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| 1 | Identification of immunoreactive proteins of Brucella melitensis by immunoproteomics显示文摘Infection with Brucella causes brucellosis, a chronic disease in humans, which induces abortion and sterility in livestock. Among the different Brucella species, Brucella melitensis is considered the most virulent and is the predominant species associated with outbreaks in China. To date, no safe human vaccine is available against Brucella infection. The currently used live vaccines against Brucella in livestock induce antibodies that interfere with the diagnosis of field infection in vaccinated animals, which is harmful to eradication programs. However, there is as yet no complete profile of immunogenic proteins of B. melitensis. Towards the development of a safer, equally efficacious, and field infection-distinguishable vaccine, we used immunoproteomics to identify novel candidate immunogenic proteins from B. melitensis M5. Eighty-eight immunoreactive protein spots from B. melitensis M5 were identified by Western blotting and were assigned to sixty-one proteins by mass spectrometry, including many new immunoreactive proteins such as elongation factor G, F0F1 ATP synthase subunit beta, and OMP1. These provide many candidate immunoreactive proteins for vaccine development. | ZHAO ZhongPeng YAN Fang JI WenHui LUO DeYan LIU Xin XING Li DUAN YueQiang YANG PengHui SHI XiuMin LI Zhong WANG XiLiang | 2011 | Science China(Life Sciences)2011，54，9： | 12 |
| 2 | High-Resolution Genome-wide Association Study Identifies Genomic Regions and Candidate Genes for Important Agronomic Traits in Wheat显示文摘Wheat(Triticum aestivum)is a major staple food crop worldwide.Genetic dissection of important agronomic traits is essential for continuous improvement of wheat yield to meet the demand of the world's growing population.We conducted a large-scale genome-wide association study(GWAS)using a panel of 768 wheat cultivars that were genotyped with 327609 single-nucleotide polymorphisms generated by genotyping-by-sequencing and detected 395 quantitative trait loci(QTLs)for 12 traits under 7 environments.Among them,273 QTLs were delimited to≤1.0-Mb intervals and 7 of them are either known genes(Rht-D,Vrn-B1,and Vrn-D1)that have been cloned or known QTLs(TaGA2ox8,APO1,TaSus1-7B,and Rht12)that were previously mapped.Eight putative candidate genes were identified for three QTLs that enhance spike seed setting and grain size using gene expression data and were validated in three bi-parental populations.Protein sequence analysis identified 33 putative wheat orthologs that have high identity with rice genes in QTLs affecting similar traits.Large r^2 values for additive effects observed among the QTLs for most traits indicated that the phenotypes of these identified QTLs were highly predictable.Results from this study demonstrated that significantly increasing GWAS population size and marker density greatly improves detection and identification of candidate genes underlying a QTL,solidifying the foundation for large-scale QTL fine mapping,candidate gene validation,and developing functional markers for genomics-based breeding in wheat. | Yunlong Pang Chunxia Liu Danfeng Wang Paul St.Amand Amy Bernardo Wenhui Li Fang He Linzhi Li Liming Wang Xiufang Yuan Lei Dong Yu Su Huirui Zhang Meng Zhao Yunlong Liangi Hongze Jia Xitong Shen Yue Lu Hongming Jiang Yuye Wu Anfei Li Honggang Wang Lingrang Kong Guihua Bai Shubing Liu | 2020 | Molecular Plant2020，13，9： | 8 |
| 3 | AFM research on Fe-based nanocrystal crystallization mechanism显示文摘The cross-section pattern of Fe-based alloy ribbon (Fe73.5Cu1Nb3Si13.5B9) annealed at different temperatures was investigated by AFM (atomic force microscope), and the effect mechanism of Nb and Cu in Fe-based alloy ribbon annealing was analyzed with XRD diffraction crystal analysis technique and other research results. New concepts of encapsulated grain, Nb vacancy cluster, Nb-B atom cluster and so on were proposed and used to describe the formation mechanism of α-Fe (Si) nanocrystal. Finally, a three-phase (separation phase, encapsulated phase and nanocrystalline phase) interconnected structure model in Fe-based nanocrystalline alloy was established. | FANG YunZhang WU FengMin ZHENG JinJu SHI FangYe WU WenHui SUN HuaiJun LIN GenJin MAN QiKui HU JuanMei YANG XiaoHong | 2008 | Science China(Technological Sciences)2008，51，1： | 8 |
| 4 | Quantitative single-molecule study of TGF-β/ Smad signaling显示文摘TGF-/Smad 发信号小径在不同房间类型和环境条件之中触发多样的细胞的回答。相互作用在 TGF-/Smad 包含了发信号的蛋白质蛋白质的定量分析为理解这条发信号的小径的分子的机制被要求。有高空间与时间的分辨率的实时房间的单个分子的缩微本是一个新工具以一种即时方式监视关键分子的事件。在这评论,我们主要在由单个分子的方法表明蛋白质的 TGF-/Smad 的量的描述上介绍了最近的工作,并且显示出它怎么使我们能关于这个正规发信号过程获得新卓见。 | Rong Zhao Nan Li Jiachao Xu Wenhui Li Xiaohong Fang | 2018 | Acta Biochimica et Biophysica Sinica2018，50，1： | 5 |
| 5 | Effects and molecular mechanisms of AT1-AA in retinopathy of preeclampsia显示文摘Preeclampsia not only seriously endangers maternal and fetal health during pregnancy but may incur many sequelae in postpartum women such as reduced visual acuity.Agonistic autoantibodies to the angiotensin Ⅱ type Ⅰ receptor (AT1-AA) is closely associated with preeclampsia.The aim of the present study is to determine whether AT1-AA is associated with retinal impairment during the course of preeclampsia.A preeclampsia model was established by injecting AT1-AA into pregnant rats via the tail vein.Changes in the retinal histological structure were observed.Cell apoptosis and cytokines including reactive oxygen species (ROS),as well as apoptosis-related proteins such as Bcl-2,Bax,and caspase-3 were detected.In addition,flash electroretinograms obtained at different postpartum days were analyzed.Compared with the control group,the retinal structure became edematous and the cell density was reduced significantly in preeclampsia group.The cell apoptosis rate was increased significantly.In addition,the content of ROS,the levels of Bax and caspase-3 in the retina were increased,while the content of Bcl-2 was reduced significantly.Continuous observation of the electroretinograms showed loss of retinal ganglion cells postpartum.The present study demonstrated that AT1-AA induced retinal cell apoptosis by promoting ROS release and activating caspase,suggesting that the increased postpartum susceptibility of preeclamptic women to retinopathy is related to AT1-AA-induced cell apoptosis. | Fang Liu Lei Yang Yanqian Zheng Wenhui Zhang Jianming Zhi | 2019 | Acta Biochimica et Biophysica Sinica2019，51，1： | 3 |
| 6 | Analysis of F_1 hybrid and BC_1 monosomic alien addition line plants from Brassica oleracea × Sinapis alba by GISH显示文摘Sterile and semi-fertile F_1 plants were obtained by intergeneric sexual hybridization between paternal Brassica oleracea var. alboglabra (genome CC, 2n=18) and maternal Sinapis alba (genome SS, 2n=24), BC_1 plants were obtained by backcrossing between paternal B. oleracea and ma-ternal semi-fertile F_1 plants. Genomic in situ hybridization (GISH) combined with dual-colour fluorescence in situ hybridization (dcFISH) showed that sterile F_1 plants contained 21 chromosomes consist-ing of one B. oleracea chromosome set and one S. alba chromosome set, belonging to expected hybrids, and semi-fertile F_1 plants contained 30 chromosomes consisting of two B. oleracea chromosome sets and one S. alba chromosome set. It is obvious that the semi-fertile F_1 plants belong to unexpected hybrids. 1―3 trivalents were detected at meiotic metaphase I of semi-fertile F_1 pollen mother cells (PMCs). Different separation ratios of S chromosomes were detected at anaphase I. A monosomic alien addition line (MAAL) was identified by GISH-dcFISH from BC_1 plants; it contained 19 chromosomes consisting of 18 C chromosomes and 1 S chromosome. At meiotic metaphase I, 9 divalents from B. oleracea and one univalent from S. alba could be detected. Sometimes, one putative C-S trivalent could also be detected. The achievement of B. oleracea-S. alba monosomic alien addition lines lays a foundation for gene introgression, location and cloning. | WEI Wenhui ZHANG Sufeng LI Jun WANG Lijun CHEN Bo FANG Xiaoping WANG Zhuan LUO Lixia | 2006 | Chinese Science Bulletin2006，51，23： | 3 |
| 7 | Precast system and assembly connection of cement concrete slabs for road pavement: A review显示文摘Prefabricated pavement is increasingly applied worldwide due to the rapid construction on-site.This paper presents a state-of-the-art review of the precast systems and assembly connection of concrete slabs,as well as the precast pavement features.The previous research indicates that:(1)both super-slab and Michigan systems are recommended with the satisfied road performance close to the cast-in-place;(2)flexible base material is suggested for the fabricated pavement for the satisfied leveling and stress distribution;(3)to prevent the voiding phenomenon of the fabricated pavement,the sand cushion course,dry mixed mortar or self-leveling mortar,and other flowable materials could be used for the secondary leveling of the base after the pavement splicing;(4)two-direction dowel bars are recommended for slab connections of the fabricated pavement,which helps to improve the load transfer capacity of the joints and enhance the durability of the fabricated pavement structure;(5)the sealing treatment of precast slab joints needs strengthening to reduce the impact of surface runoff on the base course;(6)the further research focuses are designing with functional,composite,mechanized,intelligent,lightweight,and flexible pavement slabs.Besides,pavement mechanical properties induced by temperature overlapping traffic loads need to be revealed. | Mingjing Fang Ran Zhou Wenhui Ke Bo Tian Yubin Zhang Jianjun Liu | 2022 | Journal of Traffic and Transportation Engineering(English Edition)2022，9，2： | 3 |
| 8 | SRSF1 plays a critical role in invariant natural killer T cell development and function显示文摘Invariant natural killer T(iNKT)cells are highly conserved innate-like T lymphocytes that originate from CD4^(+)CD8^(+)double-positive(DP)thymocytes.Here,we report that serine/arginine splicing factor 1(SRSF1)intrinsically regulates iNKT cell development by directly targeting Myb and balancing the abundance of short and long isoforms.Conditional ablation of SRSF1 in DP cells led to a substantially diminished iNKT cell pool due to defects in proliferation,survival,and TCRαrearrangement.The transition from stage 0 to stage 1 of iNKT cells was substantially blocked,and the iNKT2 subset was notably diminished in SRSF1-deficient mice.SRSF1 deficiency resulted in aberrant expression of a series of regulators that are tightly correlated with iNKT cell development and iNKT2 differentiation,including Myb,PLZF,Gata3,ICOS,and CD5.In particular,we found that SRSF1 directly binds and regulates pre-mRNA alternative splicing of Myb and that the expression of the short isoform of Myb is substantially reduced in SRSF1-deficient DP and iNKT cells.Strikingly,ectopic expression of the Myb short isoform partially rectified the defects caused by ablation of SRSF1.Furthermore,we confirmed that the SRSF1-deficient mice exhibited resistance to acute liver injury uponα-GalCer and Con A induction.Our findings thus uncovered a previously unknown role of SRSF1 as an essential post-transcriptional regulator in iNKT cell development and functional differentiation,providing new clinical insights into iNKT-correlated disease. | Jingjing Liu Menghao You Yingpeng Yao Ce Ji Zhao Wang Fang Wang Di Wang Zhihong Qi Guotao Yu Zhen Sun Wenhui Guo Juanjuan Liu Shumin Li Yipeng Jin Tianyan Zhao Hai-Hui Xue Yuanchao Xue Shuyang Yu | 2021 | Cellular & Molecular Immunology2021，18，11： | 3 |
| 9 | Influence of amplified spontaneous emission and fluorescence of β-carotene on stimulated Raman scattering of carbon disulfide显示文摘β-carotene with double fluorescence characteristics and large third-order optical nonlinearities,which is dissolved in the carbon disulfide(CS2) as the core medium of a liquid core optical fiber(LCOF),is applied in the study of the CS2 stimulated Raman scattering(SRS). The results of this study show that when the concentrations of solution are more than 3.72×10-7 mol/L,the amplified spontaneous emis-sion(ASE) of β-carotene is the main factor influencing the threshold and intensity of Stokes lines; when the concentrations of solution are lower than 3.72×10-7 mol/L,the ASE disappears and the fluorescence plays the key role:The high-order Stokes lines may be observed at very low input-laser power,and the Stokes thresholds decrease as the solution concentration increases. The result may be widely used in the study of broadband stimulated radiation laser and seeding laser. | MEN ZhiWei SUN XiuPing FANG WenHui LI ZuoWei CAO Biao GAO ShuQin LU GuoHui | 2009 | Science China(Physics,Mechanics & Astronomy)2009，52，4： | 3 |
| 10 | Mechanism of agonistic angiotensin Ⅱ type Ⅰ receptor autoantibody-amplified contractile response to Ang Ⅱ in the isolated rat thoracic aorta显示文摘 | Wenhui Zhang Yanqian ZhenG Fang Liu Xiaofang Wang Zhu Jin Jianming Zhi | 2015 | Acta Biochimica et Biophysica Sinica2015，47，10： | 2 |
| 11 | Bi_(2)O_(2)Se/BP van der Waals heterojunction for high performance broadband photodetector显示文摘Broadband photodetector has wide applications in the field of remote sensing,health monitoring and medical imaging.Two-dimensional(2D)materials with narrow bandgaps have shown enormous potential in broadband photodetection.However,the device performance is often restricted by the high dark currents.Herein,we demonstrate a high performance broadband photodetector by constructing Bi_(2)O_(2) Se/BP van der Waals heterojunction.The device exhibits a p-n diode behavior with a current rectification ratio of~20.Benifited from the low dark current of the heterojunction and the effective carrier separation,the device achieves the responsivity(R)of~500 A/W,~4.3 A/W and~2.3 A/W at 700 nm,1310 nm and 1550 nm,respectively.The specific detectivity(D^(*))is up to~2.8×10^(11) Jones(700 nm),~2.4×10^(9) Jones(1310 nm)and~1.3×10^(9) Jones(1550 nm).Moreover,the response time is~9 ms,which is more than 20 times faster than that of individual BP(~190 ms)and Bi_(2)O_(2)Se(~180 ms)devices. | Xing LIU Wenhui WANG Fang YANG Shaopeng FENG Zhenliang HU Junpeng LU Zhenhua NI | 2021 | Science China(Information Sciences)2021，64，4： | 2 |
| 12 | Lipase-catalyzed synthesis of aliphatie polyesters and properties characterization显示文摘 | LIU Wenhui CHEN Biqiang WANG Fang | 2011 | Process Biochem2011，46，10： | 1 |
| 13 | Investigation on the micelle-sen- sitized Ce(IV)-lomoxicam-Rh B chemiluminescence sys- tem and its application显示文摘 | Fang Zhao Wenhui Zhao | 2012 | J Fluoresc2012，22，： | 1 |
| 14 | Growth profile of stimulated Raman anti-Stokes scattering influenced by fluorescence seeding in liquid-core optical fiber 显示文摘 | Men zhiwei Fang wenhui Li zuowei | 2010 | J Raman Spectroscopy2010，41，： | 1 |
| 15 | TCF-1 deficiency influences the composition of intestinal microbiota and enhances susceptibility to colonic inflammation显示文摘Dear Editor,The diversity and composition of gut microbiota play critical roles for maintaining the homeostasis of host commensal bacteria which are correlated with mammalian health(Ding and Schloss,2014).Disorder of microbial community is implicated in the pathogenesis of several human diseases,such as autoimmune disease,allergy,diabetes,obesity,and inflammatory bowel disease(IBD),which is clearly demonstrated to relate to gut dysbiosis in patients and mouse models(Saleh and Elson,2011). | Guotao Yu Fang Wang Menghao You Tiansong Xu Chunlei Shao Yuning Liu Ruiqi Liu Min Deng Zhihong Qi Zhao Wang Jingjing Liu Yingpeng Yao Jingjing Chen Zhen Sun Shanshan Hao Wenhui Guo Tianyan Zhao Zhengquan Yu Qian Zhang Yaofeng Zhao Feng Chen Shuyang Yu | 2020 | Protein & Cell2020，11，5： | 1 |
| 16 | H_(2)O_(2) actuated molybdenum oxide nanodots:Multi-enzyme-like activities,leverage of Fenton reaction,and dual-mode sensitive detection of alendronate sodium显示文摘Unexpected benefits to the catalytic performance of materials often originate from the presence of surface defects.Here,novel Dpenicillamine modified molybdenum oxide nanodots,with abundant oxygen vacancy defects,were fabrication by a mild,simple,and cost-effective method.Ultraviolet–visible(UV–Vis)absorption spectra analysis showed that the nanodots had peroxidaselike and catalase-like activities.The reactive oxygen species were probed by electronic paramagnetic resonance technique and spectroscopic methods,demonstrating that the nanodots also had oxidase-like activity.Interestingly,the peroxidase-like activity of nanodots was synergistically enhanced in the presence of ferrous ions or ferric ions.Remarkably,less than nanomolar levels of ferrous ions were required to display this phenomenon,meaning Fenton reagent acted as leverage.Based on this,a sensitive colorimetric and fluorescent dual-mode sensor for alendronate sodium was developed.The linear ranges for colorimetric and fluorescence analysis were 0.2–2.5 and 0.2–2.0μM,with detection limits of 31.21 and 71.84 nM,correspondingly.The method has a simple large-scale material preparation process with higher sensitivity and shorter reaction time,which can inspire and enlighten the design of nanozyme sensors. | Siqi Wang Yao Jin Wenhui Ai Xiufeng Wang Zhiqing Zhang Ting Zhou Guodong Zhang Fang Wang | 2023 | Nano Research2023，16，10： | 1 |
| 17 | The significance of changes in high mobility group - 1 protein mRNA expression in rats after thermal injury显示文摘 | Fang Wenhui Yao Yongming Shi Zhiguo | 2002 | Shock2002，17，： | 1 |
| 18 | Magnitude and Forming Factors of Mass Elevation Effect on Qinghai-Tibet Plateau显示文摘Mass elevation effect(MEE) refers to the thermal effect of huge mountains or plateaus, which causes the tendency for temperature-related montane landscape limits to occur at higher elevations in the inner massifs than on their outer margins. MEE has been widely identified in all large mountains, but how it could be measured and what its main forming-factors are still remain open. This paper, supposing that the local mountain base elevation(MBE) is the main factor of MEE, takes the Qinghai-Tibet Plateau(QTP) as the study area, defines MEE as the temperature difference(ΔT) between the inner and outer parts of mountain massifs, identifies the main forming factors, and analyzes their contributions to MEE. A total of 73 mountain bases were identified, ranging from 708 m to 5081 m and increasing from the edges to the central parts of the plateau. Climate data(1981–2010) from 134 meteorological stations were used to acquire ΔT by comparing near-surface air temperature on the main plateau with the free-air temperature at the same altitude and similar latitude outside of the plateau. The ΔT for the warmest month is averagely 6.15℃, over 12℃ at Lhatse and Baxoi. A multivariate linear regression model was developed to simulate MEE based on three variables(latitude, annual mean precipitation and MBE), which are all significantly correlated to ΔT. The model could explain 67.3% of MEE variation, and the contribution rates of three independent variables to MEE are 35.29%, 22.69% and 42.02%, respectively. This confirms that MBE is the main factor of MEE. The intensive MEE of the QTP pushes the 10℃ isotherm of the warmest month mean temperature 1300–2000 m higher in the main plateau than in the outer regions, leading the occurrence of the highest timberline(4900 m) and the highest snowline(6200 m) of the Northern Hemisphere in the southeast and southwest of the plateau, respectively. | ZHANG Shuo ZHANG Baiping YAO Yonghui ZHAO Fang QI Wenwen HE Wenhui WANG Jing | 2016 | Chinese Geographical Science2016，26，6： | 1 |
| 19 | 6 - 18 Operation Status of On-line Ion Sources in 2012显示文摘 | FengYucheng Lu Wang Li Jinyu Zhang Wenhui Ma Hongyi Ma Baohua Wang Hui Li Jiaqing Lin Shuhao Cao Yun Sha Shan W u Qi Guo Junwei Fang Xing Yang Yao Li Xixia Zhang Hengjuan Zhao Huanyu Zhang Xuezhen Sun Liangting Zhao Hongwei | 2012 | IMP & HIRFL Annual Report2012，，1： | 1 |
| 20 | Visualizing the melting processes in ultrashort intense laser triggered gold mesh with high energy electron radiography显示文摘High-energy electron radiography(HEER)is a promising diagnostic tool for high-energy-density physics,as an alternative to tools such as X/γ-ray shadowgraphy and high-energy proton radiography.Impressive progress has been made in the development and application ofHEER in the past fewyears,and its potential for high-resolution imaging of static opaque objects has been proved.In this study,by taking advantage of the short pulse duration and tunable time structure of high-energy electron probes,time-resolved imaging measurements of high-energy-density gold irradiated by ultrashort intense laser pulses are performed.Phenomena at different time scales frompicoseconds to microseconds are observed,thus proving the feasibility of this technique for imaging of static and dynamic objects. | Zheng Zhou Yu Fang Han Chen Yipeng Wu Yingchao Du Zimin Zhang Yongtao Zhao Ming Li Chuanxiang Tang Wenhui Huang | 2019 | Matter and Radiation at Extremes2019，4，6： | 1 |
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