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| 1 | Cryopreservation for delayed circulating tumor cell isolation is a valid strategy for prognostic association of circulating tumor cells in gastroesophageal cancer显示文摘AIM To demonstrate the feasibility of cryopreservation of peripheral blood mononuclear cells(PBMCs) for prognostic circulating tumor cell(CTC) detection in gastroesophageal cancer.METHODS Using 7.5 m L blood samples collected in EDTA tubes from patients with gastroesopheagal adenocarcinoma, CTCs were isolated by epithelial cell adhesion molecule based immunomagnetic capture using the Iso Flux platform. Paired specimens taken during the same blood draw(n = 15) were used to compare number of CTCs isolated from fresh and cryopreserved PBMCs. Blood samples were processed within 24 h to recover the PBMC fraction, with PBMCs used for fresh analysis immediately processed for CTC isolation. Cryopreservation of PBMCs lasted from 2 wk to 25.2 mo(median 14.6 mo). CTCs isolated from pre-treatment cryopreserved PBMCs(n = 43) were examined for associations with clinicopathological variables and survival outcomes.RESULTS While there was a significant trend to a decrease in CTC numbers associated with cryopreserved specimens(mean number of CTCs 34.4 vs 51.5, P = 0.04), this was predominately in samples with a total CTC count of > 50, with low CTC count samples less affected(P = 0.06). There was no significant association between the duration of cryopreservation and number of CTCs. In cryopreserved PBMCs from patient samples prior to treatment, a high CTC count(> 17) was associated with poorer overall survival(OS)(n = 43, HR = 4.4, 95%CI: 1.7-11.7, P = 0.0013). In multivariate analysis, after controlling for sex, age, stage, ECOG performance status, and primary tumor location, a high CTC count remained significantly associated with a poorer OS(HR = 3.7, 95%CI: 1.2-12.4, P = 0.03). CONCLUSION PBMC cryopreservation for delayed CTC isolation is a valid strategy to assist with sample collection, transporting and processing. | Daniel Brungs David Lynch Alison WS Luk Elahe Minaei Marie Ranson Morteza Aghmesheh Kara L Vine Martin Carolan Mouhannad Jaber Paul de Souza Therese M Becker | 2018 | World Journal of Gastroenterology2018，24，7： | 5 |
| 2 | Acoustofluidic separation enables early diagnosis of traumatic brain injury based on circulating exosomes显示文摘Traumatic brain injury(TBI)is a global cause of morbidity and mortality.Initial management and risk stratification of patients with TBI is made difficult by the relative insensitivity of screening radiographic studies as well as by the absence of a widely available,noninvasive diagnostic biomarker.In particular,a blood-based biomarker assay could provide a quick and minimally invasive process to stratify risk and guide early management strategies in patients with mild TBI(mTBI).Analysis of circulating exosomes allows the potential for rapid and specific identification of tissue injury.By applying acoustofluidic exosome separation—which uses a combination of microfluidics and acoustics to separate bioparticles based on differences in size and acoustic properties—we successfully isolated exosomes from plasma samples obtained from mice after TBI.Acoustofluidic isolation eliminated interference from other blood components,making it possible to detect exosomal biomarkers for TBI via flow cytometry.Flow cytometry analysis indicated that exosomal biomarkers for TBI increase in the first 24 h following head trauma,indicating the potential of using circulating exosomes for the rapid diagnosis of TBI.Elevated levels of TBI biomarkers were only detected in the samples separated via acoustofluidics;no changes were observed in the analysis of the raw plasma sample.This finding demonstrated the necessity of sample purification prior to exosomal biomarker analysis.Since acoustofluidic exosome separation can easily be integrated with downstream analysis methods,it shows great potential for improving early diagnosis and treatment decisions associated with TBI. | Zeyu Wang Haichen Wang Ryan Becker Joseph Rufo Shujie Yang Brian E.Mace Mengxi Wu Jun Zou Daniel T.Laskowitz Tony Jun Huang | 2021 | Microsystems & Nanoengineering2021，7，2： | 3 |
| 3 | Ticagrelor Effects on Myocardial Infarction and the Impact of Event Adjudication in the Platelet Inhibition and Patient Outcomes (PLATO) Trial显示文摘 | Kenneth W. Mahaffey Claes Held Daniel M. Wojdyla Stefan K. James Hugo A. Katus Steen Husted Philippe Gabriel Steg Christopher P. Cannon Richard C. Becker Robert F. Storey Nardev S. Khurmi José C. Nicolau Cheuk-Man Yu Diego Ardissino Andrzej Budaj Joao Mor | 2014 | Journal of the American College of Cardiology2014，，： | 2 |
| 4 | Malignant ascites: Systematic review and guideline for treatment显示文摘 | Gerhild Becker Daniel Galandi Hubert E. Blum | 2005 | European Journal of Cancer2005，，5： | 2 |
| 5 | Heritability of Platelet Responsiveness to Aspirin in Activation Pathways Directly and Indirectly Related to Cyclooxygenase-1显示文摘 | Nauder Faraday Lisa R. Yanek Rasika Mathias J Enrique Herrera-Galeano Dhananjay Vaidya Taryn F. Moy M Daniele Fallin Alexander F. Wilson Paul F. Bray Lewis C. Becker Diane M. Becker | 2007 | Circulation2007，，19： | 1 |
| 6 | Adenocarcinomas of the Esophagogastric Junction Are More Likely to Respond to Preoperative Chemotherapy than Distal Gastric Cancer显示文摘 | Daniel Reim Ralf Gertler Alexander Novotny Karen Becker Christian Büschenfelde Matthias Ebert Martin Dobritz Rupert Langer Heinz Hoefler Helmut Friess Christoph Schumacher | 2012 | Annals of Surgical Oncology2012，，7： | 1 |
| 7 | Double-oblique free-breathing high resolution three-dimensional coronary magnetic resonance angiography显示文摘 | Matthias Stuber René M. Botnar Peter G. Danias Daniel K. Sodickson Kraig V. Kissinger Marc Van Cauteren Jan De Becker Warren J. Manning | 1999 | Journal of the American College of Cardiology1999，，2： | 1 |
| 8 | Online Peer-to-Peer Lending: A Literature Review 显示文摘 | Alexander Bacbmann Alexander Becker Daniel Buerekner | 2011 | Journal of Internet Banking and Commerce2011，16，2： | 1 |
| 9 | Online Peer-to-Peer Lending:A Litera-ture Review显示文摘 | Alexander Bachmann Alexander Becker Daniel Buerckner | | 0，，02： | 1 |
| 10 | What makes local governments’ online communications successful? Insights from a multi-method analysis of Facebook显示文摘 | Sara Hofmann Daniel Beverungen Michael R?ckers J?rg Becker | 2013 | Government Information Quarterly2013，，4： | 1 |
| 11 | Illness perceptions mediate the relationship between depression and quality of life in patients with epilepsy显示文摘 | Amanda J. Shallcross Danielle A. Becker Anuradha Singh Daniel Friedman Jacqueline Montesdeoca Jacqueline French Orrin Devinsky Tanya M. Spruill | 2015 | Epilepsia2015，，： | 1 |
| 12 | Replaybased synchronization of timestamps in event traces of massively parallel applications显示文摘 | Daniel Becker John C Linford Rolf Rabenseifner | 2009 | Scalable Computing:Practice and Experience2009，10，1： | 1 |
| 13 | Trustworthy software systems显示文摘 | Steffen Becker Wilhelm Hasselbring Alexandra Paul Marko Boskovic Heiko Koziolek Jan Ploski Abhishek Dhama Henrik Lipskoch Matthias Rohr Daniel Winteler Simon Giesecke Roland Meyer Mani Swaminathan Jens Happe Margarete Muhle Timo Warns | 2006 | ACM SIGSOFT Software Engineering Notes2006，，6： | 1 |
| 14 | Ticagrelor Compared With Clopidogrel by Geographic Region in the Platelet Inhibition and Patient Outcomes (PLATO) Trial显示文摘 | Kenneth W. Mahaffey Daniel M. Wojdyla Kevin Carroll Richard C. Becker Robert F. Storey Dominick J. Angiolillo Claes Held Christopher P. Cannon Stefan James Karen S. Pieper Jay Horrow Robert A. Harrington Lars Wallentin | 2011 | Circulation2011，，5： | 1 |
| 15 | Outcomes After Cardioversion and Atrial Fibrillation Ablation in Patients Treated With Rivaroxaban and Warfarin in the ROCKET AF Trial显示文摘 | Jonathan P. Piccini Susanna R. Stevens Yuliya Lokhnygina Manesh R. Patel Jonathan L. Halperin Daniel E. Singer Graeme J. Hankey Werner Hacke Richard C. Becker Christopher C. Nessel Kenneth W. Mahaffey Keith A.A. Fox Robert M. Califf Günter Breithardt | 2013 | Journal of the American College of Cardiology2013，，19： | 1 |
| 16 | Smad7 Controls Resistance of Colitogenic T Cells to Regulatory T Cell-Mediated Suppression显示文摘 | Massimo C. Fantini Angelamaria Rizzo Daniele Fina Roberta Caruso Massimiliano Sarra Carmine Stolfi Christoph Becker Thomas T. MacDonald Francesco Pallone Markus F. Neurath Giovanni Monteleone | 2009 | Gastroenterology2009，，4： | 1 |
| 17 | Innovative Machine Concepts for 3D Bending of Tubes and Profiles显示文摘 | Hermes Matthias Staupendahl Daniel Becker Christoph Tekkaya A. Erman | 2011 | Key Engineering Materials2011，，473： | 1 |
| 18 | Trustworthy software systems显示文摘 | Steffen Becker Wilhelm Hasselbring Alexandra Paul Marko Boskovic Heiko Koziolek Jan Ploski Abhishek Dhama Henrik Lipskoch Matthias Rohr Daniel Winteler Simon Giesecke Roland Meyer Mani Swaminathan Jens Happe Margarete Muhle Timo Warns | 2006 | ACM SIGSOFT Software Engineering Notes2006，，6： | 1 |
| 19 | Shifting trends in liver-directed management of patients with colorectal liver metastasis: A population-based analysis显示文摘 | Skye C. Mayo Jennifer E. Heckman Andrew D. Shore Hari Nathan Alexander A. Parikh John F.P. Bridges Robert A. Anders Daniel A. Anaya Natasha S. Becker Timothy M. Pawlik | 2011 | Surgery2011，，2： | 1 |
| 20 | Micro- bial community transcriptomes reveal microbes and metabol- ic pathways associated with dissolved organic matter turnover in the sea显示文摘 | McCarren J Jamie W Becker Daniel J Repeta | 2010 | Proceedings of the National Academy of Sci- ences2010，107，16： | 1 |
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