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| 1 | High-quality genome assembly of 'Cuiguan' pear (Pyrus pyrifolia) as a reference genome for identifying regulatory genes and epigenetic modifications responsible for bud dormancy显示文摘Dormancy-associated MADS-box(DAM)genes serve as crucial regulators of the endodormancy cycle in rosaceous plants.Although pear DAM genes have been identified previously,the lack of a high-quality reference genome and techniques to study gene function have prevented accurate genome-wide analysis and functional verification of such genes.Additionally,the contribution of other genes to the regulation of endodormancy release remains poorly understood.In this study,a high-quality genome assembly for'Cuiguan'pear(Pyrus pyrifolia),which is a leading cultivar with a low chilling requirement cultivated in China,was constructed using PacBio and Hi-C technologies.Using this genome sequence,we revealed that pear DAM genes were tandemly clustered on Chr8 and Chr15 and were differentially expressed in the buds between'Cuiguan'and the high-chilling-requirement cultivar'Suli'during the dormancy cycle.Using a virus-induced gene silencing system,we determined the repressive effects of DAM genes on bud break.Several novel genes potentially involved in the regulation of endodormancy release were identified by RNA sequencing and H3K4me3 chromatin immunoprecipitation sequencing analyses of‘Suli'buds during artificial chilling using the new reference genome.Our findings enrich the knowledge of the regulatory mechanism underlying endodormancy release and chilling requirements and provide a foundation for the practical regulation of dormancy release in fruit trees as an adaptation to climate change. | Yuhao Gao Qinsong Yang Xinhui Yan Xinyue Wu Feng Yang Jianzhao Li Jia Wei Junbei Ni Mudassar Ahmad Songling Bai Yuanwen Teng | 2021 | Horticulture Research2021，8，1： | 5 |
| 2 | Carbon inputs regulate the temperature sensitivity of soil respiration in temperate forests显示文摘Litter and root activities may alter the temperature sensitivity(Q_(10))of soil respiration.However,existing studies have not provided a comprehensive understanding of the effects of litter and root carbon inputs on the Q_(10)of soil respiration in different seasons.In this study,we used the trench method under in situ conditions to measure the total soil respiration(R_(total)),litter-removed soil respiration(R_(no-litter)),root-removed soil respiration(R_(no-root)),and the decomposition of soil organic matter(i.e.,both litter and root removal;R_(SOM))in different seasons of pioneer(Populus davidiana Dode)and climax(Quercus liaotungensis Mary)forests on the Loess Plateau,China.Soil temperature,soil moisture,litter biomass,fine root biomass,litter carbon,and root carbon were analyzed to obtain the drive mechanism of the Q_(10)of soil respiration in the two forests.The results showed that the Q_(10)of soil respiration exhibited seasonality,and the Q_(10)of soil respiration was higher in summer.The litter enhanced the Q_(10)of soil respiration considerably more than the root did.Soil temperature,soil moisture,fine root biomass,and litter carbon were the main factors used to predict the Q_(10)of different soil respiration components.These findings indicated that factors affecting the Q_(10)of soil respiration highly depended on soil temperature and soil moisture as well as related litter and root traits in the two forests,which can improve our understanding of soil carbon–climate feedback in global warming.The results of this study can provide reference for exploring soil respiration under temperate forest restoration. | LIU Yulin LI Jiwei HAI Xuying WU Jianzhao DONG Lingbo PAN Yingjie SHANGGUAN Zhouping WANG Kaibo DENG Lei | 2022 | Journal of Arid Land2022，14，9： | 3 |
| 3 | Cryo-EM structures of human m^(6)A writer complexes显示文摘N6-methyladenosine(m^(6)A)is the most abundant ribonucleotide modification among eukaryotic messenger RNAs.The m^(6)A“writer”consists of the catalytic subunit m^(6)A-METTL complex(MAC)and the regulatory subunit m^(6)A-METTL-associated complex(MACOM),the latter being essential for enzymatic activity.Here,we report the cryo-electron microscopy(cryo-EM)structures of MACOM at a 3.0-Åresolution,uncovering that WTAP and VIRMA form the core structure of MACOM and that ZC3H13 stretches the conformation by binding VIRMA.Furthermore,the 4.4-Åresolution cryo-EM map of the MACOM–MAC complex,combined with crosslinking mass spectrometry and GST pull-down analysis,elucidates a plausible model of the m^(6)A writer complex,in which MACOM binds to MAC mainly through WTAP and METTL3 interactions.In combination with in vitro RNA substrate binding and m^(6)A methyltransferase activity assays,our results illustrate the molecular basis of how MACOM assembles and interacts with MAC to form an active m^(6)A writer complex. | Shichen Su Shanshan Li Ting Deng Minsong Gao Yue Yin Baixing Wu Chao Peng Jianzhao Liu Jinbiao Ma Kaiming Zhang | 2022 | Cell Research2022，32，11： | 3 |
| 4 | Dietary fibers with low hydration properties exacerbate diarrhea and impair intestinal health and nutrient digestibility in weaned piglets显示文摘Background:This study aimed to investigate the hydration properties of different-source fibrous materials by com-paring their water-binding capacity(WBC),water swelling capacity(WSC),viscosity,and in vivo effects of selected samples on growth performance,nutrient digestibility,diarrhea,and intestinal health in weaned piglets.Methods:A total of 13 commercially available fibrous materials were first compared in chemical composition and in vitro hydration property.Subsequently,40 weaned piglets were randomized to five experimental dietary groups(8 piglets per group):control diet(a basal diet without dietary fiber,CON),basal diet supplemented with 5%microcrys-talline cellulose(MCC),5%wheat bran(WB),5%Moringa oleifera leaf powder(MOLP),or 5%sugar beet pulp(SBP),fol-lowed by analyzing their growth performance and diarrhea rate in a 28-d experiment.After the feeding experiment,anaesthetized piglets were killed,and their intestinal and colon content or plasma samples were analyzed in nutrient digestibility,intestinal morphology,intestinal barrier,short-chain fatty acids(SCFAs),and bacterial population.Results:In vitro studies showed low hydration properties for WB and MCC,while medium hydration properties for MOLP and SBP.In vivo studies indicated that compared with medium hydration property groups,low hydration prop-erty groups showed(1)exacerbated diarrhea,impaired intestinal health,and reduced apparent fecal digestibility of dry matter,gross energy,acid detergent fiber,and neutral detergent fiber;(2)decreased SCFAs concentration and rela-tive levels of Lactobacillus and Bifidobacterium,but increased levels of Escherichia coli and Brachyspira hyodysenteriae in colon contents.Additionally,SBP showed optimal performance in reducing diarrhea and increasing SCFAs produc-tion.Correlation analysis revealed a positive correlation of fiber hydration properties with in vitro SCFAs production,and diarrhea index and nutrient digestibility were negatively and positively correlated with SCFAs levels in the colon contents of weaned piglets,respectively.Conclusions:Different-source dietary fibers varied in their hydration properties and impacts on diarrhea,microbial composition and SCFAs production in weaned piglets.WB and MCC could exacerbate diarrhea and impair nutrient digestibility,probably because their low hydration properties were detrimental to gut microbial homeostasis and fermentation.Our findings provide new ideas for rational use of fiber resources in weaned piglets. | Shuangbo Huang Zhijuan Cui Xiangyu Hao Chuanhui Cheng Jianzhao Chen Deyuan Wu Hefeng Luo Jinping Deng Chengquan Tan | 2023 | Journal of Animal Science and Biotechnology2023，14，2： | 1 |
| 5 | Horizontal convection in a rectangular enclosure driven by a linear temperature profile显示文摘The horizontal convection in a square enclosure driven by a linear temperature profile along the bottom boundary is investigated numerically by using a finite difference method.The Prandtl number is fixed at 4.38,and the Rayleigh number Ra ranges from107 to 1011.The convective flow is steady at a relatively low Rayleigh number,and no thermal plume is observed,whereas it transits to be unsteady when the Rayleigh number increases beyond the critical value.The scaling law for the Nusselt number Nu changes from Rossby’s scaling Nu~Ra^(1/5)in a steady regime to Nu~Ra^(1/4)in an unsteady regime,which agrees well with the theoretically predicted results.Accordingly,the Reynolds number Re scaling varies from Re~Ra^(3/11)to Re~Ra^(2/5).The investigation on the mean flows shows that the thermal and kinetic boundary layer thickness and the mean temperature in the bulk zone decrease with the increasing Ra.The intensity of fluctuating velocity increases with the increasing Ra. | Tianyong YANG Bofu WANG Jianzhao WU Zhiming LU Quan ZHOU | 2021 | Applied Mathematics and Mechanics(English Edition)2021，42，8： | 1 |
| 6 | MyD88 exacerbates immunological pathology in experimental viral fulminant hepatitis显示文摘Objective To explore the role of MyD88 signaling in MHV-3 virus-mediated fulminant hepatitis. Methods We evaluated liver lesion status, the expression of multiple pro-inflammatory cytokines and HMGB1, the recruitment of inflammatory ILC3, and mortality in MyD88-/-and WT mice. Results The expression of multiple pro-inflammatory cytokines that recruit inflammatory ILC3 to the liver was severely impaired in MyD88-/-mice resulting in reduced liver pathology, viral replication, and mortality post-infection. Additionally, MHV-3 markedly increased the expression of high-mobility group box 1(HMGB1) in infected hepatocytes/macrophages and induced HMGB1 protein migration from the nucleus to the extracellular milieu, where it activates MyD88-dependent inflammation. Conclusion Our findings indicate that MyD88 exacerbates immunological pathology in experimental viral fulminant hepatitis. | Jianzhao Deng Qin Ning Weiming Yan Xuan Yang Lizhen Zhao Yuzhang Wu Bei Zhang | 2019 | Oncology and Translational Medicine2019，5，2： | 1 |
| 7 | 一种独特的“保护-被保护”策略在嘌呤类核苷类药物生物合成中被破解显示文摘文章简介喷司他丁(Pentostain)是美国FDA批准治疗白血病的一线药物,而阿糖腺苷(Vidarabine)则在临床上广泛用来治疗疱疹病毒、巨细胞病毒及乙型肝炎病毒所引起的疾病。 | Pan Wu Dan Wan Gudan Xu Gui Wang Hongmin Ma Tingting Wang Yaojie Gao Jianzhao Qi Xiaoxia Chen Jian Zhu 李永泉 Zixin Deng 陈文青 | 2018 | 科学新闻2018，0，4： | 0 |
| 8 | Electromagnetic Environmental Impact of 500-kV Double -Circuit Transmission Lines显示文摘The strength of the power frequency electric intensity and magnetic field of the 500-kV double circuit transmission lines was calculated by using the equivalent charge method and the Ampere's Law, and the environmental impact factors of the fields were evaluated. By optimizing the phase sequence, the frequency electric intensity and magnetic field strength can be reduced. Within a distance of 25 m from the center of the transmission line, the power frequency electric intensity and magnetic field strength fall off sharply with the distance increase. Finally, the best phase sequence and the minimum ground clearance of the transmission lines were obtained to meet the requirements of the least impact on envionment. | Qi Jianzhao, Zhang Guohua, Wu Xiaoyan Hebei Electric Power Design & Research Institute Zhang Jianping | 2011 | Electricity2011，22，1： | 0 |
| 9 | Dietary adenosine supplementation improves placental angiogenesis in IUGR piglets by up-regulating adenosine A2a receptor显示文摘Abnormal placental angiogenesis is associated with the occurrence of intrauterine growth restriction(IUGR)in piglets,and effective treatment strategies against this occurrence remain to be explored.Adenosine has been reported to play an important role in angiogenesis,but its role in placental angio-genesis is still unknown.Here,we investigated the effect of dietary adenosine supplementation on IUGR occurrence in piglets by analyzing the role of adenosine in placental angiogenesis for Normal and IUGR piglets.Specifically,88 sows were allotted to 2 treatments(n=44)and fed a basal diet supplemented with 0%or 0.1%of adenosine from day 65 of gestation until farrowing,followed by collecting the placental samples of Normal and IUGR piglets,and recording their characteristics.The results showed that adenosine supplementation increased the mean birth weight of piglets(P<0.05)and placental efficiency(P<0.05),while decreasing the IUGR piglet rate(P<0.05).Expectedly,the placenta for IUGR neonates showed a down-regulated vascular density(P<0.05)and angiogenesis as evidenced by the expression level of vascular cell adhesion molecule-1(VCAM1)(P<0.05).Notably,dietary adenosine supplementation promoted angiogenesis(P<0.05)both in the Normal and IUGR placenta.More importantly,the expression level of adenosine A2a receptor(ADORA2A)was lower(P<0.05)in the IUGR placenta than in Normal placenta,whereas adenosine treatment could significantly increase ADORA2A expression,and also had an interaction effect between factors IUGR and Ado.Collectively,placentae for IUGR piglets showed impaired angiogenesis and down-regulated expression level of ADORA2A,while dietary adenosine supplementation could activate ADORA2A expression,improve the placental angio-genesis,and ultimately decrease the occurrence of IUGR in piglets. | Zifang Wu Jiawei Nie Deyuan Wu Shuangbo Huang Jianzhao Chen Huajin Liang Xiangyu Hao Li Feng Hefeng Luo Chengquan Tan | 2023 | Animal Nutrition2023，，2： | 0 |
| 10 | Key progresses of MOE key laboratory of macromolecular synthesis and functionalization in 2020显示文摘In 2020, the MOE Key Laboratory of Macromolecular Synthesis and Functionalization in Zhejiang University had made progresses in several aspects. First, a series of metal-free organoboron catalysts had been designed and synthesized facilely, exhibiting outstanding reactivity, thermalstability and productivity in different kinds of polymerization and cycloaddition reactions. Second, a variety of chalcogen(O, S, Se)-rich polymers had been synthesized via organocatalysis and fabricated to be the ionic conductive and photoluminescent materials. Third, diverse microenvironment-sensitive nanoparticles had been designed,and novel strategies had been realized, to enhance the therapeutic efficacy in cancer as well as biofilmassociated infections. Fourth, m6 A modification on cellular transcriptome-wide messenger RNA had been successfully mapped at single base resolution using a metabolic labeling method. Fifth, a hydrogel-based robot had been developed, showing swift locomotion as a response to dynamic light stimulations. Sixth,the conformation-size scaling law and the conformation evolution map of 2 D macromolecules in solution had been elucidated experimentally, in the single-layer graphene oxide model. Seventh, semitransparent polymer solar cells, promising as building-integrated photovoltaics, have been developed with the fine balance among power conversion efficiency, visible light transparency and infrared photon radiation rejection. Finally, long-range ordered bulk-heterojunctions of organic semiconductors had been achieved,and their superior optoelectronic properties and potential application in photoelectric conversion had been revealed. The related work progresses are reviewed in this paper. | Jie Ren Xiao Shu Ya Wang Di Wang Guangpeng Wu Xinghong Zhang Qiao Jin Jianzhao Liu Ziliang Wu Zhen Xu Chang-Zhi Li Hanying Li | 2022 | Chinese Chemical Letters2022，33，4： | 0 |
| 11 | Monte Carlo Simulation-Based Robust Workflow Scheduling for Spot Instances in Cloud Environments显示文摘When deploying workflows in cloud environments,the use of Spot Instances(SIs)is intriguing as they are much cheaper than on-demand ones.However,Sls are volatile and may be revoked at any time,which results in a more challenging scheduling problem involving execution interruption and hence hinders the successful handling of conventional cloud workflow scheduling techniques.Although some scheduling methods for Sls have been proposed,most of them are no more applicable to the latest Sls,as they have evolved by eliminating bidding and simplifying the pricing model.This study focuses on how to minimize the execution cost with a deadline constraint when deploying a workflow on volatile Sls in cloud environments.Based on Monte Carlo simulation and list scheduling,a stochastic scheduling method called MCLS is devised to optimize a utility function introduced for this problem.With the Monte Carlo simulation framework,MCLS employs sampled task execution time to build solutions via deadline distribution and list scheduling,and then returns the most robust solution from all the candidates with a specific evaluation mechanism and selection criteria.Experimental results show that the performance of MCLS is more competitive comparedwithtraditionalalgorithms. | Quanwang Wu Jianzhao Fang Jie Zeng Junhao Wen Fengji Luo | 2024 | Tsinghua Science and Technology2024，29，1： | 0 |
| 12 | Manipulated precipitation regulated carbon and phosphorus limitations of microbial metabolisms in a temperate grassland on the Loess Plateau,China显示文摘Manipulated precipitation patterns can profoundly influence the metabolism of soil microorganisms.However,the responses of soil organic carbon(SOC)and nutrient turnover to microbial metabolic limitation under changing precipitation conditions remain unclear in semi-arid ecosystems.This study measured the potential activities of enzymes associated with carbon(C:β-1,4-glucosidase(BG)andβ-D-cellobiosidase(CBH)),nitrogen(N:β-1,4-N-acetylglucosaminidase(NAG)and L-leucine aminopeptidase(LAP))and phosphorus(P:alkaline phosphatase(AP))acquisition,to quantify soil microbial metabolic limitations using enzymatic stoichiometry,and then identify the implications for soil microbial metabolic limitations and carbon use efficiency(CUE)under decreased precipitation by 50%(DP)and increased precipitation by 50%(IP)in a temperate grassland.The results showed that soil C and P were the major elements limiting soil microbial metabolism in temperate grasslands.There was a strong positive dependence between microbial C and P limitations under manipulated precipitation.Microbial metabolism limitation was promoted by DP treatment but reversed by IP treatment.Moreover,CUE was inhibited by DP treatment but promoted by IP treatment.Soil microbial metabolism limitation was mainly regulated by soil moisture and soil C,N,and P stoichiometry,followed by available nutrients(i.e.,NO^(-)_(3),NH^(+)_(4),and dissolved organic C)and microbial biomass(i.e.,MBC and MBN).Overall,these findings highlight the potential role of changing precipitation in regulating ecosystem C turnover by limiting microbial metabolism and CUE in temperate grassland ecosystems. | HAI Xuying LI Jiwei LIU Yulin WU Jianzhao LI Jianping SHANGGUAN Zhouping DENG Lei | 2022 | Journal of Arid Land2022，14，10： | 0 |
| 13 | Key progresses of MOE key laboratory of macromolecular synthesis and functionalization in 2022显示文摘In 2022,The MOE Key Laboratory of Macromolecular Synthesis and Functionalization in Zhejiang University had achieved several important results.First,a series of well-defined dinuclear organoboron catalysts were developed to precisely control the enchainment of ether and carbonate segments during the copolymerization of CO_(2)and epoxides.Second,polyester had been synthesized through cationic copolymerization of cyclic anhydride.Third,ring-opening polymerization of carbon dioxide based valerolactone had been achieved,revealing the prospect of 3-ethylidene-6-vinyltetrahydro-2H-pyran-2-one(EVL)in utilizing CO_(2)and synthesizing functional polymers.Fourth,machine learning methods have been applied to biomaterial research,enabling high-throughput screening of functional biomaterial surfaces for implantable devices,and searching for potent antimicrobial peptides in whole combinatorial peptide libraries.Fifth,methods of characterization of biomacromolecule RNA transcription and manipulation of nucleoside modification were developed.Sixth,artificial enzymes-armed Bifidobacterium Longum probiotics were established to tune down gut inflammation.Seventh,three-dimensional(3D)printing technologies were used to engineer tough supramolecular hydrogels.Eighth,hydroplastic foaming graphene frameworks for acoustic and conductive polymer composites were provided for application.Ninth,aggregate photophysics about the nature of through-space interactions(TSIs)and manipulating their strength in small molecules with non-conjugated structure had been elucidated.Tenth,the forming mechanism of a newfound nested texture in poly(L-lactic acid)(PLLA)spherulitic films had been revealed.Finally,the isotropically dyeing mechanism of KDP single crystals grown from hydrogels have been explored.The related works are reviewed in this paper. | Xumeng Deng Kaihao Chen Kai Pang Xiaoting Liu Minsong Gao Jie Ren Guanwen Yang Guangpeng Wu Chengjian Zhang Xufeng Ni Peng Zhang Jian Ji Jianzhao Liu Zhengwei Mao Ziliang Wu Zhen Xu Haoke Zhang Hanying Li | 2024 | Chinese Chemical Letters2024，35，3： | 0 |
| 14 | Soil microbial respiration is regulated by stoichiometric imbalances: Evidence from a humidity gradient case显示文摘Humidity not only affects soil microbial respiration(SMR) directly, but, indirectly by regulating the availability of soil water and nutrients. However,the patterns of direct and indirect effects of humidity on SMR over large precipitation gradients remain unclear, limiting our understanding of the effects of precipitation changes on soil C cycle. Here, we investigated the relationships among humidity, soil nutrients, and SMR by identifying stoichiometric imbalances, microbial elemental homeostasis, and microbial C use efficiency along a precipitation gradient at a continental scale. The relationship between SMR and humidity index(HI) corresponded to a Richard’s curve with an inflection point threshold value of approximately 0.7. Soil microbial respiration increased with increasing humidity in drier areas(HI < 0.7), but tended to balance above this threshold. Increasing humidity exacerbated C:P and N:P imbalances across the selected gradient. Severe N and P limitations in soil microbial communities were observed in drier areas, while soil microbes suffered from aggravated P limitation as the humidity increased in wetter areas(HI > 0.7). Soil microbial communities regulated their enzyme production to maintain a strong stoichiometric homeostasis in drier areas;enzyme production, microbial biomass, and threshold elemental ratios were non-homeostatic under P limitation in wetter areas, which further contributed to the increase in SMR. Our results identified a moisture constraint on SMR in drier areas and highlighted the importance of nutrient(especially for P) limitations induced by humidity in regulating SMR in wetter areas. Understanding the modulation of SMR via soil enzyme activity may improve the prediction of soil C budget under future global climate change. | Jiwei LI Jiangbo XIE Jianzhao WU Yongxing CUI Lingbo DONG Yulin LIU Xuying HAI Yan LI Zhouping SHANGGUAN Kaibo WANG Changhui PENG Lei DENG | 2023 | Pedosphere2023，33，6： | 0 |
| 15 | Processing parameter optimization of fiber laser beam welding using an ensemble of metamodels and MOABC显示文摘In fiber laser beam welding(LBW),the selection of optimal processing parameters is challenging and plays a key role in improving the bead geometry and welding quality.This study proposes a multi-objective optimization framework by combining an ensemble of metamodels(EMs)with the multi-objective artificial bee colony algorithm(MOABC)to identify the optimal welding parameters.An inverse proportional weighting method that considers the leave-one-out prediction error is presented to construct EM,which incorporates the competitive strengths of three metamodels.EM constructs the correlation between processing parameters(laser power,welding speed,and distance defocus)and bead geometries(bead width,depth of penetration,neck width,and neck depth)with average errors of 10.95%,7.04%,7.63%,and 8.62%,respectively.On the basis of EM,MOABC is employed to approximate the Pareto front,and verification experiments show that the relative errors are less than 14.67%.Furthermore,the main effect and the interaction effect of processing parameters on bead geometries are studied.Results demonstrate that the proposed EM-MOABC is effective in guiding actual fiber LBW applications. | Jianzhao WU Chaoyong ZHANG Kunlei LIAN Jiahao SUN Shuaikun ZHANG | 2022 | Frontiers of Mechanical Engineering2022，17，4： | 0 |
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