|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Generation of human haploid embryonic stem cells from parthenogenetic embryos obtained by microsurgical removal of male pronucleus显示文摘 | Cuiqing Zhong Meiling Zhang Qi Yin Han Zhao Yang Wang Sexin Huang Wenrong Tao Keliang Wu Zi-Jiang Chen Jinsong Li | 2016 | Cell Research2016，26，6： | 16 |
| 2 | Modern pollen and vegetation relationships in the Yili Basin,Xinjiang, NW China显示文摘Fifty-six surface pollen samples from different vegetation zones in the Yili Basin,western Tianshan Mountains,Xinjiang were analyzed to examine the relationships between the surface pollen assemblages and the original vegetation.A pollen analysis and a vegetation investigation with a discriminant analysis show that the pollen assemblages greatly differ across disparate vegetation zones.Twelve pollen taxa can be used as significant types for vegetation reconstruction in the basin.These taxa were the most abundant in the surface pollen samples.Cupressaceae pollen percentages were greater than 1%in Cupressaceae shrubs.More than5%of Picea pollen indicates the growth of a Picea forest within 5 km.The subalpine meadow that is distributed widely in the basin is characterized by high content of Artemisia,Chenopodiaceae,Poaceae,Picea,Asteraceae,Taraxacum and Arenaria pollen types.The Artemisia-Chenopodiaceae-Poaceae-Cannabaceae pollen assemblages indicate the presence of montane steppe in the area.Artemisia and Chenopodiaceae pollen dominate the desert steppe and Populus forest.Artemisia pollen percentages were greater than 60%in the Artemisia desert,whereas Chenopodiaceae pollen percentages exceeded 65%in the Chenopodiaceae desert.The Artemisia/Chenopodiaceae(A/C)ratios reflect the vertical moisture changes in the Yili Basin.The mean A/C ratios were greater than 1.2 in the subalpine meadow and montane steppe that occupy the humid zone in the basin.These ratios were between1 and 1.2 in the Cupressaceae shrubs,desert steppe,Populus forest and floodplain meadow.The ratios were less than 0.5 in the Chenopodiaceae desert,which is an arid environment. | ZHAO KeLiang LI XiaoQiang | 2013 | Chinese Science Bulletin2013，58，33： | 14 |
| 3 | Polar bodies are efficient donors for reconstruction of human embryos for potential mitochondrial replacement therapy显示文摘 | Keliang Wu Cuiqing Zhong Tailai Chen Xiaoyu Zhang Wenrong Tao Jingye Zhang Hongchang Li Han Zhao Jinsong Li Zi-Jiang Chen | 2017 | Cell Research2017，27，8： | 13 |
| 4 | Reproductive management through integration of PGD and MPS-based noninvasive prenatal screening/diagnosis for a family with GJB2-associated hearing impairment显示文摘A couple with a proband child of GJB2(encoding the gap junction protein connexin 26)-associated hearing impairment and a previous pregnancy miscarriage sought for a reproductive solution to bear a healthy child. Our study aimed to develop a customized preconception-to-neonate care trajectory to fulfill this clinical demand by integrating preimplantation genetic diagnosis(PGD), noninvasive prenatal testing(NIPT), and noninvasive prenatal diagnosis(NIPD) into the strategy. Auditory and genetic diagnosis of the proband child was carried out to identify the disease causative mutations. The couple then received in-vitro-fertilization treatment, and eight embryos were obtained for day 5 biopsy. PGD was performed by short-tandem-repeat linkage analysis and Sanger sequencing of GJB2 gene. Transfer of a GJB2 c.235del C heterozygous embryo resulted in a singleton pregnancy. At the 13 th week of gestation, genomic DNA(g DNA) from the trio family and cell-free DNA(cf DNA) from maternal plasma were obtained for assessment of fetal chromosomal aneuploidy and GJB2 mutations. NIPT and NIPD showed the absence of chromosomal aneuploidy and GJB2-associated disease in the fetus, which was later confirmed by invasive procedures and postnatal genetic/auditory diagnosis. This strategy successfully prevented the transmission of hearing impairment in the newborn, thus providing a valuable experience in reproductive management of similar cases and potentially other monogenic disorders. | XIONG WenPing WANG DaYong GAO Yuan GAO Ya WANG HongYang GUAN Jing LAN Lan YAN JunHao ZONG Liang YUAN Yuan DONG Wei HUANG SeXin WU KeLiang WANG YaoShen WANG ZhiLi PENG HongMei LU YanPing XIE LinYi ZHAO Cui WANG Li ZHANG QiuJing GAO Yun LI Na YANG Ju YIN ZiFang HAN Bing WANG Wei CHEN Zi-Jiang WANG QiuJu | 2015 | Science China(Life Sciences)2015，58，9： | 12 |
| 5 | Early agricultural development and environmental effects in the Neolithic Longdong basin(eastern Gansu)显示文摘Neolithic agricultural development and environmental effects in the Longdong area were reconstructed using a synthetic approach,investigating pollen,charcoal,and seed remains for two cultural layer sections and five flotation sites.Results show that Neolithic agriculture in the Longdong area had a simple organization and was dominated by the production of common millet,especially in the early and middle Yangshao age.After the late Yangshao age,Neolithic agriculture developed into a more complex structure,dominated by both common and foxtail millet and the cultivation of rice and soybeans.The production of foxtail millet gradually increased through the Neolithic period,reaching its highest point during the Qijia culture.Soybeans were first cultivated during the late Yangshao culture,approximately 5000 cal a BP.Rice production began no later than 4800 cal a BP,and continued to exist in the Qijia culture,approximately 4000 cal a BP.Agricultural production in Neolithic Longdong,specifically in the 'Yuan' area of the loess plateau,developed as a shrub and grass dominated landscape.Vegetation in the river valleys was partly covered with Picea,Tusga,and Quercus coniferous and broadleaf mixed forests.Agricultural activity during the Neolithic period caused an increase in farmland on the loess tableland and a decrease in the abundance of shrub and grassland in the Longdong area.When farmlands were abandoned,vegetation recovered with Hippophae-,Rosaceae-,Ephedra-,and Leguminosae-dominated shrublands and Artemisia-dominated grasslands. | ZHOU XinYing LI XiaoQiang ZHAO KeLiang DODSON John SUN Nan YANG Qing | 2011 | Chinese Science Bulletin2011，56，8： | 10 |
| 6 | Investigation of the ultrastructural characteristics of foxtail and broomcorn millet during carbonization and its application in archaeobotany显示文摘Fossilized caryopses (or grains) of foxtail millet (Setaria italica) and broomcorn millet (Panicum miliaceum) are important archaeobotanical materials for the study of early human agricultural activities.The morphology and ultrastructural characteristics of carbonized modern millets caryopses treated in a drying oven and burning in a field were investigated at different temperatures to study how fossilized millets are formed.The caryopses shrank gradually at temperatures below 200°C,and starch granules in the endosperm retained their crystalline structure.At 250°C the foxtail millet caryopses expanded,whereas the broomcorn millet caryopses were greatly deformed.At this temperature,the structure of the starch granules of both millets became amorphous.At 300°C the caryopses partially turned to ash and became porous,and the ultrastructure of the starch granules was transformed into alveolate cavities.Fossil caryopses from the prehistoric storage cellar at the Beiniu Site retained their crystalline structure and were formed by the dehydrating effect of carbonization,indicating that water molecules were not involved in the starch crystallization.The results of a field burning experiment demonstrated that the ultrastructure of carbonized caryopses placed on the ground under the fire was amorphous.The amorphous ultrastructure of the carbonized caryopses recovered from the archaeological layers is consistent with the expected structure of caryopses that have been carbonized at 250°C.Therefore,we suggest that the recovered caryopses were formed at about 250°C by baking rather than by burning in an open fire. | YANG Qing LI XiaoQiang ZHOU XinYing ZHAO KeLiang JI Ming SUN Nan | 2011 | Chinese Science Bulletin2011，56，14： | 10 |
| 7 | Strategies to anode protection in lithium metal battery:A review显示文摘Lithium metal batteries(LMBs)are considered the most promising energy stor-age devices for applications such as electrical vehicles owing to its tremendous theoretical capacity(3860 mAh g−1).However,the serious safety issues and poor cycling performance caused by the dendritic crystal growth during deposi-tion are concerned for any rechargeable batteries with a lithium metal anode.To make widespread adoption a possibility,considerable efforts have been devoted to suppressing lithium(Li)dendrite growth.In this review,the recent strategies to developing dendrite free Li anode,including constructing an artifi-cial solid electrolyte interface,current collector modification,separator film improvement,and electrolyte additive,are summarized.The merits and short-comings for different strategies are reviewed and a general summary and per-spective on the next generation rechargeable batteries are presented. | Jiawei Li Zhao Kong Xiaoxi Liu Bocong Zheng Qi Hua Fan Elias Garratt Thomas Schuelke Keliang Wang Hui Xu Hong Jin | 2021 | InfoMat2021，3，12： | 8 |
| 8 | Mitochondrial replacement by pre-pronuclear transfer in human embryos显示文摘 | Wu, Keliang Chen, Tailai Huang, Sexin Zhong, Cuiqing Yan, Junhao Zhang, Xiaoyu Li, Jinsong Gao, Yuan Zhao, Han Chen, Zi-Jiang | 2017 | Cell Research2017，27，6： | 7 |
| 9 | Holocene vegetation succession and responses to climate change in the northern sector of Northeast China显示文摘Sediment pollen samples from the Huola Basin in the northern sector of northeast China, and surface pollen samples from its environs, were analyzed to reconstruct accurately the historical response of vegetation to climate change since 9100 cal yr BP. Pollen analysis of the Huola Section indicates that vegetation experienced a transformation from early-mid Holocene warm-cold mixed vegetation to late Holocene cold-temperate vegetation. From 9100 to 6000 cal yr BP, the study area was warmer and moister than at present, developing Corylus, Carpinus, Pinus, Picea, Betula and Larix-dominated forests. Two cooling events at 6000–5000 and 3500–2500 cal yr BP led to a decrease in Corylus, Carpinus and other warmth-loving vegetation, whereas cold temperate forests composed of Larix and Betula expanded. After 2500 cal yr BP, Larix and Betula dominated cold-temperate vegetated landscapes. The Holocene warm period in NE China(9100–6000 cal yr BP) suggests that such warming could have resulted in a strengthening of the influence from East Asian Summer Monsoon on northernmost NE China and would have benefited the development of warm-temperate forest vegetation and an improved plant load, which also provides the similarity model for the possible global warming in the future. | ZHAO Chao LI XiaoQiang ZHOU XinYing ZHAO KeLiang YANG Qing | 2016 | Science China Earth Sciences2016，59，7： | 7 |
| 10 | Quick repositioning maneuver for horizontal semicircular canal benign paroxysmal positional vertigo显示文摘Objective:To investigate the efficacy of quick repositioning maneuver for horizontal semicircular canal benign paroxysmal positional vertigo(H-BPPV).Methods:Clinical data of 67 patients with H-BPPV who underwent quick repositioning maneuver in our hospital from July 2009 to November2014 were retrospectively analyzed.The maneuver involved rotating the patient in the axial plane for 180 from the involved side towards contralateral side as quickly as possible.Results:Complete symptom resolution was achieved in 61 patients(91.0%) at one week and in 64 patients(95.5%) at 3 months post-treatment.During the repositioning maneuver process,there were no obvious untoward responses except transient nausea with or without vomiting in a few patients.Conclusion:The results indicate that the quick repositioning maneuver is an easy and effective alternative treatment in the management of HBPPV. | Jinrang Li Pengfei Guo Shiyu Tian Keliang Li Hao Zhang | 2015 | Journal of Otology2015，10，3： | 6 |
| 11 | Extractive Distillation of Methyl Acetate-Methanol Azeotrope Using [DMIM]DMP as Solvent显示文摘In order to separate methyl acetate and methanol azeotrope, the ionic liquid(IL) 1,3-dimethylimidazolium dimethylphosphate([DMIM]DMP) was used as the solvent. The Aspen Plus software was used to design and optimize the extractive distillation process. Under the optimized conditions, the mass fractions of methyl acetate and methanol were both above 99.5%. Compared with the dimethyl sulfoxide(DMSO) process, the [DMIM]DMP process has the advantages of saving energy and lower equipment investment cost. The result shows that using [DMIM]DMP as the solvent to separate a methyl acetate and methanol azeotrope has better prospect in industrial application. | Li Jing Wang Keliang Lian Minglei Li Zhi Du Tingzhao | 2018 | China Petroleum Processing & Petrochemical Technology2018，20，4： | 6 |
| 12 | An App knock-in rat model for Alzheimer’s disease exhibiting Aβand tau pathologies,neuronal death and cognitive impairments显示文摘A major obstacle in Alzheimer’s disease(AD)research is the lack of predictive and translatable animal models that reflect disease progression and drug efficacy.Transgenic mice overexpressing amyloid precursor protein(App)gene manifest non-physiological and ectopic expression of APP and its fragments in the brain,which is not observed in AD patients.The App knock-in mice circumvented some of these problems,but they do not exhibit tau pathology and neuronal death.We have generated a rat model,with three familiar App mutations and humanized Aβsequence knocked into the rat App gene.Without altering the levels of full-length APP and other APP fragments,this model exhibits pathologies and disease progression resembling those in human patients:deposit of Aβplaques in relevant brain regions,microglia activation and gliosis,progressive synaptic degeneration and AD-relevant cognitive deficits.Interestingly,we have observed tau pathology,neuronal apoptosis and necroptosis and brain atrophy,phenotypes rarely seen in other APP models.This App knock-in rat model may serve as a useful tool for AD research,identifying new drug targets and biomarkers,and testing therapeutics. | Keliang Pang Richeng Jiang Wei Zhang Zhengyi Yang Lin-Lin Li Makoto Shimozawa Simone Tambaro Johanna Mayer Baogui Zhang Man Li Jiesi Wang Hang Liu Ailing Yang Xi Chen Jiazheng Liu Bengt Winblad Hua Han Tianzi Jiang Weiwen Wang Per Nilsson Wei Guo Bai Lu | 2022 | Cell Research2022，32，2： | 6 |
| 13 | Pantograph-catenary electrical contact system of high-speed railways:recent progress,challenges,and outlooks显示文摘As the unique power entrance,the pantograph-catenary electrical contact system maintains the efficiency and reliability of power transmission for the high-speed train.Along with the fast development of high-speed railways all over the world,some commercialized lines are built for covering the remote places under harsh environment,especially in China;these environmental elements including wind,sand,rain,thunder,ice and snow need to be considered during the design of the pantograph-catenary system.The pantograph-catenary system includes the pantograph,the contact wire and the interface—pantograph slide.As the key component,this pantograph slide plays a critical role in reliable power transmission under dynamic condition.The fundamental material characteristics of the pantograph slide and contact wire such as electrical conductivity,impact resistance,wear resistance,etc.,directly determine the sliding electrical contact performance of the pantograph-catenary system;meanwhile,different detection methods of the pantograph-catenary system are crucial for the reliability of service and maintenance.In addition,the challenges brought from extreme operational conditions are discussed,taking the Sichuan-Tibet Railway currently under construction as a special example with the high-altitude climate.The outlook for developing the ultra-high-speed train equipped with the novel pantograph-catenary system which can address the harsher operational environment is also involved.This paper has provided a comprehensive review of the high-speed railway pantograph-catenary systems,including its progress,challenges,outlooks in the history and future. | Guangning Wu Keliang Dong Zhilei Xu Song Xiao Wenfu Wei Huan Chen Jie Li Zhanglin Huang Jingwei Li Guoqiang Gao Guozheng Kang Chuanjun Tu Xingyi Huang | 2022 | Railway Engineering Science2022，30，4： | 5 |
| 14 | Neural network-based matrix effect correction in EDXRF analysis显示文摘In this paper we discuss neural network-based matrix effect correction in energy dispersive X-ray fluorescence (EDXRF) analysis,with detailed algorithm to classify the samples.The method can correct the matrix effect effectively through classifying the samples automatically,and influence of X-ray absorption and enhancement by major elements of the samples is reduced.Experiments for the complex matrix effect correction in EDXRF analysis of samples in Pangang showed improved accuracy of the elemental analysis result. | TUO Xianguo CHENG Bo MU Keliang LI Zhe | 2008 | Nuclear Science and Techniques2008，19，5： | 4 |
| 15 | MC simulation of a PGNAA system for on-line cement analysis显示文摘A prompt gamma neutron activation analysis system with a 252Cf neutron source for on-line cement analysis has been simulated with the MCNP code.The results indicate that the optimum arrangement is a Bi shield of 20-mm thickness,a polyethylene moderator of 50-mm thickness,a source-to-sample distance of 70 mm,and cement samples of 1200 mm×600 mm×170 mm.To absorb thermal neutrons and suppress low-energy γ-rays,the optimum-sized sheets are 150 mm×7 mm Cd,and 150 mm×15 mm Pb. | YANG Jianbo TUO Xianguo LI Zhe MU Keliang CHENG Yi MOU Yunfeng | 2010 | Nuclear Science and Techniques2010，21，4： | 4 |
| 16 | Monte-Carlo simulation of cement neutron field distribution characteristics in PGNAA显示文摘The distribution characteristics of the neutron field in cement was simulated using the MCNP code to comply with the requirements of an online Prompt Gamma Neutron Activation Analysis system.Simulation results showed that the neutron relative flux proportion reduced with increasing cement thickness.When the cement thickness remains unchanged,the reduced proportion of thermal neutrons increases to a small extent,but the epithermal, intermediate,and fast neutrons will decrease according to the geometric progression.H element in the cement mainly affects the reduction of fast neutrons and other single-substance elements,e.g.,O,Ca,56Fe,Si,and Al.It also slows down the reduction of the fast neutrons via inelastic scattering.O contributes more than other elements in the reduction of fast neutrons.Changing the H content affects the thermal,epithermal,intermediate,and fast neutrons, while changing the Ca,Fe,and Si contents only influences the thermal,epithermal,and intermediate neutrons;hence, there is little effect on the reduction of fast neutrons. | YANG Jianbo YANG Yigang LI Yuanjing TUO Xianguo LI Zhe LIU Mingzhe CHENG Yi MU Keliang WANG Lei | 2012 | Nuclear Science and Techniques2012，23，6： | 3 |
| 17 | In vitro expansion of human sperm through nuclear transfer显示文摘Dear Editor,Mammalian haploid embryonic stem cells(haESCs)represent an ideal tool for genetic analysis due to the presence of only one set of genetic materials.HaESCs fall into two readily distinguishable groups based on their genome origins:parthenogenetic haESCs(PG-haESCs)that are derived from oocyte-originated parthenogenetic embryos and androgenetic haESCs(AG-haESCs)that are produced through sperm nuclear transfer.Both PG-and AGhaESCs are feasible for delineating genome function at cellular level in vitro.Importantly,AG-haESCs can be used as a sperm replacement and applied to deciphering gene function at organismal level when combined with CRISPR-Cas9 technology.1 However,while PG-haESCs have been generated from both rodent and primates,2-5 AG-haESCs can only be obtained in rodent to date. | Xiaoyu Merlin Zhang Keliang Wu Yuxuan Zheng Han Zhao Junpeng Gao Zhenzhen Hou Meiling Zhang Jiaoyang Liao Jingye Zhang Yuan Gao Yuanyuan Li Lin Li Fuchou Tang Zi-Jiang Chen Jinsong Li | 2020 | Cell Research2020，30，4： | 3 |
| 18 | Rhodojaponin Ⅵ indirectly targets Cav2.2 channels via N-ethylmaleimide-sensitive fusion protein to alleviate neuropathic pain显示文摘Neuropathic pain is a chronic disease that severely afflicts the life and emotional status of patients,but currently available treatments are often ineffective.Novel therapeutic targets for the alleviation of neuropathic pain are urgently needed.Rhodojaponin Ⅵ,a grayanotoxin from Rhododendron molle,showed remarkable antinociceptive efficacy in models of neuropathic pain,but its biotargets and mechanisms are unknown.Given the reversible action of rhodojaponin Ⅵ and the narrow range over which its structure can be modified,we perforwmed thermal proteome profiling of the rat dorsal root ganglion to determine the protein target of rhodojaponin Ⅵ.N-Ethylmaleimide-sensitive fusion(NSF) was confirmed as the key target of rhodojaponin Ⅵ through biological and biophysical experiments.Functional validation showed for the first time that NSF facilitated trafficking of the Cav2.2 channel to induce an increase in Ca2+current intensity,whereas rhodojaponin Ⅵ reversed the effects of NSF.In conclusion,rhodojaponin Ⅵ represents a unique class of analgesic natural products targeting Cav2.2 channels via NSF. | Keliang Chen Tao Wang Yong Li Jun Wu Cheng-Xiao Zhao Sheng Liu Fengrun Sun Yehong Fang Jiahuan Hu Jinping Hu Chong-Jing Zhang Haibo Yu Chao Ma Shi-Shan Yu | 2023 | Acta Pharmaceutica Sinica B2023，13，3： | 2 |
| 19 | A Role for Transmembrane Protein 16C/Slack Impairment in Excitatory Nociceptive Synaptic Plasticity in the Pathogenesis of Remifentanil-induced Hyperalgesia in Rats显示文摘Remifentanil is widely used to control intraoperative pain. However, its analgesic effect is limited by the generation of postoperative hyperalgesia. In this study, we investigated whether the impairment of transmembrane protein 16C(TMEM16C)/Slack is required for a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic receptor(AMPAR) activation in remifentanil-induced postoperative hyperalgesia. Remifentanil anesthesia reduced the paw withdrawal threshold from 2 h to 48 h postoperatively,with a decrease in the expression of TMEM16C and Slack in the dorsal root ganglia(DRG) and spinal cord.Knockdown of TMEM16C in the DRG reduced the expression of Slack and elevated the basal peripheral sensitivity and AMPAR expression and function. Overexpression of TMEM16C in the DRG impaired remifentanilinduced ERK1/2 phosphorylation and behavioral hyperalgesia. AMPAR-mediated current and neuronal excitability were downregulated by TMEM16C overexpression in the spinal cord. Taken together, these findings suggest that TMEM16C/Slack regulation of excitatory synaptic plasticity via GluA1-containing AMPARs is critical in the pathogenesis of remifentanil-induced postoperative hyperalgesia in rats. | Yize Li Linlin Zhang Jing Li Chunyan Wang Yi Chen Yuan Yuan Keliang Xie Guolin Wang Yonghao Yu | 2021 | Neuroscience Bulletin2021，37，5： | 2 |
| 20 | Query Expansion Based on Semantics and Statistics in Chinese Question Answering System显示文摘In Chinese question answering system, because there is more semantic relation in questions than that in query words, the precision can be improved by expanding query while using natural language questions to retrieve documents. This paper proposes a new approach to query expansion based on semantics and statistics.Firstly automatic relevance feedback method is used to generate a candidate expansion word set. Then the expanded query words are selected from the set based on the semantic similarity and seman-tic relevancy between the candidate words and the original words. Experiments show the new approach is effective for Web retrieval and out-performs the conventional expansion approaches. | JIA Keliang PANG Xiuling LI Zhinuo FAN Xiaozhong | 2008 | Wuhan University Journal of Natural Sciences2008，13，4： | 2 |
您所在的机构暂未开通此部分的功能，请联系管理员