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| 1 | Tribenuron-Methyl Induces Male Sterility through Anther-Specific Inhibition of Acetolactate Synthase Leading to Autophagic Cell Death显示文摘Tribenuron 甲基(TM ) 是禁止分支链的强大的 sulfonylurea 除草剂由指向 acetolactate synthase (ALS ) 的催化子单元(CSR1 ) 的氨基酸(BCAA ) 生合成。由在低剂量的 TM 的叶的喷洒的男绝育的选择正式就职广泛地在油菜籽(Brassica 马来麝) 为混合种子生产被使用了;然而,内在的机制仍然保持未知。这里,我们 reportgreater TM 累积和在花药的 BCAA 饥饿比在在 TM 申请以后的叶子和茎。csr1-1D (aCSR1 异种) 的组成或花药特定的表示消除了花药选择的 ALS 抑制并且在油菜籽和 Arabidopsis 颠倒了导致 TM 的男无菌的显型。TM 涂抹茎实验的结果,在叶肉或韧皮部由 TM 新陈代谢基因 Bel 的指向的表示在导致 TM 的男绝育的花药和回复与很少 TM 累积的观察结合了,建议叶水花的 TM 通过叶肉和韧皮部主要对花药极运输。显微镜学和 immunoblotting 揭示了那 autophagy,在房间死亡期间导致的一个体积降级过程,在导致 TM 的男无菌的花药并且由被提高花药特定 ALS 击倒。而且,导致 TM 的花粉流产被 autophagy 禁止者 3 麻省显著地禁止。这些数据最终建议 TM 对花药极运输,导致经由花药特定的 ALS 抑制的 BCAA 饥饿并且,,在花药的 autophagic 房间死亡。 | Lun Zhao Xue Jing Li Chen Yingjun Liu Yanan Su Tingting Liu Changbin Gao Bin Yi Jing Wen Chaozhi Ma Jinxing Tu Jitao Zou Tingdong Fu Jinxiong Shen | 2015 | Molecular Plant2015，8，12： | 7 |
| 2 | The heterogeneity of islet autoantibodies and the progression of islet failure in type 1 diabetic patients显示文摘Type 1 diabetes mellitus is heterogeneous in many facets. The patients suffered from type 1 diabetes present several levels of islet function as well as variable number and type of islet-specific autoantibodies. This study was to investigate prevalence and heterogeneity of the islet autoantibodies and clinical phenotypes of type 1 diabetes mellitus; and also discussed the process of islet failure and its risk factors in Chinese type 1 diabetic patients. A total of 1,291 type 1 diabetic patients were enrolled in this study. Demographic information was collected. Laboratory tests including mixed-meal tolerance test, human leukocyte antigen alleles, hemoglobin A1 c, lipids, thyroid function and islet autoantibodies were conducted. The frequency of islet-specific autoantibody in newly diagnosed T1 DM patients(duration shorter than half year) was 73% in East China. According to binary logistic regressions, autoantibody positivity, longer duration and lower Body Mass Index were the risk factors of islet failure. As the disease developed, autoantibodies against glutamic acid decarboxylase declined as well as the other two autoantibodies against zinc transporter 8 and islet antigen 2. The decrease of autoantibodies was positively correlated with aggressive beta cell destruction. Autoantibodies can facilitate the identification of classic T1 DM from other subtypes and predict the progression of islet failure. As there were obvious heterogeneity in autoantibodies and clinical manifestation in different phenotypes of the disease, we should take more factors into consideration when identifying type 1 diabetes mellitus. | Jin Liu Lingling Bian Li Ji Yang Chen Heng Chen Yong Gu Bingqin Ma Wei Gu Xinyu Xu Yun Shi Jian Wang Dalong Zhu Zilin Sun Jianhua Ma Hui Jin Xing Shi Heng Miao Bing Xin Yan Zhu Zhenwen Zhang Ruifang Bu Lan Xu Guangde Shi Wei Tang Wei Li Dongmei Zhou Jun Liang Xingbo Cheng Bimin Shi Jixiang Dong Ji Hu Chen Fang Shao Zhong Weinan Yu Weiping Lu Chenguang Wu Li Qian Jiancheng Yu Jialin Gao Xiaoqiang Fei Qingqing Zhang Xueqin Wang Shiwei Cui Jinluo Cheng Ning Xu Guofeng Wang Guoqing Han Chunrong Xu Yun Xie Minmin An Wei Zhang Zhixiao Wang Yun Cai Qi Fu Yu Fu Shuai Zheng Fan Yang Qingfang Hu Hao Dai Yu Jin Zheng Zhang Kuanfeng Xu Yifan Li Jie Shen Hongwen Zhou Wei He Xuqin Zheng Xiao Han Liping Yu Jinxiong She Mei Zhang Tao Yang | 2016 | Science China(Life Sciences)2016，59，9： | 5 |
| 3 | The Brassica napus GATA transcription factor BnA5.ZML1 is a stigma compatibility factor显示文摘Self-incompatibility(SI)is a genetic mechanism that rejects self-pollen and thus prevents inbreeding in some hermaphroditic angiosperms.In the Brassicaceae,SI involves a pollen-stigma recognition system controlled by a single locus known as the S locus,which consists of two highly polymorphic genes that encode S-locus cysteine-rich protein(SCR)and S-receptor kinase(SRK).When self-pollen lands on the stigma,the S-haplotype-specific interaction between SCR and SRK triggers SI.Here,we show that the GATA transcription factor BnA5.ZML1 suppresses SI responses in Brassica napus and is induced after compatible pollination.The loss-of-function mutant bna5.zml1 displays reduced self-compatibility.In contrast,overexpression of BnA5.ZML1 in self-incompatible stigmas leads to a partial breakdown of SI responses,suggesting that BnA5.ZML1 is a stigmatic compatibility factor.Furthermore,the expression levels of SRK and ARC1 are up-regulated in bna5.zml1 mutants,and they are down-regulated in BnA5.ZML1 overexpressing lines.SRK affects the cellular localization of BnA5.ZML1 through direct protein–protein interaction.Overall,our findings highlight the fundamental role of BnA5.ZML1 in SI responses in B.napus,establishing a direct interaction between BnA5.ZML1 and SRK in this process. | Zhiqiang Duan Yatao Zhang Jinxing Tu Jinxiong Shen Bin Yi Tingdong Fu Cheng Dai Chaozhi Ma | 2020 | Journal of Integrative Plant Biology2020，62，8： | 2 |
| 4 | Genome-wide prediction for hybrids between parents with distinguished difference on exotic introgressions in Brassica napus显示文摘Extensive exotic introgression could significantly enlarge the genetic distance of hybrid parental populations to promote strong heterosis.The goal of this study was to investigate whether genome-wide prediction can support pre-breeding in populations with exotic introgressions.We evaluated seed yield,seed yield related traits and seed quality traits of 363 hybrids of Brassica napus (AACC) derived from two parental populations divergent on massive exotic introgression of related species in three environments.The hybrids presented strong heterosis on seed yield,which was much higher than other investigated traits.Five genomic best linear unbiased prediction models considering the exotic introgression and different marker effects (additive,dominance,and epistatic effects) were constructed to test the prediction ability for different traits of the hybrids.The analysis showed that the trait complexity,exotic introgression,genetic relationship between the training set and testing set,training set size,and environments affected the prediction ability.The models with best prediction ability for different traits varied.However,relatively high prediction ability (e.g.,0.728 for seed yield) was also observed when the simplest models were used,excluding the effects of the special exotic introgression and epistasis effect by5-fold cross validation,which would simplify the prediction for the trait with complex architecture for hybrids with exotic introgression.The results provide novel insights and strategies for genome-wide prediction of hybrids between genetically distinct parent groups with exotic introgressions. | Dandan Hu Yusheng Zhao Jinxiong Shen Xiangxiang He Yikai Zhang Yong Jiang Rod Snowdon Jinling Meng Jochen C.Reif Jun Zou | 2021 | The Crop Journal2021，9，5： | 2 |
| 5 | An in planta haploid induction system in Brassica napus显示文摘Doubled haploid technology is widely used to accelerate plant breeding,but its use in the important oilseed crop Brassica napus L.is limited because B.napus haploids could only be obtained through in vitro anther or microspore cultures.Recently,maize(Zea mays)lines containing mutations in Domain of unknown function 679 membrane protein(DMP)were used as haploid inducer lines.This new haploid induction mechanism has been extended to several other plants,including the dicots Arabidopsis thaliana,tomato(Solanum lycopersicum),and tobacco(Nicotiana tabacum).Here,we knocked out four BnaDMP genes in the B.napus cultivar Westar using a clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9 vector with an enhanced green fluorescent protein expression cassette.Plants with DMP mutations in B.napus in the T_(0),T_(1),and T_(2) generations exhibited a haploid induction rate up to 2.53%.These results suggest that targeting BnaDMP could be useful for haploid induction in B.napus. | Yifan Li Dan Li Qing Xiao Huadong Wang Jing Wen Jinxing Tu Jinxiong Shen Tingdong Fu Bin Yi | 2022 | Journal of Integrative Plant Biology2022，64，6： | 2 |
| 6 | Bn.YCO affects chloroplast development in Brassica napus L.显示文摘Mature chloroplasts,as the main sites of photosynthesis,are essential for seedling growth in higher plants.Loss of function of genes involved in chloroplast development changes plant phenotype.We obtained a YELLOW COTYLEDON (YCO) mutant in rapeseed (Brassica napus L.) using CRISPR-Cas9.Bn.YCO,a gene of unknown function,has two homologous copies (Bna A01.YCO and Bna C01.YCO) in B.napus.Homozygous mutation of these two homologs resulted in yellow cotyledons and chlorotic true leaves.Transmission electron microscopy revealed that the formation of thylakoid membranes was inhibited in yellow cotyledons.Sequence similarity search revealed that YCO was conserved in different species,and a subcellular location assay verified that Bn.YCO was located in the chloroplast.Bn.YCO was expressed in multiple tissues,most highly in cotyledons.Knockout of Bn.YCO blocked the transcription of plastid genes,especially those of photosystem genes transcribed by plastid-encoded polymerase.Transcriptome sequencing showed that the majority of genes involved in ribosome assembly and photosynthesis were down-regulated in Bn.yco mutants.These results suggested that loss of function of Bn.YCO affected plastid gene transcription,which influenced chloroplast biogenesis in rapeseed seedlings. | Tingting Liu Baolong Tao Hanfei Wu Jing Wen Bin Yi Chaozhi Ma Jinxing Tu Tingdong Fu Lixia Zhu Jinxiong Shen | 2021 | The Crop Journal2021，9，5： | 1 |
| 7 | Mapping of BnMs4 and BnRf to a common microsyntenic region of Arabidopsis thaliana chromosome 3 using intron polymorphism markers显示文摘 | Shengqian Xia Ling Cheng Feng Zu Xiaoling Dun Zhengfu Zhou Bin Yi Jing Wen Chaozhi Ma Jinxiong Shen Jinxing Tu Tingdong Fu | 2012 | Theoretical and Applied Genetics2012，，7： | 1 |
| 8 | A separation defect of tapetum cells and microspore mother cells results in male sterility in Brassica napus: the role of abscisic acid in early anther development显示文摘 | Yun Zhu Xiaoling Dun Zhengfu Zhou Shengqian Xia Bin Yi Jing Wen Jinxiong Shen Chaozhi Ma Jinxing Tu Tingdong Fu | 2010 | Plant Molecular Biology (-)2010，，1： | 1 |
| 9 | Genetic characterization of a new cytoplasmic male sterility system (hau) in Brassica juncea and its transfer to B. napus显示文摘 | Zhengjie Wan Bing Jing Jinxing Tu Caozhi Ma Jinxiong Shen Bin Yi Jing Wen Tao Huang Xianjun Wang Tingdong Fu | 2008 | Theoretical and Applied Genetics2008，，3： | 1 |
| 10 | A separation defect of tapetum cells and microspore mother cells results in male sterility in Brassica napus: the role of abscisic acid in early anther development显示文摘 | Yun Zhu Xiaoling Dun Zhengfu Zhou Shengqian Xia Bin Yi Jing Wen Jinxiong Shen Chaozhi Ma Jinxing Tu Tingdong Fu | 2010 | Plant Molecular Biology (-)2010，，1： | 1 |
| 11 | Asymmetric epigenome maps of subgenomes reveal imbalanced transcription and distinct evolutionary trends in Brassica napus显示文摘The complexity of the epigenome landscape and transcriptional regulation is significantly increased during plant polyploidization,which drives genome evolution and contributes to the increased adaptability to diverse environments.However,a comprehensive epigenome map of Brassica napus is still unavailable.In this study,we performed integrative analysis of five histone modifications,RNA polymerase Ⅱ CCU-pancy,DNA methylation,and transcriptomes in two B.napus lines(2063A and B409),and established global maps of regulatory elements,chromatin states,and their dynamics for the whole genome(including the An and Cn subgenomes)in four tissue types(young leaf,flower bud,silique,and root)of these two lines.Approximately 65.8% of the genome was annotated with different epigenomic signals.Compared with the Cn subgenome,the An subgenome possesses a higher level of active epigenetic marks and lower level of repressive epigenetic marks.Genes from subgenome-unique regions contribute to the major differences between the An and Cn subgenomes.Asymmetric histone modifications between homeologous gene pairs reflect their biased expression patterns.We identified a novel bivalent chromatin state(with H3K4me1 and H3K27me3)in B.napus that is associated with tissue-specific gene expression.Furthermore,we observed that different types of duplicated genes have discrepant patterns of histone modification and DNA methylation levels.Collectively,our findings provide a valuable epigenetic resource for allopolyploid plants. | Qing Zhang Pengpeng Guan Lun Zhao Meng Ma Liang Xie Yue Li Ruiqin Zheng Weizhi Ouyang Shunyao Wang Hongmeijuan Li Ying Zhang Yong Peng Zhilin Cao Wei Zhang Qin Xiao Yuanling Xiao Tingdong Fu Guoliang Li Xingwang Li Jinxiong Shen | 2021 | Molecular Plant2021，14，4： | 1 |
| 12 | A review: status and prospect of heterosis prediction in rapeseed(Brassica napus L.)显示文摘Heterosis has been widely exploited and utilized in rapeseed breeding. Reliable and precise prediction techniques of heterosis contributed in accelerating the crossbreeding and reducing the cost of large-scale field evaluation. Combined with our work in last decades, this review summarized the progress on heterosis prediction of rapeseed (Brassica napus L.), compared the efficiency of different methods, and proposed its prospect. | Lifang Wang Fang Zhang Tingdong Fu Jinxiong Shen | 2017 | Oil Crop Science2017，2，3： | 0 |
| 13 | Comparative phosphoproteomic analysis of compatible and incompatible pollination in Brassica napus L.显示文摘Self-incompatibility(SI)promotes outbreeding and prevents self-fertilization to promote genetic diversity in angiosperms.Several studies have been carried to investigate SI signaling in plants;however,protein phosphorylation and dephosphorylation in the fine-tuning of the SI response remain insufficiently understood.Here,we performed a phosphoproteomic analysis to identify the phosphoproteins in the stigma of self-compatible‘Westar’and self-incompatible‘W-3’Brassica napus lines.A total of 4109 phosphopeptides representing 1978 unique protein groups were identified.Moreover,405 and 248 phosphoproteins were significantly changed in response to SI and self-compatibility,respectively.Casein kinaseⅡ(CKⅡ)phosphorylation motifs were enriched in self-incompatible response and identified 127 times in 827 dominant SI phosphorylation residues.Functional annotation of the identified phosphoproteins revealed the major roles of these phosphoproteins in plant-pathogen interactions,cell wall modification,mRNA surveillance,RNA degradation,and plant hormone signal transduction.In particular,levels of homolog proteins ABF3,BKI1,BZR2/BSE1,and EIN2 were significantly increased in pistils pollinated with incompatible pollens.Abscisic acid and ethephon treatment partially inhibited seed set,while brassinolide promoted pollen germination and tube growth in SI response.Collectively,our results provided an overview of protein phosphorylation during compatible/incompatible pollination,which may be a potential component of B.napus SI responses. | Zhiqiang Duan Shengwei Dou Zhiquan Liu Bing Li Bin Yi Jinxiong Shen Jinxing Tu Tingdong Fu Cheng Dai Chaozhi Ma | 2020 | Acta Biochimica et Biophysica Sinica2020，52，4： | 0 |
| 14 | Functional genomics of Brassica napus:Progresses,challenges,and perspectives显示文摘Brassica napus,commonly known as rapeseed or canola,is a major oil crop contributing over 13%to the stable supply of edible vegetable oil worldwide.Identification and understanding the gene functions in the B.napus genome is crucial for genomic breeding.A group of genes controlling agronomic traits have been successfully cloned through functional genomics studies in B.napus.In this review,we present an overview of the progress made in the functional genomics of B.napus,including the availability of germplasm resources,omics databases and cloned functional genes.Based on the current progress,we also highlight the main challenges and perspectives in this field.The advances in the functional genomics of B.napus contribute to a better understanding of the genetic basis underlying the complex agronomic traits in B.napus and will expedite the breeding of high quality,high resistance and high yield in B.napus varieties. | Zengdong Tan Xu Han Cheng Dai Shaoping Lu Hanzi He Xuan Yao Peng Chen Chao Yang Lun Zhao Qing-Yong Yang Jun Zou Jing Wen Dengfeng Hong Chao Liu Xianhong Ge Chuchuan Fan Bing Yi Chunyu Zhang Chaozhi Ma Kede Liu Jinxiong Shen Jinxing Tu Guangsheng Yang Tingdong Fu Liang Guo Hu Zhao | 2024 | Journal of Integrative Plant Biology2024，66，3： | 0 |
| 15 | Bna A02.YTG1,encoding a tetratricopeptide repeat protein,is required for early chloroplast biogenesis in Brassica napus显示文摘Chloroplasts are essential for plant growth and development,as they play a key role in photosynthesis.The chloroplast biogenesis process is complex and its regulatory mechanism remains elusive.We characterized a spontaneous Brassica napus(rapeseed)mutant,ytg,that showed a delayed greening phenotype in all green organs and retarded growth.We identified Bna A02.YTG1 encoding a chloroplastlocalized tetratricopeptide repeat protein widely expressed in rapeseed tissues.We speculated that the ytg phenotype was caused by the deletion of Bna A02.YTG1 based on sequence comparison of 4608(with normal green leaves,isolated from the elite Chinese rapeseed cultivar ZS11)and ytg combined with transcriptome data and CRISPR/Cas9 gene editing results.The homologous gene(Bna C02.YTG1)restored the phenotype of the mutant.Bna A02.YTG1 interacted with MORF2,MORF8,and OZ1.RNA editing of the ndh D-2,ndh F-290,pet L-5,and ndh G-50 plastid transcripts was affected in ytg.These findings suggested that Bna A02.YTG1 participates in RNA editing events.We predicted 29 RNA editing sites in the chloroplast of Brassica napus by comparison with the Arabidopsis chloroplast genome.We conclude that Bna A02.YTG1 affects the posttranscriptional regulation of plastid gene expression and suggest that a tetratricopeptide repeat protein is involved in the chloroplast RNA editing in rapeseed. | Haiyan Zhang Xiaoting Li Yebitao Yang Kaining Hu Xianming Zhou Jing Wen Bin Yi Jinxiong Shen Chaozhi Ma Tingdong Fu Jinxing Tu | 2022 | The Crop Journal2022，10，3： | 0 |
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