|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Acupuncture Treatment for Post-Stroke Dysphagia: An Update Meta-Analysis of Randomized Controlled Trials显示文摘Objective: To explore the effectiveness and safety of acupuncture in patients with post-stroke dysphagia by an update meta-analysis. Methods: Potentially eligible RCTs aimed to evaluate the effects of acupuncture vs. non-acupuncture treatments, such as rehabilitation training or routine medication on the swallowing difficulty after stroke were searched from Pub Med, Cochrane Library, China National Knowledge Infrastructure, and other database from the earliest record to June 2016. Patient demographics, regimens for acupuncture, type of controls, methods of randomization, and measurements of the clinical symptoms of dysphagia were retrieved. The relative risk(RR) and 95% confidence interval(CI) of effective rate of dysphagia was calculated after intervention performed following admission. Subgroup analyses and a metaregression analysis were performed to describe the heterogeneity. Results: Twenty-nine RCTs comprising 2,190 patients were included. The included studies had a medium quality grade based on the Consolidated Standards of Reporting Trials(CONSORT) and Standards for Reporting Interventions in Clinical Trials of Acupuncture(STRICTA) checklist. Acupuncture therapy provided a higher effective rate compared with nonacupuncture treatments [RR=1.33, 95% confidence interval(CI), 1.25 to 1.43]. Subgroup and meta-regression analyses suggested that acupuncture intensity and measurement method were main sources of heterogeneity and resulted in a significant difference for pooled effect size. No severe adverse events were documented in these RCTs. Conclusions: Our meta-analysis provides a new evidence supporting the efficacy and safety of acupuncture in treatment to post-stroke dysphagia in short-term compared with rehabilitation or medication. More high-quality and large-scale research studies are needed. | LI Ling-xin DENG Kai QU Yun | 2018 | Chinese Journal of Integrative Medicine2018，24，9： | 24 |
| 2 | Evaluation of the Effects of Standard Rescue Procedure on Severe Trauma Treatment in China显示文摘 | Xiao-Feng Yin Tian-Bing Wang Pei-Xun Zhang Yu-Hui Kou Dian-Ying Zhang Kai Yu De-Cheng Lyu Mao-Zheng Liu Dong-Sheng Zhou Peng Zhang Jue-Hua Jing Wei-Wei Ge Li-Ying Cao Guo-Sheng Wang Shao-Jie Deng Weng-Hua Liu Mao Zhang Yong-An Xu Kun Zhang Bing Li Wei Wang Zhong-Li Gao Cheng-La Yi Bao-Guo Jiang | 2015 | Chinese Medical Journal2015，，10： | 18 |
| 3 | Human monoclonal antibodies block the binding of SARS-CoV-2 spike protein to angiotensin converting enzyme 2 receptor显示文摘According to the World Health Organization(WHO)newly updated situation report on March 18th,2020,the coronavirus disease 2019(COVID-19)pandemic has confirmed 191,127 cases and claimed 7807 deaths worldwide.1 The etiological agent of COVID-19 has been identified as a novel coronavirus,the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),belonging to Sarbecovirus subgenus(genus Betacoronavirus,family Coronaviridae)and showing 79.6 and 96.2%sequence identity in nucleotide to SARS-CoV and a bat coronavirus(BatCoV RaTG13),respectively.2–4 Like SARS-CoV infection,a substantial fraction of COVID-19 patients exhibits severe respiratory symptoms and has to be hospitalized in intensive care unit.5–8 Although the mortality rate of COVID-19 is significantly lower than that of SARS-CoV infection,SARS-CoV-2 shows much higher human-to-human transmission rate,rapidly leading to a global pandemic declared by WHO on March 11th,2020. | Xiangyu Chen Ren Li Zhiwei Pan Chunfang Qian Yang Yang Renrong You Jing Zhao Pinghuang Liu Leiqiong Gao Zhirong Li Qizhao Huang Lifan Xu Jianfang Tang Qin Tian Wei Yao Li Hu Xiaofeng Yan Xinyuan Zhou Yuzhang Wu Kai Deng Zheng Zhang Zhaohui Qian Yaokai Chen Lilin Ye | 2020 | Cellular & Molecular Immunology2020，17，6： | 17 |
| 4 | Dynamic Crushing Strength Analysis of Auxetic Honeycombs显示文摘The in-plane dynamic crushing behavior of re-entrant honeycomb is analyzed and compared with the conventional hexagon topology.Detailed deformation modes along two orthogonal directions are examined,where a parametric study of the effect of impact velocity and cell wall aspect ratio is performed.An analytical formula of the dynamic crushing strength is then deduced based on the periodic collapse mechanism of cell structures.Comparisons with the finite element results validate the effectiveness of the proposed analytical method.Numerical results also reveal higher plateau stress of re-entrant honeycomb over conventional hexagon topology,implying better energy absorption properties.The underlying physical understanding of the results is emphasized,where the auxetic effect(negative Poisson's ratio) induced in the re-entrant topology is believed to be responsible for this superior impact resistance. | Xiuhui Hou Zichen Deng Kai Zhang | 2016 | Acta Mechanica Solida Sinica2016，29，5： | 16 |
| 5 | Oroxindin inhibits macrophage NLRP3 inflammasome activation in DSS-induced ulcerative colitis in mice via suppressing TXNIP-dependent NF-κB pathway显示文摘Oroxindin is a flavonoid isolated from the traditional Chinese medicine Huang-Qin,which has shown various pharmacological activities including anti-inflammatory,antitumor,antioxidant,etc.Thus far,the effect of oroxindin on colonic inflammation and the underlying mechanism remain unknown.In this study,we investigated the tissue distribution of oroxindin and its therapeutic effects on ulcerative colitis(UC)as well as the underlying mechanisms.UC model was established in mice by administrating dextran sulfate sodium(DSS)in drinking water for 7 d.We first showed that oroxindin was largely absorbed by the colon as an active ingredient after normal mice received Huang-Qin-Tang,a traditional Chinese medicine decoction.UC mice were then treated with oroxindin(12.5,25,50 mg-kg^-1·d^-1,i.g.)for 10 d.We found that oroxindin treatment greatly suppressed massive macrophages infiltration and attenuated pathological changes in colonic tissue.Furthermore,oroxindin treatment significantly inhibited the generation of IL-13 and IL-18 in the colon via inhibiting the nucleotide-binding oligomerization domain-like receptor 3(NLRP3)inflammasome formation and activation.In cultured macrophages,LPS induced NLRp3 inflammasome formation and caspase-1 activation,which were suppressed by oroxindin(12.5-50μM).In LPS-treated macrophages,oroxindin dose-dependently restored the expression of TXNIP protein,leading to suppressing TXNIP-dependent NF-κB activation.In conclusion,these results demonstrate that oroxindin could be absorbed by the colon and attenuate inflammatory responses via inhibiting NLRP3 inflammasome formation and activation,which is related to the inhibitory effect on TXNIP-dependent NF-κB-signaling pathway.Hence,oroxindin has the potential of becoming an effective drug for treating UC. | Qi Liu Rui Zuo Kai Wang Fei-fei Nong Ya-jun Fu Shao-wei Huang Zeng-feng Pan Yi Zhang Xia Luo Xiang-liang Deng Xiao-xue Zhang Lian Zhou Yang Chen | 2020 | Acta Pharmacologica Sinica2020，41，6： | 15 |
| 6 | PARylation regulates stress granule dynamics, phase separation, and neurotoxicity of disease-related RNA-binding proteins显示文摘Mutations in RNA-binding proteins (RBPs) localized in ribonucleoprotein (RNP) granules, such as hnRNP A1 and TDP-43, promote aberrant protein aggregation, which is a pathological hallmark of various neurodegenerative diseases, such as amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). Protein posttranslational modifications (PTMs) are known to 佗gulate RNP granules. In this study, we investigate the function of poly(ADP-ribosyl)ation (PARylation), an important PTM involved in DNA damage repair and cell death, in RNP granule-related neurodegeneration. We reveal that PARylation levels are a major regulator of the assembly-disassembly dynamics of RNP granules containing disease-related RBPs, hnRNP A1 and TDP-43. We find that hnRNP A1 can both be PARylated and bind to PARylated proteins or poly(ADP-ribose)(PAR). We further uncover that PARylation of hnRNP A1 at K298 controls its nucleocytoplasmic transport, whereas PAR-binding via the PAR-binding motif (PBM) of hnRNP Al regulates its association with stress granules. Moreover, we reveal that PAR not only dramatically enhances the liquid-liquid phase separation of hnRNP A1, but also promotes the co-phase separation of hnRNP A1 and TDP-43 in vitro and their interaction in vivo. Finally, both genetic and pharmacological inhibition of PARP mitigates hnRNP Al- and TDP-43-mediated neurotoxicity in cell and Drosophila models of ALS. Together, our findings suggest a novel and crucial role for PARylation in regulating the dynamics of RNP granules, and that dysregulation in PARylation and PAR levels may con tribute to ALS disease pathoge nesis by promoting protei n aggregation. | Yongjia Duan Aiying Du Jinge Gu Gang Duan Chen Wang Xinrui Gui Zhiwei Ma Beituo Qian Xue Deng Kai Zhang Le Sun Kuili Tian Yaoyang Zhang Hong Jiang Cong Liu Yanshan Fang | 2019 | Cell Research2019，29，3： | 13 |
| 7 | Piglets cloned from induced pluripotent stem cells显示文摘 | Nana Fan Jijun Chen Zhouchun Shang Hongwei Dou Guangzhen Ji Qingjian Zou Lu Wu Lixiazi He Fang Wang Kai Liu Na Liu Jianyong Han Qi Zhou Dengke Pan Dongshan Yang Bentian Zhao Zhen Ouyang Zhaoming Liu Yu Zhao Lin Lin Chongming Zhong Quanlei Wang Shouqi Wang Ying Xu Jing Luan Yu Liang Zhenzhen Yang Jing Li Chunxia Lu Gabor Vajta Ziyi Li Hongsheng Ouyang Huayan Wang Yong Wang Yang Yang Zhonghua Liu Hong Wei Zhidong Luan Miguel A Esteban Hongkui Deng Huanming Yang Duanqing Pei Ning Li Gang Pei Lin Liu Yutao Du Lei Xiao Liangxue Lai | 2013 | Cell Research2013，23，1： | 12 |
| 8 | miRNA-338-3p suppresses cell growth of human colorectal carcinoma by targeting smoothened显示文摘AIM:To investigate the regulative effect of miRNA-3383p(miR-338-3p) on cell growth in colorectal carcinoma(CRC).METHODS:The lentiviral vector pLV-THM-miR-338-3p and pLV-THM-miR-338-3p-inhibitor were constructed.The recombinant viral vector encoding the pre-miR338-3p or miR-338-3p-inhibitor and the two packaging plasmids psPAX2 and pMD2.G were cotransfected into human embryonic kidney 293T cells to package lentivirus.The supernatant containing the lentivirus particles was harvested to determine the viral titer,and this supernatant was then used to transduce CRCderived cell line,SW-620.Flow cytometry was utilized for sorting the green fluorescent protein(GFP) + cells to establish the SW-620 cell line stably expressing premiR-338-3p or miR-338-3p-inhibitor.Moreover,the expression of miR-338-3p was determined by real-time reverse transcriptase polymerase chain reaction,andWestern blotting was used to detect the expression of the smoothened(SMO,the possible target of miR-3383p) protein in SW-620 cells.Furthermore,the status of CRC cell proliferation and apoptosis were detected by 3-(4,5-dimethyl-2 thiazoyl)-2,5-diphenyl-2H-tetrazolium bromide assay and flow cytometry,respectively.RESULTS:Restriction enzyme digestion and DNA sequencing demonstrated that the lentiviral vector pLVTHM-miR-338-3p and pLV-THM-miR-338-3p-inhibitor were constructed successfully.GFP was expressed after the SW-620 cells were transduced by the lentivirus.Expression of miR-338-3p in SW-620 cells transduced with the lentivirus pLV-THM-miR-338-3p was significantly increased(relative expression 3.91 ± 0.51 vs 2.36 ± 0.44,P < 0.01).Furthermore,overexpression of miR-338-3p inhibited the expression of SMO protein in SW-620 cells,which showed obviously suppressed proliferation ability [cellular proliferation inhibition rate(CPIR) 61.9% ± 5.2% vs 41.6% ± 4.8%,P < 0.01].Expression of miR-338-3p in SW-620 cells transduced with the lentivirus pLV-THM-miR-338-3p-inhibitor was significantly decreased(relative expression 0.92 ± 0.29 vs 2.36 ± 0.44,P < 0.01).Moreover,the downregulated expression of miR-338-3p caused upregulated expression of the SMO protein in SW-620 cells,which showed significantly enhanced proliferation ability(CPIR 19.2% ± 3.8% vs 41.6% ± 4.8%,P < 0.01).However,anti-SMO-siRNA largely,but not completely,reversed the effects induced by blockage of miR-338-3p,suggesting that the regulative effect of miR-338-3p on CRC cell growth was indeed mediated by SMO.CONCLUSION:miR-338-3p could suppress CRC growth by inhibiting SMO protein expression. | Kai Sun Hai-Jun Deng Shang-Tong Lei Jing-Qing Dong Guo-Xin Li | 2013 | World Journal of Gastroenterology2013，19，14： | 12 |
| 9 | Circular RNA circVAPA promotes chemotherapy drug resistance in gastric cancer progression by regulating miR-125b-5p/STAT3 axis显示文摘BACKGROUND Gastric cancer(GC)is a prevalent malignancy,leading to a high incidence of cancer-associated death.Cisplatin(DDP)-based chemotherapy is the principal therapy for clinical GC treatment,but DDP resistance is a severe clinical challenge and the mechanism remains poorly understood.Circular RNAs(circRNAs)have been identified to play crucial roles in modulating the chemoresistance of gastric cancer cells.AIM To explore the effect of circVAPA on chemotherapy resistance during GC progression.METHODS The effect of circVAPA on GC progression and chemotherapy resistance was analyzed by MTT assay,colony formation assay,Transwell assay,wound healing assay,and flow cytometry analysis in GC cells and DDP resistant GC cell lines,and tumorigenicity analysis in nude mice in vivo.The mechanism was investigated by luciferase reporter assay,quantitative real-time PCR,and Western blot analysis.RESULTS CircVAPA expression was up-regulated in clinical GC tissues compared with normal samples.CircVAPA depletion inhibited proliferation,migration,and invasion and increased apoptosis of GC cells.The expression of circVAPA,STAT3,and STAT3 downstream genes was elevated in DDP resistant SGC7901/DDP cell lines.CircVAPA knockdown attenuated the DDP resistance of GC cells.Mechanically,circVAPA was able to sponge miR-125b-5p,and miR-125b-5p could target STAT3 in the GC cells.MiR-125b-5p inhibitor reversed circVAPA depletion-enhanced inhibitory effect of DDP on GC cells,and STAT3 knockdown blocked circVAPA overexpression-induced proliferation of DDPtreated SGC7901/DDP cells.The depletion of STAT3 and miR-125b-5p inhibitor reversed circVAPA depletion-induced GC cell apoptosis.Functionally,circVAPA contributed to the tumor growth of SGC7901/DDP cells in vivo.CONCLUSION CircVAPA promotes chemotherapy resistance and malignant progression in GC by miR-125b-5p/STAT3 signaling.Our findings present novel insights into the mechanism by which circVAPA regulates chemotherapy resistance of GC cells.CircVAPA and miR-125b-5p may be considered as the potential targets for GC therapy. | Peng Deng Ming Sun Wen-Yan Zhao Bin Hou Kai Li Tao Zhang Feng Gu | 2021 | World Journal of Gastroenterology2021，27，6： | 11 |
| 10 | Retrospective evaluation of lymphatic and blood vessel invasion and Borrmann types in advanced proximal gastric cancer显示文摘BACKGROUND The Borrmann classification system is used to describe the macroscopic appearance of advanced gastric cancer,and Borrmann typeⅣdisease is independently associated with a poor prognosis.AIM To evaluate the prognostic significance of lymphatic and/or blood vessel invasion(LBVI)combined with the Borrmann type in advanced proximal gastric cancer(APGC).METHODS The clinicopathological and survival data of 440 patients with APGC who underwent curative surgery between 2005 and 2012 were retrospectively analyzed.RESULTS In these 440 patients,LBVI+status was associated with Borrmann typeⅣ,low histological grade,large tumor size,and advanced pT and pN status.The 5-year survival rate of LBVI+patients was significantly lower than that of LBVI– patients,although LBVI was not an independent prognostic factor in the multivariate analysis.No significant difference in the prognosis of patients with Borrmann typeⅢ/LBVI+disease and patients with Borrmann typeⅣdisease was observed.Therefore,we proposed a revised Borrmann typeⅣ(r-BorⅣ)as Borrmann typeⅢplus LBVI+,and found that r-BorⅣwas associated with poor prognosis in patients with APGC,which outweighed the prognostic significance of pT status.CONCLUSION LBVI is related to the prognosis of APGC,but is not an independent prognostic factor.LBVI status can be used to differentiate Borrmann typesⅢandⅣ,and the same approach can be used to treat r-BorⅣand Borrmann typeⅣ. | Shan Gao Guo-Hui Cao Peng Ding Yang-Yang Zhao Peng Deng Bin Hou Kai Li Xiao-Fang Liu | 2019 | World Journal of Gastrointestinal Oncology2019，11，8： | 11 |
| 11 | Cerebral artery evaluation of dual energy CT angiography with dual source CT显示文摘背景常规计算断层摄影术 angiography (CTA ) 是耗时的,用户依赖者并且在颅骨库区域有差的图象质量。这研究估计了一个新方法的可行性,为描绘服的 artery.Methods 幽灵扫描的双精力 CTA 为放射剂量计算在双来源 CT 和 64 螺线 CT 上用完了头 CTA 序列。双精力 CTA 在被怀疑有服的脉管的疾病的 36 个病人上用完了双来源 CT。三个系列在 0.75 公里的轴的图象厚, 0.4 公里增长被获得,它与 80 kV, 140 kV 和合并图象被说出;80 kV 和 140 幅 kV 图象被变成双精力软件,和最大的紧张设计(MIP ) 图象被双精力骨头快速产生搬迁(DEBR 组) ;合并图象被变成在空间软件搬迁通过用手的常规骨头获得 MIP 图象(CoBR 组) 。波斯特处理时间和读物时间被比较。二个组的图象质量被比较,主要集中于内部颈动脉动脉和骨头减法的颅骨底片断。ANOVA 和 SNK 测试被申请放射剂量比较。学生的 t 测试和 Wilcoxon 等级和测试被申请为意义估计数据之间的差别。科恩的 kappa 被用于 interobserver 同意。幽灵扫描的结果放射剂量证明双精力 CTA 在数字骨头减法和常规 CTA 之间。处理时间和读物时间的帖子在颅骨底比 CoBR,和图象质量在 DEBR 是短得多的比 CoBR 在 DEBR 是高得多的(P < 0.01 ) 。为在二个组之间的 suprasellar 容器没有重要差别(P > 0.5 ) 。为所有容器片断的 Interobserver 同意是优秀的(kappa=0.97 ) 。结论双精力 CTA 是为描绘服的动脉的一种可靠、新形式,克服在颅骨底区域的常规 CTA 的限制。它能在柱子比常规 CTA 处理并且读节省许多时间。 | MA Rui LIU Cheng DENG Kai SONG Shao-juan WANG Dao-ping HUANG Ling | 2010 | Chinese Medical Journal2010，，9： | 11 |
| 12 | Perivascular adipose tissue dysfunction aggravates adventitial remodeling in obese mini pigs via NLRP3 inflammasome/IL-1 signaling pathway显示文摘Perivascular adipose tissue (PVAT), a special type of adipose tissue, closely surrounds vascular adventitia and produces numerous bioactive substances to maintain vascular homeostasis. PVAT dysfunction has a crucial role in regulating vascular remodeling, but the exact mechanisms remain unclear. In this study, we investigated whether and how obesity-induced PVAT dysfunction affected adventitia remodeling in early vascular injury stages. Mini pigs were fed a high sugar and fat diet for 6 months to induce metabolic syndrome and obesity. In the mini pigs, left carotid vascular injury was then generated using balloon dilation. Compared with normal mini pigs, obese mini pigs displayed significantly enhanced vascular injury-induced adventitial responses, evidenced by adventitia fibroblast (AF) proliferation and differentiation, and adventitia fibrosis, as well as exacerbated PVAT dysfunction characterized by increased accumulation of resident macrophages, particularly the M1 pro-infiammatory phenotype, increased expression of leptin and decreased expression of adiponectin, and production of pro-infiammatory cytokines interleukin (IL)-1β and IL-18. Primary AFs cultured in PVAT-conditioned medium from obese mini pigs also showed significantly increased proliferation and differentiation. We further revealed that activated nod-like receptor protein 3 (NLRP3) infiammasome and its downstream products, i.e., IL-1 family members such as IL-1β and IL-18 were upregulated in the PVAT of obese mini pigs;PVAT dysfunction was also demonstrated in preadipocytes treated with palmitic acid. Finally, we showed that pretreatment with IL-1 receptor (IL-1R) antagonist or IL-1R knockdown blocked AF proliferation and differentiation in AFs cultured in PVAT-conditioned medium. These results demonstrate that obesity-induced PVAT dysfunction aggravates adventitial remodeling after early vascular injury with elevated AF proliferation and differentiation via activating the NLRP3/IL-1 signaling pathway. | Xiao Zhu Hong-wen Zhang Hai-nan Chen Xiao-jun Deng Yi-xuan Tu Ampadu O. Jackson Ji-na Qing Ai-ping Wang Vaibhav Patel Kai Yin | 2019 | Acta Pharmacologica Sinica2019，40，1： | 9 |
| 13 | Prioritizing natural-selection signals from the deep-sequencing genomic data suggests multi-variant adaptation in Tibetan highlanders显示文摘Human genetic adaptation to high altitudes(>2500 m)has been extensively studied over the last few years,but few functional adaptive genetic variants have been identified,largely owing to the lack of deep-genome sequencing data available to previous studies.Here,w e build a list of putative adaptive variants,including 63 missense,7 loss-of-function;1,298 evolutionarily conserved variants and 509 expression quantitative traits loci.Notably,the top signal of selection is located in TMEM 247,a transmembrane protein-coding gene.The Tibetan version of TMEM 247 harbors one high-frequency(76.3%)missense variant,rsl 16983452(c.248C>T;p.Ala83Val);with the T allele derived from archaic ancestry and carried by>94%of Tibetans but absent or in low frequencies(<3%)in non-Tibetan populations.The rsl 16983452-T is strongly and positively correlated with altitude and significantly associated with reduced hemoglobin concentration(p=5.78×10^-5),red blood cell count(p=5.72×10^-7)and hematocrit(p=2.57×10^-6).In particular,TMEM247-rs 116983452 shows greater effect size and better predicts the phenotypic outcome than any E P A S1 variants in association with adaptive traits in Tibetans.Modeling the interaction between TM£M247-rsl 16983452 and EPAS1 variants indicates weak but statistically significant epistatic effects.Our results support that multiple variants may jointly deliver the fitness of the Tibetans on the plateau,where a complex model is needed to elucidate the adaptive evolution mechanism. | Lian Deng Chao Zhang Kai Yuan Yang Gao Yuwen Pan Xueling Ge Yaoxi He Yuan Yuan Yan Lu Xiaoxi Zhang Hao Chen Haiyi Lou Xiaoji Wang Dongsheng Lu Jiaojiao Liu Lei Tian Qidi Feng Asifullah Khan Yajun Yang Zi-Bing Jin Jian Yang Fan Lu Jia Qu Longli Kang Bing Su Shuhua Xu | 2019 | National Science Review2019，6，6： | 9 |
| 14 | Psychological impact of the COVID-19 pandemic on healthcare workers:a cross-sectional study in China显示文摘Background Healthcare workers fighting against the coronavirus disease 2019(COVID-19)pandemic are under tremendous pressure,which puts them at an increased risk of developing psychological problems.Aims This study aimed to investigate the prevalence of psychological problems in different healthcare workers(ie,physicians,medical residents,nurses,technicians and public health professionals)during the COVID-19 pandemic in China and explore factors that are associated with the onset of psychological problems in this population during this public health crisis.Methods A cross-sectional,web-based survey was conducted in February 2020 among healthcare workers during the COVID-19 pandemic.Psychological problems were assessed using the Generalized Anxiety Disorder Scale,Patient Health Questionnaire and Insomnia Severity Index.Logistic regression analyses were used to explore the factors that were associated with psychological problems.Results The prevalence of symptoms of anxiety,depression,insomnia and the overall psychological problems in healthcare workers during the COVID-19 pandemic in China was 46.04%,44.37%,28.75%and 56.59%,respectively.The prevalence of the overall psychological problems in physicians,medical residents,nurses,technicians and public health professionals was 60.35%,50.82%,62.02%,57.54%and 62.40%,respectively.Compared with healthcare workers who did not participate in front-line work,front-line healthcare workers had a higher risk of anxiety,insomnia and overall psychological problems.In addition,attention to negative or neutral information about the pandemic,receiving negative feedback from families and friends who joined front-line work,and unwillingness to join front-line work if given a free choice were three major factors for these psychological problems.Conclusions Psychological problems are pervasive among healthcare workers during the COVID-19 pandemic.Receiving negative information and participating in front-line work appear to be.important risk factors for psychological problems.The psychological health of different healthcare workers should be protected during the COVID-19 pandemic with timely interventions and proper information feedback. | Jianyu Que Le Shi Jiahui Deng Jiajia Liu Li Zhang Suying Wu Yimiao Gong Weizhen Huang Kai Yuan Wei Yan Yankun Sun Maosheng Ran Yanping Bao Lin Lu | 2020 | General Psychiatry2020，33，3： | 8 |
| 15 | Human iPSCs derived astrocytes rescue rotenone-induced mitochondrial dysfunction and dopaminergic neurodegeneration in vitro by donating functional mitochondria显示文摘Background Parkinson’s disease(PD)is one of the neurodegeneration diseases characterized by the gradual loss of dopaminergic(DA)neurons in the substantia nigra region of the brain.Substantial evidence indicates that at the cellular level mitochondrial dysfunction is a key factor leading to pathological features such as neuronal death and accumulation of misfoldedα-synuclein aggregations.Autologous transplantation of healthy purified mitochondria has shown to attenuate phenotypes in vitro and in vivo models of PD.However,there are significant technical difficulties in obtaining large amounts of purified mitochondria with normal function.In addition,the half-life of mitochondria varies between days to a few weeks.Thus,identifying a continuous source of healthy mitochondria via intercellular mitochondrial transfer is an attractive option for therapeutic purposes.In this study,we asked whether iPSCs derived astrocytes can serve as a donor to provide functional mitochondria and rescue injured DA neurons after rotenone exposure in an in vitro model of PD.Methods We generated DA neurons and astrocytes from human iPSCs and hESCs.We established an astroglial-neuronal co-culture system to investigate the intercellular mitochondrial transfer,as well as the neuroprotective effect of mitochondrial transfer.We employed immunocytochemistry and FACS analysis to track mitochondria.Results We showed evidence that iPSCs-derived astrocytes or astrocytic conditioned media(ACM)can rescue DA neurons degeneration via intercellular mitochondrial transfer in a rotenone induced in vitro PD model.Specifically,we showed that iPSCs-derived astrocytes from health spontaneously release functional mitochondria into the media.Mito-Tracker Green tagged astrocytic mitochondria were detected in the ACM and were shown to be internalized by the injured neurons via a phospho-p38 depended pathway.Transferred mitochondria were able to significantly reverse DA neurodegeneration and axonal pruning following exposure to rotenone.When rotenone injured neurons were cultured in presence of ACM depleted of mitochondria(by ultrafiltration),the neuroprotective effects were abolished.Conclusions Our studies provide the proof of principle that iPSCs-derived astrocytes can act as mitochondria donor to the injured DA neurons and attenuate pathology.Using iPSCs derived astrocytes as a donor can provide a novel strategy that can be further developed for cellular therapy for PD. | Xiao-Yu Cheng Sangita Biswas Juan Li Cheng-Jie Mao Olga Chechneva Jing Chen Kai Li Jiao Li Jin-Ru Zhang Chun-Feng Liu Wen-Bin Deng | 2020 | Translational Neurodegeneration2020，9，2： | 7 |
| 16 | FBW7 regulates endothelial functions by targeting KLF2 for ubiquitination and degradation显示文摘F 盒子和 WD 重复包含域 7 (FBW7 ) , E3 ubiquitin ligase SCF FBW7 (SKP1, cullin-1 和 FBW7 的建筑群) ,在各种各样的生理、病理学的过程起重要作用。尽管 FBW7 为脉管的开发被要求,它在内皮细胞层的函数尚待被调查。在这研究,我们证明 FBW7 是 endothelial 功能的一个重要管理者,包括 angiogenesis,白血球粘附和 endothelial 障碍正直。用 RNA 干扰,我们发现 FBW7 的弄空显著地在 vitro 并且在 vivo 损害 angiogenesis。我们识别了锌手指抄写因素 Krü象 ppel 一样因素 2 (KLF2 ) 作为在 endothelial 房间的 FBW7 的一个生理的目标。FBW7 击倒表示在 endothelial 房间导致了内长的 KLF2 蛋白质的累积。调停 FBW7 的 KLF2 破坏被显示在二保存 phosphodegrons 经由肝糖 synthase kinase-3 (GSK3 ) 取决于 KLF2 的 phosphorylation。变异这些 phosphodegron 主题废除了 KLF2 的调停 FBW7 的降级和 ubiquitination。调停 siRNA 显示出的 FBW7 击倒 KLF2 是在 endothelial 的规定的 FBW7 的一个必要目标,这工作。而且,调停 FBW7 的 KLF2 降级被显示为在 teratomas 并且在 zebrafish 开发的 angiogenesis 批评。一起拿,我们的学习在 endothelial 房间移植, angiogenesis,发炎和障碍完整的进程为 FBW7 建议一个角色,并且在 vivo 提供新奇卓见进 KLF2 稳定性的规定。 | Rui Wang Yah Wang Ning Liu Chunguang Ren Cong Jiang Kai Zhang Su Yu Yunfei Chen Hui Tang Qi Deng Cong Fu Yingcong Wang Rong Li Mingyao Liu Weijun Pan Ping Wang | 2013 | Cell Research2013，23，6： | 7 |
| 17 | Genome Characterization of the First Outbreak of COVID-19 Delta Variant B.1.617.2——Guangzhou City,Guangdong Province,China,May 2021显示文摘On May 20,2021,75-year-old female(Case A)went to a local hospital due to pharyngeal pain and low-grade fever in Liwan District,Guangzhou City,Guangdong Province.The oropharyngeal swab from Case A tested preliminarily positive for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)/coronavirus disease 2019(COVID-19)virus using a quantitative real-time reverse transcription polymerase chain reaction(RT-qPCR)method after a hospital visit. | Zhencui Li Kai Nie Kuibiao Li Yao Hu Yang Song Min Kang Meng Zhang Xiaoling Deng Jun Yuan Wenbo Xu Baisheng Li | 2021 | China CDC weekly2021，3，27： | 7 |
| 18 | Hierarchical CuCo_2O_4@nickel-cobalt hydroxides core/shell nanoarchitectures for high-performance hybrid supercapacitors显示文摘Ni_(0.5)Co_(0.5)(OH)_2 nanosheets coated CuCo_2O_4 nanoneedles arrays were successfully designed and synthesized on carbon fabric. The core/shell nanoarchitectures directly served as the binder-free electrode with a superior capacity of 295.6 mAh g^(-1) at 1 Ag^(-1), which still maintained 220 mAh g^(-1) even at the high current density of 40 Ag^(-1), manifesting their enormous potential in hybrid supercapacitor devices. The asassembled CuCo_2O_4@Ni_(0.5)Co_(0.5)(OH)_2//AC hybrid supercapacitor device exhibited favorable properties with the specific capacitance as high as 90 Fg^(-1) at 1 Ag^(-1) and the high energy density of 32 Wh kg^(-1) at the power density of 800 Wkg^(-1). Furthermore, the as-assembled device also delivered excellent cycling performance(retaining 91.9% of the initial capacitance after 12,000 cycles at 8 Ag^(-1)) and robust mechanical stability and flexibility, implying the huge potential of present hierarchical electrodes in energy storage devices. | Chenggang Wang Kai Guo Weidong He Xiaolong Deng Peiyu Hou Fuwei Zhuge Xijin Xu Tianyou Zhai | 2017 | Science Bulletin2017，62，16： | 6 |
| 19 | Self-powered versatile shoes based on hybrid nanogenerators显示文摘摩擦电 nanogenerator (TENG ) 和一个电磁的发电机(EMG ) 是 hybridized 收获人的机械精力。由 triboelectrification 和电磁的正式就职的一个有效连词,有 1.2 厘米的 2 厘米和高度的半径的 hybridized nanogenerator 能在颤动的 100 个周期以后控告 1,000 F 电容器到 5.09 V。这微型大小的混合 nanogenerator 然后能在鞋被嵌入用作一个精力房间。典型室外的 applicationsincluding 与一台全球放系统(GPS ) 设备开车,为一节 Li 离子电池和成功地表明的房间 phonewere 充电,建议它在聪明的便携电子学和士兵的未来西装的潜在的应用程序。 | Long Liu Wei Tang Chaoran Deng Baodong Chen Kai Han Wei Zhong Zhong Lin Wang | 2018 | Nano Research2018，11，8： | 6 |
| 20 | Structural and biochemical mechanism for increased infectivity and immune evasion of Omicron BA.2 variant compared to BA.1 and their possible mouse origins显示文摘The Omicron BA.2 variant has become a dominant infective strain worldwide.Receptor binding studies show that the Omicron BA.2 spike trimer exhibits 11-fold and 2-fold higher potency in binding to human ACE2 than the spike trimer from the wildtype(WT)and Omicron BA.1 strains.The structure of the BA.2 spike trimer complexed with human ACE2 reveals that all three receptor-binding domains(RBDs)in the spike trimer are in open conformation,ready for ACE2 binding,thus providing a basis for the increased infectivity of the BA.2 strain.JMB2002,a therapeutic antibody that was shown to efficiently inhibit Omicron BA.1,also shows potent neutralization activities against Omicron BA.2.In addition,both BA.1 and BA.2 spike trimers are able to bind to mouse ACE2 with high potency.In contrast,the WT spike trimer binds well to cat ACE2 but not to mouse ACE2.The structures of both BA.1 and BA.2 spike trimer bound to mouse ACE2 reveal the basis for their high affinity interactions.Together,these results suggest a possible evolution pathway for Omicron BA.1 and BA.2 variants via a human-cat-mouse-human circle,which could have important implications in establishing an effective strategy for combating SARS-CoV-2 viral infections. | Youwei Xu Canrong Wu Xiaodan Cao Chunyin Gu Heng Liu Meriting Jiang Xiaoxi Wang Qingning Yuan Kai Wu Jia Liu Deyi Wang Xianqing He Xueping Wang Su-Jun Deng H.Eric Xu Wanchao Yin | 2022 | Cell Research2022，32，7： | 6 |
您所在的机构暂未开通此部分的功能，请联系管理员