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| 1 | Construction of a regulable gene therapy vector targeting for hepatocellular carcinoma显示文摘AIM: To construct a gene modified hepatocellular carcinoma (HCC) specific EGFP expression vector regulated by abbreviated cis-acting element of AFP gene.METHODS: The minimal essential DNA segments of AFP gene enhancer and promoter were synthesized through PCR from Genome DNA of HepG2 cells. Gene fragments were then cloned into the multiple cloning site of non-promoter EGFP vector pEGFP-t. Recombinant plasmid was transferred into positive or negative AFP cell lines by means of lipofectamine. The expression of EGFP was tested by fluorescence microscope and flow cytometry. The effect of all-trans retinoic acid (ATRA) on the expression of EGFP was tested in different concentrations.RESULTS: By the methods of restriction digestion and sequence analyses we confirmed that the length, position and orientation of inserted genes of cis-acting element of AFP were all correct. The transcription of EGFP was under the control of AFP cis-acting element. The expressing EGFP can only been detected in AFP producing hepatoma cells.The expression rate of EGFP in G418 screened cell line was 34.9±4.1%. 48 h after adding 1×10-7M retinoic acid, EGFP expression rate was 14.7±3.5%. The activity of AFP gene promoter was significantly suppressed by addition of 1×10-7M retinoic acid (P<0.05, P=0.003, t=6.488).CONCLUSION: This recombinant expression vector can be used as a gene therapy vector for HCC. The expression of tumor killing gene will be confined within the site of tumor and the activity of which can be regulated by retinoic acid. | Shao-YingLu Yan-FangSui Zeng-ShanLi Cheng-EnPan JingYe Wen-YongWang | 2003 | World Journal of Gastroenterology2003，9，4： | 20 |
| 2 | Polar bodies are efficient donors for reconstruction of human embryos for potential mitochondrial replacement therapy显示文摘 | Keliang Wu Cuiqing Zhong Tailai Chen Xiaoyu Zhang Wenrong Tao Jingye Zhang Hongchang Li Han Zhao Jinsong Li Zi-Jiang Chen | 2017 | Cell Research2017，27，8： | 13 |
| 3 | Inhibitory effect of human telomerase antisense oligodeoxyribonucleotides on the growth of gastric cancer cell lines in variant tumor pathological subtype显示文摘AIM: To investigate the inhibitory effect of specialized human telomerase antisense oligodeoxyribonucleotides on the growth of well (MKN-28), moderately (SGC-7901)and poorly (MKN-45) differentiated gastric cancer cell lines under specific conditions and its inhibition mechanism,and to observe the correlation between the growth inhibition ratio and the tumor pathologic subtype of gastric cancer cells.METHODS: Telomerase activity in three gastric cancer cell lines of variant tumor pathologic subtype was determined by modified TRAP assay before and after the specialized human telomerase antisense oligodeoxyribonucleotides were dealt with under specific conditions. Effect of antisense oligomer under specific conditions of the growth and viability of gastric cancer cell lines was explored by using trypan blue dye exclusion assay, and cell apoptosis was detected by cell morphology observation, flow cytometry and TUNEL assay.RESULTS: Telomerase activity was detected in well,moderately and poorly differentiated gastric cancer cell lines (the quantification expression of telomerase activity was 43.7TPG, 56.5TPG, 76.7TPG, respectively).Telomerase activity was controlled to 30.2TPG, 36.3TPG and 35.2TPG for MKN-28, SGC-7901 and MKN-45 cell lines respectively after treatment with human telomerase antisense oligomers at the concentration of 5 μmol/L, and was entirely inhibited at 10 μmol/L, against the template region of telomerase RNA component, whereas no inhibition effect was detected in missense oligomers (P<0.05). After treatment with antisense oligomers at different concentrations under specific conditions for 96 h, significant growth inhibition effects were found in MKN-45 and SGC-7901gastric cancer cell lines (the inhibition ratio was 40.89%and 71.28%), but not in MKN-28 cell lines (15.86%). The ratio of inactive SGC-7901 cells increased according to the prolongation of treatment from 48 to 96 h. Missense oligomers could not lead to the same effect (P<0.05).Apoptosis of SGC-7901 and MKN-45 cells was detected not only by morphology and TUNEL assay but also by flow cytometry. The apoptotic rate reached 33.56% for SGC-7901 cells and 44.75% for MKN-45 cells.CONCLUSION: The viability and proliferation of gastric cancer cells can be inhibited by antisense telomerase oligomers. The growth inhibition of gastric cancer cells is correlated with concentration, time and sequence specialty of antisense oligomers. The inhibition mechanism of antisense human telomerase oligomers depends not only on the sequence specialty but also on the biological characteristics of gastric cancer cell lines. | JingYe Yun-LinWu ShuZhang ZiChen Li-XiaGuo Ruo-YuZhou HongXie | 2005 | World Journal of Gastroenterology2005，11，15： | 9 |
| 4 | oncausal spatial prediction filtering based on an ARMA model显示文摘常规频域预测滤波方法是建立在自回归(autoregressive,AR)模型基础上的,这导致滤波过程中前后假设的不一致,即首先利用源噪声的假设计算误差剖面,却又将其作为可加噪声而从原始剖面中减去来得到有效信号。本文通过建立自回归-滑动平均(autoregres sive/moving-average,ARMA)模型,首先求解非因果预测误差滤波算子,然后利用自反褶积形式投影滤波过程估计可加噪声,进而达到去除随机噪声目的。此过程有效避免了基于AR模型产生的不一致性。在此基础上,将一维ARMA模型扩展到二维空间域,实现了基于二维ARMA模型频域非因果空间预测滤波在三维地震资料随机噪声衰减中的应用。模型试验与实际资料处理表明该方法在很好保留反射信息同时,压制随机噪声更加彻底,明显优于常规频域预测去噪方法。 | Liu Zhipeng Chen Xiaohong Li Jingye | 2009 | Applied Geophysics2009，6，2： | 7 |
| 5 | Clinical significance of serum IGF-I,IGF-II and IGFBP-3 in liver cirrhosis显示文摘AIM: To investigate the relationship between insulin-like growth factor-Ⅰ, -Ⅱ (IGF-Ⅰ and IGF-Ⅱ), IGF-binding protein 3(IGFBP-3) and Child-Pugh score in patients with liver cirrhosis,and to search for potential clinical markers of liver function.METHODS: Forty-four patients with advanced liver cirrhosis of viral origin were divided into 3 groups according to severity of cirrhosis (Child-Pugh score) and 38 healthy subjects served as controls. Serum levels of IGF-Ⅰ, IGF-Ⅱ and IGFBP-3 were measured by immunoradiometric assay.RESULTS: Serum IGF-Ⅰ, IGF-Ⅱ and IGFBP-3 levels were significantly lower in patients with cirrhosis than in controls,and serum concentrations of IGF-Ⅰ, IGF-Ⅱ and IGFBP-3 were associated with the severity of liver dysfunction, and dropped sharply during the progression of liver failure. Among these 3 parameters, serum IGF-Ⅱ was the most sensitive and effective indicator for liver dysfunction. Concentrations of IGF-Ⅰ <30 ng/mL, IGF-Ⅱ <200 ng/mL and IGFBP-3 <6 ng/mL implied a negative prognosis for patients with liver cirrhosis.CONCLUSION: Serum IGF-Ⅰ, IGF-Ⅱ and IGFBP-3 may provide a new dimension in the assessment of liver dysfunction.Combined detection of serum IGF-Ⅰ, IGF-Ⅱ and IGFBP-3 with Child-Pugh score is more effective in predicting prognosis than Child-Pugh score alone. | Yun-LinWu JingYe ShuZhang JieZhong Rong-PingXi | 2004 | World Journal of Gastroenterology2004，10，18： | 7 |
| 6 | Radiation induced graft polymerization of multi-walled carbon nanotubes for superhydrophobic composite membrane preparation显示文摘Highly soluble multi-walled carbon nanotubes(MWNTs) were prepared by radiation-induced free radical graft polymerization of vinyl acetate(VAc) onto pristine MWNT surfaces. High resolution transmission electron microscopy(HR-TEM), Fourier transform infrared(FTIR) spectroscopy, and micro-Raman spectroscopy were used to confirm that poly(vinyl acetate)(PVAc) had been successfully grafted onto the surface of the MWNTs. The effects of experimental parameters on the degree of grafting(DG) of PVAc were also investigated, including adsorbed dose, dose rate, initial monomer concentration, and solvents. The grafted MWNTs(MWNTs-g-PVAc) exhibited good solubility in common organic solvents at high mass fraction. In addition, a superhydrophobic composite membrane could be readily fabricated by vacuum filtration of MWNTs-g-PVAc onto a supporting membrane, as was confirmed by water contact angle testing and visualization by scanning electron microscopy. | Bowu Zhang Siyuan Xie Rongmao Wei Hongjuan Ma Ming Yu Linfan Li Jingye Li | 2016 | Science China Chemistry2016，59，3： | 4 |
| 7 | Imaging algorithm for synthetic aperture interferometric radiometer in near field显示文摘With the benefits of digital IC technology development, the synthetic aperture interferometric radiometer (SAIR) technique is growing fast and expanding to more and more application areas. The near field imaging detection is a potential application which has received increasing demand recently. Because the Fourier imaging theory of the traditional SAIR is based on far-field approximation, it will be invalid for near-field condition. This paper is devoted to establishing a new accurate imaging algorithm for near-field SAIR imaging. Firstly, the visibility function in near field is deduced and the relationship of which to far-field visibility function is analyzed. Then, a numerical method based on pseudo inverse and focal plane approximation is developed. The effectivity of this method is tested with imaging simulation of point source and extended source, and the superiority is also demonstrated by comparing with the existing phase-modified Fourier transform method. At last, the field experiment with one-dimensional SAIR instrument is performed to validate the practical feasibility of this method. | ZHANG Cheng WU Ji LIU Hao YAN JingYe | 2011 | Science China(Technological Sciences)2011，54，8： | 4 |
| 8 | High-selective removal of ultra-low level mercury ions from aqueous solution using oligothymonucleic acid functionalized polyethylene film显示文摘We prepare oligothymonucleic acid (OTA) functionalized polyethylene (PE) film and evaluate its selective removal ability of mercury ions at ultra-low levels in aqueous solution. The selective binding of OTA with mercury ions is confirmed by fluorescence in situ hybridization (FISH). The quantitative results via cold-vapor atomic fluorescence spectrometry (CVAFS) indicate that OTA-functionalized PE film is able to remove mercury ions at the sub-ppb level selectively from aqueous solution, even with the coexistence of other metal ions at concentrations 250-fold or higher than that of mercury. | YU Yang ZHANG BoWu YU Ming DENG Bo LI LinFan FAN ChunHai LI JingYe | 2012 | Science China Chemistry2012，55，10： | 3 |
| 9 | In vitro expansion of human sperm through nuclear transfer显示文摘Dear Editor,Mammalian haploid embryonic stem cells(haESCs)represent an ideal tool for genetic analysis due to the presence of only one set of genetic materials.HaESCs fall into two readily distinguishable groups based on their genome origins:parthenogenetic haESCs(PG-haESCs)that are derived from oocyte-originated parthenogenetic embryos and androgenetic haESCs(AG-haESCs)that are produced through sperm nuclear transfer.Both PG-and AGhaESCs are feasible for delineating genome function at cellular level in vitro.Importantly,AG-haESCs can be used as a sperm replacement and applied to deciphering gene function at organismal level when combined with CRISPR-Cas9 technology.1 However,while PG-haESCs have been generated from both rodent and primates,2-5 AG-haESCs can only be obtained in rodent to date. | Xiaoyu Merlin Zhang Keliang Wu Yuxuan Zheng Han Zhao Junpeng Gao Zhenzhen Hou Meiling Zhang Jiaoyang Liao Jingye Zhang Yuan Gao Yuanyuan Li Lin Li Fuchou Tang Zi-Jiang Chen Jinsong Li | 2020 | Cell Research2020，30，4： | 3 |
| 10 | Purposeless repeated acquisition time-lapse seismic data processing显示文摘在中国,大多数油域是有强壮的异质的大陆人沉积,在一个上,方面使它成为勘探的水库和开发更困难,但是在其它上,方面为在成熟的域里寻找剩余油提供更多的空间。微速摄影的地震水库监视技术是很重要的技术之一定义剩余油分发。根据对的要求和微速摄影的地震水库在中国监视的开发,无目的的重复获得处理的微速摄影的地震数据被学习。四把钥匙在无目的的重复获得走处理的微速摄影的地震数据,包括与相对一致性, rebinning,匹配过滤和差别计算的保存振幅的处理,被联合理论和处理的真实地震数据分析。同时,质量控制在期间真实微速摄影的地震处理被强调。 | Li Jingye Chen Xiaohong Zhao Wei Zhang Yunpeng | 2008 | Petroleum Science2008，5，1： | 2 |
| 11 | MICROWAVE-ASSISTED SURFACE MODIFICATION OF CALCIUM BICARBONATE显示文摘Surface modification of calcium bicarbonate powder with isopropyl triisostearoyl titanate (TTS) by microwave-assisted heating was studied in the present work. The features of microwave treated powder show obvious superiority to those of powder samples treated by traditional surface modification method and of untreated calcium bicarbonate — in suspension turbidity, suction potential, contact angle with water, and mechanical properties of their composites with PVC resin. | JingYe XiaofeiZhang | 2004 | China Particuology2004，2，1： | 2 |
| 12 | Silicon-based on-chip diplexing/triplexing technologies and devices显示文摘Wavelength-division-multiplexing(WDM) transceiver filters are one of the most essential components for realizing the fiber-to-the-home(FTTH) networks. In recent years, silicon photonics have provided a very attractive platform to build ultra-compact photonic integrated devices with CMOS-compatible processes. In this review, we focus on the recent progresses on diplexers/triplexers based on multimode interference couplers(MMI), and directional couplers(DC). The polarization-insensitive devices are also discussed. | Yaocheng SHI Jingye CHEN Hongnan XU | 2018 | Science China(Information Sciences)2018，61，8： | 2 |
| 13 | Molecular architecture of mouse and human pancreatic zymogen granules: protein components and their copy numbers显示文摘A molecular model of pancreatic zymogen granule (ZG) is critical for understanding its functions. We haveextensively characterized the composition and membrane topology of rat ZG proteins. In this study, wereport the development of targeted proteomics approaches to quantify representative mouse and humanZG proteins using LC-SRM and heavy isotope-labeled synthetic peptides. The absolute quantities of mouseRab3D and VAMP8 were determined as 1242 ± 218 and 2039±151 (mean ± SEM) copies per ZG. Thesize distribution and the averaged diameter of ZGs 750 ±23 nm (mean ± SEM) were determined byatomic force microscopy. The absolute quantification of Rab3 D was then validated using semi-quantitativeWestern blotting with purified GST-Rab3D proteins as an internal standard. To extend our proteomicsanalysis to human pancreas, ZGs were purified using human acini obtained from pancreatic islet trans-plantation center. One hundred and eighty human ZG proteins were identified for the first time includingboth the membrane and the content proteins. Furthermore, the copy number per ZG of human Rab3 D andVAMP8 were determined to be 1182 ± 45 and 485 ± 15 (mean ± SEM). The comprehensive proteomicanalyses of mouse and human pancreatic ZGs have the potential to identify species-specific ZG proteins.The determination of protein copy numbers on pancreatic ZGs represents a significant advance towardsbuilding a quantitative molecular model of a prototypical secretory vesicle using targeted proteomicsapproaches. The identification of human ZG proteins lays a foundation for subsequent studies of altered ZGcompositions and secretion in pancreatic diseases. | Jin-sook Lee Joseph A. Caruso Garrett Hubbs Patricia Schnepp Jlames Woods Jingye Fang Chunying Li Kezhong ghang Paul M. Stemmer Bhanu P. Jena Xuequn Chen | 2018 | Biophysics Reports2018，4，2： | 2 |
| 14 | The diagnostic value of apolipoprotein E in malignant pleural effusion associated with non-small cell lung cancer显示文摘 | Yumin Wang Zengqiang Chen Jie Chen Jingye Pan Wenhui Zhang Qinshi Pan Hongyan Ding Xiaomei Lin Xiushu Wen Yangyang Li Qing H. Meng | 2013 | Clinica Chimica Acta2013，，： | 1 |
| 15 | A technique for jam- ming Bi- and multistatic SAR systems 显示文摘 | WANG Wenqin CAI Jingye | 2007 | IEEE Ge- oscience and remote sensing letters2007，4，1： | 1 |
| 16 | Near-space microwave radar remote sensing:potentials and challenge analysis显示文摘 | Wen Qin Wang Jingye Cai Qicong Peng | | 0，，02： | 1 |
| 17 | A technique for jamming bi-and multistatic SAR systems 显示文摘 | WANG Wenqin CAI Jingye | 2007 | IEEE Geoscience and Remote Sensing Letters2007，，： | 1 |
| 18 | Comparative study on traumatic effect and L-selectin change made by two blooddrawing modes in rat显示文摘Objective: To compare carotid catheters blooddrawing and tail-cut blooddrawing in traumatizing rats and changing their internal L-selectin. Methods: Two ways of bloodletting were selected to produce hemorrhagic shock models. Monoclonal antibody sign and flow cytometer were used to test neutrophil L-selectin dynamic expression in rats. Results: No remarkable differences were shown among different time points in neutrophil L-selectin expressing amount (average fluorescence index)(P>0.05). Compared with the normal control group, all cell surface expressions in empirical control group increased. Cell surface expressions reached the summit at 3 hours and kept the high level at 4-5 hours. A progressive increase of the mRNA expression peaked at 5 hours; the expressions in both groups of carotid catheters blooddrawing increased compared with normal control group, and remained stable after 3 hours compared with the Tail-cut Blooddrawing Group. Conclusion: L-selectin expressions changed little in tail-cut blooddrawing rats, while carotid catheters resulted moderate or sever trauma. Therefore, it was suggested that it should not be set as hemorrhagic shock control model. As neutrophil L-selection expressions increased after trauma, the adhesion of leucocytes to the venule wall may be beneficial to the healing of wound and enhance the ability of anti-infection. The immediate increasing of neutrophil surface L-selectin expressions in this study was likely due to the direct release of L-selectin from cytoplasm granules that do not depend on protein synthesis. | Jingye Pan Dakai Chen Zhijian Shen Lei Chen Weixing Zhu | 2005 | Journal of Nanjing Medical University2005，19，4： | 1 |
| 19 | Pre-Irradiation Induced Grafting of Styrene Into Crosslinked and Non-Crosslinked Polytetrafluoroethylene Films for Polymer Electrolyte Fuel Cell Applications Ⅰ : Influence of Styrene Grafting Conditions显示文摘 | LI Jingye Kohei S Shogo I | 2004 | Eur Polym J2004，40，： | 1 |
| 20 | Active control ofstructurally radiated sound from an elastic cylindrical shell显示文摘 | JinGY LiuXL LiuZG | | 0，，： | 1 |
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