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| 1 | Gene cloning and expression of cadherin in midgut of Helicoverpa armigera and its Cry1A binding region显示文摘Cadherins belong to one of the families of animal glycoproteins responsible for cal-cium-dependent cell-cell adhesion. Recent literatures showed that the cadherin-like in midgut of several insects served as the receptor of Bt toxin Cry1A and the variation of cadherin-like is re-lated to insect’s resistance to Cry1A. The full-length cDNA encoding cadherin-like of Helicoverpa armigera is cloned by degenerate PCR and RACE techniques and the gene was designated as BtR-harm, which is 5581 bp in full-length, encoding 1730 amino acid residues (BtR-harm was deposited in GenBank and the accession number is AF519180). Its predicted molecular weight and isoelectric point were 195.39 kDa and 4.23, respectively. The inferred amino acid sequence includes a signal sequence, 11 cadherin repeats, a membrane-proximal region, a transmem-brane region and a cytoplasmic region. Sequence analysis indicated that the deduced protein sequence was most similar to the cadherin-like from Heliothis virescens with 84.2% identity and highly similar to three other lepidopteran cadherin from Bombyx mori, Manduca sexta and Pectinophora gossypiella, with the sequence identities of 60.3.6%, 57.5% and 51.0%, respec-tively. The cDNA encoding cadherin gene was expressed successfully in E. coli and the recom-binant proteins can bind with Cry1Ac. Truncation analysis and binding experiment of BtR-harm revealed that the Cry1A binding region was a contiguous 244-amino acid sequence, which lo-cated between amino acid 1217 and 1461. Semi-quantitative RT-PCR analysis showed that BtR-harm was highly expressed in midgut of H. armigera, very low expressed in foregut and hindgut and was not expressed in other tissues. After H. armigera producing resistance to Cry1Ac, the expression quantity of BtR-harm significantly decreased in midgut of H. armigera. It is the first confirmation that BtR-harm can function as receptor of Cry1Ac in H. armigera and the binding region was located on a contiguous 244 amino acid sequence, suggesting that the de-crease of expression quantity of BtR-harm is one of the main reasons for H. armigera resistance to Cry1Ac. | WANG Guirong WU Kongming LIANG Gemei GUO Yuyuan | 2005 | Science China(Life Sciences)2005，48，4： | 17 |
| 2 | Chinese Stroke Association guidelines for clinical management of cerebrovascular disorders:executive summary and 2019 update of the management of high-risk population显示文摘Aim Cerebrovascular disease is the leading cause of death and disability in China,causing a huge burden among patients and their families.Hence,stroke prevention is critical,especially in the high-risk population.Here,we present the evidence-based guideline suitable for the Chinese population.Methods Literature search of PubMed and Cochrane library(from January 1964 to June 2019)was done.After thorough discussion among the writing group members,recommendations were listed and summarised.This guideline was reviewed and discussed by the fellow writing committees of the Chinese Stroke Association’s Stroke.Results This evidence-based guideline was written in three parts:controlling the risk factors of stroke,utilisation of antiplatelet agents and assessing the risks of first-ever stroke.All recommendations were listed along with the recommending classes and levels of evidence.Conclusions This guideline provides recommendations for primary prevention of cerebrovascular disease among high-risk population in China.Controlling related risk factors,appropriately using antiplatelet agents,assessing the risk of developing first-ever stroke should help reduce the rate of cerebrovascular disease in China. | Yilong Wang Shangrong Han Haiqiang Qin Huaguang Zheng Bin Jiang Yong Cao Yuan Gao Ling Guan Qian Jia Yong Jiang Yuming Jiao Shuya Li Yapeng Li Zixiao Li Wei Liu Xiaojuan Ru Dongling Sun Haixin Sun Penglian Wang Tingting Wang Lixia Zong Lei Guo Xuewei Xie Yuming Xu Yuyuan Xu Xiaomeng Yang Yingying Yang Mengyuan Zhou Wenzhi Wang Chinese Stroke Association Stroke Council Guideline Writing Committee | 2020 | Stroke & Vascular Neurology2020，5，3： | 11 |
| 3 | Cloning,expression and binding specificity analysis of odorant binding protein 3 of the lucerne plant bug,Adelphocoris lineolatus(Goeze)显示文摘Credible evidence shows that odorant binding proteins(OBPs)are required for insect olfaction perception and play a key role in transporting hydrophobic odorants across the sensillum lymph to the olfactory receptors(ORs).In the present study,a novel OBP(AlinOBP3)gene from the lucerne plant bug,Adelphocoris lineolatus,was cloned and expressed.The expression pattern of AlinOBP3 was evaluated by qPCR,which indicated that AlinOBP3 was dominantly expressed in antennae.The binding properties of AlinOBP3 with 9 cotton volatiles and 5 sex pheromone analogs were measured by fluorescence competitive binding assays with the fluorescence probe 1-NPN.The results revealed that of 9 cotton volatiles,Myrcene,β-Ocimene and α-Phellandrene can bind with AlinOBP3.α-Phellandrene especially bound to AlinOBP3 with a high binding affinity,with a dissociation constant of 56.68 μmol/L.Of the 5 sex pheromone analogs,Hexyl butyrate had the strongest binding affinity with AlinOBP3,with a dissociation constant as 59.53 μmol/L.Butyl butyrate,trans-2-Hexenyl butyrate and Ethyl butyrate had medium binding affinities with AlinOBP3,with dissociation constants of 227.39,108.77 and 143.47 μmol/L,respectively.The results suggest that AlinOBP3 might be a pheromone binding protein(PBP)with a dual-function for the perception of sex pheromones and plant volatiles. | GU ShaoHua SUN Yang REN LiYan ZHANG XueYing ZHANG YongJun WU KongMing GUO YuYuan | 2010 | Chinese Science Bulletin2010，55，34： | 7 |
| 4 | The bacterial abundance and production in the East China Sea: seasonal variations and relationships with the phytoplankton biomass and production显示文摘The East China Sea is a productive marginal sea with a wide continental shelf and plays an important role in absorbing atmospheric carbon dioxide and transferring terrigenous organic matter to the open ocean. To investigate the roles of heterotrophic bacteria in the biogeochemical dynamics in the East China Sea, bacterial biomasses(BB) and productions(BP) were measured in four cruises. The spatial distributions of the BB and the BP were highly season-dependent. Affected by the Changjiang River discharge, the BB and the BP were high in shelf waters(bottom depth not deeper than 50 m) and generally decreased offshore in August 2009. In December 2009 to January 2010, and November to December 2010, the BB and the BP were high in waters with medium bottom depth. The onshore-offshore decreasing trends of the BB and the BP also existed in May–June 2011, when the BB was significantly higher than in other cruises in shelf break waters(bottom depth deeper than 50 m but not deeper than 200 m). The results of generalized additive models(GAM) suggest that the BB increased with the temperature at a range of 8-20°C, increased with the chlorophyll concentration at a range of 0.02–3.00 mg/m3 and then declining, and decreased with the salinity from 28 to 35. The relationship between the temperature and the log-transformed bacterial specific growth rate(SGR) was linear. The estimated temperature coefficient(Q10) of the SGR was similar with that of the phytoplankton growth. The SGR also increased with the chlorophyll concentration. The ratio of the bacterial to phytoplankton production ranged from less than 0.01 to 0.40, being significantly higher in November–December 2010 than in May–June 2011. Calculated from the bacterial production and growth efficiency, the bacterial respiration consumed, on average, 59%, 72% and 23% of the primary production in August 2009, November–December 2010, and May–June 2011, respectively. | CHEN Bingzhang HUANG Bangqin XIE Yuyuan GUO Cui SONG Shuqun LI Hongbo LIU Hongbin | 2014 | Acta Oceanologica Sinica2014，33，9： | 2 |
| 5 | Cytotoxicity and binding profiles of activated CrylAc and Cry2Ab to three insect cell lines显示文摘 | Jizhen Wei Gemei Liang Kongming Wu Shaohua Gu Yuyuan Guo Xinzhi Ni Xianchun Li | 2018 | Insect Science2018，25，4： | 2 |
| 6 | Resistance alleviation in the larval cotton bollworm to fenvalerate after pretreatment with Bacillus thuringiensis显示文摘 | Tan Weijia Liang Gemei Guo Yuyuan | 1999 | Entomologia Sinica1999，6，1： | 1 |
| 7 | Toxicity of chlorantraniliprole to Cry1Ac-susceptible and resistant strains of Helicoverpa armigera显示文摘 | Guangchun Cao Qiong Lu Lili Zhang Fang Guo Gemei Liang Kongming Wu Kris A.G. Wyckhuys Yuyuan Guo | 2010 | Pesticide Biochemistry and Physiology2010，，1： | 1 |
| 8 | Partial Cloning and characterization of the cDNA of general odorant binding protein 1 gene in the antenna of Helicoverpa armigera显示文摘 | Wang Guirong Wu Kongming Guo Yuyuan | 2001 | Entomologia Sinica2001，8，4： | 1 |
| 9 | Adsorption behavior of activated carbon for the elimination of zearalenone during bleaching process of corn oil显示文摘Zearalenone is a mycotoxin produced by Fusarium species.It frequently contaminates cereals used for foods or animal feeds,especially deposited in crude corn oil.Certain amounts of zearalenone can be removed during refining processes.In this study,we studied the influence of activated carbon and six industial absorbents(zeolite,diatomite,attapulgite,perlite,montmorillonite and activated clay)on the elimination of zearalenone during bleaching process of corn oil and explored the absorption mechanism of activated carbon.Results showed that activated carbon had an excellent adsorption capacity of zearalenone compared with the other six industrial adsorbents.For activated carbon,a high removal rate of zearalenone(exceeding 83%)from heavily zearalenone-polluted corn oil was achieved and the removal rate of zearalenone was kept above 60%after five regeneration cycles.The research on the adsorption mechanism of activated carbon showed that Freundlich adsorption isotherm model and pseudo-second-order kinetic model could well described the adsorption process.The thermodynamic study demonstrated that adsorption process was spontaneous and exothermic.Fourier transform infrared spectroscopy and Raman spectroscopy further revealed that activated carbon was effectively combined with zearalenone viaπ-πinteraction.Thus,activated carbon is an efficient and suitable adsorbent to control the levels of zearalenone during bleaching process of corn oil.This study not only proposed a systematic research scheme for the mechanism study of activated carbon for the elimination of zearalenone in corn oil,but also provided the scientific basis for developing effective methods to eliminate zearalenone in refined vegetable oils. | Yuyuan Hu Chuanguo Ma Weifeng Huang Shujing Guo Tong Wang Jun Liu | 2023 | Grain & Oil Science and Technology2023，6，1： | 1 |
| 10 | An experimental investigation on performance of bubble pump with lunate channel for absorption refrigeration system显示文摘 | Lianying Zhang Yuyuan Wu Hongfei Zheng Jingang Guo Dongsheng Chen | 2005 | International Journal of Refrigeration2005，，5： | 1 |
| 11 | Evalua tion of the natural refuge function for Helicoverpa armigera (Lepidoptera:Noctuidae) within Bacillus thuringiensis transgenic cotton growing areas in north China显示文摘 | Wu Kongming Guo Yuyuan Gao Shansong | | 0，，04： | 1 |
| 12 | TLR9 and BAFF: Their expression in patients with IgA nephropathy显示文摘 | Weiwei Li Xiaofei Peng Yuyuan Liu Hong Liu Fuyou Liu Liyu He Yang Liu Fan Zhang Chunyan Guo Guochun Chen Lei Zhang Zheng Dong Youming Peng | 2014 | Molecular Medicine Reports2014，，3： | 1 |
| 13 | Effects of host plant on susceptibility to deltamethrin and detoxication enzymes of Heliothis armigera(Lepidoptera;Noctuidae)显示文摘 | TAN WEIJIA GUO YUYUAN | 1996 | J Econ Entomol1996，89，1： | 1 |
| 14 | Enhanced trimeric ACE2 exhibits potent prophylactic and therapeutic efficacy against the SARS-CoV-2 Delta and Omicron variants in vivo显示文摘Dear Editor,The outbreak of coronavirus disease 2019(COVID-19)has resulted in a severe global pandemic that has lasted for more than two years.1 Several vaccines were developed at an unprecedented speed to protect billions of people.2 Many monoclonal antibodies have also been discovered,several of which are in the clinical stages.3 However,limited vaccine availability and vaccine hesitancy compromise the impact of vaccines.The continuous evolution of SARS-CoV-2 has also resulted in many variants that can escape immunity,reducing the effects of vaccines and antibodies.4 With more than 30 mutations in the spike protein,the Omicron variant escapes the majority of existing antibodies and extensively evades vaccine-induced immunity.5,6 Thus,therapeutics that are broadly effective against current and future emerging SARS-CoV-2 variants are still highly desirable. | Mengjiao Li Zi-Wei Ye Kaiming Tang Liang Guo Wenwen Bi Yuyuan Zhang Yan-dong Tang Guoguang Rong Mohamad Sawan Xin Yin Ren Sun Shuofeng Yuan Bobo Dang | 2022 | Cell Research2022，32，6： | 1 |
| 15 | Hydrophilic phytosterol derivatives: A short review on structural modifications, cholesterol-lowering activity and safety显示文摘Phytosterols have received extensive attention owing to their excellent cholesterol-lowering activity and the role in cardiovascular diseases prevention. However, poor solubility in both oil and water limited the application of free phytosterols in the food industry. Chemical or enzymatic modifications were effective to improve the physicochemical properties as well as the bioavailability and cholesterollowering activity of phytosterols. Higher oil solubility and lower melting point of phytosterols have been achieved by esterification and transesterification with fatty acids and triacylglycerols so as to enhance the bioavailability, reduce formation of precipitates, and improve the sensory quality of products.While the researches on the improvement on its water solubility is a hot topic. Hydrophilic phytosterol derivatives have promising applications in the food industry because most of foods belong to aqueous matrix. Hydrophilic modification is useful and meaningful for phytosterols in both industrial and commercial applications. This review mainly highlights the hydrophilic phytosterol derivatives in the following aspects:(i) hydrophilic modifications of phytosterols by coupling with various polar components;(ii) cholesterol-lowering activity and possible molecular mechanisms of hydrophilic phytosterol derivatives on reducing serum cholesterol level;and(iii) safety evaluation of hydrophilic phytosterol derivatives in cell-culture studies, animal models and clinical trials. | Yuyuan Hu Chuanguo Ma Xiaowei Chen Ge Bai Shujing Guo | 2022 | Grain & Oil Science and Technology2022，5，3： | 1 |
| 16 | The evolution of cotton pest management practice in China显示文摘 | Wu Kongming Guo Yuyuan | 2005 | Annu Rev Entomol2005，50，： | 1 |
| 17 | The evolution of cotton pest management practices in China 显示文摘 | Wu Kongming Guo Yuyuan | 2005 | Annual Review of Ento- mology2005，50，： | 1 |
| 18 | Melatonin decreases GSDME mediated mesothelial cell pyroptosis and prevents peritoneal fibrosis and ultrafiltration failure显示文摘Peritoneal fibrosis together with increased capillaries is the primary cause of peritoneal dialysis failure.Mesothelial cell loss is an initiating event for peritoneal fibrosis.We find that the elevated glucose concentrations in peritoneal dialysate drive mesothelial cell pyroptosis in a manner dependent on caspase-3 and Gasdermin E,driving downstream inflammatory responses,including the activation of macrophages.Moreover,pyroptosis is associated with elevated vascular endothelial growth factor A and C,two key factors in vascular angiogenesis and lymphatic vessel formation.GSDME deficiency mice are protected from high glucose induced peritoneal fibrosis and ultrafiltration failure.Application of melatonin abrogates mesothelial cell pyroptosis through a MT1R-mediated action,and successfully reduces peritoneal fibrosis and angiogenesis in an animal model while preserving dialysis efficacy.Mechanistically,melatonin treatment maintains mitochondrial integrity in mesothelial cells,meanwhile activating m TOR signaling through an increase in the glycolysis product dihydroxyacetone phosphate.These effects together with quenching free radicals by melatonin help mesothelial cells maintain a relatively stable internal environment in the face of high-glucose stress.Thus,Melatonin treatment holds some promise in preserving mesothelium integrity and in decreasing angiogenesis to protect peritoneum function in patients undergoing peritoneal dialysis. | Hongxia Ruan Xuejuan Li Lina Zhou Zihan Zheng Rulin Hua Xu Wang Yuan Wang Yujie Fan Shuwen Guo Lihua Wang Shafiq ur Rahman Ziwei Wang Yuyuan Wei Shuangyan Yu Rongzhi Zhang Qian Cheng Jie Sheng Xue Li Xiaoyan Liu Ruqiang Yuan Xiaoyan Zhang Lihong Chen Guowang Xu Youfei Guan Jing Nie Hongqiang Qin Feng Zheng | 2024 | Science China(Life Sciences)2024，67，2： | 0 |
| 19 | Illustrations with Real Examples of Using Ecological Regulation Strategies Against Crop Pests in China显示文摘In this paper, a series of examples from China, including those using ecological regulation strategies successfully to keep crop pests under control and those making more damages of crop pests caused by wrong cropping systems, crop distributions or extensive cultivation, were given and analyzed. From these, two pieces of experience were enlightened as follows: the first, the reforms of cropping systems and crop distributions must be beneficial to pest control or at least not increase the damage of crop pests; the second, we should further clarify the relationship between population dynamics of pests and cropping system/crop distribution, the basis for correctly laying down the ecological regulation systems. | Guo Yuyuan Liang Gemei | 2006 | 工程科学(英文版)2006，4，4： | 0 |
| 20 | Intrinsic persistent room temperature phosphorescence derived from 1H-benzo[f]indole itself as a guest显示文摘The influence of 1H-benzo[f]indole(Bd) and its derivatives on room temperature phosphorescence(RTP)has raised great concern since they were found to significantly affect RTP of the extensively studied carbazole(Cz) derivatives. However, the role of Bd itself existing in Cz-based or other doping systems was still unclear. In order to clarify its intrinsic phosphorescent property, Bd was introduced as a guest into different organic matrixes including substituted Cz derivatives and polymers. The phosphorescence located in 560–620 nm was confirmed to be derived from Bd itself, which can be detected whatever Bd was doped in the crystal or amorphous state of Cz derivatives. The suitable energy gap between Cz derivatives and Bd is the key to achieve ultralong RTP of Bd. Additionally, when doped in polymers with plenty of hydrogen bonds, RTP of Bd with lifetime over 280 ms was easily obtained. Among them, Bd@PHEMA(poly(hydroxyethyl methacrylate) exhibited superior phosphorescence, with yellow afterglow lasting for over 2.5 s. Therefore, this work demonstrated that a new organic RTP phosphor, Bd, is discovered, and ultralong RTP of Bd can be achieved not only doped in Cz derivatives but also in polymers as the hosts. | Danman Guo Yuyuan Wang Jinzheng Chen Yifeng Cao Yiling Miao Huahua Huang Zhenguo Chi Zhiyong Yang | 2023 | Chinese Chemical Letters2023，34，7： | 0 |
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