancer growth in vitro and in vivo. HCI-48 inhibited the activity of both PIM1 and FGFR1 kinases in an ATPdependent manner, as revealed by computational docking models. Cell-based assays showed that HCI-48inhibited cell proliferation in CRC cells(HCT-15, DLD1, HCT-116 and SW620), and induced cell cycle arrest in the G2/M phase through modulation of cyclin A2. HCI-48 also induced cellular apoptosis, as evidenced by an increase in the expression of apoptosis biomarkers such as cleaved PARP, cleaved caspase 3 and cleaved caspase 7. Moreover, HCI-48 attenuated the activation of downstream components of the PIM1 and FGFR1 signaling pathways. Using patient-derived xenograft(PDX) murine tumor models,we found that treatment with HCI-48 diminished the PDX tumor growth of implanted CRC tissue expressing high protein levels of PIM1 and FGFR1. This study suggests that the inhibitory effect of HCI-48 on colorectal tumor growth is mainly mediated through the dual-targeting of PIM1 and FGFR1kinases. This work provides a theoretical basis for the future application of HCI-48 in the treatment of clinical CRC. | Fanxiang Yin Ran Zhao Dhilli Rao Gorja Xiaorong Fu Ning Lu Hai Huang Beibei Xu Hanyong Chen Jung-Hyun Shim Kangdong Liu Zhi Li Kyle Vaughn Laster Zigang Dong Mee-Hyun Lee | 2022 | Acta Pharmaceutica Sinica B2022，12，11： | 1 |
| 6 | Will the future of shale reservoirs lie in CO2 geological sequestration?显示文摘CO2 geological sequestration in a depleted shale gas reservoir is a promising method to address the global energy crisis as well as to reduce greenhouse gas emissions. Though improvements have been achieved by many researchers, the carbon sequestration and enhanced gas recovery(CS-EGR) in shale formations is still in a preliminary stage. The current research status of CO2 sequestration in shale gas reservoirs with potential EGR is systematically and critically addressed in the paper. In addition, some original findings are also presented in this paper. This paper will shed light on the technology development that addresses the dual problem of energy crisis and environmental degradation. | ZHAN Jie CHEN ZhangXin ZHANG Ying ZHENG ZiGang DENG Qi | 2020 | Science China(Technological Sciences)2020，63，7： | 1 |
| 7 | Reconstruction of intersecting curved solids from 2D orthographic views 显示文摘 | Fu Zigang Zou Beiji Chen Yiming | 2010 | Computer-Aided Design2010，42，9： | 1 |
| 8 | Three-component coupling of aldehyde, alkyne, and amine catalyzed by silver in ionic liquid显示文摘 | Zigang Li Chunmei Wei Liang Chen Rajender S. Varma Chao-Jun Li | 2004 | Tetrahedron Letters2004，，11： | 1 |
| 9 | Antioxidant properties of aspirin: Characterization of the ability of aspirin to inhibit silica-induced lipid peroxidation, DNA damage, NF-κB activation, and TNF-α production显示文摘 | Xianglin Shi Min Ding Zigang Dong Fei Chen Jiangping Ye Suwei Wang Stephen S. Leonard Vince Castronova Val Vallyathan | 1999 | Molecular and Cellular Biochemistry (-)1999，，1： | 1 |
| 10 | Hexafluoropropylene oxide trimer acid,a perfluorooctanoic acid alternative,induces cardiovascular toxicity in zebrafish embryos显示文摘As an increasingly used alternative to perfluorooctanoic acid(PFOA),hexafluoropropylene oxide trimer acid(HFPO-TA)has been widely detected in global water environments.However,little is known regarding its toxic effects on cardiovascular development.Here,zebrafish embryos were treated with egg water containing 0,60,120,or 240 mg/L HFPO-TA.Results showed that HFPO-TA treatment led to a significant reduction in both larval survival percentage and heart rate.Furthermore,HFPO-TA exposure caused severe pericardial edema and elongation of the sinus venous to bulbus arteriosus distance(SV-BA)in Tg(myl7:GFP)transgenic larvae,disrupting the expression of genes involved in heart development and thus causing abnormal heart looping.Obvious sprouting angiogenesis was observed in the 120 and 240 mg/L exposed Tg(fli:GFP)transgenic larvae.HFPO-TA treatment also impacted the mRNA levels of genes involved in the vascular endothelial growth factor(VEGF)pathway and embryonic vascular development.HFPO-TA exposure significantly decreased erythrocyte number in Tg(gata1:DsRed)transgenic embryos and influenced gene expression associated with the heme metabolism pathway.HFPO-TA also induced oxidative stress and altered the transcriptional levels of genes related to cell cycle and apoptosis,inhibiting cell proliferation while promoting apoptosis.Therefore,HFPO-TA exposure may induce abnormal development of the cardiovascular and hematopoietic systems in zebrafish embryos,suggesting it may not be a suitable or safe alternative for PFOA. | Sujie Sun Li Zhang Xue Li Lu Zang Ling Huang Junquan Zeng Zigang Cao Xinjun Liao Zilin Zhong Huiqiang Lu Jianjun Chen | 2024 | Journal of Environmental Sciences2024，，5： | 0 |
| 11 | Key considerations on the development of biodegradable biomaterials for clinical translation of medical devices:With cartilage repair products as an example显示文摘With the interdisciplinary convergence of biology,medicine and materials science,both research and clinical translation of biomaterials are progressing at a rapid pace.However,there is still a huge gap between applied basic research on biomaterials and their translational products-medical devices,where two significantly different perspectives and mindsets often work independently and non-synergistically,which in turn significantly increases financial costs and research effort.Although this gap is well-known and often criticized in the biopharmaceutical industry,it is gradually widening.In this article,we critically examine the developmental pipeline of biodegradable biomaterials and biomaterial-based medical device products.Then based on clinical needs,market analysis,and relevant regulations,some ideas are proposed to integrate the two different mindsets to guide applied basic research and translation of biomaterial-based products,from the material and technical perspectives.Cartilage repair substitutes are discussed here as an example.Hopefully,this will lay a strong foundation for biomaterial research and clinical translation,while reducing the amount of extra research effort and funding required due to the dissonance between innovative basic research and commercialization pipeline. | Li Wang Xiaolei Guo Jiaqing Chen Zhen Zhen Bin Cao Wenqian Wan Yuandong Dou Haobo Pan Feng Xu Zepu Zhang Jianmei Wang Daisong Li Quanyi Guo Qing Jiang Yanan Du Jiakuo Yu Boon Chin Heng Qianqian Han Zigang Ge | 2022 | Bioactive Materials2022，7，3： | 0 |
| 12 | A novel selective ERK1/2 inhibitor,Laxiflorin B,targets EGFR mutation subtypes in non-small-cell lung cancer显示文摘Extracellular regulated protein kinases 1/2(ERK1/2)are key members of multiple signaling pathways,including the ErbB axis.Ectopic ERK1/2 activation contributes to various types of cancer,especially drug resistance to inhibitors of RTK,RAF and MEK,and specific ERK1/2 inhibitors are scarce.In this study,we identified a potential novel covalent ERK inhibitor,Laxiflorin B,which is a herbal compound with anticancer activity.However,Laxiflorin B is present at low levels in herbs;therefore,we adopted a semi-synthetic process for the efficient production of Laxiflorin B to improve the yield.Laxiflorin B induced mitochondria-mediated apoptosis via BAD activation in non-small-cell lung cancer(NSCLC)cells,especially in EGFR mutant subtypes.Transcriptomic analysis suggested that Laxiflorin B inhibits amphiregulin(AREG)and epiregulin(EREG)expression through ERK inhibition,and suppressed the activation of their receptors,ErbBs,via a positive feedback loop.Moreover,mass spectrometry analysis combined with computer simulation revealed that Laxiflorin B binds covalently to Cys-183 in the ATP-binding pocket of ERK1 via the D-ring,and Cys-178 of ERK1 through non-inhibitory binding of the A-ring.In a NSCLC tumor xenograft model in nude mice,Laxiflorin B also exhibited strong tumor suppressive effects with low toxicity and AREG and EREG were identified as biomarkers of Laxiflorin B efficacy.Finally,Laxiflorin B-4,a C-6 analog of Laxiflorin B,exhibited higher binding affinity for ERK1/2 and stronger tumor suppression.These findings provide a new approach to tumor inhibition using natural anticancer compounds. | Cheng-Yao Chiang Min Zhang Junrong Huang Juan Zeng Chunlan Chen Dongmei Pan Heng Yang Tiantian Zhang Min Yang Qiangqiang Han Zou Wang Tian Xiao Yangchao Chen Yongdong Zou Feng Yin Zigang Li Lizhi Zhu Duo Zheng | 2024 | Acta Pharmacologica Sinica2024，45，2： | 0 |
| 13 | N6-methyladenosine facilitates mitochondrial fusion of colorectal cancer cells via induction of GSH synthesis and stabilization of OPA1 mRNA显示文摘Mitochondria undergo fission and fusion that are critical for cell survival and cancer development,while the regulatory factors for mitochondrial dynamics remain elusive.Herein we found that RNA m^(6)A accelerated mitochondria fusion of colorectal cancer(CRC)cells.Metabolomics analysis and function studies indicated that m^(6)A triggered the generation of glutathione(GSH)via the upregulation of RRM2B-a p53-inducible ribonucleotide reductase subunit with anti-reactive oxygen species potential.This in turn resulted in the mitochondria fusion of CRC cells.Mechanistically,m^(6)A methylation of A1240 at 3'UTR of RRM2B increased its mRNA stability via binding with IGF2BP2.Similarly,m^(6)A methylation of A2212 at the coding sequence(CDS)of OPA1-an essential GTPase protein for mitochondrial inner membrane fusion-also increased mRNA stability and triggered mitochondria fusion.Targeting m^(6)A through the methyltransferase inhibitor STM2457 or the dm^(6)ACRISPR system significantly suppressed mitochondria fusion.In vivo and clinical data confirmed the positive roles of the m^(6)A/mitochondrial dynamics in tumor growth and CRC progression.Collectively,m^(6)A promoted mitochondria fusion via induction of GSH synthesis and OPA1 expression,which facilitated cancer cell growth and CRC development. | Jiawang Zhou Haisheng Zhang Ke Zhong Lijun Tao Yu Lin Guoyou Xie Yonghuang Tan You Wu Yunqing Lu Zhuojia Chen Jiexin Li Xin Deng Qin Peng Zigang Li Hongsheng Wang | 2024 | National Science Review2024，11，3： | 0 |
| 14 | Repurposed benzydamine targeting CDK2 suppresses the growth of esophageal squamous cell carcinoma显示文摘Esophageal squamous cell carcinoma (ESCC) is one of the leading causes of cancer death worldwide. It is urgent to develop new drugs to improve the prognosis of ESCC patients. Here, we found benzydamine, a locally acting non-steroidal anti-inflammatory drug, had potent cytotoxic effect on ESCC cells. Benzydamine could suppress ESCC proliferation in vivo and in vitro. In terms of mechanism, CDK2 was identified as a target of benzydamine by molecular docking, pull-down assay and in vitro kinase assay. Specifically, benzydamine inhibited the growth of ESCC cells by inhibiting CDK2 activity and affecting downstream phosphorylation of MCM2, c-Myc and Rb, resulting in cell cycle arrest. Our study illustrates that benzydamine inhibits the growth of ESCC cells by downregulating the CDK2 pathway. | Yubing Zhou Xinyu He Yanan Jiang Zitong Wang Yin Yu Wenjie Wu Chenyang Zhang Jincheng Li Yaping Guo Xinhuan Chen Zhicai Liu Jimin Zhao Kangdong Liu Zigang Dong | 2023 | Frontiers of Medicine2023，17，2： | 0 |
| 15 | Caudal dorsal artery generates hematopoietic stem and progenitor cells via the endothelial-to-hematopoietic transition in zebrafish显示文摘Zebrafish hematopoietic stem and progenitor cells(HSPCs) originate from the hemogenic endothelium of the ventral wall of the dorsal aorta(DA) through the endothelial-to-hematopoietic transition(EHT) from approximately 30 to 60 hours post fertilization(hpf). However, whether other artery sites can generate HSPCs de novo remains unclear. In this study, using live imaging and lineage tracing, we found that the caudal dorsal artery(CDA) in the caudal hematopoietic tissue directly gave rise to HSPCs through EHT.This process initiated from approximately 60 hpf and terminated at approximately 156 hpf. Compared with that in the DA, fewer EHT events were observed in the CDA. The EHT events in the DA and CDA were similarly regulated by Runx1 but differentially influenced by blood flow(i.e., the EHT frequency in CDA was affected to a lesser extent when circulation was compromised in the tnnt2a^(-/-)mutant). Therefore,the whole artery, including both DA and CDA, was endowed with the ability to produce HSPCs during a much longer time period. Coincidently, the lineage tracing results indicated that adult hematopoietic cells originated from the embryonic endothelium, and those produced later preferentially colonized the adult thymus. Collectively, our study revealed that the CDA serves as an additional source of hematopoiesis, and it shows similar but not identical properties with the DA. | YANDong Zhan Youkui Huang Jingying Chen Zigang Cao Jianbo He Jingjing Zhang Honghui Huang Hua Ruan Lingfei Luo Li Li | 2018 | Journal of Genetics and Genomics2018，45，6： | 0 |
| 16 | A bifunctional vinyl-sulfonium tethered peptide induced by thio-Michael-type addition reaction显示文摘The modification and functionalization of peptides is of great significance in modern biotechnology and drug development. Here we report a highly reactive Michael-type warhead for the covalently modification of cysteine on peptide and protein. By installing a vinyl group onto a methionine residue of peptide,the produced vinyl sulfonium can be efficiently nucleophilic added by appropriate cysteine residue of this peptide, and thus yield a cyclized peptide. This peptide cyclization strategy was proven to exhibit improved cell penetration and good stability. Moreover, a peptide ligand bearing vinyl sulfonium could covalently bind to the cysteine in the target protein, indicating the potential of vinyl sulfonium as a novel warhead for developing covalent peptide inhibitor. | Hongkun Xu Xuan Qin Yaping Zhang Chuan Wan Rui Wang Zhanfeng Hou Xiaofeng Ding Hailing Chen Ziyuan Zhou Yang Li Chenshan Lian Feng Yin Zigang Li | 2022 | Chinese Chemical Letters2022，33，4： | 0 |
| 17 | Prediction of Potential Distribution of the Genus Cricotopus(Diptera:Chironomidae)Based on MaxEnt Model显示文摘The prediction of suitable area is a method for predicting the potential distribution by using the maximum entropy model.This study predicted the potential suitable habitats for the genus Cricotopus of Chironomidae in China.The latitude and longitude information of 98 distribution sites of Cricotopus in China and the biological environmental factors and altitude distribution in China were collected,and suitable habitats for Cricotopus were predicted,obtaining the suitable ranges and areas of Cricotopus in China,which is consistent with the known living conditions of Cricotopus.The study on the diversity of Cricotopus and the prediction of its suitable habitats provide a theoretical basis for Cricotopus in water monitoring and paddy fields,as well as basic data for the study on the genus Cricotopus. | Zigang XU Yuanyuan YAO Qing CHEN Panpan XIANG Jingru SHANGGUAN Shiwang LIU Yue FU | 2023 | Agricultural Biotechnology2023，12，6： | 0 |
| 18 | Dynamic multi-keyword fuzzy ranked search with leakage resilience over encrypted cloud data显示文摘To achieve the confidentiality and retrievability of outsourced data simultaneously,a dynamic multi-keyword fuzzy ranked search scheme(DMFRS)with leakage resilience over encrypted cloud data based on two-level index structure was proposed.The first level index adopts inverted index and orthogonal list,combined with 2-gram and location-sensitive Hashing(LSH)to realize a fuzzy match.The second level index achieves user search permission decision and search result ranking by combining coordinate matching with term frequency-inverse document frequency(TF-IDF).A verification token is generated within the results to verify the search results,which prevents the potential malicious tampering by cloud service providers(CSP).The semantic security of DMFRS is proved by the defined leakage function,and the performance is evaluated based on simulation experiments.The analysis results demonstrate that DMFRS gains certain advantages in security and performance against similar schemes,and it meets the needs of storage and privacy-preserving for outsourcing sensitive data. | Zhou Yousheng Huang Miao Liu Yuanni Chen Zigang | 2023 | The Journal of China Universities of Posts and Telecommunications2023，30，2： | 0 |
| 19 | Recent advances in chemical protein synthesis:method developments and biological applications显示文摘The central dogma of modern biology underscores the pivotal roles proteins play in diverse biological processes,the study of which necessitates advanced methods to produce proteins with precision and versatility.Chemical protein synthesis,a powerful approach utilizing chemical reactions for the de novo construction of structurally accurate proteins,has emerged as a transformative tool for studying proteins and generating protein derivatives/mimics inaccessible by natural biological machinery,including post-translationally modified proteins,proteins comprised of unnatural amino acids,as well as mirror-image proteins.This review summarizes recent strides in synthetic method developments for chemical protein synthesis,including innovative techniques in solid-phase peptide synthesis,the challenges presented by difficult sequences in either synthesis or folding and the exploration of novel ligation reactions using both chemical and enzymatic methods.Furthermore,the review also delves into newly developed protocols for site-selective protein modifications and the generation of stapled or macrocyclized peptides/miniproteins,highlighting the power of chemical methods to make structurally diverse proteins.Recent applications of synthetic proteins in investigating post-translational modifications(phosphorylation,lipidation,glycosylation,ubiquitination,etc.),mirror-image biological processes and drug development are further discussed.Together,these topics provide a comprehensive overview of the current landscape of chemical protein synthesis. | Suwei Dong Ji-Shen Zheng Yiming Li Huan Wang Gong Chen Yongxiang Chen Gemin Fang Jun Guo Chunmao He Honggang Hu Xuechen Li Yanmei Li Zigang Li Man Pan Shan Tang Changlin Tian Ping Wang Bian Wu Chuanliu Wu Junfeng Zhao Lei Liu | 2024 | Science China Chemistry2024，67，4： | 0 |
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