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| 1 | One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs显示文摘CRISPR/Cas9 系统是一个有效编辑基因的方法,但是编辑基因的动物的多数显示出 mosaicism,与编辑仅仅在房间的部分发生。这里,我们证明那单个基因或多重基因能被 Cas9 mRNA 和多重邻近的单个指南的 RNA 的 zygotic 注射完全在老鼠和猴子胚胎击倒(spaced 10-200 bp 分开) 指向仅仅一把单个钥匙每基因的 exon。个别地在 Y 染色体上跟随八基因的指向的删除的 F0 老鼠的 Phenotypic 分析在产生猛烈老鼠表明了这条途径的坚韧性。重要地,这条途径在高效率交付完全的基因大美人(Arntl 上的 100% 并且 91% 在 Prrt2 上) 在猴子胚胎。最后,我们能在一个单个步骤产生一只完全的 Prrt2 猛烈猴子,表明在很快建立编辑基因的猴子模型的这条途径的实用性。 | Erwei Zuo Yi-Jun Cai Kui Li Yu Wei Bang-An Wang Yidi Sun Zhen Liu Jiwei Liu Xinde Hu Wei Wei Xiaona Huo Linyu Shi Cheng Tang Dan Liang Yan Wang Yan-Hong Nie Chen-Chen Zhang Xuan Yao Xing Wang Changyang Zhou Wenqin Ying Qifang Wang Ren-Chao Chen Qi Shen Guo-Liang Xu Jinsong Li Qiang Sun Zhi-Qi Xiong Hui Yang | 2017 | Cell Research2017，27，7： | 21 |
| 2 | Flame retardant polyamide 6 by in situ polymerization of ε-caprolactam in the presence of melamine derivatives显示文摘An improved method for preparing melamine cyanurate (MCA) based flame retardant polyamide 6 (FRPA6) materials has been proposed. This processing method, i.e., improved in situ polymerization, was used to synthesize flame retardant PA6. In situ formed MCA nanoparticles were supposed to be linked to PA6 chains in the e-caprolactam hydrolytic polymerization system to obtain star- type polymers for the first time. Through TEM photographs, it can be found that the in situ formed MCA nanoparticles with diametric size of less than 50 nm, are nanoscaled, highly uniformly dispersed in the PA6 matrix. Synthesized ?ame retardant PA6 have good fire performance which can achieve UL-94 V-0 rating at 1.6 mm thickness with the presence of 7.34 wt.% MCA in the matrix. | Zhi Yong Wu Wei Xu Jin Kui Xia Yao Chi Liu Qian Xin Wu Wei Jian Xu | 2008 | Chinese Chemical Letters2008，19，2： | 15 |
| 3 | Application of a catch-based method for stock assessment of three important fisheries in the East China Sea显示文摘Most fisheries in China do not have maximum sustainable yield(MSY) estimates due to limited and poor data.Therefore, finding a common method to estimate MSY or total allowable catch(TAC) for fishery management is necessary. MSYs of three important fisheries in the East China Sea were evaluated through a catch-based model.Estimates for intrinsic rate of increase(r) and five levels of process error were considered. Results showed hairtail Trichiurus japonicas(Temminck and Schlegel) and small yellow croaker Larimichthys polyactis(Bleeker) fisheries experienced overfishing from the mid-1990 s to the early 2000 s, and the suggested TACs were 55.8×10~4 t and9.06×10~4 t, respectively. Decades of overfishing in wintering and spawning grounds of large yellow croaker Larimichthys crocea(Richardson) caused the fishery's collapse in the 1980 s, and it has not recovered until today.The Catch–MSY model generated similar estimated MSYs with other methods and may be a useful choice for the assessment of regional stocks in China. | ZHANG Kui ZHANG Jun XU Youwei SUN Mingshuai CHEN Zuozhi YUAN Meng | 2018 | Acta Oceanologica Sinica2018，37，2： | 14 |
| 4 | Deposition of FeCoNiCrMn high entropy alloy(HEA) coating via cold spraying显示文摘High entropy alloys(HEAs) are of great interest in the community of materials science and engineering due to their unique phase structure. They are constructed with five or more principal alloying elements in equimolar or near-equimolar ratio. Therefore, HEAs can derive their performance from multiple principal elements rather than a single element. In this work, solid-state cold spraying(CS) was applied for the first time to produce FeCoNiCrMn HEA coating. The experimental results confirm that CS can be used to produce a thick HEA coating with low porosity. As a low-temperature deposition process, CS completely retained the HEA phase structure in the coating without any phase transformation. The characterization also reveals that the grains in the CSed HEA coating had experienced significant refinement as compared to those in the as-received HEA powder due the occurrence of dynamic recrystallization at the highly deformed interparticle region. Due to the increased dislocation density and grain boundaries,CSed HEA coating was much harder than the as-received powder. The tribological study shows that the CSed FeCoNiCrMn HEA coating resulted in lower wear rate than laser cladded HEA coatings. | Shuo Yin Wenya Li Bo Song Xingchen Yan Min Kuang Yaxin Xu Kui Wen Rocco Lupoi | 2019 | Journal of Materials Science & Technology2019，35，6： | 13 |
| 5 | Serum Uric Acid is Associated with the Predicted Risk of Prevalent Cardiovascular Disease in a Community-dwelling Population without Diabetes显示文摘Objective To examine the association between serum uric acid levels and cardiovascular disease risk among individuals without diabetes. Methods We investigated the association between serum uric acid levels and the risk of prevalent cardiometabolic diseases, 10-year Framingham risk for coronary heart disease, and 10-year risk for atherosclerotic cardiovascular diseases(ASCVD) among 8,252 participants aged ≥ 40 years without diabetes from Jiading district, Shanghai, China. Results Body mass index, waist circumference, blood glucose, glycated hemoglobin, blood pressure, and serum lipids increased progressively across the sex-specific quartiles of uric acid(all P trend < 0.05). Compared with individuals in the lowest quartile, those in the higher quartiles had a significantly higher prevalence of obesity, hypertension, and dyslipidemia(all P trend < 0.05). A fully adjusted logistic regression analysis revealed that individuals in the highest quartile had an increased risk of predicted cardiovascular disease compared with those in the lowest quartile of uric acid. The multivariate adjusted odds ratios(ORs) [95% confidence intervals(CIs)] for the highest quartiles for high Framingham risk were 3.00(2.00-4.50) in men and 2.95(1.08-8.43) in women. The multivariate adjusted ORs(95% CIs) for the highest quartile for high ASCVD risk were 1.93(1.17-3.17) in men and 4.53(2.57-7.98) in women. Conclusion Serum uric acid level is associated with an increased risk of prevalent obesity, hypertension, dyslipidemia, 10-year Framingham risk for coronary heart disease, and 10-year risk for ASCVD among Chinese adults without diabetes. | CHENG Di DU Rui WU Xue Yan LIN Lin PENG Kui MA Li Na XU Yu XU Min CHEN Yu Hong BI Yu Fang WANG Wei Qing DAI Meng LU Jie Li | 2018 | Biomedical and Environmental Sciences2018，31，2： | 13 |
| 6 | A new understanding of the effect of Cr on the corrosion resistance evolution of weathering steel based on big data technology显示文摘In this work,we studied the effect of Cr element on the corrosion resistance evolution of weathering steel based on corrosion big data technology.It suggested that corrosion big data technology is suitable for evaluation of the effect of microalloying Cr element on the corrosion evolution behavior of weathering steel.New understandings prove that the effect of Cr on the corrosion process is dynamic rather than static,the processes is affected by both of the environmental factors and the electrochemical or chemical reactions in the rust layer.Besides,Cr element has both beneficial effect and detrimental effect on the corrosion resistance of weathering steel.The beneficial effect is that the general corrosion resistance of Cr-additional steel is better than that of Cr-free steel,while the detrimental effect is that localized corrosion is intensified as the increase of Cr content in the Cr-additional steel. | Xiaojia Yang Ying Yang Meihui Sun Jinghuan Jia Xuequn Cheng Zibo Pei Qing Li Di Xu Kui Xiao Xiaogang Li | 2022 | Journal of Materials Science & Technology2022，，9： | 12 |
| 7 | Structural evolution of non dendritic AlSi7Mg alloy during reheating显示文摘The structural evolution of non dendritic AlSi7Mg alloy during reheating in resistance furnace was studied. The alloy ingots were produced by electromagnetic stirring during solidification. It was found that, the Si phase in eutectic dissolves in a way of diffusion toward α phase, its appearance changes from flake to dot like, and tends to be fine and spheroidal with increasing reheating temperature. The thinner the flake, the lower the temperature for the occurrence of this process, and the higher the transforming speed. The eutectic melts partially when Si phase dissolves to some extent, and the morphology and size of primary α phase begin to change. The dendrite and rosette α phases tends to sphericize. The size of the former becomes larger, while the size of the latter reduces to be 1/2~1/4 of the original size. The spheroidal primary α phase has a tendency of grain growth. | Zhang Kui(张奎) Zhang Yongzhong(张永忠) Liu Guojun(刘国均) Xu Jun(徐骏) Zhang Jingxin(张景新) Fan Jianzhong(樊建中) Shi Likai(石力开) | 1999 | 中国有色金属学会会刊:英文版1999，9，3： | 10 |
| 8 | A transgene-free method for rapid and efficient generation of precisely edited pigs without monoclonal selection显示文摘Gene-edited pigs for agricultural and biomedical applications are typically generated using somatic cell nuclear transfer(SCNT).However, SCNT requires the use of monoclonal cells as donors, and the time-consuming and laborious monoclonal selection process limits the production of large populations of gene-edited animals. Here, we developed a rapid and efficient method named RE-DSRNP(reporter RNA enriched dual-sg RNA/CRISPR-Cas9 ribonucleoproteins) for generating gene-edited donor cells. RE-DSRNP takes advantage of the precise and efficient editing features of dual-sg RNA and the high editing efficiency, low off-target effects, transgene-free nature, and low cytotoxic characteristics of reporter RNA enriched RNPs(CRISPR-Cas9ribonucleoproteins), thus eliminating the need for the selection of monoclonal cells and thereby greatly reducing the generation time of donor cells from 3–4 weeks to 1 week, while also reducing the extent of apoptosis and chromosomal aneuploidy of donor cells. We applied RE-DSRNP to produce cloned pigs bearing a deletion edit of the wild-type p53-induced phosphatase 1(WIP1)gene: among 32 weaned cloned pigs, 31(97%) carried WIP1 edits, and 15(47%) were homozygous for the designed fragment deletion, and no off-target event was detected. The WIP1 knockout(KO) pigs exhibited male reproductive disorders, illustrating the utility of RE-DSRNP for rapidly generating precisely edited animals for functional genomics and disease research. REDSRNP's strong editing performance in a large animal and its marked reduction in the required time for producing SCNT donor cells support its application prospects for rapidly generating populations of transgene-free cloned animals. | Kui Xu Xiuling Zhang Zhiguo Liu Jinxue Ruan Changjiang Xu Jingjing Che Ziyao Fan Yulian Mu Kui Li | 2022 | Science China(Life Sciences)2022，65，8： | 9 |
| 9 | CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells显示文摘In face of the everlasting battle toward COVID-19 and the rapid evolution of SARS-CoV-2,no specific and effective drugs for treating this disease have been reported until today.Angiotensin-converting enzyme 2(ACE2),a receptor of SARS-CoV-2,mediates the virus infection by binding to spike protein.Although ACE2 is expressed in the lung,kidney,and intestine,its expressing levels are rather low,especially in the lung.Considering the great infectivity of COVID-19,we speculate that SARS-CoV-2 may depend on other routes to facilitate its infection.Here,we first discover an interaction between host cell receptor CD147 and SARS-CoV-2 spike protein.The loss of CD147 or blocking CD147 in Vero E6 and BEAS-2B cell lines by anti-CD147 antibody,Meplazumab,inhibits SARSCoV-2 amplification.Expression of human CD147 allows virus entry into non-susceptible BHK-21 cells,which can be neutralized by CD147 extracellular fragment.Viral loads are detectable in the lungs of human CD147(hCD147)mice infected with SARS-CoV-2,but not in those of virus-infected wild type mice.Interestingly,virions are observed in lymphocytes of lung tissue from a COVID-19 patient.Human T cells with a property of ACE2 natural deficiency can be infected with SARS-CoV-2 pseudovirus in a dosedependent manner,which is specifically inhibited by Meplazumab.Furthermore,CD147 mediates virus entering host cells by endocytosis.Together,our study reveals a novel virus entry route,CD147-spike protein,which provides an important target for developing specific and effective drug against COVID-19. | Ke Wang Wei Chen Zheng Zhang Yongqiang Deng Jian-Qi Lian Peng Du Ding Wei Yang Zhang Xiu-Xuan Sun Li Gong Xu Yang Lei He Lei Zhang Zhiwei Yang Jie-Jie Geng Ruo Chen Hai Zhang Bin Wang Yu-Meng Zhu Gang Nan Jian-Li Jiang Ling Li Jiao Wu Peng Lin Wan Huang Liangzhi Xie Zhao-Hui Zheng Kui Zhang Jin-Lin Miao Hong-Yong Cui Min Huang Jun Zhang Ling Fu Xiang-Min Yang Zhongpeng Zhao Shihui Sun Hongjing Gu Zhe Wang Chun-Fu Wang Yacheng Lu Ying-Ying Liu Qing-Yi Wang Huijie Bian Ping Zhu Zhi-Nan Chen | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 9 |
| 10 | Area and volume ratios for prediction of visual outcome in idiopathic macular hole显示文摘AIM:To predict the visual outcome in patients undergoing macular hole surgery by two novel three-dimensional morphological parameters on optical coherence tomography(OCT): area ratio factor(ARF) and volume ratio factor(VRF).METHODS: A clinical case series was conducted,including 54 eyes of 54 patients with an idiopathic macular hole(IMH). Each patient had an OCT examination before and after surgery. Morphological parameters of the macular hole,such as minimum diameter,base diameter,and height were measured. Then,the macular hole index(MHI),tractional hole index(THI),and hole form factor(HFF) were calculated. Meanwhile,novel postoperative macular hole(MH) factors,ARF and VRF were calculated by three-dimensional morphology. Bivariate correlations were performed to acquire asymptotic significance values between the steady best corrected visual acuity(BCVA) after surgery and 2D/3D arguments of MH by the Pearson method with two-tailed test. All significant factors were analyzed by the receiver operating characteristic(ROC) curve analysis of SPSS software which were responsible for vision recovery. ROC curves analyses were performed to further discuss the different parameters on the prediction of visual outcome.RESULTS: The mean and standard deviation values of patients' age,symptoms duration,and follow-up time were 64.8±8.9y(range: 28-81),18.6±11.5d(range: 2-60),and 11.4±0.4mo(range: 6-24),respectively. Steady-postBCVA analyzed with bivariate correlations was found to be significantly correlated with base diameter(r=0.521,P<0.001),minimum diameter(r=0.514,P<0.001),MHI(r=-0.531,P<0.001),THI(r=-0.386,P=0.004),HFF(r=-0.508,P<0.001),and ARF(r=-0.532,P<0.001). Other characteristic parameters such as age,duration of surgery,height,diameter hole index,and VRF were not statistically significant with steady-post-BCVA. According to area under the curve(AUC) values,values of ARF,MHI,HFF,minimum diameter,THI,and base diameter are 0.806,0.772,0.750,0.705,0.690,and 0.686,respectively. However,Steady-post-BCVA analysis with bivariate correlations for VRF was no statistical significance. Results of ROC curve analysis indicated that the MHI value,HFF,and ARF was greater than 0.427,1.027 and 1.558 respectively which could correlate with better visual acuity.CONCLUSION: Compared with MHI and HFF,ARF could effectively express three-dimensional characteristics of macular hole and achieve better sensitivity and specificity. Thus,ARF could be the most effective parameter to predict the visual outcome in macular hole surgery. | Xing-Yun Geng Hui-Qun Wu Jie-Hui Jiang Kui Jiang Jun Zhu Yi Xu Jian-Cheng Dong Zhuang-Zhi Yan | 2017 | International Journal of Ophthalmology(English edition)2017，10，8： | 8 |
| 11 | Variations in paleoproductivity and the environmental implications over the past six decades in the Changjiang Estuary显示文摘Total organic carbon and organic carbon stable isotope, biogenic silica, chlorin were measured from a high resolution sediment core to indicate the variation in paleoproductivity and the environmental implications over the past decades (1942 to 1997) in the Changjiang Estuary. Based on these biomarkers, the shift in the phytoplankton community structure in the past decades was discussed in response to the long-term variations in nutrient concentrations and nutrient ratios. The results showed that the δ 13 C values varied from –26.15‰ to –19.5‰, suggesting the combined organic carbon sources of riverine and marine organisms. Based on the biogenic proxies, paleoproductivity changes were categorized into three stages: low production before 1950; an increase in production with the dominance of diatoms during 1950 to 1980, consistent with increasing of nutrient concentrations; a decrease in production after 1980 when the diatom production decreased while the production of non-silicious phytoplankton increased due to high nutrient inputs, and high N/P and P/Si ratios in the Changjiang Estuary. The sedimentation records also indicated that the riverine organic carbon increased since 1980. | JIN Haiyan CHEN Jianfang WENG Huanxin LI Hongliang ZHANG Weiyan XU Jie BAI Youcheng WANG Kui | 2010 | Acta Oceanologica Sinica2010，29，3： | 8 |
| 12 | Review of CHINARE chemical oceanographic research in the Southern Ocean during 1984–2016显示文摘Between 1984 and 2016, China executed 33 Antarctic cruises with the icebreaker R/V Xuelong, which have provided opportunities for Chinese scientists to investigate the status and changes of the Southern Ocean. Research in chemical oceanography constitutes one of the primary missions of the Chinese National Antarctic Research Expedition(CHINARE). This paper reviews nearly 30 years of Chinese Antarctic expeditions, focusing on the major progress achieved in chemical oceanographic research. Specifically, the sea-surface distributions and air–sea fluxes of CO_2 and N_2O are considered, and the transport, flux, and budget of organic matter are investigated based on isotopes in the Southern Ocean, especially in Prydz Bay. In addition, the nutrient distribution and deep-water particle export in Prydz Bay and the study of aerosol heavy metal characteristics are considered. Finally, the prospects for future Chinese Antarctic chemical oceanographic research are outlined. | CHEN Liqi CHEN Min ZHAN Liyan QI Di GAO Zhongyong WANG Jianjun XU Suqing CHEN Kui ZENG Jian ZHANG Run | 2017 | Advances in Polar Science2017，28，2： | 6 |
| 13 | Relationship between reduced nicotinamide adenine dinucleotide phosphate oxidase subunit p22phox gene polymorphism and obstructive sleep apnea-hypopnea syndrome in the Chinese Han population显示文摘背景反应的氧种类(ROS ) 的增加的生产被认为在妨碍的睡觉呼吸暂停呼吸过慢过浅症候群(OSAHS ) 的致病起一个主要作用。减少的菸碱腺嘌 dinucleotide 磷酸盐(NADPH ) oxidase 建筑群是 ROS 的重要来源。p22phox 子单元与在潜在的 heme 绑定地点为酷氨酸改变 histidine-72 的 C242T 变体是多态的。这研究试图调查在 NADPH oxidase 子单元 p22phox 基因多型性和 OSAHS 之间的关系。 p22phox 多型性的遗传型被聚合酶链反应限制决定的方法碎裂在在中国的南部的区域的汉人口的 176 个无关的题目的长度多型性( PCR-RFLP )试金(包括 107 个 OSAHS 题目和 69 non-OSAHS 使遭到),当 superoxide dismutase (草皮)的血浆集中在外部血 mononuclear 在二个组,和 p22phox mRNA 表示被检测时,房间( PBMC )与反向的抄写聚合酶链 re 被决定与 non-OSAHS 控制相比组织((85.31 牥吗?? | LIU Hui-guo LIU Kui ZHOU Yan-ning XU Yong-jian | 2009 | Chinese Medical Journal2009，，12： | 6 |
| 14 | CD147 antibody specifically and effectively inhibits infection and cytokine storm of SARS-CoV-2 and its variants delta,alpha,beta,and gamma显示文摘SARS-CoV-2 mutations contribute to increased viral transmissibility and immune escape,compromising the effectiveness of existing vaccines and neutralizing antibodies.An in-depth investigation on COVID-19 pathogenesis is urgently needed to develop a strategy against SARS-CoV-2 variants.Here,we identified CD147 as a universal receptor for SARS-CoV-2 and its variants.Meanwhile,Meplazeumab,a humanized anti-CD147 antibody,could block cellular entry of SARS-CoV-2 and its variants-alpha,beta,gamma,and delta,with inhibition rates of 68.7,75.7,52.1,52.1,and 62.3%at 60μg/ml,respectively.Furthermore,humanized CD147 transgenic mice were susceptible to SARS-CoV-2 and its two variants,alpha and beta.When infected,these mice developed exudative alveolar pneumonia,featured by immune responses involving alveoli-infiltrated macrophages,neutrophils,and lymphocytes and activation of IL-17 signaling pathway.Mechanistically,we proposed that severe COVID-19-related cytokine storm is induced by a'spike protein-CD147-CyPA signaling axis':Infection of SARS-CoV-2 through CD147 initiated the JAK-STAT pathway,which further induced expression of cyclophilin A(CyPA);CyPA reciprocally bound to CD147 and triggered MAPK pathway.Consequently,the MAPK pathway regulated the expression of cytokines and chemokines,which promoted the development of cytokine storm.Importantly,Meplazumab could effectively inhibit viral entry and inflammation caused by SARS-CoV-2 and its variants.Therefore,our findings provided a new perspective for severe COVID-19-related pathogenesis.Furthermore,the validated universal receptor for SARS-CoV-2 and its variants can be targeted for COVID-19 treatment. | Jiejie Geng Liang Chen Yufeng Yuan Ke Wang Youchun Wang Chuan Qin Guizhen Wu Ruo Chen Zheng Zhang Ding Wei Peng Du Jun Zhang Peng Lin Kui Zhang Yongqiang Deng Ke Xu Jiangning Liu Xiuxuan Sun Ting Guo Xu Yang Jiao Wu Jianli Jiang Ling Li Kun Zhang Zhe Wang Jing Zhang Qingguo Yan Hua Zhu Zhaohui Zheng Jinlin Miao Xianghui Fu Fengfan Yang Xiaochun Chen Hao Tang Yang Zhang Ying Shi Yumeng Zhu Zhuo Pei Fei Huo Xue Liang Yatao Wang Qingyi Wang Wen Xie Yirong Li Mingyan Shi Huijie Bian Ping Zhu Zhi-Nan Chen | 2021 | Signal Transduction and Targeted Therapy2021，6，10： | 6 |
| 15 | Magnetostratigraphy and ^(230)Th dating of a drill core from the southeastern Qaidam Basin:Salt lake evolution and tectonic implications显示文摘The Qarhan Salt Lake area is the Quaternary depocenter of the Qaidam Basin, and carries thick lacustrine sediments, as well as rich potassium and magnesium salt deposits. The abundant resources and thick sediments in this lake provide an ideal place for the study of biogas formation and preservation, salt lake evolution, and the uplift of the Tibetan Plateau. In this study, we attempt to construct a paleomagnetic and ^(230)Th age model and to obtain information on tectonic activity and salt lake evolution through detailed studies on a 1300-m-long drill core(15DZK01) from the northwestern margin of the Qarhan Salt Lake area(Dongling Lake). Based on gypsum ^(230)Th dating, the age of the uppermost clastic deposit was calculated to be around 0.052 Ma. The polarity sequence consist of 13 pairs of normal and reversed zones,which can be correlated with subchrons C2r.1r-C1n of the geomagnetic polarity timescale(GPTS 2012)(from ~2.070 Ma to ~0.052 Ma). Sedimentary characteristics indicate that Dongling Lake witnessed freshwater environment between ~ 2.070 Ma and 1.546 Ma. During this period, the sedimentary record reflects primarily lakeshore, shallow-water and swamp environments, representing favourable conditions for the formation of hydrocarbon source rocks. Between 1.546 Ma and ~ 0.052 Ma, the Dongling Lake was in sulphate deposition stage, which contrasts with the central Qarhan Salt Lake area, where this stage did not occur in the meantime. During this stage, Dongling Lake was in a shallow saltwater lake environment, but several periods of reduced salinity occurred during this stage. During the late Pleistocene at ~0.052 Ma, the Dongling Lake experienced uplift due to tectonic activity, and saltwater migrated through the Sanhu Fault to the central Qarhan Salt Lake area, resulting in the absence of halite deposition stage. The residual saline water was concentrated into magnesium-rich brine due to the lack of freshwater, and few potassium salt deposits occur in the Dongling Lake area. | An-Dong Chen Mian-Ping Zheng Hai-Tao Yao Kui Su Jian-Ming Xu | 2018 | Geoscience Frontiers2018，9，3： | 6 |
| 16 | Predicting dynamic cellular protein–RNA interactions by deep learning using in vivo RNA structures显示文摘Interactions with RNA-binding proteins(RBPs)are integral to RNA function and cellular regulation,and dynamically reflect specific cellular conditions.However,presently available tools for predicting RBP–RNA interactions employ RNA sequence and/or predicted RNA structures,and therefore do not capture their condition-dependent nature.Here,after profiling transcriptome-wide in vivo RNA secondary structures in seven cell types,we developed PrismNet,a deep learning tool that integrates experimental in vivo RNA structure data and RBP binding data for matched cells to accurately predict dynamic RBP binding in various cellular conditions.PrismNet results for 168 RBPs support its utility for both understanding CLIP-seq results and largely extending such interaction data to accurately analyze additional cell types.Further,PrismNet employs an“attention”strategy to computationally identify exact RBP-binding nucleotides,and we discovered enrichment among dynamic RBP-binding sites for structure-changing variants(riboSNitches),which can link genetic diseases with dysregulated RBP bindings.Our rich profiling data and deep learning-based prediction tool provide access to a previously inaccessible layer of cell-type-specific RBP–RNA interactions,with clear utility for understanding and treating human diseases. | Lei Sun Kui Xu Wenze Huang Yucheng T.Yang Pan Li Lei Tang Tuanlin Xiong Qiangfeng Cliff Zhang | 2021 | Cell Research2021，31，5： | 6 |
| 17 | Epidemiological Characteristics of Echinococcosis in Non-Endemic PLADs—China,2017–2020显示文摘Summary What is already known about this topic?Echinococcosis,also known as hydatid disease,is a zoonotic parasitic disease caused by the larvae of Echinococcus granulosus.Western China has one of the most severe epidemics worldwide.Echinococcosis is endemic in 370 counties of 9 provincial-level administrative divisions(PLADs)including Inner Mongolia,Sichuan,Yunnan,Tibet,Shaanxi,Gansu,Qinghai,Ningxia,Xinjiang and Xinjiang Production and Construction Corps(XPCC). | Yan Kui Baixue Liu Xu Wang Cuizhao Xue Jingbo Xue Yalan Zhang Benguang Zhang Yang Dai Shuai Han Weiping Wu | 2021 | China CDC weekly2021，3，51： | 5 |
| 18 | PbGA2ox8 induces vascular-related anthocyanin accumulation and contributes to red stripe formation on pear fruit显示文摘Fruit with stripes,which are generally longitudinal,can occur naturally,but the bioprocesses underlying this phenomenon are unclear.Previously,we observed an atypical anthocyanin distribution that caused red-striped fruit on the spontaneous pear bud sport“Red Zaosu”(Pyrus bretschneideri Rehd.).In this study,comparative transcriptome analysis of the sport and wild-type“Zaosu”revealed that this atypical anthocyanin accumulation was tightly correlated with abnormal overexpression of the gene-encoding gibberellin(GA)2-beta-dioxygenase 8,PbGA2ox8.Consistently,decreased methylation was also observed in the promoter region of PbGA2ox8 from“Red Zaosu”compared with“Zaosu”.Moreover,the GA levels in“Red Zaosu”seedlings were lower than those in“Zaosu”seedlings,and the application of exogenous GA4 reduced abnormal anthocyanin accumulation in“Red Zaosu”.Transient overexpression of PbGA2ox8 reduced the GA4 level and caused anthocyanin accumulation in pear fruit skin.Moreover,the presence of red stripes indicated anthocyanin accumulation in the hypanthial epidermal layer near vascular branches(VBs)in“Red Zaosu”.Transient overexpression of PbGA2ox8 resulting from vacuum infiltration induced anthocyanin accumulation preferentially in calcium-enriched areas near the vascular bundles in pear leaves.We propose a fruit-striping mechanism,in which the abnormal overexpression of PbGA2ox8 in“Red Zaosu”induces the formation of a longitudinal array of anthocyanin stripes near vascular bundles in fruit. | Rui Zhai Zhigang Wang Chengquan Yang Kui Lin-Wang Richard Espley Jianlong Liu Xieyu Li Zhongying Wu Pengmin Li Qingmei Guan Fengwang Ma Lingfei Xu | 2019 | Horticulture Research2019，6，1： | 5 |
| 19 | Long-term,multidomain analyses to identify the breed and allelic effects in MSTN-edited pigs to overcome lameness and sustainably improve nutritional meat production显示文摘Beef and mutton production has been aided by breeding to integrate allelic diversity for myostatin(MSTN),but a lack of diversity in the MSTN germplasm has limited similar advances in pig farming.Moreover,insurmountable challenges with congenital lameness and a dearth of data about the impacts of feed conversion,reproduction,and meat quality in MSTN-edited pigs have also currently blocked progress.Here,in a largest-to-date evaluation of multiple MSTN-edited pig populations,we demonstrated a practical alternative edit-site-based solution that overcomes the major production obstacle of hindlimb weakness.We also provide long-term and multidomain datasets for multiple breeds that illustrate how MSTN-editing can sustainably increase the yields of breed-specific lean meat and the levels of desirable lipids without deleteriously affecting feed-conversion rates or litter size.Apart from establishing a new benchmark for the data scale and quality of genome-edited animal production,our study specifically illustrates how gene-editing site selection profoundly impacts the phenotypic outcomes in diverse genetic back-grounds. | Ziyao Fan Zhiguo Liu Kui Xu Tianwen Wu Jinxue Ruan Xinmin Zheng Shideng Bao Yulian Mu Tad Sonstegard Kui Li | 2022 | Science China(Life Sciences)2022，65，2： | 5 |
| 20 | Designing a Phthalonitrile/Benzoxazine Blend for the Advanced GFRP Composite Materials显示文摘High performance resin must be used in the high performance glass fiber-reinforced plastic(GFRP) composites, but it is sometimes difficult to balance the processabilities and the final properties in the design of advanced thermoset GFRP composites. In this study, a phthalonitrile/benzoxazine(PPN/BZ) blend with excellent processability has been designed and applied in the GFRP composite materials. PPN/BZ blend with good solubility, low melt viscosity, appropriate gel condition and low-temperature curing behavior could enable their GFRP composite preparation with the prepreg-laminate method under a relatively mild condition. The resulted PPN/BZ GFRP composites exhibit excellent mechanical properties with flexural strength over 700 MPa and flexural modulus more than 19 GPa. Fracture surface morphologies of the PPN/BZ GFRP composites show that the interfacial adhesion between resin and GF is improved. The temperatures at weight loss 5%(T_(5%)) and char residue at 800 °C of all PPN/BZ GFRP composites are over 435 °C and 65% respectively. PPN/BZ GFRP composites with high performance characteristics may find applications under some critical circumstances with requirements of high mechanical properties and high service temperatures. | Xu-Lin Yang Kui Li Ming-Zhen Xu Xiao-Bo Liu | 2018 | Chinese Journal of Polymer Science2018，36，1： | 5 |
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