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| 1 | The OsAMT1.1 gene functions in ammonium uptake and ammoniumepotassium homeostasis over low and high ammonium concentration ranges显示文摘Rice(Oryza sativa) grown in paddy fields is an ammonium(NH^+_4)-preferring crop;however,its AMT-type NH^+_4transporters that mediate root N acquisition have not been well characterized yet.In this study,we analyzed the expression pattern and physiological function of the OsAMT1.1 gene of the AMT1 subfamily in rice.Os AMT1.1 is located in the plasma membrane and is mainly expressed in the root epidermis,stele and mesophyll cells.Disruption of the Os AMT1.1 gene decreased the uptake of NH^+_4,and the growth of roots and shoots under both low NH^+_4and high NH^+_4conditions.Os AMT1.1 contributed to the short-term(5 min)^(15)NH^+_4influx rate by approximately one-quarter,irrespective of the NH^+_4concentration.Knockout of Os AMT1.1 significantly decreased the total N transport from roots to shoots under low NH^+_4conditions.Moreover,compared with the wild type,the osamt1.1 mutant showed an increase in the potassium(K)absorption rate under high NH^+_4conditions and a decrease under low NH^+_4conditions.The mutants contained a significantly high concentration of K in both the roots and shoots at a limited K(0.1 mmol/L)supply when NH^+_4was replete.Taken together,the results indicated that OsAMT1.1 significantly contributes to the NH^+_4uptake under both low and high NH^+_4conditions and plays an important role in Ne K homeostasis in rice. | Chang Li Zhong Tang Jia Wei Hongye Qu Yanjie Xie Guohua Xu | 2016 | Journal of Genetics and Genomics2016，43，11： | 12 |
| 2 | Effects of dietary leucine supplementation on the gene expression of mammalian target of rapamycin signaling pathway and intestinal development of broilers显示文摘This experiment was to investigate the effects of dietary leucine supplementation on the gene expression of mammalian target of rapamycin(mTOR) signaling pathway and intestinal development of broilers. A total of 384 one-day-old broilers were randomly assigned into 4 treatments with 6 replicates(16 broilers per replicate). Broilers in these treatment groups were offered the following diets with 1.37,1.77,2.17 and2.57% of leucine. These diet treatments were named 1.37 TM, 1.77 TM, 2.17 TM, and 2.57 TM. The experiment lasted 21 days and all birds had free access to feed and water. Results indicated that there was no significant difference in body weight, average daily gain and average feed intake among all treatments(P > 0.05). The broiler duodenal villus height in 2.57 TM was the lowest, but the highest occurred in1.37 TM on d 7 and 14(P < 0.05). The villus height in the jejunum and ileum increased along with leucine level from 1.37 to 2.17%. The villus height of jejunum was significantly higher in 2.17 TM than in 1.37 TM on d 7 and 14, and the ratio of villus height to crypt depth(V:C) in the duodenum, jejunum and ileum increased significantly(P < 0.05) on d 21. The gene expression level of mTOR in the duodenum decreased with increasing leucine level and was higher in 1.37 TM than in 2.57 TM on d 7 and 14(P < 0.05). On d 14 and 21 of the trial, the expression of S6 K1 in the duodenum was higher in 1.37 TM than in 2.57 TM(P < 0.05), and the expression of mTOR, S6 K1 in the jejunum and ileum increased with increasing leucine level form 1.37 to 2.17%, whereas a significant difference occurred between 1.37 TM and 2.17 TM(P< 0.05).In conclusion, the addition of leucine fails to enhance the growth performance of broilers. However,leucine can improve intestinal development by enhancing villus height and V:C ratio in the jejunum and ileum. Moreover, the expression of mTOR, S6 K1 increased as the level of dietary leucine was elevated from 1.37 to 2.17%. | Yinlian Chang Huiyi Cai Guohua Liu Wenhuan Chang Aijuan Zheng Shu Zhang Ruibo Liao Wei Liu Yang Li Jia Tian | 2015 | Animal Nutrition2015，，4： | 6 |
| 3 | Molecular mechanisms of growth depression in broiler chickens(Gallus Gallus domesticus)mediated by immune stress:a hepatic proteome study显示文摘Background:Immunological stress decreases feed intake,suppresses growth and induces economic losses.However,the underlying molecular mechanism remains unclear.Label-free liquid chromatography and mass spectrometry(LC-MS)proteomics techniques were employed to investigate effects of immune stress on the hepatic proteome changes of Arbor Acres broilers(Gallus Gallus domesticus)challenged with Escherichia coli lipopolysaccharide(LPS).Results:Proteomic analysis indicated that 111 proteins were differentially expressed in the liver of broiler chickens from the immune stress group.Of these,28 proteins were down-regulated,and 83 proteins were up-regulated in the immune stress group.Enrichment analysis showed that immune stress upregulated the expression of hepatic proteins involved in defense function,amino acid catabolism,ion transport,wound healing,and hormone secretion.Furthermore,immune stress increased valine,leucine and isoleucine degradation pathways.Conclusion:The data suggests that growth depression of broiler chickens induced by immune stress is triggered by hepatic proteome alterations,and provides a new insight into the mechanism by which immune challenge impairs poultry production. | Aijuan Zheng Anrong Zhang Zhimin Chen Shoaib Ahmed Pirzado Wenhuan Chang Huiyi Cai Wayne L.Bryden Guohua Liu | 2021 | Journal of Animal Science and Biotechnology2021，12，4： | 4 |
| 4 | Dual inhibition of EGFR at protein and activity level via combinatorial blocking of PI4KIla as anti-tumor strategy显示文摘 | Jiangmei Li Lunfeng Zhang Zhen Gao Hua Kang Guohua Rong Xu Zhang Chang Chen | 2014 | Protein & Cell2014，5，6： | 2 |
| 5 | Effects on antioxidant enzyme activities and osmolytes in Halocnemum strobilaceum under salt stress显示文摘The seedlings of Halocnermum strobilaceum were cultivated in 0.5%hoagland nutrient solution containing 0.0%,0.9%,2.7%and 5.4%of NaCl as well as composite salt(Na^+,Ca^(2+),K^+,Si^(4+)) for 20 days;all the contents are in weight ratio.Succulent level,inorganic ions(Na^+,K^+),organics such as betaine,proline,malondialdehyde,and antioxidant enzyme activities including superoxide dismutase(SOD),catalase(CAT),peroxidase(POD),betaine aldehyde dehydrogenase(BADH) were measured to reveal its salt tolerance mechanism.When the composite salt concentration reaches 5.4%,SOD activity level,and MDA content is five times the control group;when it reaches 2.7%,the succulent level of seedlings,and the content of K^+ in roots is nearly two times the NaCl treatment;the dry weight is more than three times the control group;with the NaCl treatment,MDA is three times the contrast;when the salt concentration is 2.7%,POD reaches the maximum.Results indicate that Si^(4+),K^+,and Ca^(2+) from composite salt in the roots of H.strobilaceum improved the water-holding capacity.The activities of antioxidant enzyme were raised by the accumulation of proline and betaine,which increased the salt tolerance.The absorption of K^+ promoted the high ratio of K^+/Na^+ and alleviated the damage of cell membranes of H.strobilaceum,which is associated with osmotic contents such as betaine and proline. | TianPeng Gao Rui Guo XiangWen Fang ZhiGang Zhao GuoHua Chang YinQuan Chen Qing Zhang | 2016 | Research in Cold and Arid Regions2016，8，1： | 2 |
| 6 | Kinetic Spectmpho-tome~c Determination of Tmoe Manganese ( !] ) with Dahlia Violet inNonionic Micmenmlslon Medium 显示文摘 | Wei Qin Yah Liangguo Chang Guohua et 81 | 2003 | Talanta2003，59，2： | 1 |
| 7 | Talanta显示文摘 | Wei Qin Yan Liangguo Chang Guohua Ou Qingyu | 2003 | 59(2):2532003，59，2： | 1 |
| 8 | Using high impedance ground plane for radiation improving in microwave antennas显示文摘 | Zhang Guohua Zhu Chang Fu Yunqi | 2005 | IEEE APMC2005 Proceedings2005，11，： | 1 |
| 9 | apllications of factorcriteria system reconstruction analysis in the reproduction research on grass carp, black carp, silver carp and bighead in the Yangtze River显示文摘 | Zhang Guohua Chang Jianbo Shu Guangfu | 1998 | Int J General System1998，29，3： | 1 |
| 10 | IGFBP-rP1 induces p21 expression through a p53-independent pathway, leading to cellular senescence of MCF-7 breast cancer cells显示文摘 | Shuguang Zuo Chang Liu Jianguo Wang Fuqing Wang Wanling Xu Shao Cui Lei Yuan Xudong Chen Wenjuan Fan Mingchen Cui Guohua Song | 2012 | Journal of Cancer Research and Clinical Oncology2012，，6： | 1 |
| 11 | The influence of seeding conditions and shielding gas atmosphere on the synthesis of silver nanowires through the polyol process显示文摘 | Chen Chang Wang Li Jiang Guohua | 2006 | Nanotechnology2006，17，2： | 1 |
| 12 | Research on Fault Diagnosis Expert System Based on the Neural Network and the Fault Tree Technology显示文摘 | Yingying Wang Qiuju Li Ming Chang Hongwei Chen Guohua Zang | 2012 | Procedia Engineering2012，，： | 1 |
| 13 | Apllications of factorcriteria system reconstruction analysis in the reproduction research on grass carp,black carp,silver carp and bighead in the Yangtze River显示文摘 | Zhang Guohua Chang Jianbo Shu Guangfu | | 0，，03： | 1 |
| 14 | Talanta显示文摘 | Wei Qin Yang Jinhu Zhang Yong Chang Guohua Du Bin | 2002 | 58(3):4192002，58，3： | 1 |
| 15 | Theeffects of afforestation on soil organic and inorganic car-bon: A case study of the Loess Plateau of China显示文摘 | Chang Ruiying Fu Bojie Liu Guohua | 2012 | Catena2012，，95： | 1 |
| 16 | Analysis and Simulation on Current Commutation of DC Transfer Switches in UHVDC Transmission Systems显示文摘 | PENG Chang WEN Jialiang MA Guohua WANG Xiuhuan LIU Zhigang YU Kunshan | 2011 | 中国电机工程学报2011，31，36： | 1 |
| 17 | Sea level change along the coast of China relative to the isostatic datum显示文摘On the basis of the analyses of significant periods for the sea level observation data taken from recent several decades at 12 tide stations, the monthly mean sea level observations are fitted by a model of linear trend of sea level change superimposed with several variations of different fixed periods. The trends of sea level relative changes and their errors are estimated by the LS method. The results are reduced to the isostatic datum proposed and established in the paper (Huang et al. , 1991, Seismology and Geology , 1, 1-15). The trends of sea level changes in the near future along the coast of China are studied. It is pointed out that the general trend of the sea level change along the coast of China is going up slowly and the rate of the change is not the same in different segments of the coasts. In a few segments, the sea level is even relatively going down. The numerical results given in this paper provide a basis for the predictions of the future sea level changes and their effects. | Huang Liren, Yang Guohua An Zhensheng Geodetic Brigade, State Seismological Bureau, Tianjin 300180, ChinaInstitute of Marine Scientific and Technological Information, State Oceanic Administration, Tianjin 300171, China Xie Zhiren, A brief discussion on the trend of sea level change and prediction, 1989. | 1991 | Acta Oceanologica Sinica1991，10，4： | 0 |
| 18 | Effects of dynamic segmentation of nutrient supply on growth performance and intestinal development of broilers显示文摘This experiment was to investigate the effects of dynamic segmentation of interval nutrient supply phase feeding on the growth performance, carcass characteristics, immune organs indexes and intestinal morphology of broilers. A total of 320 one-day-old broilers were randomly assigned into 4 feeding treatments, which included 4 interval nutrient supply phases as follows. Treatment A: a nutrient supply standard was used for every 14 d. Treatment B: a nutrient supply standard was used for every 7 d. Treatment C: a nutrient supply standard was used for every 3.5 d except that one nutrient supply standard was used for d 1 to 7. Treatment D: a nutrient supply standard was used for every 3.5 d including d 1 to 7. Each treatment was represented by 8 replicates with 10 broilers per replicate. The trial lasted for 42 days. Throughout the 42 d trial period, treatment A showed significantly higher average daily gain than treatments B and C(P < 0.05). The feed:gain ratio of treatment A was significantly lower than those of treatments C and D(P < 0.05). On d 28 and 42, body weight of broilers in treatment A was much higher than those of treatments B and C(P < 0.05). The slaughter rate of treatment A was significantly higher than that of treatment B(P < 0.05). Eviscerated percentage of treatment A was significantly higher than those of treatment B and D(P < 0.05). There were no significant differences among 4 treatments in immune organs indexes(P > 0.05). The crypt depth of duodenum was significantly greater in treatments A and B than in treatment C on day 42(P < 0.05).Meanwhile, the ratio of villus height to crypt depth(V:C ratio) of treatment D was significantly higher than that of treatment A(P < 0.05). No significant differences were found between treatments C and D in growth performance, carcass performance, immune organs indexes and intestinal structure(P> 0.05). In conclusion, this study revealed that the growth and carcass performance of broilers is the best for 14 days segmentation phase feeding, and 3.5 days segmentation interval phase feeding can promote small intestinal development of broilers. Dynamic segmentation of dietary supply fails to affect the immune function of broilers. | Xiangyang Huang Huiyi Cai Guohua Liu Haijie Yan Wenhuan Chang Shu Zhang | 2016 | Animal Nutrition2016，，4： | 0 |
| 19 | Terahertz switch and polarization controller based on photonic crystal fiber显示文摘Theoretical studies on liquid crystal filled photonic crystal fiber(LC-PCF)are presented.The eects of electric birefringence of liquid crystal(LC)in the LC-PCF and the transmitting properties of photonic crystal fiber(PCF)are investigated by using the full vector plane wave expansion and beam propagation method.The simulation results show that the electrically controlled LC-PCF can act as not only a terahertz(THz)switch with about 0.55 THz bandwidth,but also a tunable polarization controller for changing the polarization state of the fundamental mode. | HOU Yu FAN Fei WANG XiangHui ZHAO GuoHua CHANG ShengJiang | 2012 | Science China(Information Sciences)2012，55，1： | 0 |
| 20 | From the UN Charter and the Universal Declaration of Human Rights to Building a Community with a Shared Future for Human Beings--The Past, Present, and Future of International Human Rights Protection显示文摘The UN Charter first of all affirmed the basic concept of international protection of human rights and opened up a broad space for international protection of human rights. The Universal Declaration of Human Rights is an important document in the development of the international human rights cause. It established a set of international standards for basic human rights, represents the world's most important consensus on human rights, and contributes greatly to the creation of an international human rights legal system. The important theory put forward by President Xi Jinping to build a Community with a Shared Future for Human Beings, as a Chinese program for today s global major issues, also outlines a new blueprint for national human rights protection and will play a major role in promoting the development of China's human rights and international human rights protection. | 刘海年 CHANG Guohua(译) | 2018 | The Journal of Human Rights2018，17，5： | 0 |
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