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| 1 | Integrative genome-wide analysis reveals HLP1, a novel RNA-binding protein, regulates plant flowering by targeting alternative polyadenylation显示文摘 | Yong Zhang Lianfeng Gu Yifeng Hou Lulu Wang Xian Deng Runlai Hang Dong Chen Xiansheng Zhang Yi Zhang Chunyan Liu Xiaofeng Cao | 2015 | Cell Research2015，25，7： | 11 |
| 2 | Label-free fiber nanograting sensor for real-time in situ early monitoring of cellular apoptosis显示文摘The achievement of functional nanomodules for subcellular label-free measurement has long been pursued in order to fully understand cellular functions.Here,a compact label-free nanosensor based on a fiber taper and zinc oxide nanogratings is designed and applied for the early monitoring of apoptosis in individual living cells.Because of its nanoscale dimensions,mechanical flexibility,and minimal cytotoxicity to cells,the sensing module can be loaded in cells for long term in situ tracking with high sensitivity.A gradual increase in the nuclear refractive index during the apoptosis process is observed,revealing the increase in molecular density and the decrease in cell volume.The strategy used in our study not only contributes to the understanding of internal environmental variations during cellular apoptosis but also provides a new platform for nonfluorescent fiber devices for investigation of cellular events and understanding fundamental cell biochemical engineering. | Danran Li Nina Wang Tianyang Zhang Guangxing Wu Yifeng Xiong Qianqian Du Yunfei Tian Weiwei Zhao Jiandong Ye Shulin Gu Yanqing Lu Dechen Jiang Fei Xu | 2022 | Advanced Photonics2022，4，1： | 5 |
| 3 | Time Domain Calculation of Connector Loads of a Very Large Floating Structure显示文摘在飞机坠毁,轮船碰撞,和另外的事故以后产生的负担可以破坏很大的漂浮的结构(VLFS ) 并且创造另外的接头负担。在这研究,负担被考虑建立的轮船碰撞和波浪的联合效果打手势为多身体 VLFS 的微分方程。一个时间领域计算方法被建议在波浪计算 VLFS 的接头负担。LonguetHiggins 模特儿被雇用模仿随机的波浪负担。液体力量和水动力学系数用 DNV Sesam 软件被获得。运动当频率域水动力学系数被变换成运动的存储器函数时,微分方程被使用时间域方法计算时间域的微分方程。由于波浪和影响负担的联合行动,高周波的摆动在接头负担的时间历史曲线被观察。在 0 的波浪方向? | Jiayang Gu Jie Wu Enrong Qi Yifeng Guan Yubo Yuan | 2015 | Journal of Marine Science and Application2015，14，2： | 3 |
| 4 | The promoting effect of low-level sulfidation in PdCuS nanoparticles catalyzed alkyne semihydrogenation显示文摘硫来源的支持的效果是吸引人却糟糕听说的现象。此处,我们与硫粉末的不同数量学习了 PdCu 二金属的 nanoparticles (NP ) 的处理。低级 sulfidation 导致了由表面组成的 Pd30Cu10S9 NP 催化剂的产生充实的 Pd NP,电子缺乏的 Pd 和 Cu,以及零原子价硫。Pd30Cu10S9 NP 催化剂在炔属羟的 semihydrogenation 显示出显着地提高的活动和选择。我们的学习第一次在原子水平为硫的支持的效果揭示了一个可能的原因,建议在催化剂设计的新策略。 | Cai Wu Yifeng Chen Rongan Shen Wei Zhu Yue Gong Lin Gu Qing Peng Huifang Guo Wei He | 2018 | Nano Research2018，11，9： | 2 |
| 5 | Positive association of the human GABA-A-receptor beta 2 subunit gene haplotype with schizophrenia in the Chinese Han population显示文摘 | Jixia Liu Yongyong Shi Wei Tang Tingwei Guo Dawei Li Yifeng Yang Xinzhi Zhao Hongsheng Wang Xingwang Li Guoyin Feng Niufan Gu Shaomin Zhu Huijun Liu Yangling Guo Jianguo Shi Hong Sang Lijuan Yan Lin He | 2005 | Biochemical and Biophysical Research Communications2005，，3： | 1 |
| 6 | All-optical demultiplexing of WLAN and cellular CDMA radio signals显示文摘 | Hatice Kosek Yifeng He Xijia Gu | | 0，，06： | 1 |
| 7 | Effect of Sm and Mg co-doping on the properties of ceria-based electrolyte materials for IT-SOFCs显示文摘 | Yifeng Zheng Haitao Gu Han Chen Ling Gao Xiaofang Zhu Lucun Guo | 2008 | Materials Research Bulletin2008，，4： | 1 |
| 8 | Investigation of the expression patterns and correlation of DNA methyltransferasesand class I histone deacetylases in ovarian cancer tissues显示文摘 | Yifeng Gu Peifang Yang Qing Shao Xia Liu Sheng Xia Miaomiao Zhang Huaxi Xu Qixiang Shao | 2013 | Oncology Letters2013，，2： | 1 |
| 9 | Defect-rich and ultrathin nitrogen-doped carbon nanosheets with enhanced peroxidase-like activity for the detection of urease activity and fluoride ion显示文摘Although carbon nanozymes have attracted great interest due to their good biocompatibility, low cost,and high stability, designing high-active carbon nanozymes still faces great challenges. Herein, ultrathin nitrogen-doped carbon nanosheets with rich defects(d-NC) were prepared through a high-temperature annealing process, using potassium chloride and ammonium chloride as templates. Owing to the large specific surface area, rich defects and the high exposure of active sites, the proposed d-NC nanozymes exhibited excellent peroxidase-like activity. The d-NC nanozymes possess maximal reaction velocity and their specific activity is 9.4-fold higher than that of nitrogen-doped carbon nanozymes, indicating that the induced defects can boost the catalytic performance. Benefited from the good peroxidase-like activities of d-NC nanozymes, the colorimetric sensing platforms were constructed for the detection of urease activity and fluoride ion, exhibiting satisfactory stability and selectivity. This study not only offers a way to synthesize carbon nanozymes with improved enzyme-like activities but also broadens their applications in colorimetric biosensing. | Yu Zhang Lei Jiao Weiqing Xu Yifeng Chen Yu Wu Hongye Yan Wenling Gu Chengzhou Zhu | 2022 | Chinese Chemical Letters2022，33，3： | 1 |
| 10 | In situ synthesis and unidirectional insertion of membrane proteins in liposome-immobilized silica stationary phase for rapid preparation of microaffinity chromatography显示文摘Cell membrane affinity chromatography has been widely applied in membrane protein(MP)-targeted drug screening and interaction analysis.However,in current methods,the MP sources are derived from cell lines or recombinant protein expression,which are time-consuming for cell culture or purification,and also difficult to ensure the purity and consistent orientation of MPs in the chromatographic stationary phase.In this study,a novel in situ synthesis membrane protein affinity chromatography(iSMAC)method was developed utilizing cell-free protein expression(CFE)and covalent immobilized affinity chromatography,which achieved efficient in situ synthesis and unidirectional insertion of MPs into liposomes in the stationary phase.The advantages of iSMAC are:1)There is no need to culture cells or prepare recombinant proteins;2)Specific and purified MPs with stable and controllable content can be obtained within 2 h;3)MPs maintain the transmembrane structure and a consistent orientation in the chromatographic stationary phase;4)The flexible and personalized construction of cDNAs makes it possible to analyze drug binding sites.iSMAC was successfully applied to screen PDGFRβinhibitors from Salvia miltiorrhiza and Schisandra chinensis.Micro columns prepared by in-situ synthesis maintain satisfactory analysis activity within 72 h.Two new PDGFRβinhibitors,salvianolic acid B and gomisin D,were screened out with KD values of 13.44 and 7.39μmol/L,respectively.In vitro experiments confirmed that the two compounds decreasedα-SMA and collagen Ⅰ mRNA levels raised by TGF-βin HSC-T6 cells through regulating the phosphorylation of p38,AKT and ERK.In vivo,Sal B could also attenuate CCl_(4)-induced liver fibrosis by downregulating PDGFRβdownstream related protein levels.The iSMAC method can be applied to other general MPs,and provides a practical approach for the rapid preparation of MP-immobilized or other biological solid-phase materials. | Yanqiu Gu Rong Wang Panpan Chen Shengnan Li Xinyi Chai Chun Chen Yue Liu Yan Cao Diya Lv Zhanying Hong Zhenyu Zhu Yifeng Chai Yongfang Yuan Xiaofei Chen | 2022 | Acta Pharmaceutica Sinica B2022，12，9： | 1 |
| 11 | La and Ca co-doped ceria-based electrolyte materials for IT-SOFCs显示文摘 | ZHENG Yifeng SHI Yueming GU Haitao | 2009 | Mater Res Bull2009，44，： | 1 |
| 12 | A Novel Weightedgraph-based Grouping Algorithm for Metadata Prefetching显示文摘 | Gu Peng Wang Jun Zhu Yifeng | 2010 | IEEE Transactions on Computers2010，59，1： | 1 |
| 13 | La and Ca co-doped ceria-based electrolyte materials for IT-SOFCs 显示文摘 | Zheng Yifeng Shi Yueming Gu Haitao | 2009 | Materials Research Bulletin2009，44，： | 1 |
| 14 | Advantages of Si, Sbz Te phase-change material and its applications in phase?change random access memory显示文摘 | Gu Yifeng Cheng Yan | 2011 | Sci pta Materilia2011，65，： | 1 |
| 15 | Screening of immune cell activators from Astragali Radix using a comprehensive two-dimensional NK-92MI cell membrane chromatography/C18 column/time-of-flight mass spectrometry system显示文摘Astragali Radix(AR)is a clinically used herbal medicine with multiple immunomodulatory activities that can strengthen the activity and cytotoxicity of natural killer(NK)cells.However,owing to the complexity of its composition,the specific active ingredients in AR that act on NK cells are not clear yet.Cell membrane chromatography(CMC)is mainly used to screen the active ingredients in a complex system of herbal medicines.In this study,a new comprehensive two-dimensional(2D)NK-92MI CMC/C18 column/time-of-flight mass spectrometry(TOFMS)system was established to screen for potential NK cell activators.To obtain a higher column efficiency,3-mercaptopropyltrimethoxysilane-modified silica was synthesized to prepare the NK-92MI CMC column.In total,nine components in AR were screened from this system,which could be washed out from the NK-92MI/CMC column after 10 min,and they showed good affinity for NK-92MI/CMC column.Two representative active compounds of AR,isoastragaloside Ⅰ and astragaloside IV,promoted the killing effect of NK cells on K562 cells in a dose-dependent manner.It can thus suggest that isoastragaloside Ⅰ and astragaloside Ⅳ are the main immunomodulatory components of AR.This comprehensive 2D NK-92MI CMC analytical system is a practical method for screening immune cell activators from other herbal medicines with immunomodulatory effects. | Xinyi Chai Yanqiu Gu Lei Lv Chun Chen Fei Feng Yan Cao Yue Liu Zhenyu Zhu Zhanying Hong Yifeng Chai Xiaofei Chen | 2022 | Journal of Pharmaceutical Analysis2022，12，5： | 0 |
| 16 | PHASE DIAGRAMS OF SYSTEMS SmCl_3-CaCl_2 AND SmCl_3-LiCl显示文摘The phase diagrams of binary systems SmCl3-CaCl2 and SmCl3-LiCl were determined byDTA and X-ray diffraction analysis.The system SmCl3-CaCl2 is of a simple eutectic typewith eutectic point of 55.8mol-% SmCl3 at 545℃.The system SmCl3-LiCl is of the typewith incongruent melting phase 2SmCl3·LiCl at peritectic point 434℃ and with eutecticpoint of 31.5 mol-% SmCl3 at 395℃. | ZHANG Yifeng MENG Bo GU Yunyan Northeast University of Technology,Shenyang,China Professor,Department of Chemistry,Northeast University of Technology,Shenyang 110006,China | 1992 | Acta Metallurgica Sinica(English Letters)1992，5，9： | 0 |
| 17 | Fine-Tuning Pyridinic Nitrogen in Nitrogen-Doped Porous Carbon Nanostructures for Boosted Peroxidase-Like Activity and Sensitive Biosensing显示文摘Carbon materials have been widely used as nanozymes in bioapplications,attributing to their intrinsic enzyme-like activities.Nitrogen(N)-doping has been explored as a promising way to improve the activity of carbon material-based nanozymes(CMNs).However,hindered by the intricate N dopants,the real active site of N-doped CMNs(N-CMNs)has been rarely investigated,which subsequently retards the further progress of high-performance N-CMNs.Here,a series of porous N-CMNs with well-controlled N dopants were synthesized,of which the intrinsic peroxidase(POD)like activity has a positive correlation with the pyridinic N content.Density functional theory calculations also reveal that pyridinic N boosts the intrinsic POD-like activity of N-CMNs.Pyridinic-N dopant can effectively promote the first H_(2)O desorption process in comparison with the graphitic and pyrrolic N,which is the key endothermic reaction during the catalytic process.Then,utilizing the optimized nanozymes with high pyridinic N content(NP-CMNs)and superior POD-like activity,a facile total antioxidant capacity(TAC)assay was developed,holding great promise in the quality assessment of medicine tablets and antioxidant food for healthcare and healthy diet. | Hongye Yan Linzhe Wang Yifeng Chen Lei Jiao Yu Wu Weiqing Xu Wenling Gu Weiyu Song Dan Du Chengzhou Zhu | 2020 | Research2020，，1： | 0 |
| 18 | Deep Learning Trackers Review and Challenge显示文摘Recently,deep learning has achieved great success in visual tracking.The goal of this paper is to review the state-of-the-art tracking methods based on deep learning.First,we categorize the existing deep learning based trackers into three classes according to network structure,network function and network training.For each categorize,we analyze papers in different categories.Then,we conduct extensive experiments to compare the representative methods on the popular OTB-100,TC-128 and VOT2015 benchmarks.Based on our observations.We conclude that:(1)The usage of the convolutional neural network(CNN)model could significantly improve the tracking performance.(2)The trackers with deep features perform much better than those with low-level hand-crafted features.(3)Deep features from different convolutional layers have different characteristics and the effective combination of them usually results in a more robust tracker.(4)The deep visual trackers using end-to-end networks usually perform better than the trackers merely using feature extraction networks.(5)For visual tracking,the most suitable network training method is to per-train networks with video information and online fine-tune them with subsequent observations.Finally,we summarize our manuscript and highlight our insights,and point out the further trends for deep visual tracking. | Yongxiang Gu Beijing Chen Xu Cheng Yifeng Zhang Jingang Shi | 2019 | Journal of Information Hiding and Privacy Protection2019，1，1： | 0 |
| 19 | Transferrin-guided intelligent nanovesicles augment the targetability and potency of clinical PLK1 inhibitor to acute myeloid leukemia显示文摘Acute myeloid leukemia(AML)remains a most lethal hematological malignancy,partly because of its slow development of targeted therapies compared with other cancers.PLK1 inhibitor,volasertib(Vol),is among the few molecular targeted drugs granted breakthrough therapy status for AML;however,its fast clearance and dose-limiting toxicity greatly restrain its clinical benefits.Here,we report that transferrin-guided polymersomes(TPs)markedly augment the targetability,potency and safety of Vol to AML.Vol-loaded TPs(TPVol)with 4%trans-ferrin exhibited best cellular uptake,effective down-regulation of p-PLK1,p-PTEN and p-AKT and superior apoptotic activity to free Vol in MV-4-11 leukemic cells.Intravenous injection of TPVol gave 6-fold higher AUC than free Vol and notable accumulation in AML-residing bone marrow.The efficacy studies in orthotopic MV-4-11 leukemic model demonstrated that TPVol significantly reduced leukemic cell proportions in periphery blood,bone marrow,liver and spleen,effectively enhanced mouse survival rate,and impeded bone loss.This transferrin-guided nano-delivery of molecular targeted drugs appears to be an interesting strategy towards the development of novel treatments for AML. | Yifeng Xia Jingnan An Jiaying Li Wenxing Gu Yifan Zhang Songsong Zhao Cenzhu Zhao Yang Xu Bin Li Zhiyuan Zhong Fenghua Meng | 2023 | Bioactive Materials2023，，3： | 0 |
| 20 | Advances in the Treatment of Acute ITP显示文摘Immune thrombocytopenia(ITP),also known as idiopathic thrombocytopenic purpura,is a hematologic disease in which platelet destruction increases and production decreases,mainly mediated by immunity.However,up to now,the pathogenesis of ITP is not clear,which has caused great trouble in treatment.Therefore,this paper intends to review the recent literature on ITP treatment to provide some reference for clinical treatment.This paper combines the evidence of ASH immune thrombocytopenia and the Chinese Guidelines for the Diagnosis and Treatment of Adult primary immune thrombocytopenia(2020 edition).In this paper,the treatment process of ITP was divided into first-line treatment and second-line treatment,and it was concluded that first-line treatment was often used when newly diagnosed with ITP:combination of high-dose dexamethasone and low-dose gamma globulin.However,it should be noted that only 60%-80%of patients responded to first-line treatment,and most of them relapsed during dosing reduction or drug withdrawal.Second-line treatment is recommended in this case.The standard treatment is splenectomy,but due to its uncontrollable nature and side effects,it should be used cautiously.A new treatment approach,thrombogenin receptor agonist,has great development potential,and multidrug combination therapy for ITP has positive clinical significance. | Yuheng Jiang Jiahao Gu Zai Gu Yifeng Xu Bin Jia Jing Yang | 2022 | Journal of Integrative Medicine(双语)2022，11，1： | 0 |
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