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| 1 | Injectable hydrogels for cartilage and bone tissue engineering显示文摘Tissue engineering has become a promising strategy for repairing damaged cartilage and bone tissue. Among the scaffolds for tissue-engineering applications, injectable hydrogels have demonstrated great potential for use as three-dimensional cell culture scaffolds in cartilage and bone tissue engineering, owing to their high water content, similarity to the natural extracellular matrix(ECM), porous framework for cell transplantation and proliferation, minimal invasive properties, and ability to match irregular defects. In this review, we describe the selection of appropriate biomaterials and fabrication methods to prepare novel injectable hydrogels for cartilage and bone tissue engineering. In addition, the biology of cartilage and the bony ECM is also summarized. Finally, future perspectives for injectable hydrogels in cartilage and bone tissue engineering are discussed. | Mei Liu Xin Zeng Chao Ma Huan Yi Zeeshan Ali Xianbo Mou Song Li Yan Deng Nongyue He | 2017 | Bone Research2017，5，2： | 54 |
| 2 | Gd-EOB-DTPA-enhanced magnetic resonance imaging for focal liver lesions in Chinese patients:a multicenter,open-label,phase Ⅲ study显示文摘BACKGROUND:Contrast agents help to improve visibility in magnetic resonance(MR)imaging.However,owing to the large interstitial spaces of the liver,there is a reduction in the natural contrast gradient between lesions and healthy tissue.This study was undertaken to evaluate the efficacy and safety of the liverspecific MR imaging contrast agent gadoxetate disodium(GdEOB-DTPA)in Chinese patients.METHODS:This was a single-arm,open-label,multicenter study in patients with known or suspected focal liver lesions referred for contrast-enhanced MR imaging.MR imaging was performed in 234 patients before and after a single intravenous bolus of Gd-EOB-DTPA(0.025 mmol/kg body weight).Images were evaluated by clinical study investigators and three independent,blinded radiologists.The primary efficacy endpoint was sensitivity in lesion detection.RESULTS:Gd-EOB-DTPA improved sensitivity in lesion detection by 9.46%compared with pre-contrast imaging for the average of the three blinded readers(94.78%vs 85.32%for Gd-EOB-DTPA vs pre-contrast,respectively).Improvements in detection were more pronounced in lesions less than 1cm.Gd-EOB-DTPA improved diagnostic accuracy in lesion classification.CONCLUSIONS:This open-label study demonstrated that Gd-EOB-DTPA improves diagnostic sensitivity in liver lesions,particularly in those smaller than 1 cm.Gd-EOB-DTPA also significantly improves the diagnostic accuracy in lesion classification,and furthermore,Gd-EOB-DTPA is safe in Chinese patients with liver lesions. | Meng-Su Zeng Hui-Yi Ye Liang Guo Wei-Jun Peng Jian-Ping Lu Gao-Jun Teng Josy Breuer Yi Huan Ping Li Jian-Rong Xu Chang-Hong Liang | 2013 | Hepatobiliary & Pancreatic Diseases International2013，12，6： | 31 |
| 3 | Recent advances in nano scaffolds for bone repair显示文摘Biomedical applications of nanomaterials are exponentially increasing every year due to analogy to various cell receptors, ligands, structural proteins, and genetic materials(that is, DNA). In bone tissue, nanoscale materials can provide scaffold for excellent tissue repair via mechanical stimulation, releasing of various loaded drugs and mediators, 3D scaffold for cell growth and differentiation of bone marrow stem cells to osteocytes. This review will therefore highlight recent advancements on tissue and nanoscale materials interaction. | Huan Yi Fawad Ur Rehman Chunqiu Zhao Bin Liu Nongyue He | 2016 | Bone Research2016，4，4： | 17 |
| 4 | Electroacupuncture activates enteric glial cells and protects the gut barrier in hemorrhaged rats显示文摘AIM:To investigate whether electroacupuncture ST36 activates enteric glial cells,and alleviates gut inflammation and barrier dysfunction following hemorrhagic shock.METHODS:Sprague-Dawley rats were subjected to approximately 45% total blood loss and randomly divided into seven groups:(1) sham:cannulation,but no hemorrhage;(2) subjected to hemorrhagic shock(HS);(3) electroacupuncture(EA) ST36 after hemorrhage;(4) vagotomy(VGX)/EA:VGX before hemorrhage,then EA ST36;(5) VGX:VGX before hemorrhage;(6) a-bungarotoxin(BGT)/EA:intraperitoneal injection of a-BGT before hemorrhage,then EA ST36; and(7) a-BGT group:a-BGT injection before hemorrhage.Morphological changes in enteric glial cells(EGCs) were observed by immunofluorescence,and glial fibrillary acidic protein(GFAP; a protein marker of enteric glial activation) was evaluated using reverse transcriptase polymerase chain reaction and western blot analysis.Intestinal cytokine levels,gut permeability to 4-k Da fluorescein isothiocyanate(FITC)-dextran,and the expression and distribution of tight junction protein zona occludens(ZO)-1 were also determined.RESULTS:EGCs were distorted following hemorrhage and showed morphological abnormalities.EA ST36 attenuated the morphological changes in EGCs at 6 h,as compared with the VGX,a-BGT and HS groups.EA ST36 increased GFAP expression to a greater degree than in the other groups.EA ST36 decreased intestinal permeability to FITC-dextran(760.5 ± 96.43 ng/m L vs 2466.7 ± 131.60 ng/m L,P < 0.05) and preserved ZO-1 protein expression and localization at 6 h afterhemorrhage compared with the HS group.However,abdominal VGX and a-BGT treatment weakened or eliminated the effects of EA ST36.EA ST36 reduced tumor necrosis factor-a levels in intestinal homogenates after blood loss,while vagotomy or intraperitoneal injection of a-BGT before EA ST36 abolished its antiinflammatory effects.CONCLUSION:EA ST36 attenuates hemorrhageinduced intestinal inflammatory insult,and protects the intestinal barrier integrity,partly via activation of EGCs. | Sen Hu Zeng-Kai Zhao Rui Liu Hai-Bin Wang Chun-Yu Gu Hong-Min Luo Huan Wang Ming-Hua Du Yi Lv Xian Shi | 2015 | World Journal of Gastroenterology2015，21，5： | 15 |
| 5 | Anti-fibrotic Effects of Salvia miltiorrhiza and Ligustrazine Injection on LX-2 Cells Involved with Increased N-myc Downstream-Regulated Gene 2 Expression显示文摘Objective: To investigate the effects of Salvia miltiorrhiza and Ligustrazine Injection(SML) on proliferation and apoptosis of human hepatic stellate cell LX-2 and the expression of N-myc downstreamregulated gene 2(NDRG2, a tumor suppressor gene). Methods: HSCs from the LX-2 cell line were cultured in vitro. The proliferative state of different initial LX-2 cell numbers was measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) colorimetric assay. LX-2 cells were plated in 96-well plates at an approximate density of 2.50×10~4 cells/mL and cultured for 24 h followed by the application of different concentrations of SML(1, 2, 4 and 8 μL/mL). Cell proliferation was measured using the MTT assay at 24 and 48 h. Apoptosis was detected by flow cytometry at 24 h. LX-2 cells were treated with different concentrations of SML and extracted with protein lysis buffer. The levels of NDRG2 and β-catenin were measured by Western blot. Results: With the exception of the 1 and 2 μL/mL concentrations, 4 and 8 μL/mL SML inhibited cell proliferation in a concentration-dependent manner at 24 and 48 h(P<0.05). With the exception of the 1 and 2 μL/mL concentrations, the NDRG2 expression level was greatly increased in a concentration-dependent manner. However, the level of β-catenin was unaffected. Conclusion: SML inhibit LX-2 cell proliferation in a concentration-dependent manner, and the mechanism may be associated with NDRG2 over-expression. | zheng jin ma li-tian ren qin-you hu yue bai yang bian huan zhang yi zhou yong-chun yang ming-hui | 2017 | Chinese Journal of Integrative Medicine2017，23，12： | 13 |
| 6 | Roux-en-Y gastric bypass for Chinese type 2 diabetes mellitus patients with a BMI,28kg/m^2:a multi-institutional study显示文摘Roux-en-Y gastric bypass surgery(RYGB) has been demonstrated to be successful for treating type-II diabetes2mellitus(T2DM) patients with a body mass index(BMI),30 kg/m,but reports of RYGB for T2 DM patients with22 a BMI,28 kg/mare lacking.T2 DM patients with a BMI,28 kg/mwere prospectively recruited to participate in this study in four hospitals.The endpoint was T2 DM remission(defined by fasting blood glucose(FBG) level,110 mg/d L and hemoglobin(Hb)A1c level,6.0% at 12 months postoperatively).Predictors of remission were investigated by univariate and multivariate analyses.Eighty-six patients were assessed.Eighty-five patients underwent RYGB,with one conversion to open surgery.We compared the values of various variables before and after2 surgery.The mean BMI decreased from 24.68±2.12 to 21.72±2.43 kg/m(P,0.001).Fifty-eight(67.4%) patients were not treated by drugs or insulin after surgery,and 20 patients(23.3%) had complete remission of T2 DM at12 months after surgery with an acceptable number of complications.The mean Hb A1 c level in the remission group was significantly lower than that in the non-remission group.Patients with a higher weight,lower Hb A1 c level,higher C-peptide level,and higher FBG level were more likely to have T2 DM remission in multivariate2 analyses.In conclusion,RYGB was effective and safe for treating T2 DM patients with a BMI,28 kg/m.Complete remission can be predicted by cases having a higher weight,lower Hb A1 c level,higher C-peptide level,and higher FBG level. | Hui Liang Wei Guan Yanling Yang Zhongqi Mao Yijun Mei Huan Liu Yi Miao | 2015 | The Journal of Biomedical Research2015，29，2： | 13 |
| 7 | Anti-diabetic effects and mechanisms of action of a Chinese herbal medicine preparation JQ-R in vitro and in diabetic KKAy mice显示文摘Refined-JQ(JQ-R) is a mixture of refined extracts from Coptis chinensis(Ranunculaceae),Astragalus membranaceus(Leguminosae) and Lonicera japonica(Caprifoliaceae), the three major herbs of Jin Qi-Jiang Tang tablet, a traditional Chinese medicine(TCM) formula. The mechanisms by which JQ-R regulates glucose metabolism and improves insulin sensitivity were studied in type 2 diabetic KK^(Ay)mice and insulin-resistant L6 myotubes. To investigate the mechanisms by which JQ-R improves insulin sensitivity, a model of insulin-resistant cells induced with palmitic acid(PA) was established in L6 myotubes. Glucose uptake and expression of factors involved in insulin signaling, stress, and inflammatory pathways were detected by immunoblotting. JQ-R showed beneficial effects on glucose homeostasis and insulin resistance in a euglycemic clamp experiment and decreased fasting insulin levels in diabetic KK^(Ay)mice. JQ-R also improved the plasma lipid profiles. JQ-R directly increased the activity of superoxide dismutase(SOD) and decreased malondialdehyde(MDA) as well as inducible nitric oxide synthase(iNOS) levels in insulin-resistant L6 cells, and elevated the insulin-stimulated glucose uptake with upregulated phosphorylation of AKT. The phosphorylation levels of nuclear factor kappa B(NF-κB p65), inhibitor of NF-κB(IκBα), c-Jun N-terminal kinase(JNK1/2) and extracellular-signal-regulated kinases(ERK1/2) were also changed after JQ-R treatment compared with the control group. Together these findings suggest that JQ-R improved glucose and lipid metabolism in diabetic KK^(Ay)mice. JQ-R directly enhanced insulin-stimulated glucose uptake in insulin-resistant myotubes with improved insulin signalling and inflammatory response and oxidative stress. JQ-R could be a candidate to achieve improved glucose metabolism and insulin sensitivity in type 2 diabetes mellitus. | Quan Liu Shuainan Liu Lihui Gao Sujuan Sun Yi Huan Caina Li Yue Wang Nan Guo Zhufang Shen | 2017 | Acta Pharmaceutica Sinica B2017，7，4： | 11 |
| 8 | A miR-130a-YAP positive feedback loop promotes organ size and tumorigenesis显示文摘 | Shuying Shen Xiaocan Guo Huan Yan Yi Lu Xinyan Ji Li Li Tingbo Liang Dawang Zhou Xin-Hua Feng Jonathan C Zhao Jindan Yu Xing-Guo Gong Lei Zhang Bin Zhao | 2015 | Cell Research2015，25，9： | 10 |
| 9 | Low-Dimensional Halide Perovskites and Their Advanced Optoelectronic Applications显示文摘Metal halide perovskites are crystalline materials originally developed out of scientific curiosity. They have shown great potential as active materials in optoelectronic applications. In the last 6 years, their certified photovoltaic efficiencies have reached 22.1%. Compared to bulk halide perovskites, low-dimensional ones exhibited novel physical properties. The photoluminescence quantum yields of perovskite quantum dots are close to 100%. The external quantum efficiencies and current efficiencies of perovskite quantum dot light-emitting diodes have reached 8% and 43 cd A^(-1),respectively, and their nanowire lasers show ultralow-threshold room-temperature lasing with emission tunability and ease of synthesis. Perovskite nanowire photodetectors reached a responsivity of 10 A W^(-1)and a specific normalized detectivity of the order of 10^(12 )Jones. Different from most reported reviews focusing on photovoltaic applications, we summarize the rapid progress in the study of low-dimensional perovskite materials, as well as their promising applications in optoelectronic devices. In particular, we review the wide tunability of fabrication methods and the state-of-the-art research outputs of low-dimensional perovskite optoelectronic devices. Finally, the anticipated challenges and potential for this exciting research are proposed. | Jian Zhang Xiaokun Yang Hui Deng Keke Qiao Umar Farooq Muhammad Ishaq Fei Yi Huan Liu Jiang Tang Haisheng Song | 2017 | Nano-Micro Letters2017，9，3： | 10 |
| 10 | Atorvastatin improves insulin sensitivity in mice with obesity induced by monosodium glutamate显示文摘 | Ning ZHANG Yi HUAN Hui HUANG Guang-ming SONG Su-juan SUN Zhu-fang SHEN | 2010 | Acta Pharmacologica Sinica2010，31，1： | 9 |
| 11 | Regulating Effects of Novel CpG Chitosan-nanoparticles on Immune Responses of Mice to Porcine Paratyphoid Vaccines显示文摘Objective To study the regulating effects of a novel CpG oligodeoxynuleotide and the synergistic effect of chitosan-nanoparticles (CNP) with CpG on immune responses of mice, which were used to develop a novel immunoadjuvant to boost immune response to conventional vaccines. Methods A novel CpG ODN containing 11 CpG motifs was synthesized and its bioactivities to stimulate the proliferation of lymphocytes of pig in vitro were detected. Then it was entrapped with CNP prepared in our laboratory by the method of ionic cross linkage, and immunized Kunming mice were co-inoculated with paratyphoid vaccine. The peripheral blood was collected weekly from the tail vein of inoculated mice to detect the contents of IgG, IgA, IgM, and specific antibody against salmonella as well as the levels of interleukin-2 (IL2), IL-4, and IL-6 by SABC-ELISA assay. The numbers of leucocytes, monocytes, granuloytes, and lymphocytes were calculated separately using the routine method. The experimental mice were orally challenged with virulent salmonella 35 days after inoculation. Results This CpG ODN could remarkably provoke the proliferation of lymphocytes of pig in vitro in contrast with the control (P<0.05). Compared with those of the control, immunoglobulins, including IgG, IgA, IgM, and specific antibodies to paratyphoid vaccine, increased significantly in sera from the CpG or CpG-CNP-vaccinated mice (P<0.05). IL-2, IL-4, and IL-6 increased remarkably in sera from immunized mice (P<0.05). The leucocytes, monocytes, granuloytes, and lymphocytes of the mice immunized with CpG or CpG-CNP were also increased in number (P<0.05). After the challenge, these immunity values were elevated in the mice vaccinated with CpG or CpG-CNP. The immunized mice all survived, while the control mice fell ill with evident lesions with diffuse hemorrhage in stomach, small intestine, and peritoneum. Conclusions CpG ODN entrapped with CNP is a promising effective immunoadjuvant for vaccination, which promotes humoral and cellular immune responses, enhances immunity and resistance against salmonella by co-administration with paratyphoid vaccine. | MAN-LIANG FU SAN-CHENG YING MEI WU HUI LI KAI-YUAN WU YI YANG HUAN ZHANG CHI CHENG ZE-ZHOU WANG XIU-YING WANG XUE-BING LV YI-ZHENG ZHANG RONG GAO | 2006 | Biomedical and Environmental Sciences2006，19，4： | 8 |
| 12 | Magnetic nanoparticles based cancer therapy: current status and applications显示文摘Nanotechnology holds a promising potential for developing biomedical nanoplatforms in cancer therapy. The magnetic nanoparticles, which integrate uniquely appealing features of magnetic manipulation, nanoscale heat generator, localized magnetic field and enzyme-mimics, prompt the development and application of magnetic nanoparticles-based cancer medicine. Considerable success has been achieved in improving the magnetic resonance imaging(MRI) sensitivity, and the therapeutic function of the magnetic nanoparticles should be given adequate attention. This work reviews the current status and applications of magnetic nanoparticles based cancer therapy. The advantages of magnetic nanoparticles that may contribute to improved therapeutics efficacy of clinic cancer treatment are highlighted here. | Huan Zhang Xiao Li Liu Yi Fan Zhang Fei Gao Ga Long Li Yuan He Ming Li Peng Hai Ming Fan | 2018 | Science China(Life Sciences)2018，61，4： | 8 |
| 13 | Polymorphisms of angiotensin-converting enzyme 2 gene associated with magnitude of left ventricular hypertrophy in male patients with hypertrophic cardiomyopathy显示文摘背景:甚至带一个相同基因变化,内部家庭并且 intra 家庭变化当面全球被注意了并且在有亢奋的营养的心肌症(HCM ) 的病人的左心室肥大和猝死的严厉。修饰词基因可以贡献差异。变换血管收缩素的酶 2 (ACE2 ) 基因被建立了在社区与左心室肥大的参数被联系基于的男题目。现在的学习的目的是与 HCM 的显型调查 ACE2 基因多型性的协会。方法:261 个连续 HCM 病人和 609 健康控制的一个总数被注册进这研究。rs2106809 和 rs6632677 的多型性是由聚合酶链反应限制碎片长度多型性(PCR-RFLP ) 的 genotyped 并且由定序证实了。逻辑回归当模特儿,多,变量分析被用来决定机会比率(或) 并且为 HCM 的 ACE2 的变化的 95% 信心间隔(CI ) 。结果:rs2106809 的 T 等位基因和 rs6632677 的 C 等位基因为 HCM 授与增加的风险(或 1.34, 95% CI 1.01 - 1.77, P = 0.04;或 1.11 ,95% CI 1.03 - 1.21 , P = 0.002 ,分别地),并且 2 单个核苷酸多型性( SNP )在强壮的连接不平衡( LD ), TC haplotype 独立地与被联系一更高或为 HCM (或= 1.59 ,95% CI 1.21 - 1.87 )在为常规风险因素调整了以后。并且风险等位基因与被联系更厚内部 HCM 的室的氏族的厚度((20.0 +/- 6.3 ) 公里对(17.9 +/- 5.5 ) 公里, P = 0.03 并且(21.3 +/- 5.9 ) 公里对(17.9 +/- 5.8 ) 公里, P = 0.04,分别地) 。没有协会与 HCM 与女病人一起在二多型性之间被发现。结论:ACE2 基因力量的次要的等位基因是为在有 HCM 的男病人的左心室肥大的大小的基因修饰词。 | WANG Shu-xia FU Chun-yan ZOU Yu-bao WANG Hu SHI Yi XU Xi-qi CHEN Jing-zhou SONG Xiao-dong HUAN Tu-jun HUI Ru-tai | 2008 | Chinese Medical Journal2008，，1： | 8 |
| 14 | Fractionated stereotactic radiotherapy with vagina carotica protection technique for local residual nasopharyngeal carcinoma after primary radiotherapy显示文摘 | LIU Feng XIAO Jian-ping XU Ying-jie ZHANG Ye XU Guo-zhen GAO Li YI Jun-lin LUO Jing-wei HUANG Xiao-dong HUAN Fu-kui FANG Hao WAN Bao LI Ye-xiong | 2012 | Chinese Medical Journal2012，，14： | 8 |
| 15 | Fedora-type magnetic compression anastomosis device for intestinal anastomosis显示文摘BACKGROUND Although previous studies have confirmed the feasibility of magnetic compression anastomosis(MCA),there is still a risk of long-term anastomotic stenosis.For traditional MCA devices,a large device is associated with great pressure,and eventually increased leakage.AIM To develop a novel MCA device to simultaneously meet the requirements of pressure and size.METHODS Traditional nummular MCA devices of all possible sizes were used to conduct ileac anastomosis in rats.The mean(±SD)circumference of the ileum was 13.34±0.12 mm.Based on short-and long-term follow-up results,we determined the appropriate pressure range and minimum size.Thereafter,we introduced a novel“fedora-type”MCA device,which entailed the use of a nummular magnet with a larger sheet metal.RESULTS With traditional MCA devices,the anastomoses experienced stenosis and even closure during the long-term follow-up when the anastomat was smaller thanΦ5 mm.However,the risk of leakage increased when it was larger thanΦ4 mm.On comparison of the different designs,it was found that the“fedora-type”MCA device should be composed of aΦ4-mm nummular magnet with aΦ6-mm sheet metal.CONCLUSION The diameter of the MCA device should be greater than 120%of the enteric diameter.The novel“fedora-type”MCA device controls the pressure and optimizes the size. | Huan Chen Tao Ma Yue Wang Hao-Yang Zhu Zhe Feng Rong-Qian Wu Yi Lv Ding-Hui Dong | 2020 | World Journal of Gastroenterology2020，26，42： | 7 |
| 16 | Effects of Yue-Bi-Tang on water metabolism in severe acute pancreatitis rats with acute lung-kidney injury显示文摘BACKGROUND The complications acute lung injury and acute kidney injury caused by severe inflammation are the main reasons of high mortality of severe acute pancreatitis(SAP).These two complications can both lead to water metabolism and acid-base balance disorders,which could act as additional critical factors affecting the disease trend.Aquaporins(AQPs),which can regulate the transmembrane water transport,have been proved to participate in the pathophysiological process of SAP and the associated complications,such as acute lung injury and acute kidney injury.Thus,exploring herbs that can effectively regulate the expression of AQP in SAP could benefit the prognosis of this disease.AIM To determine whether Yue-Bi-Tang(YBT)can regulate the water metabolism in rats with severe acute pancreatitis via regulating the expression of aquaporins.METHODS Sprague-Dawley rats were randomly divided into three groups,sham operation group(SOG),model group(MG),and treatment group(TG).SAP was induced with 3.5%sodium taurocholate in the MG and TG.Rats in the TG were administered with YBT while SOG and MG rats were given the same volume of saline.Blood and tissue samples were harvested to detect serum inflammatory cytokines,histopathological changes,malondialdehyde and superoxide dismutase in the lung,and protein and mRNA expression of kidney injury molecule-1,α-smooth muscle actin,and vimentin in the kidney,and AQP1 and 4 in the lung,pancreas,and kidney.RESULTS The serum interleukin-10,tumor necrosis factorα,and creatinine levels were higher in the MG than in the SOG.Tumor necrosis factorαlevel in the TG was lower than that in the MG.Malondialdehyde level in lung tissues was higher than in the SOG.The pathological scores and edema scores of the pancreas,lung,and kidney tissues in the MG were all higher than those in the SOG and TG.The protein expression of AQP4 in lung tissues and AQP1 in kidney tissues in the MG were higher than those in the SOG and TG.The expression of vimentin was significantly higher in the MG than in the SOG.The expression of AQP1 mRNA in the lung and kidney,and AQP4 mRNA in the kidney was up-regulated in the MG compared to the SOG.CONCLUSION YBT might regulate water metabolism to reduce lung and kidney edema of SAP rats via decreasing AQP expression,and alleviate the tissue inflammatory injury. | Jing Hu Yu-Mei Zhang Yi-Fan Miao Lv Zhu Xiao-Lin Yi Huan Chen Xi-Jing Yang Mei-Hua Wan Wen-Fu Tang | 2020 | World Journal of Gastroenterology2020，26，43： | 7 |
| 17 | Blocking of N-acetylglucosaminyltransferase V induces cellular endoplasmic reticulum stress in human hepatocarcinoma 7721 cells显示文摘N-acetylglucosaminyltransferase V (GnT-V ) 是重要 tumorigenesis 和联系转移的酶。为了学习它的简历,工作, GnT-V 稳定地压制了房间线(GnT-V-AS/7,721 ) 在以前的学习从 7,721 个 hepatocarcinoma 房间被构造。在这研究, cDNA 数组基因表示侧面在 GnT-V-AS/7,721 之间被比较并且父母 7,721 个房间。数据显示 GnT-V-AS/7,721 显示出与 ER 压力一致的一个典型表达式模式。ER 压力的分子的机制被分析在二展开的蛋白质回答(UPR ) 在关键分子上在 GnT-V-AS/7,721 探索小径。为 ATF6 和 Ire1/XBP-1 小径,它被 BIP 的起来规定在 mRNA 和蛋白质证实 XBP-1 的拼接的形式的水平,和外观。至于 PERK/eIF2alpha 小径,激活嗯 eIF2alpha kinase 津贴被观察。从 GnT-V-AS/7,721 房间证实结果,在 UPR 的关键分子在 7,721 个房间再被检验由特定的 RNAi 处理防碍 GnT-V。结果与从 GnT-V-AS/7721 的那些是类似的,显示那块 GnT-V 能明确地激活嗯在 7,721 个房间的应力。率(3 )H 人加入与率改正了(3 ) 在 GnT-V-AS/7,721 的 H 列伊加入与控制相比极大地是下面调整的,它表明了综合的酶的缺乏的功能在堵住的 GnT-V 以后的 N-glycans。而且,与 underglycosylation 联系的女伴 GRP94 的更快的移居形式,和细胞内部的 glycoproteins 的广泛地改变的 N-glycans 结构是也在 GnT-V-AS/7,721 检测了。这些结果支持了机制损害的那块 GnT-V 表达式女伴和 N-glycan-synthesizing 酶工作,它在 vivo 引起了 UPR。 | Huan Fang Wei Huang Ying Ying Xu Zong Hou Shen Chao Qun Wu Shou Yi Qiao Yan Xu Long Yu Hui Li Chen | 2006 | Cell Research2006，16，1： | 7 |
| 18 | Multiple organ dysfunction syndrome due to massive wasp stings: an autopsy case report显示文摘 | ZHANG Ling TANG Yi LIU Fang SHI Yu-ying CAO Yu XU Huan FU Ping | 2012 | Chinese Medical Journal2012，，11： | 7 |
| 19 | Rapid identification of chemical profile in Gandou decoction by UPLCQ-TOF-MSE coupled with novel informatics UNIFI platform显示文摘Gandou decoction(GDD),a well-known traditional Chinese medicine(TCM)formula,has been widely used for decades to treat Wilson's disease(WD)in China due to its remarkable clinical effects.However,the chemical constituents of GDD still remain unclear because of their complexity.In this work,a reliable and sensitive strategy based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MSE)and UNIFI informatics platform was applied to investigate the chemical components in GDD.In total,96 compounds including anthraquinones,alkaloids,protostane triterpenoids,flavonoids,triterpenoid saponins,tannins,curcuminoids,etc,were identified or tentatively characterized from GDD by comparing their retention time,accurate mass within 5 ppm error and MSE fragmentation patterns.Among them,eleven compounds were confirmed unambiguously with reference standards.Representative compounds in different chemical structure types were analyzed in fragmentation patterns and characteristic ions.Moreover,to better understand the chemical contribution of individual herbs to the whole decoction,the corresponding each herb in GDD was also detected.This study developed a rapid method for characterizing the chemical constituents in GDD,which could not only be used for chemical standardization and quality control,but also be helpful for further research of GDD in vivo. | Li Xu Yi Liu Hongfei Wu Huan Wu Xiaochuang Liu An Zhou | 2020 | Journal of Pharmaceutical Analysis2020，10，1： | 6 |
| 20 | Could neoadjuvant chemotherapy increase postoperative complication risk of laparoscopic total gastrectomy? A monoinstitutional propensity score-matched study in China显示文摘The potential survival benefit of neoadjuvant chemotherapy(NC)in patients with advanced gastric cancer has been widely recognized.With the development of minimally invasive surgery,which is represented by laparoscopy,the effect of NC on the safety of laparoscopic gastrectomy remains to be further explored.AIM To compare the short-term outcomes of laparoscopic total gastrectomy(LTG)after NC(NC-LTG)with LTG alone.METHODS A total of 92 patients who underwent NC-LTG and 381 patients who received LTG alone at the Chinese PLA General Hospital between September 2015 and September 2020 were retrospectively included in our study.We used propensityscore matching(PSM)to balance baseline bias.After 1:1 PSM,73 patients were included in each group with no statistically significant difference in baseline characteristics.RESULTS The NC-LTG group exhibited a longer operation time(244.10±48.13 min vs 225.74±45.33 min,P=0.019)and increased intraoperative blood loss[150(100-300)mL vs 100(100-200)mL,P=0.011]compared to the LTG group.The 30-d postoperative morbidity of the NC-LTG group was 20.5%(15/73),and that of the LTG group was 13.7%(10/73).There were no significant differences in 30-d severe complication rates or anastomotic leakage rates.Subgroup analysis showed that the patients with pTNM(pathological tumor-node-metastasis classification)T0N0-II in the NC-LTG group underwent a longer operation than the LTG group,while no significant difference was found in any perioperative index for the pTNM III patients.A multivariate analysis showed that an operation time longer than 240 min was an independent risk factor(odds ratio=3.021,95%confidence interval:1.160-7.868,P=0.024),while NC was not an independent risk factor for postoperative complications in LTG.CONCLUSION Despite a longer operation time and more blood loss after NC-LTG,which indicate surgical difficulty,NC-LTG exhibits acceptable short-term outcomes compared to LTG,suggesting the safety and feasibility of NC-LTG. | Hao Cui Jian-Xin Cui Yu-Ning Wang Bo Cao Huan Deng Ke-Cheng Zhang Tian-Yu Xie Wen-Quan Liang Yi Liu Lin Chen Bo Wei | 2021 | World Journal of Gastrointestinal Surgery2021，13，5： | 5 |
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