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| 1 | Generation of iPSCs from mouse fibroblasts with a single gene, Oct4, and small molecules显示文摘由病毒的 transduction 的四抄写因素 Oct4, Klf4, Sox2 和 c-Myc 的介绍能导致体的房间的 reprogramming 进导致的 pluripotent 干细胞(iPSCs ) ,但是 iPSCs 的使用被病毒的交货系统的使用妨碍。导致化学药品的 reprogramming 把一条新奇途径提供给没有任何病毒的基于向量的基因修正,产生 iPSCs。尽管,以前的报告证明几个小分子能代替一些 reprogramming 因素至少二个抄写因素, Oct4 和 Klf4,仍然被要求从老鼠产生 iPSCs 胚胎的成纤维细胞。这里,我们识别特定的化学联合,它是足够的面对一个单个抄写因素从胚胎的老鼠和成年成纤维细胞允许 reprogramming, Oct4 在 20 天以内,代替 Sox2, Klf4 和 c-Myc。用这个处理产生的 iPSCs 类似于胚胎的干细胞以全球基因表示介绍的老鼠,在 vitro 并且在 vivo 的 epigenetic 地位和 pluripotency。我们也发现那 8 天 Oct4 正式就职是足够的面对小分子启用导致 Oct4 的 reprogramming,它建议 reprogramming 在开始的 8 天以内被开始并且独立于连续外长的 Oct4 表示。没有基因修正,这些发现将帮助 iPSCs 的未来产生,以及阐明位于 reprogramming 过程下面的分子的机制。 | Yanqin Li Qiang Zhang Xiaolei Yin Weifeng Yang Yuanyuan Du Pingping Hou Jian Ge Chun Liu Weiqi Zhang Xu Zhang Yetao Wu Honggang Li Kang Liu Chen Wu Zhihua Song Yang Zhao Yan Shi Hongkui Deng | 2011 | Cell Research2011，21，1： | 51 |
| 2 | Variability and reduction of atmospheric pollutants in Beijing and its surrounding area during the Beijing 2008 Olympic Games显示文摘The Beijing-Tianjin-Hebei Atmospheric Environment Monitoring Network was established by the Institute of Atmospheric Physics,Chinese Academy of Sciences.The goals of the network were to monitor and provide warnings of the atmospheric quality in Beijing and its surrounding area during the Beijing 2008 Olympic Games.The results showed that the atmospheric complex pollution exhibited high concentrations of ozone and fine particles and oxidation in summer,with a ubiquitous regional source.The regional mean concentrations of SO2,PM2.5,NO2,and O3_8h max(the maximum daily 8 h mean) and Ox were 22±11,90±40,25±5,136±35 and 112±21 μg/m3 in summer,respectively.During the Olympic Games,the mean concentration of SO2,PM2.5,NO2,O3_8h max,and Ox were 12.5±4,56±28,23±4,114±29,95±17 μg/m3 in the region,respectively,and fell by 51.0%,43.7%,13%,20.2%,and 18.9%,respectively,compared to the prophase mean before the Olympic Games.The concentration of atmospheric pollutants declined significantly and achieved the 'Green Olympics' control goal of air quality.After the Olympic Games,SO2,PM2.5 and NOx increased significantly as the temporary atmospheric pollution control measures were terminated. | XIN JinYuan WANG YueSi TANG GuiQian WANG LiLi SUN Yang WANG YingHong HU Bo SONG Tao JI DongSheng WANG WeiFeng LI Liang LIU GuangRen | 2010 | Chinese Science Bulletin2010，55，18： | 30 |
| 3 | Origin and molecular characterization of the human-infecting H6N1 infl uenza virus in Taiwan显示文摘In June 2013,the fi rst human H6N1 infl uenza virus infec-tion was confirmed in Taiwan.However,the origin and molecular characterization of this virus,A/Taiwan/2/2013(H6N1),have not been well studied thus far.In the present report,we performed phylogenetic and coalescent analy-ses of this virus and compared its molecular profi le/char-acteristics with other closely related strains.Molecular characterization of H6N1 revealed that it is a typical avian infl uenza virus of low pathogenicity,which might not rep-licate and propagate well in the upper airway in mammals.Phylogenetic analysis revealed that the virus clusters with A/chicken/Taiwan/A2837/2013(H6N1)in seven genes,except PB1.For the PB1 gene,A/Taiwan/2/2013 was clus-tered with a different H6N1 lineage from A/chicken/Taiwan/A2837/2013.Although a previous study demonstrated that the PB2,PA,and M genes of A/Taiwan/2/2013 might be derived from the H5N2 viruses,coalescent analyses revealed that these H5N2 viruses were derived from more recent strains than that of the ancestor of A/Taiwan/2/2013.Therefore,we propose that A/Taiwan/2/2013 is a reassor-tant from different H6N1 lineages circulating in chickens in Taiwan.Furthermore,compared to avian isolates,a sin-gle P186L(H3 numbering)substitution in the hemaggluti-nin H6 of the human isolate might increase the mammali-an receptor binding and,hence,this strain’s pathogenicity in humans.Overall,human infection with this virus seems an accidental event and is unlikely to cause an infl uenza pandemic.However,its co-circulation and potential reas-sortment with other infl uenza subtypes are still worthy of attention. | Weifeng Shi Yi Shi Ying Wu Di Liu George F.Gao | 2013 | Protein & Cell2013，4，11： | 26 |
| 4 | Pluripotent stem cells induced from mouse neural stem cells and small intestinal epithelial cells by small molecule compounds显示文摘最近,我们报导了一条化学途径从老鼠成纤维细胞产生 pluripotent 干细胞。然而,是否化学上导致了 pluripotent 干细胞(CiPSCs ) ,能从要示威的另外的房间类型遗体被导出。用系跟踪,这里,我们首先从成纤维细胞验证 CiPSCs 的产生。下次,我们证明从外胚层的神经干细胞(NSC ) 和从内胚叶的小肠的上皮的房间(IEC ) 能是化学上 reprogrammed 进 pluripotent 干细胞。CiPSCs 源于 NSC 和 IEC 类似于老鼠处于增长率,全球基因表示侧面, epigenetic 地位,自强和区别能力的胚胎的干细胞,和 germline 传播才能。有趣地, pluripotency 基因 Sall4 从不同房间类型,和一样的核心在化学 reprogramming 过程在起始的阶段被表示小分子为 reprogramming 被要求,在从这些多样的房间类型位于化学 reprogramming 下面的分子的机制建议保存。我们的分析也证明这些小分子的使用应该被调整从不同房间类型满足 reprogramming 的要求。一起,这些调查结果证明那条完整的化学 reprogramming 途径能在不同织物起源的房间被使用并且建议化学 reprogramming 是有要延长到更起始的类型的潜力的有希望的策略。 | Junqing Ye Jian Ge Xu Zhang Lin Cheng Zhengyuan Zhang Shan He Yuping Wang Hua Lin Weifeng Yang Junfang Liu Yang Zhao Hongkui Deng | 2016 | Cell Research2016，26，1： | 19 |
| 5 | Biological productivity and carbon cycling in the Arctic Ocean显示文摘Primary production, bacterial production, par-ticulate organic carbon fluxes and organic carbon burial rates were quantified during the summer period of 1999 in the Arctic Ocean via 14C uptake, 3H uptake, 234Th/238U disequilibrium and 210Pbex dating, respectively. The integrated primary production in the water column was as high as 197 mmolC/(m2·d) in the Chukchi shelf and was 3.8 mmolC/(m2·d) in the Canada Basin. These rates are higher than those reported previously. The ratios of bacterial production to primary production in the study region were higher than 0.5, indicating that microbial activity is not depressed but important in cold Arctic waters. 234Th/238U dis-equilibria were evident at the station in the Canada Basin. The presence of significant 234Th deficiency suggested that scavenging and removal processes are also important to bio-geochemical cycles of trace elements in the Arctic Ocean. Particulate organic carbon export flux was estimated to be 1.0 mmolC/(m2·d). Measurements of sediment | CHEN Min HUANG Yipu GUO Laodong CAI Pinghe YANG Weifeng LIU Guangshan QIU Yusheng | 2002 | Chinese Science Bulletin2002，47，12： | 17 |
| 6 | Influence Analysis of Secondary O-ring Seals in Dynamic Behavior of Spiral Groove Gas Face Seals显示文摘The current research on secondary O-ring seals used in mechanical seals has begun to focus on their dynamic properties. However, detailed analysis of the dynamic properties of O-ring seals in spiral groove gas face seals is lacking. In particular a transient study and a difference analysis of steady-state and transient performance are imperative. In this paper, a case study is performed to gauge the effect of secondary O-ring seals on the dynamic behavior(steady-state performance and transient performance) of face seals. A numerical finite element method(FEM) model is developed for the dynamic analysis of spiral groove gas face seals with a flexibly mounted stator in the axial and angular modes. The rotor tilt angle, static stator tilt angle and O-ring damping are selected to investigate the effect of O-ring seals on face seals during stable running operation. The results show that the angular factor can be ignored to save time in the simulation under small damping or undamped conditions. However, large O-ring damping has an enormous effect on the angular phase difference of mated rings, affecting the steady-state performance of face seals and largely increasing the possibility of face contact that reduces the service life of face seals. A pressure drop fluctuation is carried out to analyze the effect of O-ring seals on the transient performance of face seals. The results show that face seals could remain stable without support stiffness and O-ring damping during normal stable operation but may enter a large-leakage state when confronting instantaneous fluctuations. The oscillation-amplitude shortening effect of O-ring damping on the axial mode is much greater than that on the angular modes and O-ring damping prefers to cater for axial motion at the cost of angular motion. This research proposes a detailed dynamic-property study of O-ring seals in spiral groove gas face seals, to assist in the design of face seals. | HU Songtao HUANG Weifeng LIU Xiangfeng WANG Yuming | 2016 | Chinese Journal of Mechanical Engineering2016，29，3： | 14 |
| 7 | MULTI-GRANULARITY EVOLUTION ANALYSIS OF SOFTWARE USING COMPLEX NETWORK THEORY显示文摘软件系统是一种典型类型人造复杂系统。理解他们的进化能导致更好的软件工程惯例。在这份报纸,作者把复杂网络理论用作一个工具分析进化面向对象(OO ) 从一个 multigranularity 观点的软件。首先,网络建模的一个多颗粒度软件被建议从颗粒度的三个层次代表一个多版本软件系统的拓扑的结构。然后,广泛地在复杂网络理论使用的一些参数被使用描绘软件网络。由在连续软件系统跟踪参数值,我们关于软件进化有更好的理解。案例研究在一个开放源代码 OO 工程上被进行, Azureus 当说明我们的途径的一个例子,和位于进化特征下面的一些被揭开。这些结果提供一种不同尺寸给我们软件进化并且也的理解为 OO 软件系统的设计和发展是很有用的。 | Weifeng PAN Bing LI Yutao MA Jing LIU | 2011 | Journal of Systems Science & Complexity2011，24，6： | 13 |
| 8 | The effect of surface roughness on thermal-elasto-hydrodynamic model of contact mechanical seals显示文摘In this paper,the effect of surface roughness on sealing clearance,pressure distribution,friction torque and leakage is studied by the thermal-elasto-hydrodynamic mixed lubrication model.A convergent nominal clearance is formed by the pressure deformation and thermal deformation of the seal faces.This causes more serious wear in the inner side than that of the outer side of the contact area.Mass leakage increases with the growing of the surface roughness.The temperature and thermal deformation on the seal surface increases substantially if the roughness is reduced.The contact mechanical seals have consistent performance when the standard deviation of surface roughness is approximately 0.2μm.In order to validate the theoretical analysis model,a method combining the measurement of three-dimensioned profile and Raman spectrum is proposed. | WEN QingFeng LIU Ying HUANG WeiFeng SUO ShuangFu WANG YuMing | 2013 | Science China(Physics,Mechanics & Astronomy)2013，56，10： | 13 |
| 9 | Earthquake prediction from China's mobile gravity data显示文摘The relation between plate tectonics and earthquake evolution is analyzed systematically on the basis of 1998e2010 absolute and relative gravity data from the Crustal Movement Observation Network of China. Most earthquakes originated in the plate boundary or within the fault zone. Tectonic deformation was most intense and exhibited discontinuity within the tectonically active fault zone because of the differential movement; the stress accumulation produced an abrupt gravity change, which was further enhanced by the earthquake. The gravity data from China's Mainland since 2000 obviously reflected five major earthquakes(Ms > 7), all of which were better reflected than before 2000. Regional gravity anomalies and a gravity gradient change were observed in the area around the epicenter about 2 or 3 years before the earthquake occurred, suggesting that gravity change may be a seismic precursor. Furthermore, in this study, the medium-term predictions of the Ms7.3 Yutian, Ms8.0 Wenchuan, and Ms7.0 Lushan earthquakes are analytically presented and evaluated, especially to estimate location of earthquake. | Zhu Yiqing Liu Fang You Xinzhao Liang Weifeng Zhao Yunfeng Liu Lian | 2015 | Geodesy and Geodynamics2015，6，2： | 13 |
| 10 | Natural Variations at TIG1 Encoding a TCP Tran scription Factor Contribute to Plant Architecture Domestication in Rice显示文摘The modification of plant architecture is a crucial target in rice domestication and modern genetic improvement.Although several genes regulating rice plant architecture have been characterized,the molecular mechanisms underlying rice plant architecture domestication remain largely unclear.Here we show that the inclined tiller growth in wild rice is controlled by a single dominant gene,TILLER INCLINED GROWTH 1 (T/Gf),which is located on chromosome 8 and encodes a TCP transcriptional activator.TIG1 is primarily expressed in the adaxial side of the tiller base,promotes cell elongation,and enlarges the tiller angle in wild rice.Variations in the TIG1 promoter of indica cultivars {tig1 allele) resulted in decreased expression of TIG1 in the adaxial side of tiller base and reduced cell length and tiller angle,leading to the transition from inclined tiller growth in wild rice to erect tiller growth during rice domestication.TIG1 positively regulates the expression of EXP A3,EXPB5,and SAUR39 to promote cell elongation and increase the tiller angle.Selective sweep analysis revealed that the tig1 allele was selected in indica cultivars by human beings.The cloning and characterization of TIG1 supports a new scenario of plant architecture evolution in rice. | Weifeng Zhang Lubin Tan Hongying Sun Xinhui Zhao Fengxia Liu Hongwei Cai Yongcai Fu Xianyou Sun Ping Gu Zuofeng Zhu Chuanqing Sun | 2019 | Molecular Plant2019，12，8： | 13 |
| 11 | Drug nanoclusters formed in confined nano-cages of CD-MOF: dramatic enhancement of solubility and bioavailability of azilsartan显示文摘Tremendous efforts have been devoted to the enhancement of drug solubility using nanotechnologies, but few of them are capable to produce drug particles with sizes less than a few nanometers. This challenge has been addressed here by using biocompatible versatile γ-cyclodextrin(γ-CD) metal-organic framework(CD-MOF) large molecular cages in which azilsartan(AZL) was successfully confined producing clusters in the nanometer range. This strategy allowed to improve the bioavailability of AZL in Sprague–Dawley rats by 9.7-fold after loading into CD-MOF. The apparent solubility of AZL/CD-MOF was enhanced by 340-fold when compared to the pure drug. Based on molecular modeling, a dual molecular mechanism of nanoclusterization and complexation of AZL inside the CD-MOF cages was proposed, which was confirmed by small angle X-ray scattering(SAXS) and synchrotron radiation-Fourier transform infrared spectroscopy(SR-FTIR) techniques. In a typical cage-like unit of CD-MOF, three molecules of AZL were included by the γ-CD pairs, whilst other three AZL molecules formed a nanocluster inside the 1.7 nm sized cavity surrounded by six γ-CDs. This research demonstrates a dual molecular mechanism of complexation and nanoclusterization in CD-MOF leading to significant improvement in the bioavailability of insoluble drugs. | Yuanzhi He Wei Zhang Tao Guo Guoqing Zhang Wei Qin Liu Zhang Caifen Wang Weifeng Zhu Ming Yang Xiaoxiao Hu Vikramjeet Singh Li Wu Ruxandra Gref Jiwen Zhang | 2019 | Acta Pharmaceutica Sinica B2019，9，1： | 13 |
| 12 | Joint spatial registration and multi-target tracking using an extended PM-CPHD filter显示文摘An extended product multi-sensor cardinalized probability hypothesis density (PM-CPHD) filter for spatial registration and multi-target tracking (MTT) is proposed. The number and states of targets and the biases of sensors are jointly estimated by this method without the data association. Monte Carlo (MC) simulation results show that the proposed method (i) outperforms, although computationally more expensive than, the extended multi-sensor PHD filter which has been proposed for joint spatial registration and MTT; (ii) outperforms the multi-sensor joint probabilistic data association (MSJPDA) filter which is also extended in this study for joint spatial registration and MTT when the clutter is relatively dense. | LIAN Feng HAN ChongZhao LIU WeiFeng LIU Jing YUAN XiangHui | 2012 | Science China(Information Sciences)2012，55，3： | 11 |
| 13 | Propofol differentially induces unconsciousness and respiratory depression through distinct interactions between GABAa receptor and GABAergic neuron in corresponding nuclei显示文摘Propofol is the most commonly used intravenous anesthetic worldwide.It can induce loss of consciousness prior to the occurrence of severe respiratory suppression,which is also a pharmacodynamic feature of all general anesthetics.However,the neural mechanisms underlying this natural phenomenon are controversial and highly related to patient safety.In the present study,we demonstrated that the pharmacodynamic effects of propofol(50 and 100 μM)on suppression of consciousness-related excitatory postsynaptic currents in the medial prefrontal cortex(mPFC)and centromedian nucleus of the thalamus(CMT)were lower than those in the kernel respiratory rhythmogenesis nucleus pre-Botzinger complex(PrBo).Furthermore,we unexpectedly found that the GABAa receptor β3 subunit is the key target for propofol's action and that it is mutually and exclusively expressed in GABAergic neurons.It is also more abundant in the mPFC and CMT,but mainly co-localized with GABAergic neurons in the PrBo.As a result,the differentiated expression pattern should mediate more neuron suppression through the activation of GABAergic neurons in the mPFC and CMT at low doses of propofol(50 μM).However,PrBo GABAergic neurons were only activated by propofol at a high dose(100 μM).These results highlight the detailed pharmacodynamic effects of propofol on consciousness-related and respiration-related nuclei and provide the distinct interaction mechanism between the β3 subunit and GABAergic neurons in mediating the suppression of consciousness compared to the inhibition of respiration. | Junli Jiang Yingfu Jiao Po Gao Wen Yin Wei Zhou Yunchun Zhang Yanjun Liu Daxiang Wen Yuan Wang Liang Zhou Tian Yu Weifeng Yu | 2021 | Acta Biochimica et Biophysica Sinica2021，53，8： | 11 |
| 14 | Fluid-solid Interaction Model for Hydraulic Reciprocating O-ring Seals显示文摘Elastohydrodynamic lubrication characteristics of hydraulic reciprocating seals have significant effects on sealing and tribology performances of hydraulic actuators,especially in high parameter hydraulic systems.Only elastic deformations of hydraulic reciprocating seals were discussed,and hydrodynamic effects were neglected in many studies.The physical process of the fluid-solid interaction effect did not be clearly presented in the existing fluid-solid interaction models for hydraulic reciprocating O-ring seals,and few of these models had been simultaneously validated through experiments.By exploring the physical process of the fluid-solid interaction effect of the hydraulic reciprocating O-ring seal,a numerical fluid-solid interaction model consisting of fluid lubrication,contact mechanics,asperity contact and elastic deformation analyses is constructed with an iterative procedure.With the SRV friction and wear tester,the experiments are performed to investigate the elastohydrodynamic lubrication characteristics of the O-ring seal.The regularity of the friction coefficient varying with the speed of reciprocating motion is obtained in the mixed lubrication condition.The experimental result is used to validate the fluid-solid interaction model.Based on the model,The elastohydrodynamic lubrication characteristics of the hydraulic reciprocating O-ring seal are presented respectively in the dry friction,mixed lubrication and full film lubrication conditions,including of the contact pressure,film thickness,friction coefficient,liquid film pressure and viscous shear stress in the sealing zone.The proposed numerical fluid-solid interaction model can be effectively used to analyze the operation characteristics of the hydraulic reciprocating O-ring seal,and can also be widely used to study other hydraulic reciprocating seals. | LIAO Chuanjun HUANG Weifeng WANG Yuming SUO Shuangfu LIU Ying | 2013 | Chinese Journal of Mechanical Engineering2013，26，1： | 10 |
| 15 | Advanced rechargeable lithium-ion batteries based on bendable ZnCo2O4-urchins-on-carbon-fibers electrodes显示文摘ZnCo2O4-urchins-on-carbon-fibers 矩阵的一个新奇的班被设计了,描绘,并且过去常制作高效的精力存储设备。我们获得了 1180 妈数慲畴敲 ?L 癯 ? ㈱? 的一个可逆的锂存储能力 ?? 牣慥楳杮倠 ? 挠湯整瑮 ?? 桳牯 ? 整浲 ??? 琠敨浲污琠敲瑡敭瑮 ? 瑡 ??? 鲖 ? 栀氠睯牥愠摮甠灰牥焠慵瑮汩獥映牯搠楡祬洠湩浩浵琠浥数爔?爔 吗?? | Bin Liu Xianfu Wang Boyang Liu Qiufan Wang Dongsheng Tan Weifeng Song Xiaojuan Hou Di Chen Guozhen Shen | 2013 | Nano Research2013，6，7： | 10 |
| 16 | Timing of hydrothermal activity associated with the Douzhashan uranium-bearing granite and its significance for uranium mineralization in northeastern Guangxi, China显示文摘The Miaoershan uranium(U)ore field in northeastern Guangxi is one of the important granite-related U deposits in south China and is closely related to the Douzhashan U-bearing granite.The Douzhashan granite contains primary U-rich accessory minerals,including monazite(UO2=0.98-1.75 wt%)and xenotime(UO2=1.48-6.14 wt%).Primary monazite and xenotime yield chemical ages of 231±28 Ma and 230±38 Ma by electron microprobe analysis and U-Pb isotopic ages of 220±6 Ma and 211±7 Ma by laser ablation-inductively coupled-mass spectrometry respectively.These ages demonstrate that the Douzhashan granite formed during the period of Indosinian magmatic activity.Back scattered electron imaging shows that monazite and xenotime are commonly altered to assemblages of low-U synchisite and apatite,which was associated with loss of U to hydrothermal fluids.U-Th-Pb analyses of secondary apatite yielded a chemical age of 136±17 Ma,which corresponds to the timing of Cretaceous-Tertiary crustal extension in south China.We suggest that the heat and CO2required for mineralization was the result of Yanshanian crustal extension,and that this triggered the breakdown of U-rich accessory minerals in the Douzhashan U-bearing granite.Uranium remobilization from the Douzhashan granite provided materials for mineralization within the granite and/or surrounding country rocks.Therefore,a combination of Indosinian compression and Yanshanian extensional overprint produced the hydrothermal U deposits associated with the Douzhashan granite. | HU Huan WANG RuCheng CHEN WeiFeng CHEN PeiRong LING HongFei LIU GuoNing | 2013 | Chinese Science Bulletin2013，58，34： | 10 |
| 17 | Antidepressant-Like Action of Single Facial Injection of Botulinum Neurotoxin A is Associated with Augmented 5-HT Levels and BDNF/ERK/CREB Pathways in Mouse Brain显示文摘The present study was designed to examine the therapeutic effects of Botulinum neurotoxin A(BoNT/A)on depression-like behaviors in mice and to explore the potential mechanisms.These results revealed that a single facial injection of BoNT/A induced a rapid and prolonged improvement of depression-like behaviors in naive and space-restriction-stressed(SRS)mice,reflected by a decreased duration of immobility in behavioral despair tests.BoNT/A significantly increased the 5-hydroxytryptamine(5-HT)levels in several brain regions,including the hippocampus and hypothalamus,in SRS mice.BoNT/A increased the expression of the N-methyl-Daspartate receptor subunits NR1 and NR2 B in the hippocampus,which were significantly decreased in SRS mice.Furthermore,BoNT/A significantly increased the expression of brain-derived neurotrophic factor(BDNF)in the hippocampus,hypothalamus,prefrontal cortex,and amygdala,which were decreased in SRS mice.Finally,BoNT/A transiently increased the levels of phosphorylated extracellular signal-regulated kinase(p-ERK)and cAMPresponse element binding protein(p-CREB),which were suppressed in the hippocampus of SRS mice.Collectively,these results demonstrated that BoNT/A treatment has antidepressant-like activity in mice,and this is associated with increased 5-HT levels and the activation of BDNF/ERK/CREB pathways in the hippocampus,supporting further investigation of BoNT/A therapy in depression. | Yang Li Jing Liu Xu Liu Cun-Jin Su Qi-Lin Zhang Zhi-Hong Wang Lei-Fang Cao Xue-Yan Guo Ya Huang Weifeng Luo Tong Liu | 2019 | Neuroscience Bulletin2019，35，4： | 10 |
| 18 | Thermal Fluid-Solid Interaction Model and Experimental Validation for Hydrostatic Mechanical Face Seals显示文摘Hydrostatic mechanical face seals for reactor coolant pumps are very important for the safety and reliability of pressurized-water reactor power plants.More accurate models on the operating mechanism of the seals are needed to help improve their performance.The thermal fluid–solid interaction(TFSI)mechanism of the hydrostatic seal is investigated in this study.Numerical models of the flow field and seal assembly are developed.Based on the mechanism for the continuity condition of the physical quantities at the fluid–solid interface,an on-line numerical TFSI model for the hydrostatic mechanical seal is proposed using an iterative coupling method.Dynamic mesh technology is adopted to adapt to the changing boundary shape.Experiments were performed on a test rig using a full-size test seal to obtain the leakage rate as a function of the differential pressure.The effectiveness and accuracy of the TFSI model were verified by comparing the simulation results and experimental data.Using the TFSI model,the behavior of the seal is presented,including mechanical and thermal deformation,and the temperature field.The influences of the rotating speed and differential pressure of the sealing device on the temperature field,which occur widely in the actual use of the seal,are studied.This research proposes an on-line and assembly-based TFSI model for hydrostatic mechanical face seals,and the model is validated by full-sized experiments. | HUANG Weifeng LIAO Chuanjun LIU Xiangfeng SUO Shuangfu LIU Ying WANG Yuming | 2014 | Chinese Journal of Mechanical Engineering2014，27，5： | 10 |
| 19 | Phylogenetics of varied subtypes of avian influenza viruses in China: potential threat to humans显示文摘 | Weifeng Shi Wei Li Xianbin Li Joel Haywood Juncai Ma George F.Gao Di Liu | 2014 | Protein & Cell2014，5，4： | 9 |
| 20 | A two-step lineage reprogramming strategy to generate functionally competent human hepatocytes from fibroblasts显示文摘Terminally differentiated cells can be generated by lineage reprogramming,which is,however,hindered by incomplete conversion with residual initial cell identity and partial functionality. Here, we demonstrate a new reprogramming strategy by mimicking the natural regeneration route, which permits generating expandable hepatic progenitor cells and functionally competent human hepatocytes. Fibroblasts were first induced into human hepatic progenitor-like cells (hHPLCs), which could robustly expand in vitro and efficiently engraft in vivo. Moreover, hHPLCs could be efficiently induced into mature human hepatocytes (hiHeps) in vitro, whose molecular identity highly resembles primary human hepatocytes (PHHs). Most importantly, hiHeps could be generated in large quantity and were functionally competent to replace PHHs for drug-metabolism estimation, toxicity prediction and hepatitis B virus infection modeling. Our results highlight the advantages of the progenitor stage for successful lineage reprogramming. This strategy is promising for generating other mature human cell types by lineage reprogramming. | Bingqing Xie Da Sun Yuanyuan Du Jun Jia Shicheng Sun Jun Xu Yifang Liu Chengang Xiang Sitong Chen Huangfan Xie Qiming Wang Guangya Li Xuehui LYU Hui Shen Shiyu Li Min Wu Xiaonan Zhang Yue Pu Kuanhui Xiang Weifeng Lai Peng Du Zhenghong Yuan Cheng Li Yan Shi Shichun Lu Hongkui Deng | 2019 | Cell Research2019，29，9： | 9 |
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