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| 1 | Cloning,expression and binding specificity analysis of odorant binding protein 3 of the lucerne plant bug,Adelphocoris lineolatus(Goeze)显示文摘Credible evidence shows that odorant binding proteins(OBPs)are required for insect olfaction perception and play a key role in transporting hydrophobic odorants across the sensillum lymph to the olfactory receptors(ORs).In the present study,a novel OBP(AlinOBP3)gene from the lucerne plant bug,Adelphocoris lineolatus,was cloned and expressed.The expression pattern of AlinOBP3 was evaluated by qPCR,which indicated that AlinOBP3 was dominantly expressed in antennae.The binding properties of AlinOBP3 with 9 cotton volatiles and 5 sex pheromone analogs were measured by fluorescence competitive binding assays with the fluorescence probe 1-NPN.The results revealed that of 9 cotton volatiles,Myrcene,β-Ocimene and α-Phellandrene can bind with AlinOBP3.α-Phellandrene especially bound to AlinOBP3 with a high binding affinity,with a dissociation constant of 56.68 μmol/L.Of the 5 sex pheromone analogs,Hexyl butyrate had the strongest binding affinity with AlinOBP3,with a dissociation constant as 59.53 μmol/L.Butyl butyrate,trans-2-Hexenyl butyrate and Ethyl butyrate had medium binding affinities with AlinOBP3,with dissociation constants of 227.39,108.77 and 143.47 μmol/L,respectively.The results suggest that AlinOBP3 might be a pheromone binding protein(PBP)with a dual-function for the perception of sex pheromones and plant volatiles. | GU ShaoHua SUN Yang REN LiYan ZHANG XueYing ZHANG YongJun WU KongMing GUO YuYuan | 2010 | Chinese Science Bulletin2010，55，34： | 7 |
| 2 | Electron-Deficient Zn-N_(6) Configuration Enabling Polymeric Carbon Nitride for Visible-Light Photocatalytic Overall Water Splitting显示文摘Despite of suitable band structures for harvesting solar light and driving water redox reactions,polymeric carbon nitride(PCN)has suffered from poor charge transfer ability and sluggish surface reaction kinetics,which limit its photocatalytic activity for water splitting.Herein,atomically dispersed Zn-coordinated three-dimensional(3D)sponge-like PCN(Zn-PCN)is synthesized through a novel intermediate coordination strategy.Advanced characterizations and theoretical calculations well evidence that Zn single atoms are coordinated and stabilized on PCN in the form of Zn-N_(6) configura-tion featured with an electron-deficient state.Such an electronic configuration has been demonstrated contributive to promoted electron excitation,accelerated charge separation and transfer as well as reduced water redox barriers.Further benefited from the abundant surface active sites derived from the 3D porous structure,Zn-PCN realizes visible-light photocatalysis for overall water splitting with H_(2) and O_(2) simultaneously evolved at a stoichiometric ratio of 2:1.This work brings new insights into the design of novel single-atom photocatalysts by deepening the understanding of electronic configurations and reactive sites favorable to excellent photocatalysis for water splitting and related solar energy conversion reactions. | Daming Zhao Yiqing Wang Chung-Li Dong Fanqi Meng Yu-Cheng Huang Qinghua Zhang Lin Gu Lan Liu Shaohua Shen | 2022 | Nano-Micro Letters2022，14，12： | 2 |
| 3 | Cytotoxicity and binding profiles of activated CrylAc and Cry2Ab to three insect cell lines显示文摘 | Jizhen Wei Gemei Liang Kongming Wu Shaohua Gu Yuyuan Guo Xinzhi Ni Xianchun Li | 2018 | Insect Science2018，25，4： | 2 |
| 4 | Preparation and characterization of some surface negatively charged residue mutants of cytochrome b_5显示文摘Site-directed mutagenesis was used to obtain seven variants of tryptic fragment of bovine liver cytochrome bs (cyt bs), in which the negatively charged residues around the heme exposed edge of cyt bs were replaced by hydropho-bic amino acid alanine. Double-site mutants, triple-site mutants and even quadruple-site mutants were obtained. DNA sequencing and molecular weight measurements of the mutant proteins both confirmed that these site-directed muta-genesises were successfully performed. Spectroelectrochem-istry of these mutant proteins revealed that the apparent redox potentials of these mutant proteins caused a positive shift of 2-10 mV. The global structure of these mutant proteins did not show much difference from that of the wild type cyt bs, providing a solid base for the further study on the roles of the proteins’ surface charges. | WANG Yunhua, WANG Wenhu, LU Junxia, REN Yi, GU Shaohua, XIE Yi & HUANG Zhongxian1. Chemical Biology Lab, Department of Chemistry, Fudan University, Shanghai 200433, China 2. Genetic Institute, School of Life, Fudan University, Shanghai 200433, China | 2001 | Chinese Science Bulletin2001，46，7： | 1 |
| 5 | A meta-analysis of DNA repair gene XPC polymorphisms and cancer risk显示文摘 | Deqiang Zhang Chengwen Chen Xuping Fu Shaohua Gu Yumin Mao Yi Xie Yan Huang Yao Li | 2008 | Journal of Human Genetics2008，，1： | 1 |
| 6 | Anchoring Single Nickel Atoms on Carbon-vacant Carbon Nitride Nanosheets for Efficient Photocatalytic Hydrogen Evolution显示文摘Polymeric carbon nitride(PCN)has emerged as a promising candidate for photocatalytic hydrogen evolution,but its dependence on scarce and high-cost noble metal co-catalysts severely limits its extensive application.It will be of great promise to develop non-noble metal single-atom co-catalysts with low-cost and high atom utilization to improve the photocatalytic performance over PCN.Herein,single Ni atoms are successfully anchored onto carbon-vacant PCN nanosheets(CCN-SANi)via a two-step ammonia thermal treatment and photo-deposition process.Theoretical calculations and experimental results demonstrate that the optical absorption property and the charge transfer ability of CCN-SANi have been significantly improved with the introduction of single Ni atoms to form Ni-N3 sites.In comparison to carbon-vacant PCN(CCN)loaded with Ni clusters,the obtained CCN-SANi exhibits 11.4 times increased photocatalytic performance,with the highest hydrogen evolution rate reaching 511μmol/(g·h),which is even 1.7 times higher than that of CCN loaded with Pt clusters.This research proposes an inspiring and reliable strategy to design novel single-atom semiconducting polymers with electronic structures manipulated for efficient photocatalysis. | LIN Zhi ZHANG Zhengqi WANG Yiqing PENG Zhiming WANG Xinxin WANG Ruizhe HUANG Yu-Cheng MENG Fanqi LI Mingtao DONG Chung-Li ZHANG Qinghua GU Lin SHEN Shaohua | 2022 | Chemical Research in Chinese Universities2022，38，5： | 1 |
| 7 | Functional characterizations of chemosensory proteins of the alfalfa plant bug Adelphocorls lineolatus indicate their involvement in host recognition显示文摘 | Gu Shaohua Wang Songying Zhang Xueying | 2012 | PLoS One2012，7，42： | 1 |
| 8 | Grid workflow based on dynamic modeling and scheduling显示文摘 | Gu Ning | 2004 | ITCC''042004，，： | 1 |
| 9 | Comparison of yield components and plant type characteristics of high-yield rice between Taoyuan, a ‘special eco-site’ and Nanjing, China显示文摘 | Ganghua Li Lihong Xue Wei Gu Congdang Yang Shaohua Wang Qihong Ling Xia Qin Yanfeng Ding | 2009 | Field Crops Research2009，，2： | 1 |
| 10 | Functional characterizations of chemosensory proteins of the Alfalfa plant bug Adelphocoris lineolatus indicate their involvement in host recognition显示文摘 | Gu Shaohua Wang Songying Zhang Xueying | 2013 | PLoS One2013，7，42： | 1 |
| 11 | Binding charac- terization of recombinant odorant-binding proteins from the parasitic wasp, Microplitis mediator(Hymenoptera: Braconidae) 显示文摘 | Zhang Shuai Chen Lizhen Gu Shaohua | 2011 | Journal of Chemical Ecology2011，37，2： | 1 |
| 12 | A Pipeline Fracture Model of Hydrogen-induced Cracking显示文摘The relationship between hydrogen concentration and crack tip stress, strain field, hydrogen diffusion and internal pressure respectively in the crack tip process zone was investigated, and the length of the crack tip process zone of hydrogen-induced cracking (HIC) was determined. Based on the mechanism of fracture of micro-crack nucleation, a dislocation model was presented for the fracture criteria of HIC. The influence factors of pipeline tube fracture ductile KISCC in the presence of hydrogen was analyzed, and the critical pressure bearing capability of a pipeline with hydrogen-induced cracking and the critical J-integrity (JISCC) were calculated, which is very important for pipeline safety. | Dong Shaohua Fan Fei Gu Zhiyu Lv Yingmin | 2006 | Petroleum Science2006，3，1： | 1 |
| 13 | Isolation and identification of a novel cDNA that encodes human yrdC protein显示文摘 | Jinzhong Chen Chaoneng Ji Shaohua Gu Enpeng Zhao Jianliang Dai Lu Huang Ji Qian Kang Ying Yi Xie Yumin Mao | 2003 | Journal of Human Genetics2003，，4： | 1 |
| 14 | Early Diagnosis of Bipolar Disorder Coming Soon:Application of an Oxidative Stress Injury Biomarker(BIOS)Model显示文摘Early distinction of bipolar disorder(BD)from major depressive disorder(MDD)is difficult since no tools are available to estimate the risk of BD.In this study,we aimed to develop and validate a model of oxidative stress injury for predicting BD.Data were collected from 1252 BD and 1359 MDD patients,including 64 MDD patients identified as converting to BD from 2009 through 2018.30 variables from a randomly-selected subsample of 1827(70%)patients were used to develop the model,including age,sex,oxidative stress markers(uric acid,bilirubin,albumin,and prealbumin),sex hormones,cytokines,thyroid and liver function,and glycolipid metabolism.Univariate analyses and the Least Absolute Shrinkage and Selection Operator were applied for data dimension reduction and variable selection.Multivariable logistic regression was used to construct a model for predicting bipolar disorder by oxidative stress biomarkers(BIOS)on a nomogram.Internal validation was assessed in the remaining 784 patients(30%),and independent external validation was done with data from 3797 matched patients from five other hospitals in China.10 predictors,mainly oxidative stress markers,were shown on the nomogram.The BIOS model showed good discrimination in the training sample,with an AUC of 75.1%(95%CI:72.9%–77.3%),sensitivity of 0.66,and specificity of 0.73.The discrimination was good both in internal validation(AUC 72.1%,68.6%–75.6%)and external validation(AUC 65.7%,63.9%–67.5%).In this study,we developed a nomogram centered on oxidative stress injury,which could help in the individualized prediction of BD.For better real-world practice,a set of measurements,especially on oxidative stress markers,should be emphasized using big data in psychiatry. | Zhiang Niu Xiaohui Wu Yuncheng Zhu Lu Yang Yifan Shi Yun Wang Hong Qiu Wenjie Gu Yina Wu Xiangyun Long Zheng Lu Shaohua Hu Zhijian Yao Haichen Yang Tiebang Liu Yong Xia Zhiyu Chen Jun Chen Yiru Fang | 2022 | Neuroscience Bulletin2022，38，9： | 1 |
| 15 | Delayed toe transplantation:experimental study and clinical application显示文摘 | GU Yudong LI Shaohua CHEN Desong | | 0，，05： | 1 |
| 16 | Construction of Ad-EGFP-BDNF vector and its expression in neural stem cells显示文摘BACKGROUND:Brain-derived neurotrophic factor(BDNF) provides nourishment to injured neurons.Neural stem cells can differentiate into neurons to repair neuronal injury in vivo.It has been hypothesized that continuous secretion of BDNF from neural stem cells could benefit brain injury repair.OBJECTIVE:To transfect BDNF and enhanced green fluorescent protein(EGFP) into neural stem cells with adenovirus vector and to observe expression of BDNF and EGFP in transfected neural stem cells.DESIGN,TIME AND SETTING:Observational,cellular,molecular study was performed at the Biochemistry Laboratory,Tongji University School of Medicine,China from July 2004 to September 2006.MATERIALS:Neural stem cells were provided by the Anatomy and Histoembryology Laboratory of Fudan University Medical School,China.METHODS:BDNF cDNA was extracted by reverse transcription polymerase chain reaction from the rat hippocampus.Following gene cloning and packaging by HEK293.BDNF,the EGFP gene was transfected into cultured neural stem cells with the Ad-EGFP-BDNF vector.BDNF-expressing neural stem cell clones were selected by G418 selection.MAIN OUTCOME MEASURES:EGFP expression and cell morphology were observed by fluorescent microscopy;neural stem cell expressing BDNF mRNA was examined by reverse transcription polymerase chain reaction;BDNF expression was detected by enzyme-linked immunosorbent assay from supernatant of infected neural stem cells.RESULTS:High transfection efficiency was obtained using 5 × 108 virus titers to transfect neural stem cells.G418-resistant neural stem cell clones integrated BDNF mRNA fragments.Enzyme-linked immunosorbent assay results showed that BDNF expression in the supernatant increased with increasing culture time and peaked at 72 hours.CONCLUSION:Adenovirus-mediated BDNF and EGFP genes were successfully transfected into neural stem cells and were expressed in neural stem cells for a long period of time. | Shaohua Li Zhenhua Li Huilong Wen Xin Gu Yinjiang Cui Yang Wang Lixia Lu Yanna Li Manli Na | 2010 | Neural Regeneration Research2010，5，13： | 1 |
| 17 | Analysis and countermeasures of the conflicting between clinical practice and taking test of postgraduate of medical undergraduates显示文摘Nowadays,with the expanding enrollment of medical schools in our country,the competitive pressures of employment increase.It is very difficult for the graduates of these schools to find ideal jobs,but medical students with master or doctor degrees are relatively optimistic to the employment situation.As a result,more and more fresh medical graduates pursue the post-graduate education,thus forms a'rush of taking test of postgraduate'phenomenon[1].Clinical students | Zhang Lixia Gu Yun Cai Heng Liang Shaohua Shao Yiye Ni Yanbo | 2014 | 重庆医学2014，43，25： | 1 |
| 18 | Multi-objective network optimization combining topology and routing algorithms in multi-layered satellite networks显示文摘In satellite communication studies, the multi-layered satellite network (MLSN) can take advan-tage of both wide coverage and low time delay.Furthermore, the issue of stable networking anddynamic reconstructing methods is still worth ex-ploring. However, current studies rarely involvethe combination of the routing algorithm and thenetwork topology. | Zhuoming LI Huiyun XIA Yu ZHANG Junqing QI Shaohua WU Shushi GU | 2018 | Science China(Information Sciences)2018，61，8： | 0 |
| 19 | Deep-Learning-Based Production Decline Curve Analysis in the Gas Reservoir through Sequence Learning Models显示文摘Production performance prediction of tight gas reservoirs is crucial to the estimation of ultimate recovery,which has an important impact on gas field development planning and economic evaluation.Owing to the model’s simplicity,the decline curve analysis method has been widely used to predict production performance.The advancement of deep-learning methods provides an intelligent way of analyzing production performance in tight gas reservoirs.In this paper,a sequence learning method to improve the accuracy and efficiency of tight gas production forecasting is proposed.The sequence learning methods used in production performance analysis herein include the recurrent neural network(RNN),long short-term memory(LSTM)neural network,and gated recurrent unit(GRU)neural network,and their performance in the tight gas reservoir production prediction is investigated and compared.To further improve the performance of the sequence learning method,the hyperparameters in the sequence learning methods are optimized through a particle swarm optimization algorithm,which can greatly simplify the optimization process of the neural network model in an automated manner.Results show that the optimized GRU and RNN models have more compact neural network structures than the LSTM model and that the GRU is more efficiently trained.The predictive performance of LSTM and GRU is similar,and both are better than the RNN and the decline curve analysis model and thus can be used to predict tight gas production. | Shaohua Gu Jiabao Wang Liang Xue Bin Tu Mingjin Yang Yuetian Liu | 2022 | Computer Modeling in Engineering & Sciences2022，，6： | 0 |
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