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| 1 | The Chromosome-Level Reference Genome of Tea Tree Unveils Recent Bursts of Nonautonomous LTR Retrotransposons in Driving Genome Size Evolution显示文摘Dear Editor,The tea tree Camellia sinensis,a member of the genus Camellia in the Theaceae family,includes two major cultivated varieties,C.sinensis var.assamica(CSA\Assam type)and C.sinensis var.sinensis(CSS;Chinese type)(Ming and Bartholomew,2007).Due to the high economic importance of the tea tree,considerable efforts have been made to explore genetic basis of the biosynthesis of natural metabolites that determine health benefits and diverse tea flavors(Shi et al.,2011;Li et al.,2011;Li et al.,2015;Xia et aL,2017;Liu et al.,2019). | Qun-Jie Zhang Wei L i Kui Li Hong Nan Cong Shi Yun Zhang Zhang-Yan Dai Yang-Lei Lin Xiao-Lan Yang Yan Tong Dan Zhang Cui Lu Li-Ying Feng Chen-Feng Wang Xiao-Xin Liu Jian-An Huang Wen-Kai Jiang Xing-Hua Wang Xing-Cai Zhang Evan E.Eichler Zhong-Hua Liu and Li-Zhi Gao | 2020 | Molecular Plant2020，13，7： | 27 |
| 2 | A Prospective Randomized Multicenter Controlled Trial on Salvianolate for Treatment of Unstable Angina Pectoris in A Chinese Elderly Population显示文摘Objective: To evaluate the efficacy and safety of salvianolate in elderly patients with unstable angina pectoris(UAP). Methods: A prospective double-blind randomized placebo-controlled multicenter trial in elderly patients with UAP from 13 third-grade class-A hospitals in China was performed. A total of 318 patients were randomly allocated in a 1:1 ratio to an experimental group(160 patients) and a control group(158 patients). The experimental group was treated with salvianolate for 14 days on the basis of conventional medicine, and the control group was given a placebo for 14 days with the same criteria. Follow-up was lasted 28 days in both groups. The primary endpoint was biweekly frequency of angina pectoris attacks. The secondary endpoints included biweekly dosage of nitroglycerin, the Seattle Angina Questionnaire, angina pectoris severity and duration, myocardial injury markers, high-sensitivity C-reactive protein(hs-CRP) and N-terminal pro-B-type natriuretic peptide(NT-proBNP), as well as major adverse cardiovascular events(MACEs). Safety was assessed according to adverse events and serious adverse events. Results: Baseline characteristics were similar between treatment groups. Compared with those in the control group, the frequency of biweekly angi na attacks(2.92 vs. 4.08, P =0.025), th e biweekly dosage of nitroglycerin, as well as the severity and duration of angina attacks(P <0.01) were reduced by salvianolate. The Seattle Angina Questionnaire score was also significantly improved in the experimental group than in the control group(P <0.05). No significant differences were observed between the two groups with respect to the incidence of MACEs. Salvianolate was well tolerated. Conclusions: Salvianolate appear to have efficacy and well tolerated for elderly patients with UAP. | CUI Hua LI Xiao-ying GAO Xue-wen LU Xiang WU Xiu-ping WANG Xiao-fei ZHENG Xiang-qing HUANG Kui LIU Feng LUO Zhi YUAN Hui-shu SUN Gang KONG Jian DU Xiao-hong ZHENG Jin LIU Hong-ying ZHANG Wen-ju | 2019 | Chinese Journal of Integrative Medicine2019，25，10： | 13 |
| 3 | IL-37b gene transfer enhances the therapeutic efficacy of mesenchumal stromal cells in DSS- induced colitis mice显示文摘瞄准:调查是否 IL-37b 基因的转移,天生、适应的免疫的一个最新识别的禁止者,能在煽动性的肠疾病( IBD )改进 mesenchumal stromal 房间( MSC )的治疗学的功效 .Methods :在有活跃 ulcerative 大肠炎( UC )的病人的 biopsied 标本的 IL-37 的表示用 RT-PCR 和 immunohistochemistry 被检测。老鼠与 3% 葡聚糖硫酸盐钠(决策支持系统) 被对待 8 天导致大肠炎。在决策支持系统治疗前,老鼠与 MSC, MSC-eGFP 或 MSC-IL37b 被注射。他们的身体重量每天被测量,并且结肠和脾在 d 上被收获 10 为病理学、生物化学的 analyses.Results : 在有活跃 UC 的病人,的 biopsied 标本, IL-37 的表示戏剧性地在煽动的 mucosa 被提高,主要在上皮的房间和渗透的有免疫力的房间。比作 MSC-eGFP 或 MSC, MSC-IL37b 管理显著地稀释了身体重量和冒号长度减小,并且减少在导致决策支持系统的大肠炎老鼠的组织学的分数。而且, MSC-IL37b 管理在老鼠在脾的 CD4+ T 房间之中象规章的 T 房间(Tregs ) 的百分比一样在全部的脾的 mononuclear 房间之中增加了导出 myeloid 的 suppressor 房间(MDSC ) 的百分比。而且, MSC-IL37b 管理增加了 IL-2+ 房间并且减少 IFN-γ在脾的 CD4+ T cells.Conclusion 之中的 + 房间: IL-37 涉及 UC 的 pathophysiology。IL-37b 基因转移由导致 Tregs 和 MDSC 并且调整 cytokine 生产在导致决策支持系统的大肠炎老鼠提高 MSC 的治疗学的功效。 | Wei-qiang WANG Kui DONG Lu ZHOU Guo-hui JIAO Cong-zhong ZHU Wen-wen LI Gang YU Wan-tong WU Song CHEN Zhi-na SUN Yu-ming WANG Wen-tian LIU Jie ZHANG Bang-mao WANG Xiao-ming FENG | 2015 | Acta Pharmacologica Sinica2015，36，11： | 13 |
| 4 | IL-37b suppresses T cell priming by modulating dendritic cell maturation and cytokine production via dampening ERK/NF-κB/S6K signalings显示文摘Interleukin 37b (IL-37b ) 在压制有免疫力的回答起一个关键作用,部分由 modulating 树枝状的房间(DC ) 的功能。然而,精确机制大部分仍然是未知的。这里,我们在回答由 DC 导致了的 DC 成熟和 T 房间上调查了 IL-37b 的效果,并且探索了深奥发信号的小径。它被发现那 IL-37b 下面调整在 vitro 的 DC 上的 co-stimulatory 分子 CD80 和 CD86 的表情。同时,支持 inflammatory cytokines 的表情例如 TNF-6, 和 IL-6,被压制,当时 T 房间的表示禁止的 cytokine TGF- 在 IL-37b-treated DC 被增加。另外, T 房间上的 DC 的激活效果被 IL-37b 损害。我们进一步表明细胞外的调整单人赛的 kinase (英皇家空军之阶级最低之兵) ,原子 factor-B (NF-B ) ,和表明小径的 mTOR-S6K 涉及 IL-37b 导致的 DC 的抑制。这被化学禁止者的同样镇压的效果在 DC 在 IL-6 和 TNF- 的表情上对 NF-B,英皇家空军之阶级最低之兵,和 S6K 证实。在结论,这些结果证明 IL-37b 由在英皇家空军之阶级最低之兵, NF-B,和基于 S6K 的禁止的发信号小径包含在 T 房间 priming 压制了 DC 成熟和 immunostimulatory 能力。 | Wantong Wu Weiqiang Wang Yun Wang Wenwen Li Gang Yu Zhonglong Li Chunmin Fang Yue Shen Zhina Sun Ling Han Juan Yu Lijun Fang Song Chen Kui Dong Zhongchao Han Hanzhi Liu Yuechen Luo Xiaoming Feng | 2015 | Acta Biochimica et Biophysica Sinica2015，47，8： | 8 |
| 5 | A phenolic glycoside from Fagopyrum dibotrys (D.Don) Hara显示文摘A new phenolic constituent along with five known compounds were isolated from Fagopyrum dibotrys(D.Don)Hara.The new compound was characterized as 1,3-dimethoxy-2-O-β-xylo-pyranosyl-5-O-β-glucopyranosyl-benzene,by spectroscopic analysis and enzymatic hydrolysis. | Zheng Zhong Bai Xiao Hui Zhang Li Jiang Xuan Feng Kui Mo | 2007 | Chinese Chemical Letters2007，18，9： | 8 |
| 6 | Model Predictive Current Control with Adaptive-adjusting Timescales for PMSMs显示文摘A model predictive current control(MPCC)with adaptive-adjusting method of timescales for permanent magnet synchronous motors(PMSMs)is proposed in this paper to improve the dynamic response and prediction accuracy in transient-state,while lessening the computational burden and improving the control performance in steady-state.The timescale characteristics of different parts of MPCC,such as signal sampling,prediction calculation,control output,model error correction,are analyzed,and the algorithm architecture of MPCC with multi-timescale is proposed.The difference between reference and actual speed,and the change rate of actual speed are utilized to discriminate the transient state of speed change and load change,respectively.Adaptive-adjusting method of control period and prediction stepsize are illustrated in detail after operation condition discrimination.Experimental results of a PMSM are presented to validate the effectiveness of proposed MPCC.In addition,comparative evaluation of single-step MPCC with fixed timescale and proposed MPCC is conducted,which demonstrates the superiority of proposed control strategy. | Feng Niu Xi Chen Shaopo Huang Xiaoyan Huang Lijian Wu Kui Li Youtong Fang | 2021 | CES Transactions on Electrical Machines and Systems2021，5，2： | 8 |
| 7 | Effect of Lactobacillus rhamnosus GG supernatant on serotonin transporter expression in rats with post-infectious irritable bowel syndrome显示文摘AIM To evaluate the effect of Lactobacillus rhamnosus GG supernatant(LGG-s) on the expression of serotonin transporter(SERT) in rats with post-infectious irritable bowel syndrome(PI-IBS).METHODS Campylobacter jejuni 81-176(1010 CFU/m L) was used to induce intestinal infection to develop a PI-IBS model. After evaluation of the post-infectious phase by biochemical tests, Dn A agarose gel electrophoresis, abdominal withdrawal reflex(AWR) test, and the intestinal motility test, four PI-IBS groups received different concentrations of LGG-s for 4 wk. The treatments were maintained for 1.0, 2.0, 3.0 or 4.0 wk during the experiment, and the colons and brains were removed for later use each week. SERT m Rn A and protein levels were detected by real-time PCR and Western blot, respectively.RESULTS The levels of SERT m Rn A and protein in intestinal tissue were higher in rats treated with LGG-s than in control rats and PI-IBS rats gavaged with PBS during the whole study. Undiluted LGG-s up-regulated SERT m Rn A level by 2.67 times compared with the control group by week 2, and SERT m Rn A expression kept increasing later. Double-diluted LGG-s was similar to undiluted-LGG-s, resulting in high levels of SERT m Rn A. Triple-diluted LGG-s up-regulated SERT m Rn A expression level by 6.9-times compared with the control group, but SERT m Rn A expression decreased rapidly at the end of the second week. At the first week, SERT protein levels were basically comparable in rats treated with undiluted LGG-s, double-diluted LGG-s, and triplediluted LGG-s, which were higher than those in the control group and PBS-treated PI-IBS group. SERT protein levels in the intestine were also comparable in rats treated with undiluted LGG-s, double-diluted LGG-s, and triple-diluted LGG-s by the second and third weeks. SERT m Rn A and protein levels in the brain had no statistical difference in the groups during the experiment.CONCLUSION LGG-s can up-regulate SERT m Rn A and protein levels in intestinal tissue but has no influence in brain tissue in rats with PI-IBS. | Ya-nan Cao Li-juan Feng Yuan-Yuan Liu Kui jiang Mao-jun Zhang Yi-xin Gu Bang-Mao Wang jia Gao Ze-Lan Wang Yu-Ming Wang | 2018 | World Journal of Gastroenterology2018，24，3： | 7 |
| 8 | Vacancy formation enthalpies of high-entropy FeCoCrNi alloy via first-principles calculations and possible implications to its superior radiation tolerance显示文摘Because atoms in high-entropy alloys(HEAs) coordinate in very different and distorted local environments in the lattice sites, even for the same type of constituent, their point defects could highly vary.Therefore, theoretical determination of the thermodynamic quantities(i.e., defect formation enthalpies)of various point defects is rather challenging because each corresponding thermodynamic quantity of all involve constituents is not unique. The knowledge of these thermodynamic quantities is prerequisite for designing novel HEAs and understanding the mechanical and physical behaviors of HEAs. However,to date there has not been a good method to theoretically derive the defect formation enthalpies of HEAs. Here, using first-principles calculations within the density functional theory(DFT) in combination of special quasi-random structure models(SQSs), we have developed a general method to derive corresponding formation enthalpies of point defects in HEAs, using vacancy formation enthalpies of a four-component equiatomic fcc-type FeCoCrNi HEA as prototypical and benchmark examples. In difference from traditional ordered alloys, the vacancy formation enthalpies of FeCoCrNi HEA vary in a highly wide range from 0.72 to 2.89 eV for Fe, 0.88–2.90 eV for Co, 0.78–3.09 eV for Cr, and 0.91–2.95 eV for Ni due to high-level site-to-site lattice distortions and compositional complexities. On average, the vacancy formation enthalpies of 1.58 eV for Fe, 1.61 eV for Cr, 1.70 eV for Co and 1.89 eV for Ni are all larger than that(1.41 eV) of pure fcc nickel. This fact implies that the vacancies are much more difficult to be created than in nickel, indicating a reasonable agreement with the recent experimental observation that FeCoCrNi exhibits two orders of amplitudes enhancement of radiation tolerance with the suppression of void formation at elevated temperatures than in pure nickel. | Weiliang Chen Xueyong Ding Yuchao Feng Xiongjun Liu Kui Liu Z.P. Lu Dianzhong Li Yiyi Li C.T. Liu Xing-Qiu Chen | 2018 | Journal of Materials Science & Technology2018，34，2： | 6 |
| 9 | Suppressor of cytokine signaling 1 protects rat pancreatic islets from cytokine-induced apoptosis through Janus kinase/signal transducers and activators of transcription pathway显示文摘 | Sun Qi Xiang Ruo-lan Yang Yan-li Feng Kai Zhang Kui Ding Wen-yi | 2013 | Chinese Medical Journal2013，，21： | 6 |
| 10 | Gapless indica rice genome reveals synergistic contributions of active transposable elements and segmental duplications to rice genome evolution显示文摘The ultimate goal of genome assembly is a high-accuracy gapless genome.Here,we report a new assembly pipeline that is used to produce a gapless genome for the indica rice cultivar Minghui 63.The resulting 397.71-Mb final assembly is composed of 12 contigs with a contig N50 size of 31.93 Mb.Each chromosome is represented by a single contig and the genomic sequences of all chromosomes are gapless.Quality evaluation of this gapless genome assembly showed that gene regions in our assembly have the highest completeness compared with the other 15 reported high-quality rice genomes.Further comparison with the japonica rice genome revealed that the gapless indica genome assembly contains more transposable elements(TEs)and segmental duplications(SDs),the latter of which produce many duplicated genes that can affect agronomic traits through dose effect or sub-/neo-functionalization.The insertion of TEs can also affect the expression of duplicated genes,which may drive the evolution of these genes.Furthermore,we found the expansion of nucleotide-binding site with leucine-rich repeat disease-resistance genes and cis-zeatin-O-glucosyltransferase growth-related genes in SDs in the gapless indica genome assembly,suggesting that SDs contribute to the adaptive evolution of rice disease resistance and developmental processes.Collectively,our findings suggest that active TEs and SDs synergistically contribute to rice genome evolution. | Kui Li Wenkai Jiang Yuanyuan Hui Mengjuan Kong Li-Ying Feng Li-Zhi Gao Pengfu Li Shan Lu | 2021 | Molecular Plant2021，14，10： | 6 |
| 11 | new anastomosis method for choledochojejunostomy by the way behind antrue pyloricum显示文摘 | YANG Xin-wei YANG Jue WANG Kui ZHANG Bao-hua SHEN Feng WU Meng-chao | 2013 | Chinese Medical Journal2013，，24： | 5 |
| 12 | Cancer metabolism and tumor microenvironment:fostering each other?显示文摘The changes associated with malignancy are not only in cancer cells but also in environment in which cancer cells live.Metabolic reprogramming supports tumor cells’high demand of biogenesis for their rapid proliferation,and helps tumor cells to survive under certain genetic or environmental stresses.Emerging evidence suggests that metabolic alteration is ultimately and tightly associated with genetic changes,in particular the dysregulation of key oncogenic and tumor suppressive signaling pathways.Cancer cells activate HIF signaling even in the presence of oxygen and in the absence of growth factor stimulation.This cancer metabolic phenotype,described firstly by German physiologist Otto Warburg,ensures enhanced glycolytic metabolism for the biosynthesis of macromolecules.The conception of metabolite signaling,i.e.,metabolites are regulators of cell signaling,provides novel insights into how reactive oxygen species(ROS)and other metabolites deregulation may regulate redox homeostasis,epigenetics,and proliferation of cancer cells.Moreover,the unveiling of noncanonical functions of metabolic enzymes,such as the moonlighting functions of phosphoglycerate kinase 1(PGK1),reassures the importance of metabolism in cancer development.The metabolic,microRNAs,and ncRNAs alterations in cancer cells can be sorted and delivered either to intercellular matrix or to cancer adjacent cells to shape cancer microenvironment via media such as exosome.Among them,cancer microenvironmental cells are immune cells which exert profound effects on cancer cells.Understanding of all these processes is a prerequisite for the development of a more effective strategy to contain cancers. | Yiyuan Yuan Huimin Li Wang Pu Leilei Chen Dong Guo Hongfei Jiang Bo He Siyuan Qin Kui Wang Na Li Jingwei Feng Jing Wen Shipeng Cheng Yaguang Zhang Weiwei Yang Dan Ye Zhimin Lu Canhua Huang Jun Mei Hua-Feng Zhang Ping Gao Peng Jiang Shicheng Su Bing Sun Shi-Min Zhao | 2022 | Science China(Life Sciences)2022，65，2： | 5 |
| 13 | Synthesis of Dimethyl Carbonate from Methanol and Carbon Dioxide Catalyzed by Potassium Hydroxide under Mild Conditions显示文摘The synthesis of dimethyl carbonate (DMC) from methanol and carbon dioxide using potassium hydroxide as catalyst in the presence of CH3I and the effect of ionic liquid on the reaction were investigated. The results showed that KOH is an effective catalyst; the high selectivity and raised yield of DMC formation under mild conditions were achieved. However, the addition of the ionic liquid, 1-ethyl-3-methylimidazolium bromide (emimBr), can evidently accelerate the conversion of methanol and yield of the product. | Hong WANG Bin LU Qing Hai CAI Feng WU Yong Kui SHAN | 2005 | Chinese Chemical Letters2005，16，9： | 5 |
| 14 | Patients with advanced primary hepatocellular carcinoma treated by melatonin and transcatheter arterial chemoembolization: a prospective study显示文摘Objective: To observe the clinical efficacy of tran-scatheter arterial chemoembolization (TACE) andTACE+MLT (melatonin) on inoperable advancedprimary hepatocellular carcinoma.Methods: From January 1997 to January 1998, onehundred patients with inoperable advanced primaryhepatocellular carcinoma were treated separately byTACE (50) and TACE+MLT (20 mg/d at 8:00PM orally, 7 days before TACE) (50).Results: The effective rates (WHO standards) ofTACE and TACE+MLT were 16% and 28% respec-tively (P<0.05). After TACE or TACE+MLT,the resection rate at two-stage of TACE was 4% or14% (P<0. 01). The 0.5-, 1- and 2-year survivalrates in the TACE group were 82%, 54% and 26%respectively; in the TACE+MLT group 100%, 68%and 40% respectively. The results were significantlybetter in the TACE+MLT group than in the TACEgroup. MLT could protect liver function from thedamage caused by TACE. The IL-2 levels of all pa-tients significantly increased, whereas sIL-2R expres-sions decreased after TACE+MLT as compared withthe TACE group (P<0.01).Conclusions: With definite protection and treatmenteffect on the liver function damage caused by TACE,MLT can enhance the immunological activities of pa-tients. It also can improve the effect of TACE by in-creasing the survival and resection rate after two-stage operation. | Jian-Jun Yan Feng Shen Kui Wang Meng-Chao Wu From the Eastern Hepatobiliary Surgrry Hospital, Shanghai 200438, China | 2002 | Hepatobiliary & Pancreatic Diseases International2002，1，2： | 5 |
| 15 | Molecular Epidemiology of Coxsackievirus B1-5 Associated with HFMD in Fujian Province, China, 2011-2016显示文摘Hand,foot and mouth disease(HFMD)is a common infectious disease that usually affects children less than 5 years of age.HFMD is caused by human enteroviruses(HEVs).HEVs,members of the Enterovirus genus of the Picornaviridae(small RNA virus)family. | CHEN Wei WENG Yu Wei ZHANG Yong Jun HE Wen Xiang ZHU Ying YU Ting Ting XIE Jian Feng ZHENG Kui Cheng YAN Yan Sheng ZHANG Wen Chang | 2019 | Biomedical and Environmental Sciences2019，32，8： | 4 |
| 16 | RT-nPCR Assays for Amplification and Sequencing of VP1 Genes in Human Enterovirus A–D from Clinical Specimens显示文摘Objective To develop RT-nPCR assays for amplifying partial and complete VP1 genes of human enteroviruses(HEVs)from clinical samples and to contribute to etiological surveillance of HEV-related diseases.Methods A panel of RT-nPCR assays,consisting of published combined primer pairs for VP1 genes of HEV A–C and in-house designed primers for HEV-D,was established in this study.The sensitivity of each RT-nPCR assay was evaluated with serially diluted virus stocks of five serotypes expressed as CCID50 perμL and copies perμL,and the newly established methods were tested in clinical specimens collected in recent years.Results The sensitivity of RT-nPCR assays for amplifying partial VP1 gene of HEVs was 0.1 CCID50 perμL and 10 virus copies perμL,and for the complete VP1 gene was 1 CCID50 perμL and 100 virus copies perμL,using serially-diluted virus stocks of five serotypes.As a proof-of-concept,25 serotypes were identified and complete VP1 sequences of 23 serotypes were obtained by this system among 858 clinical specimens positive for HEVs during the past eight surveillance seasons.Conclusion This RT-nPCR system is capable of amplifying the partial and complete VP1 gene of HEV A–D,providing rapid,sensitive,and reliable options for molecular typing and molecular epidemiology of HEVs in clinical specimens. | CHEN Wei WENG Yu Wei HE Wen Xiang ZHU Ying YU Ting Ting XIE Jian Feng ZHENG Kui Cheng YAN Yan Sheng ZHANG Yong Jun ZHANG Wen Chang | 2020 | Biomedical and Environmental Sciences2020，33，11： | 4 |
| 17 | Management of regional citrate anticoagulation for continuous renal replacement therapy:guideline recommendations from Chinese emergency medical doctor consensus显示文摘Continuous renal replacement therapy(CRRT)is widely used for treating critically-ill patients in the emergency department in China.Anticoagulant therapy is needed to prevent clotting in the extracorporeal circulation during CRRT.Regional citrate anticoagulation(RCA)has been shown to potentially be safer and more effective,and is now recommended as the preferred anticoagulant method for CRRT.However,there is still a lack of unified standards for RCA management in the world,and there are many problems in using this method in clinical practice.The Emergency Medical Doctor Branch of the Chinese Medical Doctor Association(CMDA)organized a panel of domestic emergency medicine experts and international experts of CRRT to discuss RCA-related issues,including the advantages and disadvantages of RCA in CRRT anticoagulation,the principle of RCA,parameter settings for RCA,monitoring of RCA(mainly metabolic acid-base disorders),and special issues during RCA.Based on the latest available research evidence as well as the paneled experts'clinical experience,considering the generalizability,suitability,and potential resource utilization,while also balancing clinical advantages and disadvantages,a total of 16 guideline recommendations were formed from the experts'consensus. | Shu-Yuan Liu Sheng-Yong Xu Lu Yin Ting Yang Kui Jin Qiu-Bin Zhang Feng Sun Ding-Yu Tan Tian-Yu Xin Yu-Guo Chen Xiao-Dong Zhao Xue-Zhong Yu Jun Xu Emergency Medical Doctor Branch of the Chinese Medical Doctor Association | 2023 | Military Medical Research2023，10，6： | 3 |
| 18 | Brain-derived neurotrophic factor protects neurons from GdCl_3-induced impairment in neuron-astrocyte co-cultures显示文摘Gadolinium (Gd3+) complexes are important contrast agents in medical magnetic resonance imaging (MRI) and of great potential value in brain research. In order to better understand the mechanisms of the action of Gd3+ on neurons in the complex central nervous system (CNS), the neurotoxic actions of GdCl3 have been investigated in both neuron monoculture and astrocyte-neuron co-culture systems. Measurements of lactate dehydrogenase release showed that GdCl3 causes significant cell death of monocultured neurons as a result of reactive oxygen species (ROS) generation and down-regulation of brain-derived neurotrophic factor (BDNF). However, GdCl3 does not affect the viability and BDNF expression of astrocytes. Both co-culturing of neurons with astrocytes and addition of BDNF ameliorated GdCl3-induced neurotoxicity by decreasing ROS generation and facilitating recovery of BDNF levels. The results obtained suggest that astrocytes in the CNS may protect neurons from GdCl3-induced impairment through secreting BDNF and thus up-regulating BDNF expression and interfering with Gd3+-induced cell signaling in neurons. A possible molecular mechanism is suggested which should be helpful in understand- ing the neurotoxic actions of gadolinium probes . | XIA Qing, FENG XuDong, YUAN Lan, WANG Kui & YANG XiaoDa State Key Laboratory of Natural and Biomimetic Drugs and Department of Chemical Biology School of Pharmaceutical Sciences, Peking University, Beijing 100191, China | 2010 | Science China Chemistry2010，53，10： | 3 |
| 19 | Identification of the miniature pig inbred line by skin allograft显示文摘Skin grafting has been used as one of the most reliable tests to determine the genetic stability of laboratory animal such as mice and rats inbred line, but no identification of swine inbred lines by skin grafting has been reported. At present, Wuzhishan miniature pig(WZSP) inbred line has acquired the F24 individuals in China. In order to verify whether WZSP inbred line had been cultivated successfully, allogeneic skin grafts and related research were performed on F20 individuals of WZSP inbreeding population, compared with a control group of autologous transplantation. We observed the transplant recipients' wounds, detected peripheral blood-related indicators interleukin-2, 4 and 10, CD4+ and CD8+ lymphocytes, and conducted hematoxylin-eosin(HE) and Masson's staining of skin to judge whether the immune rejection reactions occurred within 28 days after transplantation. Chr. 7 genomic heterozygosity of 48 WZSP individuals from F20 to F22 was analyzed by high-density single nucleotide polymorphism(SNP) chips(60 000 SNPs). The result showed that there were no significant differences in graft skin, the plasma interleukin-2, 4, 10, CD4+ and CD8+, HE and Masson's staining results between the allograft and autograft groups, and no immune rejection occurred on the allograft group. We found that 11 genes in Chr. 7 of major histocompatibility complex(MHC) I and MHC II were homozygous which confirmed that immune antibody of the allograft and autograft groups were highly identical and also provided a theoretical basis to no immune rejection occurred on the allograft in the inbred WZSP. The result proved that the WZSP inbred line had been cultivated successfully for the first time in the world. The test methods also provide a scientific basis for the identification of swine and mammal inbred lines. | MU Yu-lian LIU Lan FENG Shu-tang WU Tian-wen LI Kui LI Jun-you HE Wei GAO Qian ZHOU Wen-fang WEI Jing-liang TANG Fang YANG Shu-lin WU Zhi-gu XIA Ying SUN Tong-zhu | 2015 | Journal of Integrative Agriculture2015，14，7： | 2 |
| 20 | Mechanistic Studies of Atmospheric Corrosion Behavior of Al and Al-based Alloys in a Tropical Marine Environment显示文摘Atmospheric corrosion behavior of pure Al 1050 A, 5A02 and 6A02 aluminum alloys exposed to a tropical marine environment for 4 years was investigated. Synergetic effect of Cl^-deposition rate and time of wetness resulted in an abnormal increase in weight loss and a significant fluctuation in corrosion rate. Pitting corrosion occurred on the three metals. Pits on 5A02 alloy were easy to initiate and inclined to propagate laterally to form higher corrosion area and shallower corrosion pits, while pits on 6A02 alloy presented the opposite appearances. This was further confirmed by the cyclic polarization experiments. | 崔中雨 GE Feng 李晓刚 ZHU Min XIAO Kui DONG Chaofang WANG Xin | 2017 | Journal of Wuhan University of Technology(Materials Science)2017，32，3： | 2 |
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