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| 1 | c-Fos overexpression increases the proliferation of human hepatocytes by stabilizing nuclear Cyclin D1显示文摘AIM: To investigate the effect of stable c-Fos overexpression on immortalized human hepatocyte (IHH) proliferation. METHODS: IHHs stably transfected with c-Fos (IHH-Fos) or an empty vector (IHH-C) were grown in medium supplemented with 1% serum or stimulated with 10% serum. Cell proliferation was assessed by cell counts, 3H-thymidine uptake and fl ow cytometry analyses. The levels of cell cycle regulatory proteins (Cyclin D1, E, A) cyclin dependent kinases (cdk) cdk2, cdk4, cdk6, and their inhibitors p15, p16, p21, p27, to- tal and phosphorylated GSK-3β and epidermal growth factor receptor (EGF-R) were assayed by Western blot- ting. Analysis of Cyclin D1 mRNA levels was performed by reverse transcription-polymerase chain reaction and real-time polymerase chain reaction (PCR) analysis. Stability of Cyclin D1 was studied by cycloheximide blockade experiments. RESULTS: Stable c-Fos overexpression increased cell proliferation under low serum conditions and resulted in a two-fold increase in [3H]-thymidine incorpora- tion following serum addition. Cell cycle analysis by fl ow cytometry showed that c-Fos accelerated the cell cycle kinetics. Following serum stimulation, Cyclin D1 was more abundantly expressed in c-Fos overexpressing cells. Cyclin D1 accumulation did not result from increased transcriptional activation, but from nuclear stabilization. Overexpression of c-Fos correlated with higher nuclear levels of inactive phosphorylated GSK- 3β, a kinase involved in Cyclin D1 degradation and higher levels of EGF-R mRNA, and EGF-R protein compared to IHH-C both in serum starved, and in serum stimulated cells. Abrogation of EGF-R signalling in IHH- Fos by treatment with AG1478, a specif ic EGF-R tyrosine kinase inhibitor, prevented the phosphorylation of GSK-3β induced by serum stimulation and decreased Cyclin D1 stability in the nucleus. CONCLUSION: Our results clearly indicate a positive role for c-Fos in cell cycle regulation in hepatocytes. Importantly, we delineate a new mechanism by which c-Fos could contribute to hepatocarcinogenesis through stabilization of Cyclin D1 within the nucleus, evoking a new feature to c-Fos implication in hepatocellular carcinoma. | Meryem Güller Kahina Toualbi-Abed Agnès Legrand Laurence Michel Alain Mauviel Dominique Bernuau Fanny Daniel | 2008 | World Journal of Gastroenterology2008，14，41： | 6 |
| 2 | Hepatic Resection for Noncolorectal Nonendocrine Liver Metastases: Analysis of 1452 Patients and Development of a Prognostic Model显示文摘 | René Adam Laurence Chiche Thomas Aloia Dominique Elias Rémy Salmon Michel Rivoire Daniel Jaeck Jean Saric Yves Patrice Le Treut Jacques Belghiti Georges Mantion Gilles Mentha | 2006 | Annals of Surgery2006，，4： | 2 |
| 3 | Biomarker discovery for inflammatory bowel disease, using proteomic serum profiling显示文摘 | Marie-Alice Meuwis Marianne Fillet Pierre Geurts Dominique de Seny Laurence Lutteri Jean-Paul Chapelle Vincent Bours Louis Wehenkel Jacques Belaiche Michel Malaise Edouard Louis Marie-Paule Merville | 2007 | Biochemical Pharmacology2007，，9： | 2 |
| 4 | Pharma- cology and clinical use of foscamet显示文摘 | Laurence Gerard Dominique Salmon-Ceronb | 1995 | International Jour- nal of Antimicrobial1995，5，4： | 1 |
| 5 | Hepatic Resection for Noncolorectal Nonendocrine Liver Metastases: Analysis of 1452 Patients and Development of a Prognostic Model显示文摘 | René Adam Laurence Chiche Thomas Aloia Dominique Elias Rémy Salmon Michel Rivoire Daniel Jaeck Jean Saric Yves Patrice Le Treut Jacques Belghiti Georges Mantion Gilles Mentha | 2006 | Annals of Surgery2006，，4： | 1 |
| 6 | A Compositional Model for the Functional Verification of High-level Synthesis Results显示文摘 | Dominique B Julia D Laurence P | 2000 | IEEE VLSI Systems2000，8，5： | 1 |
| 7 | Interleukin-18 and the Risk of Coronary Heart Disease in European Men: The Prospective Epidemiological Study of Myocardial Infarction (PRIME)显示文摘 | Stefan Blankenberg Gérald Luc Pierre Ducimetière Dominique Arveiler Jean Ferrières Philippe Amouyel Alun Evans Fran?ois Cambien Laurence Tiret | 2003 | Circulation: Journal of the American Heart Association2003，，20： | 1 |
| 8 | Heat induced formation of beta-lactoglobulin microgels driven by addition of calcium ions显示文摘 | Tuan Phan-Xuan Dominique Durand Taco Nicolai Laurence Donato Christophe Schmitt Lionel Bovetto | 2012 | Food Hydrocolloids2012，，： | 1 |
| 9 | Mesenchymal stromal cells use PGE2 to modulate activation and proliferation of lymphocyte subsets: Combined comparison of adipose tissue, Wharton’s Jelly and bone marrow sources显示文摘 | Mehdi Najar Gordana Raicevic Hicham Id Boufker Hussein Fayyad Kazan Cécile De Bruyn Nathalie Meuleman Dominique Bron Michel Toungouz Laurence Lagneaux | 2010 | Cellular Immunology2010，，2： | 1 |
| 10 | Longitudinal Study of Anti-Candida albicans Mucosal Immunity Against Aspartic Proteinases in HIV-Infected Patients显示文摘 | Laurence Millon Christine Drobacheff Renaud Piarroux Michel Monod Dominique Meillet Gabriel Reboux René Laurent | 2001 | JAIDS Journal of Acquired Immune Deficiency Syndromes2001，，2： | 1 |
| 11 | Combined Heterozygous Deficiency of the Classical Complement Pathway Proteins C2 and C4显示文摘 | Dominique Hartmann Veronique Fremeaux-Bacchi Laurence Weiss Alice Meyer Jacques Blouin Georges Hauptmann Michel Kazatchkine Beatrice Uring-Lambert | 1997 | Journal of Clinical Immunology1997，，： | 1 |
| 12 | A compositional model for the functional verification of high-level synthesis results 显示文摘 | Dominique B Julia D Laurence P | 2000 | IEEE VLSI Systems2000，8，5： | 1 |
| 13 | Accuracy and stability in incompressible SPH(ISPH)based on the projection method and a new approach显示文摘 | Xu Rui Stansby Peter Laurence Dominique | 2009 | Journal of Computational Physics2009，228，： | 1 |
| 14 | Interleukin-18 and the Risk of Coronary Heart Disease in European Men: The Prospective Epidemiological Study of Myocardial Infarction (PRIME)显示文摘 | Stefan Blankenberg Gérald Luc Pierre Ducimetière Dominique Arveiler Jean Ferrières Philippe Amouyel Alun Evans Fran?ois Cambien Laurence Tiret | 2003 | Circulation: Journal of the American Heart Association2003，，20： | 1 |
| 15 | Clinical Aspects and Prognosis of Brugada Syndrome in Children显示文摘 | Vincent Probst Isabelle Denjoy Paola G. Meregalli Jean-Christophe Amirault Fréderic Sacher Jacques Mansourati Dominique Babuty Elisabeth Villain Jacques Victor Jean-Jacques Schott Jean-Marc Lupoglazoff Philippe Mabo Christian Veltmann Laurence Jesel Phili | 2007 | Circulation2007，，15： | 1 |
| 16 | Lack of association between complement factor H polymorphisms and coronary artery disease or myocardial infarction显示文摘 | Viviane Nicaud Carole Francomme Jean-Bernard Ruidavets Gérald Luc Dominique Arveiler Frank Kee Alun Evans Caroline Morrison Stefan Blankenberg Fran?ois Cambien Laurence Tiret | 2007 | Journal of Molecular Medicine2007，，7： | 1 |
| 17 | Heavy particle dispersion from a point source in turbulent pipe flow显示文摘 | BERROUK A S STOCK D E DOMINIQUE LAURENCE | 2008 | International Journal of Multiphase Flow2008，34，： | 1 |
| 18 | Glycosaminoglycans inhibit the adherence and the spreading of osteoclasts and their precursors: Role in osteoclastogenesis and bone resorption显示文摘 | Marc Baud’huin Carmen Ruiz-Velasco Ga?tan Jego Céline Charrier Nijole Gasiunas John Gallagher Mike Maillasson Annamaria Naggi Marc Padrines Fran?oise Redini Laurence Duplomb Dominique Heymann | 2010 | European Journal of Cell Biology2010，，1： | 1 |
| 19 | Impact of different mesenchymal stromal cell types on T-cell activation, proliferation and migration显示文摘 | Mehdi Najar Gordana Raicevic Hussein Fayyad-Kazan Cécile De Bruyn Dominique Bron Michel Toungouz Laurence Lagneaux | 2013 | International Immunopharmacology2013，，4： | 1 |
| 20 | Carboplatin and weekly paclitaxel doublet chemotherapy compared with monotherapy in elderly patients with advanced non-small-cell lung cancer: IFCT-0501 randomised, phase 3 trial显示文摘 | Elisabeth Quoix Gérard Zalcman Jean-Philippe Oster Virginie Westeel Eric Pichon Armelle Lavolé Jér?me Dauba Didier Debieuvre Pierre-Jean Souquet Laurence Bigay-Game Eric Dansin Michel Poudenx Olivier Molinier Fabien Vaylet Denis Moro-Sibilot Dominique Her | 2011 | The Lancet2011，，9796： | 1 |
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