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| 1 | Fluid therapy for severe acute pancreatitis in acute response stage显示文摘为严重尖锐胰腺炎(树液) 的背景液体治疗不应该仅仅解决血体积的缺乏,而且在尖锐反应舞台阻止液体隐遁。最新,在那里,为液体治疗的策略没奉献给树液。那么,这研究被瞄准在树液的预后上调查液体治疗治疗的效果。76 个病人在 72 小时树液发作以内根据标准有希望地被招收的方法。他们随机被分到一个快速的液体扩大组(组我, n=36 ) 并且一个控制液体扩大组(组, n=40 ) 。血液动力学的混乱快速是任何一个(液体注入率是 10-15 ml 吗?? | MAO En-qiang TANG Yao-qing FEI Jian QIN Shuai WU Jun LI Lei MIN Dong ZHANG Sheng-dao | 2009 | Chinese Medical Journal2009，，2： | 89 |
| 2 | Artificial intelligence in healthcare:past,present and future显示文摘Artificial intelligence(AI)aims to mimic human cognitive functions.It is bringing a paradigm shift to healthcare,powered by increasing availability of healthcare data and rapid progress of analytics techniques.We survey the current status of AI applications in healthcare and discuss its future.AI can be applied to various types of healthcare data(structured and unstructured).Popular AI techniques include machine learning methods for structured data,such as the classical support vector machine and neural network,and the modern deep learning,as well as natural language processing for unstructured data.Major disease areas that use AI tools include cancer,neurology and cardiology.We then review in more details the AI applications in stroke,in the three major areas of early detection and diagnosis,treatment,as well as outcome prediction and prognosis evaluation.We conclude with discussion about pioneer AI systems,such as IBM Watson,and hurdles for real-life deployment of AI. | Fei Jiang Yong Jiang Hui Zhi Yi Dong Hao Li Sufeng Ma Yilong Wang Qiang Dong Haipeng Shen Yongjun Wang | 2017 | Stroke & Vascular Neurology2017，2，4： | 76 |
| 3 | Relationship between cytokines gene polymorphism and susceptibility to hepatitis B virus intrauterine infection显示文摘Background The influences of genomic background are confirmed in more diseases. Immunologic tolerance after intrauterine infection of hepatitis B virus is considered to occur in T cells. Cytokines work effectively in eliminating virus by immune system after hepatitis B virus infection. To explore the relationship between cytokines (tumor necrosis factor-α, interferon-γ, interleukin-4 and interleukin-10), which expressed abnormal quantity in the peripheral blood to intrauterine hepatitis B virus infectious children, gene single nucleotide polymorphism (SNP) and susceptibility to hepatitis B virus intrauterine infection. Methods This is a cross sectional study of molecular clinical epidemiology. The subjects in this study were selected from outpatients of hepatitis B vaccine follow-up special clinics of our hospital in the period. According to intrant criteria, the high risk children of hepatitis B virus (HBV) intrauterine infection were divided into immune failure group (group Ⅰ); and immune effective group (group Ⅱ) and non high risk children belonged to the control group. Four gene SNP sites of TNF-α -238, IFN-γ +874, IL-4 -590 and IL-10 -1082 were determined by real-time quantitative fluorescent polymerase chain reaction (PCR). Results The significant differences of TNF-α -238 A allele frequency were found between group Ⅰ and groupⅡ (χ2=6.797,P<0.05) and between groupⅠ and the control group (χ2=9.513,P<0.05). No evident differences of TNF-α -238 A were found between group Ⅱ and control group (χ2=0.047,P>0.05); the significant differences of IFN-γ +874 A allele frequency were found between groupⅠ and groupⅡ(χ2=7.238,P<0.05), and between groupⅠ and the control group (χ2=5.199,P<0.05). No evident differences were found between groupⅡ and the control group (χ2=0.602,P>0.05); the significant differences of IL-4 -590 C/T allele frequency were not found between groupⅠand group Ⅱ(χ2=0.632,P>0.05), also groupⅠ and the control group (χ2=0.584,P>0.05), and the groupⅡ and the control group (χ2=0.004,P>0.05)respectively; The significant differences of IL-10 -1082 G allele frequency were found between groupⅡ and groupⅠ (χ2=10.359,P<0.001), and between groupⅡ and the controls (χ2=35.418,P<0.001), but the significant differences were not found between groupⅠand the control group (χ2=1.759,P>0.05). Conclusions This study suggested the possibility that the TNF-α -238 A allele and IFN-γ +874 A allele were associated with HBV intrauterine infection. There was no evident relationship between IL-4 -590 C/T allele SNP and susceptibility to HBV intrauterine infection, but the IL-10 -1082 G allele was associated with preventive efficacy to HBV intrauterine infection. | ZHU Qi-rong GE Yan-ling GU Shao-qing YU Hui WANG Jian-she GU Xin-huan FEI Lin-e DONG Zuo-quan | 2005 | Chinese Medical Journal2005，，19： | 21 |
| 4 | Fast magnetic reconstruction of the portal vein with allogeneic blood vessels in canines显示文摘BACKGROUND:The resection and reconstruction of large vessels,including the portal vein,are frequently needed in tumor resection.Warm ischemia before reconstruction might have deleterious effects on the function of some vital organs and therefore,how to reconstruct the vessels quickly after resection is extremely important.The present study was to introduce a new type of magnetic compression anastomosis(MCA)device to establish a quick non-suture anastomosis of the portal vein after resection in canines.METHODS:The new MCA device consists of a pair of titanium alloy and neodymium-ferrum-boron magnet(Ti-Nd Fe B)composite rings.The Nd Fe B magnetic ring as a core of the device was hermetically sealed inside the biomedical titanium alloy case.Twelve canines were divided into two groups:a MCA group in which the end-to-end anastomoses was made with a new device after resection in the portal vein and a traditional manual suture(TMS)group consisted of 6 canines.The anastomosis time,anastomotic patency and quality were investigated at week 24 postoperatively.RESULTS:The portal vein was reconstructed successfully in all of the animals and they all survived.The duration of portal vein anastomosis was significantly shorter in the MCA group than in the TMS group(8.16±1.25 vs 36.24±2.17 min,P<0.05).Portography and ultrasound showed that the blood flow was normal without angiostenosis or thrombosis in all of the canines.Hematoxylin-eosin staining and electron microscope scanning showed in contrast to the TMS group,MCA anastomotic intimal was much smoother with more regularly arranged endothelial cells at week 24 postoperatively. | Shan-Pei Wang Xiao-Peng Yan Fei Xue Ding-Hui Dong Xu-Feng Zhang Feng Ma Hao-Hua Wang Yi Lv | 2015 | Hepatobiliary & Pancreatic Diseases International2015，14，3： | 20 |
| 5 | APPLICATION OF WHOLE BODY DIFFUSION WEIGHTED MR IMAGING FOR DIAGNOSIS AND STAGING OF MALIGNANT LYMPHOMA显示文摘Objective To evaluate the clinical impact of whole body diffusion weighted imaging (WB-DWI) on diagnosis and staging of malignant lymphoma. Methods Thirty-one patients with suspected lymphadenopathy were enrolled. WB-DWI was performed by using short TI inversion recovery echo-planar imaging sequence with free breathing and built-in body coil. Axial T2- weighted imaging images of the same location were used as reference. The results of WB-DWI were compared with pathological results and other imaging modalities. The mean apparent diffusion coefficient (ADC) values of different kinds of lymph nodes were compared. Results WB-DWI was positive in all 18 cases with lymphoma, 5 cases with metastatic lymph nodes and 4 of 8 cases with benign lymphadenopathy. The mean ADC value of lymphomatous, metastatic and benign lymph nodes was (0.87 ± 0.17) × 10-3, (0.98 ± 0.09) × 10-3 and (1.20 ± 0.10) × 10-3 mm2/s. There was significant difference in ADC value between benign lymph nodes and other two groups (P < 0.01). The sensitivity, specificity and accuracy of WB-DWI in diagnosis of lymphoma were 100% (18/18), 30.8% (4/13) and 71.0% (22/31). When an ADC value of 1.08 × 10-3 mm2/s was used as the threshold value for differentiating malignant from benign lymph nodes, the best results were obtained with sensitivity of 87.8% and specificity of 91.3%. Sixteen of eighteen cases (88.9%) of lymphoma were accurately staged in accordance with clinical staging. Conclusions WB-DWI is a sensitive, but less specific technique for diagnosis of lymphoma. It is difficult to differentiate lymphomatous from metastatic lymph nodes using WB-DWI. However, it is a valuable imaging modality for staging of patients with malignant lymphoma. | Shuo Li Hua-dan Xue Jian Li Fei Sun Bo Jiang Dong Liu Hong-yi Sun Zheng-yu Jin | 2008 | Chinese Medical Sciences Journal2008，23，3： | 18 |
| 6 | Clinical efficacy of 0.1% pranoprofen in treatment of dry eye patients:a multicenter, randomized, controlled clinical trial显示文摘 | Chen Jingyao Dong Fei Chen Wei Sun Xuguang Deng Yingping Hong Jing Zhang Mingchang Yang Wenzhao Liu Zuguo Xie Lixin | 2014 | Chinese Medical Journal2014，，13： | 18 |
| 7 | Virulence and potential pathogenicity of coccoid Helicobacter pylori induced by antibiotics显示文摘AIM To explore the virulence and the potential pathogenicity of coccoid Helicobacter pylori (H. pylori) transformed from spiral form by exposure to antibiotic.METHODS Three strains of H. pylori, isolated from gastric biopsy specimens of confirmed peptic ulcer, were converted from spiral into coccoid from by exposure to metronidazole.Both spiral and coccoid form of H. pylori were tested for the urease activity, the adherence to Hep-2 cells and the vacuolating cytotoxicity to Hela cells, and the differences of the protein were analysed by SDS-PAGE and Western blot,The mutation of the genes including ureA, ureB,hpaA; vacA and cagA, related with virulence,was detected by means of PCR and PCR-SSCP.RESULTS In the coccoid H. pylori, the urease activity, the adherence to Hep-2 cells and the vacuolating cytotoxicity to Hela cells alldecreased. In strain F44, the rate and index of adherence reduced from 70.0% ± 5.3% to 33% ±5.1% and from 2.6 ±0.4 to 0.96 ±0.3 (P<0.01),respectively. The invasion of coccoid H. pylori into Hep-2 cell could be seen under electronmicroscope. SDS-PAGE showed that the content of the protein with the molecular weight over Mr74 000 decreased, and the hybriditional signal in band Mr 125 000 weakened, while the band Mr 110000 and Mr63000 strengthened in coccoid H. pylori as shown in Western blot. The results of PCR were all positive, and PCR-SSCP indicated that there may exist the point mutation in gene hpaA or vacA.CONCLUSION The virulence and the proteins with molecular weight over Mr74 000 in coccoid H. pylori decrease, but no deletion exists in amplification fragments from ureA, ureB, hpaA,vacA and cagA genes, suggesting that coccoid H. pylori may have potential pathogenicity. | Fei Fei She1 Dong Hui Su1 Jian Yin Lin2 Lin Ying Zhou3 1Department of Microbiology, Fujian Medical University. Fuzhou 350004, Fujian Province, China2Department of Molecular Medicine, Fujian Medical University, Fuzhou 350004, Fujian Province, China 3Laboratory of Electron Microscope, Fujian Medical University, Fuzhou 350004. Fujian Province. ChinaFei Fei She. graduated from Fujian Medical University as a postgraduate in 1991, now associate professor of microbiology and immunology, specialized in molecular biology of pathogen, having 15 papers published. | 2001 | World Journal of Gastroenterology2001，7，2： | 18 |
| 8 | Three-dimensional Evolutionary Models of the Qiongxi Structures,Southwestern Sichuan Basin,China:Evidence from Seismic Interpretation and Geomorphology显示文摘褶层结束是在有压缩性的差错相关的褶层的学习的特色。如此的结束能由于戳差错上的排水量的损失或 / 并且形成一个侧面或倾斜的斜面。因此,高质量的地震数据将明白地帮助定义哪个机制应该为给定的差错相关的褶层的结束负责。在四川盆的 Qiongxi 和 Qiongxinan 结构,中国是拥有在他们之间的北结束和结构的僵绳的自然差错繁殖褶层的例子。合拢 / 指责几何学和沿着罢工排水量变化被工业 3-D 抑制地震体积。我们解释北结束和结构的僵绳附近的褶层的 plunge 由于排水量的损失罢工。与北结束联系的差错几何学变化从一在 Qiongxinan 结构的冠扁平斜面在排水量最大的地方,到简单地 Qiongxi 结构的北尖端附近的一个斜面,没有形成一个侧面或倾斜的斜面。在这研究,我们也使用排水模式,在沿着一个差错的排水量与推进为 Qiongxinan 的侧面的繁殖建议一个模型的持续时间成正比的 Qiongxinan 结构和假设的冠保存的胚胎的结构和 Qiongxi 结构。明确地,我们建议结构首先在易碎的单位以内作为一个孤立的差错斜面开始了。与弄短增加,差错变得以更弱的页岩单位与更低的分队连接创造 hybridized 差错繁殖褶层。我们的模型为 Qiongxinan 和 Qiongxi 结构的侧面的繁殖历史建议可能的解释,并且也提供一条其他的途径给在迟了的新生代在西南的四川盆证实以前的 Pingluoba 结构的活动。 | JIA Qiupeng JIA Dong LUO Liang CHEN Zhuxin LI Yiquan DENG Fei SUN Shengsi LI Haibin | 2009 | Acta Geologica Sinica(English Edition)2009，83，2： | 17 |
| 9 | Incubation and application of transgenic green fluorescent nude mice in visualization studies on glioma tissue remodeling显示文摘 | DONG Jun, DAI Xing-liang LU Zhao-hui FEI Xi-feng CHEN Hua ZHANG Quan-bin ZHAO Yao-dong WANG Zhi-min WANG Ai-dong LAN Qing HUANG Qiang | 2012 | Chinese Medical Journal2012，，24： | 17 |
| 10 | Effects of Honghua preserved amniotic membrane on scar healing in experimental glaucoma surgery显示文摘AIM:To investigate the efficacy and safety of Honghua preserved amniotic membrane(AM)for preventing scar formation of the filtering bleb in a rabbit model of glaucoma trabeculectomy surgery.METHODS:Totally 36 rabbits(36 eyes)were randomly divided into 3 groups:the experimental group(ocular trabeculectomy in combination with Honghua preserved AM transplantation),the control group(ocular trabeculectomy surgery in combination with AM implantation),and the blank group(single trabeculectomy).Clinical observations[including intraocular pressure(IOP),filtering blebs and complications],MassonTrichrome staining,real-time quantitative reverse transcription-polymerase chain reaction(real-time PCR),Western blot were performed on different time points(D1,D7,D14,D21 and D56)after the surgery.RESULTS:After operated for 14d,there were statistically significant differences in the filtering blebs compared to the situation before operation(P<0.05),whereas no statistically difference on that among three groups(P>0.05).After 21d,the IOP of experimental group was lowest(P<0.05).There was significant difference between control group and blank group(P<0.05).On postoperative D14,the mean number of fibroblasts in the experimental group was significantlylower(40.6±10.2)compared to those in the control group(54.4±10.8)and blank group(68.2±11.6)(P<0.05,respectively).The mean numbers of the macrophage in the experimental and control groups were respcitively significantly lower versus the blank group(P<0.05,P<0.05,respectively).Compared to that in blank group,the level of transforming growth factor-β(TGF-β1)expression in sclera and conjunctival areas was reduced in the experimental and control groups on protein and mRNA level(P<0.05),but not significant difference between these two groups(P>0.05).CONCLUSION:The trabeculectory surgery with Honghua preserved AM can control IOP,sustain the functional filtration bleb,inhibit the proliferation of fibroblasts and open the filtrating pathway on the rabbit glaucoma models. | Yi Shao Yao Yu Qiu-Ping Liu Jing-Ming Li Fei Dong Xin Huang Chong-Gang Pei Ping Tu Hen-Hui Li Gui-Ping Gao | 2014 | International Journal of Ophthalmology(English edition)2014，7，2： | 16 |
| 11 | MR DIFFUSION WEIGHTED IMAGING FOR EVALUATION OF RADIOTHERAPEUTIC EFFECTS ON RABBIT VX2 TUMOR MODEL显示文摘Objective To investigate the feasibility of magnetic resonance (MR) diffusion weighted imaging (DWI) for evaluation of radiotherapeutic effects on rabbit VX2 tumor model. Methods Sixteen New Zealand white rabbits received a subcutaneous implantation of VX2 tumor cell suspension 0.5 mL (4×107 cells/mL) in their right thighs to set up tumor model. And 2 weeks later they were randomly divided into therapy group (Group T, n = 10) and control group (Group C, n = 6). Group T received radiotherapy at a single dose of 10 Gy. MR imaging (MRI) scan including short TI inversion recovery echo-planar imaging DWI, T1-weighted imaging (T1WI) and T2-weighted imaging (T2WI) sequences were performed 1 day prior to as well as 1 day, 2 days, 3 days and 7 days after radiotherapy. Group C received only MRI scan at the same time points without any treatment. MRI appearance on T2WI, T1WI, and DWI images was compared and tumor volume was calculated. Apparent diffusion coefficient (ADC) values of the tumor were evaluated in all cases. HE staining was used for pathological study. Results Necrosis (n = 8) and hemorrhage (n = 2) were seen gradually on T2WI and T1WI images of Group T after time point of day 2 after irradiation. In Group C, no obvious necrosis was found until day 7. There was no significant difference in tumor volume between the two groups before radiotherapy. After radiotherapy, tumors in Group T showed a gradual growth but not as obvious as Group C. There was a significant difference in tumor volume between the two groups from day 2 on (P < 0.05). ADC value changed dramatically right from the 1st day after radio- therapy in Group T [(0.99 ± 0.15) ×10-3 mm2/s for 1 day before radiotherapy, (1.23 ± 0.08) ×10-3, (1.45 ± 0.07) ×10-3, (1.63 ± 0.06) ×10-3, and (2.02 ± 0.18) ×10-3 mm2/s for day 1, 2, 3, and 7]; and ADC value had no significant changes after radiotherapy in Group C except day 7 [(1.07 ± 0.08) ×10-3 mm2/s for 1 day before radiotherapy, (1.03 ± 0.04) × 10-3, (1.05 ± 0.02) ×10-3, (1.05 ± 0.05) ×10-3, and (0.95 ± 0.07) ×10-3 mm2/s for day 1, 2, 3, and 7]. There was significant difference in ADC value between the two groups for each time point after radiotherapy (P < 0.01). Pathological study showed that the number of viable tumor cells in Group T decreased 1 day after radiotherapy, and the inflammatory cell infiltration was marked and almost all viable tumor cells disappeared by day 7 after radiotherapy. Conclusions DWI is a new promising technique for monitoring radiotherapy outcomes. ADC value may give a prior clue on physiological changes of radiotherapy before routine MRI could tell. | Shuo Li Hua-dan Xue Xin-hai Wang Fei Sun Bo Jiang Dong Liu Jing Lei Zheng-yu Jin | 2008 | Chinese Medical Sciences Journal2008，23，3： | 15 |
| 12 | Low Root Zone Temperature Limits Nutrient Effects on Cucumber Seedling Growth and Induces Adversity Physiological Response显示文摘Effects of root-zone temperatures (RZT) (12°C-RZT and 20°C-RZT) and different N, P, and K nutrient regimes on the growth, reactive oxygen species (ROS), and antioxidant enzyme in cucumber seedlings were investigated in hydroponics. Strong interactions were observed between RZT and nutrient on the dry weight (P=0.001), root length (P=0.001) and leaf area (P=0.05). Plant dry weights were suppressed at low RZT of 12°C, while higher biomass and growth of cucumber seedlings were produced at elevated RZT of 20°C under each nutrient treatment. Growth indexes (plant height, internode length, root length, and leaf area) at 12°C-RZT had less difference among nutrient treatments, but greater response was obtained for different nutrients at high RZT. RZT had larger effects (P=0.001) on cucumber seedling growth than nutrients. In addition, N was more effective nutrients to plant growth than P and K under low root temperature to plant growth. Higher hydrogen peroxide (H 2 O 2 ), malondialdehyde (MDA), soluble sugar (SS) contents in leaves were observed at 12°C-RZT in all nutrient treatments than those at 20°C-RZT, indicating the chilling adversity damaged to plant growth. In general, antioxidant enzyme had larger response under low root-zone temperature. Superoxide dismutase (SOD) activities were higher in both leaves and roots while peroxidase (POD) and catalase (CAT) showed large different action in leaves and roots at both the two root-zone temperature. | YAN Qiu-yan DUAN Zeng-qiang MAO Jing-dong LI Xun DONG Fei | 2013 | Journal of Integrative Agriculture2013，12，8： | 15 |
| 13 | Acupuncture Regulates the Balance of CD4^+ T Cell Subtypes in Experimental Asthma Mice显示文摘Objective: To evaluate the involvement of different CD4+ T cell subtypes in the anti-asthmatic effects of acupuncture in asthmatic mice. Methods: BALB/c mice were challenged by ovalbumin (OVA) for the establishment of experimental asthma model. Mice were divided into 4 groups by a random number table including the normal control, asthma model, acupuncture and sham acupuncture groups (14 per group). Acupoints Dazhui (GV 14), bilateral Fengmen (BL 12) and Feishu (BL 13) were selected for manual acupuncture treatment every other day for 4 weeks and Huantiao (GB 30) was selected for sham acupuncture. Airway hyperresponsiveness was examined by Buxco Pulmonary System. Pulmonary histopathology analysis was performed for inflammatory cell infiltration and mucus hypersecretion by haematoxylin eosin staining and periodic acid-Schiff staining. Inflammatory mediators assays of serum were investigated by enzyme-linked immunosorbent assay and Bio-Plex. CD4+ T cell subpopulations including the expression levels of important factors in T lymphocyte polarization in lung tissue were examined by flow cytometric and Western blot analyses. Related pathways were detected by Western blot assay. Results: Compared with the OVA-induced asthma model group, acupuncture could attenuate airway hyperresponsiveness, inhibit inflammatory cell infiltration and mucus hypersecretion (P<0.05 or P<0.01). Furthermore, acupuncture increased the expressions of T-bet and Foxp3+, the cell numbers of CD4+ interferon gamma (IFN-γ)+ and CD4+ Foxp3+ in lung tissue and the level of Treg type cytokine interleukin (IL)-10 in serum (P<0.05 or P<0.01). Meanwhile, acupuncture reduced the RAR-related orphan receptor gamma t (RORγ t) level, the cell numbers of CD4+ IL-17A+ as well as the levels of IL-5, IL-13 and IL-17A in serum (P<0.05 or P<0.01). In addition, both acupuncture and sham acupuncture could inhibit the phosphorylation of p38 and p44/42 (P<0.01). Conclusion: Acupuncture could alleviate allergic airway inflammation by strengthening the activities of Th1 and Treg, thus regulating the balance of CD4+ T cell subtypes in experimental asthmatic mice. | DONG Ming WANG Wen-qian CHEN Juan LI Mi-hui XU Fei CUI Jie DONG Jing-cheng WEI Ying | 2019 | Chinese Journal of Integrative Medicine2019，25，8： | 15 |
| 14 | Laparoscopic cystogastrostomy for the treatment of pancreatic pseudocysts: A case report显示文摘Pancreatic pseudocysts(PPs) are collections o pancreatic secretions that are lined by fibrous tissue and may contain necrotic debris or blood. The interventions including percutaneous, endoscopic o surgical approaches are based on the size, location symptoms and complications of a pseudocyst With the availability of advanced imaging system and cameras, better hemostatic equipments and excellent laparoscopic techniques, most pseudocyst can be found and managed by laparoscopy. We describe a case of a 30-year-old male patient with a pancreatic pseudocyst amenable to laparoscopi cystogastrostomy. An incision was made through the anterior gastric wall to expose the posterior gastri wall in close contact with the pseudocyst using an ultrasonically activated scalpel. Then, another incision was made for cystogastrostomy to obtain complete and unobstructed drainage. The patient recovered well after operation and was symptom-free during a 6-mo follow-up, suggesting that laparoscopi cystogastrostomy is a safe and effective alternative to open cystogastrostomy for minimally invasive management of PPs. | Qin-song Sheng Da-Zhi Chen Ren Lang Zhong-Kui Jin Dong-Dong Han Li-Xin Li Yong-Jiu Yang Ping Li Fei Pan Dong Zhang Zhao-Wei Qu Qiang He | 2008 | World Journal of Gastroenterology2008，14，30： | 15 |
| 15 | MiR-139-5p inhibits migration and invasion of colorectal cancer by downregulating AMFR and NOTCH1显示文摘MicroRNAs (miRNAs ) 由抑制最近在 carcinogenesis 和转移在我们 non-protein-coding RNA 的角色的理解带给卓见的 posttranscriptional 施加功能。在这研究,我们在 CRC 在 colorectal 癌症(CRC ) 和它的潜在的临床的应用程序描述了函数和 miR-139-5p 的分子的机制。我们发现 miR-139-5p 是显著地, CRC 与邻近的 noncancerous 纸巾(NCT ) 相比取样的在 73.8% 的 downregulated,和减少的 miR-139-5p 与穷人被联系预后。功能的分析证明 miR-139-5p 的那个宫外的表达式在 vivo 在 vitro 和转移压制了 CRC 房间移植和侵略。机械学的调查表明 miR-139-5p 由指向 AMFR 和 NOTCH1 压制 CRC 房间侵略和转移。二基因 phenocopied 击倒 CRC 上的 miR-139-5p 的禁止的效果转移。而且,二基因的蛋白质层次与 NCT 相比是在 CRC 样品的 upregulated,并且相反地与 miR-139-5p 表示相关。增加的 NOTCH1 蛋白质表示与 CRC 病人的差的预后被相关。一起,我们的数据显示 miR-139-5p 为 CRC 是潜在的肿瘤 suppressor 和预示的因素,并且指向 miR-139-5p 可以镇压 CRC 的转移并且改善幸存。 | Mingxu Song Yuan Yin Jiwei Zhang Binbin Zhang Zehua Bian Chao Quan Leyuan Zhou Yaling Hu Qifeng Wang Shujuan Ni Bojian Fei Weili Wang Xiang Du Dong Hua Zhaohui Huang | 2014 | Protein & Cell2014，5，11： | 14 |
| 16 | Genotypic Characterization of Methicillin-resistant Staphylococcus aureus Isolated from Pigs and Retail Foods in China显示文摘Objective To investigate the genotypic diversity of Methicillin-resistant Staphylococcus aureus(MRSA) isolated from pigs and retail foods from different geographical areas in China and further to study the routes and rates of transmission of this pathogen from animals to food. Methods Seventy-one MRSA isolates were obtained from pigs and retail foods and then characterized by multi-locus sequencing typing(MLST), spa typing, multiple-locus variable number of tandem repeat analysis(MLVA), pulsed-field gel electrophoresis(PFGE), and antimicrobial susceptibility testing. Results All isolated MRSA exhibited multi-drug resistance(MDR). Greater diversity was found in food-associated MRSA(7 STs, 8 spa types, and 10 MLVA patterns) compared to pig-associated MRSA(3 STs, 1 spa type, and 6 MLVA patterns). PFGE patterns were more diverse for pig-associated MRSA than those of food-associated isolates(40 vs. 11 pulse types). Among the pig-associated isolates, CC9-ST9-t899-MC2236 was the most prevalent clone(96.4%), and CC9-ST9-t437-MC621(20.0%) was the predominant clone among the food-associated isolates. The CC9-ST9 isolates showed significantly higher antimicrobial resistance than other clones. Interestingly, CC398-ST398-t034 clone was identified from both pig-and food-associated isolates. Of note, some community-and hospital-associated MRSA strains(t030, t172, t1244, and t4549) were also identified as food-associated isolates. Conclusion CC9-ST9-t899-MC2236-MDR was the most predominant clone in pigs, but significant genetic diversity was observed in food-associated MRSA. Our results demonstrate the great need for improved surveillance of MRSA in livestock and food and effective prevention strategies to limit MDR-MRSA infections in China. | WANG Wei LIU Feng ZULQARNAIN Baloch ZHANG Cun Shan MA Ke PENG Zi Xin YAN Shao Fei HU Yu Jie GAN Xin DONG Yin Ping BAI Yao LI Feng Qin YAN Xiao Mei MA Ai Guo XU Jin | 2017 | Biomedical and Environmental Sciences2017，30，8： | 13 |
| 17 | IL-10-producing regulatory B cells restrain the T follicular helper cell response in primary Sjögren’s syndrome显示文摘Increased numbers of T follicular helper(Tfh)cells have been implicated in the development of autoimmune diseases including primary Sjögren’s syndrome(pSS),but how the Tfh cell response is regulated during autoimmune pathogenesis remains largely unclear.Here,we first found negative correlations between IL-10^(+)regulatory B(Breg)cell numbers and Tfh cell responses and disease activity in patients with pSS and mice with experimental Sjögren’s syndrome(ESS).Moreover,we detected high expression of IL-10 receptor on Tfh cells and their precursors in both humans and mice.In culture,IL-10 suppressed human and murine Tfh cell differentiation by promoting STAT5 phosphorylation.By using an adoptive transfer approach and two-photon live imaging,we found significantly increased numbers of Tfh cells with enhanced T cell homing into B cell follicles in the draining cervical lymph nodes of RAG-2−/−mice transferred with IL-10-deficient B cells during ESS development compared with those of RAG-2−/−mice transferred with wild-type B cells.In ESS mice,CD19^(+)CD1d^(hi)CD5^(+)Breg cells with decreased IL-10 production exhibited severely impaired suppressive effects on T cell proliferation.Consistently,CD19^(+)CD24^(+)CD38^(hi) Breg cells from pSS patients showed significantly reduced IL-10 production with defective inhibitory function in the suppression of autologous Tfh cell expansion.Furthermore,the adoptive transfer of IL-10-producing Breg cells markedly suppressed the Tfh cell response and ameliorated ESS progression in ESS mice.Together,these findings demonstrate a critical role for IL-10-producing Breg cells in restraining the effector Tfh cell response during pSS development. | Xiang Lin Xiaohui Wang Fan Xiao Kongyang Ma Lixiong Liu Xiaoqi Wang Dong Xu Fei Wang Xiaofei Shi Dongzhou Liu Yan Zhao Liwei Lu | 2019 | Cellular & Molecular Immunology2019，16，12： | 13 |
| 18 | Parthenogenetic haploid embryonic stem cells produce fertile mice显示文摘 | Haifeng Wan Zhengquan He Mingzhu Dong Tiantian Gu Guan-Zheng Luo Fei Teng Baolong Xia Wei Li Chunjing Feng Xin Li Tianda Li Ling Shua Rui Fu Liu Wang Xiu-Jie Wang Xiao-Yang Zhao Qi Zhou | 2013 | Cell Research2013，23，11： | 13 |
| 19 | Toripalimab combined with lenvatinib and GEMOX is a promising regimen as first-line treatment for advanced intrahepatic cholangiocarcinoma:a single-center,single-arm,phase 2 study显示文摘Advanced intrahepatic cholangiocarcinoma(ICC)has a dismal prognosis.Here,we report the efficacy and safety of combining toripalimab,lenvatinib,and gemcitabine plus oxaliplatin(GEMOX)as first-line therapy for advanced ICC.Thirty patients with pathologically confirmed advanced ICC received intravenous gemcitabine(1 g/m2)on Days 1 and 8 and oxaliplatin(85 mg/m2)Q3W for six cycles along with intravenous toripalimab(240 mg)Q3W and oral lenvatinib(8 mg)once daily for one year.The expression of programmed death-ligand 1(PD-L1)and genetic status was investigated in paraffin-embedded tissues using immunohistochemistry and whole-exome sequencing(WES)analysis.The primary endpoint was the objective response rate(ORR).Secondary outcomes included safety,overall survival(OS),progression-free survival(PFS),disease control rate(DCR)and duration of response(DoR).As of July 1,2022,the median follow-up time was 23.5 months,and the ORR was 80%.Twenty-three patients achieved partial response,and one achieved complete response.Patients(21/30)with DNA damage response(DDR)-related gene mutations showed a higher ORR,while patients(14/30)with tumor area positivity≥1(PD-L1 staining)showed a trend of high ORR,but without significant difference.The median OS,PFS,and DoR were 22.5,10.2,and 11.0 months,respectively.The DCR was 93.3%.Further,56.7%of patients experienced manageable grade≥3 adverse events(AEs),commonly neutropenia(40.0%)and leukocytopenia(23.3%).In conclusion,toripalimab plus lenvatinib and GEMOX are promising first-line regimens for the treatment of advanced ICC.A phase-III,multicenter,double-blinded,randomized study to validate our findings was approved by the National Medical Products Administration(NMPA,No.2021LP01825). | Guo-Ming Shi Xiao-Yong Huang Dong Wu Hui-Chuan Sun Fei Liang Yuan Ji Yi Chen Guo-Huan Yang Jia-Cheng Lu Xian-Long Meng Xin-Ying Wang Lei Sun Ning-Ling Ge Xiao-Wu Huang Shuang-Jian Qiu Xin-Rong Yang Qiang Gao Yi-Feng He Yang Xu Jian Sun Zheng-Gang Ren Jia Fan Jian Zhou | 2023 | Signal Transduction and Targeted Therapy2023，8，4： | 12 |
| 20 | Targeting cancer stem cell pathways for cancer therapy显示文摘Since cancer stem cells(CSCs)were first identified in leukemia in 1994,they have been considered promising therapeutic targets for cancer therapy.These cells have self-renewal capacity and differentiation potential and contribute to multiple tumor malignancies,such as recurrence,metastasis,heterogeneity,multidrug resistance,and radiation resistance.The biological activities of CSCs are regulated by several pluripotent transcription factors,such as OCT4,Sox2,Nanog,KLF4,and MYC.In addition,many intracellular signaling pathways,such as Wnt,NF-κB(nuclear factor-κB),Notch,Hedgehog,JAK-STAT(Janus kinase/signal transducers and activators of transcription),PI3K/AKT/mTOR(phosphoinositide 3-kinase/AKT/mammalian target of rapamycin),TGF(transforming growth factor)/SMAD,and PPAR(peroxisome proliferator-activated receptor),as well as extracellular factors,such as vascular niches,hypoxia,tumor-associated macrophages,cancer-associated fibroblasts,cancer-associated mesenchymal stem cells,extracellular matrix,and exosomes,have been shown to be very important regulators of CSCs.Molecules,vaccines,antibodies,and CAR-T(chimeric antigen receptor T cell)cells have been developed to specifically target CSCs,and some of these factors are already undergoing clinical trials.This review summarizes the characterization and identification of CSCs,depicts major factors and pathways that regulate CSC development,and discusses potential targeted therapy for CSCs. | Liqun Yang Pengfei Shi Gaichao Zhao Jie Xu Wen Peng Jiayi Zhang Guanghui Zhang Xiaowen Wang Zhen Dong Fei Chen Hongjuan Cui | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 12 |
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