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| 1 | CAS9 is a genome mutator by directly disrupting DNA-PK dependent DNA repair pathway显示文摘With its high efficiency for site-specific genome editing and easy manipulation,the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR associated protein 9(CAS9)system has become the most widely used gene editing technology in biomedical research.In addition,significant progress has been made for the clinical development of CRISPR/CAS9 based gene therapies of human diseases,several of which are entering clinical trials.Here we report that CAS9 protein can function as a genome mutator independent of any exogenous guide RNA(gRNA)in human cells,promoting genomic DNA double-stranded break(DSB)damage and genomic instability.CAS9 interacts with the KU86 subunit of the DNA-dependent protein kinase(DNA-PK)complex and disrupts the interaction between KU86 and its kinase subunit,leading to defective DNA-PK-dependent repair of DNA DSB damage via non-homologous end-joining(NHEJ)pathway.XCAS9 is a CAS9 variant with potentially higher fidelity and broader compatibility,and dCAS9 is a CAS9 variant without nuclease activity.We show that XCAS9 and dCAS9 also interact with KU86 and disrupt DNA DSB repair.Considering the critical roles of DNA-PK in maintaining genomic stability and the pleiotropic impact of DNA DSB damage responses on cellular proliferation and survival,our findings caution the interpretation of data involving CRISPR/CAS9-based gene editing and raise serious safety concerns of CRISPR/CAS9 system in clinical application. | Shuxiang Xu Jinchul Kim Qingshuang Tang Qu Chen Jingfeng Liu Yang Xu Xuemei Fu | 2020 | Protein & Cell2020，11，5： | 8 |
| 2 | The temporal and spatial fluctuation of the groundwater level along the Tarim Desert Highway显示文摘In this work, a groundwater monitor section along the Tarim Desert Highway was set up, and the groundwater level data were recorded. The temporal and spatial fluctuations of the groundwater level were analyzed based on the groundwater level measurements, hydrologic data, the groundwater/surface water interconversion theory and the groundwater dynamics. The spatial distribution of groundwater is mostly affected by sand dune height and landform variation. The four primary temporal influence factors of groundwater level can be ordered in decreasing significance as: shelterbelt pumping > vertical leakage > river flow change > runoff replenishment. The runoff replenishment has the biggest influence range, and the shelterbelt pumping has the smallest influence range. The groundwater level fluctuates annually in a natural pattern. | FAN JingLong XU XinWen LEI JaQiang ZHAO JingFeng LI ShengYu WANG HaiFeng ZHANG JianGuo ZHOU HongWei | 2008 | Chinese Science Bulletin2008，53，S2： | 6 |
| 3 | OPTIMAL COMBINATIONAL OF QUOTA-SHARE AND STOP-LOSS REINSURANCE CONTRACTS UNDER VAR AND CTE WITH A CONSTRAINED REINSURANCE PREMIUM显示文摘这份报纸考虑最小化 VaR 和 CTE 的问题一保险公司由控制 combinational 限额份额和站损失再保险策略保留了风险。与抑制再保险奖赏,作者分别地在站损失再保险和站损失 after-quota-share 再保险的盒子以后在限额份额的情况中给保留的风险的明确的再保险形式和最小的 VaR 和 CTE。最后,作者断定在站损失以后的限额份额比在限额份额以后的站损失是更好的再保险策略与一样的抑制再保险奖赏最小化 VaR 和 CTE。并且纯站损失再保险与一个高级规章的要求为一个保险公司被比较喜欢。 | Ming ZHOU Hongbin DONG Jingfeng XU | 2011 | Journal of Systems Science & Complexity2011，24，1： | 4 |
| 4 | Chinese expert consensus on conversion therapy for hepatocellular carcinoma(2021 edition)显示文摘Recent advances in systemic and locoregional treatments for patients with unresectable or advanced hepatocellular carcinoma(HCC)have resulted in improved response rates.This has provided an opportunity for selected patients with initially unresectable HCC to achieve adequate tumor downstaging to undergo surgical resection,a‘conversion therapy’strategy.However,conversion therapy is a new approach to the treatment of HCC and its practice and treatment protocols are still being developed.Review the evidence for conversion therapy in HCC and develop consensus statements to guide clinical practice.Evidence review:Many research centers in China have accumulated significant experience implementing HCC conversion therapy.Preliminary findings and data have shown that conversion therapy represents an important strategy to maximize the survival of selected patients with intermediate stage to advanced HCC;however,there are still many urgent clinical and scientific challenges for this therapeutic strategy and its related fields.In order to summarize and learn from past experience and review current challenges,the Chinese Expert Consensus on Conversion Therapy for Hepatocellular Carcinoma(2021 Edition)was developed based on a review of preliminary experience and clinical data from Chinese and non-Chinese studies in this field and combined with recommendations for clinical practice.Sixteen consensus statements on the implementation of conversion therapy for HCC were developed.The statements generated in this review are based on a review of clinical evidence and real clinical experience and will help guide future progress in conversion therapy for patients with HCC. | Hui-Chuan Sun Jian Zhou Zheng Wang Xiufeng Liu Qing Xie Weidong Jia Ming Zhao Xinyu Bi Gong Li Xueli Bai Yuan Ji Li Xu Xiao-Dong Zhu Dousheng Bai Yajin Chen Yongjun Chen Chaoliu Dai Rongping Guo Wenzhi Guo Chunyi Hao Tao Huang Zhiyong Huang Deyu Li Gang Li Tao Li Xiangcheng Li Guangming Li Xiao Liang Jingfeng Liu Fubao Liu Shichun Lu Zheng Lu Weifu Lv Yilei Mao Guoliang Shao Yinghong Shi Tianqiang Song Guang Tan Yunqiang Tang Kaishan Tao Chidan Wan Guangyi Wang Lu Wang Shunxiang Wang Tianfu Wen Baocai Xing Bangde Xiang Sheng Yan Dinghua Yang Guowen Yin Tao Yin Zhenyu Yin Zhengping Yu Bixiang Zhang Jialin Zhang Shuijun Zhang Ti Zhang Yamin Zhang Yubao Zhang Aibin Zhang Haitao Zhao Ledu Zhou Wu Zhang Zhenyu Zhu Shukui Qin Feng Shen Xiujun Cai Gaojun Teng Jianqiang Cai Minshan Chen Qiang Li Lianxin Liu Weilin Wang Tingbo Liang Jiahong Dong Xiaoping Chen Xuehao Wang Shusen Zheng Jia Fan 无 | 2022 | Hepatobiliary Surgery and Nutrition2022，11，2： | 4 |
| 5 | Functions of p53 in pluripotent stem cells显示文摘Pluripotent stem cells(PSCs)are capable of unlimited self-renewal in culture and differentiation into all functional cell types in the body,and thus hold great promise for regenerative medicine.To achieve their clinical potential,it is critical for PSCs to maintain genomic stability during the extended proliferation.The critical tumor suppressor p53 is required to maintain genomic stability of mammalian cells.In response to DNA damage or oncogenic stress,p53 plays multiple roles in maintaining genomic stability of somatic cells by inducing cell cycle arrest,apoptosis,and senescence to prevent the passage of genetic mutations to the daughter cells.p53 is also required to maintain the genomic stability of PSCs.However,in response to the genotoxic stresses,a primary role of p53 in PSCs is to induce the differentiation of PSCs and inhibit pluripotency,providing mechanisms to maintain the genomic stability of the self-renewing PSCs.In addition,the roles of p53 in cellular metabolism might also contribute to genomic stability of PSCs by limiting oxidative stress.In summary,the elucidation of the roles of p53 in PSCs will be a prerequisite for developing safe PSC-based cell therapy. | Xuemei Fu Shouhai Wu Bo Li Yang Xu Jingfeng Liu | 2020 | Protein & Cell2020，11，1： | 4 |
| 6 | Orthorectification of IKONOS and Impact of Different Resolution DEM显示文摘IKONOS image has been wildly used in city planning, precision agriculture and emergence response. However, the accuracy of IKONOS Geo product is limited due to distortion caused by terrain relief. Orthorectification was performed to remove the distortion and the impact of different DEM on orthorectification were evaluated. 38 ground control points (GCPs) and 25 independent check points (ICPs) were collected. DEMs were generated from 1∶10 000 and 1∶50 000 topographic maps. Results show that RMS error at the check points is 1.554 0 m using DEM generated from 1∶10 000 topographic map, which can meet the accuracy requirement of IKONOS Precision product (1.9 m RMSE). While RMS error is 2.572 4 m using DEM generated from 1∶50 000 topographic map. | XU Junfeng HUANG Jingfeng | 2006 | Geo-Spatial Information Science2006，9，2： | 2 |
| 7 | The template-assisted zinc ion incorporation in SAPO-34 and the enhanced ethylene selectivity in MTO reaction显示文摘The SAPO-34 catalyst was fine-tuned with zinc cations through a straightforward template-assisted ion incorporation (TH) process, without the necessary template pre-removal and the preparation of NH4- SAPO-34 intermediate, which is more facile, efficient and cost-effective than the conventional ion exchange process. The template-assisted zinc cations incorporated SAPO-34 catalysts were characterized by XRD, XRF, N2 adsorption-desorption, XPS, SEM, EDX,NMR, respectively. Enhanced selectivity to ethylene and ratio of ethylene to propylene in MTO reaction are observed over the zinc cations modified SAPO-34 catalysts, due to the facilitated formation of lower methylbenzenes that favour the ethylene gen eration, as well as the increased diffusion hindrance originated from the zinc cations incorporation and the facil让ated generation of aromatics compound. | Jiawei Zhong Jingfeng Han Yingxu Wei Shutao Xu Tantan Sun Xinwen Guo Chunshan Song Zhongmin Liu | 2019 | Journal of Energy Chemistry2019，28，5： | 2 |
| 8 | Microporous polyethersulfone membranes prepared under the combined precipitation conditions with non- solvent additives显示文摘 | Li Jingfeng Xu Zhenliang Yang Hu | 2008 | Polymers for Advanced Technologies2008，19，4： | 1 |
| 9 | 显示文摘 | WEIMING XU JINGFENG YAN NIAZU WU | 2000 | Surface Science2000，470，12： | 1 |
| 10 | Effect of TiO2 nanopartieles on the surface morphology and performance of microporous PES membrane 显示文摘 | Li Jingfeng Xu Zhenliang Yang Hu | 2009 | Appl Surf Sei2009，255，： | 1 |
| 11 | Fabrication and electromechanical properties of microscale 1-3-type piezoelectric composites using (Na,K)NbO3-based Pb-free piezoceramics 显示文摘 | SHEN Zongyang XU Ying LI Jingfeng | 2009 | J Appl Phys2009，105，10： | 1 |
| 12 | Breeding of a New Clubroot-resistant Chinese Cabbage Variety, CCR11242显示文摘In this study,using Japanese radish cytoplasmic sterile line ' Half Green Cabbage'( HGC) and clubroot-resistant South Korean cabbage variety ' CR Cabbage King'( CCK) as experimental materials,self-incompatible DH lines were obtained by microspore culture.Through five generations of backcross,CCR11239,a clubroot-resistant radish cytoplasmic sterile line,was obtained.Through six generations of self-crossing,CCR11240,the maintainer line of CCR11239,was bred.After the cross of CCK and Luchunbai 1( 83- 1) and five generations of self-crossing,self-compatible line CCR11241 was obtained.Through crossing CCR11240 with CCR11241,the new cabbage variety CCR11242 was obtained.After variety comparison test,provincial regional test and production demonstration test,the new variety was registered in 2012.The average yield of CCR11242 reached 60 975 kg / hm2,which was improved by 142% compared with Luchunbai 1( 83- 1).The disease index of CCR11242 was 5.63,which was 88.13 lower than the control,indicating high resistance( HR) to clubroot. | Xuezhong XU Zhimei TIAN Hongli YANG Jingfeng HU Liqin ZHANG Mei LAN Hongju DUAN Jiangming HE | 2016 | Agricultural Biotechnology2016，5，5： | 1 |
| 13 | Insight into the Dual Cycle Mechanism of Methanol-to-OIefins Reaction over SAPO-34 Molecular Sieve by Isotopic Tracer Studies显示文摘Methanol-to-olefins(MTO)reaction is one of the important non-petroleum routes to produce light olefinsover acidic molecular sieves.In this study,the complete reaction course of MTO on SAPO-34 molecular sieve with retained organics evolution from induction period to deactivation period was investigated systematically at different weight hourly space velocities(WHSV)of methanol.By the aid of 12C/3C-methanol isotopic switch experiment,the dual cycle mechanism involving aromatics-based cycle and alkenes-based cycle was evaluated during the whole reac-tion process.The detailed reaction route varied with the evolution of the retained organics in the catalyst at different reaction stages.The aromatics-based cycle and alkenes-based cycle alternately dominate the reaction process.In the efficient reaction period,aromatics-based cvcle is the main reaction mechanism,while in the induction and deactiva-tion periods.the contribution of alkenes-based cvcle mechanism will become more important. | YU Bowen LOU Caiyi ZHANG Wenna XU Shutao HAN Jingfeng YU Zhengxi WEI Yingxu LIU Zhongmin | 2020 | Chemical Research in Chinese Universities2020，36，6： | 1 |
| 14 | Rapid and simultaneous detection of Ureaplasma parvum and Chlamydia trachomatis antibodies based on visual protein microarray using gold nanoparticles and silver enhancement显示文摘 | Jingfeng Tang Zhenghui Xu Li Zhou Hong Qin Yefu Wang Hanhua Wang | 2010 | Diagnostic Microbiology & Infectious Disease2010，，2： | 1 |
| 15 | Application of multi-layered polyurethane foams for flat-walled anechoic linings 显示文摘 | Xu Jingfeng Buchholz J M Fricke F R | 2006 | Applied Acoustics2006，67，5： | 1 |
| 16 | Theoretical analysis of recording light distribution in phase-change near-field recording using solid immersion lens显示文摘 | Liu Jingfeng Chong T C Xu Baoxi | 2000 | Japanese J Appl Phys: Part 12000，39，2： | 1 |
| 17 | Diffusing behavior of MoO3 on Al2O3 and SiO2 thin films显示文摘 | XU Weiming YAN Jingfeng WU Nianzu | 2000 | Surf Sci2000，470，12： | 1 |
| 18 | Optimising Flat-walled Multi-layered Anechoic Linings Using Evolutionary Algorithms显示文摘 | Xu Jingfeng Joseph Nannariello Fergus R Fricke | 2004 | Applied Acoustics2004，65，： | 1 |
| 19 | Optimal risk control and dividend distribution policies for a diffusion model with terminal value显示文摘 | Jingfeng Xu Ming Zhou | 2012 | Mathematical and Computer Modeling2012，5678，： | 1 |
| 20 | Isolated Microspore Culture and Plant Regeneration of Leaf Mustard(Brassica juncea(L.)Czern.et Coss.)显示文摘Isolated microspore culture was carried out with Brassica juncea to study the effects of sterilization methods,culture period of embryos,hormone formula and the concentration of activated carbon on embryogenesis,and the concentration of NAA on induction of roots. The results showed that the embryogenesis on medium subjected to filter sterilization is better than medium subjected to autoclaved sterilization. The embryos cultured for 15 d had the highest plantlet regeneration rate( 52%). NLN-13 liquid medium including 0. 1 mg / L 6-BA and 0. 2 g / L activated carbon significantly improved the plantlet regeneration rate. 1 /2 MS including 0. 3 mg / L NAA had the highest plantlet regeneration rate( 100%). | Hongli YANG Jingfeng HU Xuezhong XU Jiangming HE Shuang SONG 无 | 2016 | Agricultural Biotechnology2016，5，6： | 1 |
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