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| 1 | Myeloid-specific targeting of Notch ameliorates murine renal fibrosis via reduced infiltration and activation of bone marrowderived macrophage显示文摘Macrophages play critical roles in renal fibrosis.However,macrophages exhibit ontogenic and functional heterogeneities,and which population of macrophages contributes to renal fibrosis and the underlying mechanisms remain unclear.In this study,we genetically targeted Notch signaling by disrupting the transcription factor recombination signal binding protein-JK(RBP-J),to reveal its role in regulation of macrophages during the unilateral ureteral obstruction(UUO)-induced murine renal fibrosis.Myeloid-specific disruption of RBP-J attenuated renal fibrosis with reduced extracellular matrix deposition and myofibroblast activation,as well as attenuated epithelial-mesenchymal transition,likely owing to the reduced expression of TGF-β.Mean while,RBP-J deletion significantly hampered macrophage infiltration and activation in fibrotic kidney,although their proliferation appeared unaltered.By using macrophage clearance experiment,we found that kidney resident macrophages made negligible contribution,but bone marrow(BM)-derived macrophages played a major role in renal fibrogenesis.Further mechanistic analyses showed that Notch blockade reduced monocyte emigration from BM by down-regulating CCR2 expression.Finally,we found that myeloid-specific Notch activation aggravated renal fibrosis,which was mediated by CCR2^+macrophages infiltration.In summary,our data have unveiled that myeloid-specific targeting of Notch could ameliorate renal fibrosis by regulating BM-derived macrophages recruitment and activation,providing a novel strategy for intervention of this disease. | Yali Jiang Yuanyuan Wang Pengfei Ma Dongjie An Junlong Zhao Shiqian Liang Yuchen Ye Yingying Lu Peng Zhang Xiaowei Liu Hua Han Hongyan Qin | 2019 | Protein & Cell2019，10，3： | 10 |
| 2 | The radioactive abnormality characteristics of typical regions in Ordos Basin and its geological implications显示文摘There are many results of single mineral enrichment characteristic, such as oil, gas, coal and uranium, but little is known about the synergistic research of these important minerals and the study of uranium enrichment features in the deep basin. So, the study on the paragenesis regularity and coexisting relation of many minerals in the basin will promote the integrated forecast and cooperative exploitation of the basin. Based on the plentiful logging data and geological data, this paper studies the distributing feature of higher Gamma abnormality. The analysis on 33 core samples' test results indicates that the increasing of Gamma abnormality is due to the increasing of the uranium element, and the enrichment of uranium is a result of the activation and conglomeration of uranium. On the basis of the recognization of radioactive abnormality and the study about the reality of oil,gas,coal or uranium coexisting in a basin and its mechanism, the paper shows that there is a certain mutual promotion in oil, gas, coal and uranium in the basin, which provides an important theory basis for cooperative exploitation of energy resources. | TAN ChengQian LIU ChiYang ZHAO JunLong ZHANG RongRong | 2007 | Science China Earth Sciences2007，50，z2： | 6 |
| 3 | Vaccination against Schistosoma japonicum Infection by DNA Vaccine Encoding Sj22.7 Antigen显示文摘在鼠标的效果, recombinant 原生质标志 pcDNA3-Sj22.7 与调停 liposome 的方法习惯于 transfectHeLa 房间, Sj22.7 mRNA 和蛋白质的表达式用反向的抄写聚合酶链反应,钠 dodecylsulfate-polyacrylamide 凝胶电泳和蛋白质印迹被检验。然后, pcDNA3-Sj22.7 的能力在鼠标的种痘以后根据蠕虫减小率和鸡蛋减小率被分析免于 Schistosomajaponicum 挑战感染。特定的 IgG 抗体和 T 淋巴细胞增长反应的浆液层次也是坚定的。在挑战感染以后,驾驶 Sj22.7 的干扰素(IFN )-gamma andinterleukin (IL )-4 也被确定。结果证明 pcDNA3-Sj22.7 能表示 Sj22.7 mRNAand 在 vitro.Immunization 的蛋白质导致了29.70%的蠕虫减小率;鸡蛋减小率of47.25%(肝)并且51.73%(肠),并且25.90%的鸡蛋减小率(鸡蛋每女性),在 连接group.Enzyme 的免疫吸着剂试金和蛋白质印迹分析显示了的 pcDNA3-Sj22.7 建议重要反肥沃的正式就职那使对从与 pcDNA3-Sj22.7 使免疫的鼠标的 Sj22.7.Tlymphocytes 的产生特定的 IgG 显示出的鼠标免疫怒气房间的一种重要增长反应 torSj22.7.The 文化证明 IFN-gamma 的那分泌物增加了但是 IL-4decreased.The 结果显示由 pcDNA3-Sj22.7 的 DNA 种痘对足够对 S.japonicum 感染得到保护的免疫的重要层次。DNA 疫苗能导致重要细胞、体液的有免疫力的反应,并且显示占优势的 T 助手房间类型 1 类型免疫者回答,它在鼠标对挑战感染贡献保护的免疫。 | Gan DAI Shiping WANG Junlong YU Shaorui XU Xianchu PENG Zhuo HE Xueqin LIU Songhua ZHOU and Fen LIU Institute of Schistosomiasis Research.Xiangya School of Medicine,Central South University,Changsha 410078 China | 2007 | Acta Biochimica et Biophysica Sinica2007，39，1： | 5 |
| 4 | Terrestrial input and nutrient change reflected by sediment records of the Changjiang River Estuary in recent 80 years显示文摘A variety of environmental problems have been observed in the Changjiang River Estuary and adjacent coastal area, including eutrophication, harmful algal blooms(HABs), and hypoxia in recent decades. Application of sedimentary biogenic element indicators on the study of paleoenvironment can reconstruct environmental evolution history of waters. Two 210Pb-dated cores were collected from the Changjiang River Estuary(S3) and adjacent coastal area(Z13), and total organic carbon(TOC), total nitrogen(TN), biogenic silicon(BSi), total phosphorus(TP) and phosphorus(P) species were analyzed. Three stages of environmental changes are deduced by the nutrient sedimentary records. First, nutrient concentration increased rapidly since the 1950 s, which attributed to agriculture development and overused chemical fertilizers. Second, nutrient concentration kept high and primary production began to promote during the 1960 s to 1980 s, while diatom abundance and proportion began to decline since the 1970 s, accompanied by reduced 23 Si O- concentration and flux from the river. Third, due to several dams and bridges constructed, river runoff and coastal hydrodynamic conditions reduced to a certain extent since the 1990 s, which aggravated the unbalance in nutrient structure. Multi-nutrient proxies in sediment can reflect the natural environm-ental changes as well as influence of human activities. | LI Junlong ZHENG Binghui HU Xupeng WANG Yiming DING Ye LIU Fang | 2015 | Acta Oceanologica Sinica2015，34，2： | 4 |
| 5 | Cloning, expression and protective immunity evalua-tion of the full-length cDNA encoding succinate dehydrogenase iron-sulfur protein of Schistosoma japonicum显示文摘1071-bp fragment was obtained from the Schistosoma japonicum (Chinese strain) adult cDNA library after the 3′ and 5′ ends of the incomplete expression sequence tag (EST) of succinate dehydrogenase iron-sulfur protein of Schistosoma japonicum (SjSDISP) were amplified by the anchored PCR with 2 pairs of primers designed according to the EST of SjSDISP and the sequence of multiclone sites of the library vector. Sequence analysis indicated that the fragment was a full-length cDNA with a complete open reading frame (ORF), encoding 278 amino acid residues. The fragment was cloned into prokary- otic expression vector pQE30, and subsequently sequenced and expressed in Escherichia coli. SDS-PAGE and Western-blot analyses showed that the recombinant protein was about 32 kD and could be recognized by the polyclonal antisera from rabbits immunized with Schistosoma japonicum adult worm antigen. Compared with the FCA controls, mice vaccinated with rSjSDISP (test) or rSjGST (posi- tive control) all revealed high levels of specific antibody and significant reduction in worm burden, liver eggs per gram (LEPG), fecal eggs per gram (FEPG) and intrauterine eggs. These results suggest that SjSDISP may be a novel and partially protective vaccine candidate against schistosomiasis. In contrast to the worm burden reduction rate, the higher degree of egg reduction rate in the test group also sug- gested that SjSDISP vaccine may primarily play a role in anti-embryonation or anti-fecundity immunity. | YU JunLong, WANG ShiPing, LI WenKai, DAI Gan, XU ShaoRui, HE Zhuo, PENG XianChu, ZHOU SongHua & LIU XueQin Department of Parasitology, Xiangya School of Medicine, Central South University, Changsha 410078, China | 2007 | Science China(Life Sciences)2007，50，2： | 4 |
| 6 | Performance of overlapped shield tunneling through an integrated physical model tests,numerical simulations and real-time field monitoring显示文摘In this work,deformations and internal forces of an existing tunnel subjected to a closely overlapped shield tunneling are monitored and analyzed using a series of physical model experiments and numerical simulations.Effects of different excavation sequences and speeds are explicitly considered in the analysis.The results of the physical model experiments show that the bottom-up tunneling procedure is better than the top-down tunneling procedure.The incurred deformations and internal forces of the existing tunnel increase with the excavation speed and the range of influence areas also increase accordingly.For construction process control,real-time monitoring of the power tunnel is used.The monitoring processes feature full automation,adjustable frequency,real-time monitor and dynamic feedback,which are used to guide the construction to achieve micro-disturbance control.In accordance with the situation of crossing construction,a numerical study on the performance of power tunnel is carried out.Construction control measures are given for the undercrossing construction,which helps to accomplish the desired result and meet protection requirements of the existing tunnel structure.Finally,monitoring data and numerical results are compared,and the displacement and joint fracture change models in the power tunnel subject to the overlapped shield tunnel construction are analyzed. | Junlong Yang Chen Liu Qiushi Chen Xiongyao Xie | 2017 | Underground Space2017，2，1： | 3 |
| 7 | Structural variation during dog domestication: insights from gray wolf and dhole genomes显示文摘Several processes like phenotypic evolution, disease susceptibility and environmental adaptations, which fashion the domestication of animals, are largely attributable to structural variations(SVs) in the genome.Here, we present high-quality draft genomes of the gray wolf(Canis lupus) and dhole(Cuon alpinus) with scaffold N50 of 6.04 Mb and 3.96 Mb, respectively. Sequence alignment comprising genomes of three canid species reveals SVs specific to the dog, particularly 16 315 insertions, 2565 deletions, 443 repeats, 16 inversions and 15 translocations. Functional annotation of the dog SVs associated with genes indicates their enrichments in energy metabolisms, neurological processes and immune systems. Interestingly, we identify and verify at population level an insertion fully covering a copy of the AKR1 B1(Aldo-Keto Reductase Family 1 Member B) transcript. Transcriptome analysis reveals a high level of expression of the new AKR1 B1 copy in the small intestine and liver, implying an increase in de novo fatty acid synthesis and antioxidant ability in dog compared to gray wolf, likely in response to dietary shifts during the agricultural revolution. For the first time, we report a comprehensive analysis of the evolutionary dynamics of SVs during the domestication step of dogs. Our findings demonstrate that retroposition can birth new genes to facilitate domestication, and affirm the importance of large-scale genomic variants in domestication studies. | Guo-Dong Wang Xiu-Juan Shao Bing Bai Junlong Wang Xiaobo Wang Xue Cao Yan-Hu Liu Xuan Wang Ting-Ting Yin Shao-Jie Zhang Yan Lu Zechong Wang Lu Wang Wenming Zhao Bing Zhang Jue Ruan Ya-Ping Zhang | 2019 | National Science Review2019，6，1： | 3 |
| 8 | Classification of estuaries in China based on eutrophication susceptibility to nutrient load显示文摘Recently, environmental pressures along coasts have increased substantially. Classification of estuaries according to their susceptibility to eutrophication nutrient load is a useful method to determine priority management objects and to enforce control measures. Using historical monitoring data from 2007 to 2012, from 65 estuaries, including 101 estuarine monitoring sections and 260 coastal monitoring stations, a nutrient-driven phytoplankton dynamic model was developed based on the relationship among phytoplankton biomass, Total Nitrogen(TN) load and physical features of estuaries. The ecological filter effect of estuaries was quantified by introducing conversion efficiency parameter values into the model. Markov Chain Monte Carlo algorithm of Bayesian inference was then employed to estimate parameters in the model. The developed model fitted well to the observed chlorophyll, primary production, grazing, and sinking rates. The analysis suggests that an estuary with Q/V(the ratio of river flow to estuarine volume) greater than 2.0 per year and ε(conversion efficiency ratio) less than 1.0 g C/g N can be classified as less susceptible to TN load, Q/V between 0.7 to 2.0 per year and ε between 1.0 to 3.0 g C/g N as moderately susceptible, and ε greater than 3.0 g C/g N as very susceptible. The estuaries with Q/V less than 0.7 per year vary greatly in their susceptibility. The estuaries with high and moderate susceptibility accounted for 67% of all the analyzed estuaries. They have relatively high eutrophication risks and should be the focus of environmental supervision and pollution prevention. | LI JunLong ZHENG BingHui LIU Yong WU Zhen LIU Fang SHAO JunBo HU XuPeng | 2015 | Science China Earth Sciences2015，58，6： | 2 |
| 9 | Synthesis of novel hierarchical porous polymers with a nanowire-interconnected network structure from core-shell polymer nanoobjects显示文摘Design and fabrication of the micro/nanostructures of the network units is a critical issue for porous nanonetwork structured materials. Significant progress has been attained in construction of the network units with zero-dimensional spherical shapes.However, owing to the limitations of synthetic methods, construction of porous building blocks in one dimension featuring high aspect ratios for porous nanonetwork structured polymer(PNSP) remains largely unexplored. Here we present the successful design and preparation of PNSP with a novel type of one-dimensional network unit, i.e., microporous heterogeneous nanowire. Well-defined core-shell polymer nanoobjects prepared from a gelable block copolymer, poly(3-(triethoxysilyl)propyl methacrylate)-block-polystyrene are employed as building blocks, and facilely transformed into PNSP via hypercrosslinking of polystyrene shell. The as-prepared PNSP exhibits unique three-dimensional hierarchical nanonetwork morphologies with large surface area. These findings could provide a new avenue for fabrication of unique well-defined PNSP, and thus generate valuable breakthroughs in many applications. | yeru liang yingbo ruan junlong huang bo peng chen-yang liu ruowen fu mingqiu zhang yongming chen dingcai wu | 2017 | Science China Chemistry2017，60，8： | 2 |
| 10 | Angular Distribution of Element Contents in Tree Rings and the Environmental Information显示文摘Element contents of tree rings and soils near tree roots collected from Deodar cedar (Cedrus deodara (Roxb.) G. Don) and Masson pine (Picks massoniana lamb.) were determined to study the relationship between the angular distribution of element contents in tree rings and the environmental information. The chemical composition and properties of soils are very much complicated, which leads to the non-uniform distribution of the element contents in tree rings. The statistical multi-variable regression method was used to got the information of the tree-centered distribution of element contents in the environment (soil) (C’), C’(z, θj ), from the distribution of element contents in tree rings (C), C(Z, θi), which depends on the plane azimuth angle (θi), i. e., C=C(Z,θi), where Z is the atomic number of the element, with a satisfactory result,though this study is only a primary one. | KE SHANZHE LIU ZHENGRONG QIAN JUNLONG CHEN RUSONGI and KE XIAOKANG (Physics Department, Nanjing University, Nanjing 210095,China)(Nanjing Institute of Geogmphy and Limnology, the Chinese Academy of Sciences, Nanjing 210008, China)(Garded and Greenbelt | 1999 | Pedosphere1999，9，1： | 2 |
| 11 | A novel and smart automatic light-seeking flowerpot for monitoring flower growth environment显示文摘Although the flowerpot is widely used for indoor flowers,it cannot meet the needs of intelligent management during the uncared-for period.The objective of this study was to design a new microcontroller-based smart flowerpot.Its overall system was composed of three parts:information collection layer,automatic control layer and data transmission layer.Firstly,in the process of collecting information,the Laiyite criterion and the normalized weighted average algorithm were adopted to improve the accuracy of information collection.Secondly,for making precise control decisions,the fuzzy control was used to achieve automatic on-demand watering.Finally,the method for comparative analysis of regional light intensity was utilized to achieve light-seeking and light-supplementing.Experimental results showed that the smart flowerpot had strong anti-jamming performance for information collection,the relative soil moisture of flowers could be stably maintained near the optimum humidity(65%),and the light was well-distributed on the flower with the error angle of light-supplementing ranged from-3°to 3°. | Xihai Zhang Dong Liu Chengguo Fan Jiali Du Fanfeng Meng Junlong Fang | 2018 | International Journal of Agricultural and Biological Engineering2018，11，2： | 2 |
| 12 | Research on the energy-saving effect of energy policies in China:1982-2006显示文摘 | Chaoqing Yuan Sifeng Liu Zhigeng Fang Junlong Wu | 2009 | Energy Policy2009，37，7： | 1 |
| 13 | The relationship among energy prices and energy consumption in China显示文摘 | Chaoqing Yuan Sifeng Liu Junlong Wu | 2010 | Energy Policy2010，38，1： | 1 |
| 14 | Pressureless sintering of large-scale fine structural alumina matrix ceramic guideway materials 显示文摘 | LIU Changxia ZHANG Jianhua SUN Junlong | 2007 | Mater Sci EngA2007，444，12： | 1 |
| 15 | Loop-mediated isothermal amplification (LAMP) method based on two species-specific primer sets for the rapid identification of Chinese Babesia bovis and B. bigemina显示文摘 | Aihong Liu Guiquan Guan Pengfei Du Huitian Gou Zhijie Liu Junlong Liu Milin Ma Jifei Yang Youquan Li Qinli Niu Qiaoyun Ren Qi Bai Hong Yin Jianxun Luo | 2012 | Parasitology International2012，，4： | 1 |
| 16 | Liquiritin potentiate neurite outgrowth induced by nerve growth factor in PC12 cells显示文摘 | CHEN Zhengai WANG Junlong LIU Ruiting | 2009 | Cytoteehnology2009，60，13： | 1 |
| 17 | A VO x /meso-TiO 2 catalyst for methanol oxidation to dimethoxymethane显示文摘 | Ensheng Zhan Yong Li Junlong Liu Xiumin Huang Wenjie Shen | 2009 | Catalysis Communications2009，，15： | 1 |
| 18 | Detecting and differentiating Theileria sergenti and Theileria sinensis in cattle and yaks by PCR based on major piroplasm surface protein (MPSP)显示文摘 | Aihong Liu Guiquan Guan Zhijie Liu Junlong Liu Neil Leblanc Youquan Li Jinliang Gao Milin Ma Qinli Niu Qiaoyun Ren Qi Bai Hong Yin Jianxun Luo | 2010 | Experimental Parasitology2010，，4： | 1 |
| 19 | Research on energy-saving effect of technological progress based on Cobb-Douglas production function显示文摘 | CHAOQING YUAN SIFENG LIU JUNLONG WU | 2009 | Energy Policy2009，37，2： | 1 |
| 20 | Our expedition in the construction of fluorescent supramolecular metallacycles显示文摘During the past few years, the construction of fluorescent supramolecular metallocycles has attracted extensive attention due to their diverse applications such as sensing, photoelectric devices, and mimicking complicated natural photo-processes. In this review, we will discuss how we entered the field of fluorescent supramolecular metallacycles and what we investigated in this field. The preparation of various fluorescent supramolecular metallacycles and their applications in monitoring the dynamics of coordination-driven self-assembly, sensing, catalysts, and supramolecular gels will be summarized. | Junlong Zhu Xi Liu Junhai Huang Lin Xu | 2019 | Chinese Chemical Letters2019，30，10： | 1 |
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