|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells显示文摘Spermatogonial 干细胞(SSC ) 能在移植以后生产众多的男配偶子进接受者睾丸,介绍为基因治疗和修改基因的动物的连续生产的一条珍贵途径。然而, SSC 的成功的基因操作被限制了,部分由于复杂性和当前可得到的基因编辑技术的低效率。这里,我们证明有效基因修正能用 CRISPR-Cas9 系统被介绍进 SSC。我们使用了 CRISPR-Cas9 系统变异在 SCC 的 EGFP transgene 或内长的 Crygc 基因。变异的 SSC 在移植以后经历了精子发生进不肥沃的老鼠睾丸的生精的小管。圆 spermatids 被产生并且在进成熟卵母细胞的注射以后,支持了显示相应变异的显型的异质接合的后代的生产。而且,在 Crygc 的一个引起疾病的变化(在 SSC 先存在的 Crygc −/−) 能乐意地到加入的 CRISPR-Cas9-induced nonhomologous 结束(NHEJ ) 或指导相同的修理(HDR ) 被修理,导致没有是的离开目标修正的证据带改正的基因的 SSC 线由整个染色体的定序出现。用从这些线产生的圆 spermatids 的授精在 100% 的效率与改正的显型产生了后代。我们的结果表明有效基因由 CRISPR-Cas9 系统在老鼠 SSC 编辑,并且提供在 SSC 经由基因修正治好基因疾病的原则的证明。 | Yuxuan Wu Hai Zhou Xiaoying Fan Ying Zhang Man Zhang Yinghua Wang Zhenfei Xie Meizhu Bai Qi Yin Dan Liang Wei Tang Jiaoyang Liao Chikai Zhou Wujuan Liu Ping Zhu Hongshan Guo Hong Pan Chunlian Wu Huijuan Shi Ligang Wu Fuchou Tang Jinsong Li | 2015 | Cell Research2015，25，1： | 37 |
| 2 | Exosomal LINC00355 derived from cancer-associated fibroblasts promotes bladder cancer cell resistance to cisplatin by regulating miR-34b-5p/ABCB1 axis显示文摘Cisplatin resistance is a major challenge for bladder cancer(BC).Evidence indicates that exosome derived from cancer-associated fibroblasts(CAF-Exo)can promote chemotherapy resistance in various human tumors by delivering bioactive molecules.We have previously demonstrated that CAF-derived exosomal LINC00355 promotes BC cell proliferation and invasion.However,the underlying mechanisms are still unclear.In this study,we aimed to investigate the role and mechanisms of CAF-derived exosomal LINC00355 in BC cell resistance to cisplatin.Exosomes were isolated from normal fibroblasts(NFs)and BC tumor-derived CAFs,namely,NF-Exo and CAF-Exo.CAFs were transfected with si-Ctrl or si-LINC00355 and then different exosomes were isolated,namely,CAF^(si-Ctrl)-Exo and CAF^(si-LINC00355)-Exo.The human BC cell lines(T24 and 5367)were incubated with NF-Exo,CAF-Exo,CAF^(si-Ctrl)-Exo,and CAF^(si-LINC00355)-Exo in the presence of cisplatin.MTT proliferation assay and flow cytometric analysis showed that CAF-Exo promoted BC cell resistance to cisplatin and upregulated ABCB1 expression in BC cells by transferring LINC00355 to BC cells.Luciferase activity assay confirmed the interaction between miR-34b-5p and LINC00355 or ABCB1.qRT-PCR and western blot analysis further showed that LINC00355 sponged miR-34b-5p to upregulate ABCB1 expression.However,the promoting effects of CAF-Exo on BC cell resistance to cisplatin were abolished by miR-34b-5p overexpression and ABCB1 silencing.In conclusion,exosomal LINC00355 derived from CAFs promotes BC cell resistance to cisplatin by regulating the miR-34b-5p/ABCB1 axis. | Guangyue Luo Yangyang Zhang Zhonghui Wu Ligang Zhang Chaozhao Liang Xianguo Chen | 2021 | Acta Biochimica et Biophysica Sinica2021，53，5： | 6 |
| 3 | Exosomal LINC00355 derived from cancer-associated fibroblasts promotes bladder cancer cell proliferation and invasion by regulating miR-15a-5p/HMGA2 axis显示文摘We have previously demonstrated that exosomes derived from cancer-associated fibroblasts(CAFs)promote bladder cancer(BC)cell proliferation and invasion by transferring LINC00355.In this study,the molecular mechanisms underlying the pro-bladder cancer action of exosomal LINC00355 were explored.CAFs were obtained from BC tumor tissues,and normal fibroblasts(NFs)were obtained from adjacent normal tissues.Human BC cell lines(T24 and 5367)were incubated with NF-Exo(exosomes from NFs),CAF-Exo(exosomes from CAFs),CAFsi-Ctrl-Exo(exosomes from si-Ctrl-transfected CAFs),and CAFsi-LINC00355-Exo(exosomes from si-LINC00355-transfected CAFs).BC cell proliferation and invasion were evaluated by MTT and Transwell assays,respectively.The interaction between miR-15a-5p and LINC00355 or HMGA2 was examined by online bioinformatics analysis and luciferase activity assay.Results showed that HMGA2 is a direct target of miR-15a-5p,and LINC00355 functions as a sponge of miR-15a-5p to upregulate HMGA2 expression.The promoting effects of CAF-Exo on HMGA2 expression,cell proliferation,and cell invasion were hindered when LINC00355 expression was inhibited in BC cells.These promoting effects were also hindered when miR-15a-5p was overexpressed or HMGA2 was silenced in BC cells.In conclusion,exosomal LINC00355 derived from CAFs promotes BC cell proliferation and invasion by regulating miR-15a-5p/HMGA2 axis. | Yangyang Zhang Guangyue Luo Shinian You Ligang Zhang Chaozhao Liang Xianguo Chen | 2021 | Acta Biochimica et Biophysica Sinica2021，53，6： | 4 |
| 4 | Mode evolution and nanofocusing of grating-coupled surface plasmon polaritons on metallic tip显示文摘 | Fanfan Lu Wending Zhang Ligang Huang Shuhai Liang Dong Mao Feng Gao Ting Mei Jianlin Zhao | 2018 | Opto-Electronic Advances2018，1，6： | 3 |
| 5 | Co_9S_8 nanowires@NiCo LDH nanosheets arrays on nickel foams towards efficient overall water splitting显示文摘Water electrolysis is considered to be an effective way to fabricate hydrogen, and it is desirable to find the highly efficient, inexpensive and good durability bifunctional electrocatalysts for overall water splitting.In this paper, we synthesis a unique structured catalyst that was composed by Co_9S_8 nanowires and nickel cobalt layered double hydroxide(NiCo-LDH) nanosheets. The ultrathin nanosheets decorated on the Co_9S_8 nanoarrays offer large specific surface area, numerous active edge sites and excellent electrical conductivity for fast electron transfer. Benefiting from this heterogeneous structure, the catalyst presents excellent catalytic performance in alkaline media. It requires 168 mV to reach current density of 10 mA/cm^2 for HER and 278 m V to reach current density of 30 mA/cm^2 for OER. When used as electrode in a homemade two-electrode system, it only needs t a voltage of 1.63 V to achieve current densities of 10 mA/cm^2, which proves Co_9S_8@NiCo LDH/NF as a superior bifunctional catalyst for water splitting. | Jingan Yan Ligang Chen Xin Liang | 2019 | Science Bulletin2019，64，3： | 3 |
| 6 | Healthy soils for sustainable food production and environmental quality显示文摘Soil is the foundation for sustainable foodproduction and environmental protection. Created byunsustainable land management practices and a range ofsocial, economic and environmental drivers, soil degrada-tion and pollution have been an ongoing threat tointernational food security and environmental quality.Soil degradation and pollution assessments are, however,often focused on the soil itself with little scope to devisenew soil management approaches that match foodproduction systems and/or environmentalprotection.This study draws lessons from an Australia-China JointResearch Center Program, Healthy Soils for SustainableFood Production and Environmental Quality: a researchplatform that has brought together multi-disciplinaryapproaches fromworld-renowned universitiesandresearch organizations in Australia and China. To thisend, a framework is presented for future soil managementin a new way that combines excellence in research,industry and policymakers in a partnership that will ensurenot only the right focus of the research but also that high-quality outputs will be transferable to industry and end-users. | Xia LIANG Jizheng HE Fusuo ZHANG Qirong SHEN Jinshui WU lain MYOUNG Anthony GO'DONNELL Ligang WANG Enli WANG Julian HILL Deli CHEN | 2020 | Frontiers of Agricultural Science and Engineering2020，7，3： | 1 |
| 7 | Porcine methionine sulfoxide reductase B3:molecular cloning,tissue-specific expression profiles,and polymorphisms associated with ear size in Sus scrofa显示文摘Background: In Sus scrofa, methionine sulfoxide reductase B3(MSRB3) is a crucial candidate gene for ear size, and an important conformational trait of pig breeds. However, challenges in MSRB3 c DNA amplification have prevented further identification of MSRB3 allelic variants influencing pig ear size.Results: We cloned a full-length c DNA sequence of porcine MSRB3 by rapid-amplification of c DNA ends. The3,765-bp gene contained a 5'-untranslated region(UTR)(190 bp), a coding region(552 bp), and a 3'-UTR(3,016 bp) and shared 84 %, 84 %, 87 %, 86 %, and 70 % sequence identities with human, orangutan, mouse, chicken, and zebrafish,respectively. The gene encoded a 183-amino acid protein, which shared 88 %, 91 %, 89 %, 86 %, and 67 % identities with human, orangutan, mouse, chicken, and zebrafish, respectively. Tissue expression analysis using q RT-PCR revealed that MSRB3 was expressed in the heart, liver, lung, kidney, spleen, ear, muscle, fat, lymph, skeletal, and hypothalamic tissues. Three single nucleotide polymorphisms(SNPs) were identified in MSRB3: c.-735 C > T in the 5' flanking region,c.2571 T > C in the 3'-UTR, and a synonymous mutation of c.484 T > C in the coding region. The SNPs c.-735 C > T and c.2571 T > C were significantly associated with ear size in a Large White × Minzhu F2 population other than in Beijing Black pigs. Subsequently, at SNP c.-735 C > T, the m RNA of MSRB3 was significantly higher expressed in ears of individuals with the TT genotype(Minzhu) than those with CC(Large White).Conclusions: The porcine MSRB3 owned a 3,765-bp full-length c DNA sequence and was detected to express in ear tissue. Two SNPs of this gene were shown to be significantly associated with ear size in a Large White × Minzhu intercross population instead of Beijing Black pig population. What's more, the individuals with higher m RNA expression of MSRB3 have larger ear sizes. These results provide useful information for further functional analyses of MSRB3 influencing ear size in pigs. | Yuebo Zhang Jing Liang Longchao Zhang Ligang Wang Xin Liu Hua Yan Kebin Zhao Huibi Shi Tian Zhang Na Li Lei Pu Lixian Wang | 2016 | Journal of Animal Science and Biotechnology2016，7，2： | 1 |
| 8 | The investigation of an amidine-based additive in the perovskite films and solar cells显示文摘Here,we introduced acetamidine(C_2H_3N_2H_3,Aa)-based salt as an additive in the fabrication of perovskite(CH_3NH_3PW_3)layer for perovskite solar cells.It was found that as an amidine-based salt,this additive successfully enhanced the crystallinity of CH_3NH_3PW_3 and helped to form smooth and uniform films with comparable grain size and full coverage.Besides,perovskite film with additive showed a much longer carrier lifetime and an obviously enhanced open-circuit voltage in the corresponding devices,indicating that the acetamidine-based salt can reduce the carrier recombination in both the film and device.We further demonstrate a promising perovskite device based on acetamidine salt by using a configuration of ITOATiO_2/Perovskite/Spiro-OMeTAD/Au under < 150 ℃ fabrication condition.A power conversion efficiency(PCE)of 16.54%was achieved,which is much higher than the control device without acetamidine salt.These results present a simple method for film quality optimization of perovskite to further improve photovoltaic performances of perovskite solar cells,which may also benefit the exploration of A cation in perovskite materials. | Guanhaojie Zheng Liang Li Ligang Wang Xingyu Gao Huanping Zhou | 2017 | Journal of Semiconductors2017，38，1： | 1 |
| 9 | Experimental study on effects of moisture content on combustion characteristics of simulated municipal solid wastes in a fixed bed 显示文摘 | Liang Ligang Sun Rui Fei Jun | 2008 | Bioresource Technology2008，99，15： | 1 |
| 10 | Photoelectrochemical biofuel cell using porphyrin-sensitized nanocrystalline titanium dioxide mesoporous film as photoanode显示文摘 | Kunqi Wang Jing Yang Ligang Feng Yuwei Zhang Liang Liang Wei Xing Changpeng Liu | 2011 | Biosensors and Bioelectronics2011，，1： | 1 |
| 11 | hsa_circ_0062019 promotes the proliferation,migration,and invasion of prostate cancer cells via the miR-195-5p/HMGA2 axis显示文摘Circular RNA(circRNA)is a new class of non-coding RNA.It was reported that circRNA involves in the metastasis of cancer.The aim of this study is to explore the role and mechanism of circRNA hsa_circ_0062019 in the development of prostate cancer(PCa).Our results showed that hsa_circ_0062019 was highly expressed in PCa cell lines.Cell Counting Kit-8 assay revealed that upregulation of hsa_circ_0062019 boosted PCa cell proliferation,and silencing of hsa_circ_0062019 inhibited cell proliferation.Meanwhile,transwell assay proved that upregulation of hsa_circ_0062019 facilitated PCa cell invasion and migration,while downregulation of hsa_circ_0062019 inhibited these malignant phenotypes.Furthermore,luciferase reporter assay proved the binding of hsa_circ_0062019 with miR-195-5p and the binding between miR-195-5p and high mobility group AT-hook 2(HMGA2),suggesting that hsa_circ_0062019 promoted the expression of HMGA2 by sponging miR-195-5p.In addition,our results revealed that the hsa_circ_0062019-induced PCa cell malignant phenotypes were notably reversed by the downregulation of HMGA2.Overall,our study demonstrated that hsa_circ_0062019 promoted PCa cell proliferation,migration,and invasion via upregulation of HMGA2 expression by sponging miR-195-5p.Our study proved a novel molecular mechanism of PCa development and provided a potential target for the treatment of PCa. | Peiyu Wang Ligang Zhang Shuiping Yin Yuchen Xu Sheng Tai Li Zhang Chaozhao Liang | 2021 | Acta Biochimica et Biophysica Sinica2021，53，7： | 1 |
| 12 | Genome-wide search for candidate genes determining vertebrae number in pigs显示文摘Longer porcine carcasses may be expected to have more vertebrae. Therefore, vertebrae number in pigs is an economically important trait. To examine the genetic basis of this trait, we genotyped 578 F_2 Large White ?Minzhu pigs using the Porcine SNP60K Bead Chip. A genome-wide association study(GWAS) identified 36 significant single nucleotide polymorphisms(SNPs) on the chromosomes SSC1(294.28–300.32 Mb) and SSC7(102.22–109.39 Mb). A 6.04-Mb region that contained all 13 significant SNPs on SSC1 also contained the gene NR6A1, previously reported to influence the number of vertebrae in pigs. However, the reported putative casual mutation of NR6A1 c.748 C > T showed no genome-wide significant association with the trait, suggesting it was not a causal mutation in our population. The remaining 23 significant SNPs on SSC7 were concentrated in a 7.17-Mb region, which was within a quantitative trait locus interval for number of vertebrae. TMED10 was the closest gene to the most significant SNP and might be a candidate.Haplotype sharing and block analysis refined the QTL to an interval of about 3 Mb containing 29 candidate genes.Of these 29 genes, the previously reported possible casual mutation of VRTN g.19034 A > C was not found to be a causal mutation in our population. Exploration of these genes via additional genetic and functional studies in mammals revealed that TGFβ3 could be a good candidate on SSC7. A mutation of TGFβ3 c.1749 G > A was detected by GWAS and could be proposed as a candidate causal mutation, or as closely linked to a causal mutation, for the number of vertebrae in pigs. | Longchao ZHANG Jingwei YUE Xin LIU Jing LIANG Kebin ZHAO Hua YAN Na LI Lei PU Yuebo ZHANG Huibi SHI Ligang WANG Lixian WANG | 2017 | Frontiers of Agricultural Science and Engineering2017，4，3： | 1 |
| 13 | Experimental study on effects of moisture content on combustion characteristics of simulated municipal solid wastes in a fixed bed显示文摘 | Ligang Liang Rui Sun Jun Fei Shaohua Wu Xiang Liu Kui Dai Na Yao | 2007 | Bioresource Technology2007，，15： | 1 |
| 14 | Analysis of eightorganophosphorus pesticide residues in fresh vegetables retailed inagricultural product markets of Nanjing,China显示文摘 | Wang Ligang Liang Yongchao Jiang Xin | 2008 | Bull Environ Contam Toxicol2008，81，4： | 1 |
| 15 | Comparison of microwave ablation and multipolar radiofrequency ablation, both using a pair of internally cooled interstitial applicators: Results in ex vivo porcine livers显示文摘 | Xin Li Liang Zhang Weijun Fan Ming Zhao Ligang Wang Tian Tang Hua Jiang Jianlei Zhang Yanfeng Liu | 2011 | International Journal of Hyperthermia2011，，3： | 1 |
| 16 | Analysis of eight organophosphorus pesticide residues in fresh vegetables retailed in agricultural product markets of Nanjing, China显示文摘 | WANG LIGANG LIANG YONGCHAO JIANG XIN | 2008 | Bulletin Of Environmental Contamination And Toxicology2008，81，4： | 1 |
| 17 | High activity of Pd- W03/C catalyst as anodic catalyst for direct formic acid fuel cell显示文摘 | Feng Ligang Yan Liang Cui Zhiming | 2011 | J Power Sources2011，196，5： | 1 |
| 18 | 3D Printing Challenges in Enabling Rapid Response to Public Health Emergencies显示文摘The coronavirus disease 2019(COVID-19)epidemic has become a global public health emergency over last months.As of September 14th,over 28,000,000 confirmed cases and 917,000 deaths have been reported from all over the world.1 Its large-scale outbreak and pandemic cause an extreme shortage of personal protective equipment(PPE)andmedical supplies such as face masks,testing kits,nasopharyngeal swabs,and medical ventilators in the early stage,which consequently leads to the collapse of local medical systems.2 It turns out that measures like lockdown and travel bans are themost effective strategies in response to an epidemic at an early stage with insufficient knowledge.3 However,those physical isolation measures will inevitably result in work stoppages and have a great impact on industrial production.The supply of raw materials and components,and the production and transportation of final products are greatly restricted under a global supply chain.4 This further aggravates the shortage of anti-epidemic supplies and so the vicious cycle begins. | Dingyi Wang Junpeng Zhang Qian Liu Bolei Chen Yong Liang Ligang Hu Guibin Jiang | 2020 | The Innovation2020，1，3： | 0 |
| 19 | Targeted drug delivery strategies for the treatment of rheumatoid arthritis显示文摘A chronic autoimmune disease named rheumatoid arthritis(RA)is characterized mainly by synovitis,which has high morbidity,disability and mortality rates.In addition,the aetiology and pathogenesis of RA have not been fully determined.RA is usually accompanied by persistent arthritic pain,swelling,stiffness,and functional disorders;in severe cases,it may result in cardiovascular,pulmonary,psychological and skeletal diseases(Scott et al.,2010). | Jinxia Zhang Ling Jiang Lihong Sun Ping Wang Suhui Sun Menghong Xu Lulu zhang Shumin Wang Xiaolong Liang Ligang Cui | 2021 | Science China(Life Sciences)2021，64，7： | 0 |
| 20 | Advances in the Application of Perovskite Materials显示文摘Nowadays, the soar of photovoltaic performance of perovskite solar cells has set off a fever in the study of metal halide perovskite materials. The excellent optoelectronic properties and defect tolerance feature allow metal halide perovskite to be employed in a wide variety of applications. This article provides a holistic review over the current progress and future prospects of metal halide perovskite materials in representative promising applications, including traditional optoelectronic devices(solar cells, light-emitting diodes, photodetectors, lasers), and cutting-edge technologies in terms of neuromorphic devices(artificial synapses and memristors) and pressure-induced emission. This review highlights the fundamentals, the current progress and the remaining challenges for each application, aiming to provide a comprehensive overview of the development status and a navigation of future research for metal halide perovskite materials and devices. | Lixiu Zhang Luyao Mei Kaiyang Wang Yinhua Lv Shuai Zhang Yaxiao Lian Xiaoke Liu Zhiwei Ma Guanjun Xiao Qiang Liu Shuaibo Zhai Shengli Zhang Gengling Liu Ligang Yuan Bingbing Guo Ziming Chen Keyu Wei Aqiang Liu Shizhong Yue Guangda Niu Xiyan Pan Jie Sun Yong Hua Wu‑Qiang Wu Dawei Di Baodan Zhao Jianjun Tian Zhijie Wang Yang Yang Liang Chu Mingjian Yuan Haibo Zeng Hin‑Lap Yip Keyou Yan Wentao Xu Lu Zhu Wenhua Zhang Guichuan Xing Feng Gao Liming Ding | 2023 | Nano-Micro Letters2023，15，10： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员