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| 1 | Removal of Crystal Violet from aqueous solution using powdered mycelial biomass of Ceriporia lacerata P2显示文摘A biosorbent prepared from powdered mycelial biomass of Ceriporia lacerata (CLB), a basidiomycetous fungus, was applied for the uptake of Crystal Violet from aqueous solution. A batch adsorption experiment was used for the biosorption process, involving effect of experimental factors and biosorption kinetics and equilibrium. Biosorption process showed that the removal of Crystal Violet by CLB was effective over wide pH range, and meanwhile was independent on ionic strength. Biosorption capacities of CLB increased with the initial dye concentration increasing, due to an increase in the driving force of the concentration gradient. The adsorbed Crystal Violet amount per unit biomass weight decreased with increasing biosorbent dosage, due to the splitting effect of flux (concentration gradient) between sorbate and biosorbent. A maximum sorption capacity of 239.25 mg/g was observed. Biosorption kinetics was found to be best represented by the pseudo second-order kinetic model. The equilibrium adsorption data was well described by the Koble-Corrigan model. FT-IR (Fourier Transform Infrared Spetroscopy) spectrum showed the presence of O-H, COOH, C=O, C-N, C-H, -NH2 and P-OH in the surface of CLB as functional groups. This study showed CLB can effectively remove CV from dye wastewater. | Yonghui Lin Xingbing He Guomin Han Qijian Tian Wenyong Hu | 2011 | Journal of Environmental Sciences2011，23，12： | 11 |
| 2 | Three-dimensional cell culture models for investigating human viruses显示文摘Three-dimensional(3D) culture models are physiologically relevant, as they provide reproducible results, experimental flexibility and can be adapted for high-throughput experiments. Moreover,these models bridge the gap between traditional two-dimensional(2D) monolayer cultures and animal models. 3D culture systems have significantly advanced basic cell science and tissue engineering, especially in the fields of cell biology and physiology, stem cell research, regenerative medicine, cancer research, drug discovery, and gene and protein expression studies. In addition,3D models can provide unique insight into bacteriology, virology, parasitology and host-pathogen interactions. This review summarizes and analyzes recent progress in human virological research with 3D cell culture models. We discuss viral growth, replication, proliferation, infection, virus-host interactions and antiviral drugs in 3D culture models. | Bing He Guomin Chen Yi Zeng | 2016 | Virologica Sinica2016，31，5： | 5 |
| 3 | SNP-based QTL mapping for panicle traits in the japonica super rice cultivar Liaoxing 1显示文摘Panicle traits directly associated with yield are a target of selection in rice breeding.Although abundant QTL for panicle traits have been identified,there is little information about the genetic basis of panicle traits in japonica super rice(JSR)cultivars.In this study,we identified QTL for panicle traits in three environments using a population of recombinant inbred lines(RILs)derived from the JSR cultivar Liaoxing 1.A total of 197 RILs were genotyped with 285 polymorphic SNP markers.Phenotypic data and best linear unbiased prediction(BLUP)value of primary branch number(BNP),secondary branch number(BNS),grain number on primary branch(GNP),grain number on secondary branch(GNS),grain number per panicle(GN),panicle length(PL)and grain density(GD)were used for QTL mapping.A total of 105 QTL for seven panicle traits were detected in single environments using their BLUP values.Individual QTL explained 0.51%–52.22%of the phenotypic variation.Of the 105,49 were also detected by joint multi-environment analyses.Five stable QTL:qGD9,qPL9,qGNP9,qGN6,and qBNS6.2 were identified in multiple environments.qGD9,qGNP9,and qPL9,co-localizing on chromosome 9,likely correspond to the known gene DEP1.Importantly,qGN6 and qBNS6.2 in a co-localization region were identified as novel QTL,and their Liaoxing 1 alleles had a positive effect.Several RILs with the QTL allele combinations qGD9/qPL9/qGNP9 and qGN6/qBNS6.2 showed greater GN.Further investigation of the putative gene underlying qGN6/qBNS6.2 would shed light on the molecular mechanism of JSR. | Mingzhu Zhao Zuobin Ma Lili Wang Zhiqiang Tang Ting Mao Chuanbin Liang Hong Gao Liying Zhang Na He Liang Fu Changhua Wang Guomin Sui Wenjing Zheng | 2020 | The Crop Journal2020，8，5： | 4 |
| 4 | Artful Application of Garden Plants in Classical Suzhou Gardens显示文摘Defi nition of plant landscaping and features of plant landscaping in classical Suzhou gardens were introduced, the forms of combining plants and buildings, rockeries and waterscapes were analyzed by elaborating selection of plant species and plant furnishings. In addition, manifestation of artistic beauty of the classical Suzhou gardens was explored, and protective measures were given. | XU Xifeng HE Guomin | 2014 | Journal of Landscape Research2014，6，1： | 2 |
| 5 | A Principle of Stream Arrangement Based on Uniformity Factor for Heat Exchanger Networks Synthesis显示文摘 | He Qiaole Cui Guomin | | 0，，2： | 1 |
| 6 | Evaluation of a new fungus Ceriporia lacerate strain P2—its ability to decolorize Alizarin Red and Methyl Orange显示文摘 | Liang Xu Yue Zhu Xingbing He Guomin Han Xingjun Tian | | 0，2008，： | 1 |
| 7 | Transcriptome-guided identification of a four-component system, SbrH1-R, that modulates milbemycin biosynthesis by influencing gene cluster expression, precursor supply, and antibiotic efflux显示文摘Streptomyces can produce numerous antibiotics and many other bioactive compounds.Recently,the molecular mechanisms of transcriptional regulators in control of antibiotic production by influencing the expression of biosynthetic gene clusters(BGCs)have been extensively studied.However,for regulators that affect both antibiotic production and cell growth,the way to influence antibiotic production may be diverse,but related studies are limited.Here,based on time-course transcriptome analysis,a four-component system,SbrH1-R,consisting of the two-component system SbrKR(SBI_03479/3478)and two hypothetical proteins SbrH1(SBI_03481)and SbrH2(SBI_03480)potentially related with the biosynthesis of milbemycins was identified in Streptomyces bingchenggensis BC-101-4.Deletion of sbrH1-R resulted in weakened cell growth but a 110%increase of milbemycin production compared with that in BC-101-4.Comparative transcriptome analyses of the sbrH1-R mutant and BC-101-4 revealed that SbrH1-R not only indirectly represses milbemycin BGC expression,but also inhibits milbemycin production by modulating expression levels of genes related to precursor supply and antibiotic efflux.Further genetic experiments identified several new targets,including five precursor supply-associated reactions/pathways(e.g.,the reaction from pyruvate to acetyl-CoA,the reaction from acetyl-CoA to citrate,the fatty acidβ-oxidation process,and the branched chain amino acid and phenylalanine acid degradation pathways)and a milbemycin exporter system(MilEX2)that can be engineered for milbemycin overproduction.These results shed new light on the understanding of regulation of milbemycin biosynthesis and provide useful targets for future metabolic engineering of the native host to improve milbemycin production. | Lan Ye Yanyan Zhang Shanshan Li Hairong He Guomin Ai Xiangjing Wang Wensheng Xiang | 2022 | Synthetic and Systems Biotechnology2022，7，2： | 1 |
| 8 | Analysis of parental genetic diversity and its impact on grain yield and quality of japonica hybrid rice in northern China显示文摘The relationship between parental genetic differences and the quality and yield of japonica hybrid rice strongly influences japonica hybrid rice breeding.In this study,137 parental lines of japonica hybrid rice were genotyped using 8 K rice SNP-Chips to characterize their genetic diversity,population structure,and indica-genotype proportion.The genetic diversity of total parental lines averaged 0.264,with values of0.287 for restorer lines and 0.148 for the sterile lines.The introduction of indica lineage increased the genetic diversity of restorer lines relative to that of sterile lines.By model-based population structure analysis,the 137 lines were divided into 14 groups.According to the grouping results,eight restorer lines and five sterile lines were selected from different groups for cross breeding,yielding 40 japonica hybrid rice combinations(F1).Investigation of the yield and quality of these combinations showed that highyield combinations could be obtained by increasing parental genetic distance to 0.8–0.9,a result accomplished largely by the introduction of indica genomic components of restorer lines.To further improve grain quality,the genetic distance between parents should be reduced to 0.4–0.5,suggesting an indicagenotype proportion of 30%–40%for restorer lines.This study may provide a reference for breeding of japonica hybrid rice. | Hong Gao Zuobin Ma Yuanzheng Wang Manli Zhang Xianju Wang Changhua Wang Zhiqiang Tang Liying Zhang Liang Fu Na He Hui Wang Yongan Yin Yuanjun Bai Guomin Sui Wenjing Zheng | 2022 | The Crop Journal2022，10，3： | 1 |
| 9 | Preparation and in vitro release studies of thymosin-loaded PLA microspheres显示文摘 | He Yi Xiao Guomin | 2007 | J Southeast Univ2007，23，2： | 1 |
| 10 | Genetic Architecture of Childhood Kidney and Urological Diseases in China显示文摘Kidney disease is manifested in a wide variety of phenotypes,many of which have an important hereditary component.To delineate the genotypic and phenotypic spectrum of pediatric nephropathy,a multicenter registration system is being imple-mented based on the Chinese Children Genetic Kidney Disease Database(CCGKDD).In this study,all the patients with kidney and urological diseases were recruited from 2014 to 2020.Genetic analysis was conducted using exome sequencing for families with multiple affected individuals with nephropathy or clinical suspicion of a genetic kidney disease owing to early-onset or extrarenal features.The genetic diagnosis was confirmed in 883 of 2256(39.1%)patients from 23 provinces in China.Phenotypic profiles showed that the primary diagnosis included steroid-resistant nephrotic syndrome(SRNS,23.5%),glomerulonephritis(GN,32.2%),congenital anomalies of the kidney and urinary tract(CAKUT,21.2%),cystic renal disease(3.9%),renal calcinosis/stone(3.6%),tubulopathy(9.7%),and chronic kidney disease of unknown etiology(CKDu,5.8%).The pathogenic variants of 105 monogenetic disorders were identified.Ten distinct genomic disorders were identified as pathogenic copy number variants(CNVs)in 11 patients.The diagnostic yield differed by subgroups,and was highest in those with cystic renal disease(66.3%),followed by tubulopathy(58.4%),GN(57.7%),CKDu(43.5%),SRNS(29.2%),renal calcinosis/stone(29.3%)and CAKUT(8.6%).Reverse phenotyping permitted correct identification in 40 cases with clinical reassessment and unexpected genetic conditions.We present the results of the largest cohort of children with kidney disease in China where diagnostic exome sequencing was performed.Our data demonstrate the utility of family-based exome sequencing,and indicate that the combined analysis of genotype and phenotype based on the national patient registry is pivotal to the genetic diagnosis of kidney disease. | Ye Fang Hua Shi Tianchao Xiang Jiaojiao Liu Jialu Liu Xiaoshan Tang Xiaoyan Fang Jing Chen Yihui Zhai Qian Shen Guomin Li Li Sun Yunli Bi Xiang Wang Yanyan Qian Bingbing Wu Huijun Wang Wenhao Zhou Duan Ma Jianhua Mao Xiaoyun Jiang Shuzhen Sun Ying Shen Xiaorong Liu Aihua Zhang Xiaowen Wang Wenyan Huang Qiu Li Mo Wang Xiaojie Gao Yubin Wu Fang Deng Ruifeng Zhang Cuihua Liu Li Yu Jieqiu Zhuang Qing Sun Xiqiang Dang Haitao Bai Ying Zhu Siguang Lu Bili Zhang Xiaoshan Shao Xuemei Liu Mei Han Lijun Zhao Yuling Liu Jian Gao Ying Bao Dongfeng Zhang Qingshan Ma Liping Zhao Zhengkun Xia Biao Lu Yulong Wang Mengzhun Zhao Jianjiang Zhang Shan Jian Guohua He Huifeng Zhang Bo Zhao Xiaohua LI Feiyan Wang Yufeng Li Hongtao Zhu Xinhui Luo Jinghai Li Jia Rao Hong Xu | 2021 | Phenomics2021，1，3： | 0 |
| 11 | Isolation and Identification of Bacterial Pathogens of Proteus vulgaris and Chryseobacterium meningosepticum from Peodiscus sinensis and Observation on the Histopathology显示文摘Two dominant strains of bacteria Lb18-01 and Lb18-02 were isolated and purified from scabies,liver and intestine of diseased Peodiscus sinensis.By artificial infection test,the two strains were verified as pathogenic strains with similar characters to that of natural infectious cases.The strains Lb18-01 and Lb18-02 showed strong pathogenicity to healthy P.sinensis in the artificial infection experiment,so they were the pathogenic strain of the disease.According to the morphology,physicochemical characteristics,16 S rDNA sequence analysis and phylogenetic tree clustering,the pathogenic strains Lb18-01 and Lb18-02 were identified as Proteus vulgaris and Chryseobacterium meningosepticum which were tolerant to 20 drugs such as penicillin,tetracycline and ampicillin.Histopathological observation on diseased P.sinensis showed the pathological symptoms of sepsis such as hemorrhage and congestion of liver,spleen and intestine,and glomerular disintegration. | Guomin JIANG Li LIU Wuying CHU Zhigang HE Li ZOU Dongwu WANG Shiming DENG | 2021 | Agricultural Biotechnology2021，10，2： | 0 |
| 12 | Analysis and Verification of the Conserved MYB Binding Element in the DFR Promoter in Compositae显示文摘Anthocyanins,ubiquitous in the Compositae family,are regulated by MYB(v-myb avian myeloblastosis viral oncogene homolog),playing an important role in anthocyanin synthesis.In this study,we analyzed the regulation pathway in which the MYB protein of subgroup 6 promotes dihydroflavonol reductase(DFR)expression in Compositae,and validated this law in Saussurea medusa through yeast one-hybrid experiments.Our results showed that MYB and DFR underwent purification selection,DFR promoter analysis revealed the presence of MYB binding site(GAGTTGAATGG)and bHLH binding site(CANNTG)at the sense strand of 84–116 nucleotide residues from the start codon.These two motifs were separated by 9–10 nucleotide residues,as existed in the DFR promoters of many Compositae plants.Furthermore,the yeast one-hybrid experiment demonstrated that SmMYB1 can activate the promoter of SmDFR.Our results provide a reference for further functional study of DFR in Compositae. | Jialei Guo Fengzhen Li Guomin Shi Weimin Zhao Tao He | 2024 | Phyton-International Journal of Experimental Botany2024，93，2： | 0 |
| 13 | Comparative Transcriptome Analysis Reveals Different Mechanisms of Adaptation to Environment among Three Species of Saussurea DC.显示文摘Saussurea medusa,Saussurea hypsipeta and Saussurea obvallata are typical alpine snowline plants growing in the Qinghai-Tibet plateau.They are characterized by a specialized morphology.S.medusa and S.hypsipeta have very dense trichomes on whole plant,whereas S.obvallata has transparent bracts covered inflorescences.The different forms reflect their adaptation to cold environments.To investigate the different mechanisms of adaptation of these species to cold temperatures,transcriptome sequencing was performed in three species of Saussurea DC.A total of 116394137237 and 113879 Unigenes were identified from S.medusa,S.hypsipeta and S.obvallata,respectively.Of these,55909(48.03%),65519(47.74%)and 51889(45.56%)Unigenes were matched in public databases.GO analysis identified that most of annotated Unigenes in the three species of plants were related to cellular,metabolic,and single−organism processes,and binding and catalytic activities.The differential expression of 37 genes related to environmental adaptation were discovered by pairwise comparisons.Of these,two candidate genes(Interaptin-like and CSLB3)related to trichome development were identified only in S.medusa and S.hypsipeta,which was consistent with their distinct morphology.Our data can provide a valuable resource for the further studies on the adaptive mechanisms of molecular and functional ecology in Saussurea DC. | Weimin Zhao Fengzhen Li Guomin Shi Jialei Guo Guifang He Tao He | 2022 | Phyton-International Journal of Experimental Botany2022，91，7： | 0 |
| 14 | NERank+: a graph-based approach for entity ranking in document collections显示文摘 | Chengyu WANG Guomin ZHOU Xiaofeng HE Aoying ZHOU | 2018 | Frontiers of Computer Science2018，12，3： | 0 |
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