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| 1 | Pyrroloquinoline quinone regulates the redox status in vitro and in vivo of weaned pigs via the Nrf2/HO-1 pathway显示文摘Background:Oxidative stress is a main cause of piglet gut damage and diarrhea.Pyrroloquinoline quinone(PQQ),is a novel redox cofactor with antioxidant properties.However,the effect and mechanism that PQQ supplementation decreases oxidative injury in weaned pigs is not understood.Therefore,the aim of this study is to confirm the effect of PQQ on regulating redox status in weaned pigs and the mechanism for antioxidant function by porcine intestinal epithelial cell line(IPEC-J2)challenged with H_(2)O_(2).Results:Experiment 1,144 Duroc×Landrace×Yorkshire pigs(weaned at 28 d)were allocated to four groups:received a basal diet(control)and diets supplemented with 0.15%,0.30%and 0.45%PQQ,respectively.On d 28,growth performance,diarrhea incidence and redox factors were measured.Experiment 2,IPEC-J2 were treated with or without PQQ in the presence or absence of H_(2)O_(2)for indicated time points.Experiment 3,IPEC-J2 were transfected with or without Nrf2 siRNA,then treated according to Experiment 2.The cell viability,redox factors,protein of tight junctions and Nrf2 pathway were determined.In vivo,PQQ supplementation demonstrated dose-related improvements in average daily gain,and gain to feed ratio(Linear P<0.05).During d 0–28,compared to controls,0.45%PQQ supplementation for pigs decreased diarrhea incidence and MDA content in liver and jejunum,and increased concentration of SOD in liver;0.3%PQQ supplementation decreased ileal and liver MDA concentration;and 0.15%PQQ supplementation decreased ileal MDA concentration(P<0.05).In vitro,compared to cells cultured with H_(2)O_(2),pre-treatment with PQQ increased cell viability,tight junction proteins expression including ZO-1,ZO-2,Occludin and Claudin-1;and decreased ROS concentration and level of Caspase-3(P<0.05);as well as upregulated the ratio of Bcl-2 to Bax and protein expression of nuclear Nrf2,HO-1.Notably,Nrf2 knockdown by transfection with Nrf2 siRNA largely abrogated the positive effects of PQQ pretreatment on H_(2)O_(2)-induced intracellular changes.Conclusions:PQQ administration attenuated oxidative stress in weaned pigs which is associated with activation of Nrf2/HO-1 pathway. | Caiyun Huang Zijuan Fan Dandan Han Lee J.Johnston Xi Ma Fenglai Wang | 2021 | Journal of Animal Science and Biotechnology2021，12，4： | 4 |
| 2 | Nuclear proteome profile of C57BL/6J mouse liver显示文摘The liver proteome can serve as a reference to better understand both disease mechanisms and possible therapeutics,since the liver is an important organ in the body that performs a large number of tasks.Here we identify the organelle proteome of C57BL/6J mouse liver nuclei as a promising strategy to enrich low abundance proteins,in the sense that analysis of whole liver cells is rather complex for current techniques and may not be suitable for proteins with low abundance.Evaluation of nucleus integrity and purity was performed to demonstrate the effectiveness of the optimized isolation procedure.The extracted nuclear proteins were identified by 2-DE MS analyses,and a total of 748 proteins were identified.Bioinformatic analyses were performed to demonstrate the physicochemical properties,cellular locations and functions of the proteins. | ZHANG Yang FANG CaiYun BAO HuiMin FAN HuiZhi SHEN HuaLi YANG PengYuan | 2013 | Science China(Life Sciences)2013，56，6： | 3 |
| 3 | High expression of follicle stimulating hormone receptor in testicular tissue of idiopathic azoospermic patients with severe spermatogenic defects显示文摘 | Wang Liquan Huang Hefeng Jin Fan Zhou Caiyun Qian Yuli Chen Jianhua | 2014 | Chinese Medical Journal2014，，3： | 3 |
| 4 | Genetic mechanisms of the Jinchuan ultramafic intrusion and associated superlarge sulfide deposit, Northwest China显示文摘 | Xie Guanghong Wang Yunliang Fan Caiyun Zhang Chengjiang Zheng Rong | 1998 | Sci China (D)1998，，： | 1 |
| 5 | Population transcriptomics reveals a potentially positive role of expression diversity in adaptation显示文摘While it is widely accepted that genetic diversity determines the potential of adaptation,the role that gene expression variation plays in adaptation remains poorly known.Here we show that gene expression diversity could have played a positive role in the adaptation of Miscanthus lutarioriparius.RNA-seq was conducted for 80 individuals of the species,with half planted in the energy crop domestication site and the other half planted in the control site near native habitats.A leaf reference transcriptome consisting of 18,503 high-quality transcripts was obtained using a pipeline developed for de novo assembling with population RNA-seq data.The population structure and genetic diversity of M.lutarioriparius were estimated based on 30,609 genic single nucleotide polymorphisms.Population expression(Ep) and expression diversity(Ed)were defined to measure the average level and the magnitude of variation of a gene expression in the population,respectively.It was found that expression diversity increased while genetic Resediversity decreased after the species was transplanted from the native habitats to the harsh domestication site,especially for genes involved in abiotic stress resistance,histone methylation,and biomass synthesis under water limitation.The increased expression diversity could have enriched phenotypic variation directly subject to selections in the new environment. | Qin Xu Shilai Xing Caiyun Zhu Wei Liu Yangyang Fan Qian Wang Zhihong Song Wenhui Yang Fan Luo Fei Shang Lifang Kang Wenli Chen Juan Yan Jianqiang Li Tao Sang | 2015 | Journal of Integrative Plant Biology2015，57，3： | 1 |
| 6 | Using redundant parallel architecture to improve speaker recognition performance显示文摘In this paper, we propose two kinds of modifications in speaker recognition. First, the correlations between frequency channels are of prime importance for speaker recognition. Some of these correlations are lost when the frequency domain is divided into sub-bands. Consequently we propose a particularly redundant parallel architecture for which most of the correlations are kept. Second, generally a log transformation used to modify the power spectrum is done after the filter-bank in the classical spectrum calculation. We will see that performing this transformation before the filter bank is more interesting in our case. In the processing of recognition, the Gaussian mixture model (GMM) recognition arithmetic is adopted. Experiments on speech corrupted by noise show a better adaptability of this approach in noisy environments, compared with a conventional device, especially when pruning of some recognizers is performed. | Zhengquan QIU Junxun YIN Caiyun FAN | 2008 | 控制理论与应用(英文版)2008，6，2： | 1 |
| 7 | Dynamically modifiable wettability comparisons of the hydrophilic and hydrophobic substrates coated with F/TiO 2 hybrid sol by UV irradiation显示文摘 | Yunjie Yin Tao Li Fei Fan Caiyun Zhao Chaoxia Wang | 2013 | Applied Surface Science2013，，： | 1 |
| 8 | Non-ionizing radiofrequency field induces unfolded protein response (UPR) in endoplasmic reticulum of mouse neuronal cells显示文摘Objective:To examine whether exposure of mouse neuronal cells to radiofrequency fields used in mobile communication devices can induce stress in endoplasmic reticulum(ER)and activate unfolded protein response(UPR).Methods:HT22 mouse hippocampus neuronal cells were exposed to continuous wave 900 MHz radiofrequency fields(RF)at 120μW/cm2 power intensity for 4 h/d for 5 consecutive days.The positive control cells were irradiated with 4 Gy of 60Coγ-rays at a dose rate of 0.5 Gy/min(GR).Twenty-four hours after the last exposure,cells were collected,and the expressions of sensor transmembrane proteins were detected using Western blot analysis.Results:The expression levels of Ire1,PERK,p-IRE1 and p-PERK,GRP78 and CHOP proteins were detected.There were no statistically significant differences in the expression levels of IRE1 and PERK proteins in control(CT),sham(SH)-,RF-and GR-exposed cells(P<0.05).The phosphorylated protein levels of p-IRE1 and p-PERK were significantly increased in cells exposed to RF and GR(P<0.05).The expression levels of GRP78 and CHOP were significantly increased in RF-and GR-exposed cells compared to CT and SH-exposed cells(P<0.05).Cells treated with 1μg/ml TM for 24 h showed significantly increased expression levels of GRP78 and CHOP proteins compared to controls(P<0.05).In the presence of 2 mmol/L PBA,TM-induced increased levels of GRP78 and CHOP proteins were reduced(P<0.05).Conclusions:The exposure of non-ionizing 900 MHz RF was able to cause stress in HT22 mouse neuronal cells and activated UPR in ER.Since UPR plays an important role in both cell survival(when UPR is mitigated)and apoptosis/death(under unresolvable stress conditions),further studies are required to determine the fate of the cells exposed to RF. | Zhen Gao Wen Xie Caiyun Fan Yi Cao | 2020 | Radiation Medicine and Protection2020，1，3： | 1 |
| 9 | Influences of flexible defect on the interplay of supercoiling and knotting of circular DNA显示文摘Knots are discovered in biophysical systems,such as DNA and proteins.Knotted portions in knotted DNA are significantly bent and their corresponding bending angles are comparable with or larger than the sharp bending angle resulting in flexible defects.The role of flexible defects in the interplay of supercoiling and knotting of circular DNA were predicted by a Monte Carlo simulation.In knotted DNA with a particular knot type,a flexible defect noticeably enhances the supercoiling of the knotted DNA and the decreasing excitation energy makes the knotted portion more compact.A reduction in twist rigidity and unwinding of flexible defects are incorporated into the numerical simulations,so that interplay of supercoiling and knotting of circular DNA is studied under torsional conditions.Increasing unwinding not only results in a wider linking number distribution,but also leads to a drift of the distribution to lower values.A flexible defect has obvious effects on knotting probability.The summation of equilibrium distribution probability for nontrivial knotted DNA with different contour length does not change with excitation energy monotonically and has a maximum at an intermediate value of excitation energy around 5 kBT.In the phase space of knot length and gyration radius of knotted DNA,knot length does not anticorrelate with its gyration radius,which is attributed to the flexible defect in the knotted portion,which leads to the release of bending energy and inhibited the competition between entropy and bending energy. | Caiyun Xiong Xiaolin Nie Yixue Peng Xun Zhou Yangtao Fan Hu Chen Yanhui Liu | 2021 | Communications in Theoretical Physics2021，73，7： | 0 |
| 10 | ^(99m)Tc complex conjugated to insulin: CNS radiopharmaceuticals design based on principles of blood-brain barrier transport vector显示文摘Hydrophilic 99mTc-EC and nonlipophilic 99mTc- MAMA′-BA complexes, owing to the existing of intact blood-brain barrier (BBB) in vivo, cannot cross from blood to brain. Previous studies showed that insulin is selectively transported by receptor-mediated transcytosis through the brain capillary endothelial wall that makes up the BBB. In this paper, based on the characteristic of the insulin receptor enriched in brain capillary, the complexes of hydrophilic 99mTc-EC and nonlipophilic 99mTc-MAMA′-BA are conju- gated to insulin respectively. After purification, the radio- chemical purity of 99mTc-EC-insulin and 99mTc-MAMA′- BA-insulin was > 90% and the stability in vitro was good. Expectation for the special formulation can be internalized and endocytosed into the capillary membrane by the vec- tor-mediated brain delivery system, and transported 99mTc-labeled conjugate through the BBB in vivo, thus en- hancing brain uptake in mice. The biodistribution results of 99mTc-EC-insulin and 99mTc-MAMA′-BA-insulin in mice in- dicated that the brain uptake was higher than 99mTc-EC and 99mTc-MAMA′-BA to some extent. The ratios of brain uptake of 99mTc-EC-insulin to 99mTc-EC, 99mTc-MAMA′-BA-insulin to 99mTc-MAMA′-BA were 4―6 at 2 and 3 h post-injection respectively. In conclusion, the given results have illustrated a new way of brain uptake enhancing for nonlipophilic like complexes that have BBB delivery problems. It has a poten- tial value for the ongoing development of 99mTc-labeled ra- diopharmaceuticals for CNS receptors imaging. | LIU Fei FAN Caiyun ZHANG Jinming WANG Wushang LIU Boli | 2005 | Chinese Science Bulletin2005，50，20： | 0 |
| 11 | The protective effect of cyclodextrin on the color quality and stability of Cabernet Sauvignon red wine显示文摘The impact of cyclodextrins(CDs)on wine quality and stability remains largely unknown.This study systematically assessed the protective effect of the post-fermentation addition of CDs on color stability of red wine from the viewpoints of color characteristics,copigmentation and phenolic profiles.The grey relational analysis(GRA)and principal component analysis(PCA)methods were employed to dissect the key effective determinants related to color quality.The addition of CDs induced a significant hyperchromic effect of 8.19-25.40%,a significant bathochromic effect and an enhancement of the color intensity.Furthermore,the evolution of anthocyanin forms and the content of monomeric anthocyanins revealed that β-CD is a superior favorable cofactor during wine aging,but for long-term aging,2-HP-β-CD and 2-HP-γ-CD are more beneficial in promoting the formation of polymerized anthocyanins and color stability.This work provides an important reference for the use of CDs to enhance the color quality and stability of red wines. | Caiyun Liu Lulu Wu Shuyue Fan Yongsheng Tao Yunkui Li | 2024 | Journal of Integrative Agriculture2024，23，1： | 0 |
| 12 | Synthesis of Novel Bifunctional Chelator for (177)~Lu Labelling显示文摘The research of(177)^Lu labelled radiopharmaceuticals attracted much attention in recent years.The most widely used bifunctional chelators are DOTA and DTPA.However,the labelling procedure of DOTA needs heating(40-90℃)and extends reaction time(30-180min),and these conditions are typically required for quantitative radiolabelling,which can be incompatible with antibodies and other biomolecules.DTPA will successfully radiolabel these isotopes in 10-15min at ambient temperature.However,the in vivo stability is less optimal than that of DOTA.So it’s important to develop novel bifunctional chelator with better properties for the labelling of 177Lu. | FAN Caiyun WANG Yue LI Fenglin XIE Qinghua LIU Zihua | 2018 | 中国原子能科学研究院年报2018，，1： | 0 |
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