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| 1 | aunalysis of the Genome Sequence of the Medicinal Plant Salvia miltiorrhiza显示文摘 | Haibin Xu Jingyuan Song Hongmei Luo Yujun Zhang Qiushi Li Yingjie Zhu Jiang Xu Ying Li Chi Song Bo Wang Wei Sun Guoan Shen Xin Zhang Jun Qian Aijia Ji Zhichao Xu Xiang Luo Liu He Chuyuan Li Chao Sun Haixia Yah Guanghong Cui Xiwen Li Xian 'en Li Jianhe Wei Juyan Liu Yitao Wang Alice Hayward David Nelson Zemin Ning Reuben J. Peters Xiaoquan Qi Shilin Chen | 2016 | Molecular Plant2016，9，6： | 70 |
| 2 | The willow genome and divergent evolution from poplar after the common genome duplication显示文摘 | Xiaogang Dai Quanjun Hu Qingle Cai Kai Feng Ning Ye Gerald A Tuskan Richard Milne Yingnan Chen Zhibing Wan Zefu Wang Wenchun Luo Kun Wang Dongshi Wan Mingxiu Wang Jun Wang Jianquan Liu Tongming Yin | 2014 | Cell Research2014，24，10： | 28 |
| 3 | Fibrinogen-like protein 2 fibroleukin expression and its correlation with disease progression in murine hepatitis virus type 3-induced fulminant hepatitis and in patients with severe viral hepatitis B显示文摘AIM: To evaluate the expression of fibrinogenlike protein 2 (fgl2) and its correlation with disease progression in both mice and patients with severe viral hepatitis.METHODS: Balb/cJ or A/J mice were infected intraperitoneally (ip) with 100 PFU of murine hepatitis virus type 3 (MHV-3), liver and serum were harvested at 24, 48, and 72 h post infection for further use. Liver tissues were obtained from 23 patients with severe acute chronic (AOC) hepatitis B and 13 patients with mild chronic hepatitis B. Fourteen patients with mild chronic hepatitis B with cirrhosis and 4 liver donors served as normal controls. In addition, peripheral blood mononuclear cells (PBMC) were isolated from 30 patients (unpaired) with severe AOC hepatitis B and 10 healthy volunteers as controls. Procoagulant activity representing functional prothrombinaseactivity in PBMC and white blood cells was also assayed. A polyclonal antibody against fgl2 was used to detect the expression of both mouse and human fgl2 protein in liver samples as well as in PBMC by immunohistochemistry staining in a separate set of studies. Alanine aminotransferase (ALT) and total bilirubin (TBil) in serum were measured to assess the severity of liver injury.RESULTS: Histological changes were found in liver sections 12-24 h post MHV-3 infection in Balb/cJ mice.In association with changes in liver histology, marked elevations in serum ALT and TBil were observed. Mouse fgl2 (mfgl2) protein was detected in the endothelium of intrahepatic veins and hepatic sinusoids within the liver 24 h after MHV-3 infection. Liver tissues from the patients with severe AOC hepatitis B had classical pathological features of acute necroinflammation. Human fgl2 (hfgl2)was detected in 21 of 23 patients (91.30%)with severe AOC hepatitis B, while only 1 of 13 patients(7.69%) with mild chronic hepatitis B and cirrhosis had hfgl2 mRNA or protein expression. Twenty-eight of thirty patients (93.33%) with severe AOC hepatitis B and 1of 10 with mild chronic hepatitis B had detectable hfgl2expression in PBMC. No hfgl2 expression was found either in the liver tissue or in the PBMC from normal donors. There was a positive correlation between hfgl2expression and the severity of the liver disease as indicated by the levels of TBil. PCA significantly increased in PBMC in patients with severe AOC hepatitis B.CONCLUSION: The molecular and cellular results reported here in both mice and patients with severe viral hepatitis suggest that virus-induced hfgl2prothrombinase/fibroleukin expression and the coagulation activity associated with the encoded fgl2protein play a pivotal role in initiating severe hepatitis.The measurement of hfgl2/fibroleukin expression in PBMC may serve as a useful marker to monitor the severity of AOC hepatitis B and a target for therapeutic intervention. | Chuan-Long Zhu Wei-Ming Yan Fan Zhu Yong-Fen Zhu Dong Xi De-Ying Tian Gary Levy Xiao-Ping Luo Qin Ning | 2005 | World Journal of Gastroenterology2005，11，44： | 26 |
| 4 | Influence of temperature and solution composition on the formation of calcium sulfates显示文摘Calcium sulfates (anhydrite and hydrates) were synthesized by mixing CaCl 2 and Na 2 SO 4 solutions at room temperature followed by aging the resulting slurries at elevated temperatures. The variation of the morphology and structure of the calcium sulfates with aging temperature was investigated. Experimental results indicated that CaSO 4 ·2H 2 O plates, CaSO 4 ·0.5H 2 O whiskers and CaSO 4 spindles were formed at ≤100 °C, 130–160 °C and ≥170 °C, respectively. The formation and conversion of the calcium sulfates were discussed on the basis of characterization of the products and chemical analysis of the solutions. Compared to NaCl solution, pure water favors one-dimensional hydrothermal growth of CaSO 4 ·0.5H 2 O whiskers owing to lower supersaturation. | Kangbi Luo Chunmei Li Lan Xiang Huping Li Ping Ning | 2010 | Particuology2010，8，3： | 26 |
| 5 | MicroRNA-7 regulates glioblastoma cell invasion via targeting focal adhesion kinase expression显示文摘背景侵略生长是 glioblastoma 的最典型的生物显型,但是在 glioma 房间侵略的分子的机制是糟糕, understood.Recent 数据证明了 microRNA 在学习试图探索涉及 glioblastoma 房间 invasion.Methods Glioma 房间侵略的控制的 miR-7 的机制的肿瘤 invasion.Our 起一个必要作用被 transwell 评估并且用 miR-7 在 miR-7 的起来规定以后抓试金模仿在 U87 和 U251 cells.Luciferase | WU De-gang WANG Ying-yi FAN Li-gang LUO Hui HAN Bin SUN Li-hua WANG Xie-feng ZHANG Jun-xia CAO Lei WANG Xi-rui YOU Yong-ping LIU Ning | 2011 | Chinese Medical Journal2011，，17： | 23 |
| 6 | The combined genotypes effect of ESR and FSHβ genes on litter size traits in five different pig breeds显示文摘Estrogen receptor (ESR) and Follicular-stimula-ting hormone beta subunit (FSHβ) genes were chosen as candidates to determine whether they control litter size and some other reproductive traits in swine. 269 sows from five different pig breeds were genotyped by an established PCR-RFLPs protocol at both ESR and FSHβ loci. The effects of both ESR and FSHβ on pig reproductive traits, including total number born (TNB) and number born alive (NBA), are analyzed by SAS software (version 6.12). These computation results demonstrated that both ESR locus and FSHβ locus are the major genes influencing litter size in pigs. The sows of BBBB combined genotype of ESR and FSHβ loci generally produce 1.85-3.01 TNB and 2.0-3.0 NBA more than those of ABAA combined genotype. The notable effect of ESR locus and FSHβ locus on litter size of pigs have made it possible to improve the pig reproduction by Marker-assisted selection (MAS). Moreover, introgression of the beneficial alleles into commercial pig breeding lines, | Kefei Chen Ning Li Lusheng Huang Qin Zhang Jiansheng Zhang Shiquan Sun Ming Luo Changxin Wu | 2001 | Chinese Science Bulletin2001，46，2： | 21 |
| 7 | Fibrinogen-like protein 2/fibroleukin prothrombinase contributes to tumor hypercoagulability via IL-2 and IFN-γ显示文摘AIM: To examine the role of Fibrinogen-like protein 2 (fgl2)/fibroleukin in tumor development. Fgl2 has been reported to play a vital role in the pathogenesis in MHV-3 (mouse hepatitis virus) induced fulminant and severe hepatitis,spontaneous abortion,allo-and xeno-graft rejection by mediating 'immune coagulation'. METHODS: Tumor tissues from 133 patients with six types of distinct cancers and the animal tumor tissues from human hepatocellular carcinoma (HCC) model on nude mice (established from high metastasis HCC cell line MHCC97LM6) were obtained. RESULTS: Hfgl2 was detected in tumor tissues from 127 out of 133 patients as well as tumor tissues collected from human HCC nude mice. Hfgl2 was highly expressed both in cancer cells and interstitial inflammatory cells including macrophages,NK cells,and CD8+ T lymphocytes and vascular endothelial cells. Hfgl2 mRNA was localized in cells that expressed hfgl2 protein. Fibrin (nogen) co-localization with hfgl2 expression was determined by dual immunohistochemical staining. In vitro,IL-2 and IFN-γ increased hfgl2 mRNA by 10-100 folds and protein expression in both THP-1 and HUVEC cell lines. One-stage clotting assays demonstrated that THP-1 and HUVEC cells expressing hfgl2 had increased procoagulant activity following cytokines stimulation. CONCLUSION: The hfg12 contributes to the hypercoagulability in cancer and may induce tumor angiogenesis and metastasis via cytokine induction. | Kai Su Fang Chen Wei-Ming Yan Qi-Li Zeng Li Xu Dong Xi Bin Pi Xiao-Ping Luo Qin Ning | 2008 | World Journal of Gastroenterology2008，14，39： | 20 |
| 8 | Effect of Snow-cover Duration on Plant Species Diversity of Alpine Meadows on the Eastern Qinghai-Tibetan Plateau显示文摘Although snow cover plays an important role in structuring plant diversity in the alpine zone,there are few studies on the relationship between snow cover and species diversity of alpine meadows on the eastern Qinghai-Tibetan Plateau.To assess the effect of snow cover on plant species diversity of alpine meadows,we used ten parallel transects of 60m×1m for this study and described the changes in species diversity and composition associated with snow depth.With the division of snow depth into six classes,the highest species richness(S)and species diversity(H')occurred with an intermediate snow depth,i.e.,class III and class IV,showing a unimodal curve with the increase in snow depth.The relationship between snow depth and plant diversity(both richness and Shannon index)could be depicted by quadratic equations.There was no evident relationship between diversity(both S and H')and soil water content,which implied that other more important factors influenced species diversity.The patterns of diversity found in our study were largely attributed to freeze-thaw alteration,length of growing season and disturbances of livestock grazing.Furthermore,snow depth affected species composition,as evaluated by the Srensen's index of similarity.In addition,almost all species limited to one snow depth class were found only in class III and class IV,indicating that intermediate snow depth was suitable for the survival and growth of many alpine species. | CHEN Wennian WU Yan WU Ning LUO Peng | 2008 | Journal of Mountain Science2008，5，4： | 19 |
| 9 | Analysis of Dynamics and Driving Factors of Wetland Landscape in Zoige,Eastern Qinghai-Tibetan Plateau显示文摘Zoige Wetland is one of the largest plateau wetlands in the world.This paper provides a dynamic analysis of spatial and temporal patterns of the wetland in Zoige, Eastern Qinghai-Tibetan Plateau, supported by ERDAS8.7 and ArcGIS9.0.It is the first comparative analysis of a system of rapidly changing wetland with landscape patterns in Zoige, using 3 classified landsat Thematic Mapper images of 1977, 1994 and 2001.The classified images were used to generate wetland distributing maps, and shape index(S), diversity index(H), dominance index(D), evenness index(E), fragmentation index(F) and fractal dimension(Fd) were calculated and analyzed spatiotemporally across pure grazing area in Zoige for each landscape type and in different periods(before 1977, during 1977~1994 and 1994~2001), as well as the driving forces of natural and anthropogenic.The study shows that for a comprehensive understanding of the shapes and trajectories of the shrinking and desertificated land expansion of the wetland, a spatiotemporal landscape metrics analysis in different periods is an improvement than only with landscape changing rates.This type of analysis can also be used to infer underlying social, economic, and political processes that drive the observed wetland forms.The results indicate that wetland patterns can be changed over relatively short periods of time.Thetotal area of lake reduced by 164.86 km2,grassland extended by 141.74 km2,semi-marsh extended by 105.94 km2 , marsh reduced by 86.00 km2;the number of landscape patches reduced by 56, and their average area decreased by 2.68 km2;the successions within lake, marsh, semi-marsh and grassland were found obviously;S decreased stepwise;D and F increased but H decreased;The changing rate after 1994 was 2.3 to 2.9 times greater than that before.The change of the wetland landscape patterns resulted in the interaction between socio-economic and natural forces of positive and negative aspects;and natural factors affected as assistant aspect.Some important human activities in this period led to the change of the landscape patterns in this region directly.Some measurements made by government and NGO delayed the converting process partly. | QIU Pengfei WU Ning LUO Peng WANG Zuyuan LI Maihe | 2009 | Journal of Mountain Science2009，6，1： | 19 |
| 10 | Reversing drug resistance of soft tumor-repopulating cells by tumor cell-derived chemotherapeutic microparticles显示文摘颠倒使人重新住入肿瘤的房间(TRC ) 的药抵抗或起源的发展中的新奇途径像房间的癌症房间是迫切临床的需要改进癌症病人的结果。这里,我们显示出用肿瘤颠倒 TRC 的药抵抗的一条创新途径包含反肿瘤药的导出房间的 microparticles (T-MPs ) 。由比区分的癌症房间更可变形的优点, TRC 优先地收起 T-MPs 在进入房间以后释放反肿瘤药,它接着导致 TRC 的死亡。内在的机制包括防碍药流出并且支持这些药的原子入口。我们的调查结果在用有希望的临床的应用程序颠倒 TRC 的药抵抗在 T-MPs 的举起和一条新奇途径的有效性表明肿瘤房间柔软的重要性。 | Jingwei Ma Yi Zhang Ke Tang Huafeng Zhang Xiaonan Yin Yong Li Pingwei Xu Yanling Sun Ruihua Ma Tiantian Ji Junwei Chen Shuang Zhang Tianzhen Zhang Shunqun Luo Yang Jin Xiuli Luo Chengyin Li Hongwei Gong Zhixiong Long Jinzhi Lu Zhuowei Hu Xuetao Cao Ning Wang Xiangliang Yang Bo Huang | 2016 | Cell Research2016，26，6： | 17 |
| 11 | Spinal CCL2 Promotes Central Sensitization, Long-Term Potentiation, and Inflammatory Pain via CCR2: Further Insights into Molecular, Synaptic, and Cellular Mechanisms显示文摘Mounting evidence supports an important role of chemokines, produced by spinal cord astrocytes, in promoting central sensitization and chronic pain. In particular,CCL2(C-C motif chemokine ligand 2) has been shown to enhance N-methyl-D-aspartate(NMDA)-induced currents in spinal outer lamina Ⅱ(IIo) neurons. However, the exact molecular, synaptic, and cellular mechanisms by which CCL2 modulates central sensitization are still unclear. We found that spinal injection of the CCR2 antagonist RS504393 attenuated CCL2-and inflammation-induced hyperalgesia. Single-cell RT-PCR revealed CCR2 expression in excitatory vesicular glutamate transporter subtype2-positive(VGLUT2^+) neurons. CCL2 increased NMDAinduced currents in CCR2^+/VGLUT2+ neurons in lamina IIo; it also enhanced the synaptic NMD A currents evoked by dorsal root stimulation; and furthermore, it increased the total and synaptic NMDA currents in somatostatinexpressing excitatory neurons. Finally, intrathecal RS504393 reversed the long-term potentiation evoked in the spinal cord by C-fiber stimulation. Our findings suggest that CCL2 directly modulates synaptic plasticity in CCR2-expressing excitatory neurons in spinal lamina IIo, and this underlies the generation of central sensitization in pathological pain. | Rou-Gang Xie Yong-Jing Gao Chul-Kyu Park Ning Lu Ceng Luo Wen-Ting Wang Sheng-Xi Wu Ru-Rong Ji | 2018 | Neuroscience Bulletin2018，34，1： | 17 |
| 12 | Molecular mechanism of indirubin-3'-monoxime and Matrine in the reversal of paclitaxel resistance in NCI-H520/TAX25 cell line显示文摘 | LUO Su-xia DENG Wen-ying WANG Xin-feng LI5 Hui-fang HAN Li-li CHEN Bei-bei CHEN Xiao-bing LI Ning | 2013 | Chinese Medical Journal2013，，5： | 16 |
| 13 | Impact of farmland changes on production potential in China during 1990-2010显示文摘The quantity and spatial pattern of farmland has changed in China, which has led to a major change in the production potential under the influence of the national project of ecological environmental protection and rapid economic growth during 1990–2010. In this study, the production potential in China was calculated based on meteorological, terrain elevation, soil and land-use data from 1990, 2000 and 2010 using the Global Agro-ecological Zones model. Then, changes in the production potential in response to farmland changes from 1990 to 2010 were subsequently analyzed. The main conclusions were the following. First, the total production potential was 1.055 billion tons in China in 2010. Moreover, the average production potential was 7614 kg/ha and showed tremendous heterogeneity in spatial pattern. Total production in eastern China was high, whereas that in northwestern China was low. The regions with high per unit production potential were mainly distributed over southern China and the middle and lower reaches of the Yangtze River. Second, the obvious spatiotemporal heterogeneity in farmland changes from 1990 to 2010 had a significant influence on the production potential in China. The total production potential decreased in southern China and increased in northern China. Furthermore, the center of growth of the production potential moved gradually from northeastern China to northwestern China. The net decrease in the production potential was 2.97 million tons, which occupied 0.29% of the national total actual production in 2010. Third, obvious differences in the production potential in response to farmland changes from 1990 to 2000 and from 2000 to 2010 were detected. The net increase in the production potential during the first decade was 10.11 million tons and mainly distributed in the Northeast China Plain and the arid and semi-arid regions of northern China. The net decrease in the production potential during the next decade was 13.08 million tons and primarily distributed in the middle and lower reaches of the Yangtze River region and the Huang-Huai-Hai Plain. In general, the reason for the increase in the production potential during the past two decades might be due to the reclamation of grasslands, woodlands and unused land, and the reason for the decrease in the production potential might be urbanization that occupied the farmland and Green for Grain Project, which returned farmland to forests and grasslands. | LIU Luo XU Xinliang LIU Jiyuan CHEN Xi NING Jia | 2015 | Journal of Geographical Sciences2015，25，1： | 16 |
| 14 | Distributed processing based fault location,isolation,and service restoration method for active distribution network显示文摘Active distribution network(ADN)is a solution for power system with interconnection of distributed energy resources(DER),which may change the network operation and power flow of traditional power distribution network.However,in some circumstances the malfunction of protection and feeder automation in distribution network occurs due to the uncertain bidirectional power flow.Therefore,a novel method of fault location,isolation,and service restoration(FLISR)for ADN based on distributed processing is proposed in this paper.The differential-activated algorithm based on synchronous sampling for feeder fault location and isolation is studied,and a framework of fault restoration is established for ADN.Finally,the effectiveness of the proposed algorithm is verified via computer simulation of a case study for active distributed power system. | Jiaming WENG Dong LIU Ning LUO Xueyong TANG | 2015 | Journal of Modern Power Systems and Clean Energy2015，3，4： | 16 |
| 15 | Prevalence of vitamin D deficiency in girls with idiopathic central precocious puberty显示文摘在维生素 D 缺乏和自发的中央早熟的发身(ICPP ) 之间的关系最近被记录了。在这研究, 280 个女孩与 ICPP 诊断了,类似的年龄的 188 个正常发身控制女孩被注册并且回顾地学习了。有的 ICPP 组显著地降低浆液 25-hydroxyvitamin D (25 [哦] D ) 层次比控制组。而且,一种非线性的关系在浆液 25 之间被发现[哦] D 和 ICPP,和为浆液 25 的一个截止的点[哦] D 有或没有调整不同惊讶因素为 ICPP 在 31.8 ng/ml 被发现。有浆液 25 的女孩[哦] D31.8 ng/ml 有更低的机会比率(unadjusted:或 0.36, 95% CI 0.15 到 0.83, P < 0.05;调整的高度和重量:或 0.44, 95% CI 0.18 到 1.08, P = 0.072;BMI 调整了:或 0.36, 95% CI 0.16 到 0.84, P < 0.05 ) 。有 25 的 ICPP 题目[哦] D 缺乏从二另外的亚群比这些题目有一个更高的身体团索引(BMI ) 。关联分析证明那维生素 D 水平在 ICPP 组与 BMI 和一些新陈代谢的参数被相关。我们的学习建议那维生素 D 地位可以与 ICPP 风险被联系并且可以在 ICPP 上有阀值效果。 | Yue Zhao Wenjun Long Caiqi Du Huanhuan Yang Shimin Wu Qin Ning Xiaoping Luo | 2018 | Frontiers of Medicine2018，12，2： | 16 |
| 16 | Serum hepatitis B virus RNA is a predictor of HBeAg seroconversion and virological response with entecavir treatment in chronic hepatitis B patients显示文摘BACKGROUND Characteristics of alterations of serum hepatitis B virus(HBV) RNA in different chronic hepatitis B(CHB) patients still cannot be fully explained. Whether HBV RNA can predict HBeAg seroconversion is still controversial.AIM To investigate whether HBV RNA can predict virological response or HBeAg seroconversion during entecavir(ETV) treatment when HBV DNA is undetectable.METHODS The present study evaluated 61 individuals who were diagnosed and treated with long-term ETV monotherapy at the Department of Infectious Diseases of Peking University First Hospital(China) from September 2006 to December 2007.Finally, 30 treatment-naive individuals were included. Serum HBV RNA were extracted from 140 μL serum samples at two time points. Then they were reverse transcribed to cDNA with the HBV-specific primer. The product was quantified by real-time quantitative PCR(RT-PCR) using TAMARA probes. Statistical analyses were performed with IBM SPSS 20.0.RESULTS Level of serum HBV RNA at baseline was 4.15 ± 0.90 log10 copies/mL. HBV RNA levels showed no significant difference between the virological response(VR)and partial VR(PVR) groups at baseline(P = 0.940). Serum HBV RNA significantly decreased among patients who achieved a VR during ETV therapy(P < 0.001). The levels of HBV RNA in both HBeAg-positive patients with seroconversion group and those with no seroconversion increased after 24 wk of treatment. Overall, HBV RNA significantly but mildly correlated to HBsAg(r =0.265, P = 0.041), and HBV RNA was not correlated to HBV DNA(r = 0.242, P =0.062). Furthermore, serum HBV RNA was an independent indicator for predicting HBeAg seroconversion and virological response. HBeAg seroconversion was more likely in CHB patients with HBV RNA levels below4.12 log10 copies/mL before treatment.CONLUSION The level of serum HBV RNA could predict HBeAg seroconversion and PVR during treatment. In the PVR group, the level of serum HBV RNA tends to be increasing. | Hao Luo Xia-Xia Zhang Li-Hua Cao Ning Tan Qian Kang Hong-Li Xi Min Yu Xiao-Yuan Xu | 2019 | World Journal of Gastroenterology2019，25，6： | 15 |
| 17 | Clinical significance of HOTAIR expression in colon cancer显示文摘AIM: To detect the expression of the long noncoding RNA HOTAIR in colon cancer and analyze its relationship with clinicopathological parameters of colon cancer. METHODS: Total RNA was extracted from 80 colon cancer tissues and matched tumor-adjacent normal colon tissues and reverse transcribed. Quantitative polymerase chain reaction was used to detect the expression of HOTAIR. The relationship between the expression of HOTAIR and clinicopathological parameters of colon cancer was analyzed. RESULTS: The expression of HOTAIR was significantly higher in colon cancer tissues than in matched tumoradjacent normal colon tissues(P < 0.05). HOTAIR expression was significantly higher in cases with lymph node metastasis than in those without metastasis; in lowly differentiated and undifferentiated cases than in highly and moderately differentiated cases; and in stages Ⅲ + Ⅳ cases than in stages?Ⅰ?+ Ⅱ cases(P < 0.05).CONCLUSION: HOTAIR expression is upregulated in colon cancer, suggesting that HOTAIR plays an important role in the tumorigenesis, development and metastasis of colon cancer. HOTAIR may act as an oncogene and represents a new molecular target for the treatment of colon cancer. | Zhi-Fen Luo Dan Zhao Xi-Qing Li Yong-Xia Cui Ning Ma Chuang-Xin Lu Ming-Yue Liu Yun Zhou | 2016 | World Journal of Gastroenterology2016，22，22： | 14 |
| 18 | Staged carotid artery angioplasty and stenting for patients with high-grade carotid stenosis with high risk of developing hyperperfusion injury:a retrospective analysis of 44 cases显示文摘Background:Hyperperfusion syndrome(HPS)is a rare but potentially a life-threatening complication after carotid artery angioplasty and stenting(CAS).Staged CAS has been an alternative to prevent HPS.Materials and methods:44 of 908 patients with high-grade internal carotid artery stenosis or near occlusion were at risk of HPS because of poor collateral flow and impaired cerebral blood flow(CBF).They were treated with first(stage 1),followed by a full CAS(stage 2)1 month later.Their 30-day outcomes were tabulated and analysed.Results:During follow-up,1 of the 44(2.2%)patients developed HPS immediately,3(7%)had postprocedural HPS(ie,transcranial Doppler(TCD)>120%)without clinical symptoms and 3(7%)required stenting at stage 1 for carotid dissections.After stage 1,there were significant improvement between the preprocedural and postprocedural CBF(0.98±0.06 vs 0.85±0.05,p<0.05),mean transit time(MTT;1.05±0.05 vs 1.15±0.05,p<0.05),time to peak(TTP;1.04±0.06 vs 1.20±0.06,p<0.05)on CT perfusion(CTP),and CBF(66.41±7.41 vs 44.44±6.43,p<0.05)on TCD.After stage 2,improvement was seen in CBF(1.01±0.07 vs 0.98±0.06,p<0.05),MTT(1.01±0.05 vs 1.05±0.05,p<0.05),TTP(0.99±0.06 vs 1.04±0.06,p<0.05)on CTP and CBF(66.41±7.41 vs 93.78±18.81,p<0.05)on TCD.2 had postoperative increase of middle cerebral artery mean flow velocity of 120%after stage 2 without clinical symptoms.Conclusion:Staged carotid artery stenting probably decreased the chance of developing HPS in this group of selected patients.Although requiring a 2-step intervention,staged CAS may be a safe and effective alternative. | Dapeng Mo Gang Luo Bo Wang Ning Ma Feng Gao Xuan Sun Xiaotong Xu Zhongrong Miao | 2016 | Stroke & Vascular Neurology2016，1，4： | 14 |
| 19 | Hypoxia-inducible factor-1α induces the epithelial-mesenchymal transition of human prostatecancer cells显示文摘Background Hypoxia-inducible factor-1α (HIF-1α) is a transcriptional factor that could improve the stimulation of angiogenesis and the metabolic adaptation of tumor cells to hypoxia. A recent study showed that HIF-1α could induce colon cancer cells epithelial-mesenchymal transition (EMT). However, no evidence indicates a similar correlation in human prostate cancer cells. This study was designed to evaluate the effect of HIF-1α over-expression on the EMT in human prostate cancer cells. Methods We selected the appropriate cell line for HIF-1α induction from those EMT negative prostate cell lines through vimentin gene detection by RT-PCR. As the result, LNCaP cell line is the best one for further experiment. LNCaP cells were transfected with recombinant plasmid pcDNA3.1(-)/HIF-1α and pcDNA3.1(-) control vector by Lipofectamine 2000 system. The positive cell colonies were confirmed by indirect immunofluorescence labeling. Then Transwell polycarbonate filter was used to analyze the invasive potency. The expression of EMT associated proteins, E-cadherin and vimentin, was detected by Western blotting. Results Among four of the EMT negative cell lines, LNCaP was the only one expressed the vimentin gene but not the associated protein. The expression level of HIF-1α in LNCaP/HIF-1α was distinctly higher than that in LNCaP/pcDNA3.1 and LNCaP. The cell numbers of LNCaP/HIF-1α that penetrated through the Transwell filter were higher than that of LNCaP/pcDNA3.1 and LNCaP. Compared with the LNCaP/pcDNA3.1 and LNCaP cells, the expression of vimentin was up-regulated in LNCaP/HIF-1α, whereas the expression of E-cadherin was down-regulated. Conclusions Over-expression of HIF-1α stimulates the invasion potency of human prostate carcinoma cells through EMT pathway. The expression of E-cadherin and vimentin, playing established roles in EMT, could be regulated by HIF-1α in human prostate cancer cell line. | LUO Yong HE Da-lin NING Liang SHEN Shu-lin LI Lei LI Xiang | 2006 | Chinese Medical Journal2006，，9： | 13 |
| 20 | Meningeal lymphatic vessels regulate brain tumor drainage and immunity显示文摘Recent studies have shown that meningeal lymphatic vessels(MLVs),which are located both dorsally and basally beneath the skull,provide a route for draining macromolecules and trafficking immune cells from the central nervous system(CNS)into cervical lymph nodes(CLNs),and thus represent a potential therapeutic target for treating neurodegenerative and neuroinflammatory diseases.However,the roles of MLVs in brain tumor drainage and immunity remain unexplored.Here we show that dorsal MLVs undergo extensive remodeling in mice with intracranial gliomas or metastatic melanomas.RNA-seq analysis of MLV endothelial cells revealed changes in the gene sets involved in lymphatic remodeling,fluid drainage,as well as inflammatory and immunological responses.Disruption of dorsal MLVs alone impaired intratumor fluid drainage and the dissemination of brain tumor cells to deep CLNs(dCLNs).Notably,the dendritic cell(DC)trafficking from intracranial tumor tissues to dCLNs decreased in mice with defective dorsal MLVs,and increased in mice with enhanced dorsal meningeal lymphangiogenesis.Strikingly,disruption of dorsal MLVs alone,without affecting basal MLVs or nasal LVs,significantly reduced the efficacy of combined anti-PD-1/CTLA-4 checkpoint therapy in striatal tumor models.Furthermore,mice bearing tumors overexpressing VEGF-C displayed a better response to anti-PD-1/CTLA-4 combination therapy,and this was abolished by CCL21/CCR7 blockade,suggesting that VEGF-C potentiates checkpoint therapy via the CCL21/CCR7 pathway.Together,the results of our study not only demonstrate the functional aspects of MLVs as classic lymphatic vasculature,but also highlight that they are essential in generating an efficient immune response against brain tumors. | Xueting Hu Qiuping Deng Lu Ma Qingqing Li Yidong Chen Yuhan Liao Fan Zhou Chen Zhang Linlin Shao Jun Feng Tubao He Weihai Ning Yan Kong Yingqing Huo Aibin He Bing Liu Jingjing Zhang Ralf Adams Yulong He Fuchou Tang Xiuwu Bian Jincai Luo | 2020 | Cell Research2020，30，3： | 11 |
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