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| 1 | Individualized prediction of perineural invasion in colorectal cancer: development and validation of a radiomics prediction model显示文摘Objective: To develop and validate a radiomics prediction model for individualized prediction of perineural invasion(PNI) in colorectal cancer(CRC).Methods: After computed tomography(CT) radiomics features extraction, a radiomics signature was constructed in derivation cohort(346 CRC patients). A prediction model was developed to integrate the radiomics signature and clinical candidate predictors [age, sex, tumor location, and carcinoembryonic antigen(CEA) level]. Apparent prediction performance was assessed. After internal validation, independent temporal validation(separate from the cohort used to build the model) was then conducted in 217 CRC patients. The final model was converted to an easy-to-use nomogram.Results: The developed radiomics nomogram that integrated the radiomics signature and CEA level showed good calibration and discrimination performance [Harrell's concordance index(c-index): 0.817; 95% confidence interval(95% CI): 0.811–0.823]. Application of the nomogram in validation cohort gave a comparable calibration and discrimination(c-index: 0.803; 95% CI: 0.794–0.812).Conclusions: Integrating the radiomics signature and CEA level into a radiomics prediction model enables easy and effective risk assessment of PNI in CRC. This stratification of patients according to their PNI status may provide a basis for individualized auxiliary treatment. | Yanqi Huang Lan He Di Dong Caiyun Yang Cuishan Liang Xin Chen Zelan Ma Xiaomei Huang Su Yao Changhong Liang Jie Tian Zaiyi Liu | 2018 | Chinese Journal of Cancer Research2018，30，1： | 23 |
| 2 | Cell–cell contact with proinflammatory macrophages enhances the immunotherapeutic effect of mesenchymal stem cells in two abortion models显示文摘Mesenchymal stem cells(MSCs),which are pluripotent cells with immunomodulatory properties,have been considered good candidates for the therapy of several immune disorders,such as inflammatory bowel diseases,concanavalin A-induced liver injury,and graft-versus-host disease.The embryo is a natural allograft to the maternal immune system.A successful pregnancy depends on the timely extinction of the inflammatory response induced by embryo implantation,followed by the switch to a tolerant immune microenvironment in both the uterus and the system.Excessive infiltration of immune cells and serious inflammatory responses are triggers for embryo rejection,which results in miscarriage.Here,we demonstrated that adoptive transfer of MSCs could prevent fetal loss in a lipopolysaccharide(LPS)-induced abortion model and immune response-mediated spontaneous abortion model.The immunosuppressive MSCs alleviated excessive inflammation by inhibiting CD4+T cell proliferation and promoting the decidual macrophage switch to M2 in a tumor necrosis factor-stimulated gene-6(TSG-6)-dependent manner.Cell-tocell contact with proinflammatory macrophages increased the TSG-6 production by the MSCs,thereby enhancing the suppressive regulation of T cells and macrophages.Moreover,proinflammatory macrophages in contact with the MSCs upregulated the expression of CD200 on the stem cells and facilitated the reprogramming of macrophages towards an anti-inflammatory skew through the interaction of CD200 with CD200R on proinflammatory macrophages.Therefore,the results demonstrate that a TSG-6-mediated paracrine effect,reinforced by cell-to-cell contact between MSCs and proinflammatory macrophages,is involved in the mechanism of MSC-mediated abortion relief through the induction of immune tolerance.Our study also indicates the potential application of MSCs in clinical recurrent miscarriages. | Yanhong Li Di Zhang Ling Xu Lin Dong Ji Zheng Yikong Lin Jiefang Huang Yanyun Zhang Yu Tao Xingxing Zang Dajin Li Meirong Du | 2019 | Cellular & Molecular Immunology2019，16，12： | 23 |
| 3 | Future Changes and Uncertainties in Temperature and Precipitation over China Based on CMIP5 Models显示文摘Climate changes in future 21 st century China and their uncertainties are evaluated based on 22 climate models from the Coupled Model Intercomparison Project Phase 5(CMIP5). By 2081–2100, the annual mean surface air temperature(SAT) is predicted to increase by 1.3℃± 0.7℃, 2.6℃± 0.8℃ and 5.2℃± 1.2℃ under the Representative Concentration Pathway(RCP) scenarios RCP2.6, RCP4.5 and RCP8.5, relative to 1986–2005, respectively. The future change in SAT averaged over China increases the most in autumn/winter and the least in spring, while the uncertainty shows little seasonal variation.Spatially, the annual and seasonal mean SAT both show a homogeneous warming pattern across China, with a warming rate increasing from southeastern China to the Tibetan Plateau and northern China, invariant with time and emissions scenario.The associated uncertainty in SAT decreases from northern to southern China. Meanwhile, by 2081–2100, the annual mean precipitation increases by 5% ± 5%, 8% ± 6% and 12% ± 8% under RCP2.6, RCP4.5 and RCP8.5, respectively. The national average precipitation anomaly percentage, largest in spring and smallest in winter, and its uncertainty, largest in winter and smallest in autumn, show visible seasonal variations. Although at a low confidence level, a homogeneous wetting pattern is projected across China on the annual mean scale, with a larger increasing percentage in northern China and a weak drying in southern China in the early 21 st century. The associated uncertainty is also generally larger in northern China and smaller in southwestern China. In addition, both SAT and precipitation usually show larger seasonal variability on the sub-regional scale compared with the national average. | TIAN Di GUO Yan DONG Wenjie | 2015 | Advances in Atmospheric Sciences2015，32，4： | 21 |
| 4 | Experimental research on electrochemical machining of titanium alloy Ti60 for a blisk显示文摘Ti60(Ti–5.6Al–4.8Sn–2Zr–1Mo–0.35Si–0.7Nd) is a high-temperature titanium alloy that is now used for important components of aircraft engines. Electrochemical machining(ECM) is a promising technique that has several advantages, such as a high machining rate, and can be used on a wide range of difficult-to-process materials. In this paper, orthogonal experiments are conducted to investigate ECM of Ti60, with the aim of determining the influences of some electrochemical process parameters on the surface roughness. The most important parameter is found to be the frequency of the pulsed power supply. It is found that using suitably optimized parameters for ECM can greatly decrease the surface roughness of a workpiece. A surface roughness of approximately 0.912 lm can be obtained with the following optimal parameters: Na Cl electrolyte concentration 13wt%, voltage20 V, pulse frequency 0.4 k Hz, duty cycle 0.3, temperature 23 °C, and anode feed rate 0.5 mm/min.Furthermore, blisk blades have been successfully processed using these optimized parameters. | Chen Xuezhen Xu Zhengyang Zhu Dong Fang Zhongdong Zhu Di | 2016 | Chinese Journal of Aeronautics2016，29，1： | 17 |
| 5 | Biological properties of neural progenitor cells isolated from the hippocampus of adult cynomolgus monkeys显示文摘Background The existence of neurogenesis in the hippocampus of adult nonhuman primates has been confirmed in recent years, however, the biological properties of adult neural stem cells or neural progenitor cells (NPCs) from this region remain to be extensively explored. The present work was to investigate on the expansion of NSCs/NPCs from the hippocampus of adult cynomolgus monkeys and the examination of their characteristics in vitro. Methods NPCs isolated from the hippocampus of adult cynomolgus monkeys were expanded in vitro in serum-free media containing growth factors, and were then allowed to differentiate by removing mitotic factors. The expansion capacity of NPCs and their differentiation potential were assayed by immunohistochemical and immunocytochemical analysis. Results During primary culture, NPCs underwent cell division, proliferation and aggregation to form neurospheres that were growing in suspension. Without mitotic stimulation, most neurospheres adhered to the culture dish and started to differentiate. Eventually, nearly 12% of the differentiated cells expressed neuron specific marker-βIII-tubulin (Tuj1) and 84% expressed astrocyte specific marker-fibrillary acidic protein (GFAP). In addition, the expression of a neural stem cell marker, nestin, was found both in NPCs and in the subgranular zone of adult monkey hippocampus, where NPCs were originally derived. Conclusions NPCs from the hippocampus of adult cynomolgus monkeys can be expanded to some extent in vitro and are capable of differentiating into neurons and astrocytes. Further experiments to promote the in vitro proliferation capacity of NPCs will be required before adult NPCs can be used as a useful cell model for studying adult neurogenesis and cell replacement therapy using adult stem cells. | YUE Feng CHEN Biao WU Di DONG Kai ZENG Si-en ZHANG Yu | 2006 | Chinese Medical Journal2006，，2： | 16 |
| 6 | Trajectory control strategy of cathodes in blisk electrochemical machining显示文摘A turbine blisk, which combines blades and a disk together, is one of the most important components of an aero engine. In the process of blisk electrochemical machining (ECM), the sheet cathode, which is usually used as a tool electrode, has a complicated structure. In addition to that, the channel between the adjacent blades is narrow and twisted, so interference is apt to happen when the sheet cathode feeds into the channel. Therefore, it is important to choose suitable trajectory control strategy. In this paper, a new trajectory control strategy of the sheet cathode is presented and corresponding simulation analysis is conducted on the basis of an actual blisk model. The simulation results demonstrate that the sheet cathode can feed into the channel by a spatial line trajectory without interference. Moreover, the verification experiments are carried out according to the simulation. The experimental results show that the cathode can move into the channel without interference. It is verified that the new trajectory control strategy is correct and can be used in the blisk ECM process successfully. | Zhu Dong Zhu Di Xu Zhengyang Zhou Laishui | 2013 | Chinese Journal of Aeronautics2013，26，4： | 14 |
| 7 | Modeling of radionickel sorption on MX-80 bentonite as a function of pH and ionic strength显示文摘MX-80 bentonite was detected using acid-based titration, XRD and FTIR in detail. The sorption behavior of 63Ni(Ⅱ) from aqueous solution to MX-80 bentonite was investigated as a function of solid content, ionic strength and pH by using batch technique. The experimental data of 63Ni(Ⅱ) sorption on MX-80 bentonite was obtained using the diffuse layer model (DLM) with the aid of FITEQL 3.1 program. The results indicated that the sorption of 63Ni(Ⅱ) on MX-80 bentonite was mainly dominated by surface complexation, and cation exchange also contributed partly to 63Ni(Ⅱ) sorption at low pH values. The sorption isotherms were simulated by Langmuir and Freundlich models, and the results indicated that Freundlich isotherm model fitted the sorption data better than the Langmuir isotherm model. The results are crucial to evaluate the sorption and migration of radionickel in MX-80 bentonite. | SHAO DaDong XU Di WANG SuoWei FANG QiaoHui WU WangSuo DONG YunHui WANG XiangKe | 2009 | Science China Chemistry2009，52，3： | 13 |
| 8 | Supra-(carbon nanodots) with a strong visible to near-infrared absorption band and efficient photothermal conversion显示文摘A novel concept and approach to engineering carbon nanodots(CNDs)were explored to overcome the limited light absorption of CNDs in low-energy spectral regions.In this work,we constructed a novel type of supra-CND by the assembly of surface charge-confined CNDs through possible electrostatic interactions and hydrogen bonding.The resulting supra-CNDs are the first to feature a strong,well-defined absorption band in the visible to near-infrared(NIR)range and to exhibit effective NIR photothermal conversion performance with high photothermal conversion efficiency in excess of 50%. | Di Li Dong Han Song-Nan Qu Lei Liu Peng-Tao Jing Ding Zhou Wen-Yu Ji Xiao-Yun Wang Tong-Fei Zhang De-Zhen Shen | 2016 | Light(Science & Applications)2016，5，1： | 12 |
| 9 | Mechanisms of improvement of left ventricle remodeling by transplanting two kinds of autologous bone marrow stem cells in pigs显示文摘背景在尖锐心肌的梗塞( AMI )以后的很多心肌的房间的坏死导致心脏的功能和移植能改进的室 remodeling.Stem 房间的减少在 AMI 以后的心脏的功能,但是包含的机制一直不是完全学习试图在改变的室上经由冠的动脉调查自体同源的骨头髓 mononuclear 房间( BM-MNC )和 rnesenchymal 干细胞( MSC )的移植的效果的在场的 understood.The 控制组,简单梗塞模型组, BM-MNC 移植组,和 MSC 移植当有 AMI 的一个缩小的猪的模型被建立时, 90 纪录的 group.At ,自体同源的 BM-MNC 的移植(( 4.7ntraoperative ,并且手术后)被评估, compared.The 病态和死亡结果在二 groups.The 后续结果被比较,在二组织的 surgery.Results 与外科手术前的高风险的侧面有病人的类似的百分比以后。 | LI Shu-ren QI Xiao-yong HU Fu-li ZHANG Jian-qing WANG Tian-hong DANG Yi MENG Cun-liang LIU Hui-liang LI Ying-xiao WU Di DONG Jie XUN Li-ying GAO Li-hui JIN Fu-chang | 2008 | Chinese Medical Journal2008，，23： | 12 |
| 10 | The enhanced X-ray Timing and Polarimetry mission—eXTP显示文摘In this paper we present the enhanced X-ray Timing and Polarimetry mission—eXTP. eXTP is a space science mission designed to study fundamental physics under extreme conditions of density, gravity and magnetism. The mission aims at determining the equation of state of matter at supra-nuclear density, measuring effects of QED, and understanding the dynamics of matter in strong-field gravity. In addition to investigating fundamental physics, eXTP will be a very powerful observatory for astrophysics that will provide observations of unprecedented quality on a variety of galactic and extragalactic objects. In particular, its wide field monitoring capabilities will be highly instrumental to detect the electro-magnetic counterparts of gravitational wave sources.The paper provides a detailed description of:(1) the technological and technical aspects, and the expected performance of the instruments of the scientific payload;(2) the elements and functions of the mission, from the spacecraft to the ground segment. | ShuangNan Zhang Andrea Santangelo Marco Feroci YuPeng Xu FangJun Lu Yong Chen Hua Feng Shu Zhang Sφren Brandt Margarita Hernanz Luca Baldini Enrico Bozzo Riccardo Campana Alessandra De Rosa YongWei Dong Yuri Evangelista Vladimir Karas Norbert Meidinger Aline Meuris Kirpal Nandra Teng Pan Giovanni Pareschi Piotr Orleanski QiuShi Huang Stephane Schanne Giorgia Sironi Daniele Spiga Jiri Svoboda Gianpiero Tagliaferri Christoph Tenzer Andrea Vacchi Silvia Zane Dave Walton ZhanShan Wang Berend Winter Xin Wu Jean J.M.in't Zand Mahdi Ahangarianabhari Giovanni Ambrosi Filippo Ambrosino Marco Barbera Stefano Basso Jörg Bayer Ronaldo Bellazzini Pierluigi Bellutti Bruna Bertucci Giuseppe Bertuccio Giacomo Borghi XueLei Cao Franck Cadoux Francesco Ceraudo TianXiang Chen Yu Peng Chen Jerome Chevenez Marta Civitani Wei Cui WeiWei Cui Thomas Dauser Ettore Del Monte Sergio Di Cosimo Sebastian Diebold Victor Doroshenko Michal Dovciak YuanYuan Du Lorenzo Ducci QingMei Fan Yannick Favre Fabio Fuschino JoséLuis Ga'lvez Min Gao MingYu Ge Olivier Gevin Marco Grassi QuanYing Gu YuDong Gu DaWei Han Bin Hong Wei Hu Long Ji ShuMei Jia WeiChun Jiang Thomas Kennedy Ingo Kreykenbohm Irfan Kuvvetli Claudio Labanti Luca Latronico Gang Li MaoShun Li Xian Li Wei Li ZhengWei Li Olivier Limousin HongWei Liu XiaoJing Liu Bo Lu Tao Luo Daniele Macera Piero Malcovati Adrian Martindale Malgorzata Michalska Bin Meng Massimo Minuti Alfredo Morbidini Fabio Muleri Stephane Paltani Emanuele Perinati Antonino Picciotto Claudio Piemonte JinLu Qu Alexandre Rachevski Irina Rashevskaya Jerome Rodriguez Thomas Schanz ZhengXiang Shen LiZhi Sheng JiangBo Song LiMing Song Carmelo Sgro Liang Sun Ying Tan Phil Uttley Bo Wang DianLong Wang GuoFeng Wang Juan Wang LangPing Wang YuSa Wang Anna L.Watts XiangYang Wen Jörn Wilms ShaoLin Xiong JiaWei Yang Sheng Yang YanJi Yang Nian Yu WenDa Zhang Gianluigi Zampa Nicola Zampa Andrzej A.Zdziarski AiMei Zhang ChengMo Zhang Fan Zhang Long Zhang Tong Zhang Yi Zhang XiaoLi Zhang ZiLiang Zhang BaoSheng Zhao ShiJie Zheng Yu Peng Zhou Nicola Zorzi J.Frans Zwart | 2019 | Science China(Physics,Mechanics & Astronomy)2019，62，2： | 11 |
| 11 | A CFD/CSD model for aeroelastic calculations of large-scale wind turbines显示文摘In this paper,a CFD/CSD model coupling N-S equations and structural equations of motion in the time domain is described for aeroelastic analysis of large wind turbines.The structural modes of blades are analyzed with one-dimensional beam models.By combining point matched sliding grid for wind turbine rotation and deforming grid for structural vibrations,a hybrid dynamic grid strategy is designed for the multi-block structured grid system of a wind turbine.The dual time-stepping approach and finite volume scheme are applied to the three-dimensional unsteady preconditioned N-S equations,and DES approach is employed to simulate the unsteady massively separated flows.A modal approach is adopted to calculate the structural response,and a predictor-corrector scheme is used to solve the structural equations of motion.CFD and CSD solvers are tightly coupled via successive iterations within each physical time step.As a result,a time-domain CFD/CSD model for aeroelastic analysis of a large wind turbine is achieved.The presented method is applied to the NH1500 large wind turbine under the rated condition,and the calculated aeroelastic characteristics agree well with those of the prescribed vortex wake method. | GUO TongQing LU ZhiLiang TANG Di WANG TongGuang DONG Lu | 2013 | Science China(Technological Sciences)2013，56，1： | 11 |
| 12 | Science Letters:IGFBP7 plays a potential tumor suppressor role against colorectal carcinogenesis with its expression associated with DNA hypomethylation of exon 1显示文摘Insulin-like growth factor binding-protein-7 (IGFBP7) was obtained from our previous colonic adenocarcinoma (CRC) and normal mucosa suppression subtraction hybridization (SSH) cDNA libraries. By RT-PCR and immunohistochemistry, we found that IGFBP7 was overexpressed in CRC tissue compared to normal tissue. However, our in vitro experiments performed in 10 CRC cell lines showed that IGFBP7 expressed only in SW480 and Caco2 cell lines, which implied an underlying reversible regulatory mechanism. Using methylation-specific PCR (MSP) and bisulfite sodium PCR (BSP), we found that its expression was associated with DNA hypomethylation of exon1. This was further supported by the in vitro study which showed restored IGFBP7 expression after demethylation agent 5-aza-2’-deoxycytidine treatment. Correlation analysis between IGFBP7 expression and prognosis indicated that overexpression of IGFBP7 in CRC tissue correlated with favourable survival. Investigation of the func-tional role of IGFBP7 through transfection studies showed that IGFBP7 protein could inhibit growth rate, decrease colony for-mation activity, and induce apoptosis in RKO and SW620 cells, suggesting it a potential tumor suppressor protein in colorectal carcinogenesis. In conclusion, our study clearly demonstrated that IGFBP7 plays a potential tumor suppressor role against colo-rectal carcinogenesis and its expression is associated with DNA hypomethylation of exon 1. | RUAN Wen-jing LIN Jie XU En-ping XU Fang-ying MA Yu DENG Hong HUANG Qiong LV Bing-jian HU Hu CUI Jing DI Mei-juan DONG Jian-kang LAI Mao-de | 2006 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2006，7，11： | 11 |
| 13 | Future changes in coverage of 1.5℃ and 2℃warming thresholds显示文摘The areas covered by 1.5 ℃ and 2.0 ℃ warming thresholds under RCP2.6, RCP4.5, and RCP8.5 were analyzed based on 22 CMIP5 models. More than 90% of the model runs are in agreement that by the end of the 21 st century, near-surface air temperature changes over ~5%(~2%), ~40%(~18%), and ~92%(~86%)of the globe will cross the 1.5 ℃(2.0 ℃) threshold under RCP2.6, RCP4.5, and RCP8.5, respectively. Under RCP8.5, nearly the whole of North America, Europe + Russia, Africa, and Asia–Russia will cross the 1.5 ℃(2.0 ℃) threshold in ~2050(~2060), while the coverage rates over South America and Oceania are ~80%(~75%) and ~50%(~30%), respectively. The threshold-onset time(TOT) for 2 ℃ warming is earliest over Europe + Russia and North America, followed by Africa, Asia–Russia, South America, and finally Oceania under the RCP4.5 and RCP8.5 scenarios. The TOT for 1.5 ℃ is ~10–30 years ahead of that for 2.0 ℃. | di tian wenjie dong han zhang yan guo shili yang tanlong dai | 2017 | Science Bulletin2017，62，21： | 10 |
| 14 | Sequence stratigraphy, paleogeography, and coal accumulation regularity of major coal-accumulating periods in China显示文摘There are 9 major coal-accumulating periods during geological history in China,including the Early Carboniferous,Late Carboniferous-Early Permian,Middle Permian,Late Permian,Late Triassic,Early-Middle Jurassic,Early Cretaceous,Paleogene and Neogene.The coal formed in these periods were developed in different coal-accumulating areas(CAA)including the North China,South China,Northwest China,Northeast China,the Qinghai–Tibet area,and China offshore area.In this paper,we investigated depositional environments,sequence stratigraphy,lithofacies paleogeography and coal accumulation pattern of five major coal-accumulating periods including the Late Carboniferous to Middle Permian of the North China CAA,the Late Permian of the South China CAA,the Late Triassic of the South China CAA,the Early-Middle Jurassic of the North and Northwest China CAA,and the Early Cretaceous in the Northeast China CAA.According to distribution of the coal-bearing strata and the regional tectonic outlines,we have identified distribution range of the coal-forming basins,sedimentary facies types and coal-accumulating models.The sequence stratigraphic frameworks of the major coal-accumulating periods were established based on recognition of a variety of sequence boundaries.The distribution of thick coals and migration patterns of the coal-accumulating centers in the sequence stratigraphic framework were analyzed.The lithofacies paleogeography maps based on third-order sequences were reconstructed and the distribution of coal accumulation centers and coal-rich belts were predicted. | Longyi Shao Xuetian Wang Dongdong Wang Mingpei Li Shuai Wang Yingjiao Li Kai Shao Chao Zhang Caixia Gao Daxiao Dong Aiguo Cheng Jing Lu Congwei Ji Di Gao | 2020 | International Journal of Coal Science & Technology2020，7，2： | 9 |
| 15 | Impact of an innovative tuberculosis financing and payment model on health service utilization by tuberculosis patients in China:do the poor fare better than the rich?显示文摘Background:Tuberculosis(TB)prevalence is closely associated with poverty in China,and poor patients face more barriers to treatment.Using an insurance-based approach,the China-Gates TB program Phase II was implemented between 2012 and 2014 in three cities in China to improve access to TB care and reduce the financial burden on patients,particularly among the poor.This study aims to assess the program effects on service use,and its equity impact across different income groups.Methods:Data from 788 and 775 patients at baseline and final evaluation were available for analysis respectively.Inpatient and outpatient service utilization,treatment adherence,and patient satisfaction were assessed before and after the program,across different income groups(extreme poverty,moderate poverty and non-poverty),and in various program cities,using descriptive statistics and multi-variate regression models.Key stakeholder interviews were conducted to qualitatively evaluate program implementation and impacts.Results:After program implementation,the hospital admission rate increased more for the extreme poverty group(48.5 to 70.7%)and moderate poverty group(45.0 to 68.1%),compared to the non-poverty group(52.9 to 643%).The largest increase in the number of outpatient visits was also for the extreme poverty group(4.6 to 5.7).The proportion of patients with good medication adherence increased by 15 percentage points in the extreme poverty group and by ten percentage points in the other groups.Satisfaction rates were high in all groups.Qualitative feedback from stakeholders also suggested that increased reimbursement rates,easier reimbursement procedures,and allowance improved patients'service utilization.Implementation of case-based payment made service provision more compliant to clinical pathways.Conclusion:Patients in extreme or moderate poverty benefited more from the program compared to a non-poverty group,indicating improved equity in TB service access.The pro-poor design of the program provides important丨essons to other TB programs in China and other countries to better address TB care for the poor. | Di Dong Wei-Xi Jiang Qian Long Fei Huang Hui Zhang Jia-Ying Chen Li Xiang Qiang Li Sheng-Lan Tang Henry Lucas | 2019 | Infectious Diseases of Poverty2019，8，3： | 8 |
| 16 | Receptome profiling identifies KREMEN1 and ASGR1 as alternative functional receptors of SARS-CoV-2显示文摘Host cellular receptors play key roles in the determination of virus tropism and pathogenesis.However,little is known about SARS-CoV-2 host receptors with the exception of ACE2.Furthermore,ACE2 alone cannot explain the multi-organ tropism of SARS-CoV-2 nor the clinical differences between SARS-CoV-2 and SARS-CoV,suggesting the involvement of other receptor(s).Here,we performed genomic receptor profiling to screen 5054 human membrane proteins individually for interaction with the SARS-CoV-2 capsid spike(S)protein.Twelve proteins,including ACE2,ASGR1,and KREMEN1,were identified with diverse S-binding affinities and patterns.ASGR1 or KREMEN1 is sufficient for the entry of SARS-CoV-2 but not SARS-CoV in vitro and in vivo.SARS-CoV-2 utilizes distinct ACE2/ASGR1/KREMEN1(ASK)receptor combinations to enter different cell types,and the expression of ASK together displays a markedly stronger correlation with virus susceptibility than that of any individual receptor at both the cell and tissue levels.The cocktail of ASK-related neutralizing antibodies provides the most substantial blockage of SARS-CoV-2 infection in human lung organoids when compared to individual antibodies.Our study revealed an interacting host receptome of SARS-CoV-2,and identified ASGR1 and KREMEN1 as alternative functional receptors that play essential roles in ACE2-independent virus entry,providing insight into SARS-CoV-2 tropism and pathogenesis,as well as a community resource and potential therapeutic strategies for further COVID-19 investigations. | Yunqing Gu Jun Cao Xinyu Zhang Hai Gao Yuyan Wang Jia Wang Juan He Xiaoyi Jiang Jinlan Zhang Guanghui Shen Jie Yang Xichen Zheng Gaowei Hu Yuanfei Zhu Shujuan Du Yunkai Zhu Rong Zhang Jianqing Xu Fei Lan Di Qu Guoliang Xu Yun Zhao Dong Gao Youhua Xie Min Luo Zhigang Lu | 2022 | Cell Research2022，32，1： | 8 |
| 17 | Repeatability and Reproducibility of Quantitative Assessment of the Retinal Microvasculature Using Optical Coherence Tomography Angiography Based on Optical Microangiography显示文摘Objective The aim of this study was to determine the repeatability and reproducibility of optical coherence tomography angiography(OCTA) based on optical microangiography(OMAG) measurements of macular vessels in normal eyes. Methods In this prospective cohort study, 40 eyes of 40 healthy volunteers underwent repeated OCTA(Cirrus HD-OCT 5000 angiography system, Carl Zeiss Meditec, Inc.) scans on two separate visit days. On each visit day, the eyes were scanned three times. The following parameters were used to quantitatively describe the OCTA images of the superficial vascular network: vessel area density(VAD), vessel skeleton density(VSD), vessel diameter index(VDI), vessel perimeter index(VPI), vessel complexity index(VCI), flux, and foveal avascular zone(FAZ). Coefficient of variation(CV) and intraclass correlation coefficient(ICC) were calculated for evaluating intravisit and intervisit repeatability, as well as interobserver reproducibility. Results The measurements showed high repeatability [CVs ≤ 4.2%(intravisit) and ≤ 4.6%(intervisit)] and interobserver reproducibility(ICCs ≥ 0.923) for all parameters. Conclusion This study demonstrated good repeatability and reproducibility of OCTA based on OMAG for the measurement of superficial vessel parameters in normal eyes. | ZHAO Qi YANG Wen Li WANG Xiao Na Ruikang K Wang YOU Qi Sheng CHU Zhong Di XIN Chen ZHANG Meng Yu LI Dong Jun WANG Zi Yang CHEN Wei LI Yi Feng CUI Rui SHEN Lin WEI Wen Bin | 2018 | Biomedical and Environmental Sciences2018，31，6： | 8 |
| 18 | Climate change and its effect on reference crop evapotranspiration in central and western Inner Mongolia during 1961-2009显示文摘水资源是到在中央、西方的内部蒙古的农业生产的主要限制之一,在的地方由干旱、半干旱的气候是特征的。参考庄稼土壤水分蒸发蒸腾损失总量(et 0 ) 是在农业生态系统的水周期的重要部分,它在庄稼生长和产量上有直接效果。et 0 上的气候变化的含意具有为关于安排的水管理和灌溉的农业的高重要性。这研究的目的是在时期上在在中央、西方的内部蒙古的 et 0 上在气候和它的效果分析变化 1961 ~ 2009。为这个目的,在越过学习区域的十个气象学的车站的数据被收集, FAO Penman-Monteith 56 方法被使用。结果证明平均温度,最大的温度和最小的温度由 0.49 莬增加了吗?? | Di HE Yaling LIU Zhihua PAN Pingli AN Liwei WANG Zhiqiang DONG Jingting ZHANG Xuebiao PAN Peiyi ZHAO | 2013 | Frontiers of Earth Science2013，7，4： | 8 |
| 19 | Focal adhesion protein Kindlin-2 regulates bone homeostasis in mice显示文摘Our recent studies demonstrate that the focal adhesion protein Kindlin-2 is critical for chondrogenesis and early skeletal development. Here, we show that deleting Kindlin-2 from osteoblasts using the 2.3-kb mouse Col1 a1-Cre transgene minimally impacts bone mass in mice, but deleting Kindlin-2 using the 10-kb mouse Dmp1-Cre transgene, which targets osteocytes and mature osteoblasts, results in striking osteopenia in mice. Kindlin-2 loss reduces the osteoblastic population but increases the osteoclastic and adipocytic populations in the bone microenvironment. Kindlin-2 loss upregulates sclerostin in osteocytes,downregulates β-catenin in osteoblasts, and inhibits osteoblast formation and differentiation in vitro and in vivo. Upregulation ofβ-catenin in the mutant cells reverses the osteopenia induced by Kindlin-2 deficiency. Kindlin-2 loss additionally increases the expression of RANKL in osteocytes and increases osteoclast formation and bone resorption. Kindlin-2 deletion in osteocytes promotes osteoclast formation in osteocyte/bone marrow monocyte cocultures, which is significantly blocked by an anti-RANKLneutralizing antibody. Finally, Kindlin-2 loss increases osteocyte apoptosis and impairs osteocyte spreading and dendrite formation.Thus, we demonstrate an important role of Kindlin-2 in the regulation of bone homeostasis and provide a potential target for the treatment of metabolic bone diseases. | Huiling Cao Qinnan Yan Dong Wang Yumei Lai Bo Zhou Qi Zhang Wenfei Jin Simin Lin Yiming Lei Liting Ma Yuxi Guo Yishu Wang Yilin Wang Xiaochun Bai Chuanju Liu Jian QFeng Chuanyue Wu Di Chen Xu Cao Guozhi Xiao | 2020 | Bone Research2020，8，1： | 8 |
| 20 | Immunomodulatory activity of andrographolide on macrophage activation and specific antibody response显示文摘 | Wei WANG Jing WANG Sheng-fu DONG Chun-hong LIU Paola ITALIANI Shu-hui SUN Jing XU Diana BORAS-CHI Shi-ping MA Di QU | 2010 | Acta Pharmacologica Sinica2010，31，2： | 8 |
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