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| 1 | The first offshore natural gas hydrate production test in South China Sea显示文摘Natural gas hydrates (NGH)is one of key future clean energy resources.Its industrialized development will help remit the huge demand of global natural gas,relieve the increasing pressure of the environment, and play a vital role in the green sustainable growth of human societies.Based on nearly two decades' studying on the reservoir characteristics in the South China Sea (SCS)and the knowledge of reservoir system,the China Geological Survey (CGS)conducted the first production test on an optimal target selected in Shenhu area SCS in 2017.Guided by the 'three-phase control'exploitation theory which focused on formation stabilization,technologies such as formation fluid extraction,well drilling and completing, reservoir stimulating,sand controlling,environmental monitoring,monitoring and preventing of secondary formation of hydrates were applied.The test lasted for 60 days from May 10^th when starting to pump, drop pressure and ignite to well killing on July 9^th,with gas production of 3.09×10^5 m^3 in total,which is a world record with the longest continuous duration of gas production and maximal gas yield.This successful test brings a significant breakthrough on safety control of NGH production. | Jin-fa Li Jian-liang Ye Xu-wen Qin Hai-jun Qiu Neng-you Wu Hai-long Lu Wen-wei Xie Jing-an Lu Fei Peng Zhen-qiang Xu Cheng Lu Zeng-gui Kuang Jian-gong Wei Qian-yong Liang Hong-feng Lu Bei-bei Kou | 2018 | China Geology2018，1，1： | 117 |
| 2 | 2019新型冠状病毒基因组特征和流行病学:病毒起源和受体结合的意义显示文摘研究者对来自9例新型冠状病毒肺炎住院患者的支气管肺泡灌洗液样本和培养的分离株进行了下一代测序。从这些个体中获得了严重急性呼吸综合征-冠状病毒2(severe acute respiratory syndrome-coronavirus 2,SARS-CoV-2)的完整和部分基因组序列。利用Sanger测序连接病毒重叠群以获得全长基因组,cDNA末端快速扩增确定终端区。对这些SARSCoV-2基因组和其他冠状病毒基因组进行了系统进化分析,以确定该病毒的进化史并有助于推断其可能的起源。 | 刘青(译) 刘莉(审校) Lu R Zhao X Li J Niu P Yang B Wu H Wang W Song H Huang B Zhu N Bi Y Ma X Zhan F Wang L Hu T Zhou H Hu Z Zhou W Zhao L Chen J Meng Y Wang J Lin Y Yuan J Xie Z Ma J Liu WJ Wang D Xu W Holmes EC Gao GF Wu G Chen W Shi W Tan W | 2020 | 中华高血压杂志2020，28，3： | 516 |
| 3 | Clinical Features of Adult/Adolescent Atopic Dermatitis and Chinese Criteria for Atopic Dermatitis显示文摘 | Ping Liu Yan Zhao Zhang-Lei Mu Qian-Jin Lu Qian-Jin L U Li Zhang Xu Yao Min Zheng Yi-Wen Tang Xin-Xiano Lu Xiu-Juan xia You-Kun Lin Yu-Zhen Li Cai-Xia Tu Zhi-Rong Yao Jin-Hua Xu Wei Li Wei Lai Hui-Min Yang Hong-Fu Xie Xiu-Ping Han Zhi-Qiang Xie Xiang Nong Zai-Pei Guo Dan-Qi Deng Tong-Xin Shi Jian-Zhong Zhang | 2016 | Chinese Medical Journal2016，，7： | 64 |
| 4 | Diagnosis, treatment, and prevention of 2019 novel coronavirus infection in children: experts' consensus statement显示文摘Since the outbreak of 2019 novel coronavirus infection (2019-nCoV) in Wuhan City,China,by January 30,2020,a total of 9692 confirmed cases and 15,238 suspected cases have been reported around 31 provinces or cities in China.Among the confirmed cases,1527 were severe cases,171 had recovered and been discharged at home,and 213 died.And among these cases,a total of 28 children aged from 1 month to 17 years have been reported in China.For standardizing prevention and management of 2019-nCoV infections in children,we called up an experts' committee to formulate this experts' consensus statement.This statement is based on the Novel Coronavirus Infection Pneumonia Diagnosis and Treatment Standards (the fourth edition) (National Health Committee) and other previous diagnosis and treatment strategies for pediatric virus infections.The present consensus statement summarizes current strategies on diagnosis,treatment,and prevention of 2019-nCoV infection in children. | Kunling Shen Yonghong Yang Tianyou Wang Dongchi Zhao Yi Jiang Runming Jin Yuejie Zheng Baoping Xu Zhengde Xie Likai Lin Yunxiao Shang Xiaoxia Lu Sainan Shu Yan Bai Jikui Deng Min Lu Leping Ye Xuefeng Wang Yongyan Wang Liwei Gao | 2020 | World Journal of Pediatrics2020，16，3： | 56 |
| 5 | Visualization of the complex structure and stress field inside rock by means of 3D printing technology显示文摘Accurate characterization and visualization of the complex inner structure and stress distribution of rocks are of vital significance to solve a variety of underground engineering problems. In this paper, we incorporate several advanced technologies, such as CT scan, three-dimensional(3D) reconstruction, and 3D printing, to produce a physical model representing the natural coal rock that inherently contains complex fractures or joints. We employ 3D frozen stress and photoelastic technologies to characterize and visualize the stress distribution within the fractured rock under uniaxial compression. The 3D printed model presents the fracture structures identical to those of the natural prototype. The mechanical properties of the printed model,including uniaxial compression strength, elastic modulus,and Poisson's ratio, are testified to be similar to those of the prototype coal rock. The frozen stress and photoelastic tests show that the location of stress concentration and the stress gradient around the discontinuous fractures are in good agreement with the numerical predictions of the real coalsample. The proposed method appears to be capable of visually quantifying the influences of discontinuous,irregular fractures on the strength, deformation, and stress concentration of coal rock. The method of incorporating3 D printing and frozen stress technologies shows a promising way to quantify and visualize the complex fracture structures and their influences on 3D stress distribution of underground rocks, which can also be used to verify numerical simulations. | Yang Ju Heping Xie Zemin Zheng Jinbo Lu Lingtao Mao Feng Gao Ruidong Peng | 2014 | Chinese Science Bulletin2014，59，36： | 41 |
| 6 | Clinical analysis and follow-up study of chronic active Epstein-Barr virus infection in 53 pediatric cases显示文摘背景长期的活跃 Epstein-Barr 病毒感染(CAEBV ) 以前被报导了有时与一堂好攻击的临床的功课被联系。在大陆中国小儿科的病人的 CAEBV 的特征大部分是 unreported。这调查的主要目的是在孩子认出 CAEBV 的临床的特征并且探索它的诊断标准和风险 factors.Methods A 回顾的学习与 CAEBV 在 53 个小儿科的病人(36 个男孩和 17 个女孩) 上被执行进入北京孩子在 2003 和 2007 之间的医院。所有他们的医药记录被考察并且分析。为每个病人,人口统计,临床,实验室数据和结果被收集。独立样品的 t 测试被用于在 CAEBV 的发作的年龄从 2 个月到 14.6 年的统计 analysis.Results (平均数(5. | LU Gen XIE Zheng-de ZHAO Shun-ying YE Ling-jun WU Run-hui LIU Chun-yan YANG Shuang JIN Ying-kang SHEN Kun-ling | 2009 | Chinese Medical Journal2009，，3： | 36 |
| 7 | Natural variation in Ghd7.1 plays an important role in grain yield and adaptation in rice显示文摘 | Wenhao Yan Haiyang Liu Xiangchun Zhou Qiuping Li Jia Zhang Li Lu Touming Liu Haijun Liu Chengjun Zhang Zhanyi Zhang Guojing Shen Wen Yao Huaxia Chen Sibin Yu Weibo Xie Yongzhong Xing | 2013 | Cell Research2013，23，7： | 39 |
| 8 | AXL is a candidate receptor for SARS-CoV-2 that promotes infection of pulmonary and bronchial epithelial cells显示文摘The current coronavirus disease 2019(COVID-19)pandemic presents a global public health challenge.The viral pathogen responsible,severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),binds to the host receptor ACE2 through its spike(S)glycoprotein,which mediates membrane fusion and viral entry.Although the role of ACE2 as a receptor for SARS-CoV-2 is clear,studies have shown that ACE2 expression is extremely low in various human tissues,especially in the respiratory tract.Thus,other host receptors and/or co-receptors that promote the entry of SARS-CoV-2 into cells of the respiratory system may exist.In this study,we found that the tyrosine-protein kinase receptor UFO(AXL)specifically interacts with the N-terminal domain of SARS-CoV-2 S.Using both a SARS-CoV-2 virus pseudotype and authentic SARS-CoV-2,we found that overexpression of AXL in HEK293T cells promotes SARS-CoV-2 entry as efficiently as overexpression of ACE2,while knocking out AXL significantly reduces SARS-CoV-2 infection in HI 299 pulmonary cells and in human primary lung epithelial cells.Soluble human recombinant AXL blocks SARS-CoV-2 infection in cells expressing high levels of AXL.The AXL expression level is well correlated with SARS-CoV-2 S level in bronchoalveolar lavage fluid cells from COVID-19 patients.Taken together,our findings suggest that AXL is a novel candidate receptor for SARS-CoV-2 which may play an important role in promoting viral infection of the human respiratory system and indicate that it is a potential target for future clinical intervention strategies. | Shuai Wang Zongyang Qiu Yingnan Hou Xiya Deng Wei Xu Tingting Zheng Peihan Wu Shaofang Xie Weixiang Bian Chong Zhang Zewei Sun Kunpeng Liu Chao Shan Aifu Lin Shibo Jiang Youhua Xie Qiang Zhou Lu Lu Jing Huang Xu Li | 2021 | Cell Research2021，31，2： | 39 |
| 9 | The First Imported Case of Monkeypox in the Mainland of China—Chongqing Municipality,China,September 16,2022显示文摘Monkeypox is a zoonotic viral disease caused by the monkeypox virus(MPXV),and historically,all outbreaks have been linked to Africa;however,monkeypox has been posing an alarming challenge to the world in 2022(1)as approximately 60,000 cases have been reported in more than 100 nations and regions worldwide(2).Currently,many cases of monkeypox were identified in many nonendemic countries outside of Central and West Africa,and human-to-human transmission has occurred frequently,especially among men who have sex with men(MSM)presenting new clinical symptoms similar to syphilis and other sexually transmitted infections(3). | Hua Zhao Wenling Wang Li Zhao Sheng Ye Jingdong Song Roujian Lu Hua Zong Changcheng Wu Wei Huang Baoying Huang Yao Deng Ruhan A Wujuan Xie Li Qi Wenbo Xu Hua Ling Wenjie Tan | 2022 | China CDC weekly2022，4，38： | 35 |
| 10 | Caspase-11/4 and gasdermin D-mediated pyroptosis contributes to podocyte injury in mouse diabetic nephropathy显示文摘Diabetic nephropathy(DN)is characterized by sterile inflammation with continuous injury and loss of renal inherent parenchyma cells.Podocyte is an essential early injury target in DN.The injury and loss of podocytes are closely associated with proteinuria,the early symptom of renal injury in DN.However,the exact mechanism for podocyte injury and death in DN remains ambiguous.In this study we investigated whether pyroptosis,a newly discovered cell death pathway was involved in DN.Diabetic mice were generated by high-fat diet/STZ injections.We showed that the expression levels of caspase-11 and cleavage of gasdermin D(GSDMD-N)in podocytes were significantly elevated,accompanied by reduced expression of podocyte makers nephrin and podocin,loss and fusion in podocyte foot processes,increased inflammatory cytokines NF-κB,IL-1β,and IL-18,macrophage infiltration,glomerular matrix expansion and increased urinary albumin to creatinine ratio(UACR).All these changes in diabetic mice were blunted by knockout of caspase-11 or GSDMD.Cultured human and mouse podocytes were treated with high glucose(30 mM),which significantly increased the expression levels of caspase-11 or caspase-4(the homolog of caspase-11 in human),GSDMD-N,NF-κB,IL-1β,and IL-18,and decreased the expression of nephrin and podocin.Either caspase-4 or GSDMD knockdown by siRNA significantly blunted these changes.In summary,our results demonstrate that caspase-11/4 and GSDMD-mediated pyroptosis is activated and involved in podocyte loss under hyperglycemia condition and the development of DN. | Qian Cheng Jing Pan Zhuan-li Zhou Fan Yin Hong-yan Xie Pan-pan Chen Jing-yao Li Pei-qing Zheng Li Zhou Wei Zhang Jun Liu Li-min Lu | 2021 | Acta Pharmacologica Sinica2021，42，6： | 33 |
| 11 | Epstein-Barr virus-associated hemophagocytic lymphohistiocytosis: a retrospective study of 78 pediatric cases in mainland of China显示文摘背景 Epstein-Barr 联系病毒的 hemophagocytic lymphohistiocytosis (EBV-HLH ) 的临床的特征大部分是在在中国的大陆的小儿科的病人的 unreported。这研究的主要目的是在孩子认出 EBV-HLH 的临床的特征并且探索它的预后和风险 factors.Methods A 回顾的学习与 EBV-HLH 在 78 个小儿科的病人上被执行进入北京孩子在 2003 和 2008 之间的医院。所有病人的医药记录被考察并且分析。为每个病人,人口统计,临床,实验室和结果信息被收集。统计分析经由 multivariate 和 univariate analysis.Results 被进行发作的年龄在 1-2 年之间达到顶点,男孩们是更多半发达的 EBV-HLH。EBV-HLH 与 EBV 原子抗原(EBNA ) 主要在血清学的模式发生了积极(70.5%) 。疾病的全面命运是 56.7% 。39 死亡(30.8%) 中的 12 个在诊断以后在 2 个月以内很快死了。 Multivariate 分析揭示了那不收到化疗( P=0.002 ), 4 星期在诊断( P=0.004 )以前的病,并且白朊铺平< 20 g/L ( P=0.045 )显著地预言增加的命运 risk.Conclusions EBV-HLH 是有与 EBNA 主要在血清学的模式发生的高命运率的严重疾病积极。化疗和及时诊断的早开始显著地改进幸存率。实际策略应该集中于减少早死亡的可能性的。 | JIN Ying-kang XIE Zheng-de YANG Shuang LU Gen SHEN Kun-ling | 2010 | Chinese Medical Journal2010，，11： | 30 |
| 12 | Long-Term Effect of Fertilizer and Rice Straw on Mineral Composition and Potassium Adsorption in a Reddish Paddy Soil显示文摘Increasing K+ adsorption can be an effective alternative in building an available K pool in soils to optimize crop recovery and minimize losses into the environment. We hypothesized that long-term fertilization might change K+ adsorption because of changes in the chemical and mineralogical properties of a rice (Oryza sativa L.). The aims of this study were (i) to determine clay minerals in paddy soil clay size fractions using X-ray diffraction methods and a numerical diagram-decomposition method; (ii) to measure K+ adsorption isotherms before and after H 2 O 2 oxidation of organic matter, and (iii) to investigate whether K+ adsorption is correlated with changes in soil chemical and mineral properties. The 30-yr long-term fertilization treatments caused little change in soil organic C (SOC) but a large variation in soil mineral composition. The whole-clay fraction (<5 μm) corresponded more to the fertilization treatment than the fine-clay fraction (<1 μm) in terms of percentage of illite peak area. The total percentage of vermiculite-chlorite peak area was significantly negatively correlated with the total percentage of illite peak area in the <5 μm soil particles (R=-0.946, P<0.0006). Different fertilization treatments gave significantly different results in K+ adsorption. The SOC oxidation test showed positive effects of SOC on K+ adsorption at lower K+ concentration ( 120 mg L-1) and negative effects at higher K+ concentration (240 mg L-1). The K+ adsorption by soil clay minerals after SOC oxidization accounted for 60-158% of that by unoxidized soils, suggesting a more important role of soil minerals than SOC on K+ adsorption. The K+ adsorption potential was significantly correlated to the amount of poorly crystallized illite present (R=0.879, P=0.012). The availability of adsorbed K+ for plant growth needs further study. | LIAO Yu-lin ZHENG Sheng-xian NIE Jun XIE Jian LU Yan-hong QIN Xiao-bo | 2013 | Journal of Integrative Agriculture2013，12，4： | 30 |
| 13 | PVT v2:Improved baselines with Pyramid Vision Transformer显示文摘Transformers have recently lead to encouraging progress in computer vision.In this work,we present new baselines by improving the original Pyramid Vision Transformer(PVT v1)by adding three designs:(i)a linear complexity attention layer,(ii)an overlapping patch embedding,and(iii)a convolutional feed-forward network.With these modifications,PVT v2 reduces the computational complexity of PVT v1 to linearity and provides significant improvements on fundamental vision tasks such as classification,detection,and segmentation.In particular,PVT v2 achieves comparable or better performance than recent work such as the Swin transformer.We hope this work will facilitate state-ofthe-art transformer research in computer vision.Code is available at http://gffzz188fe103f8f1460ask6nwpxvu9qcu6wcf.ffgz.tsg.suse.edu.cn/whai362/PVT. | Wenhai Wang Enze Xie Xiang Li Deng-Ping Fan Kaitao Song Ding Liang Tong Lu Ping Luo Ling Shao | 2022 | Computational Visual Media2022，8，3： | 30 |
| 14 | Dual gRNAs guided CRISPR/Cas9 system inhibits hepatitis B virus replication显示文摘AIM: To screen and investigate the effective g RNAs against hepatitis B virus(HBV) of genotypes A-D.METHODS: A total of 15 g RNAs against HBV of genotypes A-D were designed. Eleven combinations of two above g RNAs(dual-g RNAs) covering the regulatory region of HBV were chosen. The efficiency of each g RNA and 11 dual-g RNAs on the suppression of HBV(genotypes A-D) replication was examined by the measurement of HBV surface antigen(HBs Ag) or e antigen(HBe Ag) in the culture supernatant. The destruction of HBV-expressing vector was examined in Hu H7 cells co-transfected with dual-g RNAs and HBVexpressing vector using polymerase chain reaction(PCR) and sequencing method, and the destruction of ccc DNAwas examined in Hep AD38 cells using KCl precipitation, plasmid-safe ATP-dependent DNase(PSAD) digestion, rolling circle amplification and quantitative PCR combined method. The cytotoxicity of these g RNAs was assessed by a mitochondrial tetrazolium assay.RESULTS: All of g RNAs could significantly reduce HBs Ag or HBe Ag production in the culture supernatant, which was dependent on the region in which g RNA against. All of dual g RNAs could efficiently suppress HBs Ag and/or HBe Ag production for HBV of genotypes A-D, and the efficacy of dual g RNAs in suppressing HBs Ag and/or HBe Ag production was significantly increased when compared to the single g RNA used alone. Furthermore, by PCR direct sequencing we confirmed that these dual g RNAs could specifically destroy HBV expressing template by removing the fragment between the cleavage sites of the two used g RNAs. Most importantly, g RNA-5 and g RNA-12 combination not only could efficiently suppressing HBs Ag and/or HBe Ag production, but also destroy the ccc DNA reservoirs in Hep AD38 cells.CONCLUSION: These results suggested that CRISPR/Cas9 system could efficiently destroy HBV expressing templates(genotypes A-D) without apparent cytotoxicity. It may be a potential approach for eradication of persistent HBV ccc DNA in chronic HBV infection patients. | Jie Wang Zhong-Wei Xu Shuang Liu Rui-Yang Zhang Shan-Long Ding Xiao-Meng Xie Lu Long Xiang-Mei Chen Hui Zhuang Feng-Min Lu | 2015 | World Journal of Gastroenterology2015，21，32： | 29 |
| 15 | Genome-wide identification of CRISPR/Cas9 off-targets in human genome显示文摘 | Jinzhi Duan Guangqing Lu Zhan Xie Mingliang Lou Jiao Luo Lei Guo Yu Zhang | 2014 | Cell Research2014，24，8： | 27 |
| 16 | Digestive tract reconstruction using isoperistaltic jejunumlater-cut overlap method after totally laparoscopic total gastrectomy for gastric cancer: Short-term outcomes and impact on quality of life显示文摘AIM To evaluate the short-term outcomes and quality of life(Qo L) in gastric cancer patients undergoing digestive tract construction using the isoperistaltic jejunum-latercut overlap method(IJOM) after totally laparoscopic total gastrectomy(TLTG).METHODS A total of 507 patients who underwent laparoscopic gastrectomy(D2) from January 2014 to March 2016 were originally included in the study. The patients were divided into two groups to undergo digestive tract construction using either IJOM after TLTG(group T, n = 51) or Roux-en-Y anastomosis after laparoscopic-assisted total gastrectomy(LATG)(group A, n = 456). The short-term outcomes and Qo L were compared between the two groups after 1:2 propensity-score matching(PSM). We used a questionnaire to assess Qo L.RESULTS Before matching, age, sex, tumor size, tumor location, preoperative albumin and blood loss were significantly different between the two groups(P < 0.05). After PSM, the patients were well balanced in terms of their clinicopathological characteristics, although both blood loss and in-hospital postoperative days in group T were significantly lower than those in group A(P < 0.05). After matching, group T reported better Qo L in the domains of pain and dysphagia. Among the items evaluating pain and dysphagia, group T tended to report better Qo L('Have you felt pain' and 'Have you had difficulty eating solid food')(P < 0.05).CONCLUSION The IJOM for digestive tract reconstruction after TLTG is associated with reduced blood loss and less pain and dysphagia, thus improving Qo L after laparoscopic gastrectomy. | Ze-Ning Huang Chang-Ming Huang Chao-Hui Zheng Ping Li Jian-Wei Xie Jia-Bin Wang Jian-Xian Lin Jun Lu Qi-Yue Chen Long-Long Cao Mi Lin Ru-Hong Tu Ju-Li Lin | 2017 | World Journal of Gastroenterology2017，23，39： | 27 |
| 17 | Practice guidelines for ultrasound-guided percutaneous microwave ablation for hepatic malignancy显示文摘Primary liver cancer and liver metastases are among the most frequent malignancies worldwide,with an increasing number of new cases and deaths every year.Traditional surgery is only suitable for a limited proportion of patients and imaging-guided percutaneous thermal ablation has achieved optimistic results for management of hepatic malignancy.This synopsis outlines the first clinical practice guidelines for ultrasoundguided percutaneous microwave ablation therapy for hepatic malignancy,which was created by a joint task force of the Society of Chinese Interventional Ultrasound.The guidelines aim at standardizing the microwave ablation procedure and therapeutic efficacy assessment,as well as proposing the criteria for the treatment candidates. | Ping Liang Jie Yu Ming-De Lu Bao-Wei Dong Xiao-Ling Yu Xiao-Dong Zhou Bing Hu Ming-Xing Xie Wen Cheng Wen He Jian-Wen Jia Guo-Rong Lu | 2013 | World Journal of Gastroenterology2013，19，33： | 26 |
| 18 | pORF5 plasmid protein of Chlamydia trachomatis induces MAPK-mediated pro-inflammatory cytokines via TLR2 activation in THP-1 cells显示文摘Infection with Chlamydia trachomatis induces inflammatory pathologies in the urogenital tract that can lead to infertility and ectopic pregnancy.Pathogenesis of infection has been mostly attributed to excessive cytokine production.However,precise mechanisms on how C.trachomatis triggers this production,and which protein(s) stimulate inflammatory cytokines remains unknown.In the present study,the C.trachomatis pORF5 protein induced tumor necrosis factor alpha(TNF-α),interleukin-1 beta(IL-1β) and interleukin-8(IL-8) in dose-and time-dependent manners in the THP-1 human monocyte cell line.We found that intracellular p38/mitogen-activated protein kinase(MAPK) and extracellular signal-regulated kinase(ERK)/MAPK signaling pathways were required for the induction of TNF-α,IL-1β and IL-8.Blockade of toll-like receptor 2(TLR2) signaling reduced induction levels of TNF-α,IL-8 and IL-1β.We concluded that the C.trachomatis pORF5 protein might contribute to the inflammatory processes associated with chlamydial infections. | ZHOU Hui HUANG QiuLin LI ZhongYu WU YiMou XIE XiaoBing MA KangKang CAO WenJuan ZHOU Zhou LU ChunXue ZHONG GuangMing | 2013 | Science China(Life Sciences)2013，56，5： | 26 |
| 19 | Comparision of modified and conventional delta-shaped gastroduodenostomy in totally laparoscopic surgery显示文摘AIM:To evaluate the safety and feasibility of a modified delta-shaped gastroduodenostomy(DSG)in totally laparoscopic distal gastrectomy(TLDG).METHODS:We performed a case-control study enrolling 63 patients with distal gastric cancer(GC)undergoing TLDG with a DSG from January 2013 to June 2013.Twenty-two patients underwent a conventional DSG(Con-Group),whereas the other 41 patients underwent a modified version of the DSG(Mod-Group).The modified procedure required only the instruments of the surgeon and assistant to complete the involution of the common stab incision and to completely resect the duodenal cutting edge,resulting in an anastomosis with an inverted T-shaped appearance.The clinicopathological characteristics,surgical outcomes,anastomosis time and complications of the two groups were retrospectively analyzed using a prospectively maintained comprehensive database.RESULTS:DSG procedures were successfully completed in all of the patients with histologically complete(R0)resections,and none of these patients required conversion to open surgery.The clinicopathological characteristics of the two groups were similar.There were no significant differences between the groups in the operative time,intraoperative blood loss,extension of the lymph node(LN)dissection and number of dissected LNs(150.8±21.6 min vs 143.4±23.4 min,P=0.225for the operative time;26.8±11.3 min vs 30.6±14.8mL,P=0.157 for the intraoperative blood loss;4/18 vs3/38,P=0.375 for the extension of the LN dissection;and 43.9±13.4 vs 39.5±11.5 per case,P=0.151 for the number of dissected LNs).The anastomosis time,however,was significantly shorter in the Mod-Group than in the Con-Group(13.9±2.8 min vs 23.9±5.6min,P=0.000).The postoperative outcomes,including the times to out-of-bed activities,first flatus,resumption of soft diet and postoperative hospital stay,as well as the anastomosis size,did not differ significantly(1.9±0.6 d vs 2.3±1.5 d,P=0.228 for the time to outof-bed activities;3.2±0.9 d vs 3.5±1.3 d,P=0.295for the first flatus time;7.5±0.8 d vs 8.1±4.3 d,P=0.489 for the resumption of a soft diet time;14.3±10.6 d vs 11.5±4.9 d,P=0.148 for the postoperative hospital stay;and 30.5±3.6 mm vs 30.1±4.0 mm,P=0.730 for the anastomosis size).One patient with minor anastomotic leakage in the Con-Group was managed conservatively;no other patients experienced any complications around the anastomosis.The operative complication rates were similar in the Con-and ModGroups(9.1%vs 7.3%,P=1.000).CONCLUSION:The modified DSG,an alternative reconstruction in TLDG for GC,is technically safe and feasible,with a simpler process that reduces the anastomosis time. | Chang-Ming Huang Mi Lin Jian-Xian Lin Chao-Hui Zheng Ping Li Jian-Wei Xie Jia-Bin Wang Jun Lu | 2014 | World Journal of Gastroenterology2014，20，30： | 26 |
| 20 | Ketogenic diet poses a significant effect on imbalanced gut microbiota in infants with refractory epilepsy显示文摘AIM To investigate whether patients with refractory epilepsy and healthy infants differ in gut microbiota(GM),and how ketogenic diet(KD) alters GM.METHODS A total of 14 epileptic and 30 healthy infants were recruited and seizure frequencies were recorded. Stool samples were collected for 16 S r DNA sequencing using the Illumina Miseq platform. The composition of GM in each sample was analyzed with MOTHUR,and intergroup comparison was conducted by R software.RESULTS After being on KD treatment for a week,64% of epileptic infants showed an obvious improvement,with a 50% decrease in seizure frequency. GM structure in epileptic infants(P1 group) differed dramatically from that in healthy infants(Health group). Proteobacteria,which had accumulated significantly in the P1 group,decreased dramatically after KD treatment(P2 group). Cronobacter predominated in the P1 group and remained at a low level both in the Health and P2 groups. Bacteroides increased significantly in the P2 group,in which Prevotella and Bifidobacterium also grew in numbers and kept increasing.CONCLUSION GM pattern in healthy infants differed dramatically from that of the epileptic group. KD could significantly modify symptoms of epilepsy and reshape the GM of epileptic infants. | Gan Xie Qian Zhou Chuang-Zhao Qiu Wen-Kui Dai He-Ping Wang Yin-Hu Li Jian-Xiang Liao Xin-Guo Lu Su-Fang Lin Jing-Hua Ye Zhuo-Ya Ma Wen-Jian Wang | 2017 | World Journal of Gastroenterology2017，23，33： | 25 |
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