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| 1 | 日本精确农业的研究现状显示文摘精确农业作为基于知识的农业技术体系,被认为是实现农业可持续发展的先进生产方式。本文介绍了日本在精确农业领域的研究现状,并针对我国的精确农业研究提出几点思考与建议。 | YANG Yinsheng Wu Caicong Feng Chuanping 杨印生 吴才聪 冯传平 | 2001 | 农业机械学报2001，32，2： | 21 |
| 2 | Thermal energy storage:Challenges and the role of particle technology显示文摘Thermal energy is at the heart of the whole energy chain providing a main linkage between the primary and secondary energy sources.Thermal energy storage(TES)has a pivotal role to play in the energy chain and hence in future low carbon economy.However,a competitive TES technology requires a number of scientific and technological challenges to be addressed including TES materials,TES components and devices,and integration of TES devices with energy networks and associated dynamic optimization.This paper provides a perspective of TES technology with a focus on TES materials challenges using molten salts based phase change materials for medium and high temperature applications.Two key challenges for the molten salt based TES materials are chemical incompatibility and low thermal conductivity.The use of composite materials provides an avenue to meeting the challenges.Such composite materials consist of a phase change material,a structural supporting material,and a thermal conductivity enhancement material.The properties of the supporting material could determine the dispersion of the thermal conductivity enhancement material in the salt.A right combination of the salt,the structural supporting material,and the thermal conductivity enhancement material could give a hierarchical structure that is able to encapsulate the molten salt and give a substantial enhancement in the thermal conductivity.Understanding of the structure-property relationships for the composite is essential for the formulation design and fabrication of the composite materials.Linking materials properties to the system level performance is recommended as a key future direction of research. | Zhiwei Ge Yongliang Li Dacheng Li Ze Sun Yi Jin Chuanping Liu Chuan Li Guanghui Leng Yulong Ding | 2014 | Particuology2014，12，4： | 20 |
| 3 | Humoral immune response to circulating SARS-CoV-2 variants elicited by inactivated and RBD-subunit vaccines显示文摘SARS-CoV-2 variants could induce immune escape by mutations on the receptor-binding domain(RBD)and N-terminal domain(NTD).Here we report the humoral immune response to circulating SARS-CoV-2 variants,such as 501Y.V2(B.1.351),of the plasma and neutralizing antibodies(NAbs)elicited by CoronaVac(inactivated vaccine),ZF2001(RBD-subunit vaccine)and natural infection.Among 86 potent NAbs identified by high-throughput single-cell VDJ sequencing of peripheral blood mononuclear cells from vaccinees and convalescents,near half anti-RBD NAbs showed major neutralization reductions against the K417N/E484K/N501Y mutation combination,with E484K being the dominant cause.VH3-53/VH3-66 recurrent antibodies respond differently to RBD variants,and K417N compromises the majority of neutralizing activity through reduced polar contacts with complementarity determining regions.In contrast,the 242–244 deletion(242–244Δ)would abolish most neutralization activity of anti-NTD NAbs by interrupting the conformation of NTD antigenic supersite,indicating a much less diversity of anti-NTD NAbs than anti-RBD NAbs.Plasma of convalescents and CoronaVac vaccinees displayed comparable neutralization reductions against pseudo-and authentic 501Y.V2 variants,mainly caused by E484K/N501Y and 242–244Δ,with the effects being additive.Importantly,RBD-subunit vaccinees exhibit markedly higher tolerance to 501Y.V2 than convalescents,since the elicited anti-RBD NAbs display a high diversity and are unaffected by NTD mutations.Moreover,an extended gap between the third and second doses of ZF2001 leads to better neutralizing activity and tolerance to 501Y.V2 than the standard three-dose administration.Together,these results suggest that the deployment of RBD-vaccines,through a third-dose boost,may be ideal for combating SARS-CoV-2 variants when necessary,especially for those carrying mutations that disrupt the NTD supersite. | Yunlong Cao Ayijiang Yisimayi Yali Bai Weijin Huang Xiaofeng Li Zhiying Zhang Tianjiao Yuan Ran An Jing Wang Tianhe Xiao Shuo Du Wenping Ma Liyang Song Yongzheng Li Xiang Li Weiliang Song Jiajing Wu Shuo Liu Xuemei Li Yonghong Zhang Bin Su Xianghua Guo Yangyang Wei Chuanping Gao Nana Zhang Yifei Zhang Yang Dou Xiaoyu Xu Rui Shi Bai Lu Ronghua Jin Yingmin Ma Chengfeng Qin Youchun Wang Yingmei Feng Junyu Xiao Xiaoliang Sunney Xie | 2021 | Cell Research2021，31，7： | 12 |
| 4 | Identification of novel cis-elements bound by BplMYB46 involved in abiotic stress responses and secondary wall deposition显示文摘Transcription factors(TFs)play vital roles in various biological processes by binding to cis-acting elements to control expressions of their target genes.The MYB TF BplMYB46,from Betula platyphylla,is involved in abiotic stress responses and secondary wall deposition.In the present study,we used a TF-centered yeast onehybrid technology(TF-centered Y1H)to identify the cisacting elements bound by BplMYB46.We screened a shortinsert random library and identified three cis-elements bound by BplMYB46:an E-box(CA(A/T/C)(A/G/C)TG)and two novel motifs,a TC-box(T(G/A)TCG(C/G))and a GT-box(A(G/T)T(A/C)GT(T/G)C).Chromatin immunoprecipitation(ChIP)and effector-reporter coexpression assays in Nicotiana tabacum confirmed binding of BplMYB46 to the TC-box,GT-box,and E-box motifs in the promoters of the phenylalanine ammonia lyase(PAL),peroxidase(POD),and superoxide dismutase(SOD)genes,which function in abiotic stress tolerance and secondary wall biosynthesis.This finding improves our understanding of potential regulatory mechanisms in the response to abiotic stress and secondary wall deposition of BplMYB46 in B.platyphylla. | Huiyan Guo Liuqiang Wang Chuanping Yang Yiming Zhang Chunrui Zhang Chao Wang | 2018 | Journal of Integrative Plant Biology2018，60，10： | 7 |
| 5 | Allocation Methodology of Multiple SVCs Considering Operation Scheme Changing显示文摘针对多静止同步补偿器(staticvarcompensator,SVC)对系统共同影响的问题,提出计及运行方式变化的多SVC布点方法。利用规范形分析,基于摄动方程2阶解析解信息建立拓展静态电压稳定边界的多SVC布点分析方法。首先根据静态电压稳定分析方法,确定系统危险运行方式(即距离系统电压崩溃最近的运行方式1;然后在该运行方式下,利用规范形方法对系统进行小扰动电压稳定分析,求出所有负荷节点的电压振荡曲线解析解;最后利用2阶解析解所给出的小扰动1阶振荡幅值和2阶振荡幅值信息,以多SVC布点多种方案对系统降低小扰动幅值的贡献为指标,给出多SVC布点结果。所提方法应用于IEEE30节点系统,试验结果表明,该方法相对于不考虑多点共同影响的多SVC布点方式,其静态电压稳定边界的拓展效果更好。 | XIONG Haoqing TANG Yong ZHANG Xiaohua MENG Yuanjing LIU Yuexin FU Hongjun SUN Huadong YI Jun XIONG Chuanping | 2012 | 中国电机工程学报2012，32，13： | 6 |
| 6 | Clonal variations in nutritional components of Pinus koreansis seeds collected from seed orchards in Northeastern China显示文摘From four Korean pine(Pinus koraiensis)orchards, 60 clones were selected and analyzed for the fatty acid and amino acid components of the seeds to reveal the variations and correlations of the seed characteristics among the clonal source orchards and clones. The nutritional components of the seeds of the P. koraiensis trees exhibited rich genetic variation; the variation coefficient of the fatty acids was 2.24–66.83 %, while the variation coefficient of the amino acids was 14.70–38.88 %. Relatively high genetic-improvement potential exists for the nutritional components of the seeds. The phenotypic differentiation of the fatty acid and amino acid components reveals that variation within the population(85.18 %) was the primary source for the variation of the fatty acid components; variation among the orchards(63.08 %) was the primary source of the variation of the amino acid components. Data drawn from various clonal source orchards all showed that the seed characteristics were highly controlled by heritability(h2[ 80 %), and the seed characteristics of the P. koraiensis trees exhibited a similar genetic gain trend. The principal components were analyzed to obtain the comprehensive principal component value for each clonal seed orchard. Twelve clones were selected based on a clonal selection rate of 20 %. Correlation and multiple stepwise-regression analyses were conducted, considering different location conditions, to reveal the stable correlations between the seed characteristics to facilitate improvements of the seed yield of P.koraiensis trees and the clonal selection. Species of real characteristics in P. koraiensis were controlled by higher heritability. Genetic gain was obtained by selecting of superior clones. | Zhen Zhang Hanguo Zhang Chuanping Yang Lei Zhang Jia Du Ying Jiang | 2016 | Journal of Forestry Research2016，27，2： | 6 |
| 7 | The applicability of biochar and zero-valent iron for the mitigation of arsenic and cadmium contamination in an alkaline paddy soil显示文摘In paddy fields, the opposing transformation of arsenic (As) and cadmium (Cd) poses many challenges for their simultaneous remediation.In our previous study,we reported that combined biochar and zero-valent iron(ZVI)amendment had great potential for the simultaneous alleviation of As and Cd bioavailability in contaminated acid paddy soil.In this study,an As-and Cd-contaminated alkaline paddy soil was further studied,and the same ZVI-biochar mixtures amendments were applied to evaluate the impact of the mixtures on As and Cd transformation and translocation in the soil-rice system by performing pot experiments with rice.In line with our previous study,the ZVI-biochar composites significantly reduced As and Cd accumulation in different rice tissues,leading to a 42%and 47%decrease in rice grain As and Cd levels,respectively,compared with the control values.The ZVI-biochar mixtures exhibited synergistic effects of biochar and ZVI by enhancing the transformation of bioavailable As and Cd fractions into less bioavailable fractions,and by increasing iron plaque formation to reduce As and Cd bioavailability.Although the bioaccumulation and translocation factors of As and Cd in alkaline paddy soil were generally lower than those in acid paddy soil,particularly in the presence of the ZVI-biochar mixtures,the grain As and Cd levels did not achieve the desired food safety standard levels,probably related to the high soil As content and the small changes in soil pH.Nevertheless,for treating lightly and moderately contaminated paddy soils,ZVI-biochar mixtures can still be a good choice in the future. | Jiangtao Qiao Huanyun Yu Xiangqin Wang Fangbai Li Qi Wang Yuzhen Yuan Chuanping Liu | 2019 | Biochar2019，1，2： | 5 |
| 8 | Recent advances in fermentative biohydrogen production显示文摘Hydrogen energy, as a kind of clean energy with great potential, has been a hotspot for study worldwide. Based on the recent research on biohydrogen production, this paper gives a brief review on the following aspects: fermentative hydrogen production process and the engineering control statagy, key factors affecting the effciency of hydrogen production, such as substrates, cysteine, metal ions, anaerobic fermentation terminal products, and formic acid and ammonia. Moreover, anaerobic fermentative hydrogen-producing strain and reg- ulation and control of enzyme gene in fermentative hydrogen production are also discussed. Finally, the prospect of anaerobic fermen- tative biohydrogen production is proposed in three study areas, namely developing new techniques for breeding hydrogen-producing bacteria, exploitations of more strains and gene resources, and intensifying the application of microbial molecular breeding in hydrogen production. | Xuemei Liu Nanqi Ren Funan Song Chuanping Yang Aijie Wang | 2008 | Progress in Natural Science:Materials International2008，18，3： | 4 |
| 9 | Research progress in electrospinning engineering for all-solid-state electrolytes of lithium metal batteries显示文摘Owing to safety issue and low energy density of liquid lithium-ion batteries(LIBs),all-solid-state lithium metal batteries(ASLMBs)with unique all-solid-state electrolytes(SEs)have attracted wide attentions.This arises mainly from the advantages of the SEs in the suppression of lithium dendrite growth,long cycle life,and broad working temperature range,showing huge potential applications in electronic devices,electric vehicles,smart grids,and biomedical devices.However,SEs suffer from low lithiumion conductivity and low mechanical integrity,slowing down the development of practical ASLMBs.Nanostructure engineering is of great efficiency in tuning the structure and composition of the SEs with improved lithium-ion conductivity and mechanical integrity.Among various available technologies for nanostructure engineering,electrospinning is a promising technique because of its simple operation,cost-effectiveness,and efficient integration with different components.In this review,we will first give a simple description of the electrospinning process.Then,the use of electrospinning technique in the synthesis of various SEs is summarized,for example,organic nanofibrous matrix,organic/inorganic nanofibrous matrix,and inorganic nanofibrous matrix combined with other components.The current development of the advanced architectures of SEs through electrospinning technology is also presented to provide references and ideas for designing high-performance ASLMBs.Finally,an outlook and further challenges in the preparation of advanced SEs for ASLMBs through electrospinning engineering are given. | Manxi Wang Yaling Wu Min Qiu Xuan Li Chuanping Li Ruiling Li Jiabo He Ganggang Lin Qingrong Qian Zhenhai Wen Xiaoyan Li Ziqiang Wang Qi Chen Qinghua Chen Jinhyuk Lee Yiu-Wing Mai Yuming Chen | 2021 | Journal of Energy Chemistry2021，30，10： | 4 |
| 10 | Analysis of three types of triterpenoids in tetraploid white birches(Betula platyphylla Suk.) and selection of plus trees显示文摘Betulin, oleanolic acid, and betulinic acid are naturally occurring pentacyclic triterpenoids that have significant medicinal value. Considerable amounts of these triterpenoids are available in the outer bark of white birch.In this study, we used ultrasound-assisted extraction(UAE)to extract triterpenoids from birch bark rapidly and with high efficiency. Using high performance liquid chromatography(HPLC), three types of triterpenoids were separated and detected. We examined the differences among triterpenoids extracted from diploid versus tetraploid white birch. Then, we used factor analysis to screen out tetraploid white birches with comprehensively excellent performance. The results indicate that the optimum conditions for extraction include the use of ethanol as an extraction solvent, a solid-to-liquid ratio of 0.1 g/10 ml,ultrasonic power set at 100 W, a temperature of 60 °C and an extraction time of 15 min. A reversed-phase C18 column(4.6 mm × 250 mm × 5 lm) with a column temperature of 30 °C and the mobile phase composed of A(acetonitrile) and B(0.1 % aqueous phosphoric acid, v/v)at a flow rate of 0.5 ml/min were used, and the detection wavelength was 195 nm. No significant difference was observed between diploid and tetraploid white birch in terms of the content of three types of triterpenoids(at a confidence level of 0.05). As triterpenoid content, height,and DBH(diameter at breast height) are strongly interrelated, we used factor analysis to evaluate all individuals,and we screened out six plus trees with excellent comprehensive characters. | Sui Wang Hui Zhao Jing Jiang Guifeng Liu Chuanping Yang | 2015 | Journal of Forestry Research2015，26，3： | 4 |
| 11 | Tribological and tribochemical properties of magnetite nanoflakes as additives in oil lubricants显示文摘This detailed the tribological and tribochemical properties of magnetite(Fe_3O_4) nanoflakes used as additives in #40 base oil in a four-ball tribo-tester.The average friction coefficient of the friction pair for lubricant containing the Fe_3O_4 nanoflakes of 1.5 wt%as a lubricant additive in the base oil is decreased by18.06%compared to that of solely base oil.The chemical composition of base oil with the Fe_3O_4 nanoflake additives did not change during the 48-h friction assessment.The decreased saturation magnetization and increased coercivity of magnetite nanoflakes occurred due to the distortion of the basal planes and the presence of hematite(α-Fe_3O_4) generated by the tribochemical reactions during the friction process.The multi-layer low-shear-stress tribochemical lubrication films on the surface of the friction pair could form because the nanoflake particles arrange and adhere onto the surface of the friction pair in an orderly manner,and the tribochemical reactions of the friction pair in the presence of the nanoflakes occur as Fe→FeO→Fe_3O_4→γ-FeOOH →γ-Fe_2O_3→α-Fe_2O_3.The formation of the films can improve the tribological properties. | Longhua Xiang Chuanping Gao Yanmin Wang Zhidong Pan Dawei Hu | 2014 | Particuology2014，12，6： | 4 |
| 12 | Two novel eukaryotic translation initiation factor 5A genes from Populus simonii×P.nigra confer tolerance to abiotic stresses in Saccharomyces cerevisiae显示文摘The role of plant e IF5 A proteins in multiple biological processes, such as protein synthesis regulation,translation elongation, m RNA turnover, programmed cell death and stress tolerance is well known. Toward using these powerful proteins to increase stress tolerance in agricultural plants, in the present study, we cloned and characterized Psne IF5A2 and Psne IF5A4 from young poplar(P. simonii 9 P. nigra) leaves. The deduced amino acid sequences of Psne IF5A2 and Psne IF5A4 were 98 %similar to each other, and they are orthologs of e IF5A1 in Arabidopsis. In a subcellular localization analysis,Psne IF5A2 and Psne IF5A4 proteins were localized in the nucleus and cytoplasm. q RT-PCR analysis showed that Psne IF5A2 and Psne IF5A4 were transcribed in poplar flowers, stem, leaves, and roots. In addition, they were also induced by abiotic stresses. Transgenic yeast expressing Psne IF5A2 and Psne IF5A4 had increased salt, heavy metal, osmotic, oxidative tolerance. Our results suggest that Psne IF5A2 and Psne IF5A4 are excellent candidates for genetic engineering to improve salt and heavy metal tolerance in agricultural plants. | Tangchun Zheng Lina Zang Lijuan Dai Chuanping Yang Guanzheng Qu | 2017 | Journal of Forestry Research2017，28，3： | 4 |
| 13 | Bifunctional plasmonic colloidosome/graphene oxidebased floating membranes for recyclable high-efficiency solar-driven clean water generation显示文摘为太阳的水蒸发利用 plasmonic nano-particles/structures 引起了增加的兴趣;然而,大规模方法被需要增加效率并且改进太阳的蒸气产生的实物。我们基于 graphene 氧化物和多尺度的 plasmonic nanostructure 开发了一个支持膜的漂浮的太阳的蒸气产生系统;后者是从空、多孔的 Ag/Au nanocubes 被装配的测微计大小的 colloidosome。由利用粒子联合的多尺度的 plasmonic,在 10 kWteries 的极其高太阳的热变换效率直到 92% ,做 heteroatom 的 nanofibers 展览高度可逆能力和令人满意的长期的骑车稳定性。osiers-sprout-like 做 heteroatom 的碳 nanofiber 电极交付骑车的 ultrastable 有 480 和 160 mAh 据的可逆能力的性能?? | Minmin Wang Jie Zhang Ping Wang Chuanping Li Xiaolong Xu Yongdong Jin | 2018 | Nano Research2018，11，7： | 4 |
| 14 | Designing Microarray and RNA-Seq Experiments for Greater Systems Biology Discovery in Modern Plant Genomics显示文摘Microarray 和 RNA-seq 实验成为了现代 genomics 和系统生物学的重要部分。从这些实验获得有意义的生物数据是要求靠近的注意到许多细节的一项艰巨任务。在任何步的疏忽能导致包含为模式抽取是不顺从的不适当或合成的信息的基因表示数据。因此,小心地在发射一个费时间、昂贵的实验前考虑试验性的设计是必要的。Contemporarily,大多数 genomics 实验有二个目的:(1 ) 产生二或为识别差别的更组可比较的数据在试验性的条件下面表示了基因,基因家庭,生物进程,或新陈代谢的小径;(2 ) 造本地基因规章的网络并且识别管理兴趣的生物过程和小径的层次地重要的管理者。因为第一个目的试图识别活跃分子的身份,第二为理解通过推断诱发性关系的内在的分子的机制由处理调停了提供一个基础,一个最佳的实验是生产生物学上相关、可引出的数据没有实质地增加费用,遇见两个目的。当开始 microarray 时,这评论讨论研究人员们通常面对的主要问题或 RNA-seq 试验并且总结试验性的设计的重要方面,它瞄准到研究人员们商讨怎么与低背景噪音,但是与更多的相互作用产生基因表情侧面在现代植物 genomics 便于新奇生物发现的帮助。 | Chuanping Yang Hairong Wei | 2015 | Molecular Plant2015，8，2： | 3 |
| 15 | Electrospun carbon nanofibers for lithium metal anodes:Progress and perspectives显示文摘Li metal anodes(LMAs)has attracted extensive research interest because of its extremely high theoretical capacity(3860 m Ah/g)at low redox potential(-3.04 V vs.standard hydrogen electrode).However,the extremely high chemical reactivity and the intrinsic“hostless”nature of LMAs bring about serious dendritic growth and dramatic volume change during the plating/strapping process,thus resulting in poor Coulombic efficiency,short lifespan,and severe safety concerns.Of various strategies,the construction of three-dimensional carbonaceous scaffolds for LMAs can substantially reduce the local current density,inhibit Li dendrite growth,and accommodate volume variation.Electrospinning is a simple yet effective strategy to fabricate carbon nanofibers(CNFs),which have been regarded as promising skeletons for LMAs,owing to their large surface areas,good electrical conductivity,and high porosity.In this Mini Review,we briefly introduce the fabrication of CNFs using electrospinning and the modification of CNFs.We highlight the recent advances in electrospun CNF skeletons for LMAs,including pure CNF and CNF-based composite scaffolds.Finally,we discuss the remaining challenges of electrospun CNF scaffolds for LMAs and provide possible solutions to push forward the advancement in this field. | Hongyang Chen Manxian Li Chuanping Li Xuan Li Yaling Wu Xiaochuan Chen Junxiong Wu Xiaoyan Li Yuming Chen | 2022 | Chinese Chemical Letters2022，33，1： | 3 |
| 16 | Control of mean and fluctuating forces on a circular cylinder at high Reynolds numbers显示文摘狭窄的脱衣被用来从 2.0 ×在雷纳兹数字在圆形的柱体上控制吝啬、波动的力量 10 4 到 1.0 × 10 5 。长带和柱体的斧子是平行的。控制参数是攻击和距离的角度从柱体描绘的长带宽度比率和长带位置。从柱体的双方流的旋涡能被压制的风隧道测试表演,和平均数拖,如果长带柱体下游地在一个有效地区被安装,柱体上的波动的电梯能被减少。单音方面的旋涡流的现象被发现。速度侧面在的导致长带的本地变化近柱体醒来被测量,并且在基础吸之间的关系和山峰价值尽最大努力,波动的电梯的光谱被学习。控制机理然后从不同观点被讨论。 | Chuanping Shao Jianming Wang | 2007 | Acta Mechanica Sinica2007，23，2： | 3 |
| 17 | Different effects of foliar application of silica sol on arsenic translocation in rice under low and high arsenite stress显示文摘Foliar application of Si can generally reduce As translocation from roots to shoots in rice;however, it does not always work, particularly under high As stress. Here, the effects of foliar application of nanoscale silica sol on As accumulation in rice were investigated under low(2 μmol/L) and high(8 μmol/L) arsenite stress. The results revealed that foliar Si application significantly decreased the As concentration in shoots under low arsenite stress, but showed different effects under high arsenite stress after 7 days of incubation. The reduction in root-to-shoot As translocation under the 2 As + Si treatment was related to the down-regulation of Os Lsi1 and Os Lsi2 expression and up-regulation of Os ABCC1 expression in roots. In the 8 As + Si treatment, the expressions of Os Lsi1, Os Lsi2, and Os ABCC1 were significantly promoted, which resulted in substantially higher As accumulation in both the roots and shoots. In the roots, As predominantly accumulated in the symplasts(90.6%–98.3%), in which the majority of As was sequestered in vacuoles(79.0%–94.0%) under both levels of arsenite stress. Compared with that of the 8 As treatment, the 8 As + Si treatment significantly increased the As concentration in cell walls, but showed no difference in the vacuolar As concentration, which remained constant at approximately 69.1–71.7 mg/kg during days 4–7. It appeared that the capacity of root cells to sequester As in the vacuoles had a threshold, and the excess As tended to accumulate in the cell walls and transfer to the shoots via apoplasts under high arsenite stress. This study provides a better understanding of the different effects of foliar Si application on As accumulation in rice from the view of arseniterelated gene expression and As subcellular distribution in roots. | Dandan Pan Chuanping Liu Jicai Yi Xiaomin Li Fangbai Li | 2021 | Journal of Environmental Sciences2021，33，7： | 3 |
| 18 | Screening of Transgenic Soybean Transformed by Means of Pollen-tube Using Kanamycin显示文摘Kanamycin was used to screen T0 seeds of the variety Dongnong 46 transformed by means of pollen-tube method. The results showed that 400 mg·L-1 kanamycin could inhibit growth of non-transgenic plants, and 2 positive plants were gotten combined with Gus dyeing and PCR detection. It is proved that this method is e-conomic and effective in preliminary screening the transgenic plants. | ZHANG Shuzhen XU Pengfei ZHANG Dayong LIN Shifeng LI Wenbin HAN Yingpeng YANG Chuanping | 2006 | Journal of Northeast Agricultural University(English Edition)2006，13，1： | 2 |
| 19 | mTOR regulates TLR-induced c-fos and Th1 responses to HBV and HCV vaccines显示文摘Although IL-12 plays a critical role in priming Th1 and cytotoxic T lymphocyte(CTL) responses, Toll-like receptor(TLR) signaling only induces low amounts of IL-12 in dendritic cells and macrophages, implying the existence of stringent regulatory mechanisms. In this study, we sought to uncover the mechanisms underlying TLR-induced IL-12 expression and the Th1 response. By systemic screening, we identified a number of protein kinases involved in the regulation of TLRinduced IL-12 expression. In particular, PI3 K, ERK, and m TOR play critical roles in the TLR-induced Th1 response by regulating IL-12 and IL-10 production in innate immune cells. Moreover, we identified c-fos as a key molecule that mediates m TOR-regulated IL-12 and IL-10 expression in TLR signaling. Mechanistically, m TOR plays a crucial role in c-fos expression, thereby modulating NFκB binding to promoters of IL-12 and IL-10. By controlling the expression of a special innate gene program, m TOR can specifically regulate the TLR-induced T cell response in vivo. Furthermore, blockade of m TOR by rapamycin efficiently boosted TLR-induced antigen-specific T and B cell responses to HBV and HCV vaccines. Taken together, these results reveal a novel mechanism through which m TOR regulates TLR-induced IL-12 and IL-10 production, contributing new insights for strategies to improve vaccine efficacy. | Li He Aiping Zang Min Du Dapeng Ma Chuanping Yuan Chun Zhou Jing Mu Huanjing Shi Dapeng Li Xulin Huang Qiang Deng Jianhua Xiao Huimin Yan Lijian Hui Ke Lan Sidong Xiong Xiaoxia Li Zhong Huang Hui Xiao | 2015 | Virologica Sinica2015，30，3： | 2 |
| 20 | Stimulation impact of electric currents on heterotrophic denitrifying microbial viability and denitrification performance in high concentration nitrate-contaminated wastewater显示文摘Electric current stimulation has been shown to have a positive influence on heterotrophic denitrifying microbial viability and has the potential to improve wastewater denitrification performance. This study investigated the effects of varying current densities on microbial activity and NO_3^- removal efficiency under heterotrophic conditions.NO_3^-removal rate was highest at an applied current density of 400 mA/m^2. However, the optimum removal efficiency of total inorganic nitrogen(TIN; 99%) was achieved when the current density was fixed at 200 m A/m^2. Accumulation of NH_4^+-N and NO_2^--N byproducts were also minimized at this current density. The activity of heterotrophic denitrifying microorganisms was much higher at both 200 and 400 mA/m^2. Moreover, the average adenosine-5′-triphosphate(ATP)content(an indicator of cell metabolism) at a current density of 1600 mA/m^2 was lower than that under no current, indicating heterotrophic denitrifying microbial activity can be inhibited at high current densities. Hence, direct electrical stimulation on the activity of heterotrophic denitrifying microorganisms in the developed system should be lower than 1600 mA/m^2. This study improves the understanding of electric current influence on heterotrophic denitrifying microorganisms and promotes the intelligent application of direct electrical stimulation on wastewater treatment processes. | Shuang Tong Hengyuan Liu Chuanping Feng Nan Chen Yan Zhao Baocai Xu Jiamin Zhao Ming Zhu | 2019 | Journal of Environmental Sciences2019，31，3： | 2 |
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