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| 1 | Dietary resveratrol improves antioxidant status of sows and piglets and regulates antioxidant gene expression in placenta by Keap1-Nrf2 pathway and Sirt1显示文摘Background: Resveratrol, a plant phenol, affords protection against inflammation and oxidative stress. The objective of this study was to investigate the effects of dietary resveratrol supplementation during pregnancy and lactation on the antioxidant status of sows and piglets and on antioxidant gene expression and pathway in placenta.Methods: Forty sows were allotted to 2 dietary treatments 20 d after breeding. Sows were fed a control diet and a control diet with 300 mg/kg resveratrol. Oxidative stress biomarkers and antioxidant enzymes were measured in the placenta, milk, and plasma of sows and piglets. Antioxidant gene expression and protein expression of Kelch-like ECH-associated protein 1-Nuclear factor E2-related factor 2(Keap1-Nrf2), nuclear factor kappa B-p65(NFκB-p65) and sirtuin1(Sirt1) were quantified in the placenta.Results: Dietary resveratrol increased the litter and piglets weaning weights. Antioxidant status in the milk, placenta and plasma of sows and piglets was partially improved by dietary resveratrol. In placenta, Nrf2 protein expression was increased and Keap1 protein expression was decreased by dietary resveratrol. The m RNA expression of antioxidant genes including catalase(CAT), glutathione peroxidase 1(GPX1), GPX4, superoxide dismutase 1(SOD1)and heme oxygenase 1(HO1), and phase 2 detoxification genes, including glutamate-cysteine ligase modifier(GCLM), microsomal glutathione S-transferase 1(MGST1) and UDP glucuronosyltransferase family 1 member A1(UGT1 A1), was increased by dietary resveratrol. Dietary resveratrol also increased Sirt1 and phosphorylated NFκB-p65 protein expression in the placenta. We failed to observe any influences of dietary resveratrol on pro-inflammatory cytokine levels, including those of interleukin 1β(IL-1β), IL-6, IL-8 and tumor necrosis factor α(TNF-α). However, we observed that the m RNA expression of IL-8 in placenta was reduced by maternal resveratrol. In addition, dietary resveratrol showed interactive effects with day of lactation on activities of SOD and CAT and levels of malonaldehyde(MDA) and hydrogen peroxide(H2 O2) in milk.Conclusions: Dietary resveratrol supplementation during pregnancy and lactation improves the antioxidant status of sows and piglets, which is beneficial to the reproductive performance of sows. Dietary resveratrol regulates placental antioxidant gene expression by the Keap1-Nrf2 pathway and Sirt1 in placenta. | Qingwei Meng Tao Guo Gaoqiang Li Shishuai Sun Shiqi He Baojing Cheng Baoming Shi Anshan Shan | 2018 | Journal of Animal Science and Biotechnology2018，9，3： | 33 |
| 2 | A Novel Single-Nucleotide Mutation in a CLAVATA3 Gene Homolog Controls a Multilocular Silique Trait in Brassica rapa L.显示文摘 | Chuchuan Fan, Yudi Wu, Qingyong Yang, Yang Yang, Qingwei Meng, Keqiao Zhang, Jinguo Li, Jinfang Wang Yongming Zhou | 2014 | Molecular Plant2014，7，12： | 12 |
| 3 | Nanosized-bismuth-embedded 1D carbon nanofibers as high-performance anodes for lithium-ion and sodium-ion batteries显示文摘因为它的高容量的能力,在潮湿 / 空气的稳定性,和灵巧的准备,双性人是精力存储材料的一个有希望的候选人。在这研究, nanosized-Bi-embedded 的电气化学的表演一个维(1D ) 碳 nanofibers (Bi/C nanofibers ) 系统地作为为 Li 离子电池(解放) 和 Na 离子电池(嘴) 的阳极被调查。Bi/C nanofibers 用一个单个嘴的 electrospinning 方法被准备,指定双性人来源由 carbothermal 减小列在后面。有约 20 nm 的直径的丰富的双性人 nanoparticles 同类地在 1D 碳 nanofibers 被驱散并且嵌入,由结构、词法的描述证实了。电气化学的大小显示 Bi/C nanofiber 阳极能为嘴为解放和更好的率表演交付长周期生活。Bi/C nanofiber 阳极的优异电气化学的表演被归因于 1D 碳 nanofiber 结构和在碳矩阵嵌入的双性人 nanoparticles 的一致分发。这唯一的嵌入的结构为卷变化引起的机械应力的有效版本提供一个有利电子搬运人和缓冲矩阵并且阻止双性人 nanoparticles 的聚集。 | Hong Yin Qingwei Li Minglei Cao Wei Zhang Han Zhao Chong Li Kaifu Huo Mingqiang Zhu | 2017 | Nano Research2017，10，6： | 12 |
| 4 | Randomized controlled multicenter clinical trial for integrated treatment of community-acquired pneumonia based on Traditional Chinese Medicine syndrome differentiation显示文摘OBJECTIVE:To evaluate the efficacy and safety of treatment based on syndrome differentiation ofTraditional Chinese Medicine(TCM) for community-acquired pneumonia(CAP).METHODS:A total of 240 CAP patients were randomly divided into the following two groups:thecontrol group was treated by anti-infection plus conventional medicine treatment;and the trial group was treated by TCM plus the above-mentioned treatment given to the controls.The course of treatment was 14 days,and the patients were followed up for 7 days.RESULTS:Of the 240 patients,235 accomplished the whole process of treatment.The five patients who withdrew from the study were brought into an intent-to-treat analysis.The therapeutic effects of the trial group were superior to those of the control group(P<0.01).The trial group took less time to become clinically stable,with a higher score in the quality of life(P<0.01).There were no significant differences in mortality rate(P>0.05),white blood cell count(P>0.05),bacterial clearance rate(P>0.05),and adverse reactions between the two integrated groups.CONCLUSION:Treatment based on TCM syndrome differentiation for CAP has the advantages of resulting in less time to achieve a stable clinical condition,improvement of clinical symptoms and quality of life,and is comparatively safe. | Jiansheng Li Xueqing Yu Suyun Li Haifeng Wang Yunping Bai Minghang Wang Zikai Sun Wei Zhang Zhaoshan Zhou Xianhua Jia Qingwei Zhou | 2012 | Journal of Traditional Chinese Medicine2012，32，4： | 11 |
| 5 | The role played by traditional Chinese medicine in preventing and treating COVID-19 in China显示文摘Traditional Chinese medicine(TCM),an ancient system of alternative medicine,played an active role in the prevention and control of COVID-19 in China.It improved the clinical symptoms of patients,reduced the mortality rate,improved the recovery rate,and effectively relieved the operating pressure on the national medical system during critical conditions.In light of the current global pandemic,TCM-related measures might open up a new channel in the control of COVID-19 in other countries and regions.Here,we summarize the TCM-related measures that were widely used in China,including TCM guidelines,the Wuchang pattern,mobile cabin hospitals,integrated treatment of TCM and modern medicine for critical patients,and non-medicine therapy for convalescent patients,and describe how TCM effectively treated patients afflicted with the COVID-19.Effective TCM therapies could,therefore,be recommended and practiced based on the existing medical evidence from increased scientific studies. | Qingwei Li Han Wang Xiuyang Li Yujiao Zheng Yu Wei Pei Zhang Qiyou Ding Jiaran Lin Shuang Tang Yikun Zhao Linhua Zhao Xiaolin Tong | 2020 | Frontiers of Medicine2020，14，5： | 10 |
| 6 | Detection of serum IgM and IgG for COVID-19 diagnosis显示文摘Dear Editor,Infection with the novel coronavirus(SARS-CoV-2,which is the virus responsible for the coronavirus disease 2019(COVID-19))was first reported in Wuhan,China on December 31,2019.The outbreak of COVID-19 remains ongoing and was linked to more than 80,000 infected patients and more than 3,000 deaths in China as of March 7,2020(Holshue et al.,2020). | Ling Zhong Junlan Chuan Bo Gong Ping Shuai Yu Zhou Yi Zhang Zhilin Jiang Dingding Zhang Xiaoqi Liu Shi Ma Yi Huang He Lin Qingwei Wang Lulin Huang Dan Jiang Fang Hao Juan Tang Chunqi Zheng Hua Yu Zhibin Wang Qi Jiang Tao Zeng Mei Luo Fanwei Zeng Fanxin Zeng Jianghai Liu Junxi Tian Yu Xu Tengxiang Long Kaiju Xu Xingxiang Yang Yuping Liu Yi Shi Li Jiang Zhenglin Yang | 2020 | Science China(Life Sciences)2020，63，5： | 8 |
| 7 | Knockdown of S-phase Kinase-associated Protein-2 Expression in MCF-7 Inhibits Cell Growth and Enhances the Cytotoxic Effects of Epirubicin显示文摘S 阶段联系 kinase 的 protein-2 (Skp2 ) 在调停 ubiquitin 的解朊作用起一个关键作用,导致房间从的前进一对一个 proliferative 状态静止。Skp2 完了在许多肿瘤表示了并且相反地与 p27 表示相关,包括乳癌。在这研究,我们使用了小介入的 RNA (siRNAs ) 在人的乳癌 MCF-7 细胞禁止 Skp2 表示并且如果击倒,从而由 RNA 干扰(RNAi ) Skp2 调查将禁止乳癌细胞生长并且在 MCF-7 细胞上影响 epirubicin 的效果。三 Skp2 siRNA 构造被重新结合并且短暂地 transfected 人乳癌 MCF-7 房间。Skp2 的下面规定被反向的抄写聚合酶链反应和蛋白质印迹分析证实。我们证明 Skp2 siRNA 转染减少了 Skp2 蛋白质并且在 MCF-7 房间导致了 p27 蛋白质的累积。Skp2 siRNA 在 vitro 并且在 vivo 禁止了房间增长。由 RNAi 的 Skp2 击倒在 vitro 的增加的细胞的 apoptosis。有治疗进一步与 epirubicin 跟随的 Skp2 siRNA 的治疗禁止了癌症房间线的增长,与 epirubicin 跟随的控制 siRNA 相比。因此, Skp2 击倒由 RNAi 的表示禁止乳癌细胞生长并且提高 epirubicin 的效果。Skp2 的调停 siRNA 的基因 silencing 能是为在规定下面的 p27 的抑制的新奇癌症基因治疗。 | Lichun SUN Li CAI Yan YU Qingwei MENG Xuesong CHENG Yanbin ZHAO Guangjie SUI Fengmin ZHANG | 2007 | Acta Biochimica et Biophysica Sinica2007，39，12： | 6 |
| 8 | The complete mitochondrial genomes sequences of Asio flammeus and Asio otus and comparative analysis显示文摘The complete mitochondrial genomes of Asio flammeus and Asio otus were se- quenced and found to span 18858 bp and 18493 bp, respectively. It is surprising to find the for- mer to be the largest among all avian mitochondrial genomes sequenced so far. The two ge- nomes have very similar gene order with that of Gallus gallus, neither contains the pseudo con- trol region, but both have a single extra base, namely Cytidine, at position 174 in ND3 gene. The control regions of Asio flammeus and Asio otus’ mitochondrial genomes span 3288 bp and 2926 bp respectively, which are the longest among vertebrates except for Myxine glutinosa and con- tribute to the large size of two genomes. The 3′end of the control region of Asio flammeus and Asio otus contains many tandemly repeated sequences, which are highly similar to a putative control element, i.e. Mt5, and may form stable stem-loop secondary structures. Such repeated sequences probably play an important role in regulating transcription and replication of mito- chondrial genome. Our results may provide important clues for uncovering the origin and evolu- tion mechanisms of mitochondrion genome. | SUN Yi1, MA Fei1, XIAO Bing1, ZHENG Junjie2, YUAN Xiaodong2, TANG Minqian2, WANG Li3, YU Yefei3 & Li Qingwei1 1. College of Life Sciences, Liaoning Normal University, Dalian 116029, China 2. TaKaRa Biotechnology (Dalian) Co., Ltd., Dalian 116600, China 3. The Administrative Office of the Natural Protective Zone of Snake Island-Lao Tie Hill, Dalian 116041, China Correspondence should be addressed to Li Qingwei | 2004 | Science China(Life Sciences)2004，47，6： | 6 |
| 9 | Clinical efficacy of comprehensive therapy based on traditional Chinese medicine patterns on patients with pneumoconiosis: a pilot double-blind, randomized, and placebo-controlled study显示文摘Effective therapy options for pneumoconiosis are lacking.Traditional Chinese medicine(TCM)presents a favorable prospect in the treatment of pneumoconiosis.A pilot study on TCM syndrome differentiation can evaluate the clinical efficacy and safety of TCM and lay a foundation for further clinical research.A double-blind,randomized,and placebo-controlled trial was conducted for 24 weeks,in which 96 patients with pneumoconiosis were randomly divided into the control and treatment groups.Symptomatic treatment was conducted for the two groups.The treatment group was treated with TCM syndrome differentiation,and the control group was treated with placebo.The primary outcomes were the six-minute walking distance(6MWD)and the St.George Respiratory Questionnaire(SGRQ)score.The secondary outcomes were the modified British Medical Research Council Dyspnea Scale(mMRC),Chronic Obstructive Pulmonary Disease Assessment Test(CAT),Hospital Anxiety and Depression Scale(HADS),and pulmonary function.Only 83 patients from the 96 patients with pneumoconiosis finished the study.For the primary outcome,compared with the control groups,the treatment group showed a significantly increased 6MWD(407.90 m vs.499.51 m;95%confidence interval(CI)47.25 to 135.97;P<0.001)and improved SGRQ total score(44.48 vs.25.67;95%CI 27.87 to 9.74;P<0.001).The treatment group also significantly improved compared with the control group on mMRC score(1.4 vs.0.74;95%CI 1.08 to 0.23;P=0.003),CAT score(18.40 vs.14.65;95%CI 7.07 to 0.43;P=0.027),and the total symptom score(7.90 vs.5.14;95%CI 4.40 to 1.12;P<0.001).No serious adverse events occurred.This study showed that TCM syndrome differentiation and treatment had a favorable impact on the exercise endurance and quality of life of patients with pneumoconiosis. | Jiansheng Li Hulei Zhao Yang Xie Jieya Li Qingwei Li Xuexin Chen Weiyu Zhang | 2022 | Frontiers of Medicine2022，16，5： | 5 |
| 10 | The Phytol Phosphorylation Pathway Is Essential for the Biosynthesis of Phylloquinone, which Is Required for Photosystem I Stability in Arabidopsis显示文摘Phytyl-diphosphate,在 tocopherol 和 phylloquinone 生合成作为普通底层提供 phytyl 一半,经由 phytol phosphorylation 源于 de novo isoprenoid 生合成或一条抢救小径。然而,很,很少为 phylloquinone 生合成对 phytyl 一半的角色和起源被知道。自从 VTE6, phytyl 磷酸盐 kinase,是为 phytol phosphorylation 的关键酶,我们描绘了 Arabidopsis vte6 异种在 photosystem 在 phylloquinone 生合成并且 phylloquinone 获得卓见进 phytyl 一半的角色我(希腊语的第二十三个字母) 生物的续生说。VTE6 猛烈异种 vte6-1 和 vte6-2 缺乏可检测的 phylloquinone,而在 VTE6 击倒的变异的 vte6-3 的 phylloquinone 内容是 90% 比那低在里面野类型。在 vte6 异种,希腊语的第二十三个字母功能被损害, PSI 建筑群的累积是有缺点的。希腊语的第二十三个字母核心子单元 PsaA/B 高效地在 vte6-3 被综合并且集合进 PSI 建筑群。然而,在装配希腊语的第二十三个字母建筑群的希腊语的第二十三个字母子单元的降级率比在在 vte6-3 是更快速的野类型。在 vte6-3,希腊语的第二十三个字母是更易受影响的加亮损坏比在里面野类型。我们的结果提供 phytol phosphorylation 小径为 phylloquinone 生合成是必要的,并且那 phylloquinone 为希腊语的第二十三个字母复杂稳定性被要求的第一条基因证据。 | Lei Wang Qingwei Li Aihong Zhang Wen Zhou Rui Jiang Zhipan Yang Huixia Yang Xiaochun Qin Shunhua Ding Qingtao Lu Xiaogang wen Congming Lu | 2017 | Molecular Plant2017，10，1： | 4 |
| 11 | Extraction of Hemicellulose from Acacia Wood via Autohydrolysis and Ethanol Precipitation显示文摘Isolation and utilization of hemicellulose are important processes in the pulp mill-based biorefinery. Considering the potential end use of the hemicellulose,this study investigated the concentration of monomeric and oligomeric sugars in the hydrolysate derived from autohydrolysis of acacia wood and the obtainment of these sugars via ethanol precipitation. The rate of generation of monomers and oligomers increased dramatically with an increase of the temperature from 160℃ to 180℃. The maximum content of oligomers and total sugars was achieved under conditions of 180℃ and 10 min,170℃ and 85 min,respectively. Interestingly,the maximum yield of hemicellulose via ethanol precipitation was achieved in a much shorter time,compared with the maximum yield of oligomer from the hydrolysate by autohydrolysis. A 6. 66 g / L quantity of hemicellulose was obtained by intensifying the conditions of ethanol precipitation. The hemicellulose characteristics were analyzed by Fourier transform infrared,nuclear magnetic resonance,and ultraviolet spectroscopy,and the molecular weight was determined by gel permeation chromatography and thermal analysis. | HaiQiang Shi KaiYuan Guo YanNing Sun Na Li Jian Zhang MeiHong Niu QingWei Ping | 2016 | Paper And Biomaterials2016，1，1： | 4 |
| 12 | Lamprey buccal gland secretory protein-2 (BGSP-2) inhibits human T lymphocyte proliferation显示文摘Lamprey is a representative of the agnathans, the most ancient class of vertebrates. Parasitic lampreys secrete anticoagulantfrom their buccal glands and prevent blood coagulation of host fishes. We identified a buccal gland secretory protein-2(BGSP-2) from a buccal gland cDNA library of Lampetra japonica. The full-length BGSP-2 gene was cloned and the recombinantBGSP-2 protein was generated. The role of BGSP-2 on lymphocyte proliferation was studied by examining its effects onhuman T lymphocytes. We found that lamprey BGSP-2 was able to effectively block the proliferation of T cells in vitro by inducingG_1/S cell cycle arrest. Furthermore, it inhibited the proliferation of human T lymphocytes stimulated by phytohemagglutinin(PHA) at a minimum concentration of 0.1μg/ml. Our data suggest that lamprey BGSP-2 is able to block the mitosis of humanT lymphocytes at the G1/S point, and has the potential of anti-proliferative effect on PHA-activated T | Jing SUN Shuiyan YU Zhuang XUE Cenjie LIU Yu WU Xin LIU Qingwei LI | 2010 | Current Zoology2010，56，2： | 3 |
| 13 | Restless legs syndrome following the use of ziprasidone: a case report显示文摘Restless legs syndrome(RLS)is a common sleep-related movement disorder characterised by an uncomfortable urge to move the legs that occurs during periods of inactivity.Although there have been many case reports on antipsychotic-induced RLS,ziprasidone has never been reported as a cause of RLS.We present a case of a female patient with schizophrenia who presented with symptoms of RLS following the administration of high doses of ziprasidone added to quetiapine and valproate.The patient’s symptoms of RLS occurred following the administration and titration of ziprasidone to 160 mg,and were relieved upon reducing the dose to 120 mg/day.Other potential causative medications and differential diagnoses that could have caused similar symptoms were excluded.Clinicians should be aware of the potential for ziprasidone-induced RLS.Dopamine and serotonin interaction could be the mechanism underlying ziprasidone-induced RLS. | Cuizhen Zhu Ran Bi Yuliang Hu Hui Zhou Daomin Zhu Brian Isaacson Qingwei Li Yezhe Lin | 2020 | General Psychiatry2020，33，2： | 3 |
| 14 | State-target strategy:a bridge for the integration of Chinese and Western Medicine显示文摘The methods of Traditional Chinese Medicine(TCM)’s diagnosis and treatment have undergone several changes.It is crucial to build a proper model which is capable to modernize TCM into a both standardized and individualized treatment.Tong xiao-lin proposed the state-target strategy to build a bridge for the integration of Chinese and Western medicine.It is a model based on modern medical disease concepts and using the method of TCM to balance the pathological states and adopting the achievements of pharmacology of Chinese medicine to focus on the disease targets,symptom targets,and biochemical indicator targets.The reconstruction of TCM diagnosis and treatment system for diabetes is a good example to demonstrate this theory.It could improve the clinical efficacy,support the scientific research,and reinforce the standardization of TCM. | GOU Xiaowen GAO Zezheng YANG Yingying LI Qingwei TONG Xiaolin CHEN Keyu LEI Ye SONG Bin ZHAO Linhua | 2021 | Journal of Traditional Chinese Medicine2021，41，1： | 3 |
| 15 | Dynamic linear calibration method for a wide range neutron flux monitor system in ITER显示文摘As a key part of the diagnosis system in the International Thermonuclear Experimental Reactor(ITER),the neutron flux monitor(NFM),which measures the neutron intensity of the fusion reaction,is a Counting-Campbelling system with a large dynamic counting range.A dynamic linear calibration method is proposed in this paper to solve the problem of cross-over between the different counting and Campbelling channels,and improve the accuracy of the cross-calibration for long-term operation.The experimental results show that the NFM system with the dynamic linear calibration system can obtain the neutron flux of the fusion reactor in real time and realize the seamless measurement area connection between the two channels. | LI Shiping XU Xiufeng CAO Hongrui YANG Qingwei YIN Zejie | 2013 | Nuclear Science and Techniques2013，24，4： | 3 |
| 16 | Modified halloysite nanotubes reduce the toxic effects of zearalenone in gestating sows on growth and muscle development of their offsprings显示文摘Background: Zearalenone(ZEN) is an estrogenic mycotoxin that is primarily produced by Fusarium fungi and has been proven to affect the reproductive capacity of many species to varying degrees. The present experiment was designed to study the maternal persistent effects of zearalenone toxicity in gestating sows on growth and muscle development of their offsprings, and the alleviation of zearalenone toxicity by modified halloysite nanotubes(MHNTs).Methods: Eighteen sows were fed with one of three dietary treatments that included the following:(1) a control diet,(2) a contaminated grain diet(with 50 % moldy corn, 2.77 mg/kg ZEN), and(3) a contaminated grain diet(with 50 %moldy corn, 2.76 mg/kg ZEN) + 1 % MHNTs. Each sow was exclusively fed its experimental diets from 35 to 70 d of gestation at a total of 2 kg daily. Muscle samples were collected from six piglets per treatment at birth, weaning and finishing.Results: The results showed that feeding the sows with the ZEN-contaminated diets from 35 to 70 d of gestation decreased the ADG, ADFI and G:F of their offsprings(P < 0.05). The muscle fiber numbers in the newborn, weaning and growing-finishing pigs and the muscle fiber diameters at birth and weaning were also decreased by maternal ZEN exposure(P < 0.05). The expressions of IGF-I, IGF-II, Myf-5 and Mstn at birth and IGF-II, Pax7, Myf-5 and Myo D1 at weaning were altered by feeding gestating sows with ZEN-contaminated diets(P < 0.05). The MHNTs reduced most of the ZEN-induced toxic effects: the ADG and ADFI on growth performance, the muscle fiber numbers at weaning and finishing and the muscle fiber diameters at weaning(P < 0.05). The expression levels of IGF-II and Mstn in newborn piglets and IGF-II and Myf-5 in weaning piglets were also prevented by adding 1 % MHNTs(P < 0.05).Conclusions: The present study demonstrated that the offsprings of sows fed with ZEN-contaminated diets from 35 to 70 day of gestation exhibited weakening on growth performance, physiological changes in their muscle fibers and alterations of m RNA expression in their muscle tissues, and also indicated that MHNTs prevented most of the ZENinduced weakening in the muscle tissues. | Rui Gao Qingwei Meng Jianan Li Min Liu Yuanyuan Zhang Chongpeng Bi Anshan Shan | 2016 | Journal of Animal Science and Biotechnology2016，7，4： | 3 |
| 17 | The Intronic cis Element SE1 Recruits trans-Acting Repressor Complexes to Repress the Expression of ELONGATED UPPERMOST INTERNODE1 in Rice显示文摘 | Yongyao Xie Yaling Zhang Jingluan Han Jikai Luo Gousi Li Jianle Huang Haibin WU Qingwei Tian Qinlong Zhu Yuanling Chen Yoji Kawano Yao-Guang Liu Letian Chen | 2018 | Molecular Plant2018，11，5： | 3 |
| 18 | Effects of the recombinant toxin protein rLj-RGD3 in multidrug-resistant human breast carcinoma cells显示文摘我们以前报导了那 Lj-RGD3,新奇 RGD 毒素蛋白质,从 Lampetra 的口腔的腺被孤立装饰用的梨树。recombinant 蛋白质 rLj-RGD3 有反侵略并且在肿瘤房间(HeLa 房间) 和在 vitro 的 endothelial 房间(ECV304 房间) 的反粘合剂活动,并且禁止 v3, v5,和 1 调停 integrin 的粘附。在这研究,我们在药抵抗的 MCF-7/Adr 胸癌细胞线和药敏感的父母线 MCF-7 调查了 rLj-RGD3 的 bioactivity,并且发现 rLj-RGD3 禁止了两根细胞线的生长。生物功能研究表明 rLj-RGD3 能在 MCF-7/Adr 导致 apoptosis,它在药敏感的父母线 MCF-7 是比那更流行的。另外, rLj-RGD3 禁止了 MCF-7/Adr 房间的粘附到 fibronectin。而且, rLj-RGD3 通过人工的 matrigel 地下室膜阻止了 MCF-7/Adr 房间的侵略。在摘要, rLj-RGD3 可以在 multidrug 抵抗的乳癌治疗被用作潜在的药。 | Jihong Wang Rong Xiao Xin Liu Fenfang Wu Yue Pang Bo Feng Donghui Yang Qingwei Li | 2012 | Acta Biochimica et Biophysica Sinica2012，44，5： | 3 |
| 19 | Cloning of the kininogen gene from Lampetra japonica provides insights into its phylogeny in vertebrates显示文摘Kininogens, the precursors of bradykinins, ubiquitously exist in vertebrates, including mammals, birds, amphibians, and fishes.To elucidate the phylogeny of kininogen genes in early vertebrates, we cloned the full-length cDNA of kininogen gene from the liver of Lampetra japonica.The open reading frame of this sequence contained 546 bp and encoded 181 amino acids, including a cystatin domain without the canonical binding site for cysteine proteinases and a bradykinin domain.Our results suggested that in lampreys and most of other vertebrates, there might be only one kininogen gene, which was fused by certain sequences during vertebrate evolution and encoded proteins with more functions;however, another special kininogen gene, only encoding the bradykinin domain with multiple copies in some species, arose only in amphibians for adapting themselves to the unique environment.Using reverse transcription PCR, kininogen mRNA was also detected in lamprey gut, kidney, and leukocyte, but absent in lamprey buccal gland.Our findings may provide insights into the phylogeny of kininogen genes as well as other gene families in vertebrates. | Liwei Zhou Xin Liu Ping Jin Qingwei Li | 2009 | Journal of Genetics and Genomics2009，36，2： | 3 |
| 20 | A novel Lyn-like protein tyrosine kinase identified in lamprey and its role in immune response显示文摘The avian sarcoma virus transforming gene(Src)kinase family,a type of nonmreceptor protein tyrosine kinases(nrTPKs),has been reported to play roles in cell growth,division,migration,adhesion,the immune response,and signal transduction berween cells[1,2].The Src family kinases can be divided into three subfamilies based on their conserved domains:SrcA subfamily(Sre,Yes,Fyn,and Fgr,SrcB subfamily(Lck,Hck,Blk,and Lyn),and Frk subfamily[3]. | Tingting Zhao Kedi Luo Xin Liu Shanshan Dong Yinglun Han Meng Gou Peng Su Qingwei Li Ting Zhu | 2020 | Acta Biochimica et Biophysica Sinica2020，52，5： | 2 |
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