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207篇 您的检索式:作者名="Da Shen"
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1A two-step lineage reprogramming strategy to generate functionally competent human hepatocytes from fibroblasts显示文摘Terminally differentiated cells can be generated by lineage reprogramming,which is,however,hindered by incomplete conversion with residual initial cell identity and partial functionality. Here, we demonstrate a new reprogramming strategy by mimicking the natural regeneration route, which permits generating expandable hepatic progenitor cells and functionally competent human hepatocytes. Fibroblasts were first induced into human hepatic progenitor-like cells (hHPLCs), which could robustly expand in vitro and efficiently engraft in vivo. Moreover, hHPLCs could be efficiently induced into mature human hepatocytes (hiHeps) in vitro, whose molecular identity highly resembles primary human hepatocytes (PHHs). Most importantly, hiHeps could be generated in large quantity and were functionally competent to replace PHHs for drug-metabolism estimation, toxicity prediction and hepatitis B virus infection modeling. Our results highlight the advantages of the progenitor stage for successful lineage reprogramming. This strategy is promising for generating other mature human cell types by lineage reprogramming.Bingqing Xie Da Sun Yuanyuan Du Jun Jia Shicheng Sun Jun Xu Yifang Liu Chengang Xiang Sitong Chen Huangfan Xie Qiming Wang Guangya Li Xuehui LYU Hui Shen Shiyu Li Min Wu Xiaonan Zhang Yue Pu Kuanhui Xiang Weifeng Lai Peng Du Zhenghong Yuan Cheng Li Yan Shi Shichun Lu Hongkui Deng 2019Cell Research2019,29,9:9
2Effects of yaw-roll coupling ratio on the lateral-directional departure prediction and restraint显示文摘The experimental data obtained from yaw-roll coupled wind tunnel tests are used for lateral-directional departure prediction,by linearizing the_b model to extract nominal dynamic derivatives at each coupling ratio.The prediction results are compared with those of the existing engineering methods which are based on the conventional aerodynamic derivatives.The comparison shows that the yaw-roll coupling ratio has a great influence on the departure susceptibility.The departure resistance will loss in partial region of the coupling ratio when the angle of attack is higher than a critical value.According to the stable and unstable regions of coupling ratio,a two-segment stability augmentation system with two different feedback gain matrices is obtained by pole-placement method.The two-segment stability augmentation system is used in the simulations of straight and level flight,steady turn,spin recovery and Herbst maneuver.The simulation results are also compared with the applications of a fixed-gain stability augmentation system designed by the conventional aerodynamic derivatives.When the yaw-roll coupling effects are fully considered,the two-segment stability augmentation system is more effective for departure restraint and can provide a better flying quality with less control energy.Lin SHEN Da HUANG Genxing WU 2019Chinese Journal of Aeronautics2019,32,10:8
3Effects of yaw-roll coupling ratio on lateraldirectional aerodynamic characteristics显示文摘Experimental investigation of large amplitude yaw-roll coupled oscillations was conducted in a low-speed wind tunnel using an aircraft configuration model. A special test rig was designed and constructed to provide different coupled motions from low to high angles of attack.A parameter ‘‘coupling ratio' was introduced to indicate the extent of yaw-roll coupling. At each pitch angle, seven coupling ratios were designed to study the yaw-roll coupling effects on the lateraldirectional aerodynamic characteristics systematically. At high angles of attack, the damping characteristics of yawing and rolling moments drastically varied with coupling ratios. In the coupled motions with the rotation taking place about the wind axis, the lateral-directional aerodynamic moments exhibited unsteady characteristics and were different from the ‘‘quasi-steady' results of the rotary balance tests. The calculated results of the traditional aerodynamic derivative method were also compared with the experimental data. At low and very high angles of attack, the aerodynamic derivative method was applicative. However, within a wide range of angles of attack, the calculated results of aerodynamic derivative method were inconsistent with the experimental data, due to the drastic changes of damping characteristics of lateral-directional aerodynamic moments with yaw-roll coupling ratios.Lin SHEN Da HUANG Genxing WU 2019Chinese Journal of Aeronautics2019,32,2:7
4Characterization of atomic defects on the photoluminescence in two-dimensional materials using transmission electron microscope显示文摘Two-dimensional material(2D)that possesses atomic thin geometry and remarkable properties is a star material for the fundamental researches and advanced applications.Defects in 2D materials are critical and fundamental to understand the chemical,physical,and optical properties.Photoluminescence arises in 2D materials owing to various physical phenomena including activator/dopant-induced luminescence and defect-related emissions,and so forth.With the advanced transmission electron microscopy(TEM)technologies,such as aberration correction and low voltage technologies,the morphology,chemical compositions and electronic structures of defects in 2D material could be directly characterized at the atomic scale.In this review,we introduce the applications of state-of-the-art TEM technologies on the studies of the role of atomic defects in the photoluminescence characteristics in 2D material.The challenges in spatial and time resolution are also discussed.It is proved that TEM is a powerful tool to pinpoint the relationship between the defects and the photoluminescence characteristics.Jiayan Zhang Ye Yu Peng Wang Chen Luo Xing Wu Ziqi Sun Jianlu Wang Wei Da Hu Guozhen Shen 2019InfoMat2019,1,1:6
5DeST 3.0:A new-generation building performance simulation platform显示文摘Buildings contribute to almost 30%of total energy consumption worldwide.Developing building energy modeling programs is of great significance for lifecycle building performance assessment and optimization.Advances in novel building technologies,the requirements of high-performance computation,and the demands for multi-objective models have brought new challenges for building energy modeling software and platforms.To meet the increasing simulation demands,DeST 3.0,a new-generation building performance simulation platform,was developed and released.The structure of DeST 3.0 incorporates four simulation engines,including building analysis and simulation(BAS)engine,HVAC system engine,combined plant simulation(CPS)engine,and energy system(ES)engine,connected by air loop and water loop balancing iterations.DeST 3.0 offers numerous new simulation features,such as advanced simulation modules for building envelopes,occupant behavior and energy systems,cross-platform and compatible simulation kernel,FMI/FMU-based co-simulation functionalities,and high-performance parallel simulation architecture.DeST 3.0 has been thoroughly evaluated and validated using code verification,inter-program comparison,and case-study calibration.DeST 3.0 has been applied in various aspects throughout the building lifecycle,supporting building design,operation,retrofit analysis,code appliance,technology adaptability evaluation as well as research and education.The new generation building simulation platform DeST 3.0 provides an efficient tool and comprehensive simulation platform for lifecycle building performance analysis and optimization.Da Yan Xin Zhou Jingjing An Xuyuan Kang Fan Bu Youming Chen Yiqun Pan Yan Gao Qunli Zhang Hui Zhou Kuining Qiu Jing Liu Yan Liu Honglian Li Lei Zhang Hong Dong Lixin Sun Song Pan Xiang Zhou Zhe Tian Wenjie Zhang Ruhong Wu Hongsan Sun Yu Huang Xiaohong Su Yongwei Zhang Rui Shen Diankun Chen Guangyuan Wei Yixing Chen Jinqing Peng 2022Building Simulation2022,15,11:5
6High sensitive detection of presenilin-1 point mutation based on electrochemilumi-nescence显示文摘Electrochemiluminescence (ECL) is a high- sensitive detection method with broad biological applications. Ruthenium (Ⅱ) tris (bipyridyl) 2+3(Ru(bpy)) and tripro-pylamine (TPA) are most commomly used combination of the reactive species in ECL. The redox of 2+3Ru(bpy) with excess TPA at the surface of an electrode produces a highly efficient and stable light emission due to a rapid cycle of the reactions. This rapid, highly sensitive and accurate method has been applied to gene quantification. In this work, an ECL detection system is designed and applied in detection of presenilin-1 (PS-1) gene mutation. The results show that given the same polymerase chain reaction (PCR) cycle num-ber, the ECL intensities from the digested and the undigested wild-type samples are nearly identical, but the difference in ECL intensities between the digested and the undigested mutant samples is distinctive. This detection technology connects PCR with ECL and allows a reliable discrimination between the wild-type and the mutant genes.ZHU Debin, XING Da, SHEN Xingyan & YAN Guihong Institute of Laser Life Science, South China Normal University, Guang-zhou 510631, China Correspondence should be addressed to Xing Da (e-mail: xingda@ scnu.edu.cn) 2003Chinese Science Bulletin2003,48,16:4
7Bubble Strategy—A Practical Solution to Return to Regular Life in the Intertwined Era of Vaccine Rollouts and Virus Mutation显示文摘For a long time,vaccination and herd immunity were considered to be the magic solution for controlling coronavirus disease 2019(COVID-19).However,the emergence of new variants altered people’s expectations and prolonged the pandemic duration,especially the Omicron strain with substantially increased transmissibility and decreased vaccine efficacy.Therefore,we are in urgent need of a practical solution to resume regular life to some extent,while mitigating COVID-19 risks in the tight race between vaccine rollouts and virus variation.This commentary proposed that bubble strategy(or closed loop management),utilized in Tokyo 2020 Olympic Games and to be implemented in Beijing 2022 Winter Games,could serve as a novel technique of nonpharmaceutical interventions in coping with such a situation.Ying Shen Da Huo Tong Yu Quanyi Wang 2022China CDC weekly2022,4,4:4
8The First Outbreak of Omicron Subvariant BA.5.2—Beijing Municipality,China,July 4,2022显示文摘On July 4,2022,the first case of Omicron subvariant BA.5.2 in Beijing Municipality was discovered in Yanqing District.The case was a 49-yearold Chinese male who had arrived in Shanghai Municipality via international flight DL9927 from North Carolina,U.S.on June 15.He stayed in a hotel for the 14-day arrival quarantine and was discharged on June 30.He arrived in Beijing via domestic flight MU5103 on July 1 and was transferred point-to-point from the airport to his residence community in Yanqing District.On July 3,his sample was collected through community mass screening and reported positive in the next morning.The case had received 3 doses of Moderna’s mRNA vaccines in the U.S.,with the last shot on May 26,2022.Zhaomin Feng Ying Shen Shuang Li Jia Li Shaohua Wang Zhenquan Zhang Yunkui Shen Fu Li Yang Pan Quanyi Wang Da Huo 2022China CDC weekly2022,4,30:4
9An Efficient Network-on-Chip Router for Dataflow Architecture显示文摘Dataflow 建筑学在许多高效的计算盒子中显示出它的优点。在 dataflow 计算,大量数据经常在通过 network-on-chip (NoC ) 处理元素之中被转移。因此,路由器设计在 dataflow 建筑学的性能上有重要影响。普通路由器为控制流动多核心建筑学被设计,我们发现他们不对 dataflow 建筑学合适。在这个工作,我们在 dataflow 建筑学的 NoCs 分析并且提取数据转移的特征:多重目的地,高注射率,和对延期敏感的性能。基于三个特征,我们为 dataflow 建筑学建议一个新奇、有效的 NoC 路由器。建议路由器支持多目的地;因此,它能在单个转移与多重目的地转移数据。而且,路由器采用输出缓冲区最大化产量并且采用非迁徙包最小化转移延期。试验性的结果证明建议路由器能在一个最先进的路由器上由 3.6x 改进 dataflow 建筑学的性能。Xiao-Wei Shen Xiao-Chun Ye Xu Tan Da Wang Lunkai Zhang Wen-Ming Li Zhi-Min Zhang Dong-Rui Fan Ning-Hui Sun 2017Journal of Computer Science & Technology2017,32,1:4
10A multiplex microsatellite PCR method for evaluating genetic diversity in grass carp(Ctenopharyngodon idellus)显示文摘Grass carp is one of the most important cultured fishes all over the world.The genetic diversity of grass carp plays an important role whatever in selective breeding progress or ecological conservation purposes.However,some genetic diversity researches were not accuracy and cannot be compared with each other due to different molecular markers,sample size and detection methods.In this study,we constructed five multiplex PCR assays contained 20 microsatellites with highly polymorphism and heterozygosity for evaluating genetic information of grass carp.We used nine cultured populations consisting of 507 individuals to detect stability of the five multiplex PCR assays.The results showed that the number of alleles(Na),effective number of alleles(Ne),observed heterozygosity(Ho),expected heterozygosity(He)and polymorphism information content(PIC)of the 20 microsatellite loci were relative high compared with the genetic diversity parameters of microsatellite loci developed by other researchers.Six loci were significantly deviated from Hardy-Weinberg equilibrium(P<0.01).And with exception of the Shaoguan,Indian and Nepal cultured population,all other cultured populations showed very high genetic diversity.Through the test of grass carp populations,we developed an effective and accurate multiplex SSR-PCR assays that can be as statistical powerful tool for detecting genetic information of grass carp.Da Li Shentong Wang Yubang Shen Xinzhan Meng Xiaoyan Xu Rongquan Wang Jiale Li 2018Aquaculture and Fisheries2018,3,6:4
11Value of low-dose and optimized-length computed tomography(CT)scan in CT-guided percutaneous transthoracic needle biopsy of pulmonary nodules显示文摘Objective:To investigate the value of application of low-dose and optimized length CT scan on puncture results,complications and patients’radiation dosage during CT-guided percutaneous biopsy of pulmonary nodules(PTNB).Methods:A total of 231 patients with PTNB under CT guidance were collected.Low dose scanning utilized tube current of 20 mA as compared with 40 mA in conventional dosage.Optimized length in CT is defined as intentionally narrowing the range of CT scanning just to cover 25 mm(5 layers)around the target layer during needle adjustment.According to whether low-dose scans and optimized length scans techniques were utilized,patients were divided into three groups:conventional group(conventional sequence+no optimization),optimized length group(conventional sequence+optimized length),and low-dose optimized length group(low dose sequence+optimized length).The ED(effective dose),the DLP(dose length product),the average CTDIvol(Volume CT dose index),total milliampere second between subgroups were compared.Results:Compared with the conventional group,ED,intraoperative guidance DLP,total milliseconds and operation time in the optimized length group were reduced by 18.2%(P=0.01),37%(P=0.003),17.5%(P=0.013)and13.3%(P=0.021)respectively.Compared with the optimized length group,the ED was reduced by 87%,preoperative positioning,intraoperative guidance and postoperative review DLP were also reduced by 88%,total milliampere second was reduced by 79%,with an average CTDIvol was reduced by 86%,in the low-dose optimized length group(P<0.001 for all).Conclusion:Optimizing the length during CT scanning can effectively reduce the intraoperative radiation dose and reduce the operation time compared with conventional plan;low-dose and optimized length CT scan can further reduce the total radiation dose compared with optimized length group with no differences on intraoperative complications,biopsy results and operation time.Hui Yuan Da Li Yan Zhang Xiaozhen Xie Lujun Shen 2021Journal of Interventional Medicine2021,4,3:4
12经自然腔道取标本手术(NOSES)治疗结直肠癌的国际共识显示文摘近年来,经自然腔道取标本手术(NOSES)治疗结直肠癌已引起了广泛关注。NOSES应用于结直肠手术的潜在优势包括减轻术后疼痛,减少伤口并发症,减少对术后镇痛的需求,加快肠道功能的恢复,缩短住院时间,更好的美容效果,以及给患者带来了更佳的心理状态。尽管NOSES的手术创伤显著减轻,然而该技术也存在一些潜在问题。尤其随着这项新技术的兴起,人们对其无菌的把控、肿瘤学结果和病例选择等问题表示出关切。因此,迫切需要一部操作指南来规范NOSES手术在结直肠癌中的开展。经过国际NOSES联盟全体成员的三轮讨论,最终形成了该部共识。这一共识有助于NOSES在全球范围内的长远发展。Xu Guan Zheng Liu Antonio Longo Jian-Chun Cai William Tzu-Liang Chen Lu-Chuan Chen Ho-Kyung Chun Joaquim Manuel da Costa Pereira Sergey Efetov Ricardo Escalante Qing-Si He Jun-Hong Hu Cuneyt Kayaalp Seon-Hahn Kim Jim S.Khan Li-Jen Kuo Atsushi Nishimura Fernanda Nogueira Junji Okuda Avanish Saklani Ali A.Shafik Ming-Yin Shen Jung-Tack Son Jun-Min Song Dong-Hui Sun Keisuke Uehara Gui-Yu Wang Ye Wei Zhi-Guo Xiong Hong-Liang Yao Gang Yu Shao-Jun Yu Hai-Tao Zhou Suk-Hwan Lee Petr V.Tsarkov Chuan-Gang Fu Xi-Shan Wang The International Alliance of NOSES 2019Gastroenterology Report2019,7,1:3
13A Non-Stop Double Buffering Mechanism for Dataflow Architecture显示文摘两倍缓冲是有效机制隐藏在在薄片上和离开薄片记忆之间的数据转移的潜伏。在 dataflow 建筑学,因为 dataflow 加速器的重复充满并且排干,然而,交换二在许多瓦减少的执行期间缓冲性能。在这个工作,我们为 dataflow 建筑学建议连续双的缓冲机制。没有停止通过在 dataflow 建筑学优化控制逻辑处理元素的执行,建议不停的机制把瓦分到处理元素数组。而且,我们建议一个工作流节目与连续双的缓冲机制合作。在控制逻辑上并且在工作流节目上的优化以后,充满并且排干数组需要越过属于一样的 dataflow 图的所有瓦的执行被做仅仅一次。试验性的结果证明没有优化,为 dataflow 建筑学的建议双缓冲机制在那上完成 16.2% 平均效率改进。Xu Tan Xiao-Wei Shen Xiao-Chun Ye Da Wang Dong-Rui Fan Lunkai Zhang Wen-Ming Li Zhi-Min Zhang Zhi-Min Tang 2018Journal of Computer Science & Technology2018,33,1:3
14Polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) emissions from electric arc furnaces for steelmaking显示文摘The proportion of steel produced using electric arc furnaces(EAFs)is increasing.In this study,polychlorinated dibenzo-p-dioxins and dibenzofurans(PCDD/Fs)concentrations and patterns in stack gas samples from three typical EAFs in steelmaking plants were determined to allow the contributions of EAFs in steelmaking plants to PCDD/Fs emissions in China to be assessed.Samples collection was accomplished by adopting the automatic isokinetic sampling technique,then the PCDD/Fs congeners concentrations were analyzed qualitatively and quantitatively by isotope dilution high-resolution gas chromatography high-resolution mass spectrometry(HRGC-HRMS).The mean PCDD/Fs mass concentrations in stack gases emitted during the scrap preheating phase of plants E1,E2,and E3 were 0.48,385.55,and 0.34 ng Nm^-3,respectively,and the total toxic equivalent(WHO2005-TEQ)concentrations were 0.05,33.16,and 0.03 ng Nm^-3,respectively.The PCDD/Fs mass concentration in stack gases emitted during the melting phase at plant E3 was 0.12 ng Nm^-3(0.02 ng WHO2005-TEQ Nm^-3).The operational temperature of the bag filter of the preheating in the E2 EAF was as high as 230℃,which is conducive to the generation of PCDD/Fs,whether the temperature in the bag filter of E1(150℃)and E3(120℃)are not.Moreover,the bag filter temperature is suggested to be best controlled to below 150℃.The emission factors(EF)for PCDD/Fs released into the air during the scrap preheating phase for plants E1,E2,and E3 were 0.13,52,and 0.10 mg WHO2005-TEQ t1,respectively,and the EF for the smelting phase from plant E3 was 0.14 mg WHO2005-TEQ t1.The emission factor in E2 is 5 times higher than the highest current UNEP toolkit factor for EAFs(10 mg TEQ/tonne steel).Therefore,an update for the UNEP toolkit emission factor for a few case plants is suggested.Several congeners,including 2,3,7,8-TeCDF,1,2,3,4,6,7,8-HpCDF,and 1,2,3,4,6,7,8-HpCDD,were dominant during the preheating phase,while 2,3,7,8-TeCDF,1,2,3,7,8-PeCDF,and 2,3,4,7,8-PeCDF were dominant during the smelting phase.The monitoring data from the case plants helps to better understand the PCDD/Fs emissions of EAFs using preheating process stages.However,a more complete emission inventory requires more case investigations on the PCDD/Fs emissions from representative EAFs steelmaking plants.Qiuting Yang Lili Yang Jia Shen Yuanping Yang Minxiang Wang Xiaoyun Liu Xuejing Shen Changliang Li Jia Xu Fengfeng Li Da Li Guorui Liu Minghui Zheng 2020Emerging Contaminants2020,6,1:3
15Photo-promoted carbonylation of chloroalkanes with carbon monoxide by non-precious metal catalysts显示文摘The photo-promoted carbonylation of chloroalkanes with carbon monoxide was carried out under ambient conditions with copper and cadmium salts catalysts. The results indicated that the corresponding esters were produced with three salt catalysts, i.e. CuBr2, CuCl2 and CdI2. Among these catalysts, CdI2 was the most efficient in terms of ester yield and selectivity, particularly, 60% yield and 75% selectivity in the carbonylation of chlorocyclohexane were achieved. Furthermore, the yield and selectivity of the ester can be improved greatly by adding tri-n-butylamine in the CuBr2 and CuCl2 catalyst systems. On the other hand, the carbonylation did not proceed with single CdCl2, however, the catalytic activity of CdCl2 was increased greatly with NaIá2H2O as additive. As a result, we suggest that iodide ion plays an important role in the catalyst system of the cadmium salts.Hong Wei Wang Ying Ping Jia Da Bin Gao Jing Mei Yin Guang-Yun-Zhou Shen Min Li 2007Chinese Chemical Letters2007,18,7:3
16A New Anti-Collision Algorithm for Multi-Target Real Time Recognitionin RFID System显示文摘The problem of how to distinguish and recognize more than one target when they are entering the working area of the Radio Frequency Identification( RFID ) system should be considered in design of the system. To avoid signal interference with each other among all targets, an anti collision algorithm as well as the condition of working stability is proposed. The computer simulation shows that the new algorithm will be of advantages in the process of system design.SHEN Yu chao, WANG Hai bo, SHEN Shu qun, XU Da xiong (School of Electronic Engineering,Beijing University of Posts and Telecommunications,Beijing 100876,P.R.China) 2000The Journal of China Universities of Posts and Telecommunications2000,7,3:3
17Role of the microenvironment in hepatocellular carcinoma development and progression显示文摘Sheng-Di Wu Yu-Shui Ma Ying Fang Li-Li Liu Da Fu Xi-Zhong Shen 2011Cancer Treatment Reviews2011,,3:3
18Genetic control of compound leaf development in the mungbean(Vigna radiata L.)显示文摘Many studies suggest that there are distinct regulatory processes controlling compound leaf development in different clades of legumes.Loss of function of the LEAFY(LFY)orthologs results in a reduction of leaf complexity to different degrees in inverted repeat-lacking clade(IRLC)and non-IRLC species.To further understand the role of LFY orthologs and the molecular mechanism in compound leaf development in non-IRLC plants,we studied leaf development in unifoliate leaf(un)mutant,a classical mutant of mungbean(Vigna radiata L.),which showed a complete conversion of compound leaves into simple leaves.Our analysis revealed that UN encoded the mungbean LFY ortholog(VrLFY)and played a significant role in leaf development.In situ RNA hybridization results showed that STM-like KNOXI genes were expressed in compound leaf primordia in mungbean.Furthermore,increased leaflet number in heptafoliate leaflets1(hel1)mutants was demonstrated to depend on the function of VrLFY and KNOXI genes in mungbean.Our results suggested that HEL1 is a key factor coordinating distinct processes in the control of compound leaf development in mungbean and its related non-IRLC legumes.Keyuan Jiao Xin Li Shihao Su Wuxiu Guo Yafang Guo Yining Guan Zhubing Hu Zhenguo Shen Da Luo 2019Horticulture Research2019,6,1:2
19Understanding the Percolation Effect in Triboelectric Nanogenerator with Conductive Intermediate Layer显示文摘Introducing the conductive intermediate layer into a triboelectric nanogenerator(TENG)has been proved as an efficient way to enhance the surface charge density that is attributed to the enhancement of the dielectric permittivity.However,far too little attention has been paid to the companion percolation,another key element to affect the output.Here,the TENG with MXeneembedded polyvinylidene fluoride(PVDF)composite film is fabricated,and the dependence of the output capability on the MXene loading is investigated experimentally and theoretically.Specifically,the surface charge density mainly depends on the dielectric permittivity at lower MXene loadings,and in contrast,the percolation becomes the degrading factor with the further increase of the conductive loadings.At the balance between the dielectric and percolation properties,the surface charge density of the MXene-modified TENG obtained 350%enhancement compared to that with the pure PVDF.This work shed new light on understanding the dielectric and percolation effect in TENG,which renders a universal strategy for the high-performance triboelectronics.Binbin Zhang Guo Tian Da Xiong Tao Yang Fengjun Chun Shen Zhong Zhiming Lin Wen Li Weiqing Yang 2021Research2021,,1:2
20Quantitative Analysis of Eight Ginsenosides in Red Ginseng Using Ginsenoside Rg1 as Single Reference Standard显示文摘Objective:To develop a reversed-phase high-performance liquid chromatography method for the quantification of major ginsenosides in red ginseng(RG,the steamed and dried root of the cultivar of Panax ginseng C.A.Mey).Methods:A feasible method was developed in strict accordance with chromatographic properties of eight ginsenosides.Their contents could be unveiled with conventional external standard method,or as an alternative,using ginsenoside Rg1 as the single reference standard by means of seven conversion factors.Those parameters had been validated on different chromatographic columns and instruments.Results:Twenty-one batches of RG samples were determined.In addition,the chromatograms of RG and confusing species,including Panax ginseng,Panax quinquefolium,and Panax notoginseng,were apparently different.Conclusions:The method was proved to be efficient for the quality control of RG.Juan Da Qiu-Rong Wang Yan Wang Shuai Yao Yong Huang Wen-Long Wei Jian Liang Yao Shen Gerhard Franz De-An Guo 2021World Journal of Traditional Chinese Medicine2021,7,1:2
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