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| 1 | IL-17 response mediates acute lung injury induced by the 2009 Pandemic Influenza A (HIN1) Virus显示文摘 | Chenggang Li Penghui Yang Yang Sun Taisheng Li Chen Wang Zhong Wang Zhen Zou Yiwu Yan Wei Wang Chen Wang Zhongwei Chen Li Xing Chong Tang Xiangwu Ju Feng Guo Jiejie Deng Yan Zhao Peng Yang Jun Tang Huanling Wang Zhongpeng Zhao Zhinan Yin Bin Cao Xiliang Wang Chengyu Jiang | 2012 | Cell Research2012，22，3： | 22 |
| 2 | Gastric Lgr5+ stem cells are the cellular origin of invasive intestinal-type gastric cancer in mice显示文摘 | Xiu-Bin Li Guan Yang Liang Zhu Yu-Ling Tang Chong Zhang Zhenyu Ju Xiao Yang Yan Teng | 2016 | Cell Research2016，26，7： | 20 |
| 3 | Investigation of gas content of organic-rich shale:A case study from Lower Permian shale in southern North China Basin,central China显示文摘Measuring gas content is an essential step in estimating the commerciality of gas reserves. In this study,eight shale core samples from the Mouye-1 well were measured using a homemade patented gas desorption apparatus to determine their gas contents. Due to the air contamination that is introduced into the desorption canister, a mathematical method was devised to correct the gas quantity and quality.Compared to the chemical compositions of desorbed gas, the chemical compositions of residual gas are somewhat different. In residual gas, carbon dioxide and nitrogen record a slight increase, and propane is first observed. This phenomenon may be related to the exposure time during the transportation of shale samples from the drilling site to the laboratory, as well as the differences in the mass, size and adsorptivity of different gas molecules. In addition to a series of conventional methods, including the USBM direct method and the Amoco Curve Fit(ACF) method, which were used here for lost gas content estimation, a Modified Curve Fit(MCF) method, based on the 'bidisperse' diffusion model, was established to estimate lost gas content. By fitting the ACF and MCF models to gas desorption data, we determined that the MCF method could reasonably describe the gas desorption data over the entire time period, whereas the ACF method failed. The failure of the ACF method to describe the gas desorption process may be related to its restrictive assumption of a single pore size within shale samples. In comparison to the indirect method, this study demonstrates that none of the three methods studied in this investigation(USBM, ACF and MCF) could individually estimate the lost gas contents of all shale samples and that the proportion of free gas relative to total gas has a significant effect on the estimation accuracy of the selected method. When the ratio of free gas to total gas is lower than 45%, the USBM method is the best for estimating the lost gas content, whereas when the ratio ranges from 45% to 75% or is more than 75%, the ACF and MCF methods, are the best options respectively. | Wei Dang Jin-Chuan Zhang Xuan Tang Xiao-Liang Wei Zhong-Ming Li Cheng-Hu Wang Qian Chen Chong Liu | 2018 | Geoscience Frontiers2018，9，2： | 14 |
| 4 | m^6A-dependent biogenesis of circular RNAs in male germ cells显示文摘The majority of circular RNAs(circRNAs)spliced from coding genes contain open reading frames(ORFs)and thus,have protein coding potential.However,it remains unknown what regulates the biogenesis of these ORF-containing circRNAs,whether they are actually translated into proteins and what functions they play in specific physiological contexts.Here,we report that a large number of circRNAs are synthesized with increasing abundance when late pachytene spermatocytes develop into round and then elongating spermatids during murine spermatogenesis.For a subset of circRNAs,the back splicing appears to occur mostly at m^6A-enriched sites,which are usually located around the start and stop codons in linear mRNAs.Consequently,approximately a half of these male germ cell circRNAs contain large ORFs with m^6A-modified start codons in their junctions,features that have been recently shown to be associated with protein-coding potential.Hundreds of peptides encoded by the junction sequences of these circRNAs were detected using liquid chromatography coupled with mass spectrometry,suggesting that these circRNAs can indeed be translated into proteins in both developing(spermatocytes and spermatids)and mature(spermatozoa)male germ cells.The present study discovered not only a novel role of m^6A in the biogenesis of coding circRNAs,but also a potential mechanism to ensure stable and long-lasting protein production in the absence of linear mRNAs,i.e.,through production of circRNAs containing large ORFs and m^6A-modified start codons in junction sequences. | Chong Tang Yeming Xie Tian Yu Na Liu Zhuqing Wang Rebekah J.Woolsey Yunge Tang Xinzong Zhang Weibing Qin Ying Zhang Ge Song Weiwei Zheng Juan Wang Weitian Chen Xiongyi Wei Zhe Xie Rachel Klukovich Huili Zheng David R.Quilici Wei Yan | 2020 | Cell Research2020，30，3： | 12 |
| 5 | An Operational Test Method for VSC-HVDC Valves Based on Modular Multi-level Converters显示文摘为给基于模块化多电平换流器(modular multi-levelconverter,MMC)的电压源换流器高压直流输电(voltagesourced converter HVDC,VSC-HVDC)换流阀提供有效的检验手段,以验证其设计的正确性及其运行可靠性,对换流阀的运行特性和关键应力进行分析,提出基于原理等效的MMC阀运行试验方法,设计试验电路并阐明其运行机理。搭建的PSCAD电磁暂态仿真模型和实际的试验验证证明了试验方法的正确性和有效性。 | WU Yanan LU Tianguang TANG Guangfu ZHA Kunpeng GAO Chong | 2012 | 中国电机工程学报2012，32，30： | 12 |
| 6 | A unified approach to the weighted Grtzsch and Nitsche problems for mappings of finite distortion显示文摘This note deals with the existence and uniqueness of a minimiser of the following Grtzsch-type problem inf f ∈F∫∫_(Q_1)φ(K(z,f))λ(x)dxdyunder some mild conditions,where F denotes the set of all homeomorphims f with finite linear distortion K(z,f)between two rectangles Q_1 and Q_2 taking vertices into vertices,φ is a positive,increasing and convex function,and λ is a positive weight function.A similar problem of Nitsche-type,which concerns the minimiser of some weighted functional for mappings between two annuli,is also discussed.As by-products,our discussion gives a unified approach to some known results in the literature concerning the weighted Grtzsch and Nitsche problems. | FENG XiaoGao TANG ShuAn WU Chong SHEN YuLiang | 2016 | Science China Mathematics2016，59，4： | 9 |
| 7 | Development and validation of an endoscopic images-based deep learning model for detection with nasopharyngeal malignancies显示文摘Background:Due to the occult anatomic location of the nasopharynx and frequent presence of adenoid hyperpla-sia,the positive rate for malignancy identification during biopsy is low,thus leading to delayed or missed diagnosis for nasopharyngeal malignancies upon initial attempt.Here,we aimed to develop an artificial intelligence tool to detect nasopharyngeal malignancies under endoscopic examination based on deep learning.Methods:An endoscopic images-based nasopharyngeal malignancy detection model(eNPM-DM)consisting of a fully convolutional network based on the inception architecture was developed and fine-tuned using separate training and validation sets for both classification and segmentation.Briefly,a total of 28,966 qualified images were collected.Among these images,27,536 biopsy-proven images from 7951 individuals obtained from January 1st,2008,to December 31st,2016,were split into the training,validation and test sets at a ratio of 7:1:2 using simple randomiza-tion.Additionally,1430 images obtained from January 1st,2017,to March 31st,2017,were used as a prospective test set to compare the performance of the established model against oncologist evaluation.The dice similarity coef-ficient(DSC)was used to evaluate the efficiency of eNPM-DM in automatic segmentation of malignant area from the background of nasopharyngeal endoscopic images,by comparing automatic segmentation with manual segmenta-tion performed by the experts.Results:All images were histopathologically confirmed,and included 5713(19.7%)normal control,19,107(66.0%)nasopharyngeal carcinoma(NPC),335(1.2%)NPC and 3811(13.2%)benign diseases.The eNPM-DM attained an overall accuracy of 88.7%(95%confidence interval(CI)87.8%-89.5%)in detecting malignancies in the test set.In the prospective comparison phase,eNPM-DM outperformed the experts:the overall accuracy was 88.0%(95%CI 86.1%-89.6%)vs.80.5%(95%CI 77.0%-84.0%).The eNPM-DM required less time(40 s vs.110.0±5.8 min)and exhibited encouraging performance in automatic segmentation of nasopharyngeal malignant area from the background,with an average DSC of 0.78±0.24 and 0.75±0.26 in the test and prospective test sets,respectively.Conclusions:The eNPM-DM outperformed oncologist evaluation in diagnostic classification of nasopharyngeal mass into benign versus malignant,and realized automatic segmentation of malignant area from the background of nasopharyngeal endoscopic images. | Chaofeng Li Bingzhong Jing Liangru Ke Bin Li Weixiong Xia Caisheng He Chaonan Qian Chong Zhao Haiqiang Mai Mingyuan Chen Kajia Cao Haoyuan Mo Ling Guo Qiuyan Chen Linquan Tang Wenze Qiu Yahui Yu Hu Liang Xinjun Huang Guoying Liu Wangzhong Li Lin Wang Rui Sun Xiong Zou Shanshan Guo Peiyu Huang Donghua Luo Fang Qiu Yishan Wu Yijun Hua Kuiyuan Liu Shuhui Lv Jingjing Miao Yanqun Xiang Ying Sun Xiang Guo Xing Lv | 2018 | Cancer Communications2018，38，1： | 9 |
| 8 | N400 elicited by incongruent ending words of Chinese idioms in healthy adults显示文摘关于 N400 的背景优先的研究主要基于英语刺激,当汉字的 cognitive.processing 仍然是礼品的 unclear.The 目的时,学习是进一步调查中国 idioms.Methods 事件相关潜力( ERP )的语义处理部件 N400 被 38 匹配得到(适合)并且错配(不一致)有一样的音素的结束中国 idioms:ending 词但是不同形状和意思( sPdSdM ),与类似的形状但是不同 | CHEN Xing-shi TANG Yun-xiang XIAO Ze-ping WANG Ji-jun ZHANG Ming-dao ZHANG Zai-fu HU Zhen-yu LOU Fei-ying CHEN Chong ZHANG Tian-hong | 2010 | Chinese Medical Journal2010，，6： | 8 |
| 9 | Cryogenic 3D printing of dual-delivery scaffolds for improved bone regeneration with enhanced vascularization显示文摘Three-dimensional(3D)printing has been increasingly employed to produce advanced bone tissue engineering scaffolds with biomimetic structures and matched mechanical strengths,in order to induce improved bone regeneration in defects with a critical size.Given that the successful bone regeneration requires both excellent osteogenesis and vascularization,endowing scaffolds with both strong bone forming ability and favorable angiogenic potential would be highly desirable to induce improved bone regeneration with required vascularization.In this investigation,customized bone tissue engineering scaffolds with balanced osteoconductivity/osteoinductivity were produced via cryogenic 3D printing ofβ-tricalcium phosphate and osteogenic peptide(OP)containing water/poly(lactic-co-glycolic acid)/dichloromethane emulsion inks.The fabricated scaffolds had a hierarchically porous structure and were mechanically comparable to human cancellous bone.Angiogenic peptide(AP)containing collagen I hydrogel was then coated on scaffold surface to further provide scaffolds with angiogenic capability.A sequential release with a quick AP release and a slow but sustained OP release was obtained for the scaffolds.Both rat endothelial cells(ECs)and rat bone marrow derived mesenchymal stem cells(MSCs)showed high viability on scaffolds.Improved in vitro migration and angiogenesis of ECs were obtained for scaffolds delivered with AP while enhanced osteogenic differentiation was observed in scaffolds containing OP.The in vivo results showed that,toward scaffolds containing both AP and OP,the quick release of AP induced obvious angiogenesis in vivo,while the sustained OP release significantly improved the new bone formation.This study provides a facile method to produce dual-delivery scaffolds to achieve multiple functions. | Chong Wang Jiahui Lai Kai Li Shaokui Zhu Bingheng Lu Jia Liu Yujin Tang Yen Wei | 2021 | Bioactive Materials2021，6，1： | 7 |
| 10 | Apelin-13 inhibits lipoprotein lipase expression via the APJ/PKCα/miR-361-5p signaling pathway in THP-1 macrophage-derived foam cells显示文摘动脉粥样硬化患者损害被丰富的类脂化合物和长期的发炎的累积描绘。以前的研究显示了导出巨噬细胞的脂蛋白脂肪分解酵素(LPL ) 由加速类脂化合物累积和支持 inflammatory cytokine 分泌物支持动脉粥样硬化前进。尽管 apelin-13 被认为是一个 atheroprotective 因素,它是否能调整 LPL 的表示,仍然保持不清楚。这研究的目的是在 THP-1 导出巨噬细胞的泡沫房间在 LPL 和内在的机制的表示上探索 apelin-13 的效果。Apelin-13 显著地减少了在 10 和 100 nM 的集中的全部的胆固醇,免费胆固醇,和胆固醇酉旨的细胞的层次。ELISA 分析证实有 apelin-13 的处理减少了支持 inflammatory cytokine 分泌物,例如 interleukin-6 (IL-6 ) , interleukin-1 (IL-1 ) 和肿瘤坏死 factor-alpha (TNF-) 。apelin-13 禁止了由西方的污点和即时 PCR 分析揭示了的 LPL 的表示,这也被发现。生物信息学分析和双酶的记者试金直接显示了那 miR-361-5p downregulated 由指向 LPL 的 3UTR 的 LPL 的表示。另外, apelin-13 + miR-361-5p 显著地模仿 downregulated 在房间的 LPL 的表示。最后,我们表明了那 apelin-13 downregulated 通过激活 PKC 的活动的 LPL 的表示。一起拿,我们的结果显示出那 apelin-13 downregulated 经由激活在 THP-1 导出巨噬细胞的泡沫房间表明小径的 APJ/PKC/miR-361-5p 的 LPL 的表示,导致类脂化合物累积和支持 inflammatory cytokine 分泌物的抑制。因此,我们的研究由 apelin-13 提供重要新卓见进类脂化合物累积和支持 inflammatory cytokine 分泌物的抑制,并且在动脉粥样硬化作为一个有希望的治疗学的目标加亮 apelin-13。 | Xin Zhang Qiong Ye Duo Gong Yuan Lv Haipeng Cheng Chong Huang Lingyan Chen Zhenwang Zhao Liang Li Xie Wei Min Zhang Xiaodan Xia Xiaohua Yu Xilong Zheng Shuzhi Wang Zongbao Wang Chaoke Tang | 2017 | Acta Biochimica et Biophysica Sinica2017，49，6： | 6 |
| 11 | IL-17 sustains the plasma cell response via p38-mediated Bcl-xL RNA stability in lupus pathogenesis显示文摘Recent studies have demonstrated a central role for plasma cells in the development of autoimmune diseases,such as systemic lupus erythematosus(SLE).Currently,both the phenotypic features and functional regulation of autoreactive plasma cells during SLE pathogenesis remain largely unclear.In this study,we first found that a major subset of IL-17 receptor-expressing plasma cells potently produced anti-dsDNA IgG upon IL-17A(IL-17)stimulation in SLE patients and lupus mice.Using a humanized lupus mouse model,we showed that the transfer of Th17 cell-depleted PBMCs from lupus patients resulted in a significantly reduced plasma cell response and attenuated renal damage in recipient mice compared to the transfer of total SLE PBMCs.Moreover,long-term BrdU incorporation in lupus mice detected highly enriched long-lived BrdU+subsets among IL-17 receptor-expressing plasma cells.Lupus mice deficient in IL-17 or IL-17 receptor C(IL-17RC)exhibited a diminished plasma cell response and reduced autoantibody production with attenuated renal damage,while the adoptive transfer of Th17 cells triggered the plasma cell response and renal damage in IL-17-deficient lupus mice.In reconstituted chimeric mice,IL-17RC deficiency resulted in severely impaired plasma cell generation but showed no obvious effect on germinal center B cells.Further mechanistic studies revealed that IL-17 significantly promoted plasma cell survival via p38-mediated Bcl-xL transcript stabilization.Together,our findings identified a novel function of IL-17 in enhancing plasma cell survival for autoantibody production in lupus pathogenesis,which may provide new therapeutic strategies for the treatment of SLE. | Kongyang Ma Wenhan Du Fan Xiao Man Han Enyu Huang Na Peng Yuan Tang Chong Deng Lixiong Liu Yulan Chen Jingjing Li Shiwen Yuan Qin Huang Xiaoping Hong Dajun Hu Xiaoyan Cai Quan Jiang Dongzhou Liu Liwei Lu | 2021 | Cellular & Molecular Immunology2021，18，7： | 6 |
| 12 | Differences in P50 and prepulse inhibition of the startle reflex between male smokers and non-smokers with first episode schizophrenia without medical treatment显示文摘 | Song Lisheng Chen Xingshi Chen Meijuan Tang Yunxiang Wang Jijun Zhang Mingdao Lou Feiying Liang Jianhua Chen Chong | 2014 | Chinese Medical Journal2014，，9： | 6 |
| 13 | Macrophage-activating lipopeptide-2 downregulates the expression of ATP-binding cassette transporter A1 by activating the TLR2/ NF-KB/ZNF202 pathway in THP-1 macrophages显示文摘 | Liangjie Peng Zizhen Zhang Min Zhang Xiaohua Yu Feng Yao Yulin Tan Dan Liu Duo Gong Huang Chong Xiaoyan Liu Xilong Zheng Guoping Tian Chaoke Tang | 2016 | Acta Biochimica et Biophysica Sinica2016，48，4： | 6 |
| 14 | Application of ultrasound contrast in identification and diagnosis of ocular spaceoccupying lesions显示文摘AIM: To analyze the application significance of ultrasound contrast agent in identification and diagnosis of ocular spaceoccupying lesions, and mainly analyze its advantages and problems. METHODS: Thirty-two representative literatures about the application of ultrasound contrast agent in diagnosis of spaceoccupying lesions at home and abroad were collected after focused on sorting the literature reporting the application of ultrasound contrast diagnostic technology in the diagnosis and identification of ocular spaceoccupying lesions in recent years. Its advantages and problems were retrospectively analyzed, and reasonable assessment on existing problems was made and possible solutions to the problems were proposed. RESULTS: As a new imaging diagnostic technique, the contrast-enhanced ultrasound, which can enhance the display of tumor microcirculation vessels and improve the tumor's ultrasound diagnostic capability, was analyzed. Through sorting and comprehensively analyzing the collected literatures, the positive rate of ocular spaceoccupying lesion diagnosis could be significantly improved with ultrasound contrast technology. Thus, the vascular perfusion in normal tissues and lesions was reflected objectively. According to the lesion's perfusion characteristics of the contrast agent plus with the performance features of two-dimensional ultrasound, the ocular spaceoccupying lesions can be accurately diagnosed, and this could provide clinicians with reliable research basis in this field. CONCLUSION: Ultrasound contrast examination is a new testing method, and ultrasound contrast agent can significantly enhance the ultrasonic detection signal, clearly show the blood perfusion in vessels and tissues, increase the image contrast resolution, and improve the lesion's detection capability in the microcirculation perfusion level, especially its important value in the diagnosis of ocular tumor. | Jia-Ying Yuan, Yun-Tao Zhang | 2011 | International Journal of Ophthalmology(English edition)2011，4，4： | 5 |
| 15 | An all-round AI-Chemist with a scientific mind显示文摘The realization of automated chemical experiments by robots unveiled the prelude to an artificial intelligence(AI)laboratory.Several AI-based systems or robots with specific chemical skills have been demonstrated,but conducting all-round scientific research remains challenging.Here,we present an all-round AI-Chemist equipped with scientific data intelligence that is capable of performing basic tasks generally required in chemical research.Based on a service platform,the AI-Chemist is able to automatically read the literatures from a cloud database and propose experimental plans accordingly.It can control a mobile robot in-house or online to automatically execute the complete experimental process on 14 workstations,including synthesis,characterization and performance tests.The experimental data can be simultaneously analysed by the computational brain of the AI-Chemist through machine learning and Bayesian optimization,allowing a new hypothesis for the next iteration to be proposed.The competence of the AI-Chemist has been scrutinized by three different chemical tasks.In the future,the more advanced all-round AI-Chemists equipped with scientific data intelligence may cause changes to the landscape of the chemical laboratory. | Qing Zhu Fei Zhang Yan Huang Hengyu Xiao LuYuan Zhao XuChun Zhang Tao Song XinSheng Tang Xiang Li Guo He BaoChen Chong JunYi Zhou YiHan Zhang Baicheng Zhang JiaQi Cao Man Luo Song Wang GuiLin Ye WanJun Zhang Xin Chen Shuang Cong Donglai Zhou Huirong Li Jialei Li Gang Zou WeiWei Shang Jun Jiang Yi Luo | 2022 | National Science Review2022，9，10： | 5 |
| 16 | Safety, pharmacokinetics, and biomarkers of F-652, a recombinant human interleukin-22 dimer, in healthy subjects显示文摘F-652 is a recombinant fusion protein consisting of two human interleukin-22(IL-22)molecules linked to an immunoglobulin constant region(IgG 2-Fc).IL-22 plays critical roles in promoting tissue repair and suppressing bacterial infection.The safety,pharmacokinetics(PK),tolerability,and biomarkers of F-652 were evaluated following a single dose in healthy male volunteers in a randomized,double-blind,placebo-controlled study.Following single-dose subcutaneous(SC)injection of F-652 at 2.0µg/kg into healthy subjects,six out of six subjects experienced delayed injection site reactions,which presented as erythematous and/or discoid eczematous lesions 10 to 17 days post-dosing.F-652 was then administered to the healthy subjects via an intravenous(IV)infusion at 2.0,10,30,and 45µg/kg.No severe adverse event(SAE)was observed during the study.Among the IV-dosed cohorts,eye and skin treatment emergent adverse events(TEAEs)were observed in the 30 and 45µg/kg cohorts.F-652 IV dosing resulted in linear increases in C max and AUC(0–t),and the T 1/2 ranged from 39.4 to 206h in the cohorts.An IV injection of F-652 induced dose-dependent increases in serum marker serum amyloid A,C-reactive protein,and FIB,and decreased serum triglycerides.The serum levels of 36 common pro-inflammatory cytokines/chemokines were not altered by the treatment of F-652 at 45μg/kg.In conclusion,IV administration of F-652 to healthy male volunteers is safe and well-tolerated and demonstrates favorable PK and pharmacodynamic properties.These results warrant further clinical development of F-652 to treat inflammatory diseases. | Kai-Yang Tang Jason Lickliter Zhi-Hua Huang Zong-Shu Xian Han-Yang Chen Cheng Huang Chong Xiao Yu-Peng Wang Ying Tan Lin-Feng Xu Yu-Liang Huang Xiao-Qiang Yan | 2019 | Cellular & Molecular Immunology2019，16，5： | 5 |
| 17 | Neonatal ventral hippocampal lesion as a valid model of schizophrenia: evidence from sensory gating study显示文摘 | CHEN Xing-shi ZHANG Chen XU Yi-feng ZHANG Ming-dao LOU Fei-ying CHEN Chong TANG Jin | 2012 | Chinese Medical Journal2012，，15： | 5 |
| 18 | Key stress extraction and equivalent test method for hybrid DC circuit breaker显示文摘Firstly, relevant stress properties of millisecond level breaking process and microsecond level commutation process of hybrid HVDC circuit breaker are studied in detail on the basis of the analysis for the application environment and topological structure and operating principles of hybrid circuit breakers, and key stress parameters in transient state process of two time dimensions are extracted. The established digital simulation circuit for PSCAD/EMTDC device-level operation of the circuit breaker has verified the stress properties of millisecond level breaking process and microsecond level commutation process. Then, equivalent test method, circuits and parameters based on LC power supply are proposed on the basis of stress extraction. Finally, the results of implemented breaking tests for complete 200 kV circuit breaker, 100 kV and 50 kV circuit breaker units, as well as single power electronic module have verified the accuracy of the simulation circuit and mathematical analysis. The result of this paper can be a guide to electrical structure and test system design of hybrid HVDC circuit breaker. | Chong Gao Xiao Ding Guangfu Tang Gaoyong Wang Peng Qiu | 2018 | Global Energy Interconnection2018，1，1： | 4 |
| 19 | Celecoxib attenuates hepatocyte apoptosis by inhibiting endoplasmic reticulum stress in thioacetamide-induced cirrhotic rats显示文摘BACKGROUND Endoplasmic reticulum(ER)stress is an important mechanism in the progression of chronic and acute liver diseases,especially in the progression and recovery of liver fibrosis.Excessive and long-term ER stress induces apoptosis.ER stressinduced apoptosis is considered to be an important pathway in the development of liver fibrosis.Cyclooxygenase-2(COX-2)induction is also closely related to ER stress.In our previous studies,we showed that celecoxib,a COX-2 inhibitor,improves liver fibrosis and portal hypertension.However,the role and mechanism of celecoxib in alleviating liver fibrosis remain unclear.AIM To investigate whether celecoxib alleviates liver fibrosis by inhibiting hepatocyte apoptosis via the ER stress response.METHODS Cirrhosis was induced by intraperitoneal injections of thioacetamide(TAA)for 16 wk(injection dose is 200 mg/kg per 3 d for the first 8 wk and 100 mg/kg per 3 d after 8 wk).Thirty-six male Sprague-Dawley rats were randomly divided into three groups,namely,control group,TAA group,and TAA+celecoxib group.In the last 8 wk,TAA-induced cirrhotic rats received celecoxib(20 mg/kg/day)or the vehicle by gastric gavage.After 16 wk,the rats were sacrificed,and serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),and albumin(ALB)were detected.The hepatic fibrosis areas were evaluated by Sirius red staining and the degree of fibrosis was assessed by measuring the level of hydroxyproline.ER stress levels were evaluated by detecting the marker proteins glucose-regulated protein 78(GRP78),CCAAT/enhancer binding protein homologous protein(CHOP),PKR-like ER protein kinase(PERK),activating transcription factor 6(ATF6),and inositol-requiring enzyme 1 alpha(IRE1α).Apoptosis levels were evaluated by detecting caspase-12 and caspase-3.RESULTS The serum ALT and AST levels in the liver were significantly reduced by celecoxib;however,the serum ALB had no significant changes.Celecoxib significantly reduced the degree of liver fibrosis and the levels of hydroxyproline(-38%and-25.7%,respectively,P<0.01).Celecoxib ameliorated ER stress by reducing the level of GRP78 compared to the TAA group(P<0.05).Consistently,after celecoxib administration,the upregulation of TAA-induced hepatic apoptosis markers(caspase-12 and caspase-3)and CHOP were significantly inhibited.In addition,after celecoxib treatment,the expression of key molecules associated with ER stress(PERK,ATF6,and IRE1)was decreased(P<0.05).CONCLUSION Therapeutic administration of celecoxib effectively reduces hepatic apoptosis in TAA-induced cirrhotic rats.The mechanism of action may be attributed to the suppression of CHOP expression,which subsequently inhibits ER stress. | Wei Su Yang Tai Shi-Hang Tang Yan-Ting Ye Chong Zhao Jin-Hang Gao Bi-Guang Tuo Cheng-Wei Tang | 2020 | World Journal of Gastroenterology2020，26，28： | 4 |
| 20 | The Monte-Carlo simulation on a scintillator neutron detector显示文摘A simulation of the properties of the shifting scintillator neutron detector using 6LiF/ZnS(Ag) scintillation screens is performed.The simulation results show that the light attenuation length of standard BC704 scintillator is about 0.65 mm.Its thermal neutron detection efficiency,gamma sensitivity and intrinsic spatial resolution can achieve around 50.0%,10 5and 0.18 mm(along X-axis) respectively.For the detector,air coupling position resolution is better than the silicone oil coupling.Some of the simulation results are compared with experimental results.They are in agreement.This work will be helpful for constructing neutron detector for high intensity powder diffractometer at Chinese spallation neutron source. | WU Chong TANG Bin SUN ZhiJia ZHANG Qiang YANG Zhen LUO Wei WANG Tuo | 2013 | Science China(Physics,Mechanics & Astronomy)2013，56，10： | 4 |
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