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| 1 | Erianin,a novel dibenzyl compound in Dendrobium extract,inhibits lung cancer cell growth and migration via calcium/calmodulin-dependent ferroptosis显示文摘Ferroptosis,a novel form of programmed cell death,is characterized by iron-dependent lipid peroxidation and has been shown to be involved in multiple diseases,including cancer.Stimulating ferroptosis in cancer cells may be a potential strategy for cancer therapy.Therefore,ferroptosis-inducing drugs are attracting more attention for cancer treatment.Here,we showed that erianin,a natural product isolated from Dendrobium chrysotoxum Lindl,exerted its anticancer activity by inducing cell death and inhibiting cell migration in lung cancer cells.Subsequently,we demonstrated for the first time that erianin induced ferroptotic cell death in lung cancer cells,which was accompanied by ROS accumulation,lipid peroxidation,and GSH depletion.The ferroptosis inhibitors Fer-1 and Lip-1 but not Z-VAD-FMK,CQ,or necrostatin-1 rescued erianin-induced cell death,indicating that ferroptosis contributed to erianin-induced cell death.Furthermore,we demonstrated that Ca^(2+)/CaM signaling was a critical mediator of erianin-induced ferroptosis and that blockade of this signaling significantly rescued cell death induced by erianin treatment by suppressing ferroptosis.Taken together,our data suggest that the natural product erianin exerts its anticancer effects by inducing Ca^(2+)/CaMdependent ferroptosis and inhibiting cell migration,and erianin will hopefully serve as a prospective compound for lung cancer treatment. | Peng Chen Qibiao Wu Jiao Feng Lili Yan Yitian Sun Shuiping Liu Yu Xiang Mingming Zhang Ting Pan Xiaying Chen Ting Duan Lijuan Zhai Bingtao Zhai Wengang Wang Ruonan Zhang Bi Chen Xuemeng Han Yicong Li Liuxi Chen Ying Liu Xingxing Huang Ting Jin Wenzheng Zhang Hong Luo Xiaohui Chen Yongqiang Li Qiujie Li Guohua Li Qin Zhang Lvjia Zhuo Zuyi Yang Huifen Tang Tian Xie Xiaoping Ouyang Xinbing Sui | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 42 |
| 2 | 5-Hydroxymethylcytosine signatures in circulating cell-free DNA as diagnostic biomarkers for human cancers显示文摘象 5-methylcytosine (5mC ) 和 5-hydroxymethylcytosine (5hmC ) 那样的 DNA 修正是知道在哺乳动物影响全球基因表示的 epigenetic 标记。在人的染色体,与基因表示的靠近的关联和高化学的稳定性给他们的流行,这些 DNA epigenetic 标记能为癌症用作理想的 biomarkers 诊断。利用一种高度敏感、选择的化学标记技术,我们这里报导在传播没有房间的 DNA ( cfDNA )并且在从最近与 colorectal 诊断的 260 个病人的一个队收集的配对的肿瘤和邻近的纸巾的 genomic DNA ( gDNA )的 5hmC 的染色体宽的介绍,胃,胰腺,从 90 个健康个人的肝或甲状腺癌症和正常纸巾。5hmC 主要在与开的染色质和容许的 histone 修正重合的 transcriptionally 活跃的区域被散布。柔韧的联系癌症的 5hmC 签名在 cfDNA 被识别为特定的癌症类型是特征的。传播 cfDNA 的 5hmC 底 biomarkers colorectal 和胃的癌症是高度预兆的并且比常规 biomarkers 优异并且比得上从织物活体检视的 5hmC biomarkers。因此,这新策略能从血样品的分析为癌症的诊断和预后导致有效、最低限度地侵略的方法的发展。 | Wenshuai Li Xu Zhang Xingyu Lu Lei You Yanqun Song Zhongguang Luo Jun Zhang Ji Nie Wanwei Zheng Diannan Xu Yaping Wang Yuanqiang Dong Shulin Yu Jun Hong Jianping Shi Hankun Hao Fen Luo Luchun Hua Peng Wang Xiaoping Qian Fang Yuan Lianhuan Wei Ming Cui Taiping Zhang Quan Liao Menghua Dai Ziwen Liu Ge Chen Katherine Meckel Sarbani Adhikari Guifang Jia Marc B Bissonnette Xinxiang Zhang Yupei Zhao Wei Zhang Chuan He Jie Liu | 2017 | Cell Research2017，27，10： | 30 |
| 3 | TRPV1 activation improves exercise endurance and energy metabolism through PGC-1α upregulation in mice显示文摘 | Zhidan Luo Liqun Ma Zhigang Zhao Hongbo He Dachun Yang Xiaoli Feng Shuangtao Ma Xiaoping Chen Tianqi Zhu Tingbing Cao Daoyan Liu Bernd Nilius Yu Huang Zhencheng Yan Zhiming Zhu | 2012 | Cell Research2012，22，3： | 18 |
| 4 | Prevalence of vitamin D deficiency in girls with idiopathic central precocious puberty显示文摘在维生素 D 缺乏和自发的中央早熟的发身(ICPP ) 之间的关系最近被记录了。在这研究, 280 个女孩与 ICPP 诊断了,类似的年龄的 188 个正常发身控制女孩被注册并且回顾地学习了。有的 ICPP 组显著地降低浆液 25-hydroxyvitamin D (25 [哦] D ) 层次比控制组。而且,一种非线性的关系在浆液 25 之间被发现[哦] D 和 ICPP,和为浆液 25 的一个截止的点[哦] D 有或没有调整不同惊讶因素为 ICPP 在 31.8 ng/ml 被发现。有浆液 25 的女孩[哦] D31.8 ng/ml 有更低的机会比率(unadjusted:或 0.36, 95% CI 0.15 到 0.83, P < 0.05;调整的高度和重量:或 0.44, 95% CI 0.18 到 1.08, P = 0.072;BMI 调整了:或 0.36, 95% CI 0.16 到 0.84, P < 0.05 ) 。有 25 的 ICPP 题目[哦] D 缺乏从二另外的亚群比这些题目有一个更高的身体团索引(BMI ) 。关联分析证明那维生素 D 水平在 ICPP 组与 BMI 和一些新陈代谢的参数被相关。我们的学习建议那维生素 D 地位可以与 ICPP 风险被联系并且可以在 ICPP 上有阀值效果。 | Yue Zhao Wenjun Long Caiqi Du Huanhuan Yang Shimin Wu Qin Ning Xiaoping Luo | 2018 | Frontiers of Medicine2018，12，2： | 16 |
| 5 | Carbon pools and fluxes in the China Seas and adjacent oceans显示文摘The China Seas include the South China Sea, East China Sea, Yellow Sea, and Bohai Sea. Located off the Northwestern Pacific margin, covering 4700000 km^2 from tropical to northern temperate zones, and including a variety of continental margins/basins and depths, the China Seas provide typical cases for carbon budget studies. The South China Sea being a deep basin and part of the Western Pacific Warm Pool is characterized by oceanic features; the East China Sea with a wide continental shelf, enormous terrestrial discharges and open margins to the West Pacific, is featured by strong cross-shelf materials transport; the Yellow Sea is featured by the confluence of cold and warm waters; and the Bohai Sea is a shallow semiclosed gulf with strong impacts of human activities. Three large rivers, the Yangtze River, Yellow River, and Pearl River, flow into the East China Sea, the Bohai Sea, and the South China Sea, respectively. The Kuroshio Current at the outer margin of the Chinese continental shelf is one of the two major western boundary currents of the world oceans and its strength and position directly affect the regional climate of China. These characteristics make the China Seas a typical case of marginal seas to study carbon storage and fluxes. This paper systematically analyzes the literature data on the carbon pools and fluxes of the Bohai Sea,Yellow Sea, East China Sea, and South China Sea, including different interfaces(land-sea, sea-air, sediment-water, and marginal sea-open ocean) and different ecosystems(mangroves, wetland, seagrass beds, macroalgae mariculture, coral reefs, euphotic zones, and water column). Among the four seas, the Bohai Sea and South China Sea are acting as CO_2 sources, releasing about0.22 and 13.86–33.60 Tg C yr^(-1) into the atmosphere, respectively, whereas the Yellow Sea and East China Sea are acting as carbon sinks, absorbing about 1.15 and 6.92–23.30 Tg C yr^(-1) of atmospheric CO_2, respectively. Overall, if only the CO_2 exchange at the sea-air interface is considered, the Chinese marginal seas appear to be a source of atmospheric CO_2, with a net release of 6.01–9.33 Tg C yr^(-1), mainly from the inputs of rivers and adjacent oceans. The riverine dissolved inorganic carbon (DIC) input into the Bohai Sea and Yellow Sea, East China Sea, and South China Sea are 5.04, 14.60, and 40.14 Tg C yr^(-1),respectively. The DIC input from adjacent oceans is as high as 144.81 Tg C yr^(-1), significantly exceeding the carbon released from the seas to the atmosphere. In terms of output, the depositional fluxes of organic carbon in the Bohai Sea, Yellow Sea, East China Sea, and South China Sea are 2.00, 3.60, 7.40, and 5.92 Tg C yr^(-1), respectively. The fluxes of organic carbon from the East China Sea and South China Sea to the adjacent oceans are 15.25–36.70 and 43.93 Tg C yr^(-1), respectively. The annual carbon storage of mangroves, wetlands, and seagrass in Chinese coastal waters is 0.36–1.75 Tg C yr^(-1), with a dissolved organic carbon(DOC) output from seagrass beds of up to 0.59 Tg C yr^(-1). Removable organic carbon flux by Chinese macroalgae mariculture account for 0.68 Tg C yr^(-1) and the associated POC depositional and DOC releasing fluxes are 0.14 and 0.82 Tg C yr^(-1), respectively. Thus, in total, the annual output of organic carbon, which is mainly DOC, in the China Seas is 81.72–104.56 Tg C yr^(-1). The DOC efflux from the East China Sea to the adjacent oceans is 15.00–35.00 Tg C yr^(-1). The DOC efflux from the South China Sea is 31.39 Tg C yr^(-1). Although the marginal China Seas seem to be a source of atmospheric CO_2 based on the CO_2 flux at the sea-air interface, the combined effects of the riverine input in the area, oceanic input, depositional export,and microbial carbon pump(DOC conversion and output) indicate that the China Seas represent an important carbon storage area. | Nianzhi JIAO Yantao LIANG Yongyu ZHANG Jihua LIU Yao ZHANG Rui ZHANG Meixun ZHAO Minhan DAI Weidong ZHAI Kunshan GAO Jinming SONG Dongliang YUAN Chao LI Guanghui LIN Xiaoping HUANG Hongqiang YAN Limin HU Zenghu ZHANG Long WANG Chunjie CAO Yawei LUO Tingwei LUO Nannan WANG Hongyue DANG Dongxiao WANG Si ZHANG | 2018 | Science China Earth Sciences2018，61，11： | 12 |
| 6 | Experimental and numerical study on a micro jet cooling solution for high power LEDs显示文摘An active cooling solution based on close-looped micro impinging jet is proposed for high power light emitting diodes (LEDs). In this system, a micro pump is utilized to enable the fluid circulation, impinging jet is used for heat exchange between LED chips and the present system. To check the feasibility of the present cooling system, the preliminary experiments are conducted without the intention of parameter opti-mization on micro jet device and other system components. The experiment results demonstrate that the present cooling system can achieve good cooling effect. For a 16.4 W input power, the surface temperature of 2 by 2 LED array is just 44.2℃ after 10 min operation, much lower than 112.2℃, which is measured without any active cool-ing techniques at the same input power. Experimental results also show that increase in the flow rate of micro pump will greatly enhance the heat transfer efficiency, how-ever, it will increase power consumption. Therefore, it should have a trade-off be-tween the flow rate and the power consumption. To find a suitable numerical model for next step parameter optimization, numerical simulation on the above experiment system is also conducted in this paper. The comparison between numerical and ex-periment results is presented. For two by two chip array, when the input power is 4 W, the surface average temperature achieved by a steady numerical simulation is 34℃, which is close to the value of 32.8℃ obtained by surface experiment test. The simu-lation results also demonstrate that the micro jet device in the present cooling sys-tem needs parameter optimization. | LUO XiaoBing LIU Sheng JIANG XiaoPing CHENG Ting | 2007 | Science China(Technological Sciences)2007，50，4： | 12 |
| 7 | Meditation promotes insightful problem-solving by keeping people in amindful and alert conscious state显示文摘Although previous studies have shown that sleep can inspire insight,it is still unclear whether meditation can promote insight.Meditation differs from other types of passive rest such as relaxation and sleep because it requires full consciousness andmindfulness of targets such as one's breathing.Forty-eight university students without meditation experience were recruited to learn a simple meditation technique.They were given a list of 10 insight problems to solve (the pre-test session).In this study,we focused on the unsolved problems and examined if they could be successfully solved after a 20min rest interval with or without meditation.Results showed that relative to the control group that listened to Chinese or English words and made a language judgment,the groups who learned meditation successfully solved significantly more failed problems from the pre-test session,providing direct evidence for the role of meditation in promoting insight.Further analysis showed that maintaining amindful and alert state during meditation (raising a hand to report every 10 deep breaths compared to every 100 deep breaths) resulted in more insight regarding the failed items from the pre-test session.This implies that it was watchfulness in meditation,rather than relaxation,that actually contributed to insight.Consistently,in the meditation session or control task,the percentage of alpha waves—a brain index of mental relaxation—was negatively correlated with insight.These results suggest a meditation-based insight-promoting mechanism different from that involved in passive rest such as relaxation and sleep. | REN Jun HUANG ZhiHui LUO Jing WEI GaoXia YING XiaoPing DING ZhiGuang WU YiBin LUO Fei | 2011 | Science China(Life Sciences)2011，54，10： | 11 |
| 8 | An antiretroviral regimen containing 6 months of stavudine followed by long-term zidovudine for first-fine HIV therapy is optimal in resource-limited settings: a prospective, multicenter study in China显示文摘 | Li Taisheng Guo Fuping Li Yijia Zhang Chengda Han Yang Lye Wei He Yun Lu Hongzhou Xie Jing Huang Aiqiong Li Yanling Tang Xiaoping Wang Hui Zhang Tong Gao Guiju Lei Junkang Zhang Xiaoying Wu Xinhua Sun Yongtao Bai Jinsong Luo Ling Wang Huanlmg | 2014 | Chinese Medical Journal2014，，1： | 6 |
| 9 | New Record of Palaeoscolecids from the Early Cambrian of Yunnan,China显示文摘摘要:新 palaeoscolecid, Guanduscolex 次要的胡,罗 et Fu 情报。et sp。nov,与内向的人和肠的保存软部分来自云南的更低的寒武纪的 Guanshan 动物志,华南。扫描电子显微镜学(SEM ) 揭示的表皮的微结构细节证明每个环忍受三排盘子,每个盘子忍受 910 边缘并且 45 个中央节点。这发现在关系和 palaeoscolecids 和另外的早 priapulids 的进化小径上打开新灯。 | HU Shixue LI Yong LUO Huilin FU Xiaoping YOU Ting PANG Jiyuan LIU Qi Michael STEINER | 2008 | Acta Geologica Sinica(English Edition)2008，82，2： | 6 |
| 10 | Analysis on Dam-Breaking Mode of Tangjiashan Barrier Dam in Beichuan County显示文摘Tangjiashan landslide is a typical high-speed consequent landslide of medium-steep dip angle. This landslide triggered by earthquake took place in about semi-minute. The relative sliding displacement is 900 meters, so average sliding speed is about 30 meters per second. The longitudinal length of barrier dam which is formed by high-speed landslide along river is 803.4 meters; and maximum width crossing river is 611.8 meters. And its volume is estimated about 20.37 million steres. Through detailed geological investigation of the barrier dam, together with early geological information before earthquake, geological structures of the barrier dam and its stability of upstream and downstream slopes are studied when water level reaches different elevations in condition of continual after shocks with seismic intensity of 7 or 8 Richter scale. On this basis, dam-breaking mode of barrier dam is discussed deeply. Thereby, analytic results provide significant guidance and advices to front headquarters of Tangjiashan barrier dam, so that some proper engineering measures can be implemented and flood discharge can be carried out well. | HU Xiewen LUO Gang LV Xiaoping HUANG Runqiu SHI Yubin | 2011 | Journal of Mountain Science2011，8，2： | 6 |
| 11 | The efficacy and safety of nimodipine in acute ischemic stroke patients with mild cognitive impairment: a double-blind, randomized,placebo-controlled trial显示文摘Nimodipine might be effective in subcortical vascular dementia(VaD). Its benefit in preventing further cognitive decline in patients with acute ischemic stroke(AIS) and vascular mild cognitive impairment(VaMCI) remains to be established. In this multicenter, double-blind trial, we randomly assigned 654 eligible patients to nimodipine 30 mg three times a day or placebo. The primary outcome was any cognitive decline defined by the changes on the Mini-Mental State Examination(DMMSE à3) or vascular AD assessment scale cognitive subscale(DADAS-cog ! 4) at 6 months. Secondary outcomes included any distribution shift of DADAS-cog, DMMSE or cognitive improvement defined by DADAS-cog à2, or DMMSE ! 0. The primary outcome in the nimodipine group and placebo group were similar for DMMSE à3(4.18% and 7.22%, respectively, P = 0.15) and DADAS-cog ! 4(8.36% and 8.93% respectively,P = 0.88). The distribution shift of DADAS-cog and DMMSE differed significantly between the two groups(P = 0.03 and P = 0.05 respectively). Cognitive improvement occurred in 55.4% in the nimodipine group and 43.6% in the placebo group measured by DADAS-cog à2(Odds Ratio, 1.54; 95% confidence interval[CI] 1.10–2.14, P < 0.01) or 84.0% and 74.6% respectively by DMMSE ! 0(Odds Ratio, 1.79; 95% CI 1.18–2.70, P < 0.01). Nimodipine was associated with better cognitive function in the memory domain. The adverse events rate was similar in two groups. This study is registered with ClinicalTrials.gov,NCT01220622. Nimodipine did not show benefit to prevent cognitive decline in AIS patients with VaMCI, but improved cognition moderately, especially measured in the memory domain. | Huaguang Zheng Yilong Wang Anxin Wang Hao Li David Wang Xingquan Zhao Penglian Wang Haipeng Shen Lijun Zuo Yuesong Pan Zixiao Li Xia Meng Xianwei Wang Weixiong Shi Yi Ju Liping Liu Kehui Dong Chunxue Wang Rubo Sui Rong Xue Xiaoping Pan Xiaoyua Niu Benyan Luo Yi Sui Huali Wang Tao Feng Yongjun Wang | 2019 | Science Bulletin2019，64，2： | 5 |
| 12 | Transgene directionally integrated into C-genome of Brassica napus显示文摘Integration of a transgene into a C-genome chromosome plays an important role in reducing ecological risk of transgenic Brassica napus To obtain C-genome transgenic B. napus, herbi- cide-resistant bar gene was firstly transferred into B. oleracea var. alboglabra mediated by Agrobacterium tumefaciens strain LBA4404. Then using the trans- genic B. oleracea as paternal plants and 8 non- transgenic varieties of B. rapa as maternal plants, C- genome transgenic B. napus with bar gene was arti- ficially resynthesized by means of ovary culture and chromosome doubling. Among 67 lines of the resyn- thesized B. napus, 31 were positive, and 36 were negative according to PCR test for bar gene. At least 2 plants from each line were kept for PPT spray con- firmation. The result was in consistence with the PCR test. Genomic Southern blotting of three randomly chosen lines also showed that bar gene had been integrated into the genome of resynthesized B. napus lines. | LI Jun FANG Xiaoping WANG Zhuan LUO Lixia HU Qiong | 2006 | Chinese Science Bulletin2006，51，13： | 5 |
| 13 | A New Arthropod,Guangweicaris Luo,Fu et Hu gen. nov. from the Early Cambrian Guanshan Fauna,Kunming,China显示文摘The Guanshan Fauna is a soft-bodied fauna dominated by arthropods (including trilobites, trilobitoides, Tuzoia, Isoxys, and bradorids) in association with priapulids, brachiopods, anomalocaridids, vetulicoliids, sponges, chancellorids, and echinoderms. This paper reports and describes a new arthropod from the yellowish green mudstone at the lower part of the Wulongqing Formation, Canglangpuan Stage, Lower Cambrian in Kunming, Yunnan, China. The stratigraphic and geographic distribution, classification, fossil preservation, life style of this new arthropod and comparisons with other fossil arthropods are also discussed in details. The discovery and research of the non-mineralized arthropod, Guangweicaris Luo, Fu et Hu gen. nov. from the Guanshan Fauna adds new members to the taxonomic list and provides new information to the evolution of early arthropods. Furthermore, this study would shed new light into the “Cambrian Explosion” and the evolution of early life. | LUO Huilin FU Xiaoping HU Shixue LI Yong HOU Shuguang YOU Ting PANG Jiyuan LIU Qi | 2007 | Acta Geologica Sinica(English Edition)2007，81，1： | 5 |
| 14 | Influence of nanoparticle concentrations on flow boiling heat transfer coefficients of Al_2O_3/R141b in micro heat exchanger by direct metal laser sintering显示文摘Al_2O_3/R141 b + Span-80 nanorefrigerant for 0.05 wt.% to 0.4 wt.% is prepared by ultrasonic vibration to investigate the influence of nanoparticle concentrations on flow boiling heat transfer of Al_2O_3/R141 b + Span-80 in micro heat exchanger by direct metal laser sintering. Experimental results show that nanoparticle concentrations have significantly impact on heat transfer coefficients by homogeneity test of variances according to mathematical statistics. The heat transfer performance of Al_2O_3/R141 b+ Span-80 nanorefrigerant is enhanced after adding nanoparticles in the pure refrigerant R141 b. The heat transfer coefficients of 0.05 wt.%, 0.1 wt.%, 0.2 wt.%, 0.3 wt.%and 0.4 wt.% Al_2O_3/R141 b + Span-80 nanorefrigerant respectively increase by 55.0%, 72.0%, 53.0%, 42.3% and 39.9% compared with the pure refrigerant R141 b. The particle fluxes from viscosity gradient, non-uniform shear rate and Brownian motion cause particles to migrate in fluid especially in the process of flow boiling. This migration motion enhances heat transfer between nanoparticles and fluid. Therefore, the heat transfer performance of nanofluid is enhanced. It is important to note that the heat transfer coefficients nonlinearly increase with nanoparticle concentrations increasing. The heat transfer coefficients reach its maximum value at the mass concentration of 0.1% and then it decreases slightly. There exists an optimal mass concentration corresponding to the best heat transfer enhancement. The reason for the above phenomenon is attributed to nanoparticles deposition on the minichannel wall by Scanning Electron Microscopy observation. The channel surface wettability increases during the flow boiling experiment in the mass concentration range from 0.2 wt.% to 0.4 wt.%. The channel surface with wettability increasing needs more energy to produce a bubble. Therefore, the heat transfer coefficients decrease with nanoparticle concentrations in the range from 0.2 wt.% to 0.4 wt.%. In addition, a new correlation has been proposed by fitting the experimental data considering the influence of mass concentrations on the heat transfer performance. The new correlation can effectively predict the heat transfer coefficient. | Jianyang Zhou Xiaoping Luo Cong Deng Mingyu Xie Lin Zhang Di Wu Feng Guo | 2017 | Chinese Journal of Chemical Engineering2017，25，12： | 4 |
| 15 | Ultrastructure of focal cerebral cortex tissue from rats with focal cortical dysplasia显示文摘BACKGROUND: Developing a model of focal cortical dysplasia in microgyrus and observing the ultrastructure of focal tissue is of important significance for analyzing the pathology of cortical developmental disorder and the factors of structural changes. OBJECTIVE: This study was to observe the pathological characteristics of focal tissue around the microgyrus of rats with cortical developmental disorder using an electron microscope, so as to analyze the causes associated with cerebral cortical developmental disorder. DESIGN: A randomized controlled animal experiment. SETTING: The First Affiliated Hospital of Chongqing Medical University. MATERIALS: This study was carried out in the Chongqing Key Laboratory of Neurology, Room for Electron Microscope of Chongqing Medical University, and Laboratory Animal Center, Research Institute of Surgery, Daping Hospital, Third Military Medical University of Chinese PLA between January 2004 and August 2006. Eighteen healthy newborn male Wistar rats, weighing 3.0-6.0 g, provided by the Laboratory Animal Center, Daping Hospital, Third Military Medical University of Chinese PLA, were involved in this study. The protocol was carried out in accordance with animal ethics guidelines for the use and care of animals. Probes (Chongqing Wire & Cable Factory, China) were made of copper core wire with diameter of 1 mm. METHODS: The rats were randomly divided into 3 groups with 6 in each: normal control group, liquid nitrogen injured group and sham-operation group. ①In the liquid nitrogen injured group, a blunt probe frozen by liquid nitrogen was placed on fronto-parietal crinial bone of rats for 8 s. A 3-5 cm of microgyrus was induced in the unilateral cerebral sensory cortical area. In the sham-operation group, probe was placed at the room temperature. In the normal control group, rats were untouched. ② The conscious state and electrical activity of brain of rats in each group were observed. ③ 2-3 mm thickness of hippocampal tissue with coronary section was taken for observing its ultrastructure under a transmission electron microscope. MAIN OUTCOME MEASURES: ① The ultrastructure of hippocampal tissue. ②The conscious state and electrical activity of brain of rats. RESULTS: Eighteen rats were enrolled in the final analysis. ① Observation of hippocampal ultrastructure: Electromicroscopic pathological findings showed that for each rat of the liquid nitrogen injured group, mitochondrium in the pyramidal neuron around the microgyrus was swelled, endoplasmic reticulum was expanded, glial cells were swelled, water gathered around the blood capillary, partial medullary sheath was degenerated, neuropilem was normal and no obviously abnormal synapse was found. ② Changes in conscious state of rats: Rats in the normal control group and sham-operation group had no convulsive seizure, but those in the liquid nitrogen injured group had occasionally. Most of them showed increased activities, excitation and restlessness, scratching and frequent ' watching face-like activities'. ③Electrical activity of brain of rats: Electroencephalogram recording of liquid nitrogen injured group showed that small wave amplitude of rhythm took the main part. No typical sharp wave, V wave, sharp and slow wave, V and slow waves were discharged. CONCLUSION: Liquid nitrogen can lead to cerebral cortical developmental disorder. Pathological changes of ultrastructure of focal tissue around the microgyrus can provide pathological basis for epilepsy associated with focal cortical developmental disorder. | Xuntai Ma Yong Yan Xuefeng Wang Chunyang Luo Xiaoping Wang Ning Yan Xiangqin Liu Yan Li | 2007 | Neural Regeneration Research2007，2，12： | 3 |
| 16 | Safe conservation and utilization of peanut germplasm resources in the Oil Crops Middle-term Genebank of China显示文摘Peanut(Arachis hypogaea L.)is an important oil and cash crop in the world.Peanut germplasm collected in China are abundant,which provides important material guarantee for peanut breeding and industrial development.Here,the safe conservation technology and indicators of peanut germplasm resources in the Oil Crops Middleterm Genebank of China were expounded from three processes of storage,monitoring,reproduction and renewal.We summarized and reviewed the situation of conservation and utilization of peanut germplasm resources in the Middle-term Genebank in the past 20 years.The future research direction of peanut resources in the Oil Crops Middle-term Genebank of China is prospected. | Xiaojing Zhou Xiaoping Ren Huaiyong Luo Li Huang Nian Liu Weigang Chen Yong Lei Boshou Liao Huifang Jiang | 2022 | Oil Crop Science2022，7，1： | 3 |
| 17 | Analysis of F_1 hybrid and BC_1 monosomic alien addition line plants from Brassica oleracea × Sinapis alba by GISH显示文摘Sterile and semi-fertile F_1 plants were obtained by intergeneric sexual hybridization between paternal Brassica oleracea var. alboglabra (genome CC, 2n=18) and maternal Sinapis alba (genome SS, 2n=24), BC_1 plants were obtained by backcrossing between paternal B. oleracea and ma-ternal semi-fertile F_1 plants. Genomic in situ hybridization (GISH) combined with dual-colour fluorescence in situ hybridization (dcFISH) showed that sterile F_1 plants contained 21 chromosomes consist-ing of one B. oleracea chromosome set and one S. alba chromosome set, belonging to expected hybrids, and semi-fertile F_1 plants contained 30 chromosomes consisting of two B. oleracea chromosome sets and one S. alba chromosome set. It is obvious that the semi-fertile F_1 plants belong to unexpected hybrids. 1―3 trivalents were detected at meiotic metaphase I of semi-fertile F_1 pollen mother cells (PMCs). Different separation ratios of S chromosomes were detected at anaphase I. A monosomic alien addition line (MAAL) was identified by GISH-dcFISH from BC_1 plants; it contained 19 chromosomes consisting of 18 C chromosomes and 1 S chromosome. At meiotic metaphase I, 9 divalents from B. oleracea and one univalent from S. alba could be detected. Sometimes, one putative C-S trivalent could also be detected. The achievement of B. oleracea-S. alba monosomic alien addition lines lays a foundation for gene introgression, location and cloning. | WEI Wenhui ZHANG Sufeng LI Jun WANG Lijun CHEN Bo FANG Xiaoping WANG Zhuan LUO Lixia | 2006 | Chinese Science Bulletin2006，51，23： | 3 |
| 18 | The detection of HBV DNA with gold nanoparticle gene probes显示文摘Objective: Gold nanoparticle Hepatitis B virus (HBV) DNA probes were prepared, and their application for HBV DNA mea-surement was studied. Methods: Alkanethiol modified oligonucleotide was bound with self-made Au nanoparticles to formnanoparticle HBV DNA gene probes, through covalent binding of Au-S. By using a fluorescence-based method, the number ofthiol-derivatized, single-stranded oligonucleotides and their hybridization efficiency with complementary oligonucleotides in solutionwas determined. With the aid of Au nanoparticle-supported mercapto-modified oligonucleotides serving as detection probes, andoligonucleotides immobilized on a nylon membrane surface acting as capturing probes, HBV DNA was detected visually by sand-wich hybridization based on highly sensitive aggregation and silver staining. The modified nanoparticle HBV DNA gene probeswere also used to detect the HBV DNA extracted from serum in patients with hepatitis B. Results: Compared with bare Aunanoparticles, oligonucleotide modified nanoparticles had a higher stability in NaCl solution or under high temperature environ-ment and the absorbance peak of modified Au nanoparticles shifted from 520nm to 524nm. For Au nanoparticles, the maximaloligonucleotide surface coverage of hexaethiol 30-mer oligonucleotide was (132 ±10) oligonucleotides per nanoparticle, and thepercentage of hybridization strands on nanoparticles was (22 ±3%). Based on a two-probe sandwich hybridization/nanoparticleamplification /silver staining enhancement method, Au nanoparticle gene probes could detect as low as 10-11 mol/L composite HBVDNA molecules on a nylon membrane and the PCR products of HBV DNA visually. As made evident by transmission electron mi-croscopy, the nanoparticles assembled into large network aggregates when nanoparticle HBV DNA gene probes were applied to de-tect HBV DNA molecules in liquid. Conclusion:Our results showed that successfully prepared Au nanoparticle HBV DNA geneprobes could be used to detect HBV DNA directly. The detection-visuallized method has many advantages, including high sensitivity,simple operation and low cost. This technique has potential applications in many fields, especially in multi-gene detection chips. | Dong Xia Xiaoping Luo Qin Ning Qianghua Lu Kailun Yao Zuli Liu | 2007 | Journal of Nanjing Medical University2007，21，4： | 2 |
| 19 | Organic–inorganic hybrid perovskite scintillators for mixed field radiation detection显示文摘Sensitive and fast detection of neutrons and gamma rays is vital for homeland security,high-energy physics,and proton therapy.Fast-neutron detectors rely on light organic scintillators,andγ-ray detectors use heavy inorganic scintillators and semiconductors.Efficient mixed-field detection using a single material is highly challenging due to their contradictory requirements.Here we report hybrid perovskites(C_(8)H_(12)N)_(2)Pb(Br_(0.95)Cl_(0.05))_(4)that combine light organic cations and heavy inorganic skeletons at a molecular level to achieve unprecedented performance for mixed-field radiation detection.High neutron absorption due to a high density of hydrogen,strong radiative recombination within the highly confined[PbX_(6)]^(4-)layer,and sub-nanometer distance between absorption sites and radiative centers,enable a light yield of 41000 photons/MeV,detection pulse width of 2.97 ns and extraordinary linearity response toward both fast neutrons andγ-rays,outperforming commonly used fast-neutron scintillators.Neutron energy spectrum,time-of-flight based fast-neutron/γ-ray discrimination and neutron yield monitoring were all successfully achieved using(C_(8)H_(12)N)_(2)Pb(Br_(0.95)Cl_(0.05))_(4)detectors.We further demonstrate the monitoring of reaction kinetics and total power of a nuclear fusion reaction.We envision that molecular hybridized scintillators open a new avenue for mixed-field radiation detection and imaging. | Mengling Xia Guangda Niu Linyue Liu Runlong Gao Tong Jin Pengying Wan Weicheng Pan Xianpeng Zhang Zuoxiang Xie Sam Teale Zenghua Cai Jiajun Luo Shan Zhao Haodi Wu Shiyou Chen Zhiping Zheng Qingguo Xie Xiaoping Ouyang Edward HSargent Jiang Tang | 2022 | InfoMat2022，4，9： | 2 |
| 20 | Relationship between Tanlu Fault and Hydrocarbon Accumulation in Liaozhong Sag, Bohai Bay, Eastern China显示文摘Tanlu fault(Liaozhong segment) goes straight through Liaodong Bay along NNE direction and it is divided into three segments, i.e., the northern segment, the middle segment and the southern segment, according to the differences in structural features. There are obvious changes in deposit thickness, sag structure, tectonic nature and other aspects of the layers. Tanlu fault is a special controlling factor concerning the reservoir formation in Liaozhong sag. First, its activities affect paleogeomorphology and paleogeographic framework and control the distribution of the sedimentary facies and then they proceed to control the distribution of the source rock and reservoir sand bodies. Second, its activities affect the formation and deformation of the structure, control the formation of abundant traps and cause the destruction of some traps. Third, its activities also affect the juxtaposition relation among the fault, the sand bodies and unconformity surfaces and control the function and efficacy of the three as the main hydrocarbon translocation system. In this paper the hydrocarbon reservoir formation process of JZ21-1, JX1-1 and LD27-2 oil and gas fields, the representatives of Tanlu strike slip fault zone are mainly analyzed. The modes of hydrocarbon reservoir formation can be generalized as follows—hydrocarbon source acts as 'soil' and oil and gas as 'nutrient'; hydrocarbon expulsion relies on 'roots'; hydrocarbon migration relies on 'trunks'; reservoir forms in 'brunches' and the whole process follows the pattern that 'hydrocarbon accumulates in strike slips and oil and gas reservoir forms like the growth of trees'. | Guosheng Xu Zhixing Xu Deyu Gong Donghong Zhou Changrong Wu Xiaoping Luo Jianping Li | 2014 | Journal of Earth Science2014，25，2： | 2 |
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