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| 1 | Approaches to establish Q-markers for the quality standards of traditional Chinese medicines显示文摘Traditional Chinese medicine(TCM) has played a pivotal role in maintaining the health of Chinese people and is now gaining increasing acceptance around the global scope. However, TCM is confronting more and more concerns with respect to its quality. The intrinsic 'multicomponent and multitarget' feature of TCM necessitates the establishment of a unique quality and bioactivity evaluation system, which is different from that of the Western medicine. However, TCM is investigated essentially as'herbal medicine' or 'natural product', and the pharmacopoeia quality monographs are actually chemicalmarkers-based, which can ensure the consistency only in the assigned chemical markers, but, to some extent, have deviated from the basic TCM theory. A concept of 'quality marker'(Q-marker), following the 'property-effect-component' theory, is proposed. The establishment of Q-marker integrates multidisciplinary technologies like natural products chemistry, analytical chemistry, bionics, chemometrics,pharmacology, systems biology, and pharmacodynamics, etc. Q-marker-based fingerprint and multicomponent determination conduce to the construction of more scientific quality control system of TCM.This review delineates the background, definition, and properties of Q-marker, and the associated technologies applied for its establishment. Strategies and approaches for establishing Q-marker-based TCM quality control system are presented and highlighted with a few TCM examples. | Wenzhi Yang Yibei Zhang Wanying Wu Luqi Huang Dean Guo Changxiao Liu | 2017 | Acta Pharmaceutica Sinica B2017，7，4： | 51 |
| 2 | Single-cell transcriptomics reveals regulators underlying immune cell diversity and immune subtypes associated with prognosis in nasopharyngeal carcinoma显示文摘Nasopharyngeal carcinoma(NPC)is an aggressive malignancy with extremely skewed ethnic and geographic distributions.Increasing evidence indicates that targeting the tumor microenvironment(TME)represents a promising therapeutic approach in NPC,highlighting an urgent need to deepen the understanding of the complex NPC TME.Here,we generated single-cell transcriptome profiles for 7581 malignant cells and 40,285 immune cells from fifteen primary NPC tumors and one normal sample.We revealed malignant signatures capturing intratumoral transcriptional heterogeneity and predicting aggressiveness of malignant cells.Diverse immune cell subtypes were identified,including novel subtypes such as CLEC9A^+dendritic cells(DCs).We further revealed transcriptional regulators underlying immune cell diversity,and cell–cell interaction analyses highlighted promising immunotherapeutic targets in NPC.Moreover,we established the immune subtype-specific signatures,and demonstrated that the signatures of macrophages,plasmacytoid dendritic cells(pDCs),CLEC9A^+DCs,natural killer(NK)cells,and plasma cells were significantly associated with improved survival outcomes in NPC.Taken together,our findings represent a unique resource providing in-depth insights into the cellular heterogeneity of NPC TME and highlight potential biomarkers for anticancer treatment and risk stratification,laying a new foundation for precision therapies in NPC. | Yu-Pei Chen Jian-Hua Yin Wen-Fei Li Han-Jie Li Dong-Ping Chen Cui-Juan Zhang Jia-Wei Lv Ya-Qin Wang Xiao-Min Li Jun-Yan Li Pan-Pan Zhang Ying-Qin Li Qing-Mei He Xiao-Jing Yang Yuan Lei Ling-Long Tang Guan-Qun Zhou Yan-Ping Mao Chen Wei Ke-Xu Xiong Hong-Bo Zhang Shi-Da Zhu Yong Hou Ying Sun Michael Dean Ido Amit Kui Wu Dong-Ming Kuang Gui-Bo Li Na Liu Jun Ma | 2020 | Cell Research2020，30，11： | 19 |
| 3 | MicroRNA-143 suppresses gastric cancer cell growth and induces apoptosis by targeting COX-2显示文摘AIM:To investigate the function of microRNA-143(miR-143)in gastric cancer and explore the target genes of miR-143.METHODS:A quantitative real-time reverse transcription-polymerase chain reaction(RT-PCR)analysis was performed to evaluate miR-143 expression in gastric cancer cell lines.After transfecting gastric cancer cells with miR-143-5p and miR-143-3p precursors,Alamar blue and apoptosis assays were used to measure the respective proliferation and apoptosis rates.Cyclooxygenase-2(COX-2)expression was determined by realtime RT-PCR and Western blot assays after miR-143transfection.Reporter plasmids were constructed,and a luciferase reporter assay was used to identify the miR-143 binding site on COX-2.RESULTS:Both miR-143-5p and miR-143-3p were significantly downregulated in multiple gastric cancer cell lines.Forced miR-143-5p and miR-143-3p expression in gastric cancer cells produced a profound cytotoxic effect.MiR-145-5p transfection into gastric cancer cells resulted in a greater growth inhibitory effect(61.23%±3.16%vs 46.58%±4.28%,P<0.05 in the MKN-1cell line)and a higher apoptosis rate(28.74%±1.93%vs 22.13%±3.31%,P<0.05 in the MKN-1 cell line)than miR-143-3p transfection.Further analysis indicated that COX-2 expression was potently suppressed by miR-143-5p but not by miR-143-3p.The activity of a luciferase reporter construct that contained the 3’-untranslated region(UTR)of COX-2 was downregulated by miR-143-5p(43.6%±4.86%,P<0.01)but not by miR-143-3p.A mutation in the miR-145-5p binding site completely ablated the regulatory effect on luciferase activity,which suggests that there is a direct miR-145-5p binding site in the 3’-UTR of COX-2.CONCLUSION:Both miR-143-5p and miR-143-3p function as anti-oncomirs in gastric cancer.However,miR-143-5p alone directly targets COX-2,and it exhibits a stronger tumor suppressive effect than miR-143-3p. | Xiao-Li Wu Bin Cheng Pei-Yuan Li Huan-Jun Huang Qiu Zhao Zi-Li Dan Dean Tian Peng Zhang | 2013 | World Journal of Gastroenterology2013，19，43： | 15 |
| 4 | Nrf2抗氧化损伤通路在CCl_4所致小鼠肝毒性中的保护作用(英文)显示文摘目的观察核转录相关因子2(nuclear factor erythroid 2-related factor 2,Nrf2)抗氧化损伤通路对四氯化碳(CCl4)所致小鼠肝毒性的保护作用。方法选取Nrf2-null、Wild-type、Keap1-KD、Keap1-HKO小鼠,按基因型分为4组,分别给予40 mg/kg CCl4腹腔注射,腹腔注射生理盐水(10 m L/kg)作对照,16 h后收集血浆和肝组织样本,检测血中谷丙转氨酶(alanine transaminase,ALT)和乳酸脱氢酶(lactic dehydrogenase,LDH)活性以及肝组织中丙二醛(malondialdehyde,MDA)含量;HE染色观察肝组织病理学变化,Real-time PCR和Western blot法检测相关基因表达。结果 CCl4增加Nrf2-null组和Wild-type组血中ALT、LDH活性及肝组织MDA含量,同时造成肝组织病理损伤,而在Keap1-KD组和Keap1-HKO组影响不明显;Nrf2因子靶基因(Nqo1和Gclc)在CCl4的诱导表达逐渐增高(顺序为Nrf2-null、Wild-type、Keap1-KD、Keap1-HKO),继而使炎症因子、内质网应激基因、细胞凋亡基因、细胞坏死基因的表达(m KC、MIP-2、IL-1β、TNFα、Gadd45、Chop10、Bax、Caspase 3、Mcl、Noxa)按Nrf2-null、Wild-type、Keap1-KD、Keap1-HKO顺序逐渐下降。结论 Nrf2因子激活保护CCl4所致小鼠肝损伤反应。 | 陆远富 Connie Wu 刘杰 Curtis Dean Klaassen | 2015 | 遵义医学院学报2015，38，1： | 11 |
| 5 | Novel C-17 spirost protostane-type triterpenoids from Alisma plantago-aquatica with antiinflammatory activity in Caco-2 cells显示文摘Twenty-one protostane-type triterpenoids with diverse structures, including nine new compounds(1 e9), were isolated from the of Alisma plantago-aquatica Linn. Structurally, alisolides A-F(1 e6), composed of an oxole group coupled to a five-membered ring, represent unusual C-17 spirost protostane-type triterpenoids. Alisolide H(8) is a novel triterpenoid with an unreported endoperoxide bridge. Alisolide I(9) represents the first example of 23,24-acetal triterpenoid. Their structures were elucidated based on spectroscopic analysis, wherein the absolute configurations of 4-6, 8 were further confirmed by the Mo2(OAc)4-induced ECD method. Furthermore, all isolates were evaluated for their inhibitory effects on LPS-induced NO production in Caco-2 cells, and all the compounds showed remarkable inhibitory activities, with IC50 values in the range of 0.76 e38.20 mmol/L. | Qinghao Jin Jianqing Zhang Jinjun Hou Min Lei Chen Liu Xia Wang Yong Huang Shuai Yao Bang Yeon Hwang Wanying Wu Dean Guo | 2019 | Acta Pharmaceutica Sinica B2019，9，4： | 3 |
| 6 | Uniformly dispersed FeOx atomic clusters by pulsed arc plasma deposition: An efficient electrocatalyst for improving the performance of Li-O2 battery显示文摘现在的学习探索了一个新方法改进非宝贵的金属的催化活动,特别在电气化学的反应。弧分泌物生产的高度电离的 Fe 血浆一致地在多孔的碳底层上被扔并且在碳表面上形成了原子簇。同样准备的 FeO x /C 材料在不同的当前的率下面在一节可充电的 Li-O 2 电池作为阴极材料被测试。结果以周期生活和反应率在电池性能显示出重要改进。而且, X 光检查衍射(XRD ) 和扫描电子显微镜学(SEM ) 结果证明同样准备的阴极材料稳定了阴极并且减少了方面反应并且当前的率是在分泌物产品的成核的一个关键因素。 | Xiangyi Luo Jun Lu Evan Sohm Lu Ma Tianpin Wu Jianguo Wen Dantong Qiu YunKai Xu Yang Ren Dean J. Miller Khalil Amine | 2016 | Nano Research2016，9，7： | 3 |
| 7 | Demystifying graph processing frameworks and benchmarks显示文摘Dear editor,Graph algorithms have become important because of the increasing need to extract information from big data[1].Due to huge volumes of data and irregular communication patterns[2],it is a challenging task to apply graph algorithms efficiently. | Junyong DENG Qinzhe WU Xiaoyan WU Shuang SONG Joseph DEAN Lizy Kurian JOHN | 2020 | Science China(Information Sciences)2020，63，12： | 3 |
| 8 | Recent advances in alkaline hydrogen oxidation reaction显示文摘The development of highly efficient electrocatalysts toward hydrogen oxidation reaction(HOR)under alkaline media is essential for the commercialization of alkaline exchange membrane fuel cells(AEMFCs).However,the HOR kinetics in alkaline is two to three orders of magnitude slower than that in acid.More critically,fundamental understanding of the sluggish kinetics derived from the p H effect is still debatable.In this review,the recent development of understanding HOR mechanism and rational design of advanced HOR electrocatalysts are summarized.First,recent advances in the theories focusing on fundamental understandings of HOR under alkaline electrolyte are comprehensively discussed.Then,from the aspect of intermediates binding energy,optimizing hydrogen binding energy(HBE)and increasing hydroxyl binding energy(OHBE),the strategies for designing efficient alkaline HOR catalysts are summarized.At last,perspectives for the future research on alkaline HOR are pointed out. | Lixin Su Dan Gong Yiming Jin Dean Wu Wei Luo | 2022 | Journal of Energy Chemistry2022，31，3： | 2 |
| 9 | RAGE(yin versus )LRP (yang) balance regulates Alzheimer amliod --peptide clearance through transport across the blood - brain barrier显示文摘 | Deane R Wu Z Zlokvic BV | 2004 | Stoke2004，35，111： | 1 |
| 10 | Upregulated FoxM1 expression induced by hepatitis B virus X protein promotes tumor metastasis and indicates poor prognosis in hepatitis B virus-related hepatocellular carcinoma显示文摘 | Limin Xia Wenjie Huang Dean Tian Hongwu Zhu Yongguo Zhang Hao Hu Daiming Fan Yongzhan Nie Kaichun Wu | 2012 | Journal of Hepatology2012，，3： | 1 |
| 11 | Caspase activation and neuroprotection in caspase-3-deficient mice after in vivo cerebral ischemia and in vitro oxygen glucose deprivation显示文摘 | Le Dean A Wu Yongqin Huang Zhihong | 2002 | Proc Natl Acad Sci USA2002，99，15： | 1 |
| 12 | LRP/amyloid beta-peptide interaction mediates differential brain efflux of Aβ isoforms显示文摘 | Deane R Wu Z Sagare A | 2004 | Neuron2004，43，： | 1 |
| 13 | Flowering time control: another window to the connection between antisense RNA and chromatin显示文摘 | Robert Ietswaart Zhe Wu Caroline Dean | 2012 | Trends in Genetics2012，，9： | 1 |
| 14 | LRP/Amyloid-- Peptide Interaction Mediates Differential Brain Efflux of A-Isoforms显示文摘 | Deane R Wu ZH Sgare A | 2004 | Neuron2004，43，3： | 1 |
| 15 | Preparation and characterization of melt -extruded thermoplastic starch/clay nanocomposites 显示文摘 | Dean K Yu L Wu D | 2007 | Composites Science and Technology2007，67，3： | 1 |
| 16 | Role of angiotensin receptor subtypes in mescntcric vascular proliferation and Hypertrophy 显示文摘 | Cai Z Dean R Wu L | 1999 | hypertension1999，34，3： | 1 |
| 17 | RAGE(Yin)versus LRP(Yang)balance regulates Alzheimer’s amyloidβ-peptide clearance through transport across the blood-brain barrier显示文摘 | Deane R Wu Z Zlokovic BV | 2004 | Stroke2004，35，26： | 1 |
| 18 | RAGE (Yin) Versus LRP (Yang) Balance Regulates Alzheimer Arnyloid-Peptide Clearance ThroughTransport Across the Blood Brain Barrier 显示文摘 | Deane R Wu ZH Zlokovic BV | 2004 | Stroke2004，35，： | 1 |
| 19 | RAGE (yin) versus LRP (yang) balance regulates Alzheimer amyloid β-peptide clearance through transport across the blood-brain barrier 显示文摘 | Deane R Wu Z H Zlokovic B V | 2004 | Stroke2004，35，111： | 1 |
| 20 | Flowering time control:another window to the connection between antisense RNA and chromatin显示文摘 | IETSWAART R WU Z DEAN C | 2012 | Trends in Genetics2012，28，9： | 1 |
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