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| 1 | RAPD Analysis of M_1 Generation of Gladiolus hybridus Hort Treated by EMS显示文摘Gladiolus hybridus Hort is one of the world's famous cutting flowers. It is very popular because of the big size, bright color, various shape, and long period of bloom. New species should be cultivated in order to meet the consumers' need of asking for the new. Among the technologies of cultivating new species of flowers, mutagenic breeding is a shortcut. This study treated corm bud of G. hybridus Hort with EMS of different consistency. Then M1 after treated was analyzed by RAPD. The result showed that EMS was a very effective mutagenic agent for the corm bud of G. hybridus Hort. With the increase of consistency, the mutagenic range increased first, then decreased, among which 0.6% EMS treatment had the biggest influence. However, with the same EMS consistency, there was not close relevancy between the amount of mutagenic agent and the divergence of plant's genomes, which offered a molecular basis for selecting plants with good mutation. | WANG Jingang GUO Ying CHE Daidi LIU Shenkui YANG Chuanpin | 2009 | Journal of Northeast Agricultural University(English Edition)2009，16，1： | 6 |
| 2 | High efficiency production of ginsenoside compound K by catalyzing ginsenoside Rb1 using snailase显示文摘The rare ginsenoside Compound K(C-K) is attracting more attention because of its good physiological activity and urgent need. There are many pathways to obtain ginsenoside C-K, including chemical and biological methods. Among these, the conversion of PPD-type ginsenosides by enzymatic hydrolysis is a trend due to its high efficiency and mild conditions. For effectively extracting from the other panaxadiol saponins, the conversion process for ginsenoside C-K was investigated using snailases in this study. The univariate experimental design and response surface methodology were used to determine the optimal hydrolysis conditions for the conversion of ginsenoside Rb1 into ginsenoside C-K by snailases. The optimum conditions were as follows: p H 5.12, temperature 51 °C, ratio of snailase/substrate 0.21, and reaction time 48 h. On the basis of these parameters, the addition of 1.0 mmol·L^(-1) ferric ion was found to significantly improve the enzymolysis of snailases for the first time. With the above conditions, the maximum conversion rate reached 89.7%, suggesting that the process can obviously increase the yield of ginsenoside C-K. The bioassay tests indicated that the ginsenoside C-K showed anti-tumor activity in a series of tumor cell lines. Based on these results, we can conclude that the process of rare ginsenoside CK production by enzymolysis with snailase is feasible, efficient, and suitable for the industrial production and application. | Zhiguang Duan Chenhui Zhu Jingjing Shi Daidi Fan Jianjun Deng Rongzhan Fu Rong Huang Cuiying Fan | 2018 | Chinese Journal of Chemical Engineering2018，26，7： | 5 |
| 3 | A biomimetic bi-layered tissue engineering scaffolds for osteochondral defects repair显示文摘Osteochondral defects are most commonly characterized by damages to both cartilage and underlying subchondral bone tissues,thus developing bi-layered scaffold that can concurrently regenerate these two specific lineages becomes challenge.In this study,the highly biomimetic bi-layered scaffolds were successfully prepared using human-like-collagen(HLC),hyaluronan(HA)and nano hydroxyapatite(HAP)particles,combined with'liquid phase synthesis'technology,freeze-drying and chemical crosslinking techniques,which was simulated the composition of natural extracellular matrix to repair osteochondral defects.This novel bilayer osteochondral graft had a seamlessly integrated layer structure,suitable pore size,high levels of porosity,and excellent mechanical properties.In vitro cell experiments of the bilayer scaffold indicated that the scaffold could promote the proliferation and adhesion of human bone marrow mesenchymal stem cells.In vivo osteochondral defects and micro-CT experiment revealed that bilayer scaffolds showed complete closure of the defect.Histology confirmed collagen and glycosaminoglycans were deposited in the new matrix of hyaline cartilage and bone in the bilayer scaffold group.Therefore,the developed bionic bilayer scaffold enhanced the regeneration of hyaline cartilage through subchondral bone formation and lateral host-tissue integration.In conclusion,this bilayer scaffold based on HLC could be used as the desired strategy for osteochondral defects regeneration. | LIU KaiQiang LIU YanNan DUAN ZhiGuang MA XiaoXuan FAN DaiDi | 2021 | Science China(Technological Sciences)2021，64，4： | 4 |
| 4 | Glucose and MMP-9 dual-responsive hydrogel with temperature sensitive self-adaptive shape and controlled drug release accelerates diabetic wound healing显示文摘Chronic diabetic wounds are an important healthcare challenge. High concentration glucose, high level of matrix metalloproteinase-9 (MMP-9), and long-term inflammation constitute the special wound environment of diabetic wounds. Tissue necrosis aggravates the formation of irregular wounds. All the above factors hinder the healing of chronic diabetic wounds. To solve these issues, a glucose and MMP-9 dual-response temperature-sensitive shape self-adaptive hydrogel (CBP/GMs@Cel&INS) was designed and constructed with polyvinyl alcohol (PVA) and chitosan grafted with phenylboric acid (CS-BA) by encapsulating insulin (INS) and gelatin microspheres con-taining celecoxib (GMs@Cel). Temperature-sensitive self-adaptive CBP/GMs@Cel&INS provides a new way to balance the fluid-like mobility (self-adapt to deep wounds quickly, approximately 37 ◦C) and solid-like elasticity (protect wounds against external forces, approximately 25 ◦C) of self-adaptive hydrogels, while simultaneously releasing insulin and celecoxib on-demand in the environment of high-level glucose and MMP-9. Moreover, CBP/ GMs@Cel&INS exhibits remodeling and self-healing properties, enhanced adhesion strength (39.65 ± 6.58 kPa), down-regulates MMP-9, and promotes cell proliferation, migration, and glucose consumption. In diabetic full-thickness skin defect models, CBP/GMs@Cel&INS significantly alleviates inflammation and regulates the local high-level glucose and MMP-9 in the wounds, and promotes wound healing effectively through the synergistic effect of temperature-sensitive shape-adaptive character and the dual-responsive system. | Wanyi Zhou Zhiguang Duan Jing Zhao Rongzhan Fu Chenhui Zhu Daidi Fan | 2022 | Bioactive Materials2022，7，11： | 4 |
| 5 | Recent advances in systemic and local delivery of ginsenosides using nanoparticles and nanofibers显示文摘Ginsenosides are the main pharmacologically active constituents of ginseng which have been used in East Asian countries for centuries to modulate blood pressure,metabolism and immune function.Following the technological advances in isolation,purification and mass production,their mechanisms of action are gradually elucidated,providing solid basis for clinical applications.Ginseng extracts(total ginsenosides)and ginsenoside Rg3,CK,Rd have been marketed or entered clinical trials as drugs or dietary supplements.Despite the proven safety and efficacy of some ginsenosides,their applications are hindered by inferior pharmacokinetics such as low solubility,poor membrane permeability and metabolic instability.Nanoparticle formulation of drugs and implantable drug depots are effective strategies to improve the pharmacokinetics of therapeutic agents by enhancing solubility,providing protection,facilitating intracellular transport,and enabling sustained and controlled release.This mini-review summarizes the recent advances in systemic delivery of ginsenosides using liposomes,micelles,albumin-based nanoparticles,and inorganic nanoparticles,as well as local delivery of ginsenosides by electronspun fibrous membranes and hydrogels. | Jing Zhao Zhiguang Duan Xiaoxuan Ma Yannan Liu Daidi Fan | 2021 | Chinese Journal of Chemical Engineering2021，34，2： | 3 |
| 6 | Molecular design, synthesis strategies and recent advances of hydrogels for wound dressing applications显示文摘With the changes in the modern disease spectrum,pressure ulcers,diabetic feet,and vascular-derived diseases caused refractory wounds is increasing rapidly.The development of wound dressings has partly improved the effect of wound management.However,traditional wound dressings can only cover the wound and block bacteria,but are generally powerless to recurrent wound infection and tissue healing.There is an urgent need to develop a new type of wound dressing with comprehensive performance to achieve multiple effects such as protecting the wound site from the external environment,absorbing wound exudate,anti-inflammatory,antibacterial,and accelerating wound healing process.Hydrogel wound dressings have the aforementioned characteristics,and can keep the wound in a moist environment because of the high water content,which is an ideal choice for wound treatment.This review introduces the wound healing process and the development and performance advantages of hydrogel wound dressings.The choice of different preparation materials gives the particularities of different hydrogel wound dressings.It also systematically explains the main physical and chemical crosslinking methods for hydrogel synthesis.Besides,in-depth discussion of four typical hydrogel wound dressings including double network hydrogels,nanocomposite hydrogels,drug-loaded hydrogels and smart hydrogels fully demonstrates the feasibility of developing hydrogels as wound dressing products and their future development trends. | Dan Zeng Shihong Shen Daidi Fan | 2021 | Chinese Journal of Chemical Engineering2021，34，2： | 3 |
| 7 | Cloning and Analysis of Full-Length cDNA of PumNPR1 Gene from Pyrus ussuriensis Maxim显示文摘The purpose of this study is to find a new gene resource for the researches of molecular breeding of Rosaceae plants disease-resistance.Pyrus ussuriensis Maxim is used as a starting material to clone the full-length cDNA of NPR1(nonexpressor of pathogenesis-related genes 1) which is a key regulator in SA(salicylic acid)-mediated systemic acquired resistance(SAR) by homologous cloning and RACE techniques.The length of the cDNA sequence was 1 767 bp,the ORF was 1 761 bp,it coded 586 amino acids,pI=5.58,the relative molecular weight was 65.009 ku,contained 19 kinds of amino acids,and had full BTB/POZ and ANK domains.Compared the homology of NPR1 gene in GenBank database,the homology with Pyrus pyrifolia,Arabidopsis thaliana,Nicotiana tabacum,Lycopersicon esculentum,Oryza sativa,Helianthus annuus were 98%,62%,68%,65%,57%,63%.The homology of functional area were 99%,78%,82%,79%,74%,77%.This NPR1 gene was considered as homologic gene of Pyrus ussuriensis Maxim and named PumNPR1. | CHE Daidi FAN Jinping WANG Jingang XU Ping YANG Tao LIU Shenkui | 2008 | Journal of Northeast Agricultural University(English Edition)2008，15，2： | 2 |
| 8 | Research on protoplast preparation and culture in Gladiolus hybridus Hort显示文摘Protoplasts prepared from the aseptic leaves of Rose Supreme in Gladiolus hybridus Hort. were cultured. The yield of protoplasts with vigour was the highest under the following conditions: 1.5% cellulase Onzuka RS, 0.5% pectinase Y-23, 0.6 mol· L^(-1) mannitol, pH 5.8, digestion time 2 h, and temperature 27℃. After isolation and purification, protoplasts were cultured in solid and liquid combined medium, which included KM8P, 0.6 mol· L^(-1) mannitol, 1.0 mg· L^(-1) 2, 4-D, 0.2 mg· L^(-1) NAA and 0.2 mg· L^(-1) KT. The division of the protoplasts first emerged in medium after cultured 4 days, and emerged only a few times. | ZHANG Ying GONG Sufang WANG Jingang CHE Daidi | 2007 | Journal of Northeast Agricultural University(English Edition)2007，14，1： | 2 |
| 9 | A temperature responsive adhesive hydrogel for fabrication of flexible electronic sensors显示文摘Flexible electronics are playing an increasingly important role in human health monitoring and healthcare diagnosis.Strong adhesion on human tissue would be ideal for reducing interface resistance and motion artifacts,but arising problems such as skin irritation,rubefaction,and pain upon device removal have hampered their utility.Here,inspired by the temperature reversibility of hydrogen bonding,a skin-friendly conductive hydrogel with multiple-hydrogen bonds was designed by using biocompatible poly(vinyl alcohol)(PVA),phytic acid(PA),and gelatin(Gel).The obtained PVA/PA/Gel(PPG)hydrogel with temperature-triggered tunable mechanic could reliably adhere to skin and detect electrophysiological signals under a hot compress while be readily removed under a cool compress.Furthermore,the additional advantages of transparency,breathability,and antimicrobial activity of the PPG hydrogel ensure its long-time wearable value on the skin.It is both environmentally friendly and cost saving for the waste PPG hydrogel during production can be recycled based on their reversible physical bonding.The PPG hydrogel sensor is expected to have good application prospects to record electrophysiological signals in human health monitoring. | Wan Liu Ruijie Xie Jingyu Zhu Jiansheng Wu Junfeng Hui Xiaoyan Zheng Fengwei Huo Daidi Fan | 2022 | npj Flexible Electronics2022，6，1： | 2 |
| 10 | Construction of multifunctional hydrogel based on the tannic acid-metal coating decorated MoS2 dual nanozyme for bacteria-infected wound healing显示文摘Bacterial infection,tissue hypoxia and inflammatory response can hinder the infected wound repair process.To mitigate the above issues,tannic acid-chelated Fe-decorated molybdenum disulfide nanosheets(MoS2@TA/Fe NSs)with dual enzyme activities were developed and anchored to a multifunctional hydrogel.The hydrogel exhibited excellent antibacterial ability owing to the combined effects of photothermal therapy(PTT),glutathione(GSH)loss,and the peroxidase(POD)-like activity(catalyse H2O_(2)into⋅OH under acid condition)of MoS2@TA/Fe NSs.Benefitting from the catalase(CAT)-like activity,the hydrogel could decompose H2O_(2)into O_(2)at neutral pH to relieve hypoxia and supply adequate O_(2).POD-like activity was mainly attributed to MoS2 NSs,while CAT-like activity was primarily due to TA/Fe complex.Moreover,MoS2@TA/Fe NSs endowed the hydrogel with outstanding anti-oxidant ability to scavenge redundant reactive oxygen species(ROS)and reactive nitrogen species(RNS)under neutral environment to maintain the balance of antioxidant systems and prevent inflammation.In addition,the hydrogel could inhibit the release of inflammatory factors for the anti-inflammatory property of TA.TA retained partial phenolic hydroxyl groups,which cross-linked the nanosheets to the network structure of the hydrogel and promoted the adhesion of hydrogels.Due to the dynamic boron ester bonds between polyvinyl alcohol(PVA),dextran(Dex),MoS2@TA/Fe,and borax,the hydrogel demonstrated fast self-healing and rapid shape adaptability.This shape-adaptable adhesive hydrogel could fill the whole wound and closely contact the wound,ensuring that it achieved its functions with maximum efficiency.The MoS2@TA/Fe nanozyme-anchored multifunctional hydrogel showed high potential for bacteria-infected wound healing. | Yang Li Rongzhan Fu Zhiguang Duan Chenhui Zhu Daidi Fan | 2022 | Bioactive Materials2022，7，3： | 2 |
| 11 | Effect of Temperature on Self-Interaction of Human-Like Collagen显示文摘似人类的骨胶原(HLC ) 的自我相互作用上的温度的效果被恐水病的相互作用调查层析, calorimetric 测量,和钠 dodecyl sulphate-polyacrylamide 胶化电气泳动(SDS 页) 分析。结果证明相互作用角色的三种类型可以在 350 点在 HLC | Fan, Daidi Xing, Jianyu Xue, Wenjiao Zhu, Chenhui Ma, Xiaoxuan Ma, Rong | 2011 | Chinese Journal of Chemistry2011，29，9： | 2 |
| 12 | 3D simplified finite el- ements analysis of load and contact angle in a slewing ball bearing 显示文摘 | DAIDIE A CHAIB Z GHOSN A | 2008 | Journal of Mechanical Design2008，130，08： | 1 |
| 13 | 3D simplified finite elements analysis of load and contact angle in a slewing ball bearing 显示文摘 | Daidie A Chaib Z Ghosn A | 2008 | Journal of Mechanical Design2008，130，8： | 1 |
| 14 | Screw Behavior in Large Diameter Slewing Bearing Assemblies:Numerical and Experimental Analyses 显示文摘 | Chaib Zouhalr Daidie Alain Leray Dimltrl | 2007 | International Journal on Interactive Design and Manufacturing2007，1，1： | 1 |
| 15 | Temperature-dependent self-assembly of near-infrared (NIR) luminescent Zn 2 Ln and Zn 2 Ln 3 (Ln=Nd, Yb or Er) complexes from the flexible Salen-type Schiff-base ligand显示文摘 | Tiezheng Miao Zhao Zhang Weixu Feng Peiyang Su Heini Feng Xingqiang Lü Daidi Fan Wai-Kwok Wong Richard A. Jones Chengyong Su | 2014 | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy2014，，： | 1 |
| 16 | 3D simplified finite elements analysis of load and contact angle in a slewing ball bearing显示文摘 | Alain Daidie Zouhair Chaib Antoine Ghosn | 2008 | Journal of Mechanical Design2008，，130： | 1 |
| 17 | Screw Behavior in Large Diameter Slewing Bearing Assemblies: Numerical and Experimental Analysis 显示文摘 | Chaib Z Daidie A Leray D | 2007 | International Journal on In- teractive Design and Manufacturing2007，1，1： | 1 |
| 18 | Preparation and electrochemical lithium storage features of TiO 2 hollow spheres显示文摘 | Liang Xiao Minglei Cao Daidi Mei Yonglin Guo Lifeng Yao Deyu Qu Bohua Deng | 2013 | Journal of Power Sources2013，，： | 1 |
| 19 | Equivalent Axial Stiffness of Various Components in Bolted Joints Subjected to Ax- ial Loading显示文摘 | Feras Alkatan Pierre Stephan Alain Daidie | 2007 | Finite Elements in Analysis and Design2007，43，： | 1 |
| 20 | Equivalent axial stiffness of various components in bolted joints subjected to axial loading 显示文摘 | Alkatan F Stephan P Daidie A | 2007 | Finite Elements in Analysis and Design2007，43，8： | 1 |
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