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| 1 | Tetracycline-inducible protein expression in pancreatic cancer cells: Effects of CapG overexpression显示文摘AIM: To establish stable tetracycline-inducible pancreatic cancer cell lines. METHODS: Suit-2, MiaPaca-2, and Panc-1 cells were transfected with a second generation reverse tetracycline-controlled transactivator protein (rtTA2S-M2), under the control of either a cytomegalovirus (CMV) or a chicken β-actin promoter, and the resulting clones were characterised.RESULTS: Use of the chicken (β-actin) promoter proved superior for both the production and maintenance of doxycycline-inducible cell lines. The system proved versatile, enabling transient inducible expression of a variety of genes, including GST-P, CYP2E1, S100A6, and the actin capping protein, CapG. To determine the physiological utility of this system in pancreatic cancer cells, stable inducible CapG expressors were established. Overexpressed CapG was localised to the cytoplasm and the nuclear membrane, but was not observed in the nucleus. High CapG levels were associated with enhanced motility, but not with changes to the cell cycle, or cellular proliferation. In CapG-overexpressing cells, the levels and phosphorylation status of other actin-moduating proteins (Cofilin and Ezrin/Radixin) were not altered. However, preliminary analyses suggest that the levels of other cellular proteins, such as ornithine aminotransferase and enolase, are altered upon CapG induction. CONCLUSION: We have generated pancreatic-cancer derived cell lines in which gene expression is fully controllable. | Sarah Tonack Sabina Patel Mehdi Jalali Taoufik Nedjadi Rosalind E Jenkins Christopher Goldring John Neoptolemos Eithne Costello | 2011 | World Journal of Gastroenterology2011，17，15： | 4 |
| 2 | The role acid in the formation of hydrogen-bonded networks featuring 4,4'-dicarboxv-2,2'-bipyridine( H2dcbp) : Synthesis, structural and magnetic eharacterisation of I · H2O}and 显示文摘 | Eithne Tynan Paul Jensen Paul E Kruger | 2005 | Cryst Eng Comm2005，7，： | 1 |
| 3 | Coordination and hydrogen bonded networks featuring 4,4'-dicarboxy-2,2'- bipyridine( H2dcbp) : structural characterisation of H2debp, · 4H2 O, and { · 2 H2O}n 显示文摘 | Eithne Tynan Paul Jensen Paul E Kruger | 2003 | Dalton Trans2003，，： | 1 |
| 4 | The ligand, the metal and the 'Holey'-host: Synthesis, structural and magnetic characterization of Co( Ⅱ ) , Ni ( Ⅱ ) and Mn ( Ⅱ ) metal-organic frameworks incorporating 4,4'-dicarboxy-2,2'-bipyridine显示文摘 | Eithne Tynan Paul Jensen Paul E Kruger | 2004 | Dalton Trans2004，，： | 1 |
| 5 | Solvent templated synthesis of metal-organic frmeworks : structural charactersation and properties of the 3D network isomers t ·2H2 O}n显示文摘 | Eithne Tynan Paul Jensen Paul E Kruger | 2004 | Chem Commun2004，，： | 1 |
| 6 | Posttransplant lymphoproliferative disorder presenting as a nonhealing extraction socket: a case report and review of the literature显示文摘 | Holly Cole-Hawkins Eithne Fyfe Chris Price Miranda Pring | 2012 | Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology2012，，5： | 1 |
| 7 | The unfolded protein response regulator GRP78 is a novel predictive biomarker in colorectal cancer显示文摘 | Michael Thornton Mohammed A. Aslam Elizabeth M. Tweedle Chin Ang Fiona Campbell Richard Jackson Eithne Costello Paul S. Rooney Nikolina Vlatkovi? Mark T. Boyd | 2013 | Int J Cancer2013，，6： | 1 |
| 8 | Commonage land and farmer uptake of the rural environment protection scheme in Ireland显示文摘 | Tom M. Van Rensburg Eithne Murphy Paul Rocks | 2008 | Land Use Policy2008，，2： | 1 |
| 9 | Chief executive leadership style, consensus decision making, and top management team effectiveness显示文摘 | Patrick C. Flood Eithne Hannan Ken G. Smith Thomas Turner Michael A. West Jeremy Dawson | 2000 | European Journal of Work and Organizational Psychology2000，，3： | 1 |
| 10 | Circulating microRNAs as potential markers of human drug‐induced liver injury显示文摘 | Philip J. Starkey Lewis James Dear Vivien Platt Kenneth J. Simpson Darren G.N. Craig Daniel J. Antoine Neil S. French Neeraj Dhaun David J. Webb Eithne M. Costello John P. Neoptolemos Jonathan Moggs Chris E. Goldring B. Kevin Park | 2011 | Hepatology2011，，： | 1 |
| 11 | An Empirical Analysis of Short- run and Long- run Irish Export Functions: does exchange rate volatility matter显示文摘 | Don Bredin Stilianos Fountas Eithne Murphy | 2003 | International Review of Applied Economics2003，，2： | 1 |
| 12 | Commonage land and farmer uptake of the rural environment protection scheme in Ireland显示文摘 | Tom M. Van Rensburg Eithne Murphy Paul Rocks | 2008 | Land Use Policy2008，，2： | 1 |
| 13 | Assembly,Electrochemical Characterisation and Electrocatalytic Ability of Multilayer Films Based on2+,and the Dawson Heteropolyanion,6-显示文摘 | Eithne D Timothy M C | 2005 | J Electroanal Chem2005，574，： | 1 |
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